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Dissertations / Theses on the topic 'Cultivation of cells in vitro'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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1

Goel, Sandeep. "Identification, stem cell potential and in vitro cultivation of primitive germ cells from neonatal porcine testis." Kyoto University, 2008. http://hdl.handle.net/2433/136584.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第13857号<br>農博第1672号<br>新制||農||951(附属図書館)<br>学位論文||H20||N4324(農学部図書室)<br>UT51-2008-C773<br>京都大学大学院農学研究科応用生物科学専攻<br>(主査)教授今井裕, 教授久米新一, 教授松井徹<br>学位規則第4条第1項該当

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2

Riley, Bryan Scott. "The Suspension Cultivation of, and the use of Alternative Cell lines for the In Vitro Cultivation of, Treponema Pallidum Subspecies Pallidum." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc798117/.

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This study had two objectives: to achieve suspension cultivation of Sf1Ep cells and to develop procedures for achieving the replication of T. pallidum in those cell cultures. Sf1Ep cells have been the sole cell line used for the in vitro cultivation of T. pallidum. A study was undertaken to determine if other cell lines can support growth of T. pallidum. Rabbit skin fibroblasts (RAB-9), nude mouse ear (NME) cells, and normal rebbit testis fibroblasts (RT) were compared to Sf1Ep cells for their ability to support in vitro multiplication of T. pallidum. RAB-9 cells supported multiplication of tr

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3

Glöckner, Herma. "Characterization of a new miniaturized hollow fiber bioreactor for cultivation of cell lines and primary cells to improve cytostatic drug testing in vitro." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964222779.

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4

Chen, Hong, Stefanie Wiedmer, Sacha Hanig, Rolf Entzeroth, and Michael Kurth. "Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-127190.

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The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research. Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species. For several mammalian Eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts is still lacking. This study compares several permanent cell lines with primary fetal cells of the black rat (Rattus norvegicus) concerning the qualitative in vitro development of the rat parasite Eimer

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5

Chen, Hong, Stefanie Wiedmer, Sacha Hanig, Rolf Entzeroth, and Michael Kurth. "Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells." Hindawi, 2013. https://tud.qucosa.de/id/qucosa%3A27286.

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The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research. Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species. For several mammalian Eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts is still lacking. This study compares several permanent cell lines with primary fetal cells of the black rat (Rattus norvegicus) concerning the qualitative in vitro development of the rat parasite Eimer

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6

Fabianová, Katarína. "Monoklimatická inkubační komora - řízení koncentrace plynů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-240984.

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Cell cultures are grown in special incubators ensuring appropriate culture conditions for cell survival. The introduction of the diploma thesis is dedicated to the cultivation of cells in vitro and description of sensors, especially designated for the sensing of gas concentration. The main essence of this work is a project and realization of the monoclimatic incubation chamber for the incubation of cell cultures with the opportunity to observe them under a confocal microscope. Another part of this work is dealing with the project and application of autonomous sensing system and regulation of g

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7

Jang, Mi [Verfasser], and Andreas [Akademischer Betreuer] Manz. "Microfluidic-based 3D hepatic cell cultivation as a new in vitro tool for inflammation study / Mi Jang ; Betreuer: Andreas Manz." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2017. http://d-nb.info/1152094629/34.

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8

Ortega, Ynes Rosa 1960. "Cryptosporidium parvum isolate comparisons and improvements to in vitro cultivation." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282208.

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Cryptosporidium parvum infects numerous species of mammals, including man, and is a frequent cause of diarrhea. It can be life threatening to immunocompromised individuals. The mechanisms responsible for its pathogenesis are not completely understood. Differences between isolates originating from animal and human sources are not completely defined. Six isolates of human or bovine origin from different geographical areas were examined by various protein analysis (immunofluorescence assay, SDS-polyacrylamide gel electrophoresis, isoelectrofocusing) and nucleic acid (restriction fragment length p

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9

Bockhart, James David. "Conditioning 3D biomimetic scaffolds for the cultivation of transplantable beta cells." Thesis, University of Brighton, 2013. https://research.brighton.ac.uk/en/studentTheses/06e474c1-fbd9-49d7-89ef-55c4e571ee42.

