Dissertations / Theses on the topic 'Cultural genesis'

Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2014.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (page 108).<br>The original question was: "Can machines think?" Alan Turing asked: "Does there exist a digital computer that can do sufficiently well at the imitation game?" Patrick Winston asked: "What makes human intelligence different from that of other primates?" Winston's answer came in the form of four hypotheses that are the core behind the vision of the Genesis group at MIT's Computer Science and Artificial Intelligence Laboratory, which has developed the Genesis story understanding system. The key focus behind this system is: stories are an essential component of what makes human intelligence so remarkably different from that of other animals. I believe that if Winston and the Genesis group are correct and stories are a key part of human intelligence, then it is necessary that Genesis, the system that serves to demonstrate this point, be capable of handling stories from all cultures, including less well-known cultures such as that of the Crow indians, a tribe from the northern plains of the United States. Over the course of my work, I analyzed three collections of Crow literature, created a list of cultural features present in the stories, identified four as particularly important (unknowable events, medicine, differences as strengths, and uniform treatment of entities), and developed a set of five Genesis-readable stories in which those four features were prominent. This led to several new elements in the story understanding model; with these new elements, Genesis is capable of understanding stories from the Crow culture, bringing it one step closer to being a universal story understanding system.<br>by Wolfgang Victor Hayden Yarlott.<br>M. Eng.

This thesis aims to be a critical investigation into the representation of bullfighting in the history of Spanish cinema, through the framework of Antonio Gramsci’s concept of cultural hegemony (Gramsci [1929-1935] 2007), including the concept of ‘orientalism’ (Said 1978), and the study of identity in film genre and national cinema. I explore how some dominant cultural elements, such as male virility, ultra-conservative Catholicism and political nationalism, exerted their influence on films about bullfighting throughout history. With a close textual analysis of an extended filmic corpus of cine taurino, which includes fictions and documentaries, popular films and cinéma d’essai, and comparative studies with other national cinemas, I investigate which predominant meanings have been conveyed through visual representations of bulls, bullfighters and bullrings, and how these meanings can find their roots in bullfighting culture itself and in cultural movements of 20th Century Spain. As a brand-new study in the field of Spanish Film Studies, with little literature available, the investigation explores all the three major eras of Spanish film history, which correspond to specific political situations in the Spanish state: - The pre-Franco era (1896-1939), focused on the genesis of the cinematic genre and subgenre and on the controversies about the macro-genre of the filmic españolada in silent films (Navarrete Cardero 2009); - The Francoist dictatorship (1939-1975), focused on the political evolution of bullfighting representation in national cinema as a metaphor for class struggle; - Post-Franco cinema (1975 - 2012), focused on the new wave of filmmakers who portrayed gender reversal in virility, bravery and strength (now conferred to the literal or symbolic matadora), using formal elements of the stereotypical representation of Spanishness.

Natural soils in the Appalachian coal mining region occur on steep slopes and are often thin, rocky, acidic, and/or infertile. This often makes use of natural topsoil for surface coal mine reclamation impractical. Sandstone (SS) and siltstone (SiS) topsoil substitute materials in mixed and pure treatments were compared to ascertain their effects on soil genesis and tall fescue (Festuca arundinaceae Schrab.) growth in a rock mix study. A second surface amendment experiment with similar objectives was established and contained treatments of sawdust, topsoil, a control, and four rates of sewage sludge. Soil pH, extractable P, and coarse fragment contents decreased at the soil surface from 1982 to 1984. The CEC decreased from 1982 to 1983 due to leaching of cations solubilized from carbonates, but stabilized by 1984. Soil total-N and water availability increased from 1982 to 1984. Particle size distributions changed as sand and >2 mm sized materials weathered into silt and clay sized particles. As sand contents decreased over time, silt contents increased. Morphologically distinct A horizons developed in these soils rapidly and were formed primarily by plant rooting and organic matter additions. Treatment application, topsoil substitute placement, and grading also created morphologically distinct horizons. Fescue biomass production indicates topsoil substitutes support more vigorous vegetative growth than topsoil, especially when topsoils are mixtures of subsoil and A horizons. Biomass production was primarily limited by N, P, and water availability. Blended rock mixes consistently supported higher biomass production than pure SS and SiS treatments. Municipal sewage sludge applied at rates-56 Mg/ha equaled or exceeded inorganic fertilization for establishment and maintenance of tall fescue stands, and did not cause heavy metal phytotoxicity.<br>M.S.

Despite the dynamic portrayal of clothes in the Hebrew Bible scholars continue to interpret them as flat and inert objects. They are often overlooked or reduced to background details in the biblical texts. However, this thesis will demonstrate that the biblical writers’ depictions of clothes are not incidental and should not be reduced to such depictions. This thesis employs a multidisciplinary approach to develop and challenge existing approaches to the clothing imagery in the Hebrew Bible. It will fall into two main parts. In the first part, I draw insights from material-cultural theories to reconfigure ways of thinking about clothing as material objects, and reassessing the relationships between people and objects. Having challenged some of the broader conceptions of clothing, I will turn to interrogate the material and visual evidence for clothing and textiles from ancient Syro- Palestinian and ancient West Asian cultures to construct a perspective of the social and material impact of clothing in the culture in which the biblical texts were constructed and formed. In the second part, I will examine the biblical writers’ depiction of clothing through two case studies: Joseph’s ketonet passim (Genesis 37) and Elijah’s adderet (1 Kings 19 and 2 Kings 2). These analyses will draw from the insights made in the first part of this thesis to reassess and challenge the conventional scholarly interpretations of clothing in these texts. In this thesis, I argue that clothes are employed in powerful ways as material objects which construct and develop the social, religious and material dimensions of the text. They are also intimately entangled in relationships with the characters portrayed by the biblical writers and can even be considered as extensions of the people with whom they are engaged. Clothes manifest their own agency and power, which can transform other persons and objects through their performance and movement in a biblical text.

Thesis (MSc)--University of Stellenbosch, 2008.<br>ENGLISH ABSTRACT: The five leading abalone producers in South Africa have initiated a genetic enhancement program for Haliotis midae in a collaborative effort to improve economically valuable traits. Several independent objective-specific studies were initiated, including the establishment of a Performance Recording Scheme (PRS), utilised in this study, and necessary to monitor the ongoing performance of individuals as the move from mass-selection to marker assisted selection (MAS) is implemented. The primary objective of this study was parentage assignment of F1 offspring mass-selected for size at approximately one year and allocated to either a “faster” or a “slower” growth group. Nine microsatellite markers were used to genotype juveniles and potential parents, with assignment completed using CERVUS 2.0. Average growth results for Abagold and HIK were comparable for both growth groups. Slight environmental effects, although not statistically significant, were evident as growth advantages for juveniles within the faster growth group at two of the five locations and for juveniles within the slower growth group at one of the five rearing locations. Despite measures to standardise environmental influences, variables are difficult to control within the reality of a production environment; and potential genotype x environment interactions may require further investigation and factoring into future breeding programs. The additional costs associated with MAS often make the technology prohibitive to most aquaculture operations, despite the significant genetic gains to be realised from its implementation. Cost-optimising routine processes such as DNA extractions may be one approach to reduce these additional costs. Chelex®100 appears to be a suitable alternative to the CTAB method – being quick and cost-effective to perform. Applying this method in combination with the high throughput of a robotic platform warrants further evaluation. For the microsatellite development, 50% of positive recombinant clones contained inserts. Sequencing of these clones produced 16% perfect repeats and 47% imperfect repeats for which 52 primer sets were designed and tested. In total, 31 polymorphic microsatellite loci of different motifs and composition were developed. Sixty-one percent of sequenced clones were deemed redundant and pre-screening for both uniqueness and the presence of microsatellites would reduce unnecessary sequencing thus improving the efficiency of the FIASCO method and reducing costs. Nine loci were selected for parentage assignments. Null alleles were present for all the selected markers; however, frequencies were below the critical level of 5%. Parentage yielded 91% and 90% successful assignment for Abagold and HIK respectively; however, observations indicate that a measure of relatedness may exist between breeders. Recommendations with regards to future family breeding include, for both Abagold and HIK, retaining selected breeders based on their respective contributions to the F1 progeny while reassessing the potential of remaining breeding stock under more controlled breeding conditions. No obvious trends were observed for growth with most individuals producing both faster and slower growing offspring. Juveniles will be reassessed at two years to determine whether the size advantage or disadvantages were maintained and to ascertain whether growth advantages/disadvantages may be gender specific.<br>AFRIKAANSE OPSOMMING: Die vyf mees toonaangewende perlemoen produseerders in Suid Afrika het „n genetiese verbeteringsprogram vir Haliotis midae geinisieer in „n gesamentlike poging om ekonomiese belangrike eienskappe te verbeter. Verskeie onafhanklike fokus-spesifieke studies is geinisieer, insluitend die totstandkoming van „n groeiprestasie aantekenstelsel, soos gebruik in hierdie studie, en wat noodsaaklik is om die aaneenlopende prestasie van individue te moniteer soos daar beweeg word van massa seleksie tot merker bemiddelde seleksie. Die primêre fokus van hierdie studie was die ouerskapsbepaling van F1 nageslag wat massa geselekteer is op ouderdom 1 jaar vir grootte en as of “vinniger” of “stadiger” groeiers geklassifiseer is. Nege mikrosatelliet merkers is gebruik om jong perlemoen individue en moontlike ouers te genotipeer, met die ouerskapstoekenning bereken deur CERVUS 2.0. Groei resultate vir Abagold en HIK was vergelykbaar vir beide groei groepe op drie van die lokaliteite. Geringe omgewingseffekte, alhoewel nie statisties betekenisvol nie, was sigbaar as „n groei voordeel vir jong individue op twee van die vyf lokaliteite. Ongeag maatstawe om omgewingsinvloede te standardiseer, is varieerbares moeilik om te beheer in die produksie omgewing en genotipe x omgewings interaksies mag verdere navorsing vereis en behoort in ag geneem te word in toekomstige telingsprogramme. Die onkoste wat met merker bemiddelde seleksie geassosieer word, maak die tegniek soms onaantreklik vir die meeste akwakultuur operasies; nie teen staande die genetiese voordele wat die gebruik daarvan veroorsaak. Die koste-optimiseering van roetine prosesse, soos byvoorbeeld, DNA ekstraksies, is dalk een aanslag om die addisionele koste te verminder. Chelex®100 blyk „n geskikte alternatief tot die CTAB metode te wees – die tegniek is vinnig en koste-effektief om uit te voer. Die gebruik van hierdie metode in kombinasie met die hoë deurvloei van ‟n robotiese sisteem behoort verder ondersoek te word. Vir die mikrosatelliet ontwikkeling het slegs 50% van die positiewe rekombinante klone invoegings bevat. Nukleotiedvolgorde bepaling van hierdie klone het 16% perfekte herhalings en 47% onderbroke herhalings bevat waaruit 52 inleierstelle ontwikkel en getoets is. In totaal is 31 polimorfiese mikrosatelliet loki van verskillende motiewe en samestelling ontwikkel. Een-en-sestig persent van die volgorde bepaalde klone is oortollig geag en vooraf sifting vir beide uniekheid en die teenwoordigheid van mikrosatelliete sal onnodige volgorde bepaling verhoed, die effektiwiteit van die FIASCO tegniek verhoog sowel as addisionele koste verminder. Nege loki is geselekteer vir ouerskapsbepaling. Nul allele was teenwoordig vir al die geselekteerde merkers, maar die frekwensies was egter laer as die 5% kritieke waarde. Ouerskap is 91% en 90% suksesvol bepaal vir Abagold en HIK onderskeidelik. Waarnemings dui egter daarop dat daar verwantskappe mag wees tussen van die broeidiere. Voorstelle in terme van toekomstige familie teling sluit is, vir beide Abagold en HIK, om geselekteerde broei diere te behou gebaseer op hulle onderskeie bydraes tot die F1 nageslag asook die herevaluaring van die potensiaal van die oorblywende broei diere onder meer beheerde teling toestande. Geen voor-die-handliggende tendense is waargeneem vir groei nie met die meeste individue wat beide vinniger en stadiger groeiende nageslag geproduseer het. Jong individue moet geherevalueer word op tweejarige ouderdom om te bepaal of die groei voordeel of nadele behou is en om te bepaal om groei voordele/nadele geslagspesifiek is.

Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Bacterial and fungal pathogens have developed numerous defence mechanisms against antimicrobial chemical agents, and resistance to old and new produced drugs are on the rise. Discovery of natural products derived from plants with diverse chemical structures and novel mechanisms of action to treat these notorious pathogens is a priority. Biotechnology (discussed in Chapter 1) has much to offer as a pharmacological tool and in the general study of medicinal plants. The Genus Salvia (Lamiaceae) has gathered much interest as these plants manufacture a diverse range of secondary metabolites including flavonoids, tannins and terpenoids. Of particular interest are the terpenoids which are largely implicated in the efficacy of Salvia plants as traditional medicines contributing to their pharmacological actions (discussed in Chapter 2). Due to the importance of these plants as herbal remedies, in this study, biotechnological techniques such as tissue culture and Agrobacterium-mediated transformation were applied on Salvia runcinata L.f., a South African medicinal plant, in an attempt to enhance the metabolomic profile and its bioactivity. Like so many other sages, S. runcinata has been used in folk medicine to treat a variety of ailments. Application of biotechnology was viewed as an important value adding platform for this species, assisting with its commercialisation for the cosmeceutical and pharmaceutical industries. Therefore the study had three foci: (1) to determine the seed germination behaviour and optimal conditions for micropropagation; (2) to develop a protocol that would be efficient whilst being simple for genetic transformation; and lastly, (3) to conduct phytochemical studies on in vitro generated S. runcinata transgenic hairy root and in vitro organ cultures by comparing these to glasshouse plants as potential therapeutic sources of natural compounds used in the treatment of infections in plants and humans. Data generated is thus summarised in three research chapters and Chapter 3 describes the formulated procedures assisting with in vitro seed germination and micropropagation of S. runcinata. The efficacy of smoke and scarification treatments for germination improvement was initially tested coupled to the evaluation of different hormonal combinations and different explant types which would aid with inducing adventitious shoot formation in vitro. The most effective germination treatment proved to be a 3 min exposure of seeds to 25% (w/v) H2SO4 combined with a concentration of 10-5 M smoke solution, resulting to more than 80% germination. Shoot proliferation was significantly higher using nodal explants with the addition of 4.43 μM BA. The protocol established in this part of the study is viable for large scale commercial production of S. runcinata as it would yield 1296 to 46656 viable plants in 4 to 6 months from one nodal explant. Micropropagation was applied also as a pre-emptive measure to ease pressure on the wild plants as the demand for S. runcinata is anticipated to increase due to its growing economic value as it is one of two South African sages with epi-α-bisabolol that is sought after by the pharmaceutical and cosmeceutical industries. This makes the protocol developed in this part of the study suitable for ex situ conservation of S. runcinata plantlets. Evaluations on the transgene transfer capacities of two different agropine strains (A4T and LBA 9402) of Agrobacterium rhizogenes to induce hairy root cultures of S. runcinata explants on nodal and leaf explants were conducted (reported in Chapter 4). Hairy roots formed 3 to 4 weeks after inoculation of the explants and these agropine strains showed different abilities for genetic transformation with the LBA 9402 strain producing significantly more roots on each explant compared to the A4T strain (P=0.0075). However, none of the LBA 9402 derived clones and only 2 clones generated through A4T transformation survived subculturing. The polymerase chain reaction (PCR) and reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed the presence and transcription (respectively) of rol A, rol B, rol C and ags genes which are mobilised from the transfer-DNA (T-DNA) fragment of the root-inducing (Ri) plasmid of A. rhizogenes to the plant genome during transformation. The two A4T clones, termed here A4T3 and A4T5, were stably transformed, Southern blot analysis using rol A as a probe further validated the integration of one copy of the rol A gene. Transformed hairy roots, untransformed roots from tissue cultured plants, tissue culture-derived plants and glasshouse-grown plants were profiled for secondary metabolites by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS) in Chapter 5. In this part of the study, it is clear that the use of tissue culture as a propagation system did not negatively affect the volatile compound profile of S. runcinata and plants had a similar essential oil content to that reported by Kamatou et al. (2008), leading to a conclusion that in vitro plants maintained their biochemical integrity even under an alternative micro-controlled environment. Similarly to others, Ri-transformation was explored as an avenue to alter secondary metabolism creating inter-clonal variation. Transformed clones were distinguishable, displaying more of some primary metabolites including sucrose, galactose, sorbose and fructose than the leaf extracts. With the current GC-MS methods used, this clear distinction was not obvious at the secondary metabolite level. In general, solvent extracts (acetone and methanol:dichloromethane (MetOH: DCM) (1:1 v/v) exhibited good to moderate antibacterial activity with the minimum inhibitory concentration (MIC) values ranging from 0.39 to 0.78 mg ml-1. However, in vitro plant cultures were the most potent against two Gram-negative bacterial strains: Escherichia coli (ATCC 11775) and Klebsiella pneumoniae (ATCC 13883), and two Gram-positive bacterial strains: Bacillus subtilis (ATCC 6051) and Staphylococcus aureus (ATCC 12600). The hairy root extracts did not show any activity against fungi, Fusarium subglutinans (MRC 0115) and Fusarium proliferatum (MRC 6908). Micropropagation therefore proves to be an interesting avenue for commercial production of S. runcinata, supplying plants with an improved pharmacological activity. Hence the biotechnological approach applied here is a viable strategy for the production of medicinal bioactives from S. runcinata.<br>AFRIKAANSE OPSOMMING: Bakterieë en fungi patogene het baie verskeie meganismes ontwikkel teen antimikrobiese chemiese agente, en weerstand teen ou en nuwe chemise stowwe is besig om te vergroot. Daarom is dit belangrik om natuurlike plantaardige produkte met diverse chemiese strukture en unieke werkings meganismes te ontdek waarmee hierdie berugte patogene beveg kan word. Biotegnologie (wat in Hoofstuk 1 bespreek word) kan gebruik word as 'n farmakologiese hulpmiddel in die algemene studie van plante. Die Klas (Genus) Salvia (Lamiaceae) het al baie aandag getrek aangesien hierdie plante 'n wye reeks sekondêre metaboliete vervaardig wat flavonoïede, tanniene en terpenoïede insluit. Veral van belang is die terpenoïde wat betrokke is by die doeltreffendheid van die Salvia plante as tradisionele medisyne, aangesien dit bydra tot hulle farmalogiese aksie (wat in Hoofstuk 2 bespreek word). Aangesien hierdie plante sulke belangrike kruie is, word daar in hierdie studie, biotegnologiese tegnieke soos die kweek van weefsel en Agrobacterium-bemiddelde transformasie op Salvia runcinata L.f. toegepas om die metabologiese profiel en die bioaktiwiteit daarvan te verbeter. Soos baie van die salies is S. runcinata tradisioneel dikwels gebruik om allerhande siektetoestande te behandel. Die toepassing van biotegnologie word beskou as 'n belangrike manier om waarde by te voeg sodat hierdie plant kommersieei deur die kosmetiese en farmakeutiese bedrywe gebruik kan word. Daarom is daar op drie dinge gefokus: (1) die ontkiemings gedrag van saad en die optimale toestande vir mikrovoortplanting (2) die ontwikkeling van protokol wat eenvoudig maar doeltreffend is vir genetiese transformasie, en die (3) fito-chemise studies op in vitro genereerde S. runcinata transgeniese harige wortels en in vitro orgaan kwekings deur om hulle te vergelyk met kweekhuis plante as potentiële terapeutiese bronne van natuurlike samestellings vir die behandeling van infeksies in beide plante en mense. Die data wat gegenereer is, is opgesom in drie hoofstukke, en in Hoofstuk 3 word die prosedures wat gebruik word in die in vitro saad ontkieming en die mikro voortplanting van S. runcinata, bespreek. Die doeltreffendheid van rook en skarifikasie behandeling vir die verbetering van ontkieming is eers getoets en gekoppel aan die evaluering van verskillende hormoonkombinasies en verskillende eksplant tipes wat lei tot die formasie van uitloopsels in vitro. Daar is gevind dat die effektiefste behandeling vir ontkieming, 'n 3-minuut blootstelling van saad aan 25% (w/v) H2SO4 gekombineer met 'n konsentrasie 10-5 M rook oplossing is. Dit het gelei tot meer as 80% ontkieming. Daar was baie meer uitloopsels toe nodale eksplante gebruik is met die byvoeging van 4.43 μM BA. Die proktokol wat hier gevestig is, kan op groot skaal gebruik word vir die kommersiële produksie van S. runcinata, want 1296 tot 46656 lewensvatbare plante kan binne 4 ot 6 maande van een nodale eksplant gemaak word. Mikro voortplanting is toegepas as 'n voorkomende maatreel om die druk op die natuur te verminder omdat daar verwag word dat die vraag na S. runcinata sal toeneem na gelang die groeiende ekonomiese waarde daarvan toeneem. Dit is een van twee Suid-Afrikaanse salies met epi-α-bisabolol wat deur die farmakeutiese en die kosmetiese bedrywe gebruik word. Dit beteken dat die protokol wat hier ontwikkel is, geskik is vir die ex situ bewaring van S. runcinata plante. Die transgeen oordrag van twee verskillende agropien tipes (A4T and LBA 9402) van Agrobacterium rhizogenes is geevalueer (en in Hoofstuk 4 beskryf). Harige wortels het 3 tot 4 weke na die inenting van die eksplante gevorm en hierdie agropien tipes het verskillende vermoëns vir genetiese transformasie getoon, met die LBA 9402 tipe wat baie meer wortels op elke eksplant voorgebring het in vergelyking met die A4T tipe (P=0.03116). Geen van die LBA 9402-afgeleide klone en slegs 2 klone wat deur A4T transformasie genereer is, het oorleef. The polimerase ketting reaksie (PCR) en die teenoorgestelde trenskriptasie-polimerase (RT-PCR) ketting reaksie het die teenwoordigheid en transkipsie (onderskeidelik) van rol A, rol B en rol C en ags gene, wat oorgedra word deur die oordrag DNA (T-DNA) fragment van die wortel induserende (Ri) plasmied van A. rhizogenes na die plant genoom tydens transformasie, bevorder. A4T klone, hier A4T3 and A4T5 genoem, is stabiel transformeer. Southern blot ontleding het met die gebruik van rol A, die integrasie van een kopie van die rol A geen, bevestig. In Hoofstuk 5 is transformeerde harige wortels, ongetransformeerde wortels van weefsel gekweekte plante, weefsel gekweekte plante, en kweekhuis plante deur dun-laag chromatografie (TLC) en gas-chromatografie-massa spektrometrie (GC-MS) geprofiel vir sekondêre metaboliete. In hierdie deel van die studie is dit duidelik dat die gebruik van weefsel kwekery as 'n voortplantsisteem nie 'n negatiewe effek gehad het op die vlugtige samestelling profiel van S. runcinata nie en dat plante 'n sootgelyke essentiële olie inhoud het as wat deur Kamatou et al. (2008) bevind is. Dit lei tot die gevolgtrekking dat in vitro plante hulle biochemiese integriteit behou selfs onder alternatiewe mikro-beheerde omgewings. Ri-transformasie is ondersoek as 'n manier om sekondêre metabolisme te verander om interkloon variasie te skep. Getransformeerde klone kon uitgeken word, aangesien dit meer primêre metaboliete soos sukrose, galaktose en fruktose insluit as die blaar ekstrakte. Hierdie verskil was nie met die huidige GC-MS metodes so duidelik sigbaar op die sekondêre metabolitiese vlak nie. Oor die algemeen toon ekstraksie met asetoon en methanol dichlorometaan (MetOH: DCM) (1:1 v/v) goeie tot gemiddelde antibakteriese aktiwiteit met die minimum remmende konsentrasie (MIC) waardes van 0.39 tot 0.78 mg ml-1. Die in vitro plant kulture het egter sterker weerstand gebied teen twee Gram-negatiewe bakteriese tipes: Escherichia coli (ATCC 11775) en Klebsiella pneumoniae (ATCC 13883), en teen twee Gram-positiewe bakteriese tipes: Bacillus subtilis (ATCC 6051) en Staphylococcus aureus (ATCC 12600). Die harige wortel ekstrakte het geen aktiwiteit teen die swamme, Fusarium subglutinans (MRC 0115) en Fusarium proliferatum (MRC 6908) getoon nie. Mikro-voortplanting is dus 'n interessante manier om S. runcinata kommersieel te produseer aangeien die plante verbeterde farmalogiese aktiwiteit toon. Die biotegnologiese benadering wat hier toegepas word, is 'n praktiese strategie vir die produksie van geneesmiddels van S. runcinata.

