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1

Van Anh, Le Thi, Tran Ngoc Anh Thu, and Nguyen Thi Dong Phuong. "Investigation of microalgae culture by autoflocculation methodologies." Vietnam Journal of Biotechnology 20, no. 3 (2022): 487–94. http://dx.doi.org/10.15625/1811-4989/17059.

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Harvesting of microalgae from their different cultivation media has pointed out challenges in resolving the problems of flocculation. These challenges must be faced with a suitable method for inducing flocculation that avoid or limit the microalgae’s contamination. This study developed the fundamental experiments with a support of chemicals and some bacteria strains inducing the flocculation of Chlorella vulgaris SAG 211-19. Particularly, the determination of minimum content of Mg2+, Ca2+, E. coli ATCC 85922 and Bacillus subtilis MT300405 was effectuated with co-cultivation of microalgae and s
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Wang, Hui, Haywood D. Laughinghouse, Matthew A. Anderson, et al. "Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1." Applied and Environmental Microbiology 78, no. 5 (2011): 1445–53. http://dx.doi.org/10.1128/aem.06474-11.

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ABSTRACTIncreasing petroleum costs and climate change have resulted in microalgae receiving attention as potential biofuel producers. Little information is available on the diversity and functions of bacterial communities associated with biofuel-producing algae. A potential biofuel-producing microalgal strain,Nannochloropsis oceanicaIMET1, was grown in Permian groundwater. Changes in the bacterial community structure at three temperatures were monitored by two culture-independent methods, and culturable bacteria were characterized. After 9 days of incubation,N. oceanicaIMET1 began to aggregate
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Noerdjito, Diah Radini. "PERKEMBANGAN, PRODUKSI, DAN PERAN KULTUR MIKROALGA LAUT DALAM INDUSTRI." OSEANA 42, no. 1 (2019): 18–27. http://dx.doi.org/10.14203/oseana.2017.vol.42no.1.35.

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DEVELOPMENT, PRODUCTION, AND THE ROLE OF MARINE MICROALGA IN INDUSTRY. Microalgae cultures recently have been used for various purposes. Culture development of microalgae started from the late 1800s and still continue until now. Specific media composition, lighting, aeration, culture system were applied to many different species microalgae for specific purposes such as pharmaceutical, healthcare, cosmetics, environmental management, energy production, and aquaculture.
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Gonzalez, Luz E., and Yoav Bashan. "Increased Growth of the Microalga Chlorella vulgariswhen Coimmobilized and Cocultured in Alginate Beads with the Plant-Growth-Promoting Bacterium Azospirillum brasilense." Applied and Environmental Microbiology 66, no. 4 (2000): 1527–31. http://dx.doi.org/10.1128/aem.66.4.1527-1531.2000.

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ABSTRACT Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacteriumAzospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigments significantly increased. Light microscopy revealed that both microorganisms colonized the same cavities inside the beads, though the microalgae tended to concentrate in the more aerated periphe
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Gonzalez-Bashan, Luz E., Vladimir K. Lebsky, Juan P. Hernandez, Jose J. Bustillos, and Yoav Bashan. "Changes in the metabolism of the microalga Chlorella vulgaris when coimmobilized in alginate with the nitrogen-fixing Phyllobacterium myrsinacearum." Canadian Journal of Microbiology 46, no. 7 (2000): 653–59. http://dx.doi.org/10.1139/w00-041.

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In an agroindustrial wastewater pond, a naturally occurring unicellular microalga, Chlorella vulgaris, was closely associated with the terrestrial plant-associative N2-fixing bacterium Phyllobacterium myrsinacearum. When the two microorganisms were artificially coimmobilized in alginate beads, they shared the same internal bead cavities, and the production of five microalgal pigments increased, but there were no effects on the number of the cells or the biomass of the microalga. The association, however, reduces the ability of C. vulgaris to remove ammonium ions and phosphorus from water. The
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Ghazali, Nurarina Ayuni, Nurul Aini Abdul Halim, Ninie Diana Baharuddin, Fatimah Md Yusoff, Murni Karim, and Ikhsan Natrah. "Ammonia removal and nitrogen preferences evaluation of indigenous Malaysian microalga Halamphora sp. on white shrimp Penaeus vannamei wastewater." Journal of Aquatic Research and Sustainability 01, no. 02 (2024): 10–17. http://dx.doi.org/10.69517/jars.2024.01.02.0003.

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Ammonia is ubiquitous in aquaculture systems and its removal is important for maintaining water quality and the health of the cultured animals. Microalgae are effective at removing ammonia from water, but the effectiveness of different microalgae species may vary. In this study, indigenous Malaysian microalgae isolated from shrimp ponds were screened for their ability in removing ammonia from synthetic culture media. The most efficient microalga’s nitrogen preferences and its growth and nitrogen removal in the early and late stages of shrimp culture wastewater were explored. It was found that
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7

Postaue, Najla, Leila Cristina Moraes, and Rosa Maria Farias Asmus. "CHORUME COMO FONTE DE NUTRIENTE NA PRODUÇÃO DA BIOMASSA MICROALGAL." e-xacta 12, no. 2 (2020): 11. http://dx.doi.org/10.18674/exacta.v12i2.2746.

