Journal articles: 'Cultivar Patativa'

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Dutra, Alek Sandro, Elizita Maria Teófilo, Sebstião Medeiros Filho, and Francisco Tiago Cunha Dias. "Qualidade fisiológica de sementes de feijão caupi em quatro regiões do Estado do Ceará." Revista Brasileira de Sementes 29, no. 2 (August 2007): 111–16. http://dx.doi.org/10.1590/s0101-31222007000200015.

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O presente trabalho foi realizado com o objetivo de avaliar a qualidade fisiológica das sementes de feijão caupi, colhidas em quatro regiões do Estado do Ceará. Foram utilizadas sementes das cultivares Sempre verde, Setentão, Patativa, Pingo de ouro, Aparecido e Epace-10. Essas sementes foram produzidas nos municípios cearenses de Quixadá, Pentecoste, Limoeiro do Norte e Morada Nova, durante o ano de 2004. Foram realizados os teste de germinação, primeira contagem de germinação, emergência de plântulas, índice de velocidade de emergência e de condutividade elétrica. Para a maioria das cultivares testadas, as sementes produzidas em Pentecoste e Limoeiro do Norte apresentaram qualidade fisiológica superior quando comparada com aquelas produzidas em Quixadá e Morada Nova; As sementes das cultivares Sempre Verde, Setentão, Pingo de Ouro e Aparecido obtiveram desempenho superior, as da cultivar Epace-10 desempenho intermediário e as da Patativa, desempenho inferior.

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Halfeld-Vieira, Bernardo de Almeida, Kátia de Lima Nechet, and Giovanni Ribeiro de Souza. "Ocorrência da mancha-bacteriana do feijão-caupi em Roraima e reação de cultivares." Summa Phytopathologica 37, no. 3 (September 2011): 127–30. http://dx.doi.org/10.1590/s0100-54052011000300008.

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A mancha-bacteriana, causada por Xanthomonas axonopodis pv. vignicola, é uma doença que apresenta potencial de dano à cultura do feijão-caupi. Esse trabalho teve como objetivos registrar a ocorrência do patógeno em Roraima e prover informações sobre a reação de cultivares de feijão-caupi à doença. As cultivares utilizadas foram BRS-Amapá, BR02-Bragança, BRS Guariba, BR17-Gurguéia, BRS Mazagão, BRS Milênio, BRS Patativa, Pitiúba, BR03-Tracuateua e Vita-7. Em casa-de-vegetação, o delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, cada repetição foi representada por duas plantas/vaso. Os parâmetros de avaliação foram período de incubação e severidade da doença aos 25 dias após a inoculação. O experimento de campo foi conduzido em delineamento de blocos ao acaso com 4 blocos, sendo cada um constituído por 10 parcelas, sendo cada parcela semeada com uma cultivar. As inoculações foram realizadas aos 35 dias após a semeadura, em estádio de início da emissão do botão floral. Os parâmetros avaliados foram período de incubação, severidade da doença aos 12, 18, 22, 27 e 29 dias após a inoculação. A transmissibilidade da bactéria por meio de sementes foi verificada a partir da deposição em placas de Petri contendo o meio 523 de alíquotas de 100 µl de suspensões obtidas de diluições seriadas em fator 1:10 de 150 g de sementes de cada lote. Verificou-se que as cultivares BRS Mazagão, BR 17- Gurguéia e Vita-7 apresentaram reação de resistência à mancha-bacteriana. Não foi verificada a ocorrência da transmissibilidade da bactéria nas condições experimentais.

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Barros, Gislanne Brito, Maria do Socorro da Rocha Nogueira, Cláudia Roberta Ribeiro de Oliveira, Francisco Rodrigues Freire Filho, Valdenir Queiroz Ribeiro, Carlos Frederico de Menezes Veiga, Paulo Sérgio Torres Brioso, and Marcelo Eiras. "Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus." Summa Phytopathologica 39, no. 2 (June 2013): 130–36. http://dx.doi.org/10.1590/s0100-54052013000200009.