Thesis (DLitt)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: This dissertation looks at the reception of Gen. 1-3, one of the most controversial parts in the Hebrew Bible. How was it interpreted by the Nguni speaking communities (e.g. Xhosa, Zulu, siSwazi and siNdebele) taking into consideration their background, culture and religious belief system? The reception approach is followed in the research because of its emphasis on the role of the reader in understanding texts. Sources that are utilized are Nguni Bible translations, selected preached sermons (which the researcher attended himself), Nguni stories and folk tales and reviews undertaken among selected Nguni groups. A close-reading of the texts under discussion is undertaken in order to determine the basic content and issues of interpretation involved. The central concepts of cosmogony as contained in Gen. 1-2 are studied, as well as the story of the Garden of Eden and the concept of the “fall” in Gen. 3. The map of the Nguni language group is described and the culture and belief system of the Nguni speaking communities. Central concepts to this belief system are the worship of ancestors, marriage, circumcision, and among the Swazis the incwala (annual national feast) Legends and folk tales were used as sources for the Nguni belief system. It was determined that the Nguni speaking people worshipped one God in their traditional way, but always through their ancestors as a sign of respect. The role of the missionaries is analyzed by describing the history of the various missionary societies and their influence on the Nguni peoples. A very short discussion is devoted to preached sermons by Nguni pastors in the Seventh-day Adventist Church.Bible translations have always played a very important role among Nguni speakers (both Christians and non-believers). The need for translations using understandable contemporary terms is emphasized. This is the challenge to the Bible societies and Bible translators. Qualitative reviews were undertaken under selected Nguni speaking groups (Xhosa, Zulu, siSwati and siNdebele). Some of the results obtained from these reviews (full transcripts are included) are: (1) that there is a common understanding of the origin of the universe between the Hebrew Bible and the Nguni religious culture. (2) Serpent (Gen. 3): among the Zulus this concept is understood in terms of sexuality, but it can also be linked with the ancestors. (3) Both communities (Hebrew Bible and the Nguni) were tainted with the concept and ideology of patriarchalism. The crucial question in the research was: “what happens when a cosmogonic myth is transferred from one community to another?” In the case of Gen. 1-3 an ancient Hebrew text was transmitted to African cultures via missionaries and Bible translations. Nguni people react differently. Whereas some accept Gen. 1-3 (cosmogonies and the “fall”) as a detailed explanation of how creation and the “fall” came about, others reject it.<br>AFRIKAANSE OPSOMMING: Hierdie proefskrif kyk na die resepsie van Gen. 1-3, een van die mees kontroversiële dele in die Hebreeuse Bybel. Hoe word dit geïnterpreteer deur die Ngunisprekende gemeenskappe (Xhosas, Zoeloes, Swazi’s en Ndebele-groepe), met inagneming van hulle agtergrond, kultuur en stelsel van godsdienstige oortuigings? Die resepsiebenadering word in hierdie navorsing gevolg weens die klem op die rol van die leser in hoe tekste verstaan word. Die bronne wat aangewend is, is Ngunibybelvertalings, geselekteerde preke (wat die navorser self bygewoon het), Ngunistories en -volksverhale, en onderhoude wat met geselekteerde Ngunigroepe gevoer is. 'n In-dieptestudie van die betrokke tekste is onderneem ten einde die basiese inhoud en interpretasiekwessies te bepaal. Die sentrale konsep van kosmogonie, soos vervat in Gen. 1- 2, is bestudeer, asook die storie oor die Tuin van Eden en die konsep van die “sondeval” in Gen. 3. Die kaart van die Ngunitaalgroep word beskryf, asook die kultuur en geloofstelsel van die Ngunisprekende gemeenskappe. Sleutelkonsepte in hierdie geloofstelsel is die aanbidding van voorvaders, die huwelik, besnydenis, en onder die Swazi’s, die incwala (jaarlikse nasionale fees). Legendes en volksverhale is gebruik as bronne vir die Ngunigeloofstelsel. Daar is vasgestel dat die Ngunisprekende mense altyd een God aanbid het op hulle tradisionele manier, maar altyd deur voorvaders as 'n teken van respek. Die rol van die sendelinge word ontleed deur die geskiedenis van die verskeie sendinggenootskappe te beskryf, asook hulle invloed op die Ngunimense. 'n Baie kort bespreking word gewy aan preke gelewer deur Ngunipastore in die Sewendedaagse Adventistekerk.Bybelvertalings het nog altyd 'n baie belangrike rol gespeel onder Ngunisprekers (beide Christene en nie-gelowiges). Die behoefte vir vertalings wat verstaanbare, kontemporêre terme gebruik, word beklemtoon. Dít is die uitdaging wat aan die bybelgenootskappe en bybelvertalers gestel word. Kwalitatiewe onderhoude is afgelê onder geselekteerde Ngunisprekende groepe (Xhosas, Zoeloes, Swazi’s en Ndebele-groepe). Resultate wat verkry is van hierdie evaluerings (waarvan volledige transkripsies voorsien word) sluit in: (1) dat daar 'n gemeenskaplike begrip is van die oorsprong van die heelal by die Hebreeuse Bybel en die Ngunigeloofskultuur; (2) dat die konsep van die slang (Gen. 3) onder die Zoeloes in terme van seksualiteit verstaan word, maar dat dit ook met die voorvaders in verband gebring kan word; en (3) dat beide gemeenskappe (Hebreeuse Bybel en die Nguni) tekens dra van die konsep en ideologie van patriargisme. Die kernvraag in die navorsing was: “Wat gebeur wanneer ’n kosmogoniese mite oorgedra word van een gemeenskap na 'n ander?”. In die geval van Gen. 1-3 is 'n ou Hebreeuse teks oorgedra na Afrikakulture via sendelinge en bybelvertalings. Ngunimense reageer verskillend. Waar sommige Gen. 1-3 aanvaar (kosmogonieë en die “sondeval”) as 'n gedetailleerde verduideliking van hoe die skepping en die “sondeval” plaasgevind het, word dit deur ander verwerp.

