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Relevant bibliographies by topics / Culture-independent techniques

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Journal articles
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Academic literature on the topic 'Culture-independent techniques'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 February 2022

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Journal articles on the topic "Culture-independent techniques"

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Hirsch, Penny R., Tim H. Mauchline, and Ian M. Clark. "Culture-independent molecular techniques for soil microbial ecology." Soil Biology and Biochemistry 42, no. 6 (2010): 878–87. http://dx.doi.org/10.1016/j.soilbio.2010.02.019.

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Lazarevic, Vladimir, Katrine Whiteson, Nadia Gaïa, et al. "Analysis of the salivary microbiome using culture-independent techniques." Journal of Clinical Bioinformatics 2, no. 1 (2012): 4. http://dx.doi.org/10.1186/2043-9113-2-4.

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Vaughn, Andrew, Courtney Shaver, and David Clark. "Association Between Culture and Culture-Independent Microtyping in Recalcitrant Chronic Rhinosinusitis." Ear, Nose & Throat Journal 98, no. 2 (2019): 94–97. http://dx.doi.org/10.1177/0145561318823371.

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Background: Many different etiologies have been proposed to be responsible for the pathogenesis of chronic rhinosinusitis, including dysbiosis of the sinus microbiome. Attempts have recently been made to identify a pathogenic organism via advanced culture mechanisms. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. Methods: Medical records were retrospectively reviewed for patients with a hist

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Willner, Dana, and Phil Hugenholtz. "Metagenomics and Community Profiling: Culture-Independent Techniques in the Clinical Laboratory." Clinical Microbiology Newsletter 35, no. 1 (2013): 1–9. http://dx.doi.org/10.1016/j.clinmicnews.2012.12.001.

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Knight, Stephen T., Vernon R. Neece, and Donald J. Witt. "Rapid culture-independent techniques for quantitation of Chlamydia trachomatis elementary bodies." Journal of Microbiological Methods 10, no. 4 (1989): 255–63. http://dx.doi.org/10.1016/0167-7012(89)90013-4.

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Andorrà, Imma, Sara Landi, Albert Mas, José M. Guillamón, and Braulio Esteve-Zarzoso. "Effect of oenological practices on microbial populations using culture-independent techniques." Food Microbiology 25, no. 7 (2008): 849–56. http://dx.doi.org/10.1016/j.fm.2008.05.005.

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Martinez, M. F., P. J. Shand, and D. R. Korber. "Evaluation of microbial dynamics on low-sodium bologna using culture-dependent and culture-independent techniques." Meat Science 96, no. 1 (2014): 120. http://dx.doi.org/10.1016/j.meatsci.2013.05.048.

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Afianti, Nur Fitriah, and Yeti Darmayati. "PENDEKATAN CULTURE INDEPENDENT UNTUK ANALISIS KOMUNITAS BAKTERI." OSEANA 42, no. 1 (2019): 9–17. http://dx.doi.org/10.14203/oseana.2017.vol.42no.1.34.

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CULTURE INDEPENDENT APPROACH FOR BACTERIAL COMMUNITY ANALYSIS. Analysis of bacterial community can be through two approaches, through cultivation (culture dependent) and without cultivation (culture independent). Culture dependent approach is conventional method which only covered few bacteria because not all bacteria could be cultured. Culture independent approach with molecular techniques based on DNA communities can provide more information about the structure and diversity of bacteria in nature, both culturable bacteria and unculturable bacteria. 16S rRNA gene is commonly target gene used

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Pearce, D. A., C. J. Gast, B. Lawley, and J. C. Ellis-Evans. "Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques." FEMS Microbiology Ecology 45, no. 1 (2003): 59–70. http://dx.doi.org/10.1016/s0168-6496(03)00110-7.

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Varela Villarreal, Jessica, Thomas Schwartz, and Ursula Obst. "Culture-independent techniques applied to food industry water surveillance — A case study." International Journal of Food Microbiology 141 (July 2010): S147—S155. http://dx.doi.org/10.1016/j.ijfoodmicro.2010.03.001.

