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Journal articles on the topic 'Cultivated fibroblasts'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 February 2022

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1

Pigeolet, E., M. Raes, A. Houbion, and J. Remacle. "Effect of procaine on cultivated human WI-38 fibroblasts." Experimental Gerontology 23, no. 2 (1988): 87–96. http://dx.doi.org/10.1016/0531-5565(88)90073-3.

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2

Levina, Elina M., Margarita A. Kharitonova, Yuri A. Rovensky, and Jury M. Vasiliev. "Cytoskeletal control of fibroblast length: experiments with linear strips of substrate." Journal of Cell Science 114, no. 23 (2001): 4335–41. http://dx.doi.org/10.1242/jcs.114.23.4335.

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In order to understand the factors determining the length of fibroblasts, three cell lines (mouse embryonic fibroblasts plus human fibroblast lines AGO 1523 and M19) were cultivated on the usual planar substrate (glass) and on specially prepared narrow linear strips of the same substrate, where the cells could spread only linearly. Morphometric measurements showed that the average length of cells of each type on the ‘unidimensional’ strips was no different from that on the usual ‘bidimensional’ substrate. The addition of colcemid significantly decreased cell length on both substrates, whereas

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3

Tansaz, Samira, Raminder Singh, Iwona Cicha, and Aldo Boccaccini. "Soy Protein-Based Composite Hydrogels: Physico-Chemical Characterization and In Vitro Cytocompatibility." Polymers 10, no. 10 (2018): 1159. http://dx.doi.org/10.3390/polym10101159.

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Novel composite hydrogels based on the combination of alginate (Alg), soy protein isolate (SPI) and bioactive glass (BG) nanoparticles were developed for soft tissue engineering. Human umbilical vein endothelial cells (HUVEC) and normal human dermal fibroblasts were cultivated on hydrogels for 7, 14 and 21 days. Cell morphology was visualized using fluorescent staining at Days 7 and 14 for fibroblast cells and Days 14 and 21 for HUVEC. Metabolic activity of cells was analyzed using a colorimetric assay (water soluble tetrazolium (WST) assay). Compared to pure Alg, Alg/SPI and Alg/SPI/BG provid

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4

Bongenhielm, U., A. Hægerstrand, E. Theodorsson, and K. Fried. "Effects of neuropeptides on growth of cultivated rat molar pulp fibroblasts." Regulatory Peptides 60, no. 2-3 (1995): 91–98. http://dx.doi.org/10.1016/0167-0115(95)00115-8.

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5

Doillon, Charles J., Arthur J. Wasserman, Richard A. Berg, and Frederick H. Silver. "Behaviour of fibroblasts and epidermal cells cultivated on analogues of extracellular matrix." Biomaterials 9, no. 1 (1988): 91–96. http://dx.doi.org/10.1016/0142-9612(88)90077-4.

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6

Letsiou, Sophia, Artemis Bakea, Géraldine Le Goff, et al. "Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress." Marine Drugs 18, no. 9 (2020): 460. http://dx.doi.org/10.3390/md18090460.

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The strain Aspergillus chevalieri TM2-S6 was isolated from the sponge Axinella and identified according to internal transcribed spacer (ITS) molecular sequence homology with Aspergillus species from the section Restricti. The strain was cultivated 9 days on potato dextrose broth (PDB), and the medium evaluated as antioxidant on primary normal human dermal fibroblasts (NHDF). The cultivation broth was submitted to sterile filtration, lyophilized and used without any further processing to give the Aspergillus chevalieri TM2-S6 cultivation broth ingredient named ACBB. ACCB contains two main compo

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Bonatti, Silvilena, Bernardo Hochman, Vanina Monique Tucci-Viegas, et al. "In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts." Acta Cirurgica Brasileira 26, no. 1 (2011): 25–30. http://dx.doi.org/10.1590/s0102-86502011000100006.

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Purpose: To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concernin

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Yartsev, Vasily, Anna Gabashvili, Eugenia Atkova, Pavel Melnikov, and Tatiana Nesterova. "Study of Dosage-Dependent Effects of Cytostatic Drugs Using a Fibroblast Cell Culture of the Human Nasal Mucosa." International Archives of Otorhinolaryngology 24, no. 02 (2020): e206-e210. http://dx.doi.org/10.1055/s-0039-1697996.