This thesis addresses how demographic processes affect both genetic and cultural variation. Drawing on theory and techniques from both gene-culture coevolution and population genetics, and using both genetic and archaeological data, I present a number of projects covering a wide range of questions on the evolutionary history of our species. Chapter 2 develops a simulation model of the gene-culture coevolution of lactase persistence and dairying in Neolithic Europe. Using approximate Bayesian computation (ABC) to integrate modern genetic and archaeological data, the results demonstrate that this coevolutionary process began —7,500 years ago in central Europe. The inferred origin is closely associated with the emergence of the Linearbandkeramik (LBK), an early cattle-based dairying culture. Chapter 3 extends a previous cultural evolutionary model to show that heterogeneity in subpopulation density causes spatial structuring of culturally inherited skill accumulation. Genetic estimates of regional effective population density demonstrate that the appearance of modern human behaviour during the Late Pleistocene can be explained by demographic factors. Chapter 4 reviews the application of the neutral model in archaeological and other cultural contexts, and develops a novel statistical approach to test for deviation from neutrality. Results show that surprisingly high levels of non-neutrality, in the form of frequency- dependent copying, can go undetected. Chapter 5 develops a novel neutral model of cultural evolution, relaxing some previous common assumptions outlined in Chapter 4. The model allows accurate estimation of population parameters from minimal archaeological data, and is applied to a decorated pottery dataset from the LBK settlements of southwest Germany. Chapter 6 outlines the utility of the coalescent model of population genetics in inferring potentially complex demographic histories using both modern and ancient DNA samples. I provide two examples of my work from collaborative projects on the domestication of Near Eastern cattle and the demographic history of the Hispaniolan hutia.

Thesis (PhD (Genetics))--University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: South African abalone, Haliotis midae, has been the subject of major concern regarding its survival and conservation over the last decade or more. Being the only one of five endemic species with commercial value, there is considerable interest and urgency in genetic management and improvement of this species. Limited genetic information and the increasing conservation concern of this species are considered the key motivations for generating information on the micro- and macro-evolutionary processes of H. midae, the overall objective of this study. This study reported the first microsatellite and Single Nucleotide Polymorphism (SNP) markers developed specifically for Haliotis midae. Both these marker types were applied to elucidate the degree of gene flow in nine natural abalone populations whilst testing for two contrasting hypotheses; panmixia versus restricted gene flow. Data was analysed using a series of methodological approaches ranging from traditional summary statistics to more advanced MCMC based Bayesian clustering methods with and without including spatial information. Using only microsatellite data, the historical demography of the species was also examined in terms of effective population size and population size fluctuations. Finally, the evolutionary positioning and origin of Haliotis midae with regards to other Haliotis species was investigated based on mitochondrial and nuclear sequence data. Both microsatellite and SNP data gave evidence for subtle differentiation between West and East coast populations that correlates with a hydrogeographic barrier in the vicinity of Cape Agulhas. Population substructure was supported by AMOVA, FCA and Bayesian clustering analysis. Clustering utilizing spatial information further indicated clinal variation on both sides of the proposed barrier with a region in the middle coinciding with a secondary contact zone, indicating possible historical isolation during glacial periods. Overall, the similar degree of substructure observed with both microsatellites and SNPs supported the existence of contemporary and/or historical factors with genome-wide effect on gene flow. The population expansion measured with the microsatellites was inconsistent with the known recent decline but taking the species’ life cycle and large effective population size into account, a shrinkage in population size will probably only be apparent in a few generations time. On a macro-evolutionary scale, this study presents the first classification of South African abalone as a monophyletic group within the Haliotidae family. The topology based on the combined mitochondrial and nuclear dataset is highly suggestive of a relatively recent radiation of the SA species from the Indo-Pacific basin. The study concludes by describing the most likely factors that could have affected overall population structure and makes suggestions on how the given genetic information should be incorporated into strategies aimed towards the effective management and conservation of Haliotis midae.<br>AFRIKAANSE OPSOMMING: Die Suid-Afrikaanse perlemoen, Haliotis midae, is oor die laaste dekade of meer die onderwerp van groot bekommernis betreffende die spesie se oorlewing en bewaring. Aangesien dit die enigste van vyf endemiese SA spesies is met kommersiёle waarde, is daar besonderse belang en erns in die genetiese beheer en verbetering van die spesie. Beperkte genetiese inligting en ‘n toenemende behoefte om die spesie te bewaar is die hoof motivering agter die generering van informasie rakende mikro- en makro-evolusionêre prosesse in Haliotis midae en is die oorhoofse doel van hierdie studie. Hierdie studie beskryf die eerste mikrosatelliete en enkel basispaar polimorfismes wat ontwikkel is spesifiek vir Haliotis midae. Beide tipe merkers is aangewend om die mate van gene vloei in nege wilde perlemoen populasies te ondersoek terwyl twee hipoteses ondersoek is; panmiksie versus beperkte gene vloei. Data is geanaliseer deur gebruik te maak van ‘n reeks metodieke benaderings wat wissel van tradisionele opsommings statistieke tot meer gevorderde MCMC gebasseerde groeperings metodes met of sonder die gebruik van geografiese data. Mikrosatelliet data is ook aangewend om die historiese demografie van die spesie te bepaal in terme van effektiewe populasie grootte asook veranderinge in populasie groottes. Laastens is die evolusionêre posisionering en oorsprong van Haliotis midae teenoor ander Haliotis spesies ondersoek deur gebruik te maak van mitokondriale en nukleêre DNA volgorde data. Beide mikrosatelliet en enkel basispaar polimorfisme data lewer bewys van ‘n subtiele genetiese verskil tussen wes en ooskus populasies wat verband hou met ‘n hidrografiese skeiding in die omgewing van Kaap Agulhas. Populasie struktuur is ondersteun deur die analise van molekulêre variansie (AMOVA), faktoriale komponente analise asook Bayesiese groeperings analise. Groeperings analise wat geografiese informasie insluit dui klinale genetiese variasie aan beide kante van die skeiding aan met ‘n area in die middel wat ooreenstem met ‘n sekondêre kontak gebied. In totaal, ondersteun die soortgelyke mate van struktuur verkry met beide die mikrosatelliete en enkel basispaar polimorfismes die bestaan van hedendaagse en/of historiese faktore met genoom wye invloed op gene vloei. Die toename in populasie grootte vasgestel deur die mikrosatelliet data stem nie ooreen met die onlangse afname waargeneem in die spesie nie, maar met inagneming van Haliotis midae se lewenssiklus en groot effektiewe populasie grootte, sal die afname in populasie grootte moontlik eers oor ‘n paar generasies na vore kom. Op ‘n makro-evolusionêre skaal lewer hierdie studie die eerste klassifikasie van Suid-Afrikaanse perlemoen as ‘n monofiletiese groep binne die Haliotidae familie. Die topologie gebaseer op ‘n gesamentlike mitkondriale en nukleêre datastel is hoogs aanduidend van ‘n relatiewe onlangse verspreiding van die Suid-Afrikaanse spesies uit die Stille-Indiese Oseaan. Die studie sluit af deur die mees algemene faktore te bespreek wat populasie struktuur kon beïnvloed het en maak voorstelle op watter wyse hierdie genetiese inligting aangewend kan word vir die effekiewe beheer en bewaring van Haliotis midae.

Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Apr. 25, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of English." Discipline: English; Department/School: English.

This thesis interrogates the relationship between Mexican nationalism, maize agriculture and contemporary technoscience. My aim is to unlock a phenomenon that can at times take the shape of a reactive nationalism, while positioning itself as a defense of maize agriculture. Since 1999, a growing coalition of Mexican and international activists has denounced the transgenic "contamination" of Mexican maize agriculture. In the process, activists have identified transgenic maize as the instrument of a foreign assault on a sovereign entity, namely the nation itself – which "native maize" symbolizes in a very tangible way. Rather than being positioned as a mere instrument of foreign powers, in my argument agricultural biotechnology is seen as a non-deterministic event that calls for a critical assessment of national narratives around agriculture, science, technology and technoscience. In addition to developing such a critical assessment, I set out to explore the ethical and political promises of refiguring the activist use of the term "contamination" so that the latter is understood to pertain genetically to identity itself, including the maize-based identity that some Mexicans invoke in their nationalistic opposition to transgenic maize. Drawing on specific contributions from post-Marxist political theory, media and cultural studies and feminist technoscience, I position "genetic contamination" as a critical and creative alternative to the reproduction of nationalist identifications. An acknowledgment of the ineradicability of antagonism, a rigorous attention to contextual specificities and a materialist commitment to the pursuit of democracy in the technoscientific world all inform my engagement with the nationalist narratives in the context of technoscience, understood here as "a form of life, a generative matrix" (Haraway, Modest_Witness 50).

Thesis (MSc)--University of Stellenbosch, 2007.<br>ENGLISH ABSTRACT: The indigenous abalone Haliotis midae is one of the most remarkable and highly exploited species of marine molluscs in South Africa. It is the only species of southern African Haliotidae to be commercially reared and has been successfully cultured for almost two decades. Its short history of domestication along with market demands and the need to develop efficiency in the production process has resulted in an increased interest in the possible genetic improvement of this species. The unhurried growth rate associated with H. midae is a cause of particular concern to the industry, predominantly with regards to profitability and competitiveness in the market place. A modest amount of work has so far been directed at establishing a means of enhancement for selective breeding on the commercial level. Genetics plays a key role in the establishment of successful improvement programmes in various aquaculture species. The aim of this study was to develop species-specific microsatellite markers for the abalone and subsequently perform parentage assignment on farm produced animals entered into a growth performance recording scheme. Animals were obtained from the hatcheries of three commercial abalone farms situated in the Walker Bay region in the Western Cape. Microsatellites were isolated using the enrichment-based FIASCO method, and characterised into perfect, imperfect and compound repeats according to the structural nature of their repetitive units. From the partial gDNA libraries obtained and 365 screened colonies, a total of 54 loci were located. PCR primers were designed for 36 markers and the 15 primer pairs that displayed loci with the highest level of polymorphism were subsequently chosen for fluorescent labelling. The markers were tested on a subset of 32 wild H. midae individuals to determine their usefulness and efficiency in genotyping. Five markers, along with five others that were previously designed, were chosen for assigning parentage to the animals submitted to the performance recording scheme. Three thousand offspring from each of the three participating farms were equally divided and reared at five different locations. From each location 20 fast growing and 20 slow growing juveniles, as well as the broodstocks, were sampled and genotyped using the ten chosen microsatellite loci. Two farms had 60% of offspring unambiguously assigned to a single parental couple. Assignments showed patterns of dominant male and female brooders, but no trend in brooders specifically contributing to fast or slow growing offspring. Parentage assignment for the third farm was, however, unsuccessful due to lack of broodstock data. In future, screening of all available broodstock will ensure acquisition of relevant pedigree information. The results obtained in this study are an initial step in the development of a genetic improvement programme for commercial Haliotis midae.<br>AFRIKAANSE OPSOMMING: Die inheemse skulpvis Haliotis midae is een van die mees merkwaardige en hoogs oorbenutte mariene slakspesies in Suid-Afrika. Dit is die enigste suidelike Afrika Haliotidae spesie wat kommersieel benut word en dit word al meer as twee dekades suksesvol geteel. Die spesie se kort domestiseringsgeskiedenis, toenemende mark aanvraag en die behoefte om meer effektiewe produksie daar te stel, het gelei tot toenemende belangstelling in die moontlike genetiese verbetering van die spesie. Die stadige groeitempo geassosieer met H. midae is veral ‘n punt van kommer vir die industrie, veral in terme van winsgewendheid en kompetering in die markplek. Minimale werk is sover gedoen in die daarstelling van verbetering deur selektiewe teling op ‘n kommersiële skaal. Genetika speel ’n sleutelrol in die daarstelling van suksesvolle verbeteringsprogramme van verskeie akwakultuur spesies. Die doel van hierdie studie was om spesie-spesifieke mikrosatelliet merkers vir perlemoen te ontwikkel en vervolgens ouerskapsbepaling van kommersiële diere, wat deelneem aan ‘n groeiprestasie aantekenstelsel, uit te voer. Diere is voorsien deur die teelstasies van drie kommersiële perlemoenplase geleë in die Walker Bay omgewing in die Wes-Kaap. Mikrosatelliete is geïsoleer deur die verrykings-gebaseerde FIASCO metode, en gekarakteriseer as perfekte, onderbroke of saamgestelde herhalings gebaseer op die strukturele aard van die herhalings eenhede. Vanaf die gedeeltelik gDNA biblioteke wat bekom is en 365 gesifte kolonies, is ‘n totaal van 54 loki opgespoor. PKR inleiers is ontwerp vir 36 merkers en die 15 inleierpare, wat loki met die hoogste polimorfisme geamplifiseer het, is vervolgens geselekteer vir fluoreserende merking. Die merkers is getoets op ’n kleiner groep van 32 natuurlike H. midae individue om hulle bruikbaarheid en genotiperingseffektiwiteit te bepaal. Vyf merkers is saam met vyf reeds ontwikkelde merkers gekies vir ouerskapsbepaling van die diere in die prestasie aantekenstelsel. Drieduisend nageslag diere vanaf elkeen van die drie deelnemde plase is gelykop verdeel en grootgemaak op die vyf verskillende lokaliteite. ‘n Monster van 20 vinnig groeiende en 20 stadig groeiende jong perlemoen, sowel as broeidiere, is vanaf elke lokaliteit geneem en gegenotipeer deur middel van die 10 geselekteerde mikrosatelliet loki. Sestig persent van twee van die plase se nageslag is onteenseglik toegesê aan ‘n enkele ouerpaar. Ouerskapstoekenning het patrone van dominante vroulike en manlike broeidiere getoon, maar geen tendens in terme van bydrae tot vinnig en stadig groeiende nageslag kon gevind word nie. Ouerskapstoekenning vir die derde plaas was onsuksesvol as gevolg van ’n gebrek aan data vir die broeidiere. In die toekoms sal genotipering van alle beskikbare broeidiere die daarstelling van relevante stamboominligting verseker. Die resultate verkry in hierdie studie verteenwoordig ‘n eerste stap in die ontwikkeling van ’n genetiese verbeteringsprogram vir kommersiële Haliotis midae.

