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Journal articles on the topic 'Cultivation of cells in vitro'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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1

Popović, Maja, Mirna Tominac, Ksenija Vlahović, et al. "In vitro cultivation of porcine limbal stem cells." Italian Journal of Animal Science 8, sup3 (2009): 125–27. http://dx.doi.org/10.4081/ijas.2009.s3.125.

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2

Ward, Rachel. "Book Review: in vitro Cultivation of Animal Cells." Alternatives to Laboratory Animals 23, no. 1 (1995): 161–62. http://dx.doi.org/10.1177/026119299502300122.

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3

Liu, Yan, Yan Hui Gong, Qing Ren Zeng, et al. "In vitro cultivation of schistosoma japonicum germinal cells." Cell Biology International 32, no. 3 (2008): S60. http://dx.doi.org/10.1016/j.cellbi.2008.01.255.

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4

Sládek, Z., D. Vašíčková, and D. Ryšánek. "The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils." Veterinární Medicína 47, No. 12 (2012): 325–32. http://dx.doi.org/10.17221/5843-vetmed.

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The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop&shy;tosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophi

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5

Pilinska, M., O. Shemetun, O. Talan, O. Dibska, S. Kravchenko, and V. Sholoiko. "STUDY THE EFFECTS OF IONIZING RADIATION ON THE LEVEL OF CHROMOSOME INSTABILITY IN HUMAN SOMATIC CELLS DURING THE DEVELOPMENT OF TUMOR-INDUCED BYSTANDER EFFECT." Проблеми радіаційної медицини та радіобіології = Problems of Radiation Medicine and Radiobiology 25 (2020): 353–61. http://dx.doi.org/10.33145/2304-8336-2020-25-353-361.

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Objective. to determine the impact of the irradiated in vitro blood cells from patients with B-cell chronic lymphocytic leukemia (CLL) on the level of chromosomal instability in peripheral blood lymphocytes (PBL) from healthy persons during the development of tumor-induced bystander effect. Materials and methods. Separate and joint cultivation of PBL from healthy persons (cells-bystanders) together with blood cells from CLL patients irradiated in vitro at the G0 stage of the mitotic cycle by γ-quanta 137Cs in a dose of 0.5 Gy 137Cs (cells-inductors) was used. For joint cultivation our own mode

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6

Bayne, Christopher J., Jane S. Menino, Deborah J. Hobbs, and David W. Barnes. "In vitro Cultivation of Cells from Larval Schistosoma mansoni." Journal of Parasitology 80, no. 1 (1994): 29. http://dx.doi.org/10.2307/3283341.

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7

Ye, Qing, Hui-Fen Dong, Christoph G. Grevelding, and Min Hu. "In vitro cultivation of Schistosoma japonicum-parasites and cells." Biotechnology Advances 31, no. 8 (2013): 1722–37. http://dx.doi.org/10.1016/j.biotechadv.2013.09.003.

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8

Hajdu, Steven I. "The first cultivation of malignant tumor cells in vitro." Journal of the American Society of Cytopathology 5, no. 1 (2016): 1–2. http://dx.doi.org/10.1016/j.jasc.2015.11.005.

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9

BURNSTEIN, THEODORE, JAN RHODES, and JOHN TUREK. "Adherenceof Pneumocystis cariniiin Lung Cells during in Vitro Cultivation." Journal of Protozoology 36, no. 1 (1989): 35S—37S. http://dx.doi.org/10.1111/j.1550-7408.1989.tb02684.x.

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10

BURNSTEIN, THEODORE, JAN RHODES, and JOHN TUREK. "Adherence ofPneumocystis cariniiin Lung Cells during in Vitro Cultivation." Journal of Protozoology 36 (May 1989): 35s—37s. http://dx.doi.org/10.1111/j.1550-7408.1989.tb05821.x.

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11

Trišić, Dijana, Vukoman Jokanović, Đorđe Antonijević, and Dejan Marković. "Stem cells in tissue engineering – dynamic cultivation requirement." Stomatoloski glasnik Srbije 65, no. 1 (2018): 37–44. http://dx.doi.org/10.2478/sdj-2018-0005.

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Summary Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells’ niche from in vivo conditions. In an attempt to pro

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12

Johnson, Mark D., George T. Bryan, and Catherine A. Reznikoff. "Serial Cultivation of Normal Rat Bladder Epithelial Cells in Vitro." Journal of Urology 133, no. 6 (1985): 1076–81. http://dx.doi.org/10.1016/s0022-5347(17)49383-9.