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Dentre os vírus que infectam o feijão-caupi (Vigna unguiculata L. Walp.) destacam-se, respectivamente, pela severidade e ampla ocorrência o Cowpea severe mosaic virus (CPSMV) e o Cowpea aphid-borne mosaic virus (CABMV). Portanto, objetivaram-se, no presente trabalho, obter e avaliar plantas de feijão-caupi com resistência ao CPSMV e ao CABMV, visando ao desenvolvimento de cultivares essencialmente derivadas e novas cultivares. Realizaram-se oito cruzamentos seguidos de retrocruzamentos, utilizando a linhagem TE 97-309G-9 e a cultivar Patativa como genitores resistentes, e as cultivares BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba e Pretinho como genitores suscetíveis. As gerações F2 e F2RC1 foram desafiadas quanto à resistência por meio de inoculação mecânica com isolados do CPSMV e do CABMV. Nas gerações F2RC1, além da resistência foram avaliados os caracteres: número de dias para o início da floração, comprimento das vagens, número de grãos. vagem-1, peso de cem grãos e produção de grãos.planta-1. Todos os indivíduos F2 e F2RC1 foram analisados pelo teste χ² e se ajustaram à frequência esperada de 15 plantas suscetíveis 1 planta resistente a ambos os vírus. As médias das plantas F2RC1 resistentes, de cada retrocruzamento, foram comparadas com a média do seu respectivo genitor recorrente pelo teste 't' e as médias dos retrocruzamentos foram comparadas pelo teste de Scott-Knott. Foi detectada variabilidade genética entre os retrocruzamentos para todos os caracteres. Todos os retrocruzamentos foram considerados promissores para produção de cultivares essencialmente derivadas resistentes ao CPSMV e ao CABMV e as plantas selecionadas possuem características que possibilitam a seleção de linhagens com grãos de bom padrão comercial e altamente produtivas.

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Bárta, J., and V. Bártová. "Patatin, the major protein of potato (Solanum tuberosum L.) tubers, and its occurrence as genotype effect: processing versus table potatoes." Czech Journal of Food Sciences 26, No. 5 (October 31, 2008): 347–59. http://dx.doi.org/10.17221/27/2008-cjfs.

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Patatin relative abundance in SDS-extractable protein and patatin content in dry matter were evaluated in tubers of forty processing and table potato cultivars usually cultivated in the Czech Republic, Germany, and the Netherlands. The patatin characteristics were evaluated over three experimental years. Patatin relative abundance in the processing cultivars achieved on average a significantly higher value (<I>P</I> < 0.001; Tukey HSD test) than patatin relative abundance in the table cultivars, resulting in average values of 25.80% and 21.59%, respectively. A high patatin relative abundance (over 30% in extractable protein) was determined only in the case of two cultivars: Vaneda (average 31.29%) and Tomensa (average 31.24%). Patatin content in tuber dry matter was significantly higher in the processing potato cultivars in all three experimental years (<I>P</I> < 0.001), attaining a mean of 1.28% with the processing cultivars and 1.03% with the table cultivars. The direct effect of the cultivar on patatin relative abundance in SDS-extractable protein was higher (33.1% for processing potato cultivars and 48.1% for table potato cultivars) than the effect of the growing year (15.6% for processing potato cultivar and 22.8% for table potato cultivars).

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Nascimento, Cleópatra Saraiva do, Mario Andrade Lira Junior, Newton Pereira Stamford, Maria Betânia Galvão Santos Freire, and Clayton Albuquerque Sousa. "Nodulação e produção do caupi (Vigna unguiculata L. Walp) sob efeito de plantas de cobertura e inoculação." Revista Brasileira de Ciência do Solo 32, no. 2 (April 2008): 579–87. http://dx.doi.org/10.1590/s0100-06832008000200013.