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Abstract Introduction Knowing a concentration at which cytostatic drugs are toxic for the nasal fibroblasts will enable the use cytostatic drugs in the clinical practice to prevent excessive cicatrization. Objective To determine the cytostatic concentrations of mitomycin С, doxorubicin, and 5-fluorouracil affecting nasal mucosa fibroblasts. Methods We obtained material during an endonasal dacryocystorhinostomy with the patient's informed consent. The cells were cultivated. Second- to fourth-passage cells were used in the experiments. The cells were stained for vimentin and cluster of different

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9

Van Gansen, Paulette, and Nadine Van Lerberghe. "Potential and limitations of cultivated fibroblasts in the study of senescence in animals. A review on the murine skin fibroblasts system." Archives of Gerontology and Geriatrics 7, no. 1 (1988): 31–74. http://dx.doi.org/10.1016/0167-4943(88)90021-0.

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10

Markhoff, Jana, Martin Krogull, Christian Schulze, Christian Rotsch, Sandra Hunger, and Rainer Bader. "Biocompatibility and Inflammatory Potential of Titanium Alloys Cultivated with Human Osteoblasts, Fibroblasts and Macrophages." Materials 10, no. 1 (2017): 52. http://dx.doi.org/10.3390/ma10010052.

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11

LARSSON, ERIK, and ULF T. BRUNK. "TEM AND SEM FINDINGS IN CAT FIBROBLASTS CULTIVATED IN VITRO WITH AND WITHOUT MYCOPLASMA." Acta Pathologica Microbiologica Scandinavica Section A Pathology 89A, no. 1-6 (2009): 9–15. http://dx.doi.org/10.1111/j.1699-0463.1981.tb00180.x.

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12

Umeno, Y., A. Okuda, and G. Kimura. "Proliferative behaviour of fibroblasts in plasma-rich culture medium." Journal of Cell Science 94, no. 3 (1989): 567–75. http://dx.doi.org/10.1242/jcs.94.3.567.

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We examined the proliferative behaviour in tertiary culture cells of human skin fibroblasts (HSF) as well as cells of the rat 3Y1 diploid fibroblast line placed on a plastic substratum in a nutrient-rich medium containing high concentrations of platelet-poor plasma (PPP). Autochthonous human PPP was used for the HSF cells and heat-treated (at 56 degrees C for 30 min) bovine PPP was used for the 3Y1 cells. In both types of cells, the saturation cell density rose with increasing PPP concentration and reached a plateau at 30–90% PPP. When the cells were cultivated in serum, the saturation densiti

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13

Edhager, Anders V., Vibeke Stenbroen, Nadia Sukusu Nielsen, et al. "Proteomic investigation of cultivated fibroblasts from patients with mitochondrial short-chain acyl-CoA dehydrogenase deficiency." Molecular Genetics and Metabolism 111, no. 3 (2014): 360–68. http://dx.doi.org/10.1016/j.ymgme.2014.01.007.

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14

Castagnino, C. G., and M. I. Berria. "Influence of cell fixatives and incubation schedule on PAP labeling of fibronectin in cultivated fibroblasts." Journal of Histochemistry & Cytochemistry 34, no. 10 (1986): 1331–33. http://dx.doi.org/10.1177/34.10.3528285.

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A simple combined method for differential PAP labeling of fibronectin (FN) in mouse embryo fibroblast cultures was developed. Methanol-5% glacial acetic acid in dry ice-fixed cell monolayers showed mainly intracellular FN staining. Fixation with neutral paraformaldehyde before labeling, developed membrane- and extracellular matrix-associated FN. A combination of both procedures, which required incubation with primary antibody, fixation with paraformaldehyde followed by chilled acid methanol, and re-incubation with primary antibody, yielded sharp intracellular and extracellular FN labeling. The

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Buravsky, A. V., E. V. Baranov, S. I. Tretyak, and Z. B. Kvacheva. "Influence of Monochromatic Polarised Light-Emitting Diode Radiation on the Proliferation of Cultivated Human Dermal Fibroblasts." Novosti Khirurgii 23, no. 3 (2015): 247–55. http://dx.doi.org/10.18484/2305-0047.2015.3.247.

