Relevant bibliographies by topics / Culture cells

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Culture cells'

Author: Grafiati
Published: 4 June 2021
Last updated: 2 February 2022

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Journal articles on the topic "Culture cells"

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Skottman, Heli, and Outi Hovatta. "Culture conditions for human embryonic stem cells." Reproduction 132, no. 5 (2006): 691–98. http://dx.doi.org/10.1530/rep.1.01079.

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Human embryonic stem cell (hESC) lines have been derived and cultured in variable conditions. The idea behind derivation of hESC lines is to use them in human cell transplantation after differentiation, but already now these cells are widely used for research purposes. Despite similarities among the established lines, important differences have been reported between them, and it has been difficult to compare the results obtained using different lines. Recent optimization of hESC culture conditions has moved from cultures on mouse embryonic fibroblasts (MEFs) in fetal bovine serum-containing me
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Agius, L. "Metabolic interactions of parenchymal hepatocytes and dividing epithelial cells in co-culture." Biochemical Journal 252, no. 1 (1988): 23–28. http://dx.doi.org/10.1042/bj2520023.

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When parenchymal hepatocytes isolated from adult liver are co-cultured with other epithelial cells, the production of various plasma proteins by the hepatocytes is preserved for much longer than in conventional culture. This study examines some of the metabolic interactions between parenchymal hepatocytes and epithelial cells maintained in co-culture. The leakage of lactate dehydrogenase by hepatocytes co-cultured with epithelial cells was lower than in conventional hepatocyte culture. The epithelial cells have a high glycolytic rate and provide the hepatocytes with a continual supply of lacta
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Park, Yong H., Joshua D. Snook, Iris Zhuang, Guofu Shen, and Benjamin J. Frankfort. "Optimized culture of retinal ganglion cells and amacrine cells from adult mice." PLOS ONE 15, no. 12 (2020): e0242426. http://dx.doi.org/10.1371/journal.pone.0242426.

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Cell culture is widely utilized to study the cellular and molecular biology of different neuronal cell populations. Current techniques to study enriched neurons in vitro are primarily limited to embryonic/neonatal animals and induced pluripotent stem cells (iPSCs). Although the use of these cultures is valuable, the accessibility of purified primary adult neuronal cultures would allow for improved assessment of certain neurological diseases and pathways at the cellular level. Using a modified 7-step immunopanning technique to isolate for retinal ganglion cells (RGCs) and amacrine cells (ACs) f
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Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seekin
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Eswaramoorthy, Sindhuja D., Nandini Dhiman, Gayathri Korra, et al. "Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold." Regenerative Medicine 14, no. 7 (2019): 647–61. http://dx.doi.org/10.2217/rme-2018-0166.

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Aim: We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs co-culture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds. Methods: The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies. Results: The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression. Conclusion: These findi
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First, NL, MM Sims, SP Park, and MJ Kent-First. "Systems for production of calves from cultured bovine embryonic cells." Reproduction, Fertility and Development 6, no. 5 (1994): 553. http://dx.doi.org/10.1071/rd9940553.

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The development of totipotent bovine embryonic cell cultures has great value in cattle breeding. They provide: (1) a mechanism for making large numbers of clonal offspring by nuclear transfer; (2) an efficient gene transfer system through the use of selectable markers to select transgenic cells; and (3) a mechanism for site-specific gene transfer or deletion by homologous DNA sequence recombination. Bovine embryonic cell cultures have been established from blastocyst inner cell mass (ICM) cells, morulae and the precompaction 16-20-cell stage. All have exhibited similar morphology to mouse embr
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Engel, F. E., A. G. Khare, and B. D. Boyan. "Phenotypic Changes of Rabbit Mandibular Condylar Cartilage Cells in Culture." Journal of Dental Research 69, no. 11 (1990): 1753–58. http://dx.doi.org/10.1177/00220345900690110801.

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The present study describes the behavior of mandibular condylar cartilage (MCC) cells as a function of time in primary culture, since it is not yet clear whether these cells maintain their phenotype in culture. MCC cells from New Zealand white rabbits were seeded at high density and cultured in DMEM containing 50 μg/mL ascorbic acid and 10% fetal bovine serum. These cells appeared as a heterogeneous population and changed their shape, size, and refractivity as cultures aged. Cartilage-like cells, which always dominated the culture, were infiltrated with a minority of fibroblast-like cells. Cel
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Culp, D. J., and L. R. Latchney. "Mucinlike glycoproteins from cat tracheal gland cells in primary culture." American Journal of Physiology-Lung Cellular and Molecular Physiology 265, no. 3 (1993): L260—L269. http://dx.doi.org/10.1152/ajplung.1993.265.3.l260.

