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Journal articles on the topic 'Culture cells'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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1

Skottman, Heli, and Outi Hovatta. "Culture conditions for human embryonic stem cells." Reproduction 132, no. 5 (2006): 691–98. http://dx.doi.org/10.1530/rep.1.01079.

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Human embryonic stem cell (hESC) lines have been derived and cultured in variable conditions. The idea behind derivation of hESC lines is to use them in human cell transplantation after differentiation, but already now these cells are widely used for research purposes. Despite similarities among the established lines, important differences have been reported between them, and it has been difficult to compare the results obtained using different lines. Recent optimization of hESC culture conditions has moved from cultures on mouse embryonic fibroblasts (MEFs) in fetal bovine serum-containing me

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2

Agius, L. "Metabolic interactions of parenchymal hepatocytes and dividing epithelial cells in co-culture." Biochemical Journal 252, no. 1 (1988): 23–28. http://dx.doi.org/10.1042/bj2520023.

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When parenchymal hepatocytes isolated from adult liver are co-cultured with other epithelial cells, the production of various plasma proteins by the hepatocytes is preserved for much longer than in conventional culture. This study examines some of the metabolic interactions between parenchymal hepatocytes and epithelial cells maintained in co-culture. The leakage of lactate dehydrogenase by hepatocytes co-cultured with epithelial cells was lower than in conventional hepatocyte culture. The epithelial cells have a high glycolytic rate and provide the hepatocytes with a continual supply of lacta

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3

Park, Yong H., Joshua D. Snook, Iris Zhuang, Guofu Shen, and Benjamin J. Frankfort. "Optimized culture of retinal ganglion cells and amacrine cells from adult mice." PLOS ONE 15, no. 12 (2020): e0242426. http://dx.doi.org/10.1371/journal.pone.0242426.

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Cell culture is widely utilized to study the cellular and molecular biology of different neuronal cell populations. Current techniques to study enriched neurons in vitro are primarily limited to embryonic/neonatal animals and induced pluripotent stem cells (iPSCs). Although the use of these cultures is valuable, the accessibility of purified primary adult neuronal cultures would allow for improved assessment of certain neurological diseases and pathways at the cellular level. Using a modified 7-step immunopanning technique to isolate for retinal ganglion cells (RGCs) and amacrine cells (ACs) f

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4

Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seekin

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Eswaramoorthy, Sindhuja D., Nandini Dhiman, Gayathri Korra, et al. "Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold." Regenerative Medicine 14, no. 7 (2019): 647–61. http://dx.doi.org/10.2217/rme-2018-0166.

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Aim: We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs co-culture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds. Methods: The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies. Results: The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression. Conclusion: These findi

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First, NL, MM Sims, SP Park, and MJ Kent-First. "Systems for production of calves from cultured bovine embryonic cells." Reproduction, Fertility and Development 6, no. 5 (1994): 553. http://dx.doi.org/10.1071/rd9940553.

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The development of totipotent bovine embryonic cell cultures has great value in cattle breeding. They provide: (1) a mechanism for making large numbers of clonal offspring by nuclear transfer; (2) an efficient gene transfer system through the use of selectable markers to select transgenic cells; and (3) a mechanism for site-specific gene transfer or deletion by homologous DNA sequence recombination. Bovine embryonic cell cultures have been established from blastocyst inner cell mass (ICM) cells, morulae and the precompaction 16-20-cell stage. All have exhibited similar morphology to mouse embr

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7

Engel, F. E., A. G. Khare, and B. D. Boyan. "Phenotypic Changes of Rabbit Mandibular Condylar Cartilage Cells in Culture." Journal of Dental Research 69, no. 11 (1990): 1753–58. http://dx.doi.org/10.1177/00220345900690110801.

