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Journal articles on the topic 'Culture affinity'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Garle, Michael J., and Jeffrey R. Fry. "A Comparison of Hepatic Enzyme Activities and their Modulation by Dexamethazone in Freshly Isolated and Cultured Hepatocytes and in the Differentiated Hepatoma Cell Line, 2sFou." Alternatives to Laboratory Animals 24, no. 1 (1996): 31–37. http://dx.doi.org/10.1177/026119299602400106.

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Rodent hepatocytes are mitotically inhibited and lose hepatospecific functions over time in culture. In contrast, some differentiated hepatoma cell lines express stable hepatospecific functions in culture, but at much lower levels than those initially found in primary hepatocytes. A number of hepatospecific functions were measured in freshly isolated and cultured rat hepatocytes; these were compared to activities found in the differentiated Reuber hepatoma cell line, 2sFou. The effects of dexamethazone on these activities were also investigated, since dexamethazone is reported to enhance the e

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2

Smith, John. "The trinitarian dance with culture: Trinity as the missiological optic for understanding culture." Missiology: An International Review 48, no. 2 (2020): 154–68. http://dx.doi.org/10.1177/0091829619887386.

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In discussions of imago Dei, we largely confine ourselves to functional and moral affinity to God, that is that we have been made in God’s image with the result that obedience to God amounts to imitating his moral character and behavior. However, we need to add the existential affinity of God’s being to our understanding of God’s image in us, specifically the fact that he is both singular (in essence) and plural (in person). To some degree, people share God’s singular–plural quality and that one–many affinity can give great insight into gospel ministry. Moreover, as with the moral dimension of

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3

Roos, Joseph W., and Martin A. Hjortso. "Control of anEscherichia colimixed culture via affinity binding." Biotechnology and Bioengineering 38, no. 4 (1991): 380–88. http://dx.doi.org/10.1002/bit.260380408.

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4

Suranovic, Steven, and Robert Winthrop. "Trade Liberalization and Culture." Global Economy Journal 14, no. 1 (2014): 57–78. http://dx.doi.org/10.1515/gej-2013-0047.

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This paper addresses the effect of international trade on cultural outcomes from both economic and anthropological perspectives. Definitions of culture are informed by anthropology and then incorporated into a standard economic trade models in two distinct ways. In the “cultural affinity from work” model, workers receive a non-pecuniary cultural benefit from work in a particular industry. In the “cultural externality” model, consumers of a product receive utility from other consumer’s consumption of a domestic good. We show that resistance to change due to cultural concerns can reduce the nati

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Dickman, K. G., and J. L. Renfro. "Primary culture of flounder renal tubule cells: transepithelial transport." American Journal of Physiology-Renal Physiology 251, no. 3 (1986): F424—F432. http://dx.doi.org/10.1152/ajprenal.1986.251.3.f424.

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Renal proximal tubule cells from the winter flounder (Pseudopleuronectes americanus) were maintained in a functionally differentiated state for up to 16 days in primary culture on floating collagen gels. The cells were confluent after 7-8 days in culture, contracted the collagen gels, and exhibited ciliary activity. Electron microscopy indicated that the cultures were composed of continuous sheets of columnar epithelial cells that had established structural polarity. When mounted in Ussing chambers, the cultures exhibited a small mucosa-negative potential difference (0.6 +/- 0.10 mV) and a low

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6

van Dijk, H. P., M. J. Kroos, J. S. Starreveld, et al. "Expression of haemopexin receptors by cultured human cytotrophoblast." Biochemical Journal 307, no. 3 (1995): 669–72. http://dx.doi.org/10.1042/bj3070669.

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The expression of cell-surface haemopexin (Hx) receptors on human cytotrophoblasts was assessed by using four different Hx species purified from plasma: human Hx isolated by wheatgerm-affinity chromatography, human Hx isolated by haem-agarose-affinity chromatography and rabbit and rat Hx, also isolated by haem-agarose-affinity chromatography. About 3500-7000 high-affinity (Kd 0.34-0.85 nM) receptors per cell were measured by Scatchard-type analysis at 4 degrees C using human (species obtained by both methods) or rabbit 125I-labelled haem-Hx. Measured simultaneously, transferrin receptor number

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Eckel, J., G. van Echten, and H. Reinauer. "Adult cardiac myocytes in primary culture: cell characteristics and insulin-receptor interaction." American Journal of Physiology-Heart and Circulatory Physiology 249, no. 2 (1985): H212—H221. http://dx.doi.org/10.1152/ajpheart.1985.249.2.h212.

