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Journal articles on the topic 'CUP1'

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Published: 4 June 2021
Last updated: 10 March 2023

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1

Knight, S. A., K. T. Tamai, D. J. Kosman, and D. J. Thiele. "Identification and analysis of a Saccharomyces cerevisiae copper homeostasis gene encoding a homeodomain protein." Molecular and Cellular Biology 14, no. 12 (1994): 7792–804. http://dx.doi.org/10.1128/mcb.14.12.7792-7804.1994.

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Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which permits yeast cells to grow at high concentrations of environmental copper, most notably when lactate is the sole carbon source. Disruption of CUP9, which is located on chromosome XVI, caused a loss of copper resistance in strains which possessed CUP1 and ACE1, as well as in the cu
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2

Knight, S. A., K. T. Tamai, D. J. Kosman, and D. J. Thiele. "Identification and analysis of a Saccharomyces cerevisiae copper homeostasis gene encoding a homeodomain protein." Molecular and Cellular Biology 14, no. 12 (1994): 7792–804. http://dx.doi.org/10.1128/mcb.14.12.7792.

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Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which permits yeast cells to grow at high concentrations of environmental copper, most notably when lactate is the sole carbon source. Disruption of CUP9, which is located on chromosome XVI, caused a loss of copper resistance in strains which possessed CUP1 and ACE1, as well as in the cu
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3

Wimalarathna, Roshini N., Po Yun Pan, and Chang-Hui Shen. "Co-dependent recruitment of Ino80p and Snf2p is required for yeast CUP1 activation." Biochemistry and Cell Biology 92, no. 1 (2014): 69–75. http://dx.doi.org/10.1139/bcb-2013-0097.

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In yeast, Ace1p-dependent induction of CUP1 is responsible for protecting cells from copper toxicity. Although the mechanism of yeast CUP1 induction has been studied intensively, it is still uncertain which chromatin remodelers are involved in CUP1 transcriptional activation. Here, we show that yeast cells are inviable in the presence of copper when either chromatin remodeler, Ino80p or Snf2p, is not present. This inviability is due to the lack of CUP1 expression in ino80Δ and snf2Δ cells. Subsequently, we observe that both Ino80p and Snf2p are present at the promoter and they are responsible
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4

Shen, Chang-Hui, Benoit P. Leblanc, Carolyn Neal, Ramin Akhavan, and David J. Clark. "Targeted Histone Acetylation at the Yeast CUP1 Promoter Requires the Transcriptional Activator, the TATA Boxes, and the Putative Histone Acetylase Encoded by SPT10." Molecular and Cellular Biology 22, no. 18 (2002): 6406–16. http://dx.doi.org/10.1128/mcb.22.18.6406-6416.2002.

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ABSTRACT The relationship between chromatin remodeling and histone acetylation at the yeast CUP1 gene was addressed. CUP1 encodes a metallothionein required for cell growth at high copper concentrations. Induction of CUP1 with copper resulted in targeted acetylation of both H3 and H4 at the CUP1 promoter. Nucleosomes containing upstream activating sequences and sequences farther upstream were the targets for H3 acetylation. Targeted acetylation of H3 and H4 required the transcriptional activator (Ace1p) and the TATA boxes, suggesting that targeted acetylation occurs when TATA-binding protein b
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5

Buchman, C., P. Skroch, W. Dixon, T. D. Tullius, and M. Karin. "A single amino acid change in CUP2 alters its mode of DNA binding." Molecular and Cellular Biology 10, no. 9 (1990): 4778–87. http://dx.doi.org/10.1128/mcb.10.9.4778-4787.1990.

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CUP2 is a copper-dependent transcriptional activator of the yeast CUP1 metallothionein gene. In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc). Using mobility shift, methylation interference, and DNase I and hydroxyl radical footprinting assays, we examined the interaction of wild-type and variant CUP2 proteins produced in Escherichia coli with the UASc. Our results suggest that CUP2 has a complex Cu-coordinated DNA-binding domain containing different part
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6

Buchman, C., P. Skroch, W. Dixon, T. D. Tullius, and M. Karin. "A single amino acid change in CUP2 alters its mode of DNA binding." Molecular and Cellular Biology 10, no. 9 (1990): 4778–87. http://dx.doi.org/10.1128/mcb.10.9.4778.