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13

Levy, M., D. Meuten, and E. Breitschwerdt. "Cultivation of Rhinosporidium seeberi in vitro: interaction with epithelial cells." Science 234, no. 4775 (1986): 474–76. http://dx.doi.org/10.1126/science.3764422.

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14

TEGOSHI, TATSUYA, and YUKIO YOSHIDA. "New System of in Vitro Cultivation ofPneumocystis cariniiwithout Feeder Cells." Journal of Protozoology 36 (May 1989): 29s—31s. http://dx.doi.org/10.1111/j.1550-7408.1989.tb05818.x.

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15

Syrvatka, V. J., Yu I. Slyvchuk, O. V. Shtapenko, O. M. Gromyko, and I. I. Gevkan. "Combined space-organized system for animals oocytes maturation in vitro." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 264–69. http://dx.doi.org/10.7124/feeo.v26.1277.

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Aim. The aim of the work was to create a space-organized system for oocytes maturation based on rabbits granulosa cells, hyaluronic acid (for structural organization of this system) and insulin, as an important growth factor. Methods. Cultivation of granulosa cells and oocytes maturation in vitro, biochemical determination of lactate dehydrogenase activity and cholesterol concentration, and determination of progesterone and estradiol concentrations by enzyme immunoassay were done. Results. Hyaluronic acid in concentration of 0.5 μg/ml increased proliferation of granulosa cells and progesterone

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16

Brůčková, Lenka, Tomáš Soukup, Jiří Moos, et al. "The Cultivation of Human Granulosa Cells." Acta Medica (Hradec Kralove, Czech Republic) 51, no. 3 (2008): 165–72. http://dx.doi.org/10.14712/18059694.2017.19.

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The major functions of granulosa cells (GCs) include the production of steroids, as well as a myriad of growth factors to interact with the oocyte during its development within the ovarian follicle. Also FSH stimulates GCs to convert androgens (coming from the thecal cells) to estradiol by aromatase. However, after ovulation the GCs produce progesterone that may maintain a potential pregnancy. Experiments with human GCs are mainly focused on the purification of GCs from ovarian follicular fluid followed by FACS analysis or short-term cultivation. The aim of our study was to cultivate GCs for a

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17

Schuster, Frederick L. "Cultivation of Plasmodium spp." Clinical Microbiology Reviews 15, no. 3 (2002): 355–64. http://dx.doi.org/10.1128/cmr.15.3.355-364.2002.

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SUMMARY Cultivation of both human and non-human species of Plasmodium spp., the causal agent of malaria, has been a major research success, leading to a greater understanding of the parasite. Efforts at cultivating the organisms in vitro are complicated by the parasites' alternating between a human host and an arthropod vector, each having its own set of physiological, metabolic, and nutritional parameters. Life cycle stages of the four species that infect humans have been established in vitro. Of these four, P. falciparum remains the only species for which all stages have been cultured in vit

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18

Morakote, N., and K. Charuchinda. "Storage of red cells for in vitro cultivation of Plasmodium falciparum." Annals of Tropical Medicine & Parasitology 82, no. 6 (1988): 625–26. http://dx.doi.org/10.1080/00034983.1988.11812299.

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19

Li, Wei, Junjie Zeng, Ganghua Zhu, Yunpeng Dong, Dinghua Xie, and Ruosha Lai. "In Vitro Cultivation Technology of Rat Bone Marrow Mesenchymal Stem Cells." Journal of Biomaterials and Tissue Engineering 9, no. 1 (2019): 62–68. http://dx.doi.org/10.1166/jbt.2019.1946.

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20

Emura, Makito. "Stem cells of the respiratory epithelium and their in vitro cultivation." In Vitro Cellular & Developmental Biology - Animal 33, no. 1 (1997): 3–14. http://dx.doi.org/10.1007/s11626-997-0015-4.

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21

Čolić, Miodrag. "Cultivation, characterization and modulation of rat thymic nonlymphoid cells in vitro." Cytotechnology 5, no. 2 (1991): 107–15. http://dx.doi.org/10.1007/bf00365427.

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22

Gaiba, Silvana, Lucimar Pereira de França, Jerônimo Pereira de França, and Lydia Masako Ferreira. "Characterization of human adipose-derived stem cells." Acta Cirurgica Brasileira 27, no. 7 (2012): 471–76. http://dx.doi.org/10.1590/s0102-86502012000700007.