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A biomassa de microalgas tem apresentado potencial para produção de biodiesel, contudo a viabilidade do cultivo de microalgas depende de fonte de nutrientes de baixo custo. O presente estudo objetivou utilizar o chorume como fonte de nutrientes para microalgas. Os experimentos foram conduzidos visando avaliar a obtenção da biomassa microalgal, conversão de lipídios e rendimento em ésteres metílicos de ácidos graxos, para os meios de cultivos utilizando 5%, 12% e 20% de chorume, com concentrações de 0,02, 0,05 e 0,08 g N. L-1 e para meio de controle contendo 1% de, Nitrogênio (N), Fósforo (P) e
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Barbara Araújo, Wesley Machado, Luiz Rodrigo Ito Morioka, Mayara Mari Murata, Josemeyre Bonifácio Da Silva, and Helio Hiroshi Suguimoto. "Uso de Microalgas como Bioestimuladoras da Germinação de Sementes." UNICIÊNCIAS 26, no. 1 (2022): 58–62. http://dx.doi.org/10.17921/1415-5141.2022v26n1p58-62.

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As microalgas possuem diversas aplicações biotecnológicas e dentro da agricultura podem ser utilizadas como biofertilizantes, bioestimuladoras da germinação de semente, biopesticidas e bioinsetisidas. Para estudar experimentalmente o uso da microalga Chlorella sorokiniana na germinação de sementes, é necessário obter informações na literatura sobre as metodologias de aplicação de microalgas em experimentos de campo que visem este processo. O presente trabalho teve como objetivo coletar informações por meio de uma revisão de literatura, sobre as metodologias e formas de aplicação das microalgas
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9

Pérez -Morales, A., A. Martínez -López, and J. M. Camalich -Carpizo. "DRY WEIGHT, CARBON, C/N RATIO, HYDROGEN, AND CHLOROPHYLL VARIATION DURING EXPONENTIAL GROWTH OF SELECTED MICROALGAE SPECIES USED IN AQUACULTURE." CICIMAR Oceánides 30, no. 1 (2015): 33. http://dx.doi.org/10.37543/oceanides.v30i1.168.

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Microalgae are commonly used as food source in aquaculture, mainly for shellfish and larvae of crustacean and fish. All hatcheries need an excellent inoculum to produce high-quality microalgae when cultured outdoor in extensive systems, and this depends largely on the health of the microalgae cultured under laboratory conditions as a primary step. Therefore, the aim of this work was to assess variations of dry weight, carbon, C/N ratio, hydrogen and chlorophylls as physiological indicators of nutrients uptake and growth rate during exponential growth of Isochrysis galbana, Chaetoceros calcitra
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10

Pérez -Morales, A., A. Martínez -López, and J. M. Camalich -Carpizo. "DRY WEIGHT, CARBON, C/N RATIO, HYDROGEN, AND CHLOROPHYLL VARIATION DURING EXPONENTIAL GROWTH OF SELECTED MICROALGAE SPECIES USED IN AQUACULTURE." CICIMAR Oceánides 30, no. 1 (2015): 33. http://dx.doi.org/10.37543/oceanides.v30i1.168.

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Microalgae are commonly used as food source in aquaculture, mainly for shellfish and larvae of crustacean and fish. All hatcheries need an excellent inoculum to produce high-quality microalgae when cultured outdoor in extensive systems, and this depends largely on the health of the microalgae cultured under laboratory conditions as a primary step. Therefore, the aim of this work was to assess variations of dry weight, carbon, C/N ratio, hydrogen and chlorophylls as physiological indicators of nutrients uptake and growth rate during exponential growth of Isochrysis galbana, Chaetoceros calcitra
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11

Yu, Hyeonjung, Jaai Kim, Chaeyoung Rhee, Juhee Shin, Seung Gu Shin, and Changsoo Lee. "Effects of Different pH Control Strategies on Microalgae Cultivation and Nutrient Removal from Anaerobic Digestion Effluent." Microorganisms 10, no. 2 (2022): 357. http://dx.doi.org/10.3390/microorganisms10020357.

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This study investigated nutrient removal from anaerobic digestion effluent by cultivating mixed-culture microalgae enriched from anaerobic sludge under different pH conditions: RUC (uncontrolled), R7–8 (maintained at 7–8), and R<8 (maintained below 8). Significant amounts of NH4+-N were lost by volatilization in RUC cultures due to increased pH values (≤8.6) during the early period of cultivation. The pH control strategies significantly affected the biological NH4+-N removal (highest in R7–8), microalgal growth (highest in R7–8), biomass settleability (highest in R<8), and microalgal gro
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12

Pathom-aree, Wasu, Sritip Sensupa, Antira Wichaphian, et al. "An Innovative Co-Cultivation of Microalgae and Actinomycete-Inoculated Lettuce in a Hydroponic Deep-Water Culture System for the Sustainable Development of a Food–Agriculture–Energy Nexus." Horticulturae 10, no. 1 (2024): 70. http://dx.doi.org/10.3390/horticulturae10010070.