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Um experimento de campo foi realizado no município de Crato - Ceará para estudar como a planta de cobertura afeta a nodulação e produtividade de caupi (Vigna unguiculata (L.) Walp), submetido a diferentes inoculações. Empregou-se o delineamento em blocos casualizados, no esquema em parcelas subsubdivididas, com quatro repetições. Os tratamentos consistiram de plantas de cobertura, formando a parcela principal; inoculação, como subparcela; e amostragens quinzenais, como subsubparcela. As plantas de cobertura foram: milheto - Pennisetum americanum; mucuna-preta - Stizolobium aterrimum ou vegetação espontânea, enquanto as fontes de N foram: sem inoculação; sem inoculação + 50 kg ha-1 de N na forma de uréia; inoculação com estirpes rizobianas (Bradyrhizobium sp.) recomendadas pela RELARE -BR 3301 + BR 3302; ou pela UFRPE - NFB 6156 + NFB 700. Foi utilizada a cultivar Patativa de caupi, recomendada para a região. A partir de sete dias após a emergência e até a maturidade final da cultura, foram realizadas amostras quinzenais, colhendo-se duas plantas, por subparcela para determinação do número de nódulos, matéria seca de nódulos e matéria seca da parte aérea. A partir dos 60 dias após emergência, foram realizadas colheitas semanais da área útil da subparcela, para determinação da produtividade de grãos. A mucuna aumentou a nodulação do caupi não-inoculado. A inoculação do caupi com estirpes recomendadas, ou o aumento da população nativa, permitiu produção de biomassa e grãos semelhante da adubação com 50 kg ha-1 de N.

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Li, Xiu-Qing, Muhammad Haroon, Shirlyn E. Coleman, Andrew Sullivan, Mathuresh Singh, Len Ward, Solke H. De Boer, Tieling Zhang, and Danielle J. Donnelly. "A simplified procedure for verifying and identifying potato cultivars using multiplex PCR." Canadian Journal of Plant Science 88, no. 4 (July 1, 2008): 583–92. http://dx.doi.org/10.4141/cjps07061.

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Correct identification of potato cultivars and selections is essential to a large and diverse user group. This group includes curators of germplasm repositories, breeders and other researchers, certification program officials, commercial growers, processing industry managers and for some cultivars, the public. Agencies involved in cultivar registration and plant breeders' rights (or patenting) also have a vested interest in correct identification. DNA fingerprinting is an important tool that can be used to describe new or existing cultivars, verify cultivar identity, and resolve cultivar mixtures. Gel-based fingerprints are usually preferred because they are visual and within the technical capacity of most molecular laboratories. In this study, a multiplex PCR protocol "Multiplex SUP" and an improved version "Multiplex SUPN" were developed using four primer pairs (STEM0014 and genes of starch synthase, patatin, and UDP-glucose pyrophosphorylase). The agarose-gel-based Multiplex SUP was successfully used in identifying cultivars from blind samples in a collaborating laboratory, and in pilot tests to verify the identity of introduced cultivars for seed potato production. The Multiplex SUPN, using native polyacrylamide gel electrophoresis (PAGE) with GelRed or ethidium-bromide staining, generated more than 38 polymorphic markers among the potato cultivars tested. The method distinguished 116 cultivars that included many of the public potato cultivars registered in Canada and several protected cultivars that were fingerprinted with permission. The Multiplex SUPN-PAGE method is user friendly and effective, and is recommended for routine potato cultivar verification and identification. Key words: cultivar identification, database development, DNA fingerprinting, GelRed staining, genotyping, polymorphism, Solanum tuberosum L.

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Racusen, David. "Esterase specificity of patatin from two potato cultivars." Canadian Journal of Botany 64, no. 9 (September 1, 1986): 2104–6. http://dx.doi.org/10.1139/b86-276.

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'Désirée' potato tubers contained about one-half the patatin of 'Kennebec', a typical commercial cultivar. The 'Désirée' patatin, like that of 'Kennebec', copurified with esterase activity, but it consisted of only two major ionic forms and its ability to hydrolyse p-nitrophenyl esters was relatively low. Despite these differences, the esterase of 'Désirée' patatin was quite active toward other substrates. Patatin isoforms, separated by isoelectric focusing, had the same esterase substrate preference as the cultivar-specific patatin from which they were derived.