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CUP2 is a copper-dependent transcriptional activator of the yeast CUP1 metallothionein gene. In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc). Using mobility shift, methylation interference, and DNase I and hydroxyl radical footprinting assays, we examined the interaction of wild-type and variant CUP2 proteins produced in Escherichia coli with the UASc. Our results suggest that CUP2 has a complex Cu-coordinated DNA-binding domain containing different part
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7

Thiele, D. J. "ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene." Molecular and Cellular Biology 8, no. 7 (1988): 2745–52. http://dx.doi.org/10.1128/mcb.8.7.2745-2752.1988.

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Copper resistance in Saccharomyces cerevisiae is mediated, in large part, by the CUP1 locus, which encodes a low-molecular-weight, cysteine-rich metal-binding protein. Expression of the CUP1 gene is regulated at the level of transcriptional induction in response to high environmental copper levels. This report describes the isolation of a yeast mutant, ace1-1, which is defective in the activation of CUP1 expression upon exposure to exogenous copper. The ace1-1 mutation is recessive and lies in a genetic element that encodes a trans-acting CUP1 regulatory factor. The wild-type ACE1 gene was iso
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8

Silar, P., G. Butler, and D. J. Thiele. "Heat shock transcription factor activates transcription of the yeast metallothionein gene." Molecular and Cellular Biology 11, no. 3 (1991): 1232–38. http://dx.doi.org/10.1128/mcb.11.3.1232-1238.1991.

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In the yeast Saccharomyces cerevisiae, transcription of the metallothionein gene CUP1 is induced by copper and silver. Strains with a complete deletion of the ACE1 gene, the copper-dependent activator of CUP1 transcription, are hypersensitive to copper. These strains have a low but significant basal level of CUP1 transcription. To identify genes which mediate basal transcription of CUP1 or which activate CUP1 in response to other stimuli, we isolated an extragenic suppressor of an ace1 deletion. We demonstrate that a single amino acid substitution in the heat shock transcription factor (HSF) D
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9

Silar, P., G. Butler, and D. J. Thiele. "Heat shock transcription factor activates transcription of the yeast metallothionein gene." Molecular and Cellular Biology 11, no. 3 (1991): 1232–38. http://dx.doi.org/10.1128/mcb.11.3.1232.

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In the yeast Saccharomyces cerevisiae, transcription of the metallothionein gene CUP1 is induced by copper and silver. Strains with a complete deletion of the ACE1 gene, the copper-dependent activator of CUP1 transcription, are hypersensitive to copper. These strains have a low but significant basal level of CUP1 transcription. To identify genes which mediate basal transcription of CUP1 or which activate CUP1 in response to other stimuli, we isolated an extragenic suppressor of an ace1 deletion. We demonstrate that a single amino acid substitution in the heat shock transcription factor (HSF) D
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10

Thiele, D. J. "ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene." Molecular and Cellular Biology 8, no. 7 (1988): 2745–52. http://dx.doi.org/10.1128/mcb.8.7.2745.

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Copper resistance in Saccharomyces cerevisiae is mediated, in large part, by the CUP1 locus, which encodes a low-molecular-weight, cysteine-rich metal-binding protein. Expression of the CUP1 gene is regulated at the level of transcriptional induction in response to high environmental copper levels. This report describes the isolation of a yeast mutant, ace1-1, which is defective in the activation of CUP1 expression upon exposure to exogenous copper. The ace1-1 mutation is recessive and lies in a genetic element that encodes a trans-acting CUP1 regulatory factor. The wild-type ACE1 gene was iso
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11

Butler, G., and D. J. Thiele. "ACE2, an activator of yeast metallothionein expression which is homologous to SWI5." Molecular and Cellular Biology 11, no. 1 (1991): 476–85. http://dx.doi.org/10.1128/mcb.11.1.476-485.1991.