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In recent years, researchers have turned their attention to the co-cultivation of microalgae and plants as a means to enhance the growth of hydroponically cultivated plants while concurrently producing microalgal biomass. However, the techniques used require precise calibration based on plant growth responses and their interactions with the environment and cultivation conditions. This study initially focused on examining the impact of hydroponic nutrient concentrations on the growth of the microalga Chlorella sp. AARL G049. The findings revealed that hydroponic nutrient solutions with electric
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Ermis, Hande, Ünzile Güven-Gülhan, Tunahan Çakır, and Mahmut Altınbaş. "Microalgae growth and diversity in anaerobic digestate compared to synthetic media." Biofuel Research Journal 9, no. 1 (2022): 1551–61. http://dx.doi.org/10.18331/brj2022.9.1.2.

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Economizing microalgal cultivation is a considerable milestone targeted by efforts put into microalgal biorefineries. In light of that, the present study was aimed to explore the potential of using anaerobic liquid digestate (ALD) as culture media to grow microalgae and compared it with three different synthetic media (i.e., N8, BBM, and M8) in terms of biomass yield, fatty acid composition, and nutrient utilization/recovery. Moreover, a mixed culture of wild-type microalgae was employed in this study owing to the ability of mixed cultures to survive extreme conditions, eliminating the risk of
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14

Journal, Baghdad Science. "Microalgae Chlorella Vulgaris Harvesting Via Co-Pelletization with Filamentous Fungus." Baghdad Science Journal 15, no. 1 (2018): 31–36. http://dx.doi.org/10.21123/bsj.15.1.31-36.

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The objective of this study was to progress another method for coagulation/flocculation of the microalga Chlorella vulgaris via pellet-forming of the fungal species Aspergillus niger which was isolated from municipal wastewater mud and the facultative heterotrophic microalga "C.vulgaris was used. The main factors studies were spore inoculums, organic carbon concentration in medium as well as pH variation which had considerably positive effects on microalgae/fungi co-pelletization formation. The process parameters are an inoculum1×104 spores/ML, 15 g/l sucrose as carbon source and pH ranged fro
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15

Wai Yan Cheah. "Microalgae Biorefinery from Palm Oil Mill Effluent (POME): Bioremediation and Biomass Production." Journal of Information Systems Engineering and Management 10, no. 33s (2025): 502–6. https://doi.org/10.52783/jisem.v10i33s.5560.

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Palm oil processing in Malaysia produces massive amounts of palm oil mill effluent (POME) every day. Effective treatment of POME is in necessity for compliance of regulations and for environmental sustainability. There is a new innovation in effluent treatment which is the application of microalgae for wastewater treatment and biomass production. The present study applied microalgae isolated from POME for the biomass production and bioremediation. The microalgae isolated are identified as Chlorella sorokiniana. Microalga was cultured in bold basal medium (BBM) for 20 days in a total volume of
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16

Ansilago, Mônica, Franciéli Ottonelli, and Emerson Machado de Carvalho. "Metals bioremediation potential using Pseudokirchneriella subcapitata." Revista Brasileira de Ciências Ambientais (Online) 56, no. 2 (2021): 223–31. http://dx.doi.org/10.5327/z21769478834.

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Microalgae are unicellular organisms, photosynthesizers that present cell duplication exponentially and biosorption capacity of nutrients dissolved in water. The objective of this work was to evaluate the capacity of the microalga Pseudokirchneriella subcapitata for bioremediation of metals and salts. In this aspect, the reduction of the metals and salts in the synthetic effluents by the microalga P. subcapitata was evaluated: (T1) culture medium (control); (T2) culture medium contaminated with aluminum chloride; (T3) culture medium contaminated with ferrous sulfate; (T4) culture medium contam
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Sauvage, Justine, Gary H. Wikfors, Xiaoxu Li, et al. "Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species." Applied Microbiology and Biotechnology 105, no. 5 (2021): 2139–56. http://dx.doi.org/10.1007/s00253-021-11147-8.

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Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros cal
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18

Yee, Chin Sze, Victor Tosin Okomoda, Fakriah Hashim, et al. "Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity." PeerJ 9 (April 23, 2021): e11217. http://dx.doi.org/10.7717/peerj.11217.

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This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only Thalassiosira weissflogii, Chlamydomonas sp. and Chlorella vulgaris were propagated successfully in Conway medium. Hence, these species were selected for the experiment comparing microalgae axenic culture and co-culture with the floc-forming bacterium, Bacillus infantis. Results obtained showed that the co-culture had higher microalgae biomass compared to the axenic culture. A similar trend was also observed concerning the lipid content of the
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Ajala, E. O., M. A. Ajala, G. S. Akinpelu, and V. C. Akubude. "Cultivation and Processing of Microalgae for Its Sustainability as a Feedstock for Biodiesel Production." Nigerian Journal of Technological Development 18, no. 4 (2022): 322–43. http://dx.doi.org/10.4314/njtd.v18i4.8.

