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Journal articles on the topic 'Cupriavidus metallidurans CH34'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 February 2022

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1

AVOSCAN, L., H. KHODJA, M. CARRIÈRE, J. COVÈS, and B. GOUGET. "PIXE ANALYSES OF THE SOLUBLE AND MEMBRANE SE-CONTAINING PROTEINS EXTRACTED FROMCUPRIAVIDUS METALLIDURANSCH34 AFTER SELENIUM OXIDES CHALLENGE." International Journal of PIXE 18, no. 03n04 (2008): 91–99. http://dx.doi.org/10.1142/s0129083508001430.

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The soil bacterium Cupriavidus metallidurans CH34 resist selenite by reducing it into the insoluble and less toxic elemental selenium. Two mechanisms of reduction of selenium oxides in C. metallidurans CH34 were highlighted: assimilation leading to organic species and detoxification leading to precipitation of selenite in nanoparticules of elemental selenium. The alkyl selenide detected as an intermediate product during assimilation of selenite or as the major accumulated chemical form during assimilation of selenate was identified as selenomethionine.Soluble and membrane proteins were extracted from C. metallidurans CH34 submitted to selenium oxides challenge. After separation by SDS-PAGE, µPIXE analyses were used for Se identification and quantification at a micrometer scale. The profiles of Se distribution in the different samples suggest a non-specific incorporation of selenium probably reflecting the incorporation of selenomethionin in place of the naturally occurring methionin.

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2

Avoscan, Laure, Guillaume Untereiner, Jeril Degrouard, Marie Carriere, and Barbara Gouget. "Uranium and selenium resistance in Cupriavidus Metallidurans CH34." Toxicology Letters 172 (October 2007): S157. http://dx.doi.org/10.1016/j.toxlet.2007.05.403.

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3

Avoscan, Laure, Richard Collins, Marie Carriere, Barbara Gouget, and Jacques Covï¿½s. "Seleno-l-Methionine Is the Predominant Organic Form of Selenium in Cupriavidus metallidurans CH34 Exposed to Selenite or Selenate." Applied and Environmental Microbiology 72, no. 9 (2006): 6414–16. http://dx.doi.org/10.1128/aem.01084-06.

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ABSTRACT The accumulated organic form of selenium previously detected by X-ray absorption near-edge structure (XANES) analyses in Cupriavidus metallidurans CH34 exposed to selenite or selenate was identified as seleno-l-methionine by coupling high-performance liquid chromatography to inductively coupled plasma-mass spectrometry.

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4

Zhang, Yian-Biao, Sébastien Monchy, Bill Greenberg, et al. "ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34." Antonie van Leeuwenhoek 96, no. 2 (2009): 161–70. http://dx.doi.org/10.1007/s10482-009-9313-z.

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5

Rosier, Caroline, Natalie Leys, Céline Henoumont, Max Mergeay, and Ruddy Wattiez. "Purification and Characterization of the Acetone Carboxylase of Cupriavidus metallidurans Strain CH34." Applied and Environmental Microbiology 78, no. 12 (2012): 4516–18. http://dx.doi.org/10.1128/aem.07974-11.

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ABSTRACTAcetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for theHelicobacteraceaefamily, genome analyses revealed that bacteria that possess an Acx, such asCupriavidus metalliduransstrain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α2β2γ2and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

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6

Avoscan, Laure, Marie Carrière, Olivier Proux, et al. "Enhanced Selenate Accumulation in Cupriavidus metallidurans CH34 Does Not Trigger a Detoxification Pathway." Applied and Environmental Microbiology 75, no. 7 (2009): 2250–52. http://dx.doi.org/10.1128/aem.02452-08.

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ABSTRACT Cupriavidus metallidurans CH34 cells grown under sulfate-limited conditions accumulated up to six times more selenate than cells grown in sulfate-rich medium. The products of selenate reduction detected by X-ray absorption spectroscopy, electron microscopy, and energy-dispersive X-ray analysis did not define this strain as being a good candidate for bioremediation of selenate-contaminated environments.