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Dissertations / Theses on the topic "Culture-independent techniques"

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Adhikari, Deepak. "Microbial response to different carbon source amendments in agricultural soils as monitored by culture-independent techniques." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 127 p, 2008. http://proquest.umi.com/pqdweb?did=1605142671&sid=3&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Andorrà, Solsona Immaculada. "Adaptation and development of culture-independent techniques for the indentification and enumeration of microorganisms in wine fermentations." Doctoral thesis, Universitat Rovira i Virgili, 2010. http://hdl.handle.net/10803/8690.

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L'objectiu d'aquesta tesi va ser l'adaptació i validació de diferents tècniques independents de cultiu per a la detecció i quantificació de la microbiota present a la fermentació vínica. Es van assajar la QPCR (PCR quantitativa) per a la quantificació i seguiment d'espècies clau i la diversitat ecològica es va analitzar per DGGE (electroforesi en gel desnaturalitzant) i la clonació d'un fragment ribosomal.<br/>Tanmateix es va estudiar l'aplicació de la Hibridació in Situ (FISH) i la QPCR amb colorants específics per tal de diferenciar cèl·lules vives i mortes. <br/>Aquestes tècniques es varen

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Caleb, Oluwafemi James. "Microbial community structure as an indicator of soil health in apple orchards." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4133.

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Thesis (MSc (Microbiology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: The relationship between various land management practices, soil properties and the soil microbial communities are complex and little is known about the effect of these interactions on plant productivity in agricultural systems. Although it would be advantageous to have a single organism or property that can be used as a measure of soil health, it may not be possible. Soil organisms which include both the microorganisms as well as soil fauna are subjected to the effect of their immediate environment. This

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Leareng, Samuel Keeng. "Characterization and identification of microbial communities in pigeon droppings using Culture-Independent techniques." Thesis, 2015. http://hdl.handle.net/10352/349.

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M. Tech. (Department of Biotechnology, Faculty of Applied and Computer Science), Vaal University of Technology|<br>Pigeon droppings, found in abundance in most cities and towns where pigeons are found, are a source of potential yeast and molds into the environment. Invasive fungal infections are a cause of morbidity and often mortality in immunocompromised individuals. The objective of this study was to the identification of bacterial and mold agents from pigeon droppings. Pigeon droppings samples were collected from three locations during the winter and summer months and studied for the occur

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Lekhanya, Lebohang Lieketseng. "Bacterial diversity of soil irrigated with Gypsiferous mine water as determined by culture-dependent and -independent techniques." Diss., 2010. http://hdl.handle.net/2263/29438.

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In the past, the response of microbial populations to anthropogenic disturbances was studied using conventional methods based on cultivation of microorganisms and on measurement of their metabolic activities (Fantroussi et al., 1999). However, these culturing methods often account for a small proportion of the total microbial community (Ibekwe and Kennedy, 1998; Hill et al., 2000). To overcome this, molecular techniques were developed and these allowed for the analyses of microorganisms in their natural habitats. Analysis of the 16S rRNA molecule and its corresponding gene (16S rDNA) has been

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Book chapters on the topic "Culture-independent techniques"

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Sasidharan, Santanu, K. Divakar, and Prakash Saudagar. "Culture-Independent Omics-Techniques for Microbiome-Based Molecular Therapeutics Against Infectious Diseases." In Metagenomic Systems Biology. Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-8562-3_5.

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Ercolini, D., and L. Cocolin. "IDENTIFICATION METHODS | Culture-Independent Techniques." In Encyclopedia of Food Microbiology. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-384730-0.00438-9.

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P. Singh, Akhlash. "Genomic Techniques Used to Investigate the Human Gut Microbiota." In Human Microbiome. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.91808.