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Matos, A. R., A. d'Arcy-Lameta, M. França, Y. Zuily-Fodil, and A. T. Pham-Thi. "A patatin-like protein with galactolipase activity is induced by drought stress in Vigna unguiculata leaves." Biochemical Society Transactions 28, no. 6 (December 1, 2000): 779–81. http://dx.doi.org/10.1042/bst0280779.

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This paper reports the cloning of a cDNA (Vupat 1) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were ex-amined in Northern-blot analyses. Expression of Vupat 1 is stimulated by drought stress, especially in the drought-sensitive cultivar. Vupat 1 was expressed in the baculovirus system as a fusion protein secreted in the culture medium. The recombinant protein displays lipolytic activity towards monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerols.

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Moraes, João Gutemberg Leite, and Ervino Bleicher. "Preferência do pulgão-preto, Aphis craccivora Koch, a diferentes genótipos de feijão-de-corda, Vigna unguiculata (L.) Walp." Ciência Rural 37, no. 6 (December 2007): 1554–57. http://dx.doi.org/10.1590/s0103-84782007000600008.

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Este trabalho foi desenvolvido visando a avaliar a resposta de cultivares de feijão-de-corda, Vigna unguiculata (L.) Walp., à presença do pulgão-preto (Aphis craccivora Koch). Os experimentos foram conduzidos de agosto a outubro de 2004, em casa-de-vegetação da Universidade Federal do Ceará (UFC). As cultivares foram: "Epace-10", "Epace-11", "Patativa", "Pingo de Ouro", "Pitiúba", "BR-10 Piauí", "BR-12 Canindé", "BR-14 Mulato" e "BR-17 Gurguéia". O experimento constou de três ensaios, cada um com cinco tratamentos e seis repetições, sendo o delineamento estatístico inteiramente casualizado. Os genótipos foram cultivados em copos plásticos de 300ml e mantidos em gaiolas protegidas por tela antiafídeos. As plantas foram infestadas após doze dias do plantio através da liberação de cinco fêmeas adultas do pulgão-preto por planta. As avaliações foram realizadas após o terceiro e o quinto dia da infestação, constando da contagem direta das formas adulta e jovem do inseto presentes nas plantas. Os dados obtidos foram analisados através de análise de variância e pelo teste de Tukey em 5% de probabilidade de erro. As cultivares "Epace-10" e "Patativa" foram as menos preferidas por A. craccivora.

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Bernal, Javier, Daniel Mouzo, María López-Pedrouso, Daniel Franco, Lucio García, and Carlos Zapata. "The Major Storage Protein in Potato Tuber Is Mobilized by a Mechanism Dependent on Its Phosphorylation Status." International Journal of Molecular Sciences 20, no. 8 (April 17, 2019): 1889. http://dx.doi.org/10.3390/ijms20081889.

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The role of the protein phosphorylation mechanism in the mobilization of vegetative storage proteins (VSPs) is totally unknown. Patatin is the major VSP of the potato (Solanum tuberosum L.) tuber that encompasses multiple differentially phosphorylated isoforms. In this study, temporal changes in the phosphorylation status of patatin isoforms and their involvement in patatin mobilization are investigated using phosphoproteomic methods based on targeted two-dimensional electrophoresis (2-DE). High-resolution 2-DE profiles of patatin isoforms were obtained in four sequential tuber life cycle stages of Kennebec cultivar: endodormancy, bud break, sprouting and plant growth. In-gel multiplex identification of phosphorylated isoforms with Pro-Q Diamond phosphoprotein-specific stain revealed an increase in the number of phosphorylated isoforms after the tuber endodormancy stage. In addition, we found that the phosphorylation status of patatin isoforms significantly changed throughout the tuber life cycle (P < 0.05) using the chemical method of protein dephosphorylation with hydrogen fluoride-pyridine (HF-P) coupled to 2-DE. More specifically, patatin phosphorylation increased by 32% from endodormancy to the tuber sprouting stage and subsequently decreased together with patatin degradation. Patatin isoforms were not randomly mobilized because highly phosphorylated Kuras-isoforms were preferably degraded in comparison to less phosphorylated non-Kuras isoforms. These results lead us to conclude that patatin is mobilized by a mechanism dependent on the phosphorylation status of specific isoforms.