Thesis (MSc)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Haliotis midae (locally also known as perlemoen) is the largest of five endemic species found along the coast of South Africa. It is the only species with commercial value contributing to the exploitation of these animals. Due to declines of natural stocks, farming practices were established during the early 1990s in order to supply the international demand. To facilitate efficient breeding methods and ensure the sustainability of these commercial populations, genetic management, which can be accomplished with the use of molecular markers such as single nucleotide polymorphisms (SNPs), is necessary. Single nucleotide polymorphisms have become the markers of choice in various applications in aquaculture genetics due to their abundance in genomes, reduction in developmental costs and increased throughput of genotyping assays. Identification of SNPs in non-model species such as H. midae can be achieved by in silico approaches. In silico methods are suitable for de novo SNP identification and are both cost- and time-efficient. It is based on the analysis of multiple alignments where mismatches may be reported as candidate SNPs. Various medium-throughput genotyping methods are available to confirm putative SNPs, but the ideal method depends on factors such as cost, accuracy and multiplexing capacity. Although SNP markers can have various applications within the aquaculture environment the focus for this current study was saturating the linkage map of H. midae with additional markers. This would assist in the identification of quantitative trait loci associated with economically important traits, which in turn could ultimately be employed for marker-assisted selection and improved molecular breeding programs. In order to identify in silico SNPs, sequenced transcriptome data from a previous study was used and subjected to a series of criteria: minor allele frequency 10%, minimum coverage 80, 60 bp flanking regions. Selected loci were genotyped using a 192-plex assay with the Illumina GoldenGate genotyping assay with the VeraCode technology on the BeadXpress platform, in individuals from six mapping families. A conversion rate of 69.35% and global success rate of 76.34% was achieved. Polymorphic loci were subjected to linkage analysis using JoinMap® v.4.1 to create sex-average and sex-specific maps and to saturate the current linkage map for H. midae. Along with previously developed markers, 54% of the newly developed SNPs could be successfully incorporated into the linkage map of H. midae. A total of 18 linkage groups were observed with an average marker spacing of 6.9 cM and genome coverage of 79.1%. Bioinformatic analyses and setting stringent criteria to identify SNPs from sequenced transcriptomic data proved to be an efficient way for SNP discovery in the current study. Genotyping of the identified loci with the GoldenGate genotyping assay demonstrated a high success rate; providing a genotyping assay adequate for species with little genomic information. The linkage map created in this study illustrated the utility of SNP markers in conjunction with microsatellite markers for linkage map construction and the adequate marker spacing obtained provides a step closer to quantitative trait loci mapping in this species.<br>AFRIKAANSE OPSOMMING: Haliotis midae (plaaslik ook bekend as perlemoen) is die grootste van vyf inheemse spesies wat langs die kus van Suid-Afrika aangetref word. Dit is die enigste spesie van kommersiële waarde wat bydraend is tot die uitbuiting van hierdie diere. As gevolg van die afname in hierdie natuurlike hulpbron het boerdery praktyke gedurende die vroeë 1990's ontstaan om in die internasionale aanvraag te voorsien. Ten einde doeltreffende teelmetodes te beoefen en die volhoubaarheid van hierdie kommersiële populasies te verseker is genetiese bestuur, wat bewerkstellig kan word deur die gebruik van molekulêre merkers soos enkel nukleotied polimorfismes (ENPs), baie belangrik. Enkel nukleotied polimorfismes is gewilde merkers in verskeie toepassings in akwakultuur genetika as gevolg van hul oorvloed in genome, verlaagde ontwikkelingskoste en verhoogde deurset van ENP-genotiperingstoetse. Identifisering van ENPs in nie-model spesies soos H. midae kan uitgevoer word deur in siliko benaderings te gebruik wat geskik is vir de novo ENP identifisering en ook tyd- en koste-effektief is. Dit word gebaseer op die analise van veelvuldige inlynstellings waar nukleotiedes wat nie ooreenstem nie as kandidaat ENPs gerapporteer kan word. Om kandidaat ENPs te bevestig, kan verskeie medium-deurset genotiperingsmetodes uitgevoer word, maar die ideale metode word bepaal deur faktore soos koste, akkuraatheid en multipleks kapasiteit. Alhoewel ENP merkers in verskeie toepassing binne die akwakultuur omgewing gebruik kan word was die fokus van die huidige studie om die koppelingskaart van H. midae te versadig. Dit sal bydrae tot die identifisering van kwantitatiewe eienskap lokusse wat gekoppel kan word aan ekonomies belangrike eienskappe wat dan op die beurt weer vir merkerbemiddelde seleksie gebruik kan word en uiteindelik ten opsigte van die verbetering van molekulêre teelprogramme aangewend kan word. Ten einde in siliko ENPs te identifiseer is transkriptoomdata van 'n vorige studie gebruik en onderwerp aan 'n reeks kriteria: geringste alleelfrekwensie 10%, minimum dekking 80, 60 bp gebiede weerskante van polimorfisme. Geïdentifiseerde lokus-genotipering is met behulp van 'n 192-pleks toets uitgevoer met die Illumina GoldenGate genotiperingstoets met die VeraCode tegnologie op die BeadXpress-platform, in individue afkomsitg vanaf ses karteringsfamilies. 'n Omskakelingskoers van 69.35% en 'n algehele sukseskoers van 76.34% is bereik. Polimorfiese lokusse is onderwerp aan koppelings-analise met behulp van JoinMap® v.4.1 om geslags-gemiddelde en geslags-spesifieke kaarte te skep asook om die kaart wat beskikbaar is vir H. midae te versadig. Saam met voorheen ontwikkelde merkers is 54% van die nuut ontwikkelde ENPs suksesvol opgeneem in die kaart van H. midae. 'n Totaal van 18 koppelingsgroepe is verkry met 'n gemiddelde merker-spasiëring van 6.9 cM en 'n genoomdekking van 79.1%. Die gebruik van bioinformatiese analises en streng kriteria om ENPs vanaf transkriptoomdata te identifiseer blyk doeltreffend te wees in hierdie studie. Genotipering van die geïdentifiseerde lokusse met die GoldenGate genotiperingstoets dui op 'n hoë suksessyfer en verskaf 'n voldoende genotiperingstoets aan spesies met min genomiese inligting. Die koppelingskaart in hierdie studie het geïllustreer dat die ENP merkers suksesvol saam met mikrosatelliet merkers gebruik kan word vir koppelingskaart konstruksie en dat die voldoende merker-spasiëring verkry 'n stap nader aan kwantitatiewe eienskap lokus kartering in hierdie spesie bied.

This thesis analyses the emergence and evolution of the think-tanks in Turkey. It seeks primarily to answer to whether or not it is possible to mention &lsquo<br>a think-tank culture&rsquo<br>in Turkey. After the definition, characteristics and types of the think-tank term are debated, the historical background of think-tanks all over the world is analyzed. The thesis looks at similarities and differences among the think-tanks in and outside Turkey in terms of their size and areas of specialization. It also tries to find out who are pioneering to the establishment of think-tanks, whom the think-tanks are serving, which financial resources and functions they have in Turkey.

This thesis investigates development of generic capabilities in an RMIT undergraduate logistics degree program. Generic capabilities are those general graduate attributes that are not specifically discipline-focused, examples being communication and teamwork skills. A major research objective of this thesis is the extent to which graduates perceived that generic capabilities were developed in their RMIT logistics undergraduate program, specifically in a cross-cultural context spanning a range of organisations differing in size and ownership structure. The thesis involves two studies. In Study 1 managers from eight organisations, ranging in size from multi-national to small public and private (family-owned) companies were interviewed to develop a series of qualitative organisational case studies using grounded theory methodology. Study 2 is a quantitative survey of 31 Australian and 25 Asian (Singapore and Hong Kong) logistic graduates from 1996 to 2002. In Study 1, generic capabilities rankings in different organisations varied, depending on whether managers being interviewed were operational or human resource management specialists, but there was general agreement that communication, problem-solving, initiative and enterprise, and teamwork skills were highest priority. Study 2 results indicate that the views of both Asian and Australian graduates are in line with the management rankings, and are consistent with those reported by Australian and OECD government and industry research organisations. Both graduate groups agree that generic capabilities are covered in the RMIT logistic program, but ratings are generally in an 'adequate' to 'good' range, with no outstanding features. Although cultural diversity in the student body is seen as a major benefit, there are little data indicating a high level of Australian and Asian student networking, and a number of respondents are critical of a lack of international focus in the present program. A major issue is a n eed for more emphasis on presentation and problem-solving skills so graduates are able to carry through a project from initiation to completion.

This thesis explores the development of Virginia Woolf’s late cultural criticism. While contemporary scholars commonly observe that Woolf shifted her intellectual focus from modernist fiction to cultural criticism in the 1930s, there has been little sustained examination of why and how Woolf’s late cultural criticism evolved during 1930-1941. This thesis aims to contribute just such an investigation to field. My approach here fuses a feminist-historicist approach with the methodology of genetic criticism (critique génétique), a French school of textual studies that traces the evolution of literary works through their compositional histories. Reading across published and unpublished texts in Woolf’s oeuvre, my genetic, feminist-historicist analysis of Woolf emphasises that her late cultural criticism developed from her early feminist politics and dissident aesthetic stance as well as in response to the tempestuous historical circumstances of 1930-1941. As a prelude to my investigation of Woolf’s late output, Chapter 1 traces the genesis of Woolf’s cultural criticism in her early biographical writings. Chapter 2 then scrutinises Woolf’s late turn to cultural criticism through six essays she produced for Good Housekeeping in 1931. Chapter 3 surveys the evolution of Woolf’s critique of patriarchy in Three Guineas (1938) through the voluminous pre-publication documents that link this innovative feminist-pacifist pamphlet to The Years (1937). Finally, Chapter 4 outlines how Woolf’s last novel, Between the Acts (1941), fuses fiction with cultural criticism to debate art’s social role in times of national crisis. The close relationship between formal and political radicalism in Woolf’s late cultural criticism, I conclude, undermines the integrity of viewing Woolf’s oeuvre in two distinct phases –the modernist 1920s and the socially-engaged 1930s – and suggests the danger of using such labels in wider narratives of interwar literature. Woolf’s late cultural criticism, this thesis argues, developed from rather than rejected her earlier experimentalism.