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Microalgae are becoming sustainable alternative feedstocks to food crops for biodiesel production which can also solve the problems associated with the use of fossil fuels. However, several challenges about microalgae’s cultivation, harvesting, pre-treatment and extraction processes as well as the technology of biodiesel production affect its sustainability. This study proffers solutions to these challenges and recommended that hybrid culture systems with genetically engineered microalgal species would overcome the challenges of cultivation. The coagulation/flocculation method was adjudged the
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Zan, Zhaohui, Xinxin Huang, Zahid Hussain, et al. "Effects of Culture Medium Enrichment with Zinc on Astaxanthin Accumulation in a New Strain of the Microalga Dysmorphococcus globosus." Plants 13, no. 23 (2024): 3338. http://dx.doi.org/10.3390/plants13233338.

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High Zn2+ concentrations in microalgal cells that produce astaxanthin as a feed additive can reduce the symptoms of malnutrition in aquatic animals. Therefore, in this study, we analysed the effect of Zn2+ in the culture medium on the growth of a newly isolated microalgal strain Dysmorphococcus globosus ZY24. Zn2+ and white light stress altered the pigment content in microalgal cells. In addition, high Zn2+ concentrations in the culture medium altered cell morphology and chlorophyll fluorescence and also increased intracellular Zn2+ accumulation. Further, an optimal Zn2+ concentration in the c
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Reis, Daiane Felix, Francisco Roberto da Silva Machado Junior, Joana Da Costa Ores, Ailton Cesar Lemes, Carlos Andre Veiga Burkert, and Janaina Fernandes de Medeiros Burkert. "Influência do CO2 no Crescimento de Haematococcus Pluvialis e na Produção de Carotenoides." UNICIÊNCIAS 22, no. 3Esp (2019): 25. http://dx.doi.org/10.17921/1415-5141.2018v22n3espp25-29.

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O crescimento celular da microalga de água doce Haematococcus pluvialis e a bioprodução de carotenoides são influenciados pelas diferentes condições de cultivo, como deficiência de nutrientes, iluminância, aeração, agitação, temperatura e pH, alterando sua morfologia celular e produzindo cistos avermelhados (carotenogênese). A aeração nos cultivos de microalgas está relacionada a alguns fatores que influenciam no crescimento celular. As microalgas absorvem e utilizam CO2 como a principal fonte de carbono no crescimento celular. Logo, a biossíntese de pigmentos pode ocorrer pela limitação do ni
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Jeevanandam, Jaison, Mohd Razif Harun, Sie Yon Lau, Divine D. Sewu, and Michael K. Danquah. "Microalgal Biomass Generation via Electroflotation: A Cost-Effective Dewatering Technology." Applied Sciences 10, no. 24 (2020): 9053. http://dx.doi.org/10.3390/app10249053.

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Microalgae are an excellent source of bioactive compounds for the production of a wide range of vital consumer products in the biofuel, pharmaceutical, food, cosmetics, and agricultural industries, in addition to huge upstream benefits relating to carbon dioxide biosequestration and wastewater treatment. However, energy-efficient, cost-effective, and scalable microalgal technologies for commercial-scale applications are limited, and this has significantly impacted the full-scale implementation of microalgal biosystems for bioproduct development, phycoremediation, and biorefinery applications.
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Masojídek, Jiří, Karolína Štěrbová, Victor A. Robles Carnero, et al. "Photosynthetic Activity Measured In Situ in Microalgae Cultures Grown in Pilot-Scale Raceway Ponds." Plants 13, no. 23 (2024): 3376. https://doi.org/10.3390/plants13233376.

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The microalga Scenedesmus sp. (Chlorophyceae) was cultured in a raceway pond (RWP) placed in a greenhouse. The objective of this case study was to monitor the photosynthesis performance and selected physicochemical variables (irradiance, temperature, dissolved oxygen concentration) of microalgae cultures in situ at various depths of RWP. The data of actual photochemical yield Y(II), the electron transport rate monitored by in vivo chlorophyll fluorescence and photosynthetic oxygen production both in situ and ex situ revealed that (i) even in diluted cultures (0.6 g DW L−1), the active photic l
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Zhang, Xin Ru, Ze Yi Jiang, Hao Yuan, et al. "Influence of Surface Zeta Potential on Adhesion of Chlorella sp. to Substratum Surfaces." Advanced Materials Research 690-693 (May 2013): 1431–34. http://dx.doi.org/10.4028/www.scientific.net/amr.690-693.1431.

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Microalgae, in wet conditions, tend to grow on surfaces and form biofilms. The adhesion of microalgae to surfaces is very important for algal mass culture. The formation and development of microalgal biofims are in large denpend on the properties of cell surface, substratum surface and gowth medium. In this paper, the influence of substratum surface zeta potential on the microalgal biofilms was particularly investigated. We focused on a widely-used microalgal strain, the freshwater autotrophicChlorella sp..The adhesion phenomena ofChlorella sp.to surfaces with different zeta potential were obs
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Kovalev, N. N., S. E. Leskova, E. V. Mikheev, Yu M. Pozdnyakova, and R. V. Esipenko. "CULTURAL AND BIOCHEMICAL PARAMETERS OF TWO MICROALGAE, PHAEODACTYLUM TRICORNUTUM AND TETRASELMIS SUECIACA, IN CUMULATIVE CULTURES." Bulletin оf Kamchatka State Technical University, no. 53 (2020): 54–65. http://dx.doi.org/10.17217/2079-0333-2020-53-54-65.