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Transcription of the Saccharomyces cerevisiae metallothionein gene CUP1 is induced in response to high environmental levels of copper. Induction requires the ACE1 gene product, which binds to specific sites in the promoter region of the CUP1 gene. In this study, we found that deleting the entire coding sequence of the ACE1 gene resulted in a decrease in basal-level transcription of CUP1 to low but detectable levels and conferred a copper-sensitive phenotype to the cells. We have isolated a gene, designated ACE2, which when present on a high-copy-number plasmid suppresses the copper-sensitive p
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12

Tamai, K. T., X. Liu, P. Silar, T. Sosinowski, and D. J. Thiele. "Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways." Molecular and Cellular Biology 14, no. 12 (1994): 8155–65. http://dx.doi.org/10.1128/mcb.14.12.8155-8165.1994.

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Metallothioneins constitute a class of low-molecular-weight, cysteine-rich metal-binding stress proteins which are biosynthetically regulated at the level of gene transcription in response to metals, hormones, cytokines, and other physiological and environmental stresses. In this report, we demonstrate that the Saccharomyces cerevisiae metallothionein gene, designated CUP1, is transcriptionally activated in response to heat shock and glucose starvation through the action of heat shock transcription factor (HSF) and a heat shock element located within the CUP1 promoter upstream regulatory regio
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13

Butler, G., and D. J. Thiele. "ACE2, an activator of yeast metallothionein expression which is homologous to SWI5." Molecular and Cellular Biology 11, no. 1 (1991): 476–85. http://dx.doi.org/10.1128/mcb.11.1.476.

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Transcription of the Saccharomyces cerevisiae metallothionein gene CUP1 is induced in response to high environmental levels of copper. Induction requires the ACE1 gene product, which binds to specific sites in the promoter region of the CUP1 gene. In this study, we found that deleting the entire coding sequence of the ACE1 gene resulted in a decrease in basal-level transcription of CUP1 to low but detectable levels and conferred a copper-sensitive phenotype to the cells. We have isolated a gene, designated ACE2, which when present on a high-copy-number plasmid suppresses the copper-sensitive p
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14

Tamai, K. T., X. Liu, P. Silar, T. Sosinowski, and D. J. Thiele. "Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways." Molecular and Cellular Biology 14, no. 12 (1994): 8155–65. http://dx.doi.org/10.1128/mcb.14.12.8155.

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Metallothioneins constitute a class of low-molecular-weight, cysteine-rich metal-binding stress proteins which are biosynthetically regulated at the level of gene transcription in response to metals, hormones, cytokines, and other physiological and environmental stresses. In this report, we demonstrate that the Saccharomyces cerevisiae metallothionein gene, designated CUP1, is transcriptionally activated in response to heat shock and glucose starvation through the action of heat shock transcription factor (HSF) and a heat shock element located within the CUP1 promoter upstream regulatory regio
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15

Buchman, C., P. Skroch, J. Welch, S. Fogel, and M. Karin. "The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein." Molecular and Cellular Biology 9, no. 9 (1989): 4091–95. http://dx.doi.org/10.1128/mcb.9.9.4091-4095.1989.

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CUP2 is a regulatory gene controlling expression of CUP1, which encodes the Cu-binding yeast metallothionein. CUP2, which is identical to the ACE1 gene, encodes a Cu-regulated DNA-binding protein. The CUP2 protein contains a cysteine-rich DNA-binding domain dependent on Cu+ and Ag+ ions which bind the cysteine residues and direct the refolding of the metal-free apoprotein. CUP2 mutant alleles from Cu-sensitive yeast strains have point mutations affecting the DNA-binding activity. These results establish CUP2 as the primary sensor of intracellular Cu+ in the yeast Saccharomyces cerevisiae, func
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16

Buchman, C., P. Skroch, J. Welch, S. Fogel, and M. Karin. "The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein." Molecular and Cellular Biology 9, no. 9 (1989): 4091–95. http://dx.doi.org/10.1128/mcb.9.9.4091.