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PURPOSE: There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs. METHODS: Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed. R

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23

Liubich, L., M. Lisyany, T. Malysheva, V. Semenova, L. Staino та V. Vaslovich. "Morphometric characteristics of TGF-β1-positive cells of fetal rat brain in vitro". Cell and Organ Transplantology 4, № 2 (2016): 211–15. http://dx.doi.org/10.22494/cot.v4i2.58.

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One of the directions of cell therapy being developed for brain gliomas is the use of the neurogenic stem and progenitor cells (NSCs/NPCs). There are data on the anti-tumor and immunomodulating properties of the NSCs/NPCs the mechanisms of which were not disclosed yet. One of the potential targets for tumor therapy is the transforming growth factor β (TGF-β1) which is thought to be one of the key molecules in the regulation of proliferation, differentiation and cell survival or apoptosis. In the view of available information about the possibility of TGF-β1 production by the mammalian multipote

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24

Kladnytska, L. V., A. I. Mazurkevych, V. T. Khomych, et al. "Morphological peculiarites and functional activity of adipose-derived mesenchimal stem cells during in vitro cultivation conditions." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 92 (2018): 79–82. http://dx.doi.org/10.32718/nvlvet9216.

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The studies were conducted on 2-3-months-old males of C57BL/6 mice weighing 20–24 g. Obtaining and cultivating of adipose-derived mesenchimal stem cells (AD MSCs) were carried out in a sterile laminar box with compliance of conditions of asepsis and antiseptics. AD MSCs of the 2, 4, 7 and 12 passages were analyzed. Morphometric analysis was performed using a light microscopy. Morphometric parameters such as cell and nucleus area or nuclear-cytoplasmic ratio (NCR) were calculated using the Axiovision light microscope (Carl Zeiss, Germany) and ImageJ 1.45 software. Trypan blue dye used for inves

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25

Petrakova, O. S., V. V. Ashapkin, E. A. Voroteliak, et al. "Effect of 3D Cultivation Conditions on the Differentiation of Endodermal Cells." Acta Naturae 4, no. 4 (2012): 47–57. http://dx.doi.org/10.32607/20758251-2012-4-4-47-57.

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Cellular therapy of endodermal organs is one of the most important issues in modern cellular biology and biotechnology. One of the most promising directions in this field is the study of the transdifferentiation abilities of cells within the same germ layer. A method for an in vitro investigation of the cell differentiation potential (the cell culture in a three-dimensional matrix) is described in this article. Cell cultures of postnatal salivary gland cells and postnatal liver progenitor cells were obtained; their comparative analysis under 2D and 3D cultivation conditions was carried out. Bo

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26

Yang, Chao, Liang Sun, Xinghan Li, et al. "The Potential of Dental Stem Cells Differentiating into Neurogenic Cell Lineage after Cultivation in Different ModesIn Vitro." Cellular Reprogramming 16, no. 5 (2014): 379–91. http://dx.doi.org/10.1089/cell.2014.0026.

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27

Mäkitie, Antti A., Yongnian Yan, Xiaohong Wang, et al. "In Vitro Evaluation of a 3D PLGA–TCP Composite Scaffold in an Experimental Bioreactor." Journal of Bioactive and Compatible Polymers 24, no. 1_suppl (2009): 75–83. http://dx.doi.org/10.1177/0883911508101745.

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A 3D poly(lactic acid-co-glycolic acid)/tricalcium phosphate (PLGA-TCP) composite scaffold, generated with the low-temperature deposition modeling rapid prototyping technique, was tested for its viability in a 3D cell cultivation in vitro. The aim was to find optimal cell culture conditions for the selected scaffold material and to monitor cell division, differentiation, and migration of selected cell types in this environment. In addition, the behavior and cell-matrix interactions of selected cell types were monitored as well as the biodegradation rate of the tested scaffold material. Chinese

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28

Nikolits, Ilias, Sabrina Nebel, Dominik Egger, Sebastian Kreß, and Cornelia Kasper. "Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells." Cells 10, no. 4 (2021): 886. http://dx.doi.org/10.3390/cells10040886.

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Mesenchymal stem cells (MSCs) are of great interest for their use in cell-based therapies due to their multipotent differentiation and immunomodulatory capacities. In consequence of limited numbers following their isolation from the donor tissue, MSCs require extensive expansion performed in traditional 2D cell culture setups to reach adequate amounts for therapeutic use. However, prolonged culture of MSCs in vitro has been shown to decrease their differentiation potential and alter their immunomodulatory properties. For that reason, preservation of these physiological characteristics of MSCs

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29

Kanavi, MozhganRezaei, Narges Fazili, Sahar Balagholi, Yashar Amizadeh, and SeyedBagher Hosseini. "Cultivation of retinoblastoma cells: Correlation between in vitro growth pattern and histopathology." Journal of Ophthalmic and Vision Research 11, no. 4 (2016): 379. http://dx.doi.org/10.4103/2008-322x.194086.

