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1

Brault, Jean-Baptiste, Catherine Thouvenot, Magda Cannata Serio, et al. "A polarized cell system amenable to subcellular resolution imaging of influenza virus infection." PLOS ONE 19, no. 1 (2024): e0292977. http://dx.doi.org/10.1371/journal.pone.0292977.

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The life cycle of influenza A viruses (IAV), and notably intracellular trafficking of the viral genome, depends on multiple interactions with the cellular cytoskeleton and endomembrane system. A limitation of the conventional cellular models used for mechanistic study and subcellular imaging of IAV infection is that they are cultured in two dimensions (2D) under non-polarizing conditions, and therefore they do not recapitulate the intracellular organization of the polarized respiratory epithelial cells naturally targeted by IAVs. To overcome this limitation, we developed an IAV-infection assay
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2

Hu, Wansheng, Ming Cao, Lingni Liao, et al. "An Automated Digital Microfluidic System Based on Inkjet Printing." Micromachines 15, no. 11 (2024): 1285. http://dx.doi.org/10.3390/mi15111285.

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Cellular interactions, such as intercellular communication and signal transduction, can be enhanced within three-dimensional cell spheroids, contributing significantly to cellular viability and proliferation. This is crucial for advancements in cancer research, drug testing, and personalized medicine. The dimensions of the cell spheroids play a pivotal role in their functionality, affecting cell proliferation and differentiation, intercellular interactions, gene expression, protein synthesis, drug penetration, and metabolism. Consequently, different spheroid sizes may be required for various d
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McGhee, Eric, Juan Uruena, Padraic Levings, et al. "TMOD-07. IN SITU MICROSCOPY OF DRUG AND IMMUNE CELL INTERACTIONS AGAINST BIOFABRICATED TUMOR MODELS." Neuro-Oncology 21, Supplement_6 (2019): vi263—vi264. http://dx.doi.org/10.1093/neuonc/noz175.1106.

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Abstract BACKGROUND & SIGNIFICANCE Cancer is a disease in 3-dimensions and there is a desperate need for infrastructure and models to study immuno-oncology treatment strategies in 3D. A new culture system for long-duration maturation of patient derived microtumors has been developed. This system enables in situconfocal imaging and movies of T-cells and tumor model interactions, including measurements of T-cell migration, infiltration, and killing. HYPOTHESIS: Biofabrication of 3D microtumors using bioprinting in transparent soft granular gel media can facilitate the precise arrangement and
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Jubelin, Camille, Javier Munoz-Garcia, Denis Cochonneau, Emilie Moranton, Marie-Françoise Heymann, and Dominique Heymann. "Caractérisation de la dormance d’une lignée cellulaire d’ostéosarcome en culture trois-dimensions." Morphologie 106, no. 354 (2022): S25—S26. http://dx.doi.org/10.1016/j.morpho.2022.06.025.

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5

Green, Matthew P., and Bo Hou. "Cellular Changes of Stem Cells in 3-Dimensional Culture." Journal of Oral and Maxillofacial Surgery 75, no. 11 (2017): 2477.e1–2477.e9. http://dx.doi.org/10.1016/j.joms.2017.06.007.

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6

Dey, Nandini, Yuliang Sun, Amy K. Krie, et al. "Three dimensional organotypic ex vivo culture of tissues from post-operated tumor samples: Strengths and limitations." Journal of Clinical Oncology 35, no. 15_suppl (2017): e23151-e23151. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e23151.

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e23151 Background: Evolution of tumor occurs in two phases, pretreatment phase, and posttreatment phase.Tumorigenic history and path of tumorigenic evolution determine the response of tumor cells to antitumor drug and thus the outcome of treatment. Carcinomas from the same organ-site with similar genomic alterations in different patients may vary in their tumorigenic history and their diverse paths of tumorigenic evolution which cause them respond differently to the same antitumor drugs. Uniqueness of tumorigenic history and paths of tumorigenic evolution in individual patient makes it ideal t
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Wang, Zichun, Di Zhan, Qikuan Chen, et al. "Abstract 5499: A comparison of spheroid formation using AcroCyte‘s 3-dimensional R3CE spheroid culture platform versus traditional 3D culture methods." Cancer Research 85, no. 8_Supplement_1 (2025): 5499. https://doi.org/10.1158/1538-7445.am2025-5499.