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The human gut is the complex microbial ecosystem comprises more than 100 trillion microbes also known as microbiota. The gut microbiota does not only include about 400–500 types of bacterial strains, but it also contains archaea, bacteriophage, fungi, and protozoa species. In order to complete the characterization of the gut microbial community, we need the help of many culture-dependent and culture-independent genomic technologies. Recently, next-generation sequencing (NGS), mediated metagenomics that rely on 16S rRNA gene amplification, and whole-genome sequencing (WGS) have provided us deep knowledge related to important interactions such as host-microbiota and microbe-microbe interactions under various perturbation inside the gut. But, we still lack complete knowledge related to unique gene products encoded by gut meta-genome. Hence, it required the application of high-throughput “omics-based” methods to support metagenomics. Currently, a combination of high-throughput culturing and microfluidics assays is providing a new method to characterize non-amenable bacterial strains from the gut environment. The recent additions of artificial intelligence and deep learning to the area of microbiome studies have enhanced the capability of identification of thousand microbes simultaneously. Given above, it is necessary to apply new genome editing tools that can be used to design the personalized microflora which can be used to cure lifestyle-related diseases.

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Bohlman, Andrea F. "Sound." In Musical Solidarities. Oxford University Press, 2020. http://dx.doi.org/10.1093/oso/9780190938284.003.0002.

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Chapter 1 lays out the tension between ideas about music’s political efficacy and the productive work that sound did for the opposition in Poland. Within the intellectual community that shaped much of dissident culture, poets, filmmakers, and actors were more engaged with politics than composers and musical performers. To understand the logic behind claims that music was less politically meaningful, the chapter compares the Communist Party’s policies toward music with those debated in the oppositional journal Independent Culture. In contrast, the opposition’s cassette culture circumvented the Censorship Bureau and stumped secret police surveillance, revealing the powerful political potential of sound. Its founders were invested in tape contra print as well as cassettes’ transnational distribution and alternative economies. Listening to these cassettes as sounding artifacts reveals diverse repertories and creative editing techniques that belie the assumption that music was politically impotent for the opposition.

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Klaubauf, Sylvia, and Frank J. J. Segers. "Research Tools and Methods for the Analysis of Microbiota in Dairy Products." In Microbial Cultures and Enzymes in Dairy Technology. IGI Global, 2018. http://dx.doi.org/10.4018/978-1-5225-5363-2.ch002.

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Microorganisms play important roles in dairy products. They can be a source of spoilage, or they promote health or cause diseases. In dairy fermentations, microorganisms are of great importance due to their function as starter cultures and during the production process of dairy products. In order to better understand and improve the process, it is essential to identify the species that are involved and to monitor the development of microbial communities. There are several different approaches for the detection and analysis of the microbiota. The methods can be culture dependent and, for example, make use of morphological and physiological characters or DNA sequencing. Culture-independent methods include direct PCR and qPCR, but also electrophoresis-based profiling techniques as well as metagenomics. Descriptions of relevant methods are provided and their applications are discussed in this chapter.

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Hoof, Florian. "Introduction." In Angels of Efficiency, translated by Daniel Fairfax. Oxford University Press, 2020. http://dx.doi.org/10.1093/oso/9780190886363.003.0001.

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This introductory chapter establishes the book’s goal of recovering the media history of consulting and determining what the global growth of consulting knowledge can tell us about the modern world. The time span of the investigation, from 1880 to 1930, covers three parallel developments, which lead to a fundamental transformation of industrial knowledge structures. First, there is the constitution of an independent form of managerial activity in industry. Second, there is the establishment of the field of corporate consulting. Third, there is the emergence of a series of visualization techniques after 1880, which are at the disposal of the first two spheres, management and corporate consulting. These three tropes lead to a new form of visual management that follows from oral and written forms of management. The introduction describes the interdisciplinary approach the author adopts to trace the visual culture and historical epistemology of business consulting and consulting knowledge between media and business history and theory.

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Pennisi, Giuseppina, Alessandro Pistillo, Elisa Appolloni, Francesco Orsini, and Giorgio Gianquinto. "Advanced hydroponics design for plant cultivation in cities." In Burleigh Dodds Series in Agricultural Science. Burleigh Dodds Science Publishing, 2021. http://dx.doi.org/10.19103/as.2020.0076.13.