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Schmitz, Lisa, Belinda Berdien, Edith Huland, et al. "The Impact of a New Interleukin-2-Based Immunotherapy Candidate on Urothelial Cells to Support Use for Intravesical Drug Delivery." Life 10, no. 10 (2020): 231. http://dx.doi.org/10.3390/life10100231.

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(1) Background: The intravesical instillation of interleukin-2 (IL-2) has been shown to be very well tolerated and promising in patients with bladder malignancies. This study aims to confirm the use of a new IL-2 containing immunotherapy candidate as safe for intravesical application. IL-2, produced in mammalian cells, is glycosylated, because of its unique solubility and stability optimized for intravesical use. (2) Materials and Methods: Urothelial cells and fibroblasts were generated out of porcine bladder and cultured until they reached second passage. Afterwards, they were cultivated in r

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Seol, Dong Lim, Won Hee Jang, Sung Jae Lee, and Young Il Yang. "Fibrin-Reinforced Collagen Matrix Enhances Tissue Regeneration and Angiogenesis." Key Engineering Materials 288-289 (June 2005): 257–60. http://dx.doi.org/10.4028/www.scientific.net/kem.288-289.257.

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The goal of this study was to investigate effects of fibrin reinforcement of collagen sponges on fibroblasts-mediated contraction and in vivo tissue regeneration, especially angiogenesis. Human dermal fibroblasts (HDFs)-populated collagen sponges reinforced with or without fibrin were cultivated via the free-floating method in vitro. They were then evaluated using xenogeneic implantation into nude mice. The HDFs-populated collagen sponges reinforced with fibrin exhibited significantly decreased HDFs-mediated contraction in vitro (p<0.05). Microvascular and cellular densities of the collagen

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18

Rudolf, Emil, Jan Peychl, and Miroslav Červinka. "Toxic Effects of Chromium Acetate Hydroxide on Cells Cultivated In Vitro." Alternatives to Laboratory Animals 29, no. 2 (2001): 163–77. http://dx.doi.org/10.1177/026119290102900209.

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Many human activities, particularly industrial ones, result in an ever-growing production of toxic waste materials. The dynamics of the toxic effects of chromium acetate hydroxide, which is found in high concentrations in a waste sediment produced in the Czech Republic, were assessed by using a battery of in vitro tests carried out on two cell lines: L-929 (mouse fibroblasts) and Hep 2 (human laryngeal cells). Various markers of cell damage were assessed by phase-contrast, video and fluorescence microscopy, fluorometry, and DNA analysis. Chromium acetate hydroxide, over a concentration range o

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19

Konstantinova, Mariya Valer’yevna, Nikolay Valentinovich Khaytsev, Aleftina Alekseevna Kravtsova, and Lev Dmitrievich Balashov. "Skin wounds’ healing basic problems and the use of skin substitutes." Pediatrician (St. Petersburg) 6, no. 2 (2015): 85–95. http://dx.doi.org/10.17816/ped6285-95.

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Kin substitutes present a heterogenous group of substances that aid in temporary or permanent covering of wounds of various types. Although they can not replace surgical debridement or standard methods of treatment they proffer an alternative to standard methods of treatment whenever the latter are ineffective. Skin substitutes require less wound vascularization, they increase the wound’s cutaneous component, decrease or eliminate inhibitory factors, decrease inflammatory process and grant quick and safe wound closing. Mast cells besides regulating vascular reactions in trauma zone also boost

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20

Dobrowolski, Dariusz, Boguslawa Orzechowska-Wylegala, Bogumil Wowra, et al. "Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/281870.

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Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients.Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in

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21

Vasko, Radovan, Michael Koziolek, Masami Ikehata, et al. "Role of basic fibroblast growth factor (FGF-2) in diabetic nephropathy and mechanisms of its induction by hyperglycemia in human renal fibroblasts." American Journal of Physiology-Renal Physiology 296, no. 6 (2009): F1452—F1463. http://dx.doi.org/10.1152/ajprenal.90352.2008.