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In a recent study (D. J. Culp, D. K. P. Lee, D. P. Penney, and M. G. Marin. Am. J. Physiol. 263: L264-275, 1992), we reported that primary cultures of cat tracheal gland cells expressed histological, ultrastructural, and immunological characteristics of mucous cells when cultured on floating gels of rat tail collagen (released-gel cultures) compared with cells cultured on glutaraldehyde-fixed collagen gels (fixed-gel cultures). We therefore collected culture medium from gland cells grown under both culture conditions for determination and comparison of glycoconjugates with characteristics of m
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Huang, Xiaosong, L. Jeanne Pierce, Paul A. Cobine, Dennis R. Winge, and Gerald J. Spangrude. "Copper Modulates the Differentiation of Mouse Hematopoietic Progenitor Cells in Culture." Cell Transplantation 18, no. 8 (2009): 887–97. http://dx.doi.org/10.3727/096368909x471152.

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Copper chelation has been shown to favor the expansion of human hematopoietic stem/progenitor cells in vitro. To further understand the effects of copper modulation on defined subsets of stem cells versus progenitor cells, we extended the studies in a mouse system. We isolated mouse hematopoietic stem cells (HSCs) or hematopoietic progenitor cells (HPCs) and cultured them with or without the copper chelator tetraethylenepentamine (TEPA) or CuCl2. Cytokine-stimulated HPC cultures treated with TEPA for 7 days generated about two to three times more total and erythroid colony-forming cells (CFCs)
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Balch, Ying. "Subculture human skeletal muscle cells to produce the cells with different Culture medium compositions." Clinical Research and Clinical Trials 3, no. 4 (2021): 01–03. http://dx.doi.org/10.31579/2693-4779/036.

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This study aimed to subculture human skeletal muscle cells (HSkMC) using a culture medium with different compositions to determine the most efficient medium for the growth of the human skeletal muscle cells. The culture media was divided into three groups: Group1. An HSkMC growth medium. Group 2. An HSkMC growth medium + with 10% high glucose (GH). Group 3. An HSkMC growth medium + 10% fetal bovine serum (FBS). HSkMC from groups 1 to 3 gradually became round in shape and gathered in clusters. These changes differed between the groups. In group 3, the HSkMC clusters were more in numbers and gat
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Dissertations / Theses on the topic "Culture cells"

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Liu, Mengfei, and 刘梦菲. "Epithelial morphogenesis in three-dimensional cell culture system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208611.

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In human body, the most common structures formed by epithelial cells are hollow cysts or tubules. The key feature of the cysts and tubules is the central lumen, which is lined by epithelial cell sheets. The central lumen allows material exchange, thus it is indispensable for the proper function of the epithelial tissue. In order to understand the way that the epithelial cells form highly specialized structure, an in vitro three-dimensional (3D) culture system was established. The Caco-2 cells were embedded in reconstituted basement membrane termed matrigel, whose biochemical constitution and
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Hou, Yuen-chi Denise. "A comparative study on the effects of feeder cells on culture of human embryonic stem cells." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43703604.

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Brons, I. G. M. "Tissue culture of rat insulinoma cells." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303711.

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Hou, Yuen-chi Denise, and 侯元琪. "A comparative study on the effects of feeder cells on culture of human embryonic stem cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hdl.handle.net/10722/210317.

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Capra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.

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Abstract The epithelial cells form barriers that compartmentalize the organs. Carcinomas are cancers stemming from epithelial cells and are the most common cancer type. The aim of this study was to understand the differentiation and malignant transformation of epithelial Madin-Darby canine kidney (MDCK) cells and to analyse the electrophysiological parameters which regulate their transport capacity. Emphasis was placed on comparing different culture environments, both in 2D and 3D. First, the effects of drugs or basal extracellular fluid composition on MDCK cell, cyst and lumen volumes were an
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Reiland, Joanne Elizabeth Donovan Maureen D. "Analysis of cell culture models of mammary drug transport." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/316.

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Brodkin, Kathryn Rhea. "Chondrocyte behavior in monolayer culture : the effects of protein substrates and culture media." Thesis, Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/20216.