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The present study describes the behavior of mandibular condylar cartilage (MCC) cells as a function of time in primary culture, since it is not yet clear whether these cells maintain their phenotype in culture. MCC cells from New Zealand white rabbits were seeded at high density and cultured in DMEM containing 50 μg/mL ascorbic acid and 10% fetal bovine serum. These cells appeared as a heterogeneous population and changed their shape, size, and refractivity as cultures aged. Cartilage-like cells, which always dominated the culture, were infiltrated with a minority of fibroblast-like cells. Cel

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8

Culp, D. J., and L. R. Latchney. "Mucinlike glycoproteins from cat tracheal gland cells in primary culture." American Journal of Physiology-Lung Cellular and Molecular Physiology 265, no. 3 (1993): L260—L269. http://dx.doi.org/10.1152/ajplung.1993.265.3.l260.

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In a recent study (D. J. Culp, D. K. P. Lee, D. P. Penney, and M. G. Marin. Am. J. Physiol. 263: L264-275, 1992), we reported that primary cultures of cat tracheal gland cells expressed histological, ultrastructural, and immunological characteristics of mucous cells when cultured on floating gels of rat tail collagen (released-gel cultures) compared with cells cultured on glutaraldehyde-fixed collagen gels (fixed-gel cultures). We therefore collected culture medium from gland cells grown under both culture conditions for determination and comparison of glycoconjugates with characteristics of m

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9

Huang, Xiaosong, L. Jeanne Pierce, Paul A. Cobine, Dennis R. Winge, and Gerald J. Spangrude. "Copper Modulates the Differentiation of Mouse Hematopoietic Progenitor Cells in Culture." Cell Transplantation 18, no. 8 (2009): 887–97. http://dx.doi.org/10.3727/096368909x471152.

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Copper chelation has been shown to favor the expansion of human hematopoietic stem/progenitor cells in vitro. To further understand the effects of copper modulation on defined subsets of stem cells versus progenitor cells, we extended the studies in a mouse system. We isolated mouse hematopoietic stem cells (HSCs) or hematopoietic progenitor cells (HPCs) and cultured them with or without the copper chelator tetraethylenepentamine (TEPA) or CuCl2. Cytokine-stimulated HPC cultures treated with TEPA for 7 days generated about two to three times more total and erythroid colony-forming cells (CFCs)

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10

Balch, Ying. "Subculture human skeletal muscle cells to produce the cells with different Culture medium compositions." Clinical Research and Clinical Trials 3, no. 4 (2021): 01–03. http://dx.doi.org/10.31579/2693-4779/036.

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This study aimed to subculture human skeletal muscle cells (HSkMC) using a culture medium with different compositions to determine the most efficient medium for the growth of the human skeletal muscle cells. The culture media was divided into three groups: Group1. An HSkMC growth medium. Group 2. An HSkMC growth medium + with 10% high glucose (GH). Group 3. An HSkMC growth medium + 10% fetal bovine serum (FBS). HSkMC from groups 1 to 3 gradually became round in shape and gathered in clusters. These changes differed between the groups. In group 3, the HSkMC clusters were more in numbers and gat

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11

Honda, B. D., and N. T. Glanville. "Glucose oxidation by adherent and mechanically detached cultured cells." Biochemistry and Cell Biology 69, no. 10-11 (1991): 728–30. http://dx.doi.org/10.1139/o91-109.

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This study examined the effect of mechanical detachment from the growth surface on energy metabolism of cultured cells. Oxidation of [1-14C]glucose measured by production of 14CO2 by adherent neuroblastoma (123 ± 5 nmol/mg protein per minute), glioma (128 ± 10 nmol/mg protein per minute), and fibroblast (137 ± 5 nmol/mg protein per minute) cultures was similar. Removing cells from the culture flask by scraping reduced glucose oxidation by 62, 30, and 82% in neuroblastoma, glioma, and fibroblast cultures, respectively. Transferring cells from a culture flask to a test tube, to control for diffu

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Dickman, K. G., and J. L. Renfro. "Primary culture of flounder renal tubule cells: transepithelial transport." American Journal of Physiology-Renal Physiology 251, no. 3 (1986): F424—F432. http://dx.doi.org/10.1152/ajprenal.1986.251.3.f424.