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Abstract Three-dimensional (3D) spheroid culture systems have gained attention due to their evident advantages in mimicking the in vivo tumor microenvironment, making them highly valuable for drug testing and cancer research, as the behavior of 3D-cultured cells is more reflective of in vivo cellular responses. However, traditional spheroid methods, including ultra-low attachment plates, Matrigel, and soft agar, have limitations, especially for certain cell lines that fail to form stable spheroids. Here, we introduce a novel 3D spheroid culture method using the AcroCyte R3CE plate, and compare
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8

Suderman, Michael T., Kevin B. Temeyer, Kristie G. Schlechte, and Adalberto A. Pérez de León. "Three-Dimensional Culture of Rhipicephalus (Boophilus) microplus BmVIII-SCC Cells on Multiple Synthetic Scaffold Systems and in Rotating Bioreactors." Insects 12, no. 8 (2021): 747. http://dx.doi.org/10.3390/insects12080747.

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Tick cell culture facilitates research on the biology of ticks and their role as vectors of pathogens that affect humans, domestic animals, and wildlife. Because two-dimensional cell culture doesn’t promote the development of multicellular tissue-like composites, we hypothesized that culturing tick cells in a three-dimensional (3-D) configuration would form spheroids or tissue-like organoids. In this study, the cell line BmVIII-SCC obtained from the cattle fever tick, Rhipicephalus (Boophilus) microplus (Canestrini, 1888), was cultured in different synthetic scaffold systems. Growth of the tic
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9

Clayton, Natasha P., Alanna Burwell, Heather Jensen, et al. "Preparation of Three-dimensional (3-D) Human Liver (HepaRG) Cultures for Histochemical and Immunohistochemical Staining and Light Microscopic Evaluation." Toxicologic Pathology 46, no. 6 (2018): 653–59. http://dx.doi.org/10.1177/0192623318789069.

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The use of three-dimensional (3-D) in vitro culture systems (spheroids, organoids) in biomolecular and drug discovery research has become increasingly popular. The popularity is due, in part, to a diminished reliance on animal bioassays and a desire to develop physiologically relevant cell culture systems that simulate the in vivo tissue microenvironment. Most evaluations of 3-D cultures are by confocal microscopy and high-content imaging; however, these technologies do not allow for detailed cellular morphologic assessments or permit basic hematoxylin and eosin histologic evaluations. There a
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10

Baudino, Troy A., Alex McFadden, Charity Fix, Joshua Hastings, Robert Price, and Thomas K. Borg. "Cell Patterning: Interaction of Cardiac Myocytes and Fibroblasts in Three-Dimensional Culture." Microscopy and Microanalysis 14, no. 2 (2008): 117–25. http://dx.doi.org/10.1017/s1431927608080021.

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Patterning of cells is critical to the formation and function of the normal organ, and it appears to be dependent upon internal and external signals. Additionally, the formation of most tissues requires the interaction of several cell types. Indeed, both extracellular matrix (ECM) components and cellular components are necessary for three-dimensional (3-D) tissue formationin vitro. Using 3-D cultures we demonstrate that ECM arranged in an aligned fashion is necessary for the rod-shaped phenotype of the myocyte, and once this pattern is established, the myocytes were responsible for the alignme
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11

Mortera-Blanco, Teresa, Athanasios Mantalaris, Alexander Bismarck, and Nicki Panoskaltsis. "Long-Term in Vitro Cytokine-Free and Serum-Free Culture of Human Cord Blood Mononuclear Cells in a Three-Dimensional Scaffold." Blood 114, no. 22 (2009): 503. http://dx.doi.org/10.1182/blood.v114.22.503.503.

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Abstract Abstract 503 The ability to expand cord blood (CB) cells ex vivo overcomes an important limitation to its wider clinical application in cellular therapies. The current practice of hematopoietic cell culture is based on two-dimensional (2D) tissue culture flasks or well plates which require either co-culture with allogeneic or xenogeneic stromal cells and the exogenous provision of several cytokines. This 2D culture environment is artificial and lacks the 3D cellular niches that characterise the in vivo hematopoietic inductive microenvironment. Specifically, the cultured cells are expo
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12

Oh, Ji Min, Prakash Gangadaran, Ramya Lakshmi Rajendran, Chae Moon Hong, Jaetae Lee, and Byeong-Cheol Ahn. "Different Expression of Thyroid-Specific Proteins in Thyroid Cancer Cells between 2-Dimensional (2D) and 3-Dimensional (3D) Culture Environment." Cells 11, no. 22 (2022): 3559. http://dx.doi.org/10.3390/cells11223559.

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The two-dimensional (2D) monolayer culture as a conventional method has been widely applied in molecular biology fields, but it has limited capability to recapitulate real cell environments, being prone to misinterpretation with poor prediction of in vivo behavior. Recently, the three-dimensional (3D) spheroid culture has been studied extensively. Spheroids are self-assembled cell aggregates that have biomimicry capabilities. The behavior of thyroid cancer under the 3D spheroid culture environment has been studied; however, there are no reports regarding differences in the degree of thyroid ca
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13

Furubayashi, Tomoyuki, Daisuke Inoue, Noriko Nishiyama, et al. "Comparison of Various Cell Lines and Three-Dimensional Mucociliary Tissue Model Systems to Estimate Drug Permeability Using an In Vitro Transport Study to Predict Nasal Drug Absorption in Rats." Pharmaceutics 12, no. 1 (2020): 79. http://dx.doi.org/10.3390/pharmaceutics12010079.