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A comparative study of cultural and biochemical parameters of two microalgal species, Tetraselmis sueciaca and Phaeodactylum tricornutum, was performed. Cell density in the storage culture was 2.88 million cells per one ml for T. sueciaca and 20.37 million cells per one ml for Ph. tricornutum. As shown, determination of op-tical density of the culture medium is a more objective characteristic of the cumulative culture growth. For T. sueciaca, cell growth rate during the course of experiment made 400% and for Ph. tricornutum – 700%. Dif-ference in the chemical composition of microalgal biomass
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Borisova, Elena V., and Petro M. Tsarenko. "Microalgae culture collection of Ukraine." Algological Studies/Archiv für Hydrobiologie, Supplement Volumes 110 (November 1, 2003): 143–50. http://dx.doi.org/10.1127/1864-1318/2003/0110-0143.

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27

Wang, Jing Han, Hai Zhen Yang, and Feng Wang. "Potential of Mixotrophic Cultivation of Chlorella sorokiniana for Biodiesel Production." Advanced Materials Research 779-780 (September 2013): 1509–13. http://dx.doi.org/10.4028/www.scientific.net/amr.779-780.1509.

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Biodiesel from microalgae provides a promising alternative for biofuel production. Microalgal biodiesel can be produced under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Studies of biodiesel production from microalgae have been reported mostly focusing on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity ofChlorella sorokinianaunder photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was adopted as organic
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28

López-Pacheco, Itzel Y., Victoria Guadalupe Ayala-Moreno, Catherinne Arlette Mejia-Melara, et al. "Growth Behavior, Biomass Composition and Fatty Acid Methyl Esters (FAMEs) Production Potential of Chlamydomonas reinhardtii, and Chlorella vulgaris Cultures." Marine Drugs 21, no. 8 (2023): 450. http://dx.doi.org/10.3390/md21080450.

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The production of biomolecules by microalgae has a wide range of applications in the development of various materials and products, such as biodiesel, food supplements, and cosmetics. Microalgae biomass can be produced using waste and in a smaller space than other types of crops (e.g., soja, corn), which shows microalgae’s great potential as a source of biomass. Among the produced biomolecules of greatest interest are carbohydrates, proteins, lipids, and fatty acids. In this study, the production of these biomolecules was determined in two strains of microalgae (Chlamydomonas reinhardtii and C
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29

Fitriyah, F., Y. Faramitha, D. A. Sari, I. Kresnawaty, T. Panji, and D. Santoso. "Improved direct lysis PCR amplification method of microalgal culture for sequencing and species identification." IOP Conference Series: Earth and Environmental Science 948, no. 1 (2021): 012013. http://dx.doi.org/10.1088/1755-1315/948/1/012013.

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Abstract Molecular approach plays important role in species identification for microalgae which involves sequencing of specific DNA barcode present in the genome. This approach involved preparation of template DNA for polymerase chain reaction (PCR) which is time consuming and requires large amounts of algal cells. Microalgal direct PCR have been used frequently for species identification, which simplified the DNA isolation procedure. However, the recent attempts to amplify the rbcL gene of microalga using the previously reported protocol led to poor repeatability. In this study, Nannochlorops
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Vázquez, María, Juan Luis Fuentes, Adriana Hincapié, Inés Garbayo, Carlos Vílchez, and Maria Cuaresma. "Selection of microalgae with potential for cultivation in surfactant-stabilized foam." Algal Research 31 (February 6, 2018): 216–24. https://doi.org/10.5281/zenodo.14777373.

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Recently, microalgal cultivation in liquid foams has been developed. Compared to the traditional systems, thisconcept is expected to offer advantages such as increased mass transfer and reduced biomass harvesting costsand water consumption. However, there is limited information, thus far, on the microalgal performance in foambed photobioreactors. Therefore, this study was aimed at comparing the foaming properties of six algal strains to identify the criteria that could be broadly employed for assessing the microalgal potential for cultivation in a foam-bed photobioreactor. The m
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Nguyen, Thanh Tien, Yoshimitsu Uemura, Vijendren Krishnan, and Nadila Abdul Khalid. "Autoflocculation of Scenedesmus Quadricauda: Effects of Aeration Rate, Aeration Gas CO2 Concentration and Medium Nitrogen Concentration." International Journal of Biomass and Renewables 6, no. 1 (2017): 11. http://dx.doi.org/10.61762/ijbrvol6iss1art4546.

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After microalgal cultivation step, a recent method to harvest microalgae is adding flocculant into microalgal liquid culture to cause flocculation. Flocculated cells are easy to concentrate since their sedimentation time and successive filtering time are expected to be shorter. In this sense, autoflocculation of microalgae may be a beneficial phenomenon for easing the harvesting process of microalgae. In this study, autoflocculation was investigated for the growth of Scenedesmus quadricauda, a type of fresh water microalgae, at 25 ºC in a 5-L column photobioreactor. The BG11 medium was used. T
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Ma, Wei, Chenchen Feng, Fachun Guan, Dianrong Ma, and Jinling Cai. "Effective Chlorella vulgaris Biomass Harvesting through Sulfate and Chloride Flocculants." Journal of Marine Science and Engineering 11, no. 1 (2022): 47. http://dx.doi.org/10.3390/jmse11010047.