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7

Millacura, Felipe, Paul Janssen, Pieter Monsieurs, et al. "Unintentional Genomic Changes Endow Cupriavidus metallidurans with an Augmented Heavy-Metal Resistance." Genes 9, no. 11 (2018): 551. http://dx.doi.org/10.3390/genes9110551.

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For the past three decades, Cupriavidus metallidurans has been one of the major model organisms for bacterial tolerance to heavy metals. Its type strain CH34 contains at least 24 gene clusters distributed over four replicons, allowing for intricate and multilayered metal responses. To gain organic mercury resistance in CH34, broad-spectrum mer genes were introduced in a previous work via conjugation of the IncP-1β plasmid pTP6. However, we recently noted that this CH34-derived strain, MSR33, unexpectedly showed an increased resistance to other metals (i.e., Co2+, Ni2+, and Cd2+). To thoroughly investigate this phenomenon, we resequenced the entire genome of MSR33 and compared its DNA sequence and basal gene expression profile to those of its parental strain CH34. Genome comparison identified 11 insertions or deletions (INDELs) and nine single nucleotide polymorphisms (SNPs), whereas transcriptomic analysis displayed 107 differentially expressed genes. Sequence data implicated the transposition of IS1088 in higher Co2+ and Ni2+ resistances and altered gene expression, although the precise mechanisms of the augmented Cd2+ resistance in MSR33 remains elusive. Our work indicates that conjugation procedures involving large complex genomes and extensive mobilomes may pose a considerable risk toward the introduction of unwanted, undocumented genetic changes. Special efforts are needed for the applied use and further development of small nonconjugative broad-host plasmid vectors, ideally involving CRISPR-related and advanced biosynthetic technologies.

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8

Maertens, Laurens, Natalie Leys, Jean-Yves Matroule, and Rob Van Houdt. "The Transcriptomic Landscape of Cupriavidus metallidurans CH34 Acutely Exposed to Copper." Genes 11, no. 9 (2020): 1049. http://dx.doi.org/10.3390/genes11091049.

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Bacteria are increasingly used for biotechnological applications such as bioremediation, biorecovery, bioproduction, and biosensing. The development of strains suited for such applications requires a thorough understanding of their behavior, with a key role for their transcriptomic landscape. We present a thorough analysis of the transcriptome of Cupriavidus metallidurans CH34 cells acutely exposed to copper by tagRNA-sequencing. C. metallidurans CH34 is a model organism for metal resistance, and its potential as a biosensor and candidate for metal bioremediation has been demonstrated in multiple studies. Several metabolic pathways were impacted by Cu exposure, and a broad spectrum of metal resistance mechanisms, not limited to copper-specific clusters, was overexpressed. In addition, several gene clusters involved in the oxidative stress response and the cysteine-sulfur metabolism were induced. In total, 7500 transcription start sites (TSSs) were annotated and classified with respect to their location relative to coding sequences (CDSs). Predicted TSSs were used to re-annotate 182 CDSs. The TSSs of 2422 CDSs were detected, and consensus promotor logos were derived. Interestingly, many leaderless messenger RNAs (mRNAs) were found. In addition, many mRNAs were transcribed from multiple alternative TSSs. We observed pervasive intragenic TSSs both in sense and antisense to CDSs. Antisense transcripts were enriched near the 5′ end of mRNAs, indicating a functional role in post-transcriptional regulation. In total, 578 TSSs were detected in intergenic regions, of which 35 were identified as putative small regulatory RNAs. Finally, we provide a detailed analysis of the main copper resistance clusters in CH34, which include many intragenic and antisense transcripts. These results clearly highlight the ubiquity of noncoding transcripts in the CH34 transcriptome, many of which are putatively involved in the regulation of metal resistance.

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9

Clavero-León, Claudia, Daniela Ruiz, Javier Cillero, Julieta Orlando, and Bernardo González. "The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper." PeerJ 9 (May 14, 2021): e11373. http://dx.doi.org/10.7717/peerj.11373.