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Recently, various types of cultured cells have been used to research the mechanisms of transport and metabolism of drugs. Although many studies using cultured cell systems have been published, a comparison of different cultured cell systems has never been reported. In this study, Caco-2, Calu-3, Madin–Darby canine kidney (MDCK), EpiAirway and MucilAir were used as popular in vitro cell culture systems, and the permeability of model compounds across these cell systems was evaluated to compare barrier characteristics and to clarify their usefulness as an estimation system for nasal drug absorpti
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14

Poll, B., P. Buttler, H. G. GräBer, F. Lampert, and C. Becker. "Engrafting Periodontal Fibroblasts with New 3-Dimensional Polylactide Foams." International Journal of Artificial Organs 28, no. 8 (2005): 827–33. http://dx.doi.org/10.1177/039139880502800808.

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In clinical periodontics, “tissue engineering” techniques have been developed to guide the regenerative process following periodontal disease. We explored a new shaped biomaterial in order to promote cellular adhesion, proliferation and migration of periodontal cells. Granules of poly-D,L-lactide were foamed in specially designed moulds by a controlled gas-loading process. Explant cultures of periodontal tissue were seeded at different densities on the 3-dimensional scaffolds following analysis of cytotoxicity, cell proliferation and differentiation. The moulding procedure led to porous struct
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Ryu, Ji-O., Eunyoung Lee, Sang-Yun Lee та ін. "<i>In vitro</i> study on the comparison of hypoxia-inducible factor-1α expression after particulate matter exposure in 2-dimensional and 3-dimensional cell culture models". Organoid 5 (25 лютого 2025): e2. https://doi.org/10.51335/organoid.2025.5.e2.

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Background: Particulate matter (PM) can penetrate deep into the lungs, leading to diseases through the induction of hypoxia and inflammation. The hypoxia-inducible factor-1α (HIF-1α) protein is well-established as a key factor in the hypoxic response, and PM exposure has been linked to hypoxia induction. To develop a model suitable for studying PM-induced hypoxia, we compared the expression of HIF-1α protein between a conventional 2-dimensional (2D) cell culture model and a 3-dimensional (3D) culture model.Methods: BEAS-2B cells were cultured in 2D (using a 96-well plate) and 3D (utilizing a U
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16

Rende, Maria, Teresa Mortera-Blanco, Nayef Aqel, et al. "Ex Vivo Three-Dimensional (3D) Functional biomimicry for the Long-Term Cytokine-Free Culture of Human Primary Acute Myeloid Leukemia (AML)." Blood 120, no. 21 (2012): 4896. http://dx.doi.org/10.1182/blood.v120.21.4896.4896.

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Abstract Abstract 4896 Current in vitro models for the study of AML rely on cell lines or, if primary cells are used, require abnormally high concentrations of exogenous cytokines and/or stromal cells in a co-culture system. These conditions introduce bias and artifact by selecting for culture-responsive cells and do not account for the 3D leukemic growth observed in the bone marrow. We have previously shown that human AML cell lines can be cultivated in 3D synthetic polymeric scaffolds coated with collagen and fibronectin for 8 weeks. We have also shown that human cord blood mononuclear cells
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17

Healy, Mae W., Shelley N. Dolitsky, Maria Villancio-Wolter, et al. "Creating an Artificial 3-Dimensional Ovarian Follicle Culture System Using a Microfluidic System." Micromachines 12, no. 3 (2021): 261. http://dx.doi.org/10.3390/mi12030261.

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We hypothesized that the creation of a 3-dimensional ovarian follicle, with embedded granulosa and theca cells, would better mimic the environment necessary to support early oocytes, both structurally and hormonally. Using a microfluidic system with controlled flow rates, 3-dimensional two-layer (core and shell) capsules were created. The core consists of murine granulosa cells in 0.8 mg/mL collagen + 0.05% alginate, while the shell is composed of murine theca cells suspended in 2% alginate. Somatic cell viability tests and hormonal assessments (estradiol, progesterone, and androstenedione) we
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18

Pedrotty, Dawn M., Jennifer Koh, Bryce H. Davis, Doris A. Taylor, Patrick Wolf, and Laura E. Niklason. "Engineering skeletal myoblasts: roles of three-dimensional culture and electrical stimulation." American Journal of Physiology-Heart and Circulatory Physiology 288, no. 4 (2005): H1620—H1626. http://dx.doi.org/10.1152/ajpheart.00610.2003.