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Calcium-tolerant adult cardiac myocytes were kept in culture under serum-free conditions in the presence of physiological concentrations of insulin. Up to 4 days, 70% of cells retained their in vivo rodshaped morphology without gross structural alterations. During that period a constant ATP-to-ADP ratio was observed with a mean value of 10.6 +/- 0.5 (n = 4). The rate of [14C]phenylalanine incorporation remained unaltered up to 63 h in culture. Insulin binding to cultured cells was found to be time-and temperature-dependent, reversible, and highly specific. Scatchard analysis of equilibrium bin

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8

Sinclair, P. R., W. J. Bement, N. Gorman, H. H. Liem, A. W. Wolkoff, and U. Muller-Eberhard. "Effect of serum proteins on haem uptake and metabolism in primary cultures of liver cells." Biochemical Journal 256, no. 1 (1988): 159–65. http://dx.doi.org/10.1042/bj2560159.

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A role of haemopexin in transporting haem to hepatocytes for degradation has been inferred from the high affinity of haemopexin for haem. We have examined this question in primary cultures of chick-embryo and adult rat liver cells. We present here the results of four sets of experiments which indicate that haemopexin retarded haem uptake by hepatocytes in culture. (1) Haem bound to bovine serum albumin is known to repress the activity of delta-aminolaevulinate synthase in chick cultures as indicated by decreased porphyrin accumulation. When haem-albumin was added in the presence of excess puri

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9

WEXLER, ERIC M., OKSANA BERKOVICH, and SCOTT NAWY. "Role of the low-affinity NGF receptor (p75) in survival of retinal bipolar cells." Visual Neuroscience 15, no. 2 (1998): 211–18. http://dx.doi.org/10.1017/s095252389815201x.

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We have examined the role of neurotrophins in promoting survival of mammalian rod bipolar cells (RBC) in culture. Retinas taken from 8- to 10-day-old Long-Evans rats were dissociated and cultured in media supplemented with either nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), or basic fibroblast growth factor (FGF-2). Survival was measured by the number of cells that were immunoreactive for α-, β-, γ-PKC, a bipolar cell-specific marker. Compared to untreated cultures, CNTF had no effect on RBC survival, while NGF

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10

Wang, Guang Jian, Hye Joo Chung, Jamie Schnuer, et al. "High Affinity Glutamate Transport in Rat Cortical Neurons in Culture." Molecular Pharmacology 53, no. 1 (1998): 88–96. http://dx.doi.org/10.1124/mol.53.1.88.

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11

Saenz de Tejada, I., M. P. Carson, A. de las Morenas, I. Goldstein, and A. M. Traish. "Endothelin: localization, synthesis, activity, and receptor types in human penile corpus cavernosum." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 4 (1991): H1078—H1085. http://dx.doi.org/10.1152/ajpheart.1991.261.4.h1078.

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The localization, synthesis, and activity of endothelin and the receptor types mediating its effects in penile corpus cavernosum were investigated in whole tissue and in cultured cells derived from this tissue. With immunocytochemistry, utilizing an antiendothelin 1 (ET-1) monoclonal antibody, endothelin-like immunoreactivity was localized intensely in the endothelium and to a lesser degree in the trabecular smooth muscle. Human corpus cavernosum endothelial cells in culture expressed preproendothelin 1 mRNA, as determined by Northern blot analysis. Significant amounts of endothelin-like immun

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Rameezdeen, Raufdeen, and Nishanthi Gunarathna. "Organisational Culture in Construction: An Employee Perspective." Construction Economics and Building 3, no. 1 (2012): 19–30. http://dx.doi.org/10.5130/ajceb.v3i1.2908.

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A large number of stakeholders in construction projects makes the construction industry prone to disputes. The historical separation between design and construction add to this phenomenon by having a consultant for design and a contractor for construction. Communication breakdown, frequently, is the first sign of problems, notably in the relationship between the Contractor and the Consultant. Therefore, it appears that the split between design and construction has given rise to two separate cultures in the construction industry. This paper attempts to identify whether there is a difference in

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Ennes, H. S., J. A. McRoberts, P. E. Hyman, and W. J. Snape. "Characterization of colonic circular smooth muscle cells in culture." American Journal of Physiology-Gastrointestinal and Liver Physiology 263, no. 3 (1992): G365—G370. http://dx.doi.org/10.1152/ajpgi.1992.263.3.g365.