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30

Storck, K., J. Ell, S. Regn, et al. "Optimization of in vitro cultivation strategies for human adipocyte derived stem cells." Adipocyte 4, no. 3 (2015): 181–87. http://dx.doi.org/10.4161/21623945.2014.987580.

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31

FRANZEN, CASPAR, SUSANNE FISCHER, JOSEF SCHROEDER, et al. "In Vitro Cultivation of an Insect Microsporidian Tubulinosema ratisbonensis in Mammalian Cells." Journal of Eukaryotic Microbiology 52, no. 4 (2005): 349–55. http://dx.doi.org/10.1111/j.1550-7408.2005.00043x.

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32

FENG, Ren Qing, Li Ying DU, and Zhen Quan GUO. "In vitro cultivation and differentiation of fetal liver stem cells from mice." Cell Research 15, no. 5 (2005): 401–5. http://dx.doi.org/10.1038/sj.cr.7290308.

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33

Bammert, Tyler D., Collin A. Beckstrom, Grace Lincenberg, Jamie G. Hijmans, Jared J. Greiner, and Natalia G. Rocha. "Phenotypic differences in early outgrowth angiogenic cells based on in vitro cultivation." Cytotechnology 71, no. 2 (2019): 665–70. http://dx.doi.org/10.1007/s10616-019-00305-6.

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34

Rapoport, Daniel H., Simone Schicktanz, Emel Gürleyik, Christine Zühlke, and Charli Kruse. "Isolation and in vitro cultivation turns cells from exocrine human pancreas into multipotent stem-cells." Annals of Anatomy - Anatomischer Anzeiger 191, no. 5 (2009): 446–58. http://dx.doi.org/10.1016/j.aanat.2009.07.002.

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35

Szczotka-Bochniarz, Anna, Katarzyna Podgórska, Agnieszka Nowak, and Zygmunt Pejsak. "In vitro cultivation and immunostaining of Lawsonia intracellularis strains." Bulletin of the Veterinary Institute in Pulawy 57, no. 3 (2013): 319–22. http://dx.doi.org/10.2478/bvip-2013-0055.

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Abstract The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was also performed. Large number of S-shaped, moving bacteria were found in the cell medium in cultures infected with ATCC 55783 and vaccine strain. Immunostaining revealed a high number of multiple cell-associated or intracellular red stained bacteria in the infected cultures. This study describe

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36

Strauss, P. G., E. I. Closs, J. Schmidt, and V. Erfle. "Gene expression during osteogenic differentiation in mandibular condyles in vitro." Journal of Cell Biology 110, no. 4 (1990): 1369–78. http://dx.doi.org/10.1083/jcb.110.4.1369.

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The cartilagenous tissue of mandibular condyles of newborn mice contains progenitor cells as well as young and mature chondrogenic cells. During in vitro cultivation of the tissue, progenitor cells undergo osteogenic differentiation and form new bone (Silbermann, M., D. Lewinson, H. Gonen, M. A. Lizarbe, and K. von der Mark. 1983. Anat. Rec. 206:373-383). We have studied the expression of genes that typify osteogenic differentiation in mandibular condyles during in vitro cultivation. RNAs of the genes for collagen type I, osteonectin, alkaline phosphatase, and bone gla protein were sequentiall

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37

Siller, Ina G., Niklas-Maximilian Epping, Antonina Lavrentieva, Thomas Scheper, and Janina Bahnemann. "Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis." Materials 13, no. 19 (2020): 4290. http://dx.doi.org/10.3390/ma13194290.

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Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish—an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirect

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Zhdanova, Natalya S., Evgenia A. Vaskova, Tatyana V. Karamysheva, Julia M. Minina, Nicolay Rubtsov, and Suren M. Zakian. "Dysfunction telomeres in embryonic fibroblasts and cultured in vitro pluripotent stem cells of Rattus norvegicus (Rodentia, Muridae)." Comparative Cytogenetics 13, no. 3 (2019): 197–210. http://dx.doi.org/10.3897/compcytogen.v13i3.34732.