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Copper (Cu) is important for plant growth, but high concentrations can lead to detrimental effects such as primary root length inhibition, vegetative tissue chlorosis, and even plant death. The interaction between plant-soil microbiota and roots can potentially affect metal mobility and availability, and, therefore, overall plant metal concentration. Cupriavidus metallidurans CH34 is a multi metal-resistant bacterial model that alters metal mobility and bioavailability through ion pumping, metal complexation, and reduction processes. The interactions between strain CH34 and plants may affect the growth, metal uptake, and translocation of Arabidopsis thaliana plants that are exposed to or not exposed to Cu. In this study, we looked also at the specific gene expression changes in C. metallidurans when co-cultured with Cu-exposed A. thaliana. We found that A. thaliana’s rosette area, primary and secondary root growth, and dry weight were affected by strain CH34, and that beneficial or detrimental effects depended on Cu concentration. An increase in some plant growth parameters was observed at copper concentrations lower than 50 µM and significant detrimental effects were found at concentrations higher than 50 µM Cu. We also observed up to a 90% increase and 60% decrease in metal accumulation and mobilization in inoculated A. thaliana. In turn, copper-stressed A. thaliana altered C. metallidurans colonization, and cop genes that encoded copper resistance in strain CH34 were induced by the combination of A. thaliana and Cu. These results reveal the complexity of the plant-bacteria-metal triad and will contribute to our understanding of their applications in plant growth promotion, protection, and phytoremediation strategies.

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10

Montero‐Silva, Francisco, Nelson Durán, and Michael Seeger. "Synthesis of extracellular gold nanoparticles using Cupriavidus metallidurans CH34 cells." IET Nanobiotechnology 12, no. 1 (2017): 40–46. http://dx.doi.org/10.1049/iet-nbt.2017.0185.

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11

Maillard, Antoine P., Sandra Künnemann, Cornelia Große, et al. "Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding." Metallomics 7, no. 4 (2015): 622–31. http://dx.doi.org/10.1039/c4mt00293h.

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12

Julian, Daniel J., Christopher J. Kershaw, Nigel L. Brown, and Jon L. Hobman. "Transcriptional activation of MerR family promoters in Cupriavidus metallidurans CH34." Antonie van Leeuwenhoek 96, no. 2 (2008): 149–59. http://dx.doi.org/10.1007/s10482-008-9293-4.

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13

Zoropogui, Anthony, Serge Gambarelli, and Jacques Covès. "CzcE from Cupriavidus metallidurans CH34 is a copper-binding protein." Biochemical and Biophysical Research Communications 365, no. 4 (2008): 735–39. http://dx.doi.org/10.1016/j.bbrc.2007.11.030.

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14

Kumar, Ranjai, Shadil Ibrahim Wani, Nar Singh Chauhan, Rakesh Sharma, and Dipti Sareen. "Cloning and characterization of an epoxide hydrolase from Cupriavidus metallidurans-CH34." Protein Expression and Purification 79, no. 1 (2011): 49–59. http://dx.doi.org/10.1016/j.pep.2011.04.007.

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15

Van Houdt, Rob, Joachim Vandecraen, Natalie Leys, Pieter Monsieurs, and Abram Aertsen. "Adaptation of Cupriavidus metallidurans CH34 to Toxic Zinc Concentrations Involves an Uncharacterized ABC-Type Transporter." Microorganisms 9, no. 2 (2021): 309. http://dx.doi.org/10.3390/microorganisms9020309.

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Cupriavidus metallidurans CH34 is a well-studied metal-resistant β-proteobacterium and contains a battery of genes participating in metal metabolism and resistance. Here, we generated a mutant (CH34ZnR) adapted to high zinc concentrations in order to study how CH34 could adaptively further increase its resistance against this metal. Characterization of CH34ZnR revealed that it was also more resistant to cadmium, and that it incurred seven insertion sequence-mediated mutations. Among these, an IS1088 disruption of the glpR gene (encoding a DeoR-type transcriptional repressor) resulted in the constitutive expression of the neighboring ATP-binding cassette (ABC)-type transporter. GlpR and the adjacent ABC transporter are highly similar to the glycerol operon regulator and ATP-driven glycerol importer of Rhizobium leguminosarum bv. viciae VF39, respectively. Deletion of glpR or the ABC transporter and complementation of CH34ZnR with the parental glpR gene further demonstrated that loss of GlpR function and concomitant derepression of the adjacent ABC transporter is pivotal for the observed resistance phenotype. Importantly, addition of glycerol, presumably by glycerol-mediated attenuation of GlpR activity, also promoted increased zinc and cadmium resistance in the parental CH34 strain. Upregulation of this ABC-type transporter is therefore proposed as a new adaptation route towards metal resistance.