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Immature skeletal muscle cells, or myoblasts, have been used in cellular cardiomyoplasty in attempts to regenerate cardiac muscle tissue by injection of cells into damaged myocardium. In some studies, muscle tissue within myoblast implant sites may be morphologically similar to cardiac muscle. We hypothesized that identifiable aspects of the cardiac milieu may contribute to growth and development of implanted myoblasts in vivo. To test this hypothesis, we designed a novel in vitro system to mimic some aspects of the electrical and biochemical environment of native myocardium. This system enabl
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19

Garza, Ricardo, and Alberto Lopez. "Measuring Innovation Culture: A Synthesis of the Innovation Culture Construct and Identification of its Research Clusters." Multidisciplinary Business Review 13, no. 1 (2020): 42–55. http://dx.doi.org/10.35692/07183992.13.1.5.

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We provide an integrative review of the previous research that has measured the innovation culture construct. Our data reveal that the construct has been composed of 28 different dimensions in previous research. Furthermore, these dimensions can be graphed along two axes: presence of the dimension in the literature and importance of the dimension within every single research project in which it appears. This analysis shows that the dimensions can be integrated into a four-quadrant matrix: 1) core dimensions: those high in both importance and presence; 2) niche dimensions: those high in importa
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Musa, Muhafiza, and Ahmad Shahrul Nizam Isha@Isa. "Voluntary Policy Interventions: Elevating Safety and Health Compliance in Aircraft Ground Handling Operations." Global Journal of Engineering and Technology Review Vol.4 (4) October-December. 2019 4, no. 4 (2019): 93–100. http://dx.doi.org/10.35609/gjetr.2019.4.4(3).

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Objective – The present study intends to examine the safety culture and safety performance outcomes relationship, present the findings on safety culture dimensions and discuss in detail on the moderating role of occupational health and safety management system (OHSMS) policy interventions. Methodology/Technique – A literature research was employed to review occupational safety and health area on specific safety culture dimensions with regard to aviation (between the years 1997 to 2018) as well as law and science policy on human behavior. Findings – The findings on safety culture dimensions wer
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Straub, Timothy M., Rachel A. Bartholomew, Catherine O. Valdez, et al. "Human norovirus infection of Caco-2 cells grown as a three-dimensional tissue structure." Journal of Water and Health 9, no. 2 (2010): 225–40. http://dx.doi.org/10.2166/wh.2010.106.

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Human norovirus (hNoV) infectivity was studied using a three-dimensional model of large intestinal epithelium. Large intestine Caco-2 cells were grown in rotating wall vessel bioreactors for 18–21 days at 37°C and then transferred to 24-well tissue culture plates where they were infected with GI.1 and GII.4 human noroviruses collected from human challenge trials and various outbreak settings, respectively. Compared with uninfected cells, transmission micrographs of norovirus-infected cells displayed evidence of shortening or total loss of apical microvilli, and vacuolization. Quantitative reve
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Tostivint, Victor, Claire Racaud-Sultan, Mathieu Roumiguié, Michel Soulié, Xavier Gamé, and Jean-baptiste Beauval. "Progrès dans l’étude du cancer de la prostate : la culture cellulaire en trois dimensions reproduit ex vivo les caractéristiques des tumeurs prostatiques." La Presse Médicale 46, no. 10 (2017): 954–65. http://dx.doi.org/10.1016/j.lpm.2017.06.014.

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23

Chang, T., G. I. Bondarenko, M. Durning, K. Vielhuber, M. A. Garthwaite, and T. G. Golos. "124 A THREE-DIMENSIONAL IN VITRO IMPLANTATION MODEL WITH NONHUMAN PRIMATE EMBRYOS AND EXTRACELLULAR MATRIX UNDER VARIOUS CULTURE CONDITIONS." Reproduction, Fertility and Development 20, no. 1 (2008): 142. http://dx.doi.org/10.1071/rdv20n1ab124.

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The need for blastocyst culture and post-implantation embryo research has emerged in the past few years. Our objective is to evaluate a novel in vitro model to study implantation and placenta formation in vitro with rhesus macaque embryos under various culture conditions. A novel nonhuman primate in vitro 3-D system can provide cues for implantation and interaction with the extracellular environment not available in 2-D planar models. Optimization of such a model can be tested with diverse culture environments. We developed and evaluated an in vitro 3-D implantation model utilizing IVF-derived
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Moore, Zena T. "Teaching and testing culture: Old questions, new dimensions." International Journal of Educational Research 23, no. 7 (1995): 595–606. http://dx.doi.org/10.1016/0883-0355(96)80439-3.

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25

FRYZ, Petro. "ETHNO-CULTURAL DIMENSIONS OF THE LEMKI CHOREOGRAPHIC CULTURE." Humanities science current issues 3, no. 65 (2023): 67–72. http://dx.doi.org/10.24919/2308-4863/65-3-10.