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Efficient microalgae harvesting is a great challenge hindering diverse industrial applications of microalgae. Flocculation is regarded as an effective and promising technology for microalgae harvesting. In this study, sulfate (Al2(SO4)3 and Fe2(SO4)3) and chloride flocculants (AlCl3 and FeCl3) were used to harvest Chlorella vulgaris. Flocculation conditions, including flocculant dose, flocculation time, stirring speed, stirring time, and flocculation pH, were optimized, and flocculant effects on microalgal cell status, floc characteristics, biomass composition, algal cell re-culture, and media
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Pinto, Jordan, Raphaël Lami, Marc Krasovec, et al. "Features of the Opportunistic Behaviour of the Marine Bacterium Marinobacter algicola in the Microalga Ostreococcus tauri Phycosphere." Microorganisms 9, no. 8 (2021): 1777. http://dx.doi.org/10.3390/microorganisms9081777.

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Although interactions between microalgae and bacteria are observed in both natural environment and the laboratory, the modalities of coexistence of bacteria inside microalgae phycospheres in laboratory cultures are mostly unknown. Here, we focused on well-controlled cultures of the model green picoalga Ostreococcus tauri and the most abundant member of its phycosphere, Marinobacter algicola. The prevalence of M. algicola in O. tauri cultures raises questions about how this bacterium maintains itself under laboratory conditions in the microalga culture. The results showed that M. algicola did n
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Bertolini, Marta, and Fosca Conti. "Algae Culture Conditions and Process Parameters for Phycoremediation and Biomaterials Production." Environmental and Climate Technologies 26, no. 1 (2022): 1092–105. http://dx.doi.org/10.2478/rtuect-2022-0082.

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Abstract Climate change and increasing world population call for careful utilization of water and energy sources. Microalgae to treat wastewater in a coupled process to produce biofuels and other value-added products for human consumption are promising solutions. An analysis of culture parameters and cultivation processes is presented as essential to achieve economical sustainability from the algae. Results of the activity of microalgal strains in detoxification of wastewater are compared and discussed, particularly in remediation of nitrogen and phosphorous compounds, heavy metal, pharmaceuti
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Palanisamy, Karthick Murugan, Obaid Ahmad Bhat, Natanamurugaraj Govindan, Mohd Hasbi Ab. Rahim, and Gaanty Pragas Maniam. "Impact of biomass density on growth rates of Spirulina platensis under different light spectra." Maejo International Journal of Energy and Environmental Communication 5, no. 1 (2023): 1–5. http://dx.doi.org/10.54279/mijeec.v5i1.248275.

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Light is the core environmental factor that affects the growth and biomass production of microalgae. However, the high density of microalgae will lead to reduction of the growth rate of microalgae culture due to availability of light decreases. Therefore, this experiment was conducted with aim of determining the influence of biomass density on growth of Spirulina platensis under different density of culture and LED lights. The result found that, the growth rate Spirulina platensis was reduced under high biomass density (9:1) due to lacking light penetration into the culture. However, white LED
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Araújo, Fabíola Ornellas de, Reinaldo Giudici, and João José Martins Simões de Sousa. "CULTIVATION OF THE MICROALGAE CHLORELLA PYRENOIDOSA USING THE PROCESSES OF BIOTECHNOLOGY." Revista Eletrônica Acervo Científico 2 (March 26, 2019): 121. http://dx.doi.org/10.25248/reac.e121.2019.

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The results obtained here, show that the use of Chlorella pyrenoidosa microalgae and biotechnology, using the discontinuous process, presented satisfactory results. With this, the study of the microalga Chlorella sp. has proved to be important because it has a wealth of proteins, carbohydrates, amino acids, fatty acids, carotenoids, vitamins and minerals in its constitution, which may represent commercial importance. This research revealed the best results for obtaining a lipoprotein-rich biomass, taking into account three different culture media, calculations of cell concentration, cell produ
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Antecao, Walter, Johanna Obreque, and Roberto Ramos. "Evaluación de la capacidad de Auxenochlorella sp. (Trebouxiophyceae, Chlorophyta) para la remoción de cobre en soluciones sintéticas." Revista de Biología Marina y Oceanografía 59, no. 3 (2024): 228–33. https://doi.org/10.22370/rbmo.2024.59.3.4914.

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A study was designed with the purpose of preliminarily evaluating heavy metal removal capacity of the green microalga Auxenochlorella sp., exposing it to different concentrations of copper in synthetic solutions. The study was carried out in the laboratory of Aguamarina Spa Company. Auxenochlorella sp. microalgae. were kept under cryopreservation at -80 °C until the date of the experiment. Then they were thawed and reactivated to be maintained in the culture medium described by the supplying laboratory. The microalgae were transferred from the cryogenizer tube to a test tube and then to Erlenm
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Yue, Li Hong, and Yi Peng Yang. "Study on Light Intensity Attenuation Characteristics of Chlorella Sp. Culture and the Growth of Chlorella Sp. with the Gradient Light Illuminate Culture." Applied Mechanics and Materials 675-677 (October 2014): 86–89. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.86.