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Islet transplantation holds vast potential as a treatment for type 1 diabetes mellitus and provides recipients with short term insulin independence. A major limitation of this treatment is the lack of donor beta (β) cells available for transplantation. Significant progress has been made with stem cell differentiation protocols; current methods have generated cell populations which possess a functioning β cell phenotype. However, these cells are not suitable for clinical transplantation. Two dimensional cell culture systems do not accurately mimic the complexity of the in vivo pancreatic enviro

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10

Hunter, Nikolas Ross. "In vitro studies on endothelial cells." Thesis, Heriot-Watt University, 1987. http://hdl.handle.net/10399/1040.

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11

Wigzell, Cathy. "Differentiation of bone cells in vitro." Thesis, University of St Andrews, 1990. http://hdl.handle.net/10023/14070.

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Osteoblastic differentiation was studied in vitro using primary cultures of bone cells derived from neonatal mouse calvaria. Using alkaline phosphatase as a marker, maintenance of the osteoblastic phenotype was found to be dependent upon the presence of ascorbic acid. No toxic effect due to ascorbic acid was seen. Insulin and dexamethasone were found to stimulate alkaline phosphatase expression, the former only in the absence of ascorbic acid. Two growth factors, epidermal growth factor and platelet-derived growth factor, were found to inhibit alkaline phosphatase expression in the presence of

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12

Xie, Liangzhi. "Stoichiometric medium design and nutritional control in fed-batch cultivation of animal cells." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/10287.

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13

Huddie, P. L. "The properties of pituitary cells in vitro." Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379245.

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14

Britto, Maria Helena Seabra Soares de. "Manutenção em linhagens de camundongos e infecção in vitro de uma cepa de Cryptosporidium sp de origem humana." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317853.

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Orientador: Ana Maria Aparecida Guaraldo<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-10T21:54:16Z (GMT). No. of bitstreams: 1 Britto_MariaHelenaSeabraSoaresde_D.pdf: 1915996 bytes, checksum: 263b368c6fc66874e6b4cc046dda0817 (MD5) Previous issue date: 2007<br>Resumo: O gênero Cryptosporidium compreende atualmente 16 espécies, que já foram encontradas parasitando mais de 150 espécies animais, incluindo o homem, no qual já foram referidas infecções causadas por sete delas. O Cryptosporidium parvum é o mais estudado porque

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15

Ferrero, Grassi Andrea Eugenio. "Fluidised bed reactors for the industrial cultivation of CHO cells producing a human gonadotropin /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10502.

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16

Rowlands, Marit-Naomi. "In vitro production of osteoclasts." Thesis, University of Liverpool, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250270.

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17

Cho, Sarah K. "Lymphohematopoietic differentiation from embryonic stem cells in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ63681.pdf.

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18

Harle, Jamie. "In vitro investigation of ultrasound interaction with cells." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400035.

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19

Graves, Stephen Ellis. "Studies on human bone-derived cells in vitro." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302849.

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20

Lapenna, Antonio. "T cells development in vitro : a minimalist approach." Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/7797.

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T lymphocytes are considered an essential and advanced component of the immune system, since these cells are able to discriminate self from non-self, start up an immune reaction and further develop into memory cells. However, therapies based on the use of patient derived newly generated T cells reinoculated into humans do not exist. This is due to difficulties in replicating the peculiar conditions required for T cell development in vitro. The systems developed so far are based on the use of animal or unrelated human thymic tissue and therefore they would not be adequate to be used in any clin

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21

Cryer, Jennifer. "Studies on ovine adipocyte precursor cells in vitro." Thesis, Cardiff University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339035.

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22

Alford, Deborah Jane. "Morphogenesis of human mammary epithelial cells in vitro." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265150.

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23

Hariharan, Krithika. "In vitro nephrogenesis from human pluripotent stem cells." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19194.