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26

Fauzi, Iliana, Nicki Panoskaltsis, and Athanasios Mantalaris. "A Novel, Three Dimensional (3D) Culture System for the Efficient, Single-Step Erythropoietic Differentiation of Mouse Embryonic Stem Cells (mESCs)." Blood 116, no. 21 (2010): 2044. http://dx.doi.org/10.1182/blood.v116.21.2044.2044.

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Abstract Abstract 2044 Current established protocols for the culture and differentiation of embryonic stem cells (ESCs) utilise two-dimensional (2D) tissue culture flasks/dishes. These culture methods are cumbersome and inefficient involving three stages: a) maintenance/expansion of undifferentiated ESCs, b) spontaneous differentiation through formation of embryoid bodies (EBs), and c) dissociation of EBs and replating leading to the terminal differentiation to the desired lineages. One of the major challenges in the use of ESCs for the production of red blood cells is controlling their differ
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Mikušová, Marie, Petra Rydvalová, Naděžda Klabusayová, and František Konečný. "Has COVID-19 affected the organisational culture of non-governmental organisations?" E+M Ekonomie a Management 26, no. 3 (2023): 70–91. http://dx.doi.org/10.15240/tul/001/2023-3-005.

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Organisational culture, as one of the key features of any organisation, is related to its performance. This is also true for non-governmental organisations. The purpose of this research is to identify changes in the dimensions of the organisational culture of these organisations caused by the pandemic. Data from 586 respondents, identified through an online OCAI questionnaire, were collected for pre-COVID-19, current, and preferred state. Statistically significant representation of the dimensions was identified in the types of culture. It was found that hierarchy culture was prevalent in pre-C
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Gillani, Yasir, and Nasreen Akhtar. "Safety Culture and Its Underlying Dimensions: Emergency Services Personal Perspective." Qlantic Journal of Social Sciences and Humanities 6, no. 2 (2025): 93–102. https://doi.org/10.55737/qjssh.vi-ii.25354.

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Poor workplace safety is causing approximately 2.78 million casualties daily, which leads to a considerable burden of workplace injuries/ diseases costing approximately 4% of the annual GDP of the world (ILO, 2020). To explore the perspective and underlying dimensions of the safety culture of Emergency Services Personnel (Rescue 1122), the present investigation was conducted from 10-5-2023 to 9-5-2024 in the city of Lahore, Punjab. A purposive sample of 12 Participants (9 men and 3 women, including 3 managers, 3 trainers, 3 middle managers, and 3 participants) who had been serving the departme
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Gutschmidt, Daniela, and Antonio Vera. "Dimensions of police culture – a quantitative analysis." Policing: An International Journal 43, no. 6 (2020): 963–77. http://dx.doi.org/10.1108/pijpsm-06-2020-0089.

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PurposeMany authors describe police culture as a relevant determinant of officers' health, policing behavior and reaction to change. Investigation of such relationships requires an appropriate instrument for measuring police culture.Design/methodology/approachThis paper proposes a questionnaire containing 20 values that are characteristic of police culture (e.g. masculinity, loyalty, solidarity). In an online survey, 153 German police officers described their last workgroups in terms of how typical these values are. Besides conducting item and factor analyses, multiple regression models were t
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Cook, James L., John M. Kreeger, John T. Payne, and James L. Tomlinson. "Three-dimensional culture of canine articular chondrocytes on multiple transplantable substrates." American Journal of Veterinary Research 58, no. 4 (1997): 419–24. http://dx.doi.org/10.2460/ajvr.1997.58.04.419.

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Abstract Objective To determine the effects of transplantable substrates on canine chondrocytes grown in three-dimensional culture. Animals 3 canine cadavers. Procedure Articular cartilage harvested from canine cadavers was used to obtain chondrocytes for primary culture. Subcultured chondrocytes were grown in agarose alone (AG), or in agarose on canine cancellous bone (CB), polypropylene mesh, or oxidized regenerated cellulose substrate. Cell proliferation, proteoglycan and glycosaminoglycan (GAG) production, and collagen production were assessed on days 3, 6, 10, 15 and 20. Results Chondrocy
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de Mesa, A. Arango, M. Cheyroux, and E. Edlinger. "Mise en évidence du virus herpès simplex (VHS) par effet cytopathogène en culture cellulaire, par immunofluorescence et par un test d'hybridation." Médecine et Maladies Infectieuses 15, no. 9 (1985): 515–17. http://dx.doi.org/10.1016/s0399-077x(85)80057-3.

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Lambros, Maria P., Lavanya Kondapalli, Cyrus Parsa, et al. "Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/425760.