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Based on the photoautotrophic characteristics of the freshwater microalage, light intensity attenuation characteristics in the cell culture system for Chlorella sp. were studied. Micralgae was cultured with a constant and the gradient light illuminate culture conditions, the cell growth of Microlagae was detected respectively, By means of regression analysis, mathematical models of illuminance intensity attenuation are gotten under different culture conditions. Results show algae cell density under gradient light culture conditions is higher than constant light, so the gradient light is more s
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Kovalev, Nickolay N., Svetlana Е. Leskova, Evgeny V. Mikheev, and Ekaterina A. Barsova. "EVALUATION OF THE EFFECT OF AUXINS ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHAETOCEROS MUELLERI." Siberian Journal of Life Sciences and Agriculture 16, no. 3 (2024): 205–26. http://dx.doi.org/10.12731/2658-6649-2024-16-3-841.

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Background. The study of microalgae cultivation process is not only related to obtaining a wide range of biologically active substances. With the development of mariculture, some species of microalgae (including Chaetoceros muelleri) are of interest as food for mollusks, arthropods and holothuria. The aim of this study was to evaluate the effect of auxin hormones on quantitative and qualitative parameters Chaetoceros muelleri accumulative cultivation. Materials and methods. The object of research was the culture of microalgae Chaetoceros muelleri. The duration of the experiment was 7 days. Ind
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Barua, Visva Bharati, and Mariya Munir. "A Review on Synchronous Microalgal Lipid Enhancement and Wastewater Treatment." Energies 14, no. 22 (2021): 7687. http://dx.doi.org/10.3390/en14227687.

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Microalgae are unicellular photosynthetic eukaryotes that can treat wastewater and provide us with biofuel. Microalgae cultivation utilizing wastewater is a promising approach for synchronous wastewater treatment and biofuel production. However, previous studies suggest that high microalgae biomass production reduces lipid production and vice versa. For cost-effective biofuel production from microalgae, synchronous lipid and biomass enhancement utilizing wastewater is necessary. Therefore, this study brings forth a comprehensive review of synchronous microalgal lipid and biomass enhancement st
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de Morais, Michele Greque, Bruna da Silva Vaz, Etiele Greque de Morais, and Jorge Alberto Vieira Costa. "Biologically Active Metabolites Synthesized by Microalgae." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/835761.

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Microalgae are microorganisms that have different morphological, physiological, and genetic traits that confer the ability to produce different biologically active metabolites. Microalgal biotechnology has become a subject of study for various fields, due to the varied bioproducts that can be obtained from these microorganisms. When microalgal cultivation processes are better understood, microalgae can become an environmentally friendly and economically viable source of compounds of interest, because production can be optimized in a controlled culture. The bioactive compounds derived from micr
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Khaw, Yam Sim, Fatimah Md Yusoff, Hui Teng Tan, et al. "Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review." Marine Drugs 20, no. 10 (2022): 592. http://dx.doi.org/10.3390/md20100592.

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Fucoxanthin is one of the light-harvesting pigments in brown microalgae, which is increasingly gaining attention due to its numerous health-promoting properties. Currently, the production of microalgal fucoxanthin is not yet feasible from an economic perspective. However, the cultivation of microalgae at favourable conditions holds great potential to increase the viability of this fucoxanthin source. Hence, this study aimed to review the fucoxanthin production of microalgae under different conditions systematically. A literature search was performed using the Web of Science, Scopus and PubMed
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Yu, Byung Sun, Young Joon Sung, Min Eui Hong, and Sang Jun Sim. "Improvement of Photoautotrophic Algal Biomass Production after Interrupted CO2 Supply by Urea and KH2PO4 Injection." Energies 14, no. 3 (2021): 778. http://dx.doi.org/10.3390/en14030778.

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Microalgae-derived biomass is currently considered a sustainable feedstock for making biofuels, including biodiesel and direct combustion fuel. The photoautotrophic cultivation of microalgae using flue gas from power plants has been continuously investigated to improve the economic feasibility of microalgae processes. The utilization of waste CO2 from power plants is advantageous in reducing carbon footprints and the cost of carbon sources. Nonetheless, the sudden interruption of CO2 supply during microalgal cultivation leads to a severe reduction in biomass productivity. Herein, chemical fert
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Fábregas, Jaime, Manuel Patiño, Ever D. Morales, Adolfo Dominguez, and Ana Otero. "Distinctive control of metabolic pathways byChlorella autotrophicain semicontinuous culture." Canadian Journal of Microbiology 42, no. 11 (1996): 1087–90. http://dx.doi.org/10.1139/m96-139.