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Basic fibroblast growth factor (FGF-2) plays a role in renal fibrogenesis, although its potential implications for tubulointerstitial involvement in diabetic nephropathy are unknown. We evaluated the expression of FGF-2 in kidney biopsies from patients with diabetic nephropathy and studied the mechanisms of its induction in human renal fibroblasts under hyperglycemia. Tubulointerstitial expression of FGF-2 was significantly upregulated in diabetic nephropathy compared with control kidneys with a good correlation to the degree of the injury. Fibroblasts cultivated in high glucose displayed incr

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22

Brantová, Olga, Markéta Tesařová, Hana Hansíková, Milan Elleder, Jiří Zeman, and Jana Sládková. "Ultrastructural Changes of Mitochondria in the Cultivated Skin Fibroblasts of Patients with Point Mutations in Mitochondrial DNA." Ultrastructural Pathology 30, no. 4 (2006): 239–45. http://dx.doi.org/10.1080/01913120600820112.

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23

Blinova, M. "Effect of melanins from black yeast fungi on proliferation and differentiation of cultivated human keratinocytes and fibroblasts." Cell Biology International 27, no. 2 (2003): 135–46. http://dx.doi.org/10.1016/s1065-6995(02)00297-4.

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24

WIEDEMANN, FALK ROBERT, KIRSTIN WINKLER, HARTMUT LINS, CLAUS-W. WALLESCH, and WOLFRAM S. KUNZ. "Detection of Respiratory Chain Defects in Cultivated Skin Fibroblasts and Skeletal Muscle of Patients with Parkinson's Disease." Annals of the New York Academy of Sciences 893, no. 1 OXIDATIVE/ENE (1999): 426–29. http://dx.doi.org/10.1111/j.1749-6632.1999.tb07870.x.

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25

Higa, Kazunari, Yoshiyuki Satake, and Jun Shimazaki. "The characterization of human oral mucosal fibroblasts and their use as feeder cells in cultivated epithelial sheets." Future Science OA 3, no. 4 (2017): FSO243. http://dx.doi.org/10.4155/fsoa-2017-0074.

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26

Dahse, Regine, and Hartwig Kosmehl. "CELL SEPARATION AND GENE EXPRESSION ANALYSIS IN A TUMOR-STROMA INTERACTION MODEL." Image Analysis & Stereology 23, no. 3 (2011): 153. http://dx.doi.org/10.5566/ias.v23.p153-157.

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A novel technique for co-culturing and separating fibroblasts and carcinoma cells in a 2-D model of tumorstroma interaction is presented. The methodology is based on cell co-cultivation on an 1.35 μm thin membrane followed by rapid immunostaining and microdissection of the different cell compartments using a laser microdissection system (P.A.L.M. Microlaser Technologies AG, Germany). For identifying the tumor cell compartment, immunolabeling for a marker that is expressed only in epithelial tumor cells is performed. The RNA quality from the microdissected co-cultured cells was successfully pro

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Sikorska-Zimny, Kalina, Paweł Lisiecki, Weronika Gonciarz, et al. "Influence of Agronomic Practice on Total Phenols, Carotenoids, Chlorophylls Content, and Biological Activities in Dry Herbs Water Macerates." Molecules 26, no. 4 (2021): 1047. http://dx.doi.org/10.3390/molecules26041047.

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Oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) have long been known for their organoleptic properties. Both plants are widely used in cuisine worldwide in fresh and dried form and as a pharmaceutical raw material. The study aimed to assess if the type of cultivation influenced chosen chemical parameters (total polyphenols by Folin-Ciocalteu method; carotenoids and chlorophyll content by Lichtenthaler method), antimicrobial activity (with chosen reference microbial strains) and shaped cytotoxicity (with L929 mouse fibroblasts cell line) in water macerates of dry oregano and thyme.

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Baranov, E. V., A. V. Buravsky, Z. B. Kvacheva, et al. "Potential of Using Cultivated Dermal Fibroblasts on the Biodegradable Polymeric Matrices for Treating Skin Damages in the Experiment." Novosti Khirurgii 25, no. 3 (2017): 223–32. http://dx.doi.org/10.18484/2305-0047.2017.3.223.