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CUP2 is a regulatory gene controlling expression of CUP1, which encodes the Cu-binding yeast metallothionein. CUP2, which is identical to the ACE1 gene, encodes a Cu-regulated DNA-binding protein. The CUP2 protein contains a cysteine-rich DNA-binding domain dependent on Cu+ and Ag+ ions which bind the cysteine residues and direct the refolding of the metal-free apoprotein. CUP2 mutant alleles from Cu-sensitive yeast strains have point mutations affecting the DNA-binding activity. These results establish CUP2 as the primary sensor of intracellular Cu+ in the yeast Saccharomyces cerevisiae, func
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17

Whale, Alex J., Michelle King, Ryan M. Hull, Felix Krueger, and Jonathan Houseley. "Stimulation of adaptive gene amplification by origin firing under replication fork constraint." Nucleic Acids Research 50, no. 2 (2022): 915–36. http://dx.doi.org/10.1093/nar/gkab1257.

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Abstract Adaptive mutations can cause drug resistance in cancers and pathogens, and increase the tolerance of agricultural pests and diseases to chemical treatment. When and how adaptive mutations form is often hard to discern, but we have shown that adaptive copy number amplification of the copper resistance gene CUP1 occurs in response to environmental copper due to CUP1 transcriptional activation. Here we dissect the mechanism by which CUP1 transcription in budding yeast stimulates copy number variation (CNV). We show that transcriptionally stimulated CNV requires TREX-2 and Mediator, such
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18

Shen, Chang-Hui, Benoit P. Leblanc, Jennifer A. Alfieri, and David J. Clark. "Remodeling of Yeast CUP1 Chromatin Involves Activator-Dependent Repositioning of Nucleosomes over the Entire Gene and Flanking Sequences." Molecular and Cellular Biology 21, no. 2 (2001): 534–47. http://dx.doi.org/10.1128/mcb.21.2.534-547.2001.

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ABSTRACT The yeast CUP1 gene is activated by the copper-dependent binding of the transcriptional activator, Ace1p. An episome containing transcriptionally active or inactiveCUP1 was purified in its native chromatin structure from yeast cells. The amount of RNA polymerase II on CUP1 in the purified episomes correlated with its transcriptional activity in vivo. Chromatin structures were examined by using the monomer extension technique to map translational positions of nucleosomes. The chromatin structure of an episome containing inactive CUP1 isolated from ace1Δ cells is organized into clusters
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19

Thiele, D. J., and D. H. Hamer. "Tandemly duplicated upstream control sequences mediate copper-induced transcription of the Saccharomyces cerevisiae copper-metallothionein gene." Molecular and Cellular Biology 6, no. 4 (1986): 1158–63. http://dx.doi.org/10.1128/mcb.6.4.1158-1163.1986.

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Transcription of the Saccharomyces cerevisiae copper-metallothionein gene, CUP1, inducible by copper. By analyzing deletion and fusion mutants in the CUP1 5'-flanking region, we identified two closely related, tandemly arranged copper regulatory elements. A synthetic version of one of these elements conferred efficient copper induction on a heterologous promoter when present in two tandem copies.
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20

Thiele, D. J., and D. H. Hamer. "Tandemly duplicated upstream control sequences mediate copper-induced transcription of the Saccharomyces cerevisiae copper-metallothionein gene." Molecular and Cellular Biology 6, no. 4 (1986): 1158–63. http://dx.doi.org/10.1128/mcb.6.4.1158.

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Transcription of the Saccharomyces cerevisiae copper-metallothionein gene, CUP1, inducible by copper. By analyzing deletion and fusion mutants in the CUP1 5'-flanking region, we identified two closely related, tandemly arranged copper regulatory elements. A synthetic version of one of these elements conferred efficient copper induction on a heterologous promoter when present in two tandem copies.
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21

Lesser, C. F., and C. Guthrie. "Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1." Genetics 133, no. 4 (1993): 851–63. http://dx.doi.org/10.1093/genetics/133.4.851.

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Abstract We have developed a new reporter gene fusion to monitor mRNA splicing in yeast. An intron-containing fragment from the Saccharomyces cerevisiae ACT1 gene has been fused to CUP1, the yeast metallothionein homolog. CUP1 is a nonessential gene that allows cells to grow in the presence of copper in a dosage-dependent manner. By inserting previously characterized intron mutations into the fusion construct, we have established that the efficiency of splicing correlates with the level of copper resistance of these strains. A highly sensitive assay for 5' splice site usage was designed by eng
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22

Keil, R. L., and A. D. McWilliams. "A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae." Genetics 135, no. 3 (1993): 711–18. http://dx.doi.org/10.1093/genetics/135.3.711.