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Qingre Liyan decoction (QYD), a Traditional Chinese medicine, and N-acetyl cysteine (NAC) have been used to prevent radiation induced mucositis. This work evaluates the protective mechanisms of QYD, NAC, and their combination (NAC-QYD) at the cellular and transcriptional level. A validated organotypic model of oral mucosal consisting of a three-dimensional (3D) cell tissue-culture of primary human keratinocytes exposed to X-ray irradiation was used. Six hours after the irradiation, the tissues were evaluated by hematoxylin and eosin (H and E) and a TUNEL assay to assess histopathology and apop
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Bao, Q., and R. C. Hughes. "Galectin-3 expression and effects on cyst enlargement and tubulogenesis in kidney epithelial MDCK cells cultured in three-dimensional matrices in vitro." Journal of Cell Science 108, no. 8 (1995): 2791–800. http://dx.doi.org/10.1242/jcs.108.8.2791.

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Galectin-3 is a member of a closely related family of beta-galactoside-binding soluble proteins found in many vertebrate epithelial and myeloid cell types. The developmentally regulated presence of galectin-3 in tissues, for example kidney, and an affinity for many cell-surface and matrix glycoproteins indicate its importance in extracellular biological processes. Since a polarised expression and secretion of galectin-3 was observed in monolayer-cultured MDCK cells, an understanding of the secretion and distribution of this lectin in a three-dimensional in vitro model would help to uncover its
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Saputra, Rahmana Febri, Arlina Dewi, Dyah Tri Kusuma Dewi, and Mariska Urhmila. "Analysis of 12-Dimensions of Patient Safety Culture at PKU Muhammadiyah Bantul Hospital (Plenary Accredited)." Mutiara Medika: Jurnal Kedokteran dan Kesehatan 24, no. 2 (2024): 23–31. http://dx.doi.org/10.18196/mmjkk.v24i2.22947.

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Hospital accreditation is very important as an investment in efforts to improve quality and patient satisfaction. Increasing awareness among health professionals about the importance of accreditation and encouraging participation in accreditation can improve the quality of the health service delivery system. Patient safety culture have an important role in the existence of hospital accreditation which can be a determinant in achieving the patient safety quality designation. This article aims to see the impact of accreditation on patient safety culture at PKU Muhammadiyah Bantul Hospital with p
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Banse, Emilie, Moïra Mikolajczak, Marie Bayot, Anne-Laure Lenoir, and Philippe de Timary. "Harmful dimensions of medical culture in relation to physician burnout: A cross-sectional study." PLOS Mental Health 2, no. 4 (2025): e0000301. https://doi.org/10.1371/journal.pmen.0000301.

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Physician burnout (PB) is an emotional exhaustion in response to long-term workplace stress. Despite growing interest in how the medical culture contributes to physician burnout, there is little empirical research on the subject. In this study, we aimed to evaluate quantitatively specific dimensions of the medical culture, thereby testing the hypothesis that a group of interdependent aspects of medical culture would positively correlate with PB. We collected on-line survey results in a cross-sectional study of 1002 physicians in current clinical practice. Participants completed the Burnout Ass
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Wei, Chunyang, Chengzhuang Yu, Shanshan Li, Tiejun Li, Jiyu Meng, and Junwei Li. "Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture." Biosensors 12, no. 5 (2022): 350. http://dx.doi.org/10.3390/bios12050350.

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Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex cellular organizations, heterogeneity, and cell–extracellular matrix (ECM) interactions. This article presents a droplet-based microfluidic chip that integrates cell distribution, 3D in vitro cell culture, and in situ cell monitoring in a single device. Using the microfluidic “co-flow step emulsification” approach, we have successfully prepared close-
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Ballestreri, Érica, Carlos Alexandre Fedrigo, Carlos Alexandre Fedrigo, et al. "Development of three-dimensional cell culture spheroid model for non-small-cell lung carcinoma cell line nci-h460 exposed to cisplatin / Desenvolvimento de modelo de cultura celular tridimensional para a linhagem celular de carcinoma de pulmão de não-pequenas células nci-h460 expostas á cisplatina." Brazilian Journal of Development 7, no. 12 (2021): 11140–115148. http://dx.doi.org/10.34117/bjdv7n12-331.

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The three-dimensional culture models have been developed to promote better simulation of in vivo conditions of cellular interactions and phenotypic expressions. Since each cell line behaves differently and not all aggregate three-dimensionally, the objectives of this study were to standardize the conditions for cultivation spheroids produced from the human non-smal-cell lung cancer cell line NCI-H460, and evaluate the effects of cisplatin in this model. We investigated the best cell density for the formation of spheroids and their growth time in culture conditions. For this, spheroids were tre
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Winley, Graham Kenneth, and Tipa Sriyabhand. "Culture in Thai Society and Online Virtual Communities." International Journal of Information Communication Technologies and Human Development 12, no. 1 (2020): 24–39. http://dx.doi.org/10.4018/ijicthd.2020010102.