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We studied the level of spontaneous telomere dysfunction in Rattus norvegicus (Berkenhout, 1769) (Rodentia, Muridae) embryonic fibroblasts (rEFs) and in cultured in vitro rat pluripotent stem cells (rPSCs), embryonic stem cells (rESCs) and induced pluripotent stem cells (riPSCs), on early passages and after prolonged cultivation. Among studied cell lines, rESCs showed the lowest level of telomere dysfunction, while the riPSCs demonstrated an elevated level on early passages of cultivation. In cultivation, the frequency of dysfunctional telomeres has increased in all studied cell lines; this is

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39

Rybachuk, O., V. Кyryk, P. Poberezhny, G. Butenko, G. Skibo, and T. Pivneva. "Effect of the bone marrow multipotent mesenchimal stromal cells to the neural tissue after ischemic injury in vitro." Cell and Organ Transplantology 2, no. 1 (2014): 74–78. http://dx.doi.org/10.22494/cot.v2i1.38.

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Stem cells application in neural system injuries is an actual and prospective scientific field of modern regenerative medicine. In recent years much attention has been paid for study of regenerative effects of multipotent mesenchymal stromal cells (MMSCs) from different sources on injured tissues.The aim of our study was to determine the level of tissue damage in hippocampus after in vitro model of ischemia and to investigate the effect of bone marrow MMSСs in non-contact co-culture with ischemic neural tissue. The ischemic injury of neural tissue in vitro was modeling in organotypic hippocamp

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40

Szydlak, Renata, Marcin Majka, Małgorzata Lekka, Marta Kot, and Piotr Laidler. "AFM-based Analysis of Wharton’s Jelly Mesenchymal Stem Cells." International Journal of Molecular Sciences 20, no. 18 (2019): 4351. http://dx.doi.org/10.3390/ijms20184351.

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Wharton’s jelly mesenchymal stem cells (WJ-MSCs) are multipotent stem cells that can be used in regenerative medicine. However, to reach the high therapeutic efficacy of WJ-MSCs, it is necessary to obtain a large amount of MSCs, which requires their extensive in vitro culturing. Numerous studies have shown that in vitro expansion of MSCs can lead to changes in cell behavior; cells lose their ability to proliferate, differentiate and migrate. One of the important measures of cells’ migration potential is their elasticity, determined by atomic force microscopy (AFM) and quantified by Young’s mod

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Walum, Erik, Elisabeth Hansson, and Alan L. Harvey. "In Vitro Testing of Neurotoxicity." Alternatives to Laboratory Animals 18, no. 1_part_1 (1990): 153–79. http://dx.doi.org/10.1177/026119299001800118.1.

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Many of the toxic compounds that are at large in the environment represent a risk to our neuronal functions. Chemicals may have a direct or indirect effect on the nervous system and they may interfere with general biochemical properties or specific neuronal structures and processes. In this review, a brief presentation of the major neurotoxicological targets is given, together with a discussion of some aspects of the use of different in vitro models for screening purposes and mechanistic studies. It is believed that in vitro methods offer special opportunities for the development of new neurot

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42

Arnold, U. "In vitro-cultivation of human periosteum derived cells in bioresorbable polymer-TCP-composites." Biomaterials 23, no. 11 (2002): 2303–10. http://dx.doi.org/10.1016/s0142-9612(01)00364-7.

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43

Friedenstein, A. J., R. K. Chailakhyan, and U. V. Gerasimov. "Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers." Cell Proliferation 20, no. 3 (1987): 263–72. http://dx.doi.org/10.1111/j.1365-2184.1987.tb01309.x.

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44

Bobek, V., R. Lischke, J. Schutzner, and K. Kolostova. "P-138 * CIRCULATING TUMOUR CELLS IN THORACIC MALIGNANCIES: SEPARATION AND CULTIVATION IN VITRO." Interactive CardioVascular and Thoracic Surgery 18, suppl 1 (2014): S36—S37. http://dx.doi.org/10.1093/icvts/ivu167.138.

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45

Khanova, M. Yu, E. A. Velikanova, T. V. Glushkova, and V. G. Matveeva. "Development of personalized cell-populated vascular graft in vitro." Complex Issues of Cardiovascular Diseases 10, no. 2 (2021): 89–93. http://dx.doi.org/10.17802/2306-1278-2021-10-2s-89-93.