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Abstract The preservation of sequence homogeneity and copy number of tandemly repeated genes may require specific mechanisms or regulation of recombination. We have identified mutations that specifically affect recombination among natural repetitions in the yeast Saccharomyces cerevisiae. The rrm3 mutation stimulates mitotic recombination in the naturally occurring tandem repeats of the rDNA and copper chelatin (CUP1) genes. This mutation does not affect recombination of several other types of repeated genes tested including Ty elements, mating type information and duplications created by tran
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23

Yu, W., R. A. Farrell, D. J. Stillman, and D. R. Winge. "Identification of SLF1 as a new copper homeostasis gene involved in copper sulfide mineralization in Saccharomyces cerevisiae." Molecular and Cellular Biology 16, no. 5 (1996): 2464–72. http://dx.doi.org/10.1128/mcb.16.5.2464.

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In Saccharomyces cerevisiae, at least 12 genes are important for cells to propagate in medium containing elevated concentrations of copper salts (J. Welch, S. Fogel, C. Buchman, and M. Karin, EMBO J. 8:255-260, 1989). Complementation studies were carried out on a copper-sensitive mutation (cup14) from this group. A new yeast gene, designated SLF1, was identified as a multicopy suppressor of the cup14 mutation. Slf1 is important for the physiological process of copper sulfide (CuS) mineralization on the surface of cells cultured in medium containing copper salts. CuS mineralization causes the c
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24

Durrin, L. K., R. K. Mann, and M. Grunstein. "Nucleosome loss activates CUP1 and HIS3 promoters to fully induced levels in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 12, no. 4 (1992): 1621–29. http://dx.doi.org/10.1128/mcb.12.4.1621-1629.1992.

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We have previously shown that nucleosome loss, obtained by repressing histone H4 mRNA synthesis, activates otherwise inactive PHO5, GAL1, and CYC1 gene promoters (fused to the bacterial beta-galactosidase [lacZ] reporter gene) to moderate levels of activity (approximately 2 to 15% of fully induced levels). We now report that nucleosome loss activates the expression of two additional promoters that are normally induced by independent mechanisms: CUP1 (induced by heavy-metal toxicity) and HIS3 (induced by amino acid starvation). Surprisingly, the level of CUP1-lacZ and HIS3-lacZ activation by nu
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25

Durrin, L. K., R. K. Mann, and M. Grunstein. "Nucleosome loss activates CUP1 and HIS3 promoters to fully induced levels in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 12, no. 4 (1992): 1621–29. http://dx.doi.org/10.1128/mcb.12.4.1621.

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We have previously shown that nucleosome loss, obtained by repressing histone H4 mRNA synthesis, activates otherwise inactive PHO5, GAL1, and CYC1 gene promoters (fused to the bacterial beta-galactosidase [lacZ] reporter gene) to moderate levels of activity (approximately 2 to 15% of fully induced levels). We now report that nucleosome loss activates the expression of two additional promoters that are normally induced by independent mechanisms: CUP1 (induced by heavy-metal toxicity) and HIS3 (induced by amino acid starvation). Surprisingly, the level of CUP1-lacZ and HIS3-lacZ activation by nu
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26

Mortimer, Robert K., and John R. Johnston. "GENEALOGY OF PRINCIPAL STRAINS OF THE YEAST GENETIC STOCK CENTER." Genetics 113, no. 1 (1986): 35–43. http://dx.doi.org/10.1093/genetics/113.1.35.