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The receptor-binding properties of isolated rabbit colonic circular smooth muscle cells in primary culture have been investigated. In intact smooth muscle, acetylcholine, acting through M2 muscarinic receptors, and vasoactive intestinal polypeptide (VIP), acting through VIP receptors, are two of the principal neurotransmitters mediating contraction and relaxation, respectively. The muscarinic receptor was present in very high levels (600,000 receptors/cell) on freshly isolated colonic smooth muscle cells as shown by binding of the muscarinic receptor antagonist N-methylscopolamine (NMS). Howev

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Alexander, Samuel M., and Bryan M. Gebhardt. "Rapid Immunoassay for the Detection of Genital Herpes Infection." Infectious Diseases in Obstetrics and Gynecology 2, no. 1 (1994): 30–33. http://dx.doi.org/10.1155/s1064744994000360.

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Objective:We evaluated a new affinity membrane strip test for the diagnosis of herpetic genital infections. Test strip results, which are available by immunoassay in 30 min without the need for special equipment, were compared with the results of viral culture.Methods:Twenty-eight female patients with vulvar lesions thought to be due to genital herpes simplex virus (HSV) infection were tested. The affinity membrane strip was applied to the genital lesion. Dacron swabs were then applied to the lesions and the swab contents cultured for HSV. For the immunoassay, the test strip was immersed in pe

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15

Boynton, Anthony Dwayne. "August Wilson, Afrofuturism, & Gem of the Ocean." Open Cultural Studies 2, no. 1 (2018): 374–82. http://dx.doi.org/10.1515/culture-2018-0034.

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Abstract August Wilson's Century Cycle is as much a theatrical experiment of black cultural history and sociology as it is one of storytelling. Though often considered a realist playwright, Wilson walks beyond the realist landscape into speculative and imagined ones in Gem of the Ocean. His investment in cultural critique and history enhances the possibility of an enriching analysis of his work as speculative fiction. This research project locates the ties between Wilson’s affinity with history and the creation of a dystopian Pittsburgh in the play. In Wilson’s work, set in 1904, the antebellu

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16

Dunfield, Peter F., Werner Liesack, Thilo Henckel, Roger Knowles, and Ralf Conrad. "High-Affinity Methane Oxidation by a Soil Enrichment Culture Containing a Type II Methanotroph." Applied and Environmental Microbiology 65, no. 3 (1999): 1009–14. http://dx.doi.org/10.1128/aem.65.3.1009-1014.1999.

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ABSTRACT Methanotrophic bacteria in an organic soil were enriched on gaseous mixing ratios of <275 parts per million of volume (ppmv) of methane (CH4). After 4 years of growth and periodic dilution (>1020 times the initial soil inoculum), a mixed culture was obtained which displayed an apparent half-saturation constant [Km(app) ] for CH4 of 56 to 186 nM (40 to 132 ppmv). This value was the same as that measured in the soil itself and about 1 order of magnitude lower than reported values for pure cultures of methane oxidizers. However, theKm(app) increased when the culture was transferred

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17

Woo, Benjamin. "Cultural studies and actually existing culture." International Journal of Cultural Studies 23, no. 3 (2020): 310–16. http://dx.doi.org/10.1177/1367877920903053.

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The cultural studies tradition is a big tent that is defined by academic realpolitik and feelings of affinity or disaffinity as much as anything else. This article recounts my own introduction to and professionalization within the field of cultural studies and, more particularly, how my relationship to empirical research methods changed over time. I ultimately want to argue for the importance of staying grounded in the analysis of real people’s real experiences of culture.

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18

Kowalczyk, AP, RH Tulloh, and PJ McKeown-Longo. "Polarized fibronectin secretion and localized matrix assembly sites correlate with subendothelial matrix formation." Blood 75, no. 12 (1990): 2335–42. http://dx.doi.org/10.1182/blood.v75.12.2335.2335.

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Abstract Endothelial cells in vivo form the interface between the vascular and interstitial compartments and are strategically located to mediate vascular permeability and hemostasis. One mechanism endothelial cells use to maintain a nonthrombogenic surface is to polarize basement membrane constituents to the basolateral cell surface. In the present study, we began characterization of the mechanisms used by endothelial cells in the assembly of a subcellular fibronectin matrix. Immunofluorescence microscopy was used to localize extracellular matrix fibronectin in endothelial cell cultures. In c

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Kowalczyk, AP, RH Tulloh, and PJ McKeown-Longo. "Polarized fibronectin secretion and localized matrix assembly sites correlate with subendothelial matrix formation." Blood 75, no. 12 (1990): 2335–42. http://dx.doi.org/10.1182/blood.v75.12.2335.bloodjournal75122335.