Made available in DSpace on 2014-06-11T19:26:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-06-22Bitstream added on 2014-06-13T19:33:23Z : No. of bitstreams: 1 santos_rb_me_jabo.pdf: 1467164 bytes, checksum: 5cce9136001027d260fc80ff1c5229f4 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Os objetivos do presente trabalho foram (i) identificar uma cultivar de soja (Glycine max) com melhor resposta in vitro para indução de calos e regeneração; (ii) otimizar o processo de transformação genética via biobalística em embriões oriundos de cotilédone maduros de soja. Os experimentos de indução de calos e transformação genética foram realizados com as cultivares IAS-5, BR16 e MSOY6101. Para indução de calos foram utilizadas altas doses de auxinas [5 mg/L 2,4D (2,4-diclorofenoxiacético- 2,4D), 5 mg/L 2ip (2-isopenteniladenina) e 10 mg/L ANA (ácido-naftalenacético)] e para regeneração de 0 a 2 mg/L de BAP (6-benzylaminopurine), adicionando-se 0,5 mg/L de ANA em todos os tratamentos. Vários parâmetros foram testados na transformação: concentração do tungstênio; BAP (0 a 5 mg/L) no co-cultivo e a concentração de canamicina para seleção de plantas. A construção utilizada foi constituída pelo vetor pCAMBIA 2301, contendo o gene repórter GUS, regulado pelo promotor constitutivo 35SCaMV e adição do gene de seleção NPTII que confere resistência a canamicina. Foram observadas diferenças para a indução e regeneração via embriogênese somática entre as cultivares de soja, resultando na seleção da cultivar 'IAS-5' para avaliação do potencial embriogênico quanto à diferenciação e regeneração de plantas. Essa cultivar apresentou alta taxa de indução, proliferação e regeneração estável. Os resultados mostraram que a adição de 0,5 mg/L de BAP ao meio de regeneração e 5 mg/L de 2,4D ao meio de indução aumentaram as taxas de diferenciação dos embriões de 0 para 27,77%. A partir dos resultados observados na transformação genética em N. benthamiana, adotou-se 24 mg/μL de tungstênio por membrana para as três cultivares de soja. Para regeneração em embrião maduro de soja o melhor resultado...<br>The present work aimed to (i) identify the best soybean (Glycine max) cultivar for in vitro response for callus induction and plant regeneration; (ii) optimize genetic transformation of soybean mature cotyledon embryo via biobalistic. The callus induction and genetic transformation experiments were performed using IAS- 5, BR16 and MSOY6101 soybean cultivars. High auxin levels (5 mg/L 2,4- diclorofenoxiacetic, 5 mg/L 2-isopenteniladenine and 10 mg/L acid-naftalenacetic) were used for callus induction and to regeneration, adding 0,5 mg/L of acid-naftalenacetic and 0 to 2 mg/L of 6-benzylaminopurine (BAP), in all the treatments of regeneration. The following parameters were tested in order to optimize the transformation: tungsten concentration; BAP concentration (0 to 5 mg/L) in the co-cultivation medium and Kanamycin concentration in the selection medium. The genetic construction used was comprised of pCAMBIA2301 vector, containing gusA gene reporter, under the regulation of 35SCaMV constitutive promoter and NPTII selection gene that provides resistance to Kanamycin. Some differences were observed at the induction and regeneration of somatic embryogenesis among the three cultivars and only IAS-5 cultivar was selected for the assessment of embryogenic potential regarding plant differentiation and regeneration. This cultivar presented high level of callus induction, stable proliferation and stable regeneration. The results showed that the addition of 5 mg/L of 2,4D to the induction medium and 0,5 mg/L of BAP to the regeneration medium increased the levels of differentiation of the IAS-5 soybean embryos from 0 to 27,77%. Based on the results observed in the genetic transformation in N. benthamiana, 24 mg/μL of tungsten per membrane is now being used in the bombardment for the three soybean cultivars... (Complete abstract click electronic access below)

Orientador: Janete Aparecida Desidério<br>Coorientador: Sandra Helena Unêda Trevisoli<br>Banca: Newton Cavalcante de Noronha Junior<br>Banca: Sônia Marli Zingaretti<br>Resumo: Os objetivos do presente trabalho foram (i) identificar uma cultivar de soja (Glycine max) com melhor resposta in vitro para indução de calos e regeneração; (ii) otimizar o processo de transformação genética via biobalística em embriões oriundos de cotilédone maduros de soja. Os experimentos de indução de calos e transformação genética foram realizados com as cultivares IAS-5, BR16 e MSOY6101. Para indução de calos foram utilizadas altas doses de auxinas [5 mg/L 2,4D (2,4-diclorofenoxiacético- 2,4D), 5 mg/L 2ip (2-isopenteniladenina) e 10 mg/L ANA (ácido-naftalenacético)] e para regeneração de 0 a 2 mg/L de BAP (6-benzylaminopurine), adicionando-se 0,5 mg/L de ANA em todos os tratamentos. Vários parâmetros foram testados na transformação: concentração do tungstênio; BAP (0 a 5 mg/L) no co-cultivo e a concentração de canamicina para seleção de plantas. A construção utilizada foi constituída pelo vetor pCAMBIA 2301, contendo o gene repórter GUS, regulado pelo promotor constitutivo 35SCaMV e adição do gene de seleção NPTII que confere resistência a canamicina. Foram observadas diferenças para a indução e regeneração via embriogênese somática entre as cultivares de soja, resultando na seleção da cultivar 'IAS-5' para avaliação do potencial embriogênico quanto à diferenciação e regeneração de plantas. Essa cultivar apresentou alta taxa de indução, proliferação e regeneração estável. Os resultados mostraram que a adição de 0,5 mg/L de BAP ao meio de regeneração e 5 mg/L de 2,4D ao meio de indução aumentaram as taxas de diferenciação dos embriões de 0 para 27,77%. A partir dos resultados observados na transformação genética em N. benthamiana, adotou-se 24 mg/μL de tungstênio por membrana para as três cultivares de soja. Para regeneração em embrião maduro de soja o melhor resultado... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The present work aimed to (i) identify the best soybean (Glycine max) cultivar for in vitro response for callus induction and plant regeneration; (ii) optimize genetic transformation of soybean mature cotyledon embryo via biobalistic. The callus induction and genetic transformation experiments were performed using IAS- 5, BR16 and MSOY6101 soybean cultivars. High auxin levels (5 mg/L 2,4- diclorofenoxiacetic, 5 mg/L 2-isopenteniladenine and 10 mg/L acid-naftalenacetic) were used for callus induction and to regeneration, adding 0,5 mg/L of acid-naftalenacetic and 0 to 2 mg/L of 6-benzylaminopurine (BAP), in all the treatments of regeneration. The following parameters were tested in order to optimize the transformation: tungsten concentration; BAP concentration (0 to 5 mg/L) in the co-cultivation medium and Kanamycin concentration in the selection medium. The genetic construction used was comprised of pCAMBIA2301 vector, containing gusA gene reporter, under the regulation of 35SCaMV constitutive promoter and NPTII selection gene that provides resistance to Kanamycin. Some differences were observed at the induction and regeneration of somatic embryogenesis among the three cultivars and only IAS-5 cultivar was selected for the assessment of embryogenic potential regarding plant differentiation and regeneration. This cultivar presented high level of callus induction, stable proliferation and stable regeneration. The results showed that the addition of 5 mg/L of 2,4D to the induction medium and 0,5 mg/L of BAP to the regeneration medium increased the levels of differentiation of the IAS-5 soybean embryos from 0 to 27,77%. Based on the results observed in the genetic transformation in N. benthamiana, 24 mg/μL of tungsten per membrane is now being used in the bombardment for the three soybean cultivars... (Complete abstract click electronic access below)<br>Mestre

Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.<br>Includes bibliographical references (p. 195-209).<br>One of the main problems in mammalian cell culture systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. This inability translates into lower final protein titers and higher protein production and recovery costs. This thesis was undertaken to better understand the processes of death in CHO cells and to find ways to extend the viability of CHO cultures. A majority of CHO cells in culture were found to die by apoptosis, a genetically controlled form of cellular suicide. Protein synthesis inhibition in CHO cells led to rapid death, indicating that CHO cells were pre-disposed to death and that survival proteins needed to be continuously synthesized to protect cells from death. Caspases, a class of proteins found to be universally important in inducing apoptosis, were found to be activated in apoptotic CHO cells. Surprisingly, inhibition of caspase activity using z-VAD.fmk, a universal peptide inhibitor of caspases, failed to significantly extend viability in batch culture although it did prevent cleavage of known intracellular caspase substrates. In contrast, expression of bcl- 2, a well-characterized anti-apoptotic gene, was able to significantly increase the life of CHO batch cultures in response to both glucose limitation and growth factor withdrawal. Using these results, a pathway for apoptosis in CHO cells, focusing on the caspases and bcl-2, was suggested. An experiment was devised to statistically measure the ability of individual cells to replicate. Replication competence was found to correlate well with viability results from the acridine orange / ethidium bromide assay, but not with results from the trypan blue assay. These experiments proved that early apoptotic cells, which have lost membrane integrity but not chromatin integrity, can be considered dead since they lose the ability to replicate. In addition, the experiments proved that bcl-2 expression is able to extend the replication competence of cells under normal culture conditions. Bcl-2 expression was shown to improve both final product titers and integrated viable cell densities in CHO fed-batch cultures. It was also able to maintain insulin deprived fed-batch cultures in a viable and productive state for much longer than insulin supplemented cultures, thus suggesting an easy way to maintain viability and productivity coupled with slower growth. A slower growth rate has been reported in literature to yield higher product quality and productivity. Concentrations of insulin, a growth and survival factor used in CHO culture, were observed to drop rapidly in fed-batch cultures of CHO cells. The loss of insulin was concurrent with the accumulation of cells in the GO/G1 state and an increase in expression levels ofp53, a well-documented growth-inhibiting and apoptosis-inducing gene. Insulin degrading activity was found to be at least partially caused by release of proteases from cells into the culture medium. Insulin degradation was sharply reduced by adding sodium glycocholate, an amino-peptidase inhibitor, suggesting that amino-peptidases play a major role in insulin degradation in CHO fed-batch cultures. Based on the above results, possible ways to further reduce death and improve productivity in CHO cultures are also suggested.<br>by Joydeep Goswami.<br>Ph.D.