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ABSTRACT We have constructed a genealogy of strain S288C, from which many of the mutant and segregant strains currently used in studies on the genetics and molecular biology of Saccharomyces cerevisiae have been derived. We have determined that its six progenitor strains were EM93, EM126, NRRL YB-210 and the three baking strains Yeast Foam, FLD and LK. We have estimated that approximately 88% of the gene pool of S288C is contributed by strain EM93. The principal ancestral genotypes were those of segregant strains EM93-1C and EM93-3B, initially distributed by C. C. Lindegren to several laborato
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27

Welch, J., S. Fogel, C. Buchman, and M. Karin. "The CUP2 gene product regulates the expression of the CUP1 gene, coding for yeast metallothionein." EMBO Journal 8, no. 1 (1989): 255–60. http://dx.doi.org/10.1002/j.1460-2075.1989.tb03371.x.

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28

Badi, L. "The CUP1 upstream repeated element renders CUP1 promoter activation insensitive to mutations in the RNA polymerase II transcription complex." Nucleic Acids Research 30, no. 6 (2002): 1306–15. http://dx.doi.org/10.1093/nar/30.6.1306.

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29

Peña, Maria Marjorette O., Keith A. Koch, and Dennis J. Thiele. "Dynamic Regulation of Copper Uptake and Detoxification Genes in Saccharomyces cerevisiae." Molecular and Cellular Biology 18, no. 5 (1998): 2514–23. http://dx.doi.org/10.1128/mcb.18.5.2514.

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ABSTRACT The essential yet toxic nature of copper demands tight regulation of the copper homeostatic machinery to ensure that sufficient copper is present in the cell to drive essential biochemical processes yet prevent the accumulation to toxic levels. In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genesCTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, andSOD1 in response to copper. In this study, we characterize
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30

Ruer, Ségolène, Silke Stender, Alain Filloux, and Sophie de Bentzmann. "Assembly of Fimbrial Structures in Pseudomonas aeruginosa: Functionality and Specificity of Chaperone-Usher Machineries." Journal of Bacteriology 189, no. 9 (2007): 3547–55. http://dx.doi.org/10.1128/jb.00093-07.

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ABSTRACT Fimbrial or nonfimbrial adhesins assembled by the bacterial chaperone-usher pathway have been demonstrated to play a key role in pathogenesis. Such an assembly mechanism has been exemplified in uropathogenic Escherichia coli strains with the Pap and the Fim systems. In Pseudomonas aeruginosa, three gene clusters (cupA, cupB, and cupC) encoding chaperone-usher pathway components have been identified in the genome sequence of the PAO1 strain. The Cup systems differ from the Pap or Fim systems, since they obviously lack numbers of genes encoding fimbrial subunits. Nevertheless, the CupA
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31

Xie, X. X., Y. F. Ma, Q. S. Wang, Z. L. Chen, R. R. Liao, and Y. C. Pan. "Yeast CUP1 protects HeLa cells against copper-induced stress." Brazilian Journal of Medical and Biological Research 48, no. 7 (2015): 616–21. http://dx.doi.org/10.1590/1414-431x20153848.

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32

Macreadie, Ian G., Ourania Horaitis, Paul R. Vaughan, and G. Des Clark-Walker. "Constitutive expression of theSaccharomyces cerevisiae CUP1 gene inKluyveromyces lactis." Yeast 7, no. 2 (1991): 127–35. http://dx.doi.org/10.1002/yea.320070206.

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33

Santoro, Nicholas, Nina Johansson, and Dennis J. Thiele. "Heat Shock Element Architecture Is an Important Determinant in the Temperature and Transactivation Domain Requirements for Heat Shock Transcription Factor." Molecular and Cellular Biology 18, no. 11 (1998): 6340–52. http://dx.doi.org/10.1128/mcb.18.11.6340.

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ABSTRACT The baker’s yeast Saccharomyces cerevisiae possesses a single gene encoding heat shock transcription factor (HSF), which is required for the activation of genes that participate in stress protection as well as normal growth and viability. Yeast HSF (yHSF) contains two distinct transcriptional activation regions located at the amino and carboxyl termini. Activation of the yeast metallothionein gene, CUP1, depends on a nonconsensus heat shock element (HSE), occurs at higher temperatures than other heat shock-responsive genes, and is highly dependent on the carboxyl-terminal transactivat
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34

Ozenberger, B. A., and G. S. Roeder. "A unique pathway of double-strand break repair operates in tandemly repeated genes." Molecular and Cellular Biology 11, no. 3 (1991): 1222–31. http://dx.doi.org/10.1128/mcb.11.3.1222-1231.1991.