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Endothelial cells in vivo form the interface between the vascular and interstitial compartments and are strategically located to mediate vascular permeability and hemostasis. One mechanism endothelial cells use to maintain a nonthrombogenic surface is to polarize basement membrane constituents to the basolateral cell surface. In the present study, we began characterization of the mechanisms used by endothelial cells in the assembly of a subcellular fibronectin matrix. Immunofluorescence microscopy was used to localize extracellular matrix fibronectin in endothelial cell cultures. In contrast t

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20

von Cramon-Taubadel, Noreen, and Stephen J. Lycett. "Assessing the relative impact of historical divergence and inter-group transmission on cultural patterns: a method from evolutionary ecology." Philosophical Transactions of the Royal Society B: Biological Sciences 373, no. 1743 (2018): 20170054. http://dx.doi.org/10.1098/rstb.2017.0054.

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In the study of cultural evolution, observed among-group affinity patterns reflect the effects of processes such as mutation (e.g. innovation and copying error), between-group interaction (culture flow), drift and selection. As in biology, cultural affinity patterns are often spatially correlated, making it difficult to distinguish between the opposing geographically mediated forces of divergence and interaction, which cause groups to become more distinct or similar over time, respectively. Analogous difficulties are faced by evolutionary biologists examining the relationship between biologica

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Schwark, Sebastian, Wei Sun, Jörg Stute, Dirk Lütkemeyer, Mathias Ulbricht, and Börje Sellergren. "Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes." RSC Advances 6, no. 58 (2016): 53162–69. http://dx.doi.org/10.1039/c6ra06632a.

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22

Boyer, Gregory L., Stacie A. Kane, Jeffrey A. Alexander, and Dallas B. Aronson. "Siderophore formation in iron-limited cultures of Frankia sp. strain 52065 and Frankia sp. strain CeSI5." Canadian Journal of Botany 77, no. 9 (1999): 1316–20. http://dx.doi.org/10.1139/b99-064.

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Frankia sp. strain 52065 (DDB 03010210) produces a high-affinity iron chelator or siderophore termed frankobactin to obtain iron needed for nitrogen fixation under iron-limiting conditions. Cultures of Frankia sp. strain 52065 and Frankia sp. strain CeSI5 (UFG 026605) were grown under iron-limiting and iron-replete conditions and examined for siderophore formation throughout the growth cycle using the HPLC 55Fe-binding assay and the Csaky chemical assay. Both cultures produced frankobactin under iron-limiting, but not iron-replete, conditions. This is the first positive report of hydroxamate s

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Vančová, Terézia, and Luboš Střelec. "Consumption Function in the Context of Cultural Affinity Zones." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 68, no. 4 (2020): 797–806. http://dx.doi.org/10.11118/actaun202068040797.

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Consumers' purchasing behaviour is affected at the microeconomic level by personal, psychological, situational, social and cultural factors. Beside the political and economic factors, culture with its beliefs, values, attitudes and traditions plays a substantial role also at the macroeconomic level in affecting national aggregate consumption, despite the recent phenomenon of globalisation. There is an evidence of excess sensitivity in European countries, which confirms that income change is a good predictor of consumption change. Clusters of European countries constructed according to single c

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Vuk-Pavlovic, Z., K. Pavelic, and S. Vuk-Pavlovic. "Modulation of in vitro growth of murine myeloid leukemia by an autologous substance immunochemically cross-reactive with insulin and antiinsulin serum." Blood 67, no. 4 (1986): 1031–35. http://dx.doi.org/10.1182/blood.v67.4.1031.1031.

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Abstract Murine myeloid leukemia secretes a substance immunochemically cross- reactive with insulin (SICRI) both in vivo and in serum-free media. High SICRI concentrations in peripheral blood of tumorous animals do not affect circulating glucose levels. In culture, DNA synthesis rate per leukemic cell is proportional to cell density and is reduced by antiinsulin serum. Culture medium conditioned by leukemia cells as well as SICRI affinity purified from this medium stimulate DNA synthesis in cultured leukemia cells. It appears that autocrine stimulation of murine myeloid leukemia can be mediate

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Vuk-Pavlovic, Z., K. Pavelic, and S. Vuk-Pavlovic. "Modulation of in vitro growth of murine myeloid leukemia by an autologous substance immunochemically cross-reactive with insulin and antiinsulin serum." Blood 67, no. 4 (1986): 1031–35. http://dx.doi.org/10.1182/blood.v67.4.1031.bloodjournal6741031.