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Gaullier, Jean-Michel, Marc Gèze, René Santus, et al. "SUBCELLULAR LOCALIZATION OF AND PHOTOSENSITIZATION BY PROTOPORPHYRIN IX IN HUMAN KERATINOCYTES AND FIBROBLASTS CULTIVATED WITH 5-AMINOLEVULINIC ACID." Photochemistry and Photobiology 62, no. 1 (1995): 114–22. http://dx.doi.org/10.1111/j.1751-1097.1995.tb05247.x.

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30

Ferraris, Sara, Fernando Warchomicka, Jacopo Barberi, Andrea Cochis, Alessandro Scalia, and Silvia Spriano. "Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology." Nanomaterials 11, no. 6 (2021): 1474. http://dx.doi.org/10.3390/nano11061474.

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Nano- and micro-structuring of implantable materials constitute a promising approach to introduce mechanical contact guidance effect, drive cells colonization, as well as to prevent bacteria adhesion and biofilm aggregation, through antifouling topography. Accordingly, this paper aims to extend the application of e-beam surface texturing and nano-structuring to the beta titanium alloys, which are of great interest for biomedical implants because of the low Young modulus and the reduction of the stress shielding effect. The paper shows that surface texturing on the micro-scale (micro-grooves) i

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Koehler, Miriam I., Eliza S. Hartmann, Sabine Schluessel, et al. "Impact of Periprosthetic Fibroblast-Like Cells on Osteoclastogenesis in Co-Culture with Peripheral Blood Mononuclear Cells Varies Depending on Culture System." International Journal of Molecular Sciences 20, no. 10 (2019): 2583. http://dx.doi.org/10.3390/ijms20102583.

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Co-culture studies investigating the role of periprosthetic fibroblasts (PPFs) in inflammatory osteoclastogenesis reveal contrary results, partly showing an osteoprotective function of fibroblasts and high OPG expression in monolayer. These data disagree with molecular analyses of original periosteolytic tissues. In order to find a more reliable model, PPFs were co-cultivated with peripheral blood mononuclear cells (PBMCs) in a transwell system and compared to conventional monolayer cultures. The gene expression of key regulators of osteoclastogenesis (macrophage colony-stimulating factor (MCS

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Markvicheva, Elena, Lina Bezdetnaya, Artur Bartkowiak, et al. "Encapsulated multicellular tumor spheroids as a novel in vitro model to study small size tumors." Chemical Industry 57, no. 12 (2003): 585–88. http://dx.doi.org/10.2298/hemind0312585m.

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Presently multicellular tumor spheroids (MTS) are being widely used in various aspects of tumor biology, including studies in biology and photodynamic therapy. The cellular organization of spheroids allows the recreation of in vivo small tumors much better than all common two-dimensional in vitro models. The cell encapsulation method could be proposed as a novel technique to quickly and easily prepare a large number of spheroids with narrow size distribution within a desirable diameter range. Moreover, the proposed technique for spheroid generation using encapsulated growing tumor cells could

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MARTINS, M. L., V. N. de SOUZA, J. R. E. de MORAES, F. R. de MORAES, and A. J. da COSTA. "Comparative evaluation of the susceptibility of cultivated fishes to the natural infection with myxosporean parasites and tissue changes in the host." Revista Brasileira de Biologia 59, no. 2 (1999): 263–69. http://dx.doi.org/10.1590/s0034-71081999000200010.

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The purpose of the study was to evaluate the susceptibility of 4 important cultivated fishes to sporozoan parasites. Fishes were collected bimonthly from a pond for a period of 1 year. Myxobolus colossomatis and Henneguya piaractus were found in the internal organs and gills, respectively. The combined incidence of parasitism by both myxozoa was 97.3% in pacu (Piaractus mesopotamicus), 33.3% in hybrid tambacu (Piaractus mesopotamicus x Colossoma macropomum), 5.6% in tambaqui (Colossoma macropomum) and 0% in carp (Cyprinus carpio). Pacu was the most susceptible fish and was parasitized 79.2% in

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Kaisers, Wolfgang, Petra Boukamp, Hans-Jürgen Stark, et al. "Age, gender and UV-exposition related effects on gene expression in in vivo aged short term cultivated human dermal fibroblasts." PLOS ONE 12, no. 5 (2017): e0175657. http://dx.doi.org/10.1371/journal.pone.0175657.