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Racusen, David, and Richard Racusen. "Esterase activity and structural heterogeneity in patatin from a wild potato species." Canadian Journal of Botany 70, no. 3 (March 1, 1992): 597–600. http://dx.doi.org/10.1139/b92-077.

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Analysis of protein extracts of tubers from the potato species Solanum berthaultii revealed that they contain two types of patatin. One was a highly glycosylated form that exhibited weak esterase activity, and the other resembled the low-carbohydrate – strong esterase form that is typical of most potato cultivars. Though the function of patatin is unknown, the production of high- and low-carbohydrate forms may be under some form of developmental regulation; we observe considerable variation in the levels of expression in tubers taken from single plants. Key words: patatin, glycoprotein, esterase, Solanum berthaultii, Solanum tuberosum.

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Huai-Jun, Si, Liu Jun, and Xie Cong-Hua. "Transformation of potato using an antisense class I patatin gene and its effect on microtuber formation." Chinese Journal of Agricultural Biotechnology 2, no. 1 (April 2005): 7–11. http://dx.doi.org/10.1079/cjb200544.

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AbstractAn antisense class I patatin gene under control of the CaMV 35S promoter was introduced into potato (Solanum tuberosum) cultivar E-potato 3 using the Agrobacterium tumefaciens system. PCR amplification and PCR–Southern blot analysis indicated that the antisense class I patatin gene had been integrated into the potato genome. Northern hybridization analysis showed that the antisense gene transcribed normally in the transgenic potato plants and resulted in a reduction of endogenous class I patatin mRNA. Total soluble protein content and lipid acyl hydrolase activity of microtubers, derived from transformed plants, decreased by a maximum of 36.4% and 31.4%, respectively, compared with control plants. The expression of this antisense gene also resulted in reductions of the plantlets forming tubers, tubers per plantlet and the effective tubers (≥50 mg) of the transformed plants.

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Marsaro Júnior, Alberto Luiz, and Aloisio Alcantara Vilarinho. "Resistência de cultivares de feijão-caupi ao ataque de Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) em condições de armazenamento." Revista Acadêmica: Ciência Animal 9, no. 1 (January 15, 2011): 51. http://dx.doi.org/10.7213/cienciaanimal.v9i1.11078.

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Callosobruchus maculatus é uma das principais pragas do feijão-caupi armazenado no Brasil. Estudos queavaliem a resistência de genótipos de feijão-caupi com relação ao ataque dessa praga são raros na regiãonorte do Brasil. Nesse sentido, o objetivo desta pesquisa foi avaliar a resistência de cultivares de feijãocaupicom relação ao ataque de C. maculatus, em condições de armazenamento, no Estado de Roraima.Amostras de grãos de doze cultivares de feijão-caupi, BRS Mazagão, BRS Paraguaçu, BRS Novaera, BRSGuariba, BRS Milênio, BRS Cauamé, BR 3 Tracuateua, BR 17 Gurguéia, Vita 7, Amapá, Patativa e Linhagem26, foram infestadas com adultos de C. maculatus e mantidas em condições de laboratório a 25 °C e 12horas de fotofase. Após 20 dias da infestação foram avaliados os seguintes parâmetros de resistência: ciclobiológico, perda de massa seca dos grãos e número de insetos emergidos. As cultivares mais suscetíveis aoataque de C. maculatus foram: BRS Mazagão e Vita 7, enquanto as mais resistentes foram: BR 17 Gurguéiae BRS Cauamé.

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Bezerra, Júlia Medeiros, Maria Marlene da Silva Vieira, Adriana Ferreira dos Santos, Emanuel Tarcísio do Rêgo Farias, Maíra Felinto Lopes, and Anielson dos Santos Souza. "Composição química de oito cultivares de feijão-caupi." Revista Verde de Agroecologia e Desenvolvimento Sustentável 14, no. 1 (January 1, 2019): 41. http://dx.doi.org/10.18378/rvads.v14i1.5683.