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Die Homöostase wird maßgeblich durch die Niere, bestehend aus Millionen funktioneller Untereinheiten, den Nephronen, aufrechtherhalten. Chronisch geschädigte Nephrone führen zur Entwicklung einer terminalen Nierenerkrankung (TNE). Die Erzeugung renaler Zellen aus humanen pluripotenten Stammzellen (hPSCs) stellt eine vielversprechende Strategie zur regenerativen Therapie und Behandlung von TNE dar. In der vorliegenden Arbeit wurde ein Protokoll zur Differenzierung von renalen Vorläufern (RV) aus hPSCs entwickelt, welches nephronale Zelltypen und Strukturen in vitro und ex vivo erzeugte. Eine se

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24

Chang, Po-hsun. "Attachment of Legionella pneumophila to cells in vitro." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc798334/.

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The attachment and/or penetration of animal cells by two strains of Legionella pneumophila was studied in three vertebrae cell lines in vitro . The study focused on (1) differences in attachment and penetration between the two bacterial strains (an environmental isolate, Johannesburg-2, and a clinical isolate, Chicago-8) and between the cell lines (Hep-2, WI-38 and a murine line); (2) effects of L. pneumophila on cell morphology and growth; and (3) the effects of pyruvate and six sugars or sugar derivatives (D-mannose, D-Galactose, D-Glucose, L-glucose, D-fructose, and 2-deoxy-D-glucose).

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25

Broestl, Jayne Alberta. "Effect of polycations on glomerular cells in vitro." Case Western Reserve University School of Graduate Studies / OhioLINK, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=case1056547341.

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26

Wang, Nan. "In vitro differentiation of mouse embryonic stem cells into pancreatic insulin-producing cells." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508245.

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27

Lokman, Muhammad. "Development of post-meiotic germ cells from human embryonic stem cells in vitro." Thesis, University of Sheffield, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531176.

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28

Tanzeglock, Timm. "A novel lobed Taylor-Couette bioreactor for the cultivation of shear sensitive cells and tissues /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=18047.

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29

Domogala, A. J. "The characterisation of NK cells generated in vitro from cord blood haematopoietic stem cells." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1572294/.

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Natural Killer (NK) cells are effective at targeting malignant cells without being direct effectors of graft-versus-host disease. NK cell immunotherapy has been shown to be safe in the clinic however efficacy has been inconsistent. We have previously shown that NK cells can be produced in large quantities from frozen cord blood (CB) CD34+ cells. We hypothesised that producing NK cells with optimum activation status that can proliferate and persist in vivo is fundamental for the development of a clinically relevant cellular product. Here, we assess if the cells can further respond to additional

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30

Hara, Masatake. "Mast cells cause apoptosis of cardiomyocytes and proliferation of order intramyocardial cells in vitro." Kyoto University, 2000. http://hdl.handle.net/2433/180841.

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31

Ainscough, Sarah Louise. "Improved strategies for the cultivation of human limbal epithelial (HLE) grafts." Queensland University of Technology, 2008. http://eprints.qut.edu.au/18575/.

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The limbal stem cell population is located in the limbal junctional zone between the cornea and the conjunctiva, and is responsible for maintaining the corneal epithelium. Damage to the limbal stem cell population results in a condition known as limbal stem cell deficiency (LSCD), which is characterised by conjunctivalisation of the cornea, visual impairment and persistent irritation. To treat LSCD, an alternative source of human limbal epithelial (HLE) cells must be transplanted back onto the diseased cornea. Limbal tissue grafts have had a moderate degree of success. However, autologous graf

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32

Rez, Mohammed Fayez al. "Modelling and measurement of the O2-concentration for the ex vivo cultivation of cells and tissues." Dresden TUDpress, 2007. http://deposit.d-nb.de/cgi-bin/dokserv?id=2960243&prov=M&dok_var=1&dok_ext=htm.

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33

Siu, Lai-San. "TFF2 inhibits apoptosis in human epithelial cells in vitro." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250150.

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34

Oliveira, Vanessa A. G. de. "In vitro generation of CD4âº regulatory T cells." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425944.