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35

Pontello, D., V. Barucchello, A. Uzzau, et al. "The biotechnologies in the treatment of neuro-vascular ulcers in the elder. Cultivated autografts of skin: fibroblasts and/or cheratinocytes." BMC Geriatrics 9, Suppl 1 (2009): A8. http://dx.doi.org/10.1186/1471-2318-9-s1-a8.

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Sambale, F., S. Wagner, F. Stahl, R. R. Khaydarov, T. Scheper, and D. Bahnemann. "Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines." Journal of Nanomaterials 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/136765.

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Silver nanoparticles are widely used for many applications. In this study silver nanoparticles have been tested for their toxic effect on fibroblasts (NIH-3T3), on a human lung adenocarcinoma epithelial cell line (A-549), on PC-12-cells, a rat adrenal pheochromocytoma cell line, and on HEP-G2-cells, a human hepatocellular carcinoma cell line. The viability of the cells cultivated with different concentrations of silver was determined by the MTT assay, a photometric method to determine cell metabolism. Dose-response curves were extrapolated and IC50, total lethal concentration (TLC), and no obs

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Kober, Johanna, Alfred Gugerell, Melanie Schmid, Lars-Peter Kamolz, and Maike Keck. "Generation of a Fibrin Based Three-Layered Skin Substitute." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/170427.

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A variety of skin substitutes that restore epidermal and dermal structures are currently available on the market. However, the main focus in research and clinical application lies on dermal and epidermal substitutes whereas the development of a subcutaneous replacement (hypodermis) is often disregarded. In this study we used fibrin sealant as hydrogel scaffold to generate a three-layered skin substitute. For the hypodermal layer adipose-derived stem cells (ASCs) and mature adipocytes were embedded in the fibrin hydrogel and were combined with another fibrin clot with fibroblasts for the constr

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Hillmann, G., A. Gebert, and W. Geurtsen. "Matrix expression and proliferation of primary gingival fibroblasts in a three-dimensional cell culture model." Journal of Cell Science 112, no. 17 (1999): 2823–32. http://dx.doi.org/10.1242/jcs.112.17.2823.

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The growth of cultured primary human gingival fibroblasts and the three-dimensional arrangement of the extracellular matrix in a polyester carrier system was investigated using various histological techniques. The results were compared with monolayer cultures. Collagen types I, III, V, and VI were investigated by conventional and fluorescence microscopy, scanning and transmission electron microscopy, and confocal laser scanning microscopy. Human gingival fibroblasts were obtained from tissue biopsies of five donors and were cultivated up to 5 weeks under three-dimensional culture conditions. T

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ÜBerriegler, K. P., E. Banieghbal, and B. Krammer. "SUBCELLULAR DAMAGE KINETICS WITHIN CO-CULTIVATED WI38 and VA13-TRANSFORMED WI38 HUMAN FIBROBLASTS FOLLOWING 5-AMINOLEVULINIC ACID-INDUCED PROTOPORPHYRIN IX FORMATION." Photochemistry and Photobiology 62, no. 6 (2008): 1052–57. http://dx.doi.org/10.1111/j.1751-1097.1995.tb02408.x.

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40

Duszenko, M., M. A. Ferguson, G. S. Lamont, M. R. Rifkin, and G. A. Cross. "Cysteine eliminates the feeder cell requirement for cultivation of Trypanosoma brucei bloodstream forms in vitro." Journal of Experimental Medicine 162, no. 4 (1985): 1256–63. http://dx.doi.org/10.1084/jem.162.4.1256.