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Renal proximal tubule cells from the winter flounder (Pseudopleuronectes americanus) were maintained in a functionally differentiated state for up to 16 days in primary culture on floating collagen gels. The cells were confluent after 7-8 days in culture, contracted the collagen gels, and exhibited ciliary activity. Electron microscopy indicated that the cultures were composed of continuous sheets of columnar epithelial cells that had established structural polarity. When mounted in Ussing chambers, the cultures exhibited a small mucosa-negative potential difference (0.6 +/- 0.10 mV) and a low

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Stano, J., K. Mičieta, E. Tokhtaeva, M. Valšíková, M. Koreňová, and V. Blanáriková. "Demonstration of lactase activity in culture medium of melon cells." Horticultural Science 31, No. 4 (2011): 132–35. http://dx.doi.org/10.17221/3806-hortsci.

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Lactase activity was detected in a&nbsp;culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A&nbsp;simple, rapid and reproducible procedure for identification of extracellular lactase is described using callus cultures of seedlings from the tested plant, hairy roots of 2.5 days old seedlings of watermelon germinating on agar plates as well as cell suspension cultures derived from callus cultures. For the determination of intracellular activities of lactase, 6-bromo-2-naphthyl-&beta;-D-galactopyranoside and p-nitrophenyl-&beta;-D-galactopyranosid

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14

RemyaV, RemyaV, Naveen Kumar, and Kutty M. V. H. Kutty M.V.H. "A Method for Cell Culture and RNA Extraction of Rabbit Bone Marrow Derived Mesenchymal Stem Cells." International Journal of Scientific Research 3, no. 7 (2012): 31–33. http://dx.doi.org/10.15373/22778179/july2014/11.

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15

Culp, D. J., D. K. Lee, D. P. Penney, and M. G. Marin. "Cat tracheal gland cells in primary culture." American Journal of Physiology-Lung Cellular and Molecular Physiology 263, no. 2 (1992): L264—L275. http://dx.doi.org/10.1152/ajplung.1992.263.2.l264.

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Conditions for the primary culture of isolated cat tracheal gland cells were established. Cells plated onto glutaraldehyde-fixed gels of rat tail collagen grew to confluency after 8 days of culture forming a monolayer of cuboidal cells with ultrastructural characteristics of epithelial cells and immunoreactivity to antikeratins. Cultured cells synthesized and released radiolabeled high-molecular-weight glycoconjugates. Glycoconjugate secretion was increased approximately 10% in response to the cholinergic agonist, carbachol. Secretion of glycoconjugates was unrelated to regulated exocrine secr

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16

Bowers, W. E., and M. R. Berkowitz. "Differentiation of dendritic cells in cultures of rat bone marrow cells." Journal of Experimental Medicine 163, no. 4 (1986): 872–83. http://dx.doi.org/10.1084/jem.163.4.872.

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Although dendritic cells (DC) originate from bone marrow, they were not observed in fresh preparations of bone marrow cells (BMC). Likewise, accessory activity was barely measurable in a sensitive assay for this potent function of DC. However, both DC and accessory activity developed when BMC were cultured for 5 d. Based on fractionation before culture, nearly all of the accessory activity could be attributed to only 5% of the total BMC recovered in a low-density (LD) fraction. The LD-DC precursors differed from mature DC in a number of important respects. Removal of Ia+ cells from the LD frac

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17

Orlidge, A., and P. A. D'Amore. "Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells." Journal of Cell Biology 105, no. 3 (1987): 1455–62. http://dx.doi.org/10.1083/jcb.105.3.1455.

