Journal articles on the topic 'Curry, J. L. M'

Murraya koenigii (L.) Spreng., a small, strong-smelling umbrageous tree with subcampanulate white flowers belonging to the family Rutaceae, is native to India and southeastern Asia (2). It is distributed across the Indian subcontinent excluding the higher elevations of the Himalayas. In India, the leaves are mainly used for culinary purposes. The leaves are commonly known as curry leaves or ‘sweet neem’. The whole plant including bark, root, leaves, fruits, and fruit pulp is used medicinally. This plant was reported to be a host of Citrus tristeza virus (1). In a survey of potyvirus incidence in the northwestern Himalaya foothills of the Kangra and Hamirpur districts in the state of Himachal Pradesh in 2004, M. koenigii plants showing mosaic symptoms on leaves, typical of a virus infection, were frequently observed. Symptomatic leaves were tested for the presence of several viruses using enzyme-linked immunosorbent assay with specific antibodies. Positive results were obtained with potyvirus group specific antibodies (Agdia, Elkhardt, IN) in triplicate analyses of 5 of 15 leaf samples tested. To further identify the infecting virus, RNA from plants was tested using universal potyvirus primer pair P9502 and CPUP (3) and reverse transcription-polymerase chain reaction to amplify a genome fragment encoding portions of the coat protein and the 3′UTR (3). An amplification product of the expected size (~800 bp) was obtained. The product was cloned into the pGem-T easy vector (Promega, Madison, WI), and three clones were sequenced. The sequence (GenBank Accession No. AJ852504) had 92% identity to Chili vein banding mottle virus, a potyvirus infecting pepper reported from Thailand (GenBank Accession No. U72193). To our knowledge, this is the first report of a potyvirus naturally occurring on a Murraya sp. References: (1) K. Balaram and K. Ramakrishnan. Curr. Sci. 48:453, 1979. (2) J. D. Hooker. Flora British India 1:502, 1875. (3) R. A. A. van der Vlugt et al. Phytopathology. 89:148, 1999.

Abstract Shin, Y-J., Shannon, L. J., Bundy, A., Coll, M., Aydin, K., Bez, N., Blanchard, J. L., Borges, M. F., Diallo, I., Diaz, E., Heymans, J. J., Hill, L., Johannesen, E., Jouffre, D., Kifani, S., Labrosse, P., Link, J. S., Mackinson, S., Masski, H., Möllmann, C., Neira, S., Ojaveer, H., ould Mohammed Abdallahi, K., Perry, I., Thiao, D., Yemane, D., and Cury, P. M. 2010. Using indicators for evaluating, comparing, and communicating the ecological status of exploited marine ecosystems. 2. Setting the scene. – ICES Journal of Marine Science, 67: 692–716. Background is provided to the selection of ecological indicators by the IndiSeas Working Group, and the methodology adopted for analysis and comparison of indicators across exploited marine ecosystems is documented. The selected indicators are presented, how they are calculated is explained, and the philosophy behind the comparative approach is given. The combination of selected indicators is intended to reflect different dynamics, tracking processes that display differential responses to fishing, and is meant to provide a complementary means of assessing marine ecosystem trends and states. IndiSeas relied on inputs and insights provided by the local experts from participating ecosystems, helping to understand state and trend indicators and to disentangle the effect of other potential ecosystem drivers, such as climate variability. This project showed that the use of simple and available indicators under an ecosystem approach can achieve a real, wide-reaching evaluation of marine ecosystem status caused by fishing. This is important because the socio-economics of areas where fishing activities develop differs significantly around the globe, and in many countries, insufficient data are available for complex and exhaustive analyses.

The paper focused on the description of the reduced graphene oxide (rGO) structure. This material is obtained from a multistage production process. Each of these stages has a large impact on its structure (the number and type of functional groups, number of defect or the size of the flakes), and this in turn affects its properties. We would like to visualize the reduced graphene oxide, both using a diagram showing the atomic structure, as well as by imaging using scanning electron microscopy (SEM) and atomic force microscopy (AFM). In the paper, the elementary composition of selected elements and data obtained from X-ray photoelectron spectroscopy technique (XPS) will be also presented. Full Text: PDF ReferencesX. Peng, Y. Wu, N. Chen, Z. Zhu, J. Liu, and H. Wang, "Facile and highly efficient preparation of semi-transparent, patterned and large-sized reduced graphene oxide films by electrochemical reduction on indium tin oxide glass surface", Thin Solid Films 692, 137626 (2019). CrossRef L. Guo, Y.-W. Hao, P.-L. Li, J.-F. Song, R.-Z. Yang, X.-Y. Fu, S.-Y. Xie, J. Zhao and Y.-L. Zhang, "Improved NO2 Gas Sensing Properties of Graphene Oxide Reduced by Two-beam-laser Interference", Sci. Rep. 8, 1 (2018). CrossRef Y. S. Milovanov, V.A. Skryshevsky, , O.M. Slobodian, , D.O. Pustovyi, X.Tang, J.-P. Raskin, and A.N. Nazarov, "Influence of Gas Adsorption on the Impedance of Graphene Oxide", 2019 IEEE 39th Int. Conf. Electron. Nanotechnology, ELNANO 2019 - Proc. 8783946, CrossRef M. Reddeppa, B.-G. Park, , M.-D. Kim, K.R. Peta, N.D. Chinh, D. Kim, S.-G. Kim, and G. Murali, "H2, H2S gas sensing properties of rGO/GaN nanorods at room temperature: Effect of UV illumination", Sensors Actuators B. Chem. 264, (2018). CrossRef W. L. Xu, C. Ding, , M.-S. Niu, X.-Y. Yang, F. Zheng, J. Xiao, M. Zheng and X.-T. Hao, "Reduced graphene oxide assisted charge separation and serving as transport pathways in planar perovskite photodetector", Org. Electron. 81, 105663 (2020). CrossRef K. Sarkar, M. Hossain, P. Devi, K. D. M. Rao, and P. Kumar, "Self‐Powered and Broadband Photodetectors with GaN: Layered rGO Hybrid Heterojunction", Adv. Mater. Interfaces, 6, 20 (2019). CrossRef S. Pei and H. M. Cheng, "The reduction of graphene oxide", Carbon, 50, 9 (2012). CrossRef R. Muzyka, S. Drewniak, T. Pustelny, M. Chrubasik, and G. Gryglewicz, "Characterization of Graphite Oxide and Reduced Graphene Oxide Obtained from Different Graphite Precursors and Oxidized by Different Methods Using Raman Spectroscopy", Materials 11, 7 (2018). CrossRef M.-H. Tran and H. K. Jeong, "Influence of the Grain Size of Precursor Graphite on the Synthesis of Graphite Oxide", New Phys. Sae Mulli, 63, 2 (2013). CrossRef M.-H. Tran, C.-S. Yang, S. Yang, I.-J. Kim, and H. K. Jeong, "Influence of graphite size on the synthesis and reduction of graphite oxides", Curr. Appl. Phys., 14, SUPPL. 1 (2014). CrossRef N. Sharma, Y. Jain, , M. Kumari, R. Gupta, S.K. Sharma, K. Sachdev, "Synthesis and Characterization of Graphene Oxide (GO) and Reduced Graphene Oxide (rGO) for Gas Sensing Application", Macromol. Symp. 376, 1 (2017). CrossRef M. Wei, L. Qiao, , H. Zhang, S. Karakalos, K. Ma, Z. Fu, M.T. Swihart, G. Wu, "Engineering reduced graphene oxides with enhanced electrochemical properties through multiple-step reductions", Electrochim. Acta, 258 (2017). CrossRef S. Drewniak, M. Procek, R. Muzyka, T. Pustelny, "Comparison of Gas Sensing Properties of Reduced Graphene Oxide Obtained by Two Different Methods", Sensors, 20, 11 (2020). CrossRef L. Li, R.-D. Lv, S. -C. Liu, Z. D. Chen, J. Wang, Y.-G. Wang, W. Ren, "Using Reduced Graphene Oxide to Generate Q-Switched Pulses in Er-Doped Fiber Laser", Chinese Physics Letters, 35, 11 (2018) CrossRef

On 16 December 2015 a severe thunderstorm and associated tornado affected Sydney causing widespread damage and insured losses of $206 million. Severe impacts occurred in Kurnell, requiring repairs to Sydney's desalination plant which supplies up to 15% of Sydney water during drought, with repairs only completed at the end of 2018. Climatologically, this storm was unusual as it occurred during the morning and had developed over the ocean, rather than developing inland during the afternoon as is the case for many severe storms impacting the Sydney region. Simulations of the Kurnell storm were conducted using the Weather Research and Forecasting (WRF) model on a double nested domain using the Morrison microphysics scheme and the NSSL 2-moment 4-ice microphysics scheme. Both simulations produced severe storms that followed paths similar to the observed storm. However, the storm produced under the Morrison scheme did not have the same morphology as the observed storm. Meanwhile, the storm simulated with the NSSL scheme displayed cyclical low- and mid-level mesocyclone development, which was observed in the Kurnell storm, highlighting that the atmosphere supported the development of severe rotating thunderstorms with the potential for tornadogenesis. The NSSL storm also produced severe hail and surface winds, similar to observations. The ability of WRF to simulate general convective characteristics and a storm similar to that observed displays the applicability of this model to study the causes of severe high-impact Australian thunderstorms. References J. T. Allen and E. R. Allen. A review of severe thunderstorms in Australia. Atmos. Res., 178:347–366, 2016. doi:10.1016/j.atmosres.2016.03.011. Bureau of Meteorology. Severe Storms Archive, 2020. URL http://www.bom.gov.au/australia/stormarchive/. D. T. Dawson II, M. Xue, J. A. Milbrandt, and M. K. Yau. Comparison of evaporation and cold pool development between single-moment and multimoment bulk microphysics schemes in idealized simulations of tornadic thunderstorms. Month. Wea. Rev., 138:1152–1171, 2010. doi:10.1175/2009MWR2956.1. H. Hersbach, B. Bell, P. Berrisford, S. Hirahara, A. Horanyi, J. Munoz-Sabater, J. Nicolas, C. Peubey, R. Radu, D. Schepers, et al. The ERA5 global reanalysis. Quart. J. Roy. Meteor. Soc., 146:1999–2049, 2020. doi:10.1002/qj.3803. Insurance Council of Australia. Victorian bushfire losses push summer catastrophe bill past $550m, 2016. E. R. Mansell, C. L. Ziegler, and E. C. Bruning. Simulated electrification of a small thunderstorm with two-moment bulk microphysics. J. Atmos. Sci., 67:171–194, 2010. doi:10.1175/2009JAS2965.1. R. C. Miller. Notes on analysis and severe-storm forecasting procedures of the Air Force Global Weather Central, volume 200. Air Weather Service, 1972. URL https://apps.dtic.mil/sti/citations/AD0744042. H. Morrison, J. A. Curry, and V. I. Khvorostyanov. A new double-moment microphysics parameterization for application in cloud and climate models. Part I: Description. J. Atmos. Sci., 62:1665–1677, 2005. doi:10.1175/JAS3446.1. H. Morrison, G. Thompson, and V. Tatarskii. Impact of cloud microphysics on the development of trailing stratiform precipitation in a simulated squall line: Comparison of one- and two-moment schemes. Month. Wea. Rev., 137:991–1007, 2009. doi:10.1175/2008MWR2556.1. J. G. Powers, J. B. Klemp, W. C. Skamarock, C. A. Davis, J. Dudhia, D. O. Gill, J. L. Coen, D. J. Gochis, R. Ahmadov, S. E. Peckham, et al. The Weather Research and Forecasting Model: Overview, system efforts, and future directions. Bull. Am. Meteor. Soc., 98:1717–1737, 2017. doi:10.1175/BAMS-D-15-00308.1. H. Richter, A. Protat, J. Taylor, and J. Soderholm. Doppler radar and storm environment observations of a maritime tornadic supercell in Sydney, Australia. In Preprints, 28th Conf. on Severe Local Storms, Portland OR, Amer. Meteor. Soc. P, 2016. W. C. Skamarock, J. B. Klemp, J. Dudhia, D. O. Gill, Z. Liu, J. Berner, W. Wang, J. G. Powers, M. G. Duda, D. Barker, and X.-Y. Huang. A description of the advanced research WRF Model version 4. Technical report, 2019. Storm Prediction Center. The Enhanced Fujita Scale (EF Scale), 2014. URL https://www.spc.noaa.gov/efscale/. R. A. Warren, H. A. Ramsay, S. T. Siems, M. J. Manton, J. R. Peter, A. Protat, and A. Pillalamarri. Radar-based climatology of damaging hailstorms in Brisbane and Sydney, Australia. Quart. J. Roy. Meteor. Soc., 146:505–530, 2020. doi:10.1002/qj.3693.