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O feijão é um dos alimentos mais produzidos em todo o território nacional, sendo intensa a busca por cultivares produtivas, com boas características culinárias e diferentes propriedades nutricionais. Com o presente estudo, visou-se avaliar a influencia do cozimento na composição centesimal de oito cultivares de feijão-caupi: Costela de Vaca, BRS Marataoã, BRS Itaim, BR 17-Gurguéia, BRS Novaera, Paulistinha, Setentão e Patativa. Os grãos foram submetidos a dois procedimentos diferentes para realização das análises: in natura (farinha) e cozido (com maceração) sob pressão. Foram realizadas as determinações de umidade, cinzas, lipídios, proteínas, carboidratos, valor energético, pH, acidez e açúcares. Todas as análises foram realizadas em quadruplicata nas cultivares in natura e cozidas, sendo os resultados expressos como média ± desvio-padrão. Os resultados mostraram que o conteúdo de umidade variou de 7-11% nas cultivares cruas, com aumento após o cozimento (60-70%). Ao passo que, o conteúdo de proteínas, cinzas, carboidratos, valor energético e açúcares, diminuíram em todas as cultivares cozidas. Pode-se concluir que, dentre as cultivares analisadas, a Setentão foi a que apresentou o maior teor de proteína, antes e após o cozimento. Mesmo com redução nos demais parâmetros, as cultivares de feijão-caupi mantiveram características nutritivas e funcionais relevantes, apresentando um tempo de cocção entre 8 a 10 minutos, sendo consideradas de rápido cozimento e, portanto adequadas para atender a demanda atual dos consumidores.

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Da Ros, Letitia, Raed Elferjani, Raju Soolanayakanahally, Sateesh Kagale, Shankar Pahari, Manoj Kulkarni, Jazeem Wahab, and Benoit Bizimungu. "Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers." Genes 11, no. 8 (July 30, 2020): 864. http://dx.doi.org/10.3390/genes11080864.

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Competition for scarce water resources and the continued effects of global warming exacerbate current constraints on potato crop production. While plants’ response to drought in above-ground tissues has been well documented, the regulatory cascades and subsequent nutritive changes in developing tubers have been largely unexplored. Using the commercial Canadian cultivar “Vigor”, plants were subjected to a gradual drought treatment under high tunnels causing a 4 °C increase in the canopy temperature. Tubers were sampled for RNAseq and metabolite analysis. Approximately 2600 genes and 3898 transcripts were differentially expressed by at least 4-fold in drought-stressed potato tubers, with 75% and 69% being down-regulated, respectively. A further 229 small RNAs were implicated in gene regulation during drought. Expression of several small RNA clusters negatively correlated with expression of their six target patatin genes, suggesting involvement in the regulation of storage proteins during drought. The comparison of protein homologues between Solanum tuberosum L. and Arabidopsis thaliana L. indicated that down-regulated genes were associated with phenylpropanoid and carotenoid biosynthesis. As is indicative of reduced flow through the phenylpropanoid pathway, phenylalanine accumulated in drought-stressed tubers. This suggests that there may be nutritive implications to drought stress occurring during the potato tuber bulking phase in sensitive cultivars.

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Bárta, Jan, Veronika Bártová, Zbyněk Zdráhal, and Ondrej Šedo. "Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.)." Journal of Agricultural and Food Chemistry 60, no. 17 (April 21, 2012): 4369–78. http://dx.doi.org/10.1021/jf3003448.

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Blundy, K. S., M. A. C. Blundy, D. Carter, F. Wilson, W. D. Park, and M. M. Burrell. "The expression of class I patatin gene fusions in transgenic potato varies with both gene and cultivar." Plant Molecular Biology 16, no. 1 (January 1991): 153–60. http://dx.doi.org/10.1007/bf00017925.

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Ruiz de Galarreta et al, J. "Evaluación del valor nutricional de germoplasma nativo de patata para su incorporación en programas de mejora genética." Revista Latinoamericana de la Papa 15, no. 1 (May 10, 2016): 55–58. http://dx.doi.org/10.37066/ralap.v15i1.153.