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Duffy, Cairnan Robert Emmett. "New culture systems for mesenchymal stem cells." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/21044.

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Mesenchymal stem cells are the stem cells that replace the bone, fat and cartilage tissues of the human body. In addition, these cells can form muscles, ligaments and neurons. This wide multipotency has made mesenchymal stem cells of particular interest in the fields of tissue engineering and regenerative medicine. Furthermore, mesenchymal stem cells can modulate the immune system by reducing factors that increase inflammation and immune recognition. This immune recognition suppression has resulted in their application as part of bone marrow transplantation in the prevention of 'graft versus h
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Kuran, Mehmet. "Studies on bovine granulosa cells in culture." Thesis, University of Aberdeen, 1995. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU543200.

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This study includes investigations into bovine granulosa cell differentiation, the response of these cells to gonadotrophin (follicle-stimulating hormone (FSH), luteinizing hormone (LH) and pregnant mares' serum gonadotrophin (PMSG)) stimulation, and the neutralizing capacity of a monoclonal antibody to PMSG (APMSG) on the response of gonadotrophin-stimulated bovine granulosa cells using a serum-free culture system. The parameters measured were cell number (mug DNA), cytoplasmic:nuclear ratio (mug protein/mug DNA), cell size (mum diameter) and cell steroidogenesis (progesterone, ng/mug DNA; oe
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Brunton, Valerie G. "Polyamine toxicity in mammalian cells in culture." Thesis, University of Aberdeen, 1990. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU546698.

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The polyamines, spermidine and spermine, and their diamine precursor putrescine, act as both positive and negative regulators of cell growth. Their oxidation to growth inhibitory aminoaldehydes by a copper-dependent amine oxidase present in ruminant serum was initially thought to be responsible for their toxicity. However, more recently, it has been shown that polyamines are toxic in the absence of ruminant serum and that oxidation by an intracellular amine oxidase may be involved. It is this area of polyamine toxicity that this work has focused on. The model used for a normal fibroblast cell
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Books on the topic "Culture cells"

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Noll, Thomas, ed. Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9.

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Drosophila cells in culture. Academic Press, 1997.

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Freshney, R. Ian. Culture of Animal Cells. John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470649367.

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Freshney, R. Ian, and Mary G. Freshney, eds. Culture of Epithelial Cells. John Wiley & Sons, Inc., 2002. http://dx.doi.org/10.1002/0471221201.

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Koller, Manfred R. Human Cell Culture: Volume IV: Primary Hematopoietic Cells. Kluwer Academic Publishers, 2002.

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Plant cell culture protocols. 3rd ed. Springer, 2012.

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Culture of animal cells: A manual of basic technique. 2nd ed. A.R. Liss, 1987.

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Culture of animal cells: A manual of basic technique. 4th ed. Wiley-Liss, 2000.

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Culture of animal cells: A manual of basic technique. 2nd ed. Wiley-Liss, 1987.

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Culture of animal cells: A manual of basic technique. 3rd ed. Wiley-Liss, 1994.

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Book chapters on the topic "Culture cells"

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Pavelka, Margit, and Jürgen Roth. "Cells in Culture." In Functional Ultrastructure. Springer Vienna, 2010. http://dx.doi.org/10.1007/978-3-211-99390-3_82.

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Pedro, Luísa, and Guilherme N. M. Ferreira. "Purification of a Chimeric Simian – Human Immunodeficiency Virus-Like Nanoparticle from HEK293 Cell Culture." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_92.

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Borgschulte, Trissa, Fanny Delegrange, Christoph L. Bausch, et al. "Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_1.

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Rose, Thomas, Anne Furthmann, Marie Etzien, Karsten Winkler, and Volker Sandig. "CHO-DG44 Cell Line Development by FLP-Targeting – High Level Glycoprotein Expression with Significantly Decreased Time Lines." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_10.

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Diederichs, Solvig, Daniel Riechers, Friederike Sempf, et al. "Investigation of the Effect of Mechanical Strain on the Osteogenic Differentiation of Mesenchymal Stem Cells." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_100.

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Röker, Stefanie, Solvig Diederichs, Victor Korzhikov, Thomas Scheper, Tatiana Tennikova, and Cornelia Kasper. "Biofunctional Polymer-Mineral Composites as Scaffolds for Bone Tissue Engineering." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_101.