Bacterial leaf scorch caused by Xylella fastidiosa has been reported in 17 species of oak including bur, pin, red, scarlet, shingle, and white oaks (3). In September 2002, a leaf scorch symptom characterized by marginal necrosis of leaves bordered by a darker brown band was observed in a mature black oak (Quercus velutina Lam.) at the U.S. National Arboretum in Washington, D.C. The leaf petiole of the black oak was processed in general extraction buffer (Agdia, Inc., Elkhart, IN) contained in a FastDNA lysing matrix tube using the FastPrep FP120 instrument (Qbiogene, Inc., Carlsbad, CA) (1). The leaf petiole extract reacted with an antiserum specific for X. fastidiosa (Agadia, Inc.) in an enzyme-linked immunosorbent assay (ELISA). A slow-growing bacterium was cultured from leaf petioles of the affected black oak tree by soaking the surface-sterilized, finely cut leaf petioles in sterile water for 30 min, followed by spreading the bacterial suspension on periwinkle wilt plates (1). When the cultured bacterium was subjected to polymerase chain reaction (PCR) with primers specific for X. fastidiosa (2), a 472-bp PCR product was detected. The PCR product was confirmed to be the predicted X. fastidiosa product by sequencing and sequence comparison with the reported genomic sequence of X. fastidiosa. ELISA and bacterial isolation from leaf petioles of a nearby symptomless white oak (Q. alba L.) tree were negative. To our knowledge, this is the first report of X. fastidiosa associated with leaf scorch in black oak in the United States, expanding the host range of the bacterium in economically important landscape tree species. References: (1) Q. Huang and J. L. Sherald. Curr. Microbiol. 48:73, 2004. (2) M. R. Pooler and J. S. Hartung. Curr. Microbiol. 31:377, 1995. (3) J. L. Sherald. Xylella fastidiosa, A bacterial pathogen of landscape trees. Page 191 in: Shade Tree Wilt Diseases, C. L. Ash, ed. The American Phytopathological Society, 2001.

Analysis of optical structures that can work for broadband range of THz radiation is the aim of this article. Such structures can be designed as kinoforms of higher order or elements with extended depth of focus, like axicons or light sword elements. The theoretical and experimental comparison of different optical elements for three significantly different frequencies is performed. Full Text: PDF ReferencesM. C. Kemp, P.F. Taday, B.E. Cole, J.A. Cluff, A.J. Fitzgerald, W.R. Tribe, "Security applications of terahertz technology", International Society for Optics and Photonics, 5070, pp. 44-53 (2003). CrossRef D. Yavorskiy, J. Marczewski, K. Kucharski, et al., "THz Scanner Based on Planar Antenna-Supplied Silicon Field-Effect Transistors", Photonics Letters of Poland, 4(3), 100-102 (2012). CrossRef A.A. Angeluts, A.B. Gapeyev, M.N. Esaulkov, et al., "Study of terahertz-radiation-induced DNA damage in human blood leukocytes", Quantum Electronics, 44(3), 247 (2014). CrossRef Q. Sun, Y. He, K. Liu, S. Fan, E.P. Parrott, E. Pickwell-MacPherson, "Recent advances in terahertz technology for biomedical applications", Quantitative imaging in medicine and surgery, 7(3), 345 (2017). CrossRef J. Suszek, A. Siemion, M. Bieda, et al., "3-D-Printed Flat Optics for THz Linear Scanners", IEEE Thz Sci. T. 5, (2015). CrossRef M. Naftaly, R. Dudley, "Methodologies for determining the dynamic ranges and signal-to-noise ratios of terahertz time-domain spectrometers", Optics Letters, 34(8), 1213-1215 (2009). CrossRef C. Bruckner, G. Notnia, and A. Tünnermann, "Optimal arrangement of 90° off-axis parabolic mirrors in THz setups", Optik, 121, 1 (2010). CrossRef J. Richter, A. Hofmann, L.-P. Schmidt, "Dielectric Wide Angle Lenses for Millimeter-Wave Focal Plane Imaging", Proc. of the 31st European Microwave Conference, London, UK (2001). CrossRef E.D. Walsby, S. M. Durbin, D.R S. Cumming, R.J. Blaikie, "Analysis of silicon terahertz diffractive optics", Curr. App. Phys., 4, (2004). CrossRef J.A. Jordan Jr et al, Appl Opt., 9(8), 1883-1887 (1970) CrossRef J.C. Marron, D.K. Angell, A.M. Tai, "Higher-order kinoforms", International Society for Optics and Photonics, 1211, 62-67 (1990). CrossRef J. Suszek, A.M. Siemion, N. Błocki, M. Makowski, A. Czerwiński, J. Bomba, P. Zagrajek et al., "High order kinoforms as a broadband achromatic diffractive optics for terahertz beams", Optics Express, 22(3), 3137-3144 CrossRef J. Sochacki, A. Kołodziejczyk, Z. Jaroszewicz, S. Bara, "Nonparaxial design of generalized axicons", Applied Optics, 31(25), 5326-5330 (1992). CrossRef A. Kołodziejczyk, S. Bará, Z. Jaroszewicz, M. Sypek, "The Light Sword Optical Element—a New Diffraction Structure with Extended Depth of Focus", Journal of Modern Optics, 37(8), 1283-1286 (1990). CrossRef M. Sypek, "Light propagation in the Fresnel region. New numerical approach", Opt. Commun., 116, 43–48 (1995). CrossRef J.P. Kruth, X. Wang, T. Laoui, L. Froyen, "Lasers and materials in selective laser sintering", Assembly Automation, 23(4), 357-371 (2003). CrossRef

Self-regulation for pre-school children is very important to support children’s adjustments in all situations and conditions. The current problem is the instrument of self-regulation is more focused on regulation in learning which is not suitable for young children. This study aims to examine the validity and reliability of Preschool Self-Regulation Assessment (PSRA) in Indonesia by modifying several children self-regulation theories. The instrument was translated from English into Indonesian and it retranslated into the native language by linguists. The questions, then, were validated through a process of professional judgment and cognitive de-briefing. The study was carried out to 179 children aged 6 to 7 years old. Data were analyzed by using confirmatory factor analysis (CFA). It showed that there are 5 dimensions of children's self-regulation, namely: attentional focus, behavioral control, self-motivated, self-autonomy and emotional control. The result showed that the five-dimensional model is agreed with the data and prove to measure children’s self-regulation. Cronbach’s alpha coefficient value was 0.899, indicating high scale reliability. Thus, the pre-school children’s self-regulation assessment has well psychometric for further use. Keywords: Children’s self-regulation, Confirmatory Factor Analysis, Construct validation, Pre-school self-regulation assessment, Reliability References Amanda, N. ., Antara, P. ., & Magta, M. (2016). Hubungan Pola Asuh Orangtua dengan Regulasi Diri Anak Usia 5-6 Tahun. Journal Pendidikan Anak Usia Dini Universitas Pendidikan Ganesha, 4(2), 1–11. Bentley, J. J. (2013). Parental Involvement, Parent-Child Warmth and School Engagement as Mediated by Self-Regulation. Brigham Young University. Bierman, K. L., Domitrovich, C. E., Nix, R. L., Welsh, J. A., Greenberg, M. T., Blair, C., … Gill, S. (2008). Promoting academic and social-emotional school readiness: The Head Start REDI program. Child Development, 79(6). Blair, C., & Diamond. (2008). Developing your Children Self-Regulation through Everyday Experiences. Blair, C., & Raver, C. C. (2015). School readiness and self-regulation: A developmental psychobiological approach. Annual Review of Psychology, 66, 711–731. Eisenberg, N., Hofer, C., & Vaughan, J. (2007). Effortful Control and Its Socioemotional Consequences. In J. J. Gross (Ed.), Handbook of emotion regulation (hal. 287–306). New York: Guilford Press. Eisenberg, N., Spinrad, T. L., & Eggum, N. D. (2010). Emotion-Related Self-Regulation and Its Relation to Children’s Maladjustment. Annual Reviews Clinical Psychology, 27(6), 495–525. Eisenberg, N., Valiente, C., & D.Eggum, N. (2010). Self-Regulation and School Readiness. Early Education Development., 21(5), 681–698. Goyette, P., Carrol, K., Smith-Donald, R., Metzger, M., Young, T., & Raver, C. C. (2006). Field Administration of an Emotional and Behavioral Assessment of Head Start Children:Preliminary Findings from the Preschool Self-Regulation Assessment. Grolnick, W. S., & Farkas, M. (2002). Parenting and the development of children’s self-regulation. In M. H. Bornstein (Ed.), Handbook of parenting (Vol. 5, hal. 89–110). Practical issues in parenting. Pino, D., & Whitebread, D. (2010). The Role of Parenting in Children’s Self-Regulated Learning. Educational Research Review, 5(3), 220–242. Raver, C. C., Jones, S. M., Li-Grining, C., Zhai, F., Bub, K., & Pressler, E. (2011). CSRP’s impact on low-income preschoolers’ pre-academic skills: Self-regulation and teacher-student relationships as two mediating mechanisms. Child Development, 82(1), 362–378. Rimm-Kaufman, S. E., Curby, T. W., Grimm, K. J., Nathanson, L., & Brock, L. L. (2009). The contribution of children’s self-regulation and classroom quality to children’s adaptive behaviors in the kindergarten classroom. Developmental Psychology, 45(4). Rochmah, S. N. (2017). Hubungan Konsep Diri Guru Terhadap Regulasi Diri Anak Usia Dini. Jurnal Tunas Siliwangi SPS UPI, 3(2), 160–174. Smith-Donald, R., Carroll, K., Goyette, P., Metzger, M., Young, T., & Raver, C. C. (2006). Preliminary Validity of the Preschool Self-Regulation Assessment (PSRA). Smith-Donald, R., Raver, C. C., Hayes, T., & Richardson, B. (2007). Preliminary construct and concurrent validity of the Preschool Self-regulation Assessment (PSRA) for field-based research. Early Childhood Research Quarterly, 22(2), 173–187. Tanribuyurdu, Findik, E., Yildiz, & Guler, T. (2014). Preschool Self-Regulation Assessment (PSRA): Adaptation Study for Turkey. Education and Science, 39(176), 317–328. Wang, L., Hamaker, E., & Bergeman, C. (2014). Investigating inter-individual differences in short-term intra-individual variability. Psychological Methods, 17(4), 2012. Zimmerman, B. (2002). Becoming a Self-Regulated Learner: An Overview. Theory Into Practice, 41(2), 64–70