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The RAD52 gene product of the yeast Saccharomyces cerevisiae is required for most spontaneous recombination and almost all double-strand break (DSB) repair. In contrast to recombination elsewhere in the genome, recombination in the ribosomal DNA (rDNA) array is RAD52 independent. To determine the fate of a DSB in the rDNA gene array, a cut site for the HO endonuclease was inserted into the rDNA in a strain containing an inducible HO gene. DSBs were efficiently repaired at this site, even in the absence of the RAD52 gene product. Efficient RAD52-independent DSB repair was also observed at anoth
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35

Ozenberger, B. A., and G. S. Roeder. "A unique pathway of double-strand break repair operates in tandemly repeated genes." Molecular and Cellular Biology 11, no. 3 (1991): 1222–31. http://dx.doi.org/10.1128/mcb.11.3.1222.

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The RAD52 gene product of the yeast Saccharomyces cerevisiae is required for most spontaneous recombination and almost all double-strand break (DSB) repair. In contrast to recombination elsewhere in the genome, recombination in the ribosomal DNA (rDNA) array is RAD52 independent. To determine the fate of a DSB in the rDNA gene array, a cut site for the HO endonuclease was inserted into the rDNA in a strain containing an inducible HO gene. DSBs were efficiently repaired at this site, even in the absence of the RAD52 gene product. Efficient RAD52-independent DSB repair was also observed at anoth
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36

Sousa, Carolina, Pavel Kotrba, Tomas Ruml, Angel Cebolla, and Víctor De Lorenzo. "Metalloadsorption by Escherichia coliCells Displaying Yeast and Mammalian Metallothioneins Anchored to the Outer Membrane Protein LamB." Journal of Bacteriology 180, no. 9 (1998): 2280–84. http://dx.doi.org/10.1128/jb.180.9.2280-2284.1998.

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ABSTRACT Yeast (CUP1) and mammalian (HMT-1A) metallothioneins (MTs) have been efficiently expressed in Escherichia coli as fusions to the outer membrane protein LamB. A 65-amino-acid sequence from the CUP1 protein of Saccharomyces cerevisiae (yeast [Y] MT) was genetically inserted in permissive site 153 of the LamB sequence, which faces the outer medium. A second LamB fusion at position 153 was created with 66 amino acids recruited from the form of human (H) MT that is predominant in the adipose tissue, HMT-1A. Both LamB153-YMT and LamB153-HMT hybrids were produced in vivo as full-length prote
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37

Evans, C. F., D. R. Engelke, and D. J. Thiele. "ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences." Molecular and Cellular Biology 10, no. 1 (1990): 426–29. http://dx.doi.org/10.1128/mcb.10.1.426-429.1990.

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The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.
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38

Evans, C. F., D. R. Engelke, and D. J. Thiele. "ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences." Molecular and Cellular Biology 10, no. 1 (1990): 426–29. http://dx.doi.org/10.1128/mcb.10.1.426.

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The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.
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39

Jeyaprakash, Ayyamperumal, Juliet W. Welch, and Seymour Fogel. "Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast." Molecular and General Genetics MGG 225, no. 3 (1991): 363–68. http://dx.doi.org/10.1007/bf00261675.

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40

Naumov, G. I., E. S. Naumova, N. N. Martynenko, and M. Korhola. "Reidentification of chromosomal CUP1 translocations in the wine yeasts Saccharomyces cerevisiae." Microbiology 82, no. 2 (2013): 201–9. http://dx.doi.org/10.1134/s0026261713010104.

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Xie, Xiaoxian, Yufang Ma, Zhenliang Chen, et al. "Transgenic Mice Expressing Yeast CUP1 Exhibit Increased Copper Utilization from Feeds." PLoS ONE 9, no. 9 (2014): e107810. http://dx.doi.org/10.1371/journal.pone.0107810.

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42

Williams, Kim E., and David R. Olsen. "Gelatin expression from an engineered Saccharomyces cerevisiae CUP1 promoter in Pichia pastoris." Yeast 38, no. 6 (2021): 382–87. http://dx.doi.org/10.1002/yea.3554.