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La patata (Solanum tuberosum L.) es una fuente de carbohidratos digeribles, proteínas, nutrientes esenciales y vitaminas, lo que la convierte en un alimento muy completo. No obstante uno de los fines de los programas de mejora es aumentar el valor nutricional. El objetivo de este trabajo ha sido identificar variedades nativas de patata de diferentes especies, que portan caracteres de interés para su incorporación posterior en S. tuberosum. Para ello se analizaron la proteína bruta, macro y mircronutrientes, cenizas, materia seca, capacidad antioxidante, glicoalcaloides, azúcares reductores, contenido en almidón y aptitud para fritura francesa y para chips en 53 cultivares del G. Solanum. Se ha obtenido para todos los parámetros una amplia variabilidad. La proteína bruta presenta mayores valores en la especie S. tuberosum, en cambio tanto los micros y los macronutrientes presentan sus máximos valores en las diferentes especies nativas cultivadas. Las variedades más prometedoras se incorporarán como genitores en el programa de mejora genética que se lleva a cabo en nuestro Instituto.

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Vosman, B., and P. Arens. "Molecular characterization of GATA/GACA microsatellite repeats in tomato." Genome 40, no. 1 (February 1, 1997): 25–33. http://dx.doi.org/10.1139/g97-004.

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Microsatellite repeats like GATA or GACA display a degree of variability that allows their use in cultivar identification. Southern hybridization with oligonucleotide probes complementary to these microsatellites were used for the detection of polymorphisms. To understand the molecular structure of the detected DNA, fragments hybridizing to GATA and GACA probes were cloned and sequenced. In the four clones analyzed, repeats of GATA and GACA were found intertwined. The GATA and GACA arrays were not perfect but were heavily degenerated, in that they contained many tetranucleotides that might have been derived by a single point mutation from GATA or GACA. Some of these derived sequences, like GGTA and GGAT, were present as relatively long stretches that also contained some point mutations. This supports the hypothesis that long stretches of repeats are stabilized by the accumulation of point mutations. Analysis of the flanking sequences of the fragments obtained with the GACA probe showed that one of them was homologous to a Lilium henryi retrotransposon and the other to a sequence upstream of a potato patatin gene. The two fragments obtained using the GATA probe were flanked by DNA that had no homology to any known sequence but they were highly homologous to each other. This DNA was frequently associated with GATA elements and was present in the tomato genome in approximately 4300 copies. The function of this new class of repetitive DNA, here termed U30, is presently unknown.Key words: simple sequence repeats, Lycopersicon esculentum, cultivar identification, repetitive DNA.

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Shan-Han, Cheng, Liu Jun, Xie Cong-Hua, Song Bo-Tao, and Li Jing-Cai. "Role of tobacco vacuolar invertase regulated by patatin promoter in potato tuber resistance to cold sweetening." Chinese Journal of Agricultural Biotechnology 4, no. 1 (April 2007): 81–86. http://dx.doi.org/10.1017/s1479236207001313.

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AbstractTo slow down the accumulation of reducing sugar in potato tubers exposed to low-temperature storage, an expression vector, pBICNI, including Nt-VIF (Nicotiana tabacum vacuolar inhibitor of beta fructosidase, a vacuolar invertase inhibitor from Nicotiana tabacum) gene regulated by potato tuber specific promoter class I patatin promoter (CIPP) was constructed and transformed into potato (Solanum tuberosum L.) cultivar E-potato 3 (E3). Detection by polymerase chain reaction (PCR), Northern and Southern hybridizations indicated that the full-length Nt-VIF cDNA was transformed successfully into cv. E3. After storing potato tubers of 14 transgenic lines at 4 or 20°C for 30 days, their activities of vacuolar invertase (VI) and reducing sugar (RS) content were analysed. The results showed that there were no significant differences in RS content between transgenic and untransformed (control) tubers stored at 20°C. However, RS content of transgenic lines was obviously reduced at 4°C compared to the control, from 34.0% (line B-13) to 76.8% reduction (line B-1), implying that VI activity was inhibited by Nt-VIF cDNA expression and RS content was reduced. Further analysis revealed a positive linear relationship between VI activity and RS content (VI=0.308RS+0.067), and lines B-1, B-2, B-6, B-9 and B-14 could meet the requirements of potato chips in terms of their low RS content after cold storage.