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Cultural characteristics of an online virtual community (VC) are compared with cultural characteristics of Thai society (TS). Cultural characteristics are analyzed using Hofstede's dimensions and data from a sample of 369 Thai citizens who are active members of a VC. Also, associations between cultural characteristics and personal characteristics (gender, age, education, VC experience, and work position) were examined in both contexts. The findings indicate (1) individualism, masculinity, and indulgence are more evident in TS than in a VC; (2) in a VC, there were no significant differences bet
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Mulyati, Tatik. "PENGARUH KOMPETENSI, BUDAYA AKADEMIK DAN KEPEMIMPINAN SPIRITUAL TERHADAP MOTIVASI DAN IMPLIKASINYA." EKUITAS (Jurnal Ekonomi dan Keuangan) 19, no. 1 (2017): 66. http://dx.doi.org/10.24034/j25485024.y2015.v19.i1.1756.

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The purpose of this study describes competency, academic culture, spiritual leadership, motivation and lecturers’performance; to analyze the influence of competency, academic culture and spiritual leadership on motivation and to analyze the influence of competency, academic culture and spiritual leadership on lecturers’performance through motivation in Merdeka University in East Java. Using the ‘Structural Equation Modeling’ with 193 lectures as samples, this study has following results: (1) Competencies’ factor dimensions consist of pedagogical competency, professional competency, personality
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Zamora, Roberto, and Rosalinda Hernandez. "The Impact of Organizational Health on Student Achievement in a High Needs District." Journal of Studies in Education 6, no. 3 (2016): 149. http://dx.doi.org/10.5296/jse.v6i3.9904.

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This study establishes the relationship between organizational health and student achievement in English Language Arts and Mathematics in grades 3-11. The purpose of this quantitative study was to assess the relationship between student achievement as measured by student performance in the State of Texas Assessment of Academic Readiness and organizational health. The Spearman Rho correlation coefficient was computed to determine the strength of the relationships between student achievement and the ten dimensions of organizational health. The findings indicated there was a positive relationship
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Blyznyuk, Tetyana, and Oleksandr Bliznyuk. "Cross-cultural profile of a leader: the influence of national culture." Actual problems of innovative economy and law 2024, no. 3 (2024): 50–53. http://dx.doi.org/10.36887/2524-0455-2024-3-10.

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National culture directly affects people’s values, mentality, and dominant management style. Managers of companies in different countries now acutely feel the influence of the cultural context of the country where they work. Understanding and considering the cultural characteristics of various countries with representatives of which a particular company interacts positively affects the company’s management process, creating a synergistic effect in increasing the efficiency of its activities. The most comprehensive assessment of the impact of national culture on the personnel management of an o
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DAMELIN, L., S. CHOUDHURY, S. COWARD, M. HUBANK, H. HODGSON, and C. SELDEN. "1086 HEPG2 cells in 3-dimensional culture exhibit elevated expression of genes associated with lipid metabolism — further evidence that cells cultured in alginate can provide the cellular component of a bioartificial liver." Hepatology 38 (2003): 678. http://dx.doi.org/10.1016/s0270-9139(03)81124-2.

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Tanimizu, Naoki, Atsushi Miyajima, and Keith E. Mostov. "Liver Progenitor Cells Develop Cholangiocyte-Type Epithelial Polarity in Three-dimensional Culture." Molecular Biology of the Cell 18, no. 4 (2007): 1472–79. http://dx.doi.org/10.1091/mbc.e06-09-0848.

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Cholangiocytes are cellular components of the bile duct system of the liver, which originate from hepatoblasts during embryonic liver development. Although several transcription factors and signaling molecules have been implicated in bile duct development, its molecular mechanism has not been studied in detail. Here, we applied a three-dimensional (3D) culture technique to a liver progenitor cell line, HPPL, to establish an in vitro culture system in which HPPL acquire differentiated cholangiocyte characteristics. When HPPL were grown in a gel containing Matrigel, which contains extracellular
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Totty, Stephanie, Prajakta Kolte, and Stoney Brooks. "Why do People Share About Themselves Online? How Self-presentation, Work–home Conflict, and the Work Environment Impact Online Self-disclosure Dimensions." Communications of the Association for Information Systems 55 (2024): 875–96. https://doi.org/10.17705/1cais.05534.

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Online self-disclosure (OSD) allows people to build and maintain relationships with others, which has become increasingly important as more work has become remote in recent years. Previous research has investigated OSD as a higher-order factor. We propose that the OSD dimensions—amount, depth, honesty, intention, and valence—may be impacted differently by its antecedents. Specifically, we investigate the relationship between two theorized antecedents of OSD—self-presentation and work-home conflict—and the dimensions of OSD. Additionally, we posit that organizational culture factors may affect
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Arun, Korhan, Cem Şen, and Olcay Okun. "How does Leadership Effectiveness related to the Context? Paternalistic Leadership on non-financial Performance within a cultural Tightness- Looseness Model?" Journal of East European Management Studies 25, no. 3 (2020): 503–29. http://dx.doi.org/10.5771/0949-6181-2020-3-503.