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Korzhikov, V., E. Vlakh, S. Diederichs, S. Roeker, T. Tennikova, and C. Kasper. "New Water-Soluble Polymers for Construction of Biofunctionalized Scaffolds for Bone Tissue Engineering: Synthesis and Adsorption Study." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_102.

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Behr, Larissa, Pierre Moretti, Fabienne Anton, Cornelia Kasper, and Thomas Scheper. "Selection of High-Producing Cells Via Cell Sorting Using an Affinity Matrix." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_103.

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Lim, InHwan, Jung-Seob Kim, Guehwa Lee, Murim Choi, and Yeup Yoon. "The Effects of Medium Supplement on High-Level Production of Recombinant Human Factor IX in CHO Cell." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_104.

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Steinhäuser, Dirk. "Case Study Large Scale Cell Culture Facility." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_105.

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Conference papers on the topic "Culture cells"

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Isu, Giuseppe, Diana Massai, Giulia Cerino, et al. "A Novel Perfusion Bioreactor for 3D Cell Culture in Microgravity Conditions." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14502.

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Cell suspension culture methods based on the generation of microgravity environment are widely used in regenerative medicine for (1) the production of native-like three-dimensional (3D) cell aggregates and engineered tissues [1,2,3], for (2) low cost scalable cell expansion and long-term cell viability maintenance [4,5], and for (3) guiding differentiation of stem cells (SCs) [6]. The generation of a microgravity environment for 3D cell cultures, mimicking the native environment, promotes spatial freedom, cell growth, cell-cell interaction and improves mass transfer and cell exposure to nutrie
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Berthier, R., A. Duperray, O. Valiron, M. Prenant, I. Newton, and A. Schweitzer. "MEGAKARYOCYTIC DEVELOPMENT IN LIQUID CULTURES OF CRYOPRESERVED LEUKOCYTE STEM CELL CONCENTRATES FROM CHRONIC MYELOGENOUS LEUKEMIA PATIENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644622.

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The proliferation and differentiation of human megakaryocytes in liquid culture has been obtained using cryopreserved light density blood cell concentrates from chronic myelogenous leukemia (CML) patients. These cryopreserved leukocytes concentrates contain a large number of viable granulo-monocytic, erythroid and megakaryocytic committed stem cells. A high number of spontaneous megakaryocytic colonies was observed in semisolid cultures plated with the CML leukocytes concentrates. A liquid culture system using RPMI 1640 supplemented with 20% human plasma (HP) has been defined where maturing me
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Suzuki, Kei, Toshihiko Shiraishi, Shin Morishita, and Hiroshi Kanno. "Effects of Mechanical Vibration on Proliferation and Differentiation of Neural Stem Cells." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66831.

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Neural stem cells have been studied to promote neurogenesis in regenerative therapy. The control of differentiation of neural stem cells to nerve cells and the increase of the number of nerve cells are needed. For the purpose of them, it is important to investigate not only chemical factors but also mechanical factors such as hydrostatic pressure in brain and mechanical vibration in walking. In this study, sinusoidal inertia force was applied to cultured neural stem cells and the effects of mechanical vibration on the cells were investigated. After the cells were cultured in culture plates for
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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarr
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Shiraishi, Toshihiko, Kei Suzuki, Shin Morishita, and Hiroshi Kanno. "Control of Apoptosis and Differentiation of Cultured Neural Stem Cells by Mechanical Vibration." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11154.

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In this study, sinusoidal inertia force was applied to cultured neural stem cells and the effects of mechanical vibration on the cells were investigated. Neural stem cells which were obtained from the hippocampus of an adult Fischer rat were seeded in culture plates at the density of 2.5 × 105 cells/ml. After cells were cultured for one day and adhered on the cultured plate, vibration groups of the culture plates were set on the aluminum plate of the experimental setup and cultured under sinusoidal excitation in another CO2 incubator separated from non-vibration groups of the culture plates. A
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Shiraishi, Toshihiko, Kazutaka Ohashi, Shin Morishita, and Ryohei Takeuchi. "Investigation of Mechanism of Proliferation Promotion of Cultured Osteoblasts by Mechanical Vibration." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-64845.