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The growth of the world urban population altogether with the detrimental effects of climate change and resource scarcity are currently exerting extreme pressure on our food systems. Innovation in vegetable crop production is being driven by plant cultivation technologies that are independent of soil fertility and availability, highly efficient in the use of water and mineral nutrients and adapted to protected environments with resilience to both biotic and abiotic stresses. Soilless culture systems (SCS) are most suited to tackle these challenges, and in recent years their innovation has mainly targeted the adaptation of the growing techniques developed in traditional commercial greenhouse systems into advanced hydroponic designs fitted to diverse urban environments. This chapter describes the most recent innovations in SCS for plant cultivation within urban settings. These include rooftop farms and cultivation inside buildings through the use of artificial lighting. Finally, the chapter looks ahead to future research trends in this area.

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"Research Methodology, Questionnaire, Sampling, Data Analysis Techniques." In Organizational Culture and Achieving Business Excellence. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-8413-1.ch004.

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The previous chapter developed the research model and hypotheses. This chapter now explains the methodology. It gives more information on the research variables, including the independent, dependent, and moderator variables, and discusses the research sample size and sampling frame, together with the justification for choice of sampling technique. It describes the pilot study and reports on the reliability of the scales chosen. It explains the data collection procedure for the main study, including activities related to the administration of the research questionnaire and communication with the target companies, and provides details of the number of responses received from each company and the response rate. It also covers ethical considerations, including the actions taken to maintain the respondents' anonymity and protect data from unauthorized use.

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"Fluorescent antibody techniques have allowed for the direct identification and enumeration of individual bacteria in environmental samples without requiring prior growth in culture media (Bahlool and Schmidt 1980, Cloete and Steyn 1988, Macario et al. 1989). The technique involves the use of specific antibodies raised against surface markers of defined pure cultures that are either labelled directly with fluorescent dye molecules or via a fluorescent secondary antibody. This approach has yielded important insights into the spatial distribution of microorganisms, but it suffers from a number of disadvantages. For example, expression of the antigen may be influenced by environmental factors; false-positive and false-negative results may be obtained due to cross-reactivity or lack of reaction; non-specific binding of antibodies may result in high levels of background fluorescence; and production of specific antibodies requires a pure culture of the organism of interest (Cloete and de Bruyn Various recombinant DNA techniques have subsequently been developed that are independent of cultivation methods (Fig. 1). These techniques provide ways of detecting and quantifying specific phylogenetic groups of microbes on 16S rDNA sequences, and relevant structural genes provide ways of monitoring microbial populations of environmental and industrial systems. In addition to these tools, a number of emerging technologies such as the use of biomarker genes are being increasingly used to monitor with great precision and accuracy the behaviour of microbes in the environment." In Recent Advances in Marine Biotechnology, Vol. 8. CRC Press, 2003. http://dx.doi.org/10.1201/9781482279986-12.

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Conference papers on the topic "Culture-independent techniques"

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Poliyevskiy, Sergey, Galina Yamaletdinova, Elena Tsoy, and Olga Grigorieva. "Health Code as Methodological Basis of Athlete Students Self-Recovery." In The Public/Private in Modern Civilization, the 22nd Russian Scientific-Practical Conference (with international participation) (Yekaterinburg, April 16-17, 2020). Liberal Arts University – University for Humanities, Yekaterinburg, 2020. http://dx.doi.org/10.35853/ufh-public/private-2020-73.

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The importance of independent recreational practice for student-athletes is increasing in today’s environment. Therefore, it’s appropriate to prepare relevant recommendations for students undergoing heavy physical loads. The study was mainly aimed at the elaboration of the content of the Health Code for athlete students to render it as a component and method of implementing a self-health system. The theoretical and empirical methods applied during the implementation of the study were as follows: theoretic-comparative analysis, summarisation of relevant data from scientific-methodological publi

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