ABSTRACT The diketo acid L-708,906 has been reported to be a selective inhibitor of the strand transfer step of the human immunodeficiency virus type 1 (HIV-1) integration process (D. Hazuda, P. Felock, M. Witmer, A. Wolfe, K. Stillmock, J. A. Grobler, A. Espeseth, L. Gabryelski, W. Schleif, C. Blau, and M. D. Miller, Science 287:646-650, 2000). We have now studied the development of antiviral resistance to L-708,906 by growing HIV-1 strains in the presence of increasing concentrations of the compound. The mutations T66I, L74M, and S230R emerged successively in the integrase gene. The virus with three mutations (T66I L74M S230R) was 10-fold less susceptible to L-708,906, while displaying the sensitivity of the wild-type virus to inhibitors of the RT or PRO or viral entry process. Chimeric HIV-1 strains containing the mutant integrase genes displayed the same resistance profile as the in vitro-selected strains, corroborating the impact of the reported mutations on the resistance phenotype. Phenotypic cross-resistance to S-1360, a diketo analogue in clinical trials, was observed for all strains. Interestingly, the diketo acid-resistant strain remained fully sensitive to V-165, a novel integrase inhibitor (C. Pannecouque, W. Pluymers, B. Van Maele, V. Tetz, P. Cherepanov, E. De Clercq, M. Witvrouw, and Z. Debyser, Curr. Biol. 12:1169-1177, 2002). Antiviral resistance was also studied at the level of recombinant integrase. Single mutations did not appear to impair specific enzymatic activity. However, 3′ processing and strand transfer activities of the recombinant integrases with two (T66I L74M) and three (T66I L74M S230R) mutations were notably lower than those of the wild-type integrase. Although the virus with three mutations was resistant to inhibition by diketo acids, the sensitivity of the corresponding enzyme to L-708,906 or S-1360 was reduced only two- to threefold. As to the replication kinetics of the selected strains, the replication fitness for all strains was lower than that of the wild-type HIV-1 strain.

Two Permian-Triassic boundary (PTB) successions, Lung Cam in Vietnam, and Lukač in Slovenia, have been sampled for high-resolution magnetic susceptibility, stable isotope and elemental chemistry, and biostratigraphic analyses. These successions are located on the eastern (Lung Cam section) and western margins (Lukač section) of the Paleo-Tethys Ocean during PTB time. Lung Cam, lying along the eastern margin of the Paleo-Tethys Ocean provides an excellent proxy for correlation back to the GSSP and out to other Paleo-Tethyan successions. This proxy is tested herein by correlating the Lung Cam section in Vietnam to the Lukač section in Slovenia, which was deposited along the western margin of the Paleo-Tethys Ocean during the PTB interval. It is shown herein that both the Lung Cam and Lukač sections can be correlated and exhibit similar characteristics through the PTB interval. Using time-series analysis of magnetic susceptibility data, high-resolution ages are obtained for both successions, thus allowing relative ages, relative to the PTB age at ~252 Ma, to be assigned. Evaluation of climate variability along the western and eastern margins of the Paleo-Tethys Ocean through the PTB interval, using d18O values indicates generally cooler climate in the west, below the PTB, changing to generally warmer climates above the boundary. A unique Black Carbon layer (elemental carbon present by agglutinated foraminifers in their test) below the boundary exhibits colder temperatures in the eastern and warmer temperatures in the western Paleo-Tethys Ocean.ReferencesBalsam W., Arimoto R., Ji J., Shen Z, 2007. Aeolian dust in sediment: a re-examination of methods for identification and dispersal assessed by diffuse reflectance spectrophotometry. International Journal of Environment and Health, 1, 374-402.Balsam W.L., Otto-Bliesner B.L., Deaton B.C., 1995. 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During April 2009, a commercial spinach field (1 km2 [250 acres]) in south-central Arizona developed geminivirus-like disease symptoms (4). Approximately 40 to 50% of the spinach plants exhibited extreme leaf distortion, foliar interveinal chlorosis, shortened internodes, and ~80% yield reduction. The beet leafhopper, Circulifer tenellus, the only known insect vector of curtoviruses in the United States, was observed on spinach plants. Total DNA was isolated (1) from three plant samples exhibiting the same symptom phenotype and used to PCR-amplify a 446-bp fragment of a suspected curtovirus, using primers F 5′-CTACCATCAGTAATGATGGG-3′and R 5′ CATATTTGCCACCTCCAGTGTC-3′ designed around the coat protein gene (Cp) for several known curtoviruses. DNA sequencing and BLAST analysis of the cloned fragments (n = 3 with 100% identity) revealed BLAST matches at 81 to 83% with the Cp for three isolates of Beet curly top Iran virus (BCTIV) (EU273816–18). To amplify the full-length curtovirus genome, total DNA from one of the three positive samples was used as the template in rolling circle amplification (RCA) employing the non-sequence specific TempliPhi 100 Amplification System (GE Healthcare) that amplifies circular DNA templates. The RCA products were linearized with PstI, yielding a ~3-Kbp fragment that was cloned into pGEM3zf+ (Promega, Madison, WI). To obtain the complete sequence, one plasmid (09-10-8) containing a full-length insert was selected and prepared for sequencing with the Template Generation System II Kit (Finnzymes, Espoo, Finland). The resultant 28 sequences were assembled into a contig using SeqMan software (DNASTAR, Madison, WI). Also, RCA clones (09.10-2, -3, and -4) from the same sample were subjected to DNA sequencing with universal M13F and M13R primers followed by primer walking (>300 bp overlap). The four 3,066-bp genomes shared 99 to 100% nt identity. An alignment (ClustalV; MegAlign, DNASTAR) with sequences of all curtovirus species available in GenBank indicated that the Arizona spinach isolates shared the highest nt sequence identity (59%) with Horseradish curly top virus (HrCTV). The next closest relatives were Beet mild curly top virus, Beet severe curly top virus, and Spinach curly top virus, at 50%. The genome consists of six open reading frames and lacks the AC3 gene, an arrangement most similar to HrCTV (3). The ICTV approved working cut-off for Curtovirus species demarcation at <89% nt identity (2) supports recognition of this isolate from spinach (GU734126) as a new, previously undescribed curtovirus species, for which we propose the name Spinach severe curly top virus (SSCTV-[Arizona:2009]). The curtovirus-like symptoms, presence of the curtovirus leafhopper vector, and isolation of a curtovirus-like genome from symptomatic spinach plants are highly suggestive of curtovirus etiology. To our knowledge, this is the first report of SSCTV worldwide and its association with diseased spinach in Arizona. Although a different curtovirus species was reported from the same infected spinach field (4), this study provides evidence that at least two curtoviruses were present in this spinach field in Arizona. References: (1) J. J. Doyle and J. L. Doyle. Focus 12:13, 1990. (2) C. M. Fauquet et al. Arch. Virol. 153:783, 2008. (3) K. A. Klute et al. J. Gen. Virol. 77:1369, 1996. (4) C. Nischwitz and M. W. Olsen. Online publication. doi:10.1094/PHP-2010-0216-01-BR. Plant Health Progress. 2010.

The diversity of forms in multicellular organisms originates largely from the spatial redeployment of developmental genes [S. B. Carroll,Cell134, 25–36 (2008)]. Several scenarios can explain the emergence ofcis-regulatory elements that govern novel aspects of a gene expression pattern [M. Rebeiz, M. Tsiantis,Curr. Opin. Genet. Dev.45, 115–123 (2017)]. One scenario, enhancer co-option, holds that a DNA sequence producing an ancestral regulatory activity also becomes the template for a new regulatory activity, sharing regulatory information. While enhancer co-option might fuel morphological diversification, it has rarely been documented [W. J. Glassford et al.,Dev. Cell34, 520–531 (2015)]. Moreover, if two regulatory activities are borne from the same sequence, their modularity, considered a defining feature of enhancers [J. Banerji, L. Olson, W. Schaffner,Cell33, 729–740 (1983)], might be affected by pleiotropy. Sequence overlap may thereby play a determinant role in enhancer function and evolution. Here, we investigated this problem with two regulatory activities of theDrosophilageneyellow, the novelspotenhancer and the ancestralwing bladeenhancer. We used precise and comprehensive quantification of each activity inDrosophilawings to systematically map their sequences along the locus. We show that thespotenhancer has co-opted the sequences of thewing bladeenhancer. We also identified a pleiotropic site necessary for DNA accessibility of a shared regulatory region. While the evolutionary steps leading to the derived activity are still unknown, such pleiotropy suggests that enhancer accessibility could be one of the molecular mechanisms seeding evolutionary co-option.

Integrins are heterodimeric cell surface receptors that mediate heterophilic cell-cell interactions and interactions between cells and the extracellular matrix (Hynes RO. Cell 69: 11–25, 1991). As such, they are involved in morphogenetic processes during development, as well as in the maintenance of normal tissue architecture in fully developed organs. Integrins are now recognized to be a large family of receptors, and several different integrins have been demonstrated as being expressed in the developing and adult kidney (Korhonen M, Ylkanne J, Laitinen L, and Virtanen I. Development 122: 3537–3547, 1996; Rahilly MA and Fleming S. J Pathol 167: 327–334, 1992). This review will summarize present knowledge about integrin expression in the developing, normal, and diseased kidney and attempt to provide a hypothetical framework for understanding integrin function in the urogenital system. Since the last time this area was reviewed (Hamerski DA and Santoro S. Curr Opin Nephrol Hypertens 8: 9–14, 1999), there have been significant publications on the roles of integrins in kidney development and disease. At present, there are many more questions than answers, and integrins present an area where many novel and exciting findings will emerge in the coming years.

Background:Clinical improvement after B-cell depletion with rituxmab suggests a prominent pathogenic role of B-lymphcytes in IgG4-related disease (IgG4-RD). IgG4-RD, however, relapses in most cases together with re-expansion of clonally divergent plasmablasts indicating that treatment with rituximab does not completely abrogates T follicular helper (Tfh)-cells dependent germinal center reactions leading to de-novo plasmablast differentiation.Objectives:In the present work we aim to study the effects of B-cell depletion therapy with rituximab on circulating Tfh cells and on the levels of CXCL13 - a chemotactic factor for B-lymphocytes produced by Tfh cells - in patients with IgG4-RD.Methods:Thirty patients with IgG4-RD, diagnosed according to the “Consensus Statement on the Pathology of IgG4-RD” and fulfilling the “2019 ACR/EULAR Classification Criteria” were included in the present study. Ten patients with relapsing disease were treated with the anti-CD20 monoclonal antibody rituximab (two 1g infuxions 15 days apart). Peripheral blood mononuclear cells and serum were collected before rituximab and three months after infusion. Tfh cells subsets in the peripheral blood were measured by flow cytometry and CXCL13 plasma levels were measured by ELISA assay.Results:No changes in total Tfh cells and Tfh cells subsets were observed three months after rituximab neither in absolute counts nor in percentage of CD4+ T cells. In particular, no difference in Tfh1, Tfh2, Tfh17, T follicular regulatory and highly functional Tfh cells counts was observed before and after treatment. The serum level of CXCL13 was significantly higher in active untreated IgG4-RD patients compared to healthy controls (151.94 pg/ml vs 66.98 pg/ml, p value = 0.0026), but was not affected by rituximab treatment (p value = 0.41).Conclusion:In relapsing patients with IgG4-RD rituximab does not affect circulating Tfh cells numbers and serum levels of CXCL13. Persistence of Tfh cells after rituximab and reconstitution of germinal center reactions likely drives IgG4-RD flare.References:[1]Lanzillotta M, Mancuso G, Della-Torre E. Advances in the diagnosis and management of IgG4 related disease. BMJ. 2020 Jun 16;369:m1067. doi: 10.1136/bmj.m1067. PMID: 32546500.[2]Lanzillotta M, Della-Torre E, Stone JH. Roles of Plasmablasts and B Cells in IgG4-Related Disease: Implications for Therapy and Early Treatment Outcomes. Curr Top Microbiol Immunol. 2017;401:85-92. doi: 10.1007/82_2016_58. PMID: 28091934.[3]Campochiaro C, Ramirez GA, Bozzolo EP, Lanzillotta M, Berti A, Baldissera E, Dagna L, Praderio L, Scotti R, Tresoldi M, Roveri L, Mariani A, Balzano G, Castoldi R, Doglioni C, Sabbadini MG, Della-Torre E. IgG4-related disease in Italy: clinical features and outcomes of a large cohort of patients. Scand J Rheumatol. 2016;45(2):135-45. doi: 10.3109/03009742.2015.1055796. Epub 2015 Sep 23. PMID: 26398142.[4]Mattoo H, Mahajan VS, Della-Torre E, Sekigami Y, Carruthers M, Wallace ZS, Deshpande V, Stone JH, Pillai S. De novo oligoclonal expansions of circulating plasmablasts in active and relapsing IgG4-related disease. J Allergy Clin Immunol. 2014 Sep;134(3):679-87. doi: 10.1016/j.jaci.2014.03.034. Epub 2014 May 6. PMID: 24815737; PMCID: PMC4149918.Disclosure of Interests:None declared