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The marine microalga Chlorella autotrophica was cultured semicontinuously under light–dark synchronizing conditions at two nutrient concentrations (2 and 4 mmol N∙L−1) and five rates of daily renewal (from 10 to 50% of culture volume). Under such conditions, the biochemical composition of C. autotrophica was strongly influenced by the renewal rate, but unlike other marine microalgae, the nutrient concentration had no effect on the biochemical composition of the organic fraction of the microalga at a given growth rate. Results suggest that this species exerts a strong control over metabolic pat
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Moreira, Juliana Botelho, Suelen Goettems Kuntzler, Priscilla Quenia Muniz Bezerra, et al. "Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants." Polysaccharides 3, no. 1 (2022): 264–76. http://dx.doi.org/10.3390/polysaccharides3010015.

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Microalgae are used in flocculation processes because biopolymers are released into the culture medium. Microalgal cell growth under specific conditions (temperature, pH, luminosity, nutrients, and salinity) provides the production and release of exopolysaccharides (EPS). These biopolymers can be recovered from the medium for application as bioflocculants or used directly in cultivation as microalgae autoflocculants. The optimization of nutritional parameters, the control of process conditions, and the possibility of scaling up allow the production and industrial application of microalgal EPS.
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Cea-Barcia, Glenda, Gloria Moreno, and Germán Buitrón. "Anaerobic digestion of mixed microalgae cultivated in secondary effluent under mesophilic and thermophilic conditions." Water Science and Technology 72, no. 8 (2015): 1398–403. http://dx.doi.org/10.2166/wst.2015.344.

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The anaerobic digestion of mixed indigenous microalgae, grown in a secondary effluent, was evaluated in batch tests at mesophilic (35°C) and thermophilic (50°C) conditions. Under mesophilic conditions, specific methane production varied from 178 to 207 mL CH4/g volatile solids (VS) and the maximum production rate varied from 8.8 to 26.1 mL CH4/(gVS day), depending on the type of microalgae culture. Lower methane parameters were observed in those cultures where Scenedesmus represents more than 95% of the microalge. The culture with the lowest digestion performances under mesophilic conditions w
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Supono, Supono, Maria Mugica, Stefan Spreitzenbarth, and Andrew Jeffs. "Potential for Concentrated Microalgae as Replacement Diets for Juvenile Green-Lipped Mussels, Perna canaliculus." Aquaculture Research 2023 (February 21, 2023): 1–9. http://dx.doi.org/10.1155/2023/9841172.

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The nursery culture of bivalves typically relies on the feeding of costly live microalgae, while the use of natural sources of phytoplankton for feed is uncertain due to their variable quality and abundance. Replacement diets have been applied in bivalve nursery culture to replace live microalgae with varying success. This study investigated the potential use of two concentrated microalgal diets at a range of levels of substitution with live microalgae. Shellfish Diet 1800® (called SD) and LPB™ Frozen Shellfish Diet® (called LPB) were fed to juvenile green-lipped mussels (Perna canaliculus) at
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Yuan, Hao, Yi Wang, Yanaoming Xi, et al. "Light-Emitting Diode Power Conversion Capability and CO2 Fixation Rate of Microalgae Biofilm Cultured Under Different Light Spectra." Energies 13, no. 7 (2020): 1536. http://dx.doi.org/10.3390/en13071536.

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Microalgae biofilm-based culture has attracted much interest due to its high harvest efficiency and low energy requirements. Using light-emitting diodes (LEDs) as light source for microalgae culture has been considered as a promising choice to enhance the economic feasibility of microalgae-based commodities. In this work, the LED power conversion capability and CO2 fixation rate of microalgae biofilms (Chlorella ellipsoidea and Chlorella pyrenoidosa) cultured under different light spectra (white, blue, green and red) were studied. The results indicated that the power-to-biomass conversion capa
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Insan, Achmad Ilalqisny, Christiani Christiani, Hexa Apriliana Hidayah, and Dwi Sunu Widyartini. "The Lipid Content of The Culture Microalgae Using Media of Tapioca Liquid Waste." Biosaintifika: Journal of Biology & Biology Education 10, no. 2 (2018): 440–48. http://dx.doi.org/10.15294/biosaintifika.v10i2.12381.

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Microalga Navicula sp., Spirulina platensis, and Chlorella vulgaris have the prospect of being a source of biofuel producers. Rapid cell growth, coupled with the ability to produce large lipids and less pollution, can be used as an alternative to biofuel development. Microalgae cultivation can utilize tapioca liquid waste. Addition of NaCl to regulate salinity, so optimum for microalgae growth. In addition it can reduce toxins by binding to dissolved cyanide acid present in the waste. This study aims to determine the effect of NaCl concentration on tapioca liquid waste on growth and lipid micr
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Branyikova, Irena, Gita Prochazkova, Tomas Potocar, Zuzana Jezkova, and Tomas Branyik. "Harvesting of Microalgae by Flocculation." Fermentation 4, no. 4 (2018): 93. http://dx.doi.org/10.3390/fermentation4040093.

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Due to increasing demands for microalgal biomass and products originating from microalgae, large-scale production systems are necessary. However, current microalgal production technologies are not cost-effective and are hindered by various bottlenecks, one of which is the harvesting of microalgal biomass. Cell separation is difficult because of the low sedimentation velocity of microalgae, their colloidal character with repelling negative surface charges, and low biomass concentrations in culture broths; therefore, large volumes need to be processed in order to concentrate the cells. Flocculat
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