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Murine myeloid leukemia secretes a substance immunochemically cross- reactive with insulin (SICRI) both in vivo and in serum-free media. High SICRI concentrations in peripheral blood of tumorous animals do not affect circulating glucose levels. In culture, DNA synthesis rate per leukemic cell is proportional to cell density and is reduced by antiinsulin serum. Culture medium conditioned by leukemia cells as well as SICRI affinity purified from this medium stimulate DNA synthesis in cultured leukemia cells. It appears that autocrine stimulation of murine myeloid leukemia can be mediated in part

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26

Moody, Stephanie. "Bullies and blackouts: Examining the participatory culture of online book reviewing." Convergence: The International Journal of Research into New Media Technologies 25, no. 5-6 (2017): 1063–76. http://dx.doi.org/10.1177/1354856517721805.

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This article examines online book reviewing practices through Henry Jenkins’s notion of ‘participatory culture’ and illustrates the power dynamics and market pressures that shape this participation. While the individuals featured in this article participate in shared affinity spaces around a passion for reading and writing books, they also participate in a publishing industry increasingly reliant on reviews and ratings. I argue that the sabotaging and bullying of authors and reviewers, and the power dynamics reinforced through these tactics, risk being occluded by scholarship that emphasizes t

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27

Heine, Thomas, Marika Mehnert, Rïngo Schwabe, and Dirk Tischler. "Siderophore Purification via Immobilized Metal Affinity Chromatography." Solid State Phenomena 262 (August 2017): 505–8. http://dx.doi.org/10.4028/www.scientific.net/ssp.262.505.

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Siderophores are low-molecular weight compounds that are produced by organisms to assimilate vital Fe3+ out of iron-deficient environments. They are of interest for several (bio-) technological applications because of their high selectivity for several metal ions. Unfortunately, the concentration in supernatants is often low and thus it is challenging to purify or even enrich these compounds. We applied different types of siderophores onto an immobilized metal-resin that was loaded with either Ni2+, Co2+ or Fe3+. Elution was done with ethanol to reduce salt load and facilitate downstream proce

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Landschulz, KT, AN Noyes, O. Rogers, and SH Boyer. "Erythropoietin receptors on murine erythroid colony-forming units: natural history." Blood 73, no. 6 (1989): 1476–86. http://dx.doi.org/10.1182/blood.v73.6.1476.1476.

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Abstract Erythropoietin (Epo) response and binding was assessed in purified murine CFU-E and their descendants. Several features emerged. First, Epo on CFU-E is in rapid flux: Half-time for 125I-Epo internalization is approximately four to five minutes. Second, computer-aided Scatchard analyses indicate that greater than 70 high-affinity Epo-receptor sites on anemic animal CFU-E are sometimes already occupied by Epo acquired in vivo. When this is removed, 40% of greater than or equal to 370 sites per CFU-E belong to a high-affinity class (dissociation constant, kd: 73 pmol/L +/- 15 [SE]) and 6

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Landschulz, KT, AN Noyes, O. Rogers, and SH Boyer. "Erythropoietin receptors on murine erythroid colony-forming units: natural history." Blood 73, no. 6 (1989): 1476–86. http://dx.doi.org/10.1182/blood.v73.6.1476.bloodjournal7361476.

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Erythropoietin (Epo) response and binding was assessed in purified murine CFU-E and their descendants. Several features emerged. First, Epo on CFU-E is in rapid flux: Half-time for 125I-Epo internalization is approximately four to five minutes. Second, computer-aided Scatchard analyses indicate that greater than 70 high-affinity Epo-receptor sites on anemic animal CFU-E are sometimes already occupied by Epo acquired in vivo. When this is removed, 40% of greater than or equal to 370 sites per CFU-E belong to a high-affinity class (dissociation constant, kd: 73 pmol/L +/- 15 [SE]) and 60% belong

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Baranowski, Eric, Noemi Sevilla, Nuria Verdaguer, Carmen M. Ruiz-Jarabo, Ewald Beck, and Esteban Domingo. "Multiple Virulence Determinants of Foot-and-Mouth Disease Virus in Cell Culture." Journal of Virology 72, no. 8 (1998): 6362–72. http://dx.doi.org/10.1128/jvi.72.8.6362-6372.1998.