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16

Mijnendonckx, Kristel, Ann Provoost, Pieter Monsieurs, et al. "Insertion sequence elements in Cupriavidus metallidurans CH34: Distribution and role in adaptation." Plasmid 65, no. 3 (2011): 193–203. http://dx.doi.org/10.1016/j.plasmid.2010.12.006.

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17

Zammit, Carla M., Florian Weiland, Joël Brugger, et al. "Proteomic responses to gold(iii)-toxicity in the bacterium Cupriavidus metallidurans CH34." Metallomics 8, no. 11 (2016): 1204–16. http://dx.doi.org/10.1039/c6mt00142d.

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18

Giagnoni, Laura, Francesca Magherini, Loretta Landi, et al. "Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34." Biology and Fertility of Soils 48, no. 4 (2011): 425–33. http://dx.doi.org/10.1007/s00374-011-0641-6.

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19

Van Houdt, Rob, Ann Provoost, Ado Van Assche, et al. "Cupriavidus metallidurans Strains with Different Mobilomes and from Distinct Environments Have Comparable Phenomes." Genes 9, no. 10 (2018): 507. http://dx.doi.org/10.3390/genes9100507.

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Cupriavidus metallidurans has been mostly studied because of its resistance to numerous heavy metals and is increasingly being recovered from other environments not typified by metal contamination. They host a large and diverse mobile gene pool, next to their native megaplasmids. Here, we used comparative genomics and global metabolic comparison to assess the impact of the mobilome on growth capabilities, nutrient utilization, and sensitivity to chemicals of type strain CH34 and three isolates (NA1, NA4 and H1130). The latter were isolated from water sources aboard the International Space Station (NA1 and NA4) and from an invasive human infection (H1130). The mobilome was expanded as prophages were predicted in NA4 and H1130, and a genomic island putatively involved in abietane diterpenoids metabolism was identified in H1130. An active CRISPR-Cas system was identified in strain NA4, providing immunity to a plasmid that integrated in CH34 and NA1. No correlation between the mobilome and isolation environment was found. In addition, our comparison indicated that the metal resistance determinants and properties are conserved among these strains and thus maintained in these environments. Furthermore, all strains were highly resistant to a wide variety of chemicals, much broader than metals. Only minor differences were observed in the phenomes (measured by phenotype microarrays), despite the large difference in mobilomes and the variable (shared by two or three strains) and strain-specific genomes.

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20

Ali, Md Muntasir, Ann Provoost, Laurens Maertens, et al. "Genomic and Transcriptomic Changes that Mediate Increased Platinum Resistance in Cupriavidus metallidurans." Genes 10, no. 1 (2019): 63. http://dx.doi.org/10.3390/genes10010063.

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The extensive anthropogenic use of platinum, a rare element found in low natural abundance in the Earth’s continental crust and one of the critical raw materials in the EU innovation partnership framework, has resulted in increased concentrations in surface environments. To minimize its spread and increase its recovery from the environment, biological recovery via different microbial systems is explored. In contrast, studies focusing on the effects of prolonged exposure to Pt are limited. In this study, we used the metal-resistant Cupriavidus metallidurans NA4 strain to explore the adaptation of environmental bacteria to platinum exposure. We used a combined Nanopore–Illumina sequencing approach to fully resolve all six replicons of the C. metallidurans NA4 genome, and compared them with the C. metallidurans CH34 genome, revealing an important role in metal resistance for its chromid rather than its megaplasmids. In addition, we identified the genomic and transcriptomic changes in a laboratory-evolved strain, displaying resistance to 160 µM Pt4+. The latter carried 20 mutations, including a large 69.9 kb deletion in its plasmid pNA4_D (89.6 kb in size), and 226 differentially-expressed genes compared to its parental strain. Many membrane-related processes were affected, including up-regulation of cytochrome c and a lytic transglycosylase, down-regulation of flagellar and pili-related genes, and loss of the pNA4_D conjugative machinery, pointing towards a significant role in the adaptation to platinum.