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Douches, D. S., T. J. Kisha, J. J. Coombs, W. Li, W. L. Pett, and E. J. Grafius. "Effectiveness of Natural and Engineered Host Plant Resistance in Potato to the Colorado Potato Beetle." HortScience 36, no. 5 (August 2001): 967–70. http://dx.doi.org/10.21273/hortsci.36.5.967.

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The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most serious insect pest of potatoes throughout the eastern and north central United States. Host plant resistance to the Colorado potato beetle has been identified in wild Solanum species and Bt-transgenic potato lines. Detached-leaf bioassays (72 h) were conducted on insecticide-resistant, first instar Colorado potato beetles to study the effectiveness of individual and combined host plant resistance traits in potato. Potato lines tested include non-transgenic cultivars (`Russet Burbank', `Lemhi Russet', and `Spunta'), a line with glandular trichomes (NYL235-4), a line with high foliar leptines (USDA8380-1), and transgenic lines expressing either codon-modified Bt-cry3A or Bt-cry5 (Bt-cry1Ia1). Bt-cry3A transgenic lines, foliar leptine line, and foliar leptine lines with Bt-cry5 had reduced feeding compared to non-transgenic cultivars. Glandular trichome lines and glandular trichome lines with Bt-cry5 did not reduce feeding in this no-choice feeding study. Some Bt-cry5 transgenic lines, using either the constitutive promoters CaMV35s or (ocs)3mas (Gelvin super promoter), were moderately effective in reducing larval feeding. Feeding on Bt-cry5 transgenic lines with the tuber-specific patatin promoter was not significantly different than or greater than feeding on the susceptible cultivars. Mortality of first instars was highest when fed on the Bt-cry3A lines (68% to 70%) and intermediate (38%) on the Bt-cry5 `Spunta' line SPG3 where the gus reporter gene was not included in the gene construct. Host plant resistance from foliar leptines is a candidate mechanism to pyramid with either Bt-cry3A or Bt-cry5 expression in potato foliage against Colorado potato beetle. Without multiple sources of host plant resistance, long-term sustainability is questionable for a highly adaptable insect like the Colorado potato beetle.

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Cazar, María, Paulina Villena, Juan Parra, Virgilio Espinoza, Giovanni Larriva, and Adriana Caldas. "Eficacia de extracto etanólico de eucalipto (Eucaliptus globulus) en el control de Alternaria sp. en cultivos de col y patata." MASKANA 5, no. 1 (June 25, 2014): 33–41. http://dx.doi.org/10.18537/mskn.05.01.03.

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Pots, Andr� M., Harry Gruppen, Rob van Diepenbeek, Johannes J. van der Lee, Martinus A?J?S van Boekel, Gerrit Wijngaards, and Alphons G?J Voragen. "The effect of storage of whole potatoes of three cultivars on the patatin and protease inhibitor content; a study using capillary electrophoresis and MALDI-TOF mass spectrometry." Journal of the Science of Food and Agriculture 79, no. 12 (September 1999): 1557–64. http://dx.doi.org/10.1002/(sici)1097-0010(199909)79:12<1557::aid-jsfa375>3.0.co;2-k.

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Aminedi, Raghavendra, and Niranjan Das. "Class I patatin genes from potato (Solanum tuberosum L.) cultivars: molecular cloning, sequence comparison, prediction of diverse cis-regulatory motifs, and assessment of the promoter activities under field and in vitro conditions." In Vitro Cellular & Developmental Biology - Plant 50, no. 6 (July 16, 2014): 673–87. http://dx.doi.org/10.1007/s11627-014-9631-3.

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Journal articles on the topic 'Cultivar Patativa'

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