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ABSTRACT Hypervirulent variants of foot-and-mouth disease virus (FMDV) of serotype C arise upon serial cytolytic or persistent infections in cell culture. A specific mutation in the internal ribosome entry site of persistent FMDV was previously associated with enhanced translation initiation activity that could contribute to the hypervirulent phenotype for BHK-21 cells. Here we report that several hypervirulent FMDV variants arising upon serial cytolytic passage show an invariant internal ribosome entry site but have a number of mutations affecting structural and nonstructural viral proteins.

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Zhang, Ci, Frank Vago, Fei Guo, et al. "Affinity Cryo-Electron Microscopy Studies of Viral Particles Captured Directly From Cell Culture." Microscopy and Microanalysis 21, S3 (2015): 547–48. http://dx.doi.org/10.1017/s1431927615003530.

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Barros, Daniela, Eduardo Conde-Sousa, Andreia M. Gonçalves, et al. "Engineering hydrogels with affinity-bound laminin as 3D neural stem cell culture systems." Biomaterials Science 7, no. 12 (2019): 5338–49. http://dx.doi.org/10.1039/c9bm00348g.

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Kayo, Sabrina, Janina Bahnemann, Matthias Klauser, Ralf Pörtner, and An-Ping Zeng. "A microfluidic device for immuno-affinity-based separation of mitochondria from cell culture." Lab on a Chip 13, no. 22 (2013): 4467. http://dx.doi.org/10.1039/c3lc50739d.

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Nakano, I., C. Z. Soe, and R. Codd. "Isolation of doxorubicin from a bacterial culture using immobilised metal ion affinity chromatography." RSC Advances 5, no. 58 (2015): 46437–42. http://dx.doi.org/10.1039/c5ra07639k.

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Doxorubicin was isolated as a free ligand from aStreptomyces peucetiusvar.caesiusculture using Ni(ii)-based IMAC. This easy-to-use, water-compatible method could improve the security of doxorubicin supply.

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Reynolds, Richard, Christine Steffen, and Norbert Herschkowitz. "High-affinity uptake of ?-[3H]aminobutyric acid by isolated mouse oligodendrocytes in culture." Neurochemical Research 12, no. 10 (1987): 885–90. http://dx.doi.org/10.1007/bf00966310.

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Jennings, Charles G. B., and Anne W. Mudge. "Chick myotubes in culture express high-affinity receptors for calcitonin gene-related peptide." Brain Research 504, no. 2 (1989): 199–205. http://dx.doi.org/10.1016/0006-8993(89)91357-7.

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Stuart, Rebekah B., Suzanne Zwaanswijk, Neil D. MacKintosh, Boontarikaan Witikornkul, Peter M. Brophy, and Russell M. Morphew. "The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica." Parasitology Research 120, no. 3 (2021): 979–91. http://dx.doi.org/10.1007/s00436-021-07055-5.

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AbstractFasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelmintics; with only triclabendazole (TCBZ) currently effective against all stages of F. hepatica in livestock and humans. There is widespread resistance to TCBZ and its detoxification by flukes might contribute to the mechanism. However, there is limited phase I capacity in adult parasitic helminths with the phase II detoxification system dominated by t

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Zhang, Shiquen, Baoman Li, Ditte Lovatt, et al. "5-HT2B receptors are expressed on astrocytes from brain and in culture and are a chronic target for all five conventional ‘serotonin-specific reuptake inhibitors’." Neuron Glia Biology 6, no. 2 (2010): 113–25. http://dx.doi.org/10.1017/s1740925x10000141.

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In well-differentiated primary cultures of mouse astrocytes, which express no serotonin transporter (SERT), the ‘serotonin-specific reuptake inhibitor’ (SSRI) fluoxetine leads acutely to 5-HT2B receptor-mediated, transactivation-dependent phosphorylation of extracellular regulated kinases 1/2 (ERK1/2) with an EC50 of ~5 μM, and chronically to ERK1/2 phosphorylation-dependent upregulation of mRNA and protein expression of calcium-dependent phospholipase A2 (cPLA2) with ten-fold higher affinity. This affinity is high enough that fluoxetine given therapeutically may activate astrocytic 5-HT2B rec

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Yue, G., P. Hu, Y. Oh, et al. "Culture-induced alterations in alveolar type II cell Na+ conductance." American Journal of Physiology-Cell Physiology 265, no. 3 (1993): C630—C640. http://dx.doi.org/10.1152/ajpcell.1993.265.3.c630.