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In all previous studies, bloodstream forms of Trypanosoma brucei could be grown in vitro only when supported by a feeder layer of mammalian fibroblasts. We have axenically cultivated bloodstream T. brucei by adding L-cysteine at regular intervals and appropriate concentrations. The optimum cysteine concentration depends on cell density and is close to physiological serum levels. At concentrations greater than 24 mg/liter (2 X 10(-4) M), cysteine was acutely toxic to trypanosome concentrations of 3 X 10(7)/ml. Toxicity was prevented by addition of pyruvate or catalase, which neutralize H2O2 pro

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Sulikovska, I. O., V. V. Balatskyi, T. A. Kochubei, T. P. Ruban, N. V. Ulianchych, and O. A. Piven. "Biomin is promising matrix for developing of new generation of bone tissue grafts." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 14, no. 2 (2016): 221–26. http://dx.doi.org/10.7124/visnyk.utgis.14.2.692.

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Developing of new transplants generation or "living" grafts are extremely promising area of regenerative medicine. Such transplants are a combination of achievements of modern materials science and cell biology and consist of synthetic analogues of bone and MSK patient. The aim of our study was to analyze the biocompatibility of bioactive ceramics of different chemical composition and determine the optimal composites for further development of "living" grafts Materials and MethodsThe work was carried out with primary cell cultures using. MSCs and embryonic fibroblasts were obtained from GFP mi

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Körner, Th, F. W. Rath, and H. Schmidt. "The ability of cultivated human skin fibroblasts to store vitamin A. A contribution to the cytogenesis of the Ito cells of the liver." Experimental pathology 35, no. 4 (1988): 247–51. http://dx.doi.org/10.1016/s0232-1513(88)80095-1.

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Archuadze, Shorena, Elena Mikhailova, Elena Parovichnikova, Sergei Kulikov, and Valeri Savchenko. "IL-12 Producing Capacity of Cultivated Dendritic Cells of Aplastic Anemia Patients during Disease Manifestation and Immunosuppressive Treatment." Blood 110, no. 11 (2007): 4084. http://dx.doi.org/10.1182/blood.v110.11.4084.4084.

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Abstract Dendritic cells (DCs) play principal role in the induction of antigen-specific T-cell immune response and in the development of antigen-specific self-tolerance. Moreover, they promote either Th1 or Th2 polarization of naive lymphocytes by production of immunoregulatory cytokines (IL-12 and IL-10). AA is characterized by increased Th1/Th2 ratio and by Th1 mediated antigen-specific suppression of hematopoiesis. DCs might be responsible for the activation of T-clone in AA patients. However, functional characteristics such as IL-12 production by DC in AA patients have not been studies yet

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Zharkova, I. I., Yu M. Efremov, D. V. Bagrov, et al. "The effect of poly(3-hydroxybutyrate) modification by poly(ethylene glycol) on the viability of cells grown on the polymer films." Biomeditsinskaya Khimiya 58, no. 5 (2012): 579–91. http://dx.doi.org/10.18097/pbmc20125805579.

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A biodegradable polymer of bacterial origin, poly(3-hydroxybutyrate) (PHB), is intensively studied as biomaterial for tissue engineering. However, factors determining its biocompatibility still require better understanding. To analyze the PHB films biocompatibility, the polymer material was modified by hydrophilic polymer, poly(ethylene glycol) 300 (PEG). The blends PHB/PEG with different PEG content (10, 20, 30 and 50%) were produced by subsequent incubation in water resulted in removal of 95% PEG. The surface roughness and hydrophilicity were studied by atomic force microscopy (AFM) and cont

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Engelmann, Luca, Julia Thierauf, Natalia Koerich Laureano, et al. "Organotypic Co-Cultures as a Novel 3D Model for Head and Neck Squamous Cell Carcinoma." Cancers 12, no. 8 (2020): 2330. http://dx.doi.org/10.3390/cancers12082330.

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Background: Head and neck squamous cell carcinomas (HNSCC) are phenotypically and molecularly heterogeneous and frequently develop therapy resistance. Reliable patient-derived 3D tumor models are urgently needed to further study the complex pathogenesis of these tumors and to overcome treatment failure. Methods: We developed a three-dimensional organotypic co-culture (3D-OTC) model for HNSCC that maintains the architecture and cell composition of the individual tumor. A dermal equivalent (DE), composed of healthy human-derived fibroblasts and viscose fibers, served as a scaffold for the patien

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Berger, Tim, Nóra Szentmáry, Lorenz Latta, Berthold Seitz та Tanja Stachon. "NF-κB, iNOS, IL-6, and collagen 1 and 5 expression in healthy and keratoconus corneal fibroblasts after 0.1% riboflavin UV-A illumination". Graefe's Archive for Clinical and Experimental Ophthalmology 259, № 5 (2021): 1225–34. http://dx.doi.org/10.1007/s00417-020-05058-z.