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Morphological studies of developing capillaries and observations of alterations in capillaries associated with pathologic neovascularization indicate that pericytes may act as suppressors of endothelial cell (EC) growth. We have developed systems that enable us to investigate this possibility in vitro. Two models were used: a co-culture system that allowed direct contact between pericytes and ECs and a co-culture system that prevented physical contact but allowed diffusion of soluble factors. For these studies, co-cultures were established between bovine capillary ECs and the following growth-

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18

Cardier, Jose E., Olga Wittig, Jose Alonso, and Egidio Romano. "Generation of Endothelial Progenitor Cells from Amniotic Fluid Stem Cells." Blood 112, no. 11 (2008): 5403. http://dx.doi.org/10.1182/blood.v112.11.5403.5403.

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Abstract Recently it has been shown that human amniotic fluid contains stem cells of fetal origin (AFS) that express embryonic and adult stem cell markers. AFS can be expanded extensively in culture and give rise to multiple differentiated cells such those of myogenic, osteogenic, neurogenic and endothelial lineages, all of them with potential therapeutic value. In this study, we investigated the generation of endothelial progenitor cells (EPC) from AFS. For this purpose, remnant amniotic fluid was obtained from mothers undergoing amniocentesis for medical reasons at 18 weeks of pregnancy. Cel

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19

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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20

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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21

Finoli, Anthony, Eva Schmelzer, Patrick Over, Ian Nettleship, and Joerg C. Gerlach. "Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/6040146.

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Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support. Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring. Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which

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Lukavský, Jaromír, and Vladislav Cepák. "Motile cells in outdoor mass culture of Scenedesmus obliquus II." Algological Studies/Archiv für Hydrobiologie, Supplement Volumes 91 (November 30, 1998): 101–8. http://dx.doi.org/10.1127/algol_stud/91/1998/101.

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Guo, W., K. Kamiya, J. Cheng, and J. Toyama. "Changes in action potentials and ion currents in long-term cultured neonatal rat ventricular cells." American Journal of Physiology-Cell Physiology 271, no. 1 (1996): C93—C102. http://dx.doi.org/10.1152/ajpcell.1996.271.1.c93.

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A primary culture of neonatal ventricular myocytes isolated from day-old rats was established for investigating the changes in action potentials and ion currents over long periods. Cells at days 5 and 15 in culture were studied. These changes in vitro were compared with those in situ derived from the age-matched freshly isolated cells. During primary culture, quiescent cells demonstrated shortening of action potential durations (APD) resembling the developmental changes observed in situ. The beating cultured cells were not associated with APD shortening. Despite constant current amplitudes, th

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Harrison, DE, CP Lerner, and E. Spooncer. "Erythropoietic repopulating ability of stem cells from long-term marrow culture." Blood 69, no. 4 (1987): 1021–25. http://dx.doi.org/10.1182/blood.v69.4.1021.1021.

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Abstract Hemopoietic precursors are heterogeneous with respect to their capacity for self-renewal and long-term repopulating ability. Bone marrow cultures produce a variety of precursors over many weeks, including CFU- S; however, it is important to determine whether these populations retain the functional ability shown by fresh marrow. The most primitive precursor or stem cells have the most long-term repopulating ability. We here describe direct measurements of this ability in cells from marrow cultures by using competitive repopulation assays. Cultured adherent cells repeatedly showed less

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Harrison, DE, CP Lerner, and E. Spooncer. "Erythropoietic repopulating ability of stem cells from long-term marrow culture." Blood 69, no. 4 (1987): 1021–25. http://dx.doi.org/10.1182/blood.v69.4.1021.bloodjournal6941021.

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Hemopoietic precursors are heterogeneous with respect to their capacity for self-renewal and long-term repopulating ability. Bone marrow cultures produce a variety of precursors over many weeks, including CFU- S; however, it is important to determine whether these populations retain the functional ability shown by fresh marrow. The most primitive precursor or stem cells have the most long-term repopulating ability. We here describe direct measurements of this ability in cells from marrow cultures by using competitive repopulation assays. Cultured adherent cells repeatedly showed less capacity

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Schuler, G., and R. M. Steinman. "Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro." Journal of Experimental Medicine 161, no. 3 (1985): 526–46. http://dx.doi.org/10.1084/jem.161.3.526.