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Changes in Na+ transport in rat alveolar type II (ATII) cells during culture were quantified and related to alterations in spatial distribution of proteins antigenically related to amiloride-sensitive Na+ channels. Adult rat ATII cells were cultured for periods ranging from 24 to 96 h. When patch clamped in the whole cell mode, both freshly isolated and cultured ATII cells exhibited outwardly rectified Na+ currents. At 0 and 24 h in culture, these currents were equally inhibited by amiloride, benzamil, and 5-(N-ethyl-N-isopropyl)-2',4'-amiloride (inhibitory constant approximately 1 microM). Th

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Sawyer, ST, SB Krantz, and K. Sawada. "Receptors for erythropoietin in mouse and human erythroid cells and placenta." Blood 74, no. 1 (1989): 103–9. http://dx.doi.org/10.1182/blood.v74.1.103.103.

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Abstract High and lower affinity receptors for erythropoietin (EP) were initially identified on a very pure population of EP-responsive erythroblasts obtained from the spleens of mice infected with anemia strain of Friend virus (FVA). The structure of the receptor for EP in these cells was determined to be proteins of 100 and 85 Kd by cross- linking 125I-EP. In this investigation, studies on the receptors for EP were extended to other mouse erythroid cells and human erythroid cells as well as to the placentas of mice and rats. Only lower affinity receptors for EP were detected on erythroblasts

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Sawyer, ST, SB Krantz, and K. Sawada. "Receptors for erythropoietin in mouse and human erythroid cells and placenta." Blood 74, no. 1 (1989): 103–9. http://dx.doi.org/10.1182/blood.v74.1.103.bloodjournal741103.

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High and lower affinity receptors for erythropoietin (EP) were initially identified on a very pure population of EP-responsive erythroblasts obtained from the spleens of mice infected with anemia strain of Friend virus (FVA). The structure of the receptor for EP in these cells was determined to be proteins of 100 and 85 Kd by cross- linking 125I-EP. In this investigation, studies on the receptors for EP were extended to other mouse erythroid cells and human erythroid cells as well as to the placentas of mice and rats. Only lower affinity receptors for EP were detected on erythroblasts purified

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Kajiyama, Y., та M. Ui. "Switching from α1- to β-subtypes in adrenergic response during primary culture of adult-rat hepatocytes as affected by the cell-to-cell interaction through plasma membranes". Biochemical Journal 303, № 1 (1994): 313–21. http://dx.doi.org/10.1042/bj3030313.

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The alpha 1-adrenergic response was predominant over the beta-adrenergic one in adult rat hepatocytes, when the responses were measured as the agonist-induced generations of Ins(1,4,5)P3 and cyclic AMP, respectively. During primary culture of the adult rat hepatocytes, the beta-adrenergic response developed rapidly, whereas the alpha 1-response decreased gradually. Such receptor-subtype switching did not occur unless the cells were cultured under conditions favourable for cell growth, i.e. at low cell density (10(4) cells/cm2). The switching was prevented progressively as the cell culture dens

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Dattamajumdar, Satarupa. "KINSHIP TERMINOLOGY IN LEPCHA." Buckingham Journal of Language and Linguistics 3 (September 16, 2010): 179–86. http://dx.doi.org/10.5750/bjll.v3i0.29.

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Lepcha being a language of the Tibeto Burman language family exhibit structural traits of the of the kinship terminological system realised in the Indian subcontinent. Kinship terminology has been analysed by different scholars from different points of view like, generation, sex, affinity, collaterality, relative age, polarity, affinity, etc. The present paper examines the Lepcha kinship terminology keeping the existing structural criteria in view along with culture and language specific aspects into consideration.

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Overend, C., R. Mitchell, D. He, G. Rompato, M. J. Grubman, and A. E. Garmendia. "Recombinant swine beta interferon protects swine alveolar macrophages and MARC-145 cells from infection with Porcine reproductive and respiratory syndrome virus." Journal of General Virology 88, no. 3 (2007): 925–31. http://dx.doi.org/10.1099/vir.0.82585-0.