La diversité des espèces cultivées résulte d’une série d’évènements de domestication, de flux de gènes entre compartiments sauvages et cultivés, d’effets de la sélection adaptative naturelle et aussi de la sélection humaine et des dynamiques de diffusion à de larges échelles, souvent sur de longues périodes. L’impact de ces processus sur la diversité dépend non seulement de la biologie de l’espèce, mais elle est aussi fortement liée au contexte social et aux pratiques humaines. Cette thèse contribue à la compréhension de l’influence de l’Homme sur la dynamique de la diversité de l’amandier in situ. La démarche suivie fait appel à la génétique des populations et à l’anthropologie pour étudier la structuration de la diversité génétique de cette espèce fruitière pérenne allogame, cultivée au Liban. Cette étude comprend trois parties:Dans la première partie, nous avons cherché à comprendre les processus de diversification continue de l’amandier cultivée en se basant sur des évidences tirées de l'archéologie, de l'histoire et de la biologie évolutive de l’amandier dans le Bassin Méditerranéen. Nous avons utilisé une approche de génétique des populations avec de nombreux individus représentant chaque cultivar collecté auLiban. L'échantillonnage intensif de cultivars libanais a été comparé à un grand nombre d’arbres cultivés in situ provenant de différentes régions méditerranéennes. Les résultats nous ont permis de distinguer l’impact des différents périodes de diffusion sur la structure de la diversité génétique.La deuxième partie, a permis d’évaluer l’importance culturelle de l’amandier cultivé au Liban, et d’identifier sa diversité intra-spécifique telle qu’elle est perçue par les informateurs. Nos résultats montrent une hétérogénéité des connaissances des informateurs qui a mené à une taxonomie locale flexible. La flexibilité de la taxonomie locale est révélée par la présence de catégories englobantes et par la complexité du système de nomenclature.Dans la troisième partie, nous avons évalué l’effet du changement de pratiques de propagation sur la structuration et dynamique de la diversité génétique entre les variétés et à l’intérieur de chacune des deux variétés étudiées. Nos résultats montrent que le cultivar traditionnel, multiplié par semis, est structuré géographiquement. L’introduction du mode de propagation clonal par greffage a été adoptée graduellement. Au début, les agriculteurs ont maintenu une certaine diversité génétique par la multiplication sexuée occasionnelle du cultivar introduit. Par contre, l’introduction après l’adoption du greffage a réduit la diversité génétique intra-variétale dans les vergers récents.Cette thèse montre comment les connaissances et les décisions de l’Homme à différents échelles spatiales et temporelles influencent la structure et la dynamique de la diversité de cette espèce<br>The diversity of cultivated species results from a series of domestication events, gene flow between wild and cultivated compartments, effects of natural adaptive selection and also on human selection and diffusion dynamics at large scales, often over long periods. The impact of these processes on diversity depends not only on the biology of the species but is also strongly related to social context and human practices. This thesis contributes to the understanding of the influence of human on the dynamics of almond diversity in situ. The approach followed uses population genetics and anthropology to study the structuring of the genetic diversity of this allogamous perennial fruit species, grown in Lebanon. This study consists of three parts:In the first part, we sought to understand the processes of continuous diversification of cultivated almond trees based on evidence from archeology, history and evolutionary biology of almond trees in the Mediterranean Basin. We used a population genetics approach with many individuals representing each cultivar collected in Lebanon. Intensive sampling of Lebanese cultivars was compared to a large number of in situ grown trees from different Mediterranean regions. The results allowed us to distinguish the impact of different diffusion periods on the structure of genetic diversity.The second part assessed the cultural importance of the almond tree grown in Lebanon and identified its intraspecific diversity as perceived by the informants. Our results show heterogeneity of informants' knowledge that led to a flexible local taxonomy. The flexibility of local taxonomy is revealed by the presence of inclusive categories and the complexity of the nomenclature system.In the third part, we assessed the effect of the change in propagation practices on the structuration and dynamics of genetic diversity between two varieties and within each of the varieties studied.Our results show that the traditional cultivar, sexually propagated, is geographically structured. The introduction of clonal propagation mode by grafting was gradually adopted. At first, farmers maintained some genetic diversity through occasional sexual multiplication of the introduced cultivar. In contrast, introduction after grafting has reduced intra-varietal genetic diversity in recent orchards.This thesis shows how human knowledge and decisions at different spatial and temporal scales influence the structure and dynamics of the diversity of this species

In today's world of rapidly changing climates, ever-increasing human population growth and increased competition for space, the production of energy and high quality food to meet increasing demands presents an enormous challenge to the related industries. It has been predicted that global food production alone, must increase by 70% by 2050 to prevent worldwide starvation. The application of plant biotechnology offers a promising alternative over classical crop improvement techniques in the development of breeding programmes that could respond rapidly to future demands for high-yielding and nutritionally-enhanced crops. Adopting such strategies to underutilised legume crops represents a viable avenue for crop improvement, especially in developing countries with a high incidence of poverty and malnutrition, where local environmental conditions frequently prove too challenging for effective cultivation of commercial cultivars. The main objective of this thesis was to generate fundamental data regarding the adoption of tissue culture-based transformation strategies for the genetic improvement of two such crops, jicama [Pachyrhizus erosus (L.) Urban] and Bambara groundnut [Vigna subterranea (L.) Verdc.]. The work carried out on jicama investigated the regeneration potential of this plant in vitro as a basis for future biotechnological techniques. Preliminary tissue culture experiments identified explant types and plant growth regulator (PGR) matrices and concentrations that produced optimal regenerative responses in vitro. Histological analysis of regenerated tissue revealed that shoot formation occurred via somatic embryogenesis. Possible avenues for further optimisation of the explant-to-plant regeneration protocol, for downstream molecular genetic applications, were explored and discussed. For Bambara groundnut, this study initially focused on developing a novel explant-to-plant regeneration system, previously not reported for landraces DipC, Uniswa-Red and S 19-3, through the adoption and modification of techniques specific for distinct Bambara landraces. This micropropagation protocol employed whole zygotic embryos as starting explants, which formed the basis for subsequent transformation studies. Genetic transformation was attempted via biolistic- and Agrobacterium-mediated approaches, using transformation vectors pVDH65, pB1l21, harbouring the nptII selectable marker and uidA (gus) reporter genes, while pBIl21-LeB4-Ber e I additionally carried the methionine-rich ber el gene. T-DNA transfer in transformed explants was confirmed via histochemical GUS analyses, while peR assays identified putatively transformed regenerated shoots. Explant viability was significantly compromised following bombardment or inoculation, resulting in reduced shoot regeneration, and thus limited the production of transgenic plants. Improvements in transformation efficiency and regeneration frequency were made following protocol optimisation, and the potential for further improvements to efficiently produce well-developed, healthy transgenic shoots were explored and discussed. The results presented in this thesis describe the optimisation of innovative tissue culture and regeneration protocols, which form a fundamental prerequisite to future transformation-based experiments for jicama and Bambara groundnut. Additionally, previously unpublished data generated from transformation studies in Bambara groundnut has revealed its potential for genetic manipulation. These results have established the foundation of novel micropropagation and transformation systems with the potential to be further optimised to genetically enhance and exploit the enormous agronomic potential of these underutilised legume crops.

This thesis analyses the struggle over rights to benefits and ownership of plant genetic resources and the global regime complex on the management of plant genetic resources, and how different regimes concerning these resources cooperate or stand in opposition to each other. Because of changes in US patent law and the establishment of TRIPS, patent claims over plant genetic resources has increased dramatically globally. This, amongst other things, in turn has lead to the acrimonious negotiations of access and benefit sharing arrangements within the framework CBD. The objective of this thesis is to examine the interaction between the international regimes regulating genetic resources and intellectual property and to analyse how these regime interactions, affect the protection of traditional knowledge held by local communities, indigenous peoples and small farmers in developing countries. The thesis concludes that it exists several regime interactions that are disruptive and undermine the possibility of protecting traditional knowledge from misappropriation. It is further concluded that modifications of the existing IPR regimes, on the disclosure of inventions, with a certificate of legal provenance, securing FPIC, MAT and benefit sharing, may serve as one brick in the wall that protect traditional knowledge from misappropriation through wrongly granted patents. But a certificate of legal provenance will not do the work alone. To protect traditional knowledge associated with genetic resources in the long term bio-cultural solutions which sustains the entire community where traditional knowledge is embedded is needed.