ABSTRACTEpistatic interactions in which the phenotypic effect of an allele is conditional on its genetic background have been shown to play a central part in various evolutionary processes. In a previous study (J. B. Anderson et al., Curr. Biol. 20:1383-1388, 2010; J. R. Dettman, C. Sirjusingh, L. M. Kohn, and J. B. Anderson, Nature 447:585-588, 2007), beginning with a common ancestor, we identified three determinants of fitness as mutant alleles (each designated with the letter “e”) that arose in replicateSaccharomyces cerevisiaepopulations propagated in two different environments, a low-glucose and a high-salt environment. In a low-glucose environment,MDS3eandMKT1einteracted positively to confer a fitness advantage. Also,PMA1efrom a high-salt environment interacted negatively withMKT1ein a low-glucose environment, an example of a Dobzhansky-Muller incompatibility that confers reproductive isolation. Here we showed that the negative interaction betweenPMA1eandMKT1eis mediated by alterations in intracellular pH, while the positive interaction betweenMDS3eandMKT1eis mediated by changes in gene expression affecting glucose transporter genes. We specifically addressed the evolutionary significance of the positive interaction by showing that the presence of theMDS3mutation is a necessary condition for the spread and fixation of the new mutations at the identical site inMKT1. The expected mutations inMKT1rose to high frequencies in two of three experimental populations carryingMDS3ebut not in any of three populations carrying the ancestral allele. These data show how positive and negative epistasis can contribute to adaptation and reproductive isolation.

USO RACIONAL DA ÁGUA E DA ADUBAÇÃO NITROGENADA NO MILHO (Zea Mays L.) IRRIGADO POR GOTEJAMENTO EM CULTIVO DE OUTONO/INVERNO1 PAULO FERREIRA DA SILVA2; MARCOS ANTONIO LIODORO DOS SANTOS3; JOÃO VICTOR RIBEIRO DA SILVA DE SOUZA4; DOUGLAS MARCELO PINHEIRO DA SILVA5; JÉSSICA MAIARA DE SOUZA FERRARI6 E JOÃO CARLOS CURY SAAD7 1 Trabalho retirado da tese intitulada:” Manejo da irrigação e da adubação nitrogenada no milho cultivado no outono/inverno em Botucatu-SP”, do autor Paulo Ferreira da Silva2 2 Doutor em Agronomia Irrigação e Drenagem, Rua Elpídio Coelho, 427, Centro, 64750-000, Paulistana, PI, Brasil, e-mail: pauloagrom@gmail.com 3 Doutor em Agronomia, Departamento de vendas e projetos, Irrimar irrigação e serviços LTDA, Av. Antares 720, Recanto dos Vinhais, 65070-070, São Luís, MA, Brasil, e-mail: marcos.liodorio@irrimar.com.br 4 Doutor em Agronomia Irrigação e Drenagem, Departamento de Engenharia rural, FCA - UNESP, Rua José Barbosa de Barros, 1780, Fazenda Lageado, 18603-970, Botucatu, SP, Brasil, e-mail: joao.vrsp@gmail.com 5 Professor Doutor, Instituto de Educação Agricultura e Ambiente, Universidade Federal do Amazonas, Rua 29 de Agosto, 786, Centro. 69800-000, Humaitá, AM, Brasil, e-mail: douglassilva@ufam.edu.com 6 Mestre em Agronomia Irrigação e Drenagem, Faculdade de Ciências e Engenharia, UNESP, Rua Domingos da Costa Lopes, 780, Jardim Itaipu, 17602-496, Tupã, SP, Brasil, e-mail: jessicamaiaraferrari@gmail.com 7 Professor Doutor, Departamento de Engenharia Rural e Socioeconomia, FCA - UNESP, Av. Universitária, 3780, Altos do Paraíso,18610-034, Botucatu, SP, Brasil, e-mail: joao.saad@unesp.br 1 RESUMO Este estudo foi realizado para investigar o efeito da irrigação e doses de fertilizante nitrogenado sobre a produtividade do milho cultivado entre os meses de abril a outubro de 2016 e 2017. O experimento foi conduzido na Fazenda Lageado na área experimental localizada nas coordenadas geodésicas 22°51'07" Sul, 48°25'45,07" Oeste e altitude de 764 m. A acidez do solo foi corrigida com calcário dolomítico apenas no ano de 2016 e a semeadura realizada no espaçamento de 0,85 x 0,20 m entre as linhas e plantas, respectivamente, com adição de 300 kg de NPK 04-30-10 aplicado no sulco. O delineamento experimental usado foi em blocos casualizados, em esquema fatorial 5 x 5, com parcela subdividida sendo cinco níveis de irrigação correspondendo a 50, 75, 100, 125 e 150% da evapotranspiração diária da cultura (ETc) e cinco doses de adubação nitrogenada nos níveis 0, 22,5, 45, 67,5 e 90 kg ha-1 de N, utilizando a uréia como fonte de N em quatro repetições. A produtividade de grãos de milho foi maior utilizando a lâmina com 100% da ETc nos anos de 2016 e 2017. Para o fator adubação nitrogenada, o maior rendimento foi obtido no nível utilizando 90 kg ha-1 de N. Palavras-chave: economia de água, irrigação suplementar, produtividade de grãos SILVA, P. F; SANTOS, M. A. L; SOUZA, J. V. R. S.; SILVA, D. A. P.; FERRARI, J. M. S.; SAAD, J. C. C. RATIONAL USE OF WATER AND NITROGENATED FERTILIZATION IN CORN (Zea Mays L.) IRRIGATED BY DRIPPING IN AUTUMN / WINTER CULTIVATION 2 ABSTRACT This study was carried out to investigate the effect of irrigation and nitrogen fertilizer doses on the productivity of corn grown between April and October 2016 and 2017. The experiment was conducted at Fazenda Lageado in the experimental area located at geodetic coordinates 22°51'07" South, 48°25'45.07" West and altitude of 764 m. The soil was corrected with dolomitic limestone only in 2016 and the sowing carried out at a spacing of 0.85 x 0.20 m between the lines and plants, respectively, with the addition of 300 kg of NPK 04-30-10 applied in the furrow. The experimental design used was randomized blocks, in a 5 x 5 factorial scheme, with a subdivided plot with five levels of irrigation corresponding to 50, 75, 100, 125 and 150% of the daily culture evapotranspiration (ETc) and five doses of nitrogen fertilization at levels 0; 22.5; 45; 67.5 and 90 kg ha-1 of N, using urea as a source of N in four replications. The productivity of corn grains was higher using depth with 100% ETc in the years 2016 and 2017. For the nitrogen fertilization factor, the highest yield was obtained at the level using 90 kg ha-1 of N. Keywords: water saving, supplementary irrigation, grain yield

This research is aimed to discover the interrelation between marital satisfaction, religiouscommitment and marital commitment globally and based on its types, personal, moral, andstructural. The sampling of the research is 315 persons, with age 20 until 58 years old. The resultof the research shows there is a significant correlation between marital satisfaction and maritalcommitment, religious commitment and marital commitment, marital satisfaction together withreligious commitment and marital commitment. It is also discovers that marital commitmentinfluences personal commitment and moral commitment, while religious commitment influencespersonal commitment, moral commitment, and structural commitment.Keywords: marital satisfaction, religious commitment, marital commitment Abstrak Penelitian ini bertujuan untuk mengetahui hubungan antara kepuasan pernikahan, komitmen beragama, dan komitmen pernikahan secara global dan menurut tipenya, komitmen personal, moral, dan struktural. Partisipan penelitian ini adalah berjumlah 315 orang, berusia 20 hingga 58 tahun. Hasil penelitian ini menunjukan bahwa terdapat hubungan signifikan antara kepuasan pernikahan dan komitmen pernikahan, komitmen beragama dan komitmen pernikahan, kepuasan pernikahan bersama dengan komitmen beragama dan komitmen pernikahan. Juga diketahui bahwa kepuasan pernikahan memiliki pengaruh terhadap komitmen personal dan komitmen moral. Sementara komitmen beragama memiliki pengaruh terhadap komitmen personal, komitmen moral, dan komitmen struktural. Kata kunci: kepuasan pernikahan, komitmen beragama, komitmen pernikahan References Abbott, D., Berry, M. and Meredith, W. (1990). Religious Belief and Practice: A Potential Assetin Helping Families. Family Relations, 39(4), p.443.Adams, J. M. & Jones, W. H. (1999). Interpersonal commitment in historichal perspectives. InHandbook of Interpersonal Commitment and Relationship Stability. New York: SpringerScience+Business Media.Agnew, H. (2009). Commitment, Theories and Typologies. Department of PsychologicalSciences Faculty Publications. Diunduh dari: http://docs.lib.purdue.edu/psychpubs/28Allgood, S. M., Harris, S.,Skogrand, L., & Lee, T.R. (2008). Marital commitment andreligiosity in a religiously homogenous population. Marriage & Family Review, 45(1),52-67. doi: 10.1080/01494920802537472.Amato, P. R. 2010. Research on divorce: continuing developments and newtrends. Journal ofMarriage and Family 72(3): 650-666. doi:10.1111/j.1741-3737.2010.00723.xAmato, P. and Sobolewski, J. (2001). The Effects of Divorce and Marital Discordon AdultChildren's Psychological Well-Being. American SociologicalReview, 66(6), p.900.Andrea, S.G. (2014). Hubungan antara religiositas dan komitmen pernikahan pada individuyang menikah melalui ta’aruf. Skripsi. Depok: Fakultas Psikologi Universitas Indonesia.Argue, A., Johnson, D. and White, L. (1999). Age and Religiosity: Evidence froma Three-WavePanel Analysis. 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University of Southern CaliforniaClements, R. and Swensen, C. (2000). Commitment to one’s spouse as a predictorof maritalquality among older couples. Curr Psychol, 19(2), pp.110-119.DeGenova, M. and Rice, F. (2005). Intimate relationships, marriages, andfamilies. New York:McGraw-Hill.DeGenova, M. (2008). Intimate relationships, marriages & families. Boston, MA: McGrawHill.Diener, E. (1984). Subjective well-being. Psychological Bulletin, 95(3), pp.542575.Duvall, E. and Miller, B. (1985). Marriage and family development. New York: Harper & Row.Fowers, B. and Olson, D. (1993). ENRICH Marital Satisfaction Scale: A brief research andclinical tool. Journal of Family Psychology, 7(2), pp.176-185.Gravetter, F.J. & Forzano, L.B. (2009). Research methods for the behavioral sciences (Edisike-3). Belmont, CA: Wadsworth.Goltz, J.W. (1987). Correlates in marital commitment. Disertasi Doktoral.Kanada: UniversitasAlberta.Hansen, D., Kelley, H. and Thibaut, J. (1982). Interpersonal Relations: A Theory ofInterdependence. Journal of Marriage and the Family, 44(1), p.246.Hassan, R. (2007). On Being Religious: Patterns of Religious Commitment in MuslimSocieties. The Muslim World, 97(3), pp.437-478.Hatch, R., James, D. and Schumm, W. (1986). Spiritual Intimacy and MaritalSatisfaction. Family Relations, 35(4), p.539.Harris, S. S. (2005). Marital commitment and religiosity in a sample of adults in Utah. AllGraduate Theses and Dissertations. Paper 2851. http://digitalcommons.usu.edu/etd/2851Haseley, J. and Riggs, S. (2006). Marital satisfaction among newly married couples. Denton:University of North Texas.Hawkins, M. (1981). Care of the dying. BMJ, 282(6280), pp.1969-1969.Heaton, T. and Albrecht, S. (1991). Stable Unhappy Marriages. Journal of Marriage and theFamily, 53(3), p.747.Heaton, T., Albrecht, S. and Martin, T. (1985). The Timing of Divorce. Journal of Marriageand the Family, 47(3), p.631.Hoge, D. and Hoge, J. (1984). Period Effects and Specific Age Effects Influencing Values ofAlumni in the Decade after College. Social Forces, 62(4), p.941Impett, E., Beals, K. and Peplau, L. (2001). Testing the investment model of relationshipcommitment and stability in a longitudinal study of married couples. Curr Psychol, 20(4),pp.312-326.Johnson, M. P. (1973). Commitment: A conceptual structure and empirical application. TheSociological Quarterly, 14(3), 395-406.