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Murine epidermal Langerhans cells (LC) have been studied in tissue culture and compared to spleen dendritic cells (DC). LC comprised 3% of the starting cell suspensions and were distinguished from keratinocytes by cytology and reactivity with anti-Ia and anti-Mac-1 monoclonal antibodies. The LC were nonadherent, had a low buoyant density, did not proliferate, and could be enriched to 10-50% purity. LC continued to exhibit Ia and Mac-1 antigens for 4 d in culture. However, LC rapidly lost Birbeck granules, Fc receptors, F4/80 antigen, and cytochemical reactivity for nonspecific esterase and mem

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Wickramasinghe, S. N. "Book review: Culture of epithelial cells and Culture of hematopoietic cells." Cell Biochemistry and Function 14, no. 1 (1996): 76. http://dx.doi.org/10.1002/(sici)1099-0844(199603)14:1<76::aid-cbf1646>3.0.co;2-6.

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Jarrahy, Reza, Weibiao Huang, George H. Rudkin, et al. "Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds." American Journal of Physiology-Cell Physiology 289, no. 2 (2005): C408—C414. http://dx.doi.org/10.1152/ajpcell.00196.2004.

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Osteogenic differentiation of osteoprogenitor cells in three-dimensional (3D) in vitro culture remains poorly understood. Using quantitative real-time RT-PCR techniques, we examined mRNA expression of alkaline phosphatase, osteocalcin, and vascular endothelial growth factor (VEGF) in murine preosteoblastic MC3T3-E1 cells cultured for 48 h and 14 days on conventional two-dimensional (2D) poly(l-lactide-co-glycolide) (PLGA) films and 3D PLGA scaffolds. Differences in VEGF secretion and function between 2D and 3D culture systems were examined using Western blots and an in vitro Matrigel-based ang

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Blennerhassett, M. G., M. S. Kannan, and R. E. Garfield. "Density-dependent hyperpolarization in cultured aortic smooth muscle cells." American Journal of Physiology-Cell Physiology 256, no. 3 (1989): C644—C651. http://dx.doi.org/10.1152/ajpcell.1989.256.3.c644.

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The membrane potential (Em) of cultured aortic smooth muscle cells from Sprague-Dawley (SD), Wistar-Kyoto (WKY), and spontaneously hypertensive (SHR) rats was measured in proliferating primary cultures. Em of SD cells in high-density cultures was -51 to -58 mV, whereas that of low-density cultures (1-2 days) was -30 mV. This difference was due to a continuous process of hyperpolarization during proliferation in culture. Em of WKY and SHR hyperpolarized similarly, from -12 to -42 and -38 mV, respectively. Hyperpolarization of Em of SD, WKY, and SHR cells was related to cell density rather than

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Kim, Jeong A., Nam D. Tran, Shur-Jen Wang, and Mark Fisher. "Pericytes Regulate Fibrinolytic Function of Brain Capillary Endothelial Cells." Stroke 32, suppl_1 (2001): 359. http://dx.doi.org/10.1161/str.32.suppl_1.359-a.

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P110 Our prior work has shown that astrocytes inhibit fibrinolysis of brain capillary endothelial cells (eg, Stroke 1999:30;1671–1677). The complex cellular microenvironment at the blood-brain barrier includes pericytes, which are adjacent to and share basement membrane with brain capillary endothelial cells. We analyzed the hemostatic regulatory role of pericytes in a blood-brain barrier model consisting of human brain pericytes cultured on transwell membrane inserts with human brain capillary endothelial cells. We measured fibrinolysis proteins and function in media conditioned by 48 hour co

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Verfaillie, CM, and JS Miller. "CD34+/CD33- cells reselected from macrophage inflammatory protein 1 alpha+interleukin-3--supplemented "stroma-noncontact" cultures are highly enriched for long-term bone marrow culture initiating cells." Blood 84, no. 5 (1994): 1442–49. http://dx.doi.org/10.1182/blood.v84.5.1442.1442.