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43

Vallet, Isabelle, Stephen P. Diggle, Rachael E. Stacey, et al. "Biofilm Formation in Pseudomonas aeruginosa: Fimbrial cup Gene Clusters Are Controlled by the Transcriptional Regulator MvaT." Journal of Bacteriology 186, no. 9 (2004): 2880–90. http://dx.doi.org/10.1128/jb.186.9.2880-2890.2004.

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ABSTRACT Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical devices. Various stages of biofilm formation by this organism are mediated by extracellular appendages, such as type IV pili and flagella. Recently, we identified three P. aeruginosa gene clusters that were termed cup (chaperone-usher pathway) based on their sequence rel
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Welch, J. W., D. H. Maloney, and S. Fogel. "Gene conversions within the Cup1 r region from heterologous crosses in Saccharomyces cerevisiae." Molecular and General Genetics MGG 229, no. 2 (1991): 261–66. http://dx.doi.org/10.1007/bf00272164.

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45

Dorsey, Michael J., Paula Hoeh, and Charlotte E. Paquin. "Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae." Current Genetics 23, no. 5-6 (1993): 392–96. http://dx.doi.org/10.1007/bf00312624.

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46

Valoroso, Maria Carmen, Francesca Lucibelli, and Serena Aceto. "Orchid NAC Transcription Factors: A Focused Analysis of CUPULIFORMIS Genes." Genes 13, no. 12 (2022): 2293. http://dx.doi.org/10.3390/genes13122293.

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Plant transcription factors are involved in different developmental pathways. NAC transcription factors (No Apical Meristem, Arabidopsis thaliana Activating Factor, Cup-shaped Cotyledon) act in various processes, e.g., plant organ formation, response to stress, and defense mechanisms. In Antirrhinum majus, the NAC transcription factor CUPULIFORMIS (CUP) plays a role in determining organ boundaries and lip formation, and the CUP homologs of Arabidopsis and Petunia are involved in flower organ formation. Orchidaceae is one of the most species-rich families of angiosperms, known for its extraordi
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Steinle, Anna, Fred Bernd Oppermann-Sanio, Rudolf Reichelt, and Alexander Steinbüchel. "Synthesis and Accumulation of Cyanophycin in Transgenic Strains of Saccharomyces cerevisiae." Applied and Environmental Microbiology 74, no. 11 (2008): 3410–18. http://dx.doi.org/10.1128/aem.00366-08.

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ABSTRACT Cyanophycin [multi-l-arginyl-poly(l-aspartic acid) (CGP)] was, for the first time, produced in yeast. As yeasts are very important production organisms in biotechnology, it was determined if CGP can be produced in two different strains of Saccharomyces cerevisiae. The episomal vector systems pESC (with the galactose-inducible promoter GAL1) and pYEX-BX (with the copper ion-inducible promoter CUP1) were chosen to express the cyanophycin synthetase gene from the cyanobacterium Synechocystis sp. strain PCC 6308 (cphA 6308) in yeast. Expression experiments with transgenic yeasts revealed
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Mehta, Gunjan D., David Ball, and Tatiana Karpova. "Quantifying Transcription Dynamics of CUP1 gene of S. cerevisiae at the Single‐Molecule Level." FASEB Journal 34, S1 (2020): 1. http://dx.doi.org/10.1096/fasebj.2020.34.s1.09092.

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Kommuguri, Upendra Nadh, Sreedhar Bodiga, Suneetha Sankuru, and Vijaya Lakshmi Bodiga. "Copper deprivation modulates CTR1 and CUP1 expression and enhances cisplatin cytotoxicity in Saccharomyces cerevisiae." Journal of Trace Elements in Medicine and Biology 26, no. 1 (2012): 13–19. http://dx.doi.org/10.1016/j.jtemb.2011.12.001.

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Mascorro-Gallardo, J. O., A. A. Covarrubias, and R. Gaxiola. "Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae." Gene 172, no. 1 (1996): 169–70. http://dx.doi.org/10.1016/0378-1119(96)00059-5.

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