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Jarosławiecka, Anna, and Zofia Piotrowska-Seget. "Lead resistance in micro-organisms." Microbiology 160, no. 1 (2014): 12–25. http://dx.doi.org/10.1099/mic.0.070284-0.

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Lead (Pb) is an element present in the environment that negatively affects all living organisms. To diminish its high toxicity, micro-organisms have developed several mechanisms that allow them to survive exposure to Pb(II). The main mechanisms of lead resistance involve adsorption by extracellular polysaccharides, cell exclusion, sequestration as insoluble phosphates, and ion efflux to the cell exterior. This review describes the various lead resistance mechanisms, and the regulation of their expression by lead binding regulatory proteins. Special attention is given to the Pbr system from Cupriavidus metallidurans CH34, which involves a unique mechanism combining efflux and lead precipitation.

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22

Wiesemann, N., J. Mohr, C. Grosse, et al. "Influence of Copper Resistance Determinants on Gold Transformation by Cupriavidus metallidurans Strain CH34." Journal of Bacteriology 195, no. 10 (2013): 2298–308. http://dx.doi.org/10.1128/jb.01951-12.

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23

Pompidor, Guillaume, Eric Girard, Antoine Maillard, et al. "Biostructural analysis of the metal-sensor domain of CnrX from Cupriavidus metallidurans CH34." Antonie van Leeuwenhoek 96, no. 2 (2008): 141–48. http://dx.doi.org/10.1007/s10482-008-9283-6.

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24

Leys, Natalie, Sarah Baatout, Caroline Rosier, et al. "The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station." Antonie van Leeuwenhoek 96, no. 2 (2009): 227–45. http://dx.doi.org/10.1007/s10482-009-9360-5.

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25

Shamim, Saba, Abdul Rehman, and Mahmood Hussain Qazi. "Cadmium-Resistance Mechanism in the Bacteria Cupriavidus metallidurans CH34 and Pseudomonas putida mt2." Archives of Environmental Contamination and Toxicology 67, no. 2 (2014): 149–57. http://dx.doi.org/10.1007/s00244-014-0009-7.

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26

Ledrich, Marie-Laure, Sébastien Stemmler, Philippe Laval-Gilly, Laurent Foucaud, and Jaïro Falla. "Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34." BioMetals 18, no. 6 (2005): 643–50. http://dx.doi.org/10.1007/s10534-005-3858-8.

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27

Nies, Dietrich H., Grit Rehbein, Toni Hoffmann, Cindy Baumann, and Cornelia Grosse. "Paralogs of Genes Encoding Metal Resistance Proteins in Cupriavidus metallidurans Strain CH34." Journal of Molecular Microbiology and Biotechnology 11, no. 1-2 (2006): 82–93. http://dx.doi.org/10.1159/000092820.

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28

Kirsten, A., M. Herzberg, A. Voigt, et al. "Contributions of Five Secondary Metal Uptake Systems to Metal Homeostasis of Cupriavidus metallidurans CH34." Journal of Bacteriology 193, no. 18 (2011): 4652–63. http://dx.doi.org/10.1128/jb.05293-11.

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29

Llorens, Isabelle, Guillaume Untereiner, Danielle Jaillard, Barbara Gouget, Virginie Chapon, and Marie Carriere. "Uranium Interaction with Two Multi-Resistant Environmental Bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris." PLoS ONE 7, no. 12 (2012): e51783. http://dx.doi.org/10.1371/journal.pone.0051783.

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Taghavi, Safiyh, Celine Lesaulnier, Sebastien Monchy, Ruddy Wattiez, Max Mergeay, and Daniel van der Lelie. "Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions." Antonie van Leeuwenhoek 96, no. 2 (2008): 171–82. http://dx.doi.org/10.1007/s10482-008-9289-0.