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Abstract Human hematopoietic stem cells are thought to express the CD34 stem cell antigen, low numbers of HLA-DR and Thy1 antigens, but no lineage commitment antigens, CD38, or CD45RA antigens. However, fluorescence- activated cell sorted CD34+ subpopulations contain not more than 1% to 5% primitive progenitors capable of initiating and sustaining growth in long-term bone marrow culture initiating cells (LTBMC-ICs). We have recently shown that culture of fresh human marrow CD34+/HLA-DR- cells separated from a stromal layer by a microporous membrane (“stroma- noncontact” culture) results in the

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Verfaillie, CM, and JS Miller. "CD34+/CD33- cells reselected from macrophage inflammatory protein 1 alpha+interleukin-3--supplemented "stroma-noncontact" cultures are highly enriched for long-term bone marrow culture initiating cells." Blood 84, no. 5 (1994): 1442–49. http://dx.doi.org/10.1182/blood.v84.5.1442.bloodjournal8451442.

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Human hematopoietic stem cells are thought to express the CD34 stem cell antigen, low numbers of HLA-DR and Thy1 antigens, but no lineage commitment antigens, CD38, or CD45RA antigens. However, fluorescence- activated cell sorted CD34+ subpopulations contain not more than 1% to 5% primitive progenitors capable of initiating and sustaining growth in long-term bone marrow culture initiating cells (LTBMC-ICs). We have recently shown that culture of fresh human marrow CD34+/HLA-DR- cells separated from a stromal layer by a microporous membrane (“stroma- noncontact” culture) results in the maintena

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Chute, John P., Garrett G. Muramoto, Jennifer Fung, and Carol Oxford. "Soluble factors elaborated by human brain endothelial cells induce the concomitant expansion of purified human BM CD34+CD38– cells and SCID-repopulating cells." Blood 105, no. 2 (2005): 576–83. http://dx.doi.org/10.1182/blood-2004-04-1467.

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AbstractThe CD34+CD38– phenotype identifies a population in the bone marrow that is enriched in the steady state for hematopoietic stem cells (HSCs). Following ex vivo culture of CD34+ cells, HSC content is difficult to measure since committed CD34+CD38+ progenitors down-regulate CD38 surface expression during culture. In this study, we sought to define the phenotype of human HSCs following ex vivo culture under conditions that support the expansion of human cells capable of repopulating non-obese diabetic/severe combined immunodeficiency (SCID)–repopulating cells (SRCs). Contact coculture of

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Liedtke, C. M. "Differentiated properties of rabbit tracheal epithelial cells in primary culture." American Journal of Physiology-Cell Physiology 255, no. 6 (1988): C760—C770. http://dx.doi.org/10.1152/ajpcell.1988.255.6.c760.

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The purpose of this study was to establish whether rabbit tracheal epithelial cells, grown in primary cell culture, retain neurohormonal receptor and mediator activity. Epithelial cells were isolated by enzymatic treatment and cultured on a collagen matrix. The culture medium consisted of a 1:1 mixture of Dulbecco's modified Eagle medium and Ham's F12 supplemented with 5% fetal calf serum, epidermal growth factor, insulin, transferrin, fibronectin, hydrocortisone, and trace elements. Cultures had a population doubling time of 48 h. Mucus-containing cells and cilia were not observed after 7 day

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Smith, Stephen. "Cultured CD41a+ Megakaryocytes Derived from Cord Blood CD34+ Cells Are Activated Via STAT5 but Not STAT3." Blood 106, no. 11 (2005): 4240. http://dx.doi.org/10.1182/blood.v106.11.4240.4240.