Background:SpA has historically been a difficult clinical diagnosis, especially early diagnosis. Two imaging techniques that address this problem are magnetic resonance imaging (MRI) and Single-Photon Emission Computed Tomography-Computed Tomography (SPECT-CT). Their accuracies have not been adequately compared.Objectives:The purpose of this study is to compare the sensitivities and specificities of SPECT-CT and MRI in SpA.Methods:This retrospective study assessed all patients who underwent SPECT-CT of the sacroiliac joint to assess for SpA. The results of SPECT-CT were compared against MRI for all patients in the cohort who underwent an MRI within 4 weeks of the SPECT-CT. A diagnosis of SpA in the discharge summary was considered the reference standard, and was based on a combination of clinical scenario, response to therapy, imaging,, patient history or lab index.Results:200 patients (173 men; average 22±4 years of age) were included SpA was diagnosed in 189 (AS patients=99 and excluded in 11. SPECT-CT and MRI had similar (P >0 .05;k ¼ 0.74) sensitivities (0.94 vs 0.94),specificities (1.00 vs 1.00),positive predictive values (1.00 vs 1.00),negative predictive values (0.94 vs 0.80),and accuracies (0.97 vs 0.95) when compared to the reference standard.Conclusion:Although MRI remains the initial modality of choice in early diagnosing SpA, SPECT-CT appears diagnostically equivalent and should be considered a viable supplementary or alternative imaging modality particularly if there is contra-indication or inaccessibility to MRI.References:[1]Taurog J D, Chhabra A, Colbert R A. Ankylosing Spondylitis and Axial Spondyloarthritis[J]. N Engl J Med, 2016,375(13):1303.[2]van der Linden S, Valkenburg H A, Cats A. Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria[J]. Arthritis Rheum, 1984,27(4):361-368.[3]Ward M M, Deodhar A, Gensler L S, et al. 2019 Update of the American College of Rheumatology/Spondylitis Association of America/Spondyloarthritis Research and Treatment Network Recommendations for the Treatment of Ankylosing Spondylitis and Nonradiographic Axial Spondyloarthritis[J]. Arthritis Rheumatol, 2019,71(10):1599-1613.[4]Boonen A, Sieper J, van der Heijde D, et al. The burden of non-radiographic axial spondyloarthritis[J]. Semin Arthritis Rheum, 2015,44(5):556-562.[5]Sieper J, Rudwaleit M, Baraliakos X, et al. The Assessment of SpondyloArthritis international Society (ASAS) handbook: a guide to assess spondyloarthritis[J]. Ann Rheum Dis, 2009,68 Suppl 2:i1-i44.[6]Bermo M, Behnia S, Fair J, et al. Review of Extraskeletal Activity on Tc-99m Methylene Diphosphonate Bone Scintigraphy and Value of Cross-Sectional and SPECT-CT Imaging Correlation[J]. Curr Probl Diagn Radiol, 2018,47(5):324-332.[7]Ward M M, Deodhar A, Akl E A, et al. American College of Rheumatology/Spondylitis Association of America/Spondyloarthritis Research and Treatment Network 2015 Recommendations for the Treatment of Ankylosing Spondylitis and Nonradiographic Axial Spondyloarthritis[J]. Arthritis Rheumatol, 2016,68(2):282-298.[8]Abdelhafez Y G, Hagge R J, Badawi R D, et al. Early and Delayed 99mTc-MDP SPECT/CT Findings in Rheumatoid Arthritis and Osteoarthritis[J]. Clin Nucl Med, 2017,42(11):e480-e481.Figure 1.An 20-years-old man with 5 years of low back pain and spine malformation. (A) SPECT-CT showed an abnormal concentration of radioactivity in SIJ. (B–C) In SIJ, MRI showed a high signal on T1-WI, and a high signal on STIR.Figure 2.An 37-year-old man with 20 years of low back pain and spine malformation. (A) SPECT-CT showed an abnormal concentration of radioactivity in SIJ. (B–C) In SIJ, MRI showed a high signal on T1-WI, and a low signal on STIR.Disclosure of Interests:Qing Han: None declared, Zhaohui Zheng: None declared, Kui Zhang: None declared, Zheng Yu: None declared, Fengfan Yang: None declared, Qiang Liang: None declared, Ping Zhu: None declared, Xenofon Baraliakos Grant/research support from: Grant/research support from: AbbVie, BMS, Celgene, Chugai, Merck, Novartis, Pfizer, UCB and Werfen, Consultant of: AbbVie, BMS, Celgene, Chugai, Merck, Novartis, Pfizer, UCB and Werfen, Speakers bureau: AbbVie, BMS, Celgene, Chugai, Merck, Novartis, Pfizer, UCB and Werfen

We have performed optical investigations of layers formed by sheets of aligned multi-wall carbon nanotubes (MWCNTs). Nanotubes have extraordinary mechanical, electrical and optical properties when they are unidirectionally oriented. Free-standing CNT sheets drawn mechanically from vertically-grown MWCNT forests can be deposited on glass substrates, with nanotube bundles mostly oriented in the drawing direction. These CNT sheet layers have optical anisotropy, which can be detectable even for a single CNT sheet. We have studied the optical anisotropy in transmission, finding a similar value at different length scales indicating similarities in the orientational order of the nanotubes, thus preservation of their average degree of order. Finally, light transmission was observed when the samples were at 45 deg between crossed polarizers. Full Text: PDF ReferencesS. Iijima, "Helical microtubules of graphitic carbon", Nature 354, 56 (1991). CrossRef S. Iijima, "Structural flexibility of carbon nanotubes", J. Chem. Phys. 104, 2089 (1996). CrossRef T.K. Truong et al., "Multifunctional characterization of carbon nanotube sheets, yarns, and their composites", Curr. Appl. Phys. 16, 1250 (2016). CrossRef L. Ren et al., "Carbon Nanotube Terahertz Polarizer", Nano Lett. 9, 7 (2009). CrossRef J. Kyoung et al., "A Reel-Wound Carbon Nanotube Polarizer for Terahertz Frequencies", Nano Lett. 11, 10 (2011). CrossRef M. Zhang et al., "Strong, Transparent, Multifunctional, Carbon Nanotube Sheets", Science 309, 5738 (2005). CrossRef K. Jiang, Q. Li, S. Fan, "Nanotechnology: Spinning continuous carbon nanotube yarns", Nature 419, 801 (2002). CrossRef

ABSTRACT Cas-Br-E and Graffi are two murine viruses that induce myeloid leukemia in mice: while Cas-Br-E induces mostly non-T, non-B leukemia composed of very immature cells, Graffi causes exclusively a granulocytic leukemia (E. Rassart, J. Houde, C. Denicourt, M. Ru, C. Barat, E. Edouard, L. Poliquin, and D. Bergeron, Curr. Top. Microbiol. Immunol. 211:201–210, 1995). In an attempt to understand the basis of the myeloid specificity of these two retroviruses, we used DNase I footprinting analysis and gel mobility shift assays to identify a number of protein binding sites within the Cas-Br-E and Graffi U3 regions. Two protected regions include potential GATA binding sites. Methylation interference analysis with different hematopoietic nuclear extracts showed the importance of the G residues in these GATA sites, and supershift assays clearly identified the binding factors as GATA-1, GATA-2, and GATA-3. Transient assays with long terminal repeat (LTR)-chloramphenicol acetyltransferase constructs showed that these three GATA family members are indeed able to transactivate Cas-Br-E and Graffi LTRs. Thus, the availability and relative abundance of the various members of the GATA family of transcription factors in a given cell type could influence the transcriptional tissue specificity of murine leukemia viruses and hence their disease specificity.