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31

Silver, Simon, and Max Mergeay. "Introduction to a special Festschrift issue celebrating the microbiology of Cupriavidus metallidurans strain CH34." Antonie van Leeuwenhoek 96, no. 2 (2009): 113–14. http://dx.doi.org/10.1007/s10482-009-9357-0.

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32

Monsieurs, Pieter, Hugo Moors, Rob Van Houdt, et al. "Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network." BioMetals 24, no. 6 (2011): 1133–51. http://dx.doi.org/10.1007/s10534-011-9473-y.

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Rivas-Castillo, A. M., T. L. Monges-Rojas, and N. G. Rojas-Avelizapa. "Specificity of Mo and V Removal from a Spent Catalyst by Cupriavidus metallidurans CH34." Waste and Biomass Valorization 10, no. 4 (2017): 1037–42. http://dx.doi.org/10.1007/s12649-017-0093-9.

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Monchy, Sébastien, Mohammed A. Benotmane, Ruddy Wattiez, et al. "Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34." Microbiology 152, no. 6 (2006): 1765–76. http://dx.doi.org/10.1099/mic.0.28593-0.

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The four replicons of Cupriavidus metallidurans CH34 (the genome sequence was provided by the US Department of Energy–University of California Joint Genome Institute) contain two gene clusters putatively encoding periplasmic resistance to copper, with an arrangement of genes resembling that of the copSRABCD locus on the 2.1 Mb megaplasmid (MPL) of Ralstonia solanacearum, a closely related plant pathogen. One of the copSRABCD clusters was located on the 2.6 Mb MPL, while the second was found on the pMOL30 (234 kb) plasmid as part of a larger group of genes involved in copper resistance, spanning 17 857 bp in total. In this region, 19 ORFs (copVTMKNSRABCDIJGFLQHE) were identified based on the sequencing of a fragment cloned in an IncW vector, on the preliminary annotation by the Joint Genome Institute, and by using transcriptomic and proteomic data. When introduced into plasmid-cured derivatives of C. metallidurans CH34, the cop locus was able to restore the wild-type MIC, albeit with a biphasic survival curve, with respect to applied Cu(II) concentration. Quantitative-PCR data showed that the 19 ORFs were induced from 2- to 1159-fold when cells were challenged with elevated Cu(II) concentrations. Microarray data showed that the genes that were most induced after a Cu(II) challenge of 0.1 mM belonged to the pMOL30 cop cluster. Megaplasmidic cop genes were also induced, but at a much lower level, with the exception of the highly expressed MPL copD. Proteomic data allowed direct observation on two-dimensional gel electrophoresis, and via mass spectrometry, of pMOL30 CopK, CopR, CopS, CopA, CopB and CopC proteins. Individual cop gene expression depended on both the Cu(II) concentration and the exposure time, suggesting a sequential scheme in the resistance process, involving genes such as copK and copT in an initial phase, while other genes, such as copH, seem to be involved in a late response phase. A concentration of 0.4 mM Cu(II) was the highest to induce maximal expression of most cop genes.

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Hajdu, Rita, and Vera I. Slaveykova. "Cd and Pb removal from contaminated environment by metal resistant bacterium Cupriavidus metallidurans CH34: importance of the complexation and competition effects." Environmental Chemistry 9, no. 4 (2012): 389. http://dx.doi.org/10.1071/en12015.