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Abstract Lengthy periods of thrombocytopenia and prolonged platelet recovery (typically, a side-affect of high-dose chemotherapy and/or radiation) have been associated with cord blood transplants. It maybe possible to alleviate the period of thrombocytopenia using cultured megakaryocytes derived from CD34+ stem cells however, platelet development invitro is delayed. Using immuno-magnetic beads, CD34+ stem cells were isolated from Cord Blood (CB) units and then cultured in serum-free culture medium (X-VIVO-10®, BioWhittaker Co. Walkersville, MD) supplemented with Thrombopoietin Peptide Agonist

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Masala, Erico, Francesca Buchi, Serena Pillozzi, et al. "Hypoxia Increases Repopulating Ability of Myelodysplastic Syndrome Bone Marrow Cells." Blood 126, no. 23 (2015): 4753. http://dx.doi.org/10.1182/blood.v126.23.4753.4753.

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Abstract Introduction: Myelodysplastic Syndromes (MDS) are clonal neoplasms. Whether the transforming event occurs in a myeloid committed cell or in earlier hematopoietic progenitor/stem cell it is still not ascertained. We evaluated the repopulating ability and stem cell potential of hypoxia maintained primary bone marrow (BM) progenitors derived from Myelodysplastic Syndromes (MDS) patients, and their capacity of engraftment in sublethally irradiated NOD-SCID mice. Methods: Thirty eight BM samples were obtained at diagnosis from MDS patients (WHO: 9 RA, 12 RCMD, 7 RAEB, 8 AML/post MDS, 2 Del

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Phillips, Tania, and Olaniyi Kehinde. "EPIDERMAL CELLS IN CULTURE." Lancet 330, no. 8565 (1987): 976. http://dx.doi.org/10.1016/s0140-6736(87)91466-8.

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38

Saunders, S. "Drosophila Cells in Culture." Cell Biology International 23, no. 9 (1999): 651. http://dx.doi.org/10.1006/cbir.1999.0446.

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ROLLWAGEN, FLORENCE M., and OSIAS STUTMAN. "CULTURE-GENERATED SUPPRESSOR CELLS." Transplantation 39, no. 5 (1985): 554. http://dx.doi.org/10.1097/00007890-198505000-00019.

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40

Schumacher, Udo. "Culture of epithelial cells." Trends in Biochemical Sciences 17, no. 12 (1992): 511. http://dx.doi.org/10.1016/0968-0004(92)90343-8.

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Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.958.

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Abstract Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and l

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42

Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.bloodjournal863958.

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Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and long-term

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43

Clark, W. A., S. J. Rudnick, J. J. LaPres, M. Lesch, and R. S. Decker. "Hypertrophy of isolated adult feline heart cells following beta-adrenergic-induced beating." American Journal of Physiology-Cell Physiology 261, no. 3 (1991): C530—C542. http://dx.doi.org/10.1152/ajpcell.1991.261.3.c530.

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Catecholamine-induced beating and myocardial hypertrophy were evaluated in isolated adult feline cardiomyocytes maintained in culture for up to 30 days. Adult feline cardiomyocytes were used in this study because they displayed several unique characteristics that facilitated assessment of factors regulating cardiomyocyte hypertrophy in vitro. These characteristics included the following. 1) A single heart provides a high yield of 20-40 x 10(6) calcium-tolerant rod-shaped myocytes. 2) In culture, isolated adult feline cardiomyocytes maintain a stable population of differentiated myocytes that c

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Lacasse, J., C. Kreft, J. Gutkowska, J. Genest, and M. Cantin. "Cultured juxtaglomerular cells: production and localization of renin." Canadian Journal of Physiology and Pharmacology 65, no. 7 (1987): 1409–15. http://dx.doi.org/10.1139/y87-221.

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Trypsin- or collagenase-dispersed renal cortical cells from newborn mice were filtered and cultured. The cultures comprised 25% juxtaglomerular cells as identified by immunocytochemistry. Renin was measured by radioimmunoassay in the culture medium and in the cells at various time intervals. This in vitro system was responsive to isoproterenol, which stimulated renin release in a dose-dependent manner.

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Dasch, J. R., and P. P. Jones. "Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes." Journal of Experimental Medicine 163, no. 4 (1986): 938–51. http://dx.doi.org/10.1084/jem.163.4.938.