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Abstract Purpose To analyze the effect of riboflavin UV-A illumination on mRNA and protein expression of healthy (HCFs) and keratoconus human corneal fibroblasts (KC-HCFs), concerning the inflammatory markers NF-κB, iNOS, IL-6, and collagen 1 and 5 (Col 1/Col 5). Methods Keratocytes were isolated from healthy (n = 3) and keratoconus (KC) corneas (n = 3) and were cultivated in basal medium with 5% fetal calf serum, which resulted in their transformation into human corneal fibroblasts (HCFs/KC-HCFs). Cells underwent 0.1% riboflavin UV-A illumination for 250 s (CXL). NF-κB, iNOS, IL-6, Col 1, and

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Szewczyk, Katarzyna, Wioleta Pietrzak, Katarzyna Klimek, et al. "Flavonoid and Phenolic Acids Content and In Vitro Study of the Potential Anti-Aging Properties of Eutrema japonicum (Miq.) Koidz Cultivated in Wasabi Farm Poland." International Journal of Molecular Sciences 22, no. 12 (2021): 6219. http://dx.doi.org/10.3390/ijms22126219.

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Skin aging is a natural, unavoidable, and complex process caused by oxidative stress. As a consequence, it leads to an increase in the activation of extracellular matrix disruption enzymes and DNA damage. The search for natural sources that inhibit these mechanisms can be a good approach to prevent skin aging. The purpose of our study was to evaluate the composition of flavonoids and phenolic acids in the extracts obtained from the flowers, roots, and leaves of Eutrema japonicum cultivated in Poland. Then, the resultant extracts were subjected to an assessment of antioxidant, anti-collagenase,

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Berebichez-Fridman, Roberto, Ricardo Gómez-García, Julio Granados-Montiel, et al. "The Holy Grail of Orthopedic Surgery: Mesenchymal Stem Cells—Their Current Uses and Potential Applications." Stem Cells International 2017 (2017): 1–14. http://dx.doi.org/10.1155/2017/2638305.

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Only select tissues and organs are able to spontaneously regenerate after disease or trauma, and this regenerative capacity diminishes over time. Human stem cell research explores therapeutic regenerative approaches to treat various conditions. Mesenchymal stem cells (MSCs) are derived from adult stem cells; they are multipotent and exert anti-inflammatory and immunomodulatory effects. They can differentiate into multiple cell types of the mesenchyme, for example, endothelial cells, osteoblasts, chondrocytes, fibroblasts, tenocytes, vascular smooth muscle cells, and sarcomere muscular cells. M

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Truzzi, Francesca, Anne Whittaker, Chiara Roncuzzi, Annalisa Saltari, Mitchell P. Levesque, and Giovanni Dinelli. "Microgreens: Functional Food with Antiproliferative Cancer Properties Influenced by Light." Foods 10, no. 8 (2021): 1690. http://dx.doi.org/10.3390/foods10081690.

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The anti-proliferative/pro-oxidant efficacy of green pea, soybean, radish, Red Rambo radish, and rocket microgreens, cultivated under either fluorescent lighting (predominant spectral peaks in green and orange) or combination light-emitting diode (LED, predominant spectral peak in blue) was investigated using Ewing sarcoma lines, RD-ES and A673, respectively. All aqueous microgreen extracts significantly reduced cell proliferation (cancer prevention effect) to varying extents in two-dimensional sarcoma cell cultures. The effect of the polyphenol fraction in the aqueous food matrix was unrelate

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Hwang, Yong Pil, Jae Ho Choi, Hyung Gyun Kim та ін. "Cultivated ginseng suppresses ultraviolet B–induced collagenase activation via mitogen-activated protein kinases and nuclear factor κB/activator protein-1–dependent signaling in human dermal fibroblasts". Nutrition Research 32, № 6 (2012): 428–38. http://dx.doi.org/10.1016/j.nutres.2012.04.005.

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