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Swine beta interferon (swIFN-β) produced in HEK 293 cells infected with a recombinant, replication-defective human adenovirus 5 (Ad5) encoding the swIFN-β gene was tested for antiviral activity against Porcine reproductive and respiratory syndrome virus (PRRSV). MARC-145 cells were incubated overnight with dilutions of supernatant fluids from HEK 293 cells infected with Ad5-swIFN-β or with an Ad5 control virus (Ad5-Blue). Treated cells were infected with PRRSV; MARC-145 cells incubated with Ad5-Blue supernatants developed cytopathic effects (CPE), whereas those incubated with swIFN-β showed no

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VINSON, DUNCAN. "Liberal Religion, Artistic Autonomy, and the Culture of Secular Choral Societies." Journal of the Society for American Music 4, no. 3 (2010): 339–67. http://dx.doi.org/10.1017/s1752196310000179.

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AbstractU.S. choral societies typically formalize themselves as secular organizations analogous to symphony orchestras and opera companies. Yet choral societies differ from symphonic or operatic organizations because almost all choruses depend on volunteer singers. Part of what attracts singers into choruses is a sometimes unacknowledged affinity between the religious traditions of liberal Christians and Jews and the culture of choral singing as practiced in formally secular choral societies. The liberal tradition in religion encourages a habitus toward music that might be called a “sense of l

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Benyajati, Siribhinya, and J. Larry Renfro. "Taurine secretion in primary monolayer cultures of flounder renal epithelium: stimulation by low osmolality." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 279, no. 2 (2000): R704—R712. http://dx.doi.org/10.1152/ajpregu.2000.279.2.r704.

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Transepithelial taurine fluxes determined in short-circuited monolayer cultures of flounder renal proximal cells in Ussing chambers revealed net taurine secretion. Both unidirectional secretory and reabsorptive taurine fluxes exhibited saturation kinetics contributed by two distinct saturable transepithelial taurine transport systems operating at different taurine concentration ranges. The taurine secretory system operating below 0.5 mM had lower affinity but higher capacity than the reabsorptive system, whereas the one operating at high concentrations (0.5–3.0 mM) had higher affinity but the

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Budd, Tracy J., Frank W. Hemming, and Bruce Middleton. "Characterization of the LDL receptor in rat promegakaryoblasts in culture." Bioscience Reports 11, no. 1 (1991): 15–21. http://dx.doi.org/10.1007/bf01118601.

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Rat promegakaryoblasts (RPM, a precursor platelet cell line) in culture exhibited a capacity to bind, take up and degrade125I-LDL. The low density lipoprotein (LDL) binding showed the following characteristics: (a) high affinity, (b) saturability, (c) specificity, (d) down-regulation, after exposure to 25 hydroxycholesterol. Furthermore the proteolytic degradation of125I-LDL by RPMs was inhibited by chloroquine which interferes with the lysosomal degradation processes. These findings show LDL receptor cell biology of RPM to be of the classical type and to differ from that of platelets.

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Pusch, D., St Ihle, I. Graeber, J. M. López-Pila, and M. Lebuhn. "Quantitative detection of enteroviruses in activated sludge by cell culture and real-time RT-PCR using paramagnetic capturing." Journal of Water and Health 3, no. 3 (2005): 313–24. http://dx.doi.org/10.2166/wh.2005.039.

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We have compared in extracts of activated sludge the number of enteroviruses detectable with buffalo green monkey (BGM) cell-cultures versus the number of enteroviral genomes determined by reverse-transcription quantitative real-time PCR (RT-qPCR). In order to find conditions adequate for quantifying enteroviral RNA isolated from (waste)water we have investigated affinity capture of RNA with polystyrene beads (Dynabeads). The capture efficiency strongly depended on the genomic region chosen for the affinity binding. Capture of the RNA by its 3′-tail was most efficient (almost 100%); other regi

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Morrison, Mark, Roderick I. Mackie, and Albrecht Kistner. "3-Phenylpropanoic Acid Improves the Affinity of Ruminococcus albus for Cellulose in Continuous Culture." Applied and Environmental Microbiology 56, no. 10 (1990): 3220–22. http://dx.doi.org/10.1128/aem.56.10.3220-3222.1990.

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Rimmelzwaan, G. F., J. Groen, N. Juntti, J. S. Teppema, F. G. C. M. UytdeHaag, and A. D. M. E. Osterhaus. "Purification of infectious canine parvovirus from cell culture by affinity chromatography with monoclonal antibodies." Journal of Virological Methods 15, no. 4 (1987): 313–22. http://dx.doi.org/10.1016/0166-0934(87)90154-6.

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