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35

Mellodew, Karen. "In Vitro differentiation of conditionally immortalized neuroepithelial stem cells." Thesis, King's College London (University of London), 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.497245.

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36

Saunders, Margaret. "Analysis of immune responses to transformed cells in vitro." Thesis, University of Bristol, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.481435.

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37

Stefan, Peter. "Large scale in vitro expansion of mesenchymal stem cells." Thesis, University of Sheffield, 2010. http://etheses.whiterose.ac.uk/14976/.

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Mesenchymal stem cells (MSCs) can undergo self-renewal and differentiation into a variety of mature cell types. Thus, using MSCs for tissue engineering and other medical applications holds many promising advantages over normal somatic cells. However, exactly these characteristics make MSCs more difficult to grow and control in vitro. The aim of this research project was to investigate different culture systems for their utility to expand bone marrow derived MSCs in large quantities. A large scale expansion of MSCs is especially of interest since only a small number of bone marrow derived MSCs

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38

Liu, Chao. "Identification of liver stem-like cells in human derived intrahepatic biliary epithelial cells in vitro." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967345642.

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39

Selander, Lars. "In vivo and in vitro approaches to induce beta cells from stem and progenitor cells." Doctoral thesis, Umeå : Umeå University, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-25813.

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40

Hirashima, Masanori. "Maturation of embryonic stem cells into endothelial cells in an in vitro model of vasculogenesis." Kyoto University, 1999. http://hdl.handle.net/2433/181699.

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41

Kamran, Mohammad. "The suppression by Null cells of FcM receptor synthesis by human T cells in vitro." Thesis, University of Ottawa (Canada), 1989. http://hdl.handle.net/10393/6013.

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42

Sperber, Saskia [Verfasser], and Elmar [Akademischer Betreuer] Heinzle. "Metabolic and proteomic characterization of primary hepatocytes in different in vitro cultivation conditions / Saskia Sperber ; Betreuer: Elmar Heinzle." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2016. http://d-nb.info/1122438354/34.

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43

Haileselassie, Yeneneh. "Lactobacilli- and Staphylococcus aureus mediated modulation of immune responses in vitro." Doctoral thesis, Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-127399.

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The human gut harbors a vast number of microbes. These microbes are not passive bystanders. They are important in modulating the immune system. We have previously shown that early colonization with lactobacilli and Staphylococcus (S.) aureus differentially associates with allergy development and/or immune profile at early ages. Here we focus on understanding how these microbes modulate the response of intestinal epithelial cells and immune cells in vitro. In paper I, we investigated the impact of UV-killed and/or cell free supernatant (CFS) of different Lactobacillus (L.) species and S. aureus

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44

Egger, Dominik [Verfasser]. "Concepts for the implementation of physiologic conditions for the cultivation of human mesenchymal stem cells / Dominik Egger." Hannover : Technische Informationsbibliothek (TIB), 2017. http://d-nb.info/1145160077/34.

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45

Langley, Ruth E. "Radiation-induced apoptosis : in vitro studies." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387684.

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46

Moser, Stephanie. "In vitro effects of psychopharmaceuticals on peripheral mononuclear blood cells." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-144429.

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47

Shah, Hetal. "Assessment of invasive activities of ovarian cancer cells in vitro." [Tampa, Fla.] : University of South Florida, 2005. http://purl.fcla.edu/fcla/etd/SFE0001126.

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48

Hinds, Heather C. "Evaluating terminal differentiation of porcine valvular interstitial cells in vitro." Link to electronic thesis, 2006. http://www.wpi.edu/Pubs/ETD/Available/etd-050506-113014/.

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49

Verstijnen, Cornelis Petrus Henricus Josephus. "In vivo and in vitro characterization of colorectal cancer cells." Maastricht : Maastricht : Rijksuniversiteit Limburg ; University Library, Maastricht University [Host], 1987. http://arno.unimaas.nl/show.cgi?fid=5372.

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50

Keen, Leigh John. "The growth and pharmacology of insect glial cells in vitro." Thesis, Oxford Brookes University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359837.

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