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Current emphasis in culture research focuses on how leaders might change the culture to improve organizational performance. However, how culture affects organizational performance under active leadership relations research has resulted in conceptual ambiguities, as well as contradictory empirical findings. We argue that organizational culture moderates the effectiveness of leadership on organizational performance. We used an ethical approach to generalize paternalistic leadership in moderating Turkish organizational culture. The results indicate that paternalistic leadership functions are divi
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Leicht, Lisa, Tilmann Wendel, and Maximilian Wolf. "Implementierung von agiler Führung in multinationalen Konzernen: Empirische Erkenntnisse aus der Halbleiterbranche." Leadership, Education, Personality: An Interdisciplinary Journal 2, no. 1 (2020): 41–51. http://dx.doi.org/10.1365/s42681-020-00012-3.

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Abstract This paper provides empirical insights and practical implications for the implementation process of agile leadership in multinational firms. Building on Häußler’s and Fischer’s Trafo-model our research shows that the dimensions “culture” and “leadership” have been important dimensions for the Advantest Europe GmbH in 2017 to boost the implementation of agile leadership. Concerning the dimension “culture” the most important insight was that agile leadership is more than agile methods such as Scrum or Kanban. Concerning the dimension “leadership” our results show for example that a lack
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Elbaz, Jamal, Sadia Iddik, and Mohamed Oubal. "The effects of Cultural factors on GSCM implementation, Empirical evidence from Morocco." SHS Web of Conferences 119 (2021): 03002. http://dx.doi.org/10.1051/shsconf/202111903002.

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This paper aims to link Green SCM and cultural factors by empirically testing a conceptual model emphasizing how national culture and organizational culture affect the GSCM implementation. The conceptual model includes the effects of the firm’s characteristics, especially the firm size, type and industry sectors, on the application of GSCM practices. This paper was conducted among a sample of manufacturing companies incorporating a range of industrial sectors from Morocco. The data was collected using an online questionnaire and analyzed using SPSS version 25 and SmartPLS software 3. The findi
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Wang, I.-Ming, Chich-Jen Shieh, and Fu-Jin Wang. "EFFECT OF HUMAN CAPITAL INVESTMENT ON ORGANIZATIONAL PERFORMANCE." Social Behavior and Personality: an international journal 36, no. 8 (2008): 1011–22. http://dx.doi.org/10.2224/sbp.2008.36.8.1011.

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Based on 150 valid questionnaires, an investigation was undertaken through correlation analysis and multiple regression analysis to examine the following: the correlation between human capital investment and organizational performance, between organizational culture and human capital investment, between organizational culture and organizational performance, and finally the effect of organizational culture on the correlation between human capital investment and organizational performance. The relationship between staff training and development and internal trust relations positively correlated
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Abriszewski, Krzysztof. "Uczestnictwo jednostek w kulturze. Uwagi na temat nowej dynamiki kultury na przykładzie melomanów i ewolucji nośników muzyki." Kultura i Społeczeństwo 55, no. 1 (2011): 41–62. http://dx.doi.org/10.35757/kis.2011.55.1.3.

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The aim of the text is to sketch the dynamics of contemporary culture, focussing on the concept of participation. General remarks are accompanied by illustrations taken from ethnographic study on music lovers faced with evolution of musical media (vinyl records, CDs, files). Following Marek Krajewski, the author rejects the traditional notion of “participation”, and uses his redefinition of the term. He stresses three dimensions of cultural participation: 1. participation as socialization; 2. participation as reshaping the participants themselves, which makes material traces; 3. participation
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Shirolkar, Shweta, and Kanchan Patil. "ONLINE FOOD DELIVERY APPLICATIONS: QUALITATIVE INQUIRY OF DIMENSIONS IMPACTING CONSUMER INTENTIONS AND USAGE." New Trends in Qualitative Research 21, no. 1 (2025): e1043. https://doi.org/10.36367/ntqr.21.1.2025.e1043.

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The study explores different dimensions impacting Food Delivery apps' usage in Tier 1, 2, and 3 cities in India. The study highlights the barriers along with drivers of usage and adoption of online food delivery applications. Research Questions for the current inquiry are 1) What dimensions impact the adoption of online food delivery aggregators’ services in Tier 1, 2, and 3 cities? 2) What are the situation triggers, and barriers impacting the usage of online food delivery aggregator’s services in Tier 1, 2, and 3 cities? A qualitative approach is used to collect the data. In-depth interviews
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