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Aim. To create a personalized cell-populated small-diameter vascular prosthesis in a pulsating bioreactor.Methods. Tubular grafts were made by electrospinning from mixtures of biodegradable polymers, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(εcaprolactone) (PCL). The inner surface is modified with fibrin. Tubular scaffolds were colonized with cultured colony-forming endothelial cells and grown under static conditions for 2 days. Then, the cell-populated prostheses continued to be cultivated for 5 days in a pulsating bioreactor system with a final shear stress of 2.85 dynes/

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46

Kovalenko, O. Hr, A. M. Kyrychenko, and O. Yu Kovalenko. "Virus Infected Bean Tissue Culture Cells and It’s Healing in vitro Using Liposomal form of Glycanes." Mikrobiolohichnyi Zhurnal 82, no. 5 (2020): 58–64. http://dx.doi.org/10.15407/microbiolj82.05.058.

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The aim of the study was to develop a recovery means for beans infected by Bean yellow mosaic virus (BYMV) as well as Bean common mosaic virus (BCMV) using callus culture and liposomal glycan preparations. Methods. Cultivation of explants and callus cultures was carried out in vitro using conventional methods of plant biotechnology. The tissue culture propagation was performed during the spring or summer seasons. The presence of viral infection was tested by reverse transcription polymerase chain reaction. The virus-specific primers that allowed amplifying the conserved regions of the capsid p

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47

Khochenkova, Yu A., I. G. Dyrda, Yu S. Machkova, et al. "New approaches in 3D modeling of in vitro growth of primary cultures of malignant gliomas." Advances in molecular oncology 6, no. 4 (2019): 69–74. http://dx.doi.org/10.17650/2313-805x-2019-6-4-69-74.

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Background. The incidence of brain gliomas firmly occupies a leading position among all central nervous system tumors – 40–50 % of the cases detected, more than half of them are glioblastoma. Existing cell lines and cultivation methods do not reflect all the features of the three-dimensional (3D) organization of native glioblastoma. The use of temozolomide leads to the development of drug resistance and acute relapse, followed by a poor clinical outcome. The development of resistance is largely associated with the presence of tumor stem cells in the population and intratumoral heterogeneity. O

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Aizenshtadt, A. A., M. A. Skazina, E. A. Kotelevskaya, et al. "Charachterization of umbilical cord mesenchymal stromal cells during long-term expansion in vitro." HERALD of North-Western State Medical University named after I.I. Mechnikov 10, no. 1 (2018): 11–19. http://dx.doi.org/10.17816/mechnikov201810111-19.

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One of the clinicians’ major concerns is the biological safety of MSC. The critical question for clinical application of human MSC is their ability to undergo spontaneous malignant transformation in a recipient organism. The goal of our research was to study umbilical cord hMSC proliferative and differentiation capacities, karyotype stability, telomerase activity and telomere length, oncomarkers expression and tumorigenicity during long-term (6 months) cultivation ex vivo. Here we report on the establishing the primary culture of human umbilical cord MSC, MSC_0714, that was capable to prolifer

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Nguyen, The Duy, Darius Widera, Johannes Greiner, et al. "Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells." Biological Chemistry 394, no. 12 (2013): 1623–36. http://dx.doi.org/10.1515/hsz-2013-0191.

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Abstract Neural precursor cells (NPCs) are lineage-restricted neural stem cells with limited self-renewal, giving rise to a broad range of neural cell types such as neurons, astrocytes, and oligodendrocytes. Despite this developmental potential, the differentiation capacity of NPCs has been controversially discussed concerning the trespassing lineage boundaries, for instance resulting in hematopoietic competence. Assessing their in vitro plasticity, we isolated nestin+/Sox2+, NPCs from the adult murine hippocampus. In vitro-expanded adult NPCs were able to form neurospheres, self-renew, and di

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Cawthorn, R. J., R. J. F. Markham, N. D. Hitt, and D. Despres. "In vitro cultivation of the vascular phase of Sarcocystis hirsuta (Apicomplexa)." Canadian Journal of Zoology 68, no. 5 (1990): 1068–70. http://dx.doi.org/10.1139/z90-157.

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Sporozoites of Sarcocystis hirsuta (Apicomplexa), although they also penetrated into bovine monocytes, developed to the schizogonous phase only in bovine pulmonary artery endothelial cells. Schizonts were evident beginning 14 days after sporozoite inoculation (DAI) and persisted to 62 DAI, when experiments were terminated. Merozoites and schizonts were most numerous 35–53 DAI. The number of schizogonous generations was not determined. In vitro cultivation of schizonts of S. hirsuta will facilitate comparisons with development of S. cruzi, and this will aid elucidation of mechanisms of pathogen

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