Antifeedant activity of neem leaf (A. indica A. Juss) has been identified by against Tenebrio molitor bio-indicator. The highest activity was obtained on ethyl acetate extract at 0.5% concentration having Antifeedant Index (AI) of 51.53% and most active at 10% concentration of 82.05%. The method used to test the antifeedant activity is the no choice leaf disk method. Secondary metabolites contained in neem leaf extract (A. indica A. juss) include terpenoids, steroids, flavonoids, saponins and phenolics. Keywords: Neem leaf (Azadirachta Indica A.Juss), No choice leaf disk method, Antifeedant ActivityREFFERENCES Benge, M.D. 1986. Neem the Cornucopia Tree. S and T/FENR Agroforestation Technical Series No. 5. Agency for International Development Washington, D.C.190p.Schumutterer., H. 2002. Properties and Potensial of Natural pPsticides from Neem Tree, Azadirachta indica Ann. Rev. Entomol. 35; 271-291Alzohairy, M.A. 2016. Review Article Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment, Article ID 7382506, 11p.4. Patel, S.M., Venkata., K.C.N., Bhattacharyya, P., Sethi, G., Bishayee, A. 2016. Potential of Neem (Azadirachta indica) For Prevention and treatment of Oncologic Diseases Seminar In Cancer BiologyDiabate, D., Gnago, J.A., Tano, Y., 2014. Toxicity, Antifeedant and Repellent, effect of Azadirachta indica A. Juss and Jatropa carcus L. aqueous extracts agaianst Plutella xylostella (Lepidoptera: Plutellidae) J. Basic. Appl. Sci. Res. 4 (11) : 51 – 60Jeyasankar, A., and Gokilamani, D., 2016. Biology and eco-friendly control of Amaranth pests, Hymenia recurvalis Fabricus and Psara basalis (Lepidoptera : Crambidae) Inter. J. Acad. Stud. 2 (4): 218 – 230.Pavunraj, M., Muthu, C., Ignacimuthu,S., Janarthanan, S., Duraipandiyan, V., Raja, N. and Vimalraj, S. 2011. Antifeedant Activity of a Novel 6-(4,7-hydroxyl-heptyl)Quinone From The Leaves of The Milkweed Pergularia daemia on The Cotton Bollworm Helicoverpa armegera (Hub.) and The Tobacco Armworm Spodoptera litura (Fab.) Phytoparasitica 39 : 145 – 150.Munoz, E., Lamilla, C., Marin, J.C., Alarcon, J., Cespedes, C.L.m 2012. Antifeedant, Insect Growth and Insecticidal Effec of Calceolaria talcana (Calceolariaceae) on Dropsopphila melanogaster and Spodoptera frugiperda Industrial Crops and Product, 42, 137 – 144.Saxena, M., Saxena, J., Nema, R., Singh, D and Gupta, A. 2013. Phytochemistry of Medicinal Plants Journal pharmacognosy and Phytochemistry, 1;6.Liu, L., Zhao, Y.L., Cheng, G.G., Chen, Y.Y., Qin, angX.J., C.C.W., Yang, X.W., Liu, Y.P., Luo, X.D, 2014. Antifeedant activity and effect of fruits and seed extract of Cabralea canjerana canjerana (Vell.) Mart. (Meliaceae) on the immature stages of the fall armworm Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae Crops and Product. 65 ; 156 – 158.Schoonhven, LM. 1982. Biologycal Aspect of Antifeedant . Ent, Exp and Appl .31: 57 - 69Gahukar, R.T. 2014. Factor Affecting Content and Bioefficacy of Neem (Azadirachta indica A. Juss) Phytochemicals used in Agriculture pest control A Review. Crop Protection. 62: 93 – 99.Pattanaik, S.J., Ranghupati, N.D.,Chary, P.2006.Ecomorphometric Marker Reflect Variation in Azadirachtin Contents of Azadirachta indica A. Juss. (Meliaceae) in Select Regions of Andhra Pradesh India. Curr. Sci, 91:628 – 636.Adel, M.M., Sehnal, H., Jurzysta, M. 2000. Effects of Alfalfa safonin on the Moth Spodoptera litura Journal of Chemical Ecology, 26 : 7-14Shuklar, Y.N., Rani, A. Tripathi,A.K., Sharma, S. 1996. Antifeedant, Activity of Ursolic Acid Isolation from Duboisia myoporoides Phytotheraphy, 10 : 359 – 360Walter, J.F. 1999. Commercial With Neem Product, P. 155-170. In Franklin R, Hall and Julius J. Menn. Biopesticides Use and Delivery. Humana Press. Totowo, New Jersey.

Oleander (Nerium oleander L.) shrubs presenting mottling, leaf tip and margin scorch, short internodes, defoliation, and branch dieback were observed at different localities in the Central Valley in Costa Rica. Severity of the symptoms ranged widely, and most plants showed both diseased and healthy branches. In severe cases, entire sections of the plant were defoliated. Symptoms resembled those described for oleander leaf scorch (OLS) caused by the bacterium Xylella fastidiosa in the United States (3). This bacterium has been reported in coffee and citrus plants in Costa Rica. Sixty plants from five different places were sampled and tested using ELISA (Agdia Inc., Elkhart, IN) against X. fastidiosa. Thirty-five plants showed absorbance mean value of duplicate wells greater than the mean of control wells plus three times the standard deviation, and therefore were considered positive. Thirty-three of the sixty samples were processed for an immunofluorescence assay modified from Carbajal et al. (1) with antibody to X. fastidiosa (Agdia Inc.). Thirteen samples showed fluorescent rod-shaped bacilli with morphology similar to those observed from a pure culture of X. fastidiosa obtained from coffee. Ten of these thirteen samples were positive by ELISA. DNA extracts (2) from three of the oleander plants with high ELISA absorbance values were tested by nested PCR with primer pair 272-1/272-2 followed by the pair 272-1 int/272-2 int (4). Two of the samples were positive for the bacterium and one of the PCR products was cloned and sequenced in both directions (GenBank Accession No. EU009615). The negative (PCR mix) and positive (pure culture of X. fastidiosa isolated from grapevine) controls for nested-PCR were indeed negative and positive, respectively. The BLAST program was used to compare the sequence to the nucleotide collection (nr/nt) and Microbe Assembled Genomes databases in GenBank. All matches corresponded to X. fastidiosa sequences. The sequence showed 97% similarity with strains Found-4 (coffee strain from Brazil) and Found-5 (citrus strain from Brazil) and 96% similarity with strain Ann-1 from oleander in California. On the basis of serological, microscopic, and molecular detection of X. fastidiosa from oleander exhibiting symptoms of OLS similar to those reported in the literature, this pathogen likely is causing the symptoms we observed in Costa Rica. References: (1) D. Carbajal et al. Curr. Microbiol. 49:372, 2004. (2) M. J. Green et al. Plant Dis. 83:482, 1999. (3) Q. Huang et al. Plant Dis. 88:1049, 2004. (4) M. R. Pooler and J. S. Hartung. Curr. Microbiol. 31:377, 1995.

Introdução: A candidemia é uma das infecções nasocomiais mais frequentes a nível mundial e apresenta diferentes espécies de Candida envolvidas com o desenvolvimento dessa patologia. Objetivo: Este estudo analisou os aspectos epidemiológicos e as diferentes espécies de Candida associadas à candidemia. Material e Métodos: Uma revisão de literatura foi realizada, através da leitura de artigos científicos publicados nas bases de dados Pubmed e Scielo nos últimos cinco anos. Utilizou-se as seguintes combinações de descritores: Candidemia, Candidemia e Prevalência, Candidemia e Incidência, Candidemia e Candida. Resultados: A candidemia é a infecção fúngica mais hostil atualmente encontrada e prevalente nas populações estudadas. A população mais afetada são os recém-nascidos. Apresenta altas taxas de mortalidade e disseminação em várias partes do mundo. A nutrição parenteral, a administração de antibióticos de amplo espectro, hospitalização prolongada, cirurgia prévia e colonização por Candida sp são os principais fatores de risco relatados na literatura. Múltiplas espécies fúngicas estão associadas a essa condição, porém C. albicans é a mais predominante, seguida por C. parapsilosis e C. tropicalis. Conclusões: A Candidemia é uma patologia prevalente e apresenta alta incidência e morbimortalidade nas populações estudadas das diferentes partes do mundo. C. albicans é a espécie mais associada, seguida por C. parapsilosis e C. tropicalis. Descritores: Candidemia; Prevalência; Incidência; Candida. Referências Treviño-Rangel RJ, Peña-López CD, Hernández-Rodríguez PA, Beltrán-Santiago D, González GM. Association between Candida biofilm-forming bloodstream isolates and the clinical evolution in patients with candidemia: An observational nine-year single center study in Mexico. Rev Iberoam Micol. 2018;35(1):11-16. Kaur H, Chakrabarti A. Strategies to Reduce Mortality in Adult and Neonatal Candidemia in Developing Countries. J Fungi (Basel). 2017;3(3):41. Wu PF, Liu WL, Hsieh MH, Hii IM, Lee YL, Lin YT et al. Epidemiology and antifungal susceptibility of candidemia isolates of non-albicans Candida species from cancer patients. Emerg Microbes Infect. 2017;6(10):e87. Vasilyeva NV, Raush ER, Rudneva MV, Bogomolova TS, Taraskina AE, Fang Y et al. Etiology of invasive candidosis agents in Russia: a multicenter epidemiological survey. Front Med. 2018;12(1):84-91. Barchiesi F, Orsetti E, Osimani P, Catassi C, Santelli F, Manso E. Factors related to outcome of bloodstream infections due to Candida parapsilosis complex. BMC Infect Dis. 2016;16:387. Barchiesi F, Orsetti E, Mazzanti S, Trave F, Salvi A, Nitti C, Manso E. Candidemia in the elderly: What does it change? PLoS One. 2017;12(5):e0176576. Benedict K, Roy M, Kabbani S, Anderson EJ, Farley MM, Harb S et al. Neonatal and pediatric candidemia: results from population-based active laboratory surveillance in four US locations, 2009-2015. J Pediatric Infect Dis Soc. 2018;7(3):e78-e85. Bhattacharjee P. Epidemiology and antifungal susceptibility of Candida species in a tertiary care hospital, Kolkata, India. Curr Med Mycol. 2016;2(2):20-7. Breda GL, Tuon FF, Meis JF, Herkert PF, Hagen F, de Oliveira LZ, Dias VC, da Cunha CA, Queiroz-Telles F. Breakthrough candidemia after the introduction of broad spectrum antifungal agents: A 5-year retrospective study. Med Mycol. 2018;56(4):406-15 Caggiano G, Lovero G, De Giglio O, Barbuti G, Montagna O, Laforgia N et al. Candidemia in the Neonatal Intensive Care Unit: a retrospective, observational survey and analysis of literature Data. Biomed Res Int. 2017;2017:7901763. Fu J, Ding Y, Wei B, Wang L, Xu S, Qin P, Wei L, Jiang L. Epidemiology of Candida albicans and non-C.albicans of neonatal candidemia at a tertiary care hospital in western China. BMC Infect Dis. 2017;17(1):329. Guzzetti LB, Vescina CM, Gil MF, Gatti BM. Candidemias en pediatría: distribución de especies y sensibilidad a los antifúngicos [Candidemia in Pediatrics: Species distribution and antifungal susceptibility]. Rev Argent Microbiol. 2017;49(4):320-22. Kofteridis DP, Valachis A, Dimopoulou D, Andrianaki AM, Christidou A, Maraki S, Spernovasilis NA, Samonis G. Factors Influencing Non-albicans Candidemia: A Case-Case-Control Study. 2017; 182(7-8):665-72. Kubiak DW, Farmakiotis D, Arons V, Hollins RM, Rostas SE, Weiser LM et al. Utility of in-house fluconazole disk diffusion susceptibility testing in the treatment of candidemia. Diagn Microbiol Infect Dis. 2016;84(3):223-26. Li D, Zhang W, Zheng S, Ma Z, Zhang P, Liu Z. Surveillance study of candidemia in cancer patients in North China. Med Mycol. 2013;51(4):378-84. Li D, Xia R, Zhang Q, Bai C, Li Z, Zhang P. Evaluation of candidemia in epidemiology and risk factors among cancer patients in a cancer center of China: an 8-year case-control study. BMC Infect Dis. 2017;17(1):536. Lortholary O, Renaudat C, Sitbon K, Desnos-Ollivier M, Bretagne S, Dromer F; French Mycoses Study Group. The risk and clinical outcome of candidemia depending on underlying Intensive Care Med. 2017; 43(5):652-62. Lovero G, De Giglio O, Montagna O, Diella G, Divenuto F, Lopuzzo M, Rutigliano S, Laforgia N, Caggiano G, Montagna MT. Epidemiology of candidemia in neonatal intensive care units: a persistent public health problem. Ann Ig. 2016;28(4):282-87. Márquez F, Iturrieta I, Calvo M, Urrutia M, Godoy-Martínez P. Epidemiología y susceptibilidad antifúngica de especies causantes de candidemia en la ciudad de Valdivia, Chile [Epidemiology and antifungal susceptibility of species producing candidemia in Valdivia, Chile]. Rev Chilena Infectol. 2017;34(5):441-46. Pinhati HM, Casulari LA, Souza AC, Siqueira RA, Damasceno CM, Colombo AL. Outbreak of candidemia caused by fluconazole resistant Candida parapsilosis strains in an intensive care unit. BMC Infect Dis. 2016;16(1):433. Siri L, Legarraga P, García P, González T, Rabagliati R. Cambios clínicos y epidemiológicos de candidemias en pacientes adultos desde 2000 a 2013. Rev Chilena Infectol. 2017;34(1):19-26. Spiers R, Smyth B, Lamagni T, Rooney P, Dorgan E, Wyatt T et al. The epidemiology and management of candidemia in Northern Ireland during 2002-2011, including a 12-month enhanced case review. Med Mycol. 2019;57(1):23-9. Tiraboschi IN, Pozzi NC, Farías L, García S, Fernández NB. Epidemiología, especies, resistencia antifúngica y evolución de las candidemias en un hospital universitario de Buenos Aires, Argentina, durante 16 años [Epidemiology, species, antifungal resistance and outcome of candidemia in a university hospital in Buenos Aires, Argentina for 16 years]. Rev Chilena Infectol. 2017;34(5):431-40. Vena A, Bouza E, Valerio M, Padilla B, Paño-Pardo JR, Fernández-Ruiz M et al. Candidemia in non-ICU surgical wards: comparison with medical wards. PLoS One. 2017;12(10):e0185339. Kelly MS, Benjamin DK Jr, Smith PB. The epidemiology and diagnosis of invasive candidiasis among premature infants. Clin Perinatol. 2015;42(1):105-17, viii-ix. Wu JQ, Zhu LP, Ou XT, Xu B, Hu XP, Wang X et al. Epidemiology and risk factors for non-Candida albicans candidemia in non-neutropenic patients at a Chinese teaching hospital. Med Mycol. 2011;49(5):552-55. Navalkele BD, Revankar S, Chandrasekar P. Candida auris: a worrisome, globally emerging pathogen. Expert Rev Anti Infect Ther. 2017;15(9):819-27. Spivak ES, Hanson KE. Candida auris: an Emerging Fungal Pathogen. J Clin Microbiol. 2018;56(2):e01588-17. Colombo AL, Guimarães T, Sukienik T, Pasqualotto AC, Andreotti R, Queiroz-Telles F et al. Prognostic factors and historical trends in the epidemiology of candidemia in critically ill patients: an analysis of five multicenter studies sequentially conducted over a 9-year period Intensive Care Med. 2014;40(10):1489-98. Gehring GM, Carrilho CMM, Pelisson M, Perugini M, Tano ZN. Candidemia: Revisão Bibliográfica. J Infect Control. 2015;4(4):1-19. Lepak A, Andes D. Fungal sepsis: optimizing antifungal therapy in the critical care setting. Crit Care Clin. 2011;27(1):123-47. Pappas PG, Kauffman CA, Andes DR, Clancy CJ, Marr KA, Ostrosky-Zeichner L et al. Clinical Practice Guideline for the Management of Candidiasis: 2016 Update by the Infectious Diseases Society of America. Clin Infect Dis. 2016;62(4):e1-50. Wisplinghoff H, Ebbers J, Geurtz L, Stefanik D, Major Y, Edmond MB et al. Nosocomial bloodstream infections due to Candida spp. in the USA: species distribution, clinical features and antifungal susceptibilities. Int J Antimicrob Agents. 2014;43(1):78-81.