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This paper describes effects of mechanical vibration on osteoblasts. Their cell proliferation was investigated when sinusoidal inertia force was applied to the cells. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibration group of the culture plates was set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation of 0.5 G and 12.5 Hz for 24 hours a day in another incubator separated from non-vibration group during 21 days of culture. The time evolution of cell density was obtained by counting the nu
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Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

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Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. I
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Piovella, F., R. Lombardi, M. Vigotti, A. B. Federici, P. M. Mannucci, and E. Ascari. "VON WILLEBRAND FACTOR MULTIMERS IN CULTURED HUMAN ENDOTHELIAL CELLS: COMPARISON BETWEEN CELLULAR STORAGE POOL AND SUPERNATANT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644100.

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The multimeric composition of von Willebrand factor (vWf) from cultured human endothelial cells (h.e.c.) has been compared with the multimeric composition of vWf from h.e.c. culture supernatant, human normal platelets and plasma. H.e.c. were derived from umbilical cord veins by collagenase digestion, seeded in culture flasks and grown to confluence in TC 199 culture medium, supplemented with 20% foetal calf serum (FCS). At confluency, cells were harvested by rubber policeman, resuspended in 500 |o.l HBSS with protease inhibitors and stored with culture media until assay. H.e.c. and platelet ly
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Suganuma, Lisa, Hiromichi Fujie, Hiroki Sudama, et al. "Nanostructure Processed on Culture Plate Improves Cell Adhesion." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53753.

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Ligaments and tendons have superior functions, but their healing capacities are limited. We have been developing a novel tissue-engineering technique for the repair of ligaments and tendons which involve stem cell-based self-assembled tissues (scSAT) derived from synovium[1]. For biological reconstruction of soft tissues, it is required for the scSAT to have high tensile strength. Our previous study indicted that, when the scSAT was cultured under high cell density condition, the tensile strength of the scSAT become higher than that cultured under low density condition[2]. However, the scSAT h
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Wautier, M. P., and J. L. Wautier. "HUMAN ENDOTHELIAL CELLS MODIFICATIONS DURING LONG TERM CULTURE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643347.

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The culture of human endothelial cells is largely used for vascular research. The possibility of developping long term culture of human endothelial cells (EC) raised the question regarding the identity after several passages. To further investigate this aspect we have cultured human umbilical vein EC until the 12th passage on fibronectin coated dishes supplemented with ECGF. We have studied the EC morphology by light and electron microscopy, the reactivity with 51Cr labelled platelets, and prostacyclin synthesis. Until the 6th passage no major change could be noted, except the occurence of rar
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Reports on the topic "Culture cells"

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Malik, Abir, D. Lam, H. A. Enright, S. K. G. Peters, B. Petkus, and N. O. Fischer. Characterizing the Phenotypes of Brain Cells in a 3D Hydrogel Cell Culture Model. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1466140.

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Novaro, Virginia, and Mina BIssell. A Cell Culture Model for Understanding Estrogen Receptor Regulation in Normal and Malignant Cells. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada373393.

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Merkle, Carrie J. Studies on Breast Cancer Cell Interactions with Aged Endothelial Cells in Culture and Rat Models. Defense Technical Information Center, 2006. http://dx.doi.org/10.21236/ada455981.

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Richmond, Robert C. Cell and Molecular Biology of Ataxia Telangiectasia Heterozygous Human Mammary Epithelial Cells Irradiated in Culture. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada412826.

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Chapatwala, K. D. Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Office of Scientific and Technical Information (OSTI), 1993. http://dx.doi.org/10.2172/6435746.

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Chapatwala, K. D. Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Final report: June 1992--June 1994. Office of Scientific and Technical Information (OSTI), 1994. http://dx.doi.org/10.2172/10104750.

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Felton, James S. Quantifying the Effects of Preventive Foods on the Metabolism of a Prostate Carcinogen in Humans and in Prostate Cells Grown in Culture. Defense Technical Information Center, 2004. http://dx.doi.org/10.21236/ada428020.

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Latimer, Jean J. Identification of Stem Cells in a Novel Human Mammary Epithelial Culture (HMEC) System that Reproducibly Demonstrates Ductal Organotypic Architecture in 3 Weeks. Defense Technical Information Center, 2006. http://dx.doi.org/10.21236/ada458403.

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Chapatwala, K. D. Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Annual progress report, June 1992--May 1993. Office of Scientific and Technical Information (OSTI), 1993. http://dx.doi.org/10.2172/10159409.

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Kanatous, Shane B. Proof of Concept to Isolate and Culture Primary Muscle Cells from Northern Elephant Seals to Study the Mechanisms that Maintain Aerobic Metabolism Under the Hypoxic Conditions of Breath-hold Diving. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada573541.

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