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Environmental contextLive bacteria are widely used to remove toxic metals from contaminated environments. We use the metal-resistant bacterium Cupriavidus metallidurans, in both model solutions and aqueous extracts of soils, to investigate the complexation and competition effects on Cd and Pb uptake. Accumulation of Cd was more affected by competition with Ca, Mg and Zn, whereas Pb accumulation was more influenced by complexation with humic acids. The study highlights the need to consider chemical site-specificity in the removal of metals from contaminated environments. AbstractThe present study aims to improve the understanding of the role of complexation and competition effects on Cd and Pb accumulation by the metal resistant bacterium Cupriavidus metallidurans largely used in bioremediation. Adsorbed and intracellular metal content in bacteria were determined in model exposure medium within a concentration range spanning from 10–9 to 5 × 10–5 M of Cd or Pb and water extracts from soils. In parallel, the free metal ion concentrations ([M2+]) were measured by an ion exchange technique. Obtained results demonstrated that Cd and Pb accumulation by C. metallidurans was related to [M2+] in the solution. The adsorbed and intracellular M fractions were significantly reduced by nitrilotriacetic acid, Elliot or Pahokee Peat humic acids, as well as by a large excess of Ca, Mg and Zn. No effect on Cd and Pb bioaccumulation was observed in the presence of Mn, Cu or Co at a 10-fold excess for bacteria exposed to 10–6 M of Cd or Pb. Adsorbed and intracellular metal determined when bacteria were exposed to water extracts of soil were in the same order as expected from the model experiments when complexation and competition effects are considered. The study emphasises the necessity of taking into account chemical site-specificity of soil solutions and water, including dissolved organic ligands, pH and the presence of other metals when developing metal removal technologies by living bacteria.

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De Gelder, Joke, Peter Vandenabeele, Patrick De Boever, Max Mergeay, Luc Moens, and Paul De Vos. "Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions." Microgravity Science and Technology 21, no. 3 (2008): 217–23. http://dx.doi.org/10.1007/s12217-008-9037-0.

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37

Herzberg, M., L. Bauer, and D. H. Nies. "Deletion of the zupT gene for a zinc importer influences zinc pools in Cupriavidus metallidurans CH34." Metallomics 6, no. 3 (2014): 421. http://dx.doi.org/10.1039/c3mt00267e.

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38

Mikolay, André, and Dietrich H. Nies. "The ABC-transporter AtmA is involved in nickel and cobalt resistance of Cupriavidus metallidurans strain CH34." Antonie van Leeuwenhoek 96, no. 2 (2009): 183–91. http://dx.doi.org/10.1007/s10482-008-9303-6.

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Van Houdt, Rob, Sébastien Monchy, Natalie Leys, and Max Mergeay. "New mobile genetic elements in Cupriavidus metallidurans CH34, their possible roles and occurrence in other bacteria." Antonie van Leeuwenhoek 96, no. 2 (2009): 205–26. http://dx.doi.org/10.1007/s10482-009-9345-4.

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Grosse, Cornelia, Susann Friedrich, and Dietrich H. Nies. "Contribution of Extracytoplasmic Function Sigma Factors to Transition Metal Homeostasis in Cupriavidus metallidurans Strain CH34." Journal of Molecular Microbiology and Biotechnology 12, no. 3-4 (2007): 227–40. http://dx.doi.org/10.1159/000099644.

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Janssen, Paul J., Rob Van Houdt, Hugo Moors, et al. "The Complete Genome Sequence of Cupriavidus metallidurans Strain CH34, a Master Survivalist in Harsh and Anthropogenic Environments." PLoS ONE 5, no. 5 (2010): e10433. http://dx.doi.org/10.1371/journal.pone.0010433.

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Monchy, Sébastien, Mohammed A. Benotmane, Paul Janssen, et al. "Plasmids pMOL28 and pMOL30 of Cupriavidus metallidurans Are Specialized in the Maximal Viable Response to Heavy Metals." Journal of Bacteriology 189, no. 20 (2007): 7417–25. http://dx.doi.org/10.1128/jb.00375-07.