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Long-term cultured bone marrow cells were characterized with respect to a number of B and pre-B cell markers. Cells expressing ThB, B-220, and IgM were found within cultures set up according to the procedure of Whitlock and Witte. This culture system was modified by placing sorted pre-B cells (ThB+, IgM-) from bone marrow in culture with previously-established bone marrow adherent layers. These cultures commenced growth without the lag associated with the Whitlock cultures. These cultured nonadherent cells show a high frequency of IgM+ cells, but do not express either IgD or Ia, and we refer t

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Smith-Thomas, L. C., and J. W. Fawcett. "Expression of Schwann cell markers by mammalian neural crest cells in vitro." Development 105, no. 2 (1989): 251–62. http://dx.doi.org/10.1242/dev.105.2.251.

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During embryonic development, neural crest cells differentiate into a wide variety of cell types including Schwann cells of the peripheral nervous system. In order to establish when neural crest cells first start to express a Schwann cell phenotype immunocytochemical techniques were used to examine rat premigratory neural crest cell cultures for the presence of Schwann cell markers. Cultures were fixed for immunocytochemistry after culture periods ranging from 1 to 24 days. Neural crest cells were identified by their morphology and any neural tube cells remaining in the cultures were identifie

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Tanini, Annalisa, Maria Luisa Brandi, Umberto Modigliani, Carlo M. Rotella, and Roberto Toccafondi. "Transient lack of response to TSH of human cultured thyroid cells obtained from hyperfunctioning tissue." Acta Endocrinologica 113, no. 3 (1986): 346–54. http://dx.doi.org/10.1530/acta.0.1130346.

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Abstract. TSH-induced cAMP accumulation in cells obtained from normal and pathological thyroid tissue was studied during the first 12 days of primary culture. In normal thyroid tissue cultures (N = 7), the response of cAMP to TSH was present from the second day of culture and reached its maximum after 8 days. A similar behaviour was observed in cultures obtained from euthyroid sporadic goitres (N = 8), even if the rate of response was slightly lower than that of normal tissue. Similarly, cultured cells from euthyroid 'autonomous' nodules (N = 8) appeared to be responsive to TSH during the peri

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Miyamoto, Yoshitaka, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, and Shuji Hayashi. "Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an in vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture." Cell Medicine 9, no. 1-2 (2017): 35–44. http://dx.doi.org/10.3727/215517916x693096.

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The application of stem cells for cell therapy has been extensively studied in recent years. Among the various types of stem cells, human adipose tissue-derived stem cells (ASCs) can be obtained in large quantities with relatively few passages, and they possess a stable quality. ASCs can differentiate into a number of cell types, such as adipose cells and ectodermal cells. We therefore focused on the in vitro microenvironment required for such differentiation and attempted to induce the differentiation of human stem cells into microtissues using a microelectromechanical system. We first evalua

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Racusen, L. C., B. A. Fivush, H. Andersson, and W. A. Gahl. "Culture of renal tubular cells from the urine of patients with nephropathic cystinosis." Journal of the American Society of Nephrology 1, no. 8 (1991): 1028–33. http://dx.doi.org/10.1681/asn.v181028.

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Nephropathic cystinosis represents a prototype for lysosomal storage diseases and is the most common cause of renal tubular Fanconi's syndrome. Mechanisms of the tubular transport defects in this disease have not been defined, however, in part because the cells readily cultured from affected patients, leukocytes and fibroblasts, do not express epithelial transport functions. Except for a single autopsy report, renal tubular cells from these patients have not been studied in vitro. In these studies, noninvasive harvesting and culture of renal tubular cells from the urine of patients with cystin

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HASHIMOTO, Kenji, and Yoshihito SHIRAI. "Cultured mammalian cells for production of bioactive macromolecules. Culture engineering of aminal cells." Journal of the agricultural chemical society of Japan 63, no. 2 (1989): 204–6. http://dx.doi.org/10.1271/nogeikagaku1924.63.204.

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