Small GTPases of the rab family are involved in the regulation of vesicular transport. The restricted distribution of each of these proteins in mammalian cells has led to the suggestion that different rab proteins act at different steps of transport (Pryer, N. K., L. J. Wuestehube, and R. Sheckman. 1992. Annu Rev. Biochem. 61:471-516; Zerial, M., and H. Stenmark. 1993. Curr. Opin. Cell Biol. 5:613-620). However, in this report we show that the Ypt1-GTPase, a member of the rab family, is essential for more than one step of the yeast secretory pathway. We determined the secretory defect conferred by a novel ypt1 mutation by comparing the processing of several transported glycoproteins in wild-type and mutant cells. The ypt1-A136D mutant has a change in an amino acid that is conserved among rab GTPases. This mutation leads to a rapid and tight secretory block upon a shift to the restrictive temperature, and allows for the identification of the specific steps in the secretory pathway that directly require Ypt1 protein (Ypt1p). The ypt1-A136D mutant exhibits tight blocks in two secretory steps, ER to cis-Golgi and cis- to medial-Golgi, but later steps are unaffected. Thus, it is unlikely that Ypt1p functions as the sole determinant of fusion specificity. Our results are more consistent with a role for Ypt1/rab proteins in determining the directionality or fidelity of protein sorting.

The tumor suppressor p53 is important in the decision to either arrest cell cycle progression or induce apoptosis in response to a variety of stimuli. p53 posttranslational modifications and association with other proteins have been implicated in the regulation of its stability and transactivation activity. Here we show that p53 is phosphorylated by the mitotic kinase Aurora-A at serine 215. Unlike most identified phosphorylation sites of p53 that positively associate with p53 function (Brooks, C. L., and Gu, W. (2003)Curr. Opin. Cell Biol.15, 164–171), the phosphorylation of p53 by Aurora-A at Ser-215 abrogates p53 DNA binding and transactivation activity. Downstream target genes of p53, such asp21Cip/WAF1andPTEN, were inhibited by Aurora-A in a Ser-215 phosphorylation-dependent manner (i.e.phosphomimic p53-S215D lost and non-phosphorylatable p53-S215A retained normal p53 function). As a result, Aurora-A overrides the apoptosis and cell cycle arrest induced by cisplatin and γ-irradiation, respectively. However, the effect of Aurora-A on p53 DNA binding and transactivation activity was not affected by phosphorylation of Ser-315, a recently identified Aurora-A phosphorylation site of p53 (Katayama, H., Sasai, K., Kawai, H., Yuan, Z. M., Bondaruk, J., Suzuki, F., Fujii, S., Arlinghaus, R. B., Czerniak, B. A., and Sen, S. (2004)Nat. Genet.36, 55–62). Our data indicate that phosphorylation of p53 at Ser-215 by Aurora-A is a major mechanism to inactivate p53 and can provide a molecular insight for Aurora-A function.

Citrus variegated chlorosis (CVC) is an important disease mainly of sweet orange (Citrus sinensis (L.) Osbeck) cultivars. It was first described in Brazil in the state of Sā Paulo in 1987 (4). The disease has spread to all Brazilian states that grow citrus and is affecting more than one-third of the orange trees grown in Brazil. CVC is caused by Xylella fastidiousa, a xylem-limited, gram-negative bacterium. During the last 4 years, symptoms including leaf interveinal chlorosis, stunting, canopy dieback, and hard and undersized fruits, similar to those caused by CVC (3), appeared in sweet orange trees used as shade plants for coffee plantations and as fence posts in Costa Rica. Necrotic lesions on the abaxial side of the leaves as reported in Brazil were rarely observed. Leaf petiole samples from 25 symptomatic sweet orange trees reacted positively with a X fastidiosa-specific antiserum (AGDIA Inc., Elkart, IN) in a double-sandwich antibody enzyme-linked immunosorbent assay (DAS-ELISA). A fastidious, gram-negative bacterium identified as X. fastidiosa using DAS-ELISA was isolated on perwinkle wilt (PW) medium plates (1) from citrus stems showing CVC symptoms, but not from asymptomatic trees. The isolated colonies were circular and opalescent with diameters of 2 to 3 mm and were clearly visible within 6 to 7 days after streaking. Petiole sections from symptomatic plants observed with scanning electron microscopy showed rod-shaped bacteria with rippled cell walls tightly packed in xylem vessels, as described for X. fastidiosa previously (2), and with transmission electron microscopy, the bacteria were morphologically similar to those reported previously for CVC (2). To our knowledge, this is the first report of X. fastidiosa associated with citrus in Costa Rica. References: (1) M. J. Davis et al. Curr. Microbiol. 6:309, 1981. (2) J. S. Hartung et al. Phytopathology 84:591, 1994. (3) R. F. Lee et al. Summa Phytopathol. 19:123, 1993. (4) V. Rossetti et al. 1990, C.R. Acad. Sci. (Paris) 310:345–349.