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Abstract:

ABSTRACT We fully annotated two large plasmids, pMOL28 (164 open reading frames [ORFs]; 171,459 bp) and pMOL30 (247 ORFs; 233,720 bp), in the genome of Cupriavidus metallidurans CH34. pMOL28 contains a backbone of maintenance and transfer genes resembling those found in plasmid pSym of C. taiwanensis and plasmid pHG1 of C. eutrophus, suggesting that they belong to a new class of plasmids. Genes involved in resistance to the heavy metals Co(II), Cr(VI), Hg(II), and Ni(II) are concentrated in a 34-kb region on pMOL28, and genes involved in resistance to Ag(I), Cd(II), Co(II), Cu(II), Hg(II), Pb(II), and Zn(II) occur in a 132-kb region on pMOL30. We identified three putative genomic islands containing metal resistance operons flanked by mobile genetic elements, one on pMOL28 and two on pMOL30. Transcriptomic analysis using quantitative PCR and microarrays revealed metal-mediated up-regulation of 83 genes on pMOL28 and 143 genes on pMOL30 that coded for all known heavy metal resistance proteins, some new heavy metal resistance proteins (czcJ, mmrQ, and pbrU), membrane proteins, truncated transposases, conjugative transfer proteins, and many unknown proteins. Five genes on each plasmid were down-regulated; for one of them, chrI localized on pMOL28, the down-regulation occurred in the presence of five cations. We observed multiple cross-responses (induction of specific metal resistance by other metals), suggesting that the cellular defense of C. metallidurans against heavy metal stress involves various regulons and probably has multiple stages, including a more general response and a more metal-specific response.

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Shamim, Saba, Abdul Rehman, and Mahmood Hussain Qazi. "Swimming, Swarming, Twitching, and Chemotactic Responses of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 in the Presence of Cadmium." Archives of Environmental Contamination and Toxicology 66, no. 3 (2013): 407–14. http://dx.doi.org/10.1007/s00244-013-9966-5.

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Bersch, Beate, Adrien Favier, Paul Schanda, et al. "Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge." Journal of Molecular Biology 380, no. 2 (2008): 386–403. http://dx.doi.org/10.1016/j.jmb.2008.05.017.

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Urbina, Patricia, Beate Bersch, Fabien De Angelis, et al. "Structural and Functional Investigation of the Ag+/Cu+ Binding Domains of the Periplasmic Adaptor Protein SilB from Cupriavidus metallidurans CH34." Biochemistry 55, no. 20 (2016): 2883–97. http://dx.doi.org/10.1021/acs.biochem.6b00022.

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Sarret, Géraldine, Adrien Favier, Jacques Covès, Jean-Louis Hazemann, Max Mergeay, and Beate Bersch. "CopK from Cupriavidus metallidurans CH34 Binds Cu(I) in a Tetrathioether Site: Characterization by X-ray Absorption and NMR Spectroscopy." Journal of the American Chemical Society 132, no. 11 (2010): 3770–77. http://dx.doi.org/10.1021/ja9083896.

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Byloos, Bo, Harsh Maan, Rob Van Houdt, Nico Boon, and Natalie Leys. "The Ability of Basalt to Leach Nutrients and Support Growth of Cupriavidus metallidurans CH34 Depends on Basalt Composition and Element Release." Geomicrobiology Journal 35, no. 5 (2018): 438–46. http://dx.doi.org/10.1080/01490451.2017.1392650.

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Leroy, Baptiste, Caroline Rosier, Vanessa Erculisse, Natalie Leys, Max Mergeay, and Ruddy Wattiez. "Differential proteomic analysis using isotope-coded protein-labeling strategies: Comparison, improvements and application to simulated microgravity effect on Cupriavidus metallidurans CH34." PROTEOMICS 10, no. 12 (2010): 2281–91. http://dx.doi.org/10.1002/pmic.200900286.

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Bersch, Beate, Kheiro-Mouna Derfoufi, Fabien De Angelis, et al. "Structural and Metal-Binding Characterization of the C-terminal Metallochaperone Domain of the Membrane Fusion Protein SilB from Cupriavidus Metallidurans CH34." Biophysical Journal 98, no. 3 (2010): 247a—248a. http://dx.doi.org/10.1016/j.bpj.2009.12.1346.

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Hobman, Jon L., Daniel J. Julian, and Nigel L. Brown. "Cysteine coordination of Pb(II) is involved in the PbrR-dependent activation of the lead-resistance promoter, PpbrA, from Cupriavidus metallidurans CH34." BMC Microbiology 12, no. 1 (2012): 109. http://dx.doi.org/10.1186/1471-2180-12-109.

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