The concept of plants and microbes utilization for remediation measure of pollutant contaminated soil is the newest development in term of petroleum waste management technique. The research objective was to obtain wild grass types and hydrocarbonoclastic bacteria which are capable to synergize in decreasing petroleum concentration within petroleum contaminated soil. This research was conducted by using randomized completely block design. This research was conducted by using randomized completely block design. The first factor treatments were consisted of without plant, Tridax procumbens grass and Lepironia mucronata grass. The second factor treatments were consisted of without bacterium, single bacterium of Alcaligenes faecalis, single bacterium of Pseudomonas alcaligenes, and mixed bacteria of Alcaligenes faecalis with P. alcaligenes. The results showed that mixed bacteria (A. faecalis and P. alcaligenes) were capable to increase the crown and roots dry weights of these two grasses, bacteria population, percentage of TPH (total petroleum hydrocarbon) decrease as well as TPH decrease and better pH value than that of single bacterium. The highest TPH decrease with magnitude of 70.1% was obtained on treatment of L. mucronata grass in combination with mixed bacteria.[How to Cite: Gofar N. 2013.Synergism of Wild Grass and Hydrocarbonoclastic Bacteria in Petroleum Biodegradation. J Trop Soils 18 (2): 161-168. Doi: 10.5400/jts.2013.18.2.161][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.2.161]REFERENCESBello YM. 2007. Biodegradation of Lagoma crude oil using pig dung. Afr J Biotechnol 6: 2821-2825.Gerhardt KE, XD Huang, BR Glick and BM Greenberg. 2009. Phytoremediation and rhizoremediation of organic soil contaminants: Potential and challenges. Plant Sci 176: 20-30.Glick BR. 2010. Using soil bacteria to facilitate phytoremediation. Biotechnol Adv 28: 367-374. Gofar N. 2011. Characterization of petroleum hydrocarbon decomposing fungi isolated from mangrove rhizosphere. J Trop Soils 16(1): 39-45. doi: 10.5400/jts.2011.16.1.39Gofar N. 2012. Aplikasi isolat bakteri hidrokarbonoklastik asal rhizosfer mangrove pada tanah tercemar minyak bumi. J Lahan Suboptimal 1: 123-129 (in Indonesian). Hong WF, IJ Farmayan, CY Dortch, SK Chiang and JL Schnoor. 2001. Environ Sci Technol 35: 1231.Khashayar T and T Mahsa. 2010. Biodegradation potential of petroleum hydrocarbons by bacterial diversity in soil. Morld App Sci J 8: 750-755.Lal B and S Khanna. 1996. Degradation of Crude Oil by Acinetobacter calcoaceticus and Alcaligenes odorans, J Appl Bacteriol 81: 355- 362.Mackova M, D Dowling and T Macek. 2006. Phytoremediation and rhizoremediation: Theoretical background. Springer, Dordrecht, Netherlands. 300 p. Malik ZA and S Ahmed. 2012. Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium. Afr J Biotechnol 11: 650-658.Mendez MO and RM Maier. 2008. Phytostabilization of mine tailings in arid and semiarid environment an emerging remediation technology. Environ Health Prospect 116: 278-283.Milic JS, VP Beskoski, MV Ilic, SM Ali, GDJ Cvijovic and MM Vrvic. 2009. Bioremediation of soil heavily contaminated with crude oil and its products: composition of the microbial consortium. J Serb Chem Soc 74: 455-460.Mukre AM, AA Hamid, A Hamzah and WM Yusoff. 2008. Development of three bacteria consortium for the bioremediation of crude petroleum-oil in contaminated water. J Biol Sci 8: 73-79.Ndimele PE. 2010. A review on the phytioremediation of petroleum hydrocarbon. Pakistan J Biol Sci 12: 715-722.Newman LA and CM Reynolds. 2004. Phytoremediation of organic compounds. Curr Opin Biotechnol 15: 225-230.Onwuka F, N Nwachoko, and E Anosike. 2012. Determination of total petroleum hydrocarbon (TPH) and some cations (Na+, Ca2+ and Mg2+) in a crude oil polluted soil and possible phytoremediation by Cynodon dactylon L (Bermuda grass). J Environ Earth Sci 2: 12-17.Pezeshki SR, MW Hester, Q Lin and JA Nyman. 2000. The effect of oil spill and clean-up on dominant US Gulf Coast Marsh Macrophytes: a review. Environ Pollution 108: 129-139.Pikoli MR, P Aditiawati and DI Astuti. 2000. Isolasi bertahap dan identifikasi isolat bakteri termofilik pendegradasi minyak bumi dari sumur bangko. Laporan Penelitian pada Jurusan Biologi, ITB, Bandung (unpublished, in Indonesian).Pilon-Smits E and JL Freeman. 2006. Environmental cleanup using plants: biotechnological advances and ecological considerations. Front Ecol Environ 4: 203-10. Rahman KSM, JT Rahman, P Lakshmanaperumalsamy, and IM Banat. 2002. Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresource Technol 85: 257-261.Rossiana N. 2004. Oily Sludge Bioremediation with Zeolite and Microorganism and It’s Test with Albizia Plant (Paraserianthes falcataria) L (Nielsen). Laboratory of Environmental Microbiology, Department of Biology Padjadjaran University, Bandung (unpublished).Rossiana, N. 2005. Penurunan Kandungan Logam Berat dan Pertumbuhan Tanaman Sengon (Paraserianthes falcataria L (Nielsen) Bermikoriza dalam Media Limbah Lumpur Minyak Hasil Ekstraksi. Laboratorium Mikrobiologi dan Biologi Lingkungan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjajaran, Bandung (in Indonesian).Sathishkumar M, B Arthur Raj, B Sang-Ho, and Y Sei-Eok. 2008. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas clean. Ind J Biotechnol 36: 92-96.Shirdam R, AD Zand, GN Bidhendi and N Mehrdadi. 2008. Phytoremediation of hydrocarbon-contaminated soils with emphasis on effect of petroleum hydrocarbons on the growth of plant species. Phytoprotection 89: 21-29.Singer AC, DE Crowley and IP Thompson. 2003. Secondary plant metabolites in phytoremediation and biotransformation. Trends Biotechnol 21: 123-130.Singh A and OP Ward. 2004. Applied Bioremediation and Phytoremediation. Springler, Berlin, 281p.Surtikanti H and W Surakusumah. 2004. Peranan Tanaman dalam Proses Bioremediasi Oli Bekas dalam Tanah Tercemar. Ekol Biodivers Trop 2: 48-52 (in Indonesian).Wenzel WW. 2009. Rhizosphere processes and management in plant-assisted bioremediation (phytoremediation) of soil. Plant Soil 321: 385-408.Widjajanti H, I Anas, N Gofar and MR Ridho. 2010. Screening of petroleum hydrocarbons degrading bacteria as a bioremediating agents from mangrove areas. Proceeding of International Seminar, workshop on integrated lowland development and management, pp. C7 1-9.Widjajanti H. 2012. 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Mammalian cells regulate tubulin mRNA abundance by a posttranscriptional mechanism dependent on the concentration of tubulin monomer. Treatment of mammalian cells with microtubule-depolymerizing drugs and microtubule-polymerizing drugs causes decreases and increases in tubulin mRNA, respectively (D. W. Cleveland, Curr. Opin. Cell Biol. 1:10-14, 1989). In striking contrast to the case with mammalian cells, perturbation of microtubules in Tetrahymena thermophila by microtubule-depolymerizing or -polymerizing drugs increases the level of the single alpha-tubulin gene message by increasing transcription (L. A. Stargell, D. P. Heruth, J. Gaertig, and M. A. Gorovsky, Mol. Cell. Biol. 12:1443-1450, 1992). In this report we show that antimicrotubule drugs preferentially induce the expression of one of two beta-tubulin genes (BTU1) in T. thermophila. In contrast, deciliation induces expression of both beta-tubulin genes. Tubulin gene expression was examined in a mutant strain created by transformation with an in vitro-mutagenized beta-tubulin gene that conferred resistance to microtubule-depolymerizing drugs and sensitivity to the polymerizing drug taxol and in a strain containing a nitrosoguanidine-induced mutation in the single alpha-tubulin gene that conferred the same pattern of drug sensitivities. In both cases the levels of tubulin mRNA expression from the drug-inducible BTU1 gene in the mutant cells paralleled the altered growth sensitivities to microtubule drugs. These studies demonstrate that T. thermophila has distinct, gene-specific mechanisms for modulating tubulin gene expression depending on whether ciliary or cytoplasmic microtubules are involved. They also show that the cytoplasmic microtubule cytoskeleton itself participates in a signal transduction pathway that regulates specific tubulin gene transcription in T. thermophila.

ABSTRACTSalmonellosis is one of the most common causes of food-borne disease in the United States. Increasing antimicrobial resistance and corresponding increases in virulence present serious challenges. Currently, empirical therapy for invasiveSalmonella entericainfection includes either ceftriaxone or ciprofloxacin (E. L. Hohmann, Clin. Infect. Dis. 32:263–269, 2001). TheblaCMY-2gene confers resistance to ceftriaxone, the antimicrobial of choice for pediatric patients with invasiveSalmonella entericainfections, making these infections especially dangerous (J. M. Whichard et al., Emerg. Infect. Dis. 11:1464–1466, 2005). We hypothesized thatblaCMY-2-positiveSalmonella entericawould exhibit increased MICs to multiple antimicrobial agents and increased resistance gene expression following exposure to ceftriaxone using a protocol that simulated a patient treatmentin vitro. SevenSalmonella entericastrains survived a simulated patient treatmentin vitroand, following treatment, exhibited a significantly increased ceftriaxone MIC. Not only would these isolates be less responsive to further ceftriaxone treatment, but because theblaCMY-2genes are commonly located on large, multidrug-resistant plasmids, increased expression of theblaCMY-2gene may be associated with increased expression of other drug resistance genes located on the plasmid (N. D. Hanson and C. C. Sanders, Curr. Pharm. Des. 5:881–894, 1999). The results of this study demonstrate that a simulated patient treatment with ceftriaxone can alter the expression of antimicrobial resistance genes, includingblaCMY-2andfloRinS. entericaserovar Typhimurium andS. entericaserovar Newport. Additionally, we have shown increased MICs following a simulated patient treatment with ceftriaxone for tetracycline, amikacin, ceftriaxone, and cefepime, all of which have resistance genes commonly located on CMY-2 plasmids. The increases in resistance observed are significant and may have a negative impact on both public health and antimicrobial resistance ofSalmonella enterica.

Mung bean (Vigna radiata (L.) R. Wilczek) is an important edible legume grown in Asia, particularly in the Indian subcontinent, where it is used for human and animal consumption. In September 2013, 10% of a group of 90 mung bean breeding lines in experimental plots of S. V. Agricultural College, Tirupati, Andhra Pradesh, India, exhibited symptoms typical of a phytoplasma infection, including stunting, extensive proliferation of branches, reduction in leaf size, phyllody, and longitudinal splitting of green pods followed by germination of green seeds producing small plants. These symptoms have been associated with mung bean phyllody (1,3). While the severity of infection varied within each line, on average, 20% of symptomatic plants did not produce seeds at all. Leaf samples from two symptomatic plants and one asymptomatic plant were collected and DNA was extracted from leaves following a CTAB DNA extraction procedure (2). Direct PCR and nested PCR assay was performed using phytoplasma 16S rRNA universal primer pairs P1/P7 and R16F2n/R16R2 (4). Both of the symptomatic samples produced 1.8 kb and 1.2 kb size amplicons after direct and nested PCR cycles, respectively. No amplicons were produced with DNA from the asymptomatic sample. Nested PCR products (1.2 kb) from the two symptomatic samples corresponding to the F2nR2 region of 16S rDNA were directly sequenced on an ABI 3730 XL automated sequencer at McLab sequencing services (McLab, CA). Both samples were 100% identical and the representative sequence was designated as APMBP and deposited in GenBank with the accession number KF811205. BLAST analysis revealed a 100% sequence identity with 16SrII group ‘Candidatus Phytoplasma aurantifolia’ phytoplasmas that include sesame phyllody phytoplasmas isolated from sesame and tomato in India (KF429485 and JX104335, respectively). Subgroup identification was performed using the iPhyClassifier online tool (5). The samples were identified as 16SrII-D subgroup phytoplasma based on their 100% identity to the reference strain (GenBank Accession No. Y10097) and virtual RFLP profiles. Phylogenetic analysis based on the F2nR2 sequences with the representative sequences were placed the APMBP in a single distinct cluster with the 16SrII-D reference strain Y10097. Although occurrence of phyllody on mung bean in India was first reported in 1988 (3), the report was based on the appearance of symptoms. This pathogen was recently reported as associated with mung bean phyllody in Pakistan (1). To our knowledge, this is the first report of ‘Ca. P. aurantifolia’ strain infecting mung bean in India. Phytoplasmas belonging to subgroup 16SrII-D are known to have a wide host range, including chickpea, peanuts, sesame, and tomato, which are commonly grown in this region. Mung bean plants infected early failed to produce normal seeds indicating of the potential of this 16SrII-D phytoplasma to become a production constraint for mung bean and other host crops in the area. References: (1) K. P. Akhtar et al. Plant Pathol. 59:399, 2010. (2) J. J. Doyle and J. L. Doyle. Phytochem. Bull. 19:11, 1987. (3) P. Lakshmanan et al. Curr. Sci. 57:809, 1988. (4) I. M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998. (5) Y. Zhao et al. Int. J. Syst. Evol. Microbiol. 59:2582, 2009.

Background:Psoriatic arthritis (PsA) is a chronic inflammatory disease associated with higher risk of cardiovascular events and metabolic syndrome. The inflammation not only accelerates atherosclerosis, but also may influence cardiovascular (CV) risk factors such as lipid profile, blood pressure and insulin resistance. Lipid profile has previously been studied in PsA, however this association is still controversial.Objectives:To study the frequency of altered lipid profile in patients with PsA and its association with disease activity.Methods:We studied all the patients with diagnosis of PsA who consecutively attended to Rheumatology Unit at Cordoba Hospital from July 2018 to December 2019. PsA was diagnosed according CASPAR criteria. Clinical and laboratory data were collected. The activity of the disease was evaluated by PASI, MDA and DAPSA. Quantitative variables will be expressed in median and 1st and 3rd interquartile; qualitative variables expressed in frequency and percentage. Correlation analysis was calculated using Spearman’s rank correlation coefficient. P<0.05 was considered statistically significant.Results:42 PsA patients were included. Mean age was 56 years old (47.25-62.75) and 54.76% were female (n=23). 92.86% (n=39) of the patients had plaque Psoriasis and 87.8% (n=36) had peripheral joint involvement.Frequency of comorbidities in PsA are shown in Graphic 1. 31 (73.8%) of the patients were treated with topical therapy, 3 (7.14%) with phototherapy, 31 (73.8%) with Methotrexate and 17 (41.46%) with biologics and JAK inhibitor. Activity Disease Index and Lipid profile are shown in Table 1 and 2.There was not association between Apo B/Apo A coefficient with DAPSA (rho=0.013; p=0.940) and MDA (rho=-0.029; p=0.867).Conclusion:In spite of the presence of cardiovascular factors in the majority of PsA patients, lipid profile is not correlated with disease activity in this population.References:[1]Ahlehoff O, Gislason GH, Charlot M, et al. Psoriasis is associated with clinically significant cardiovascular risk: A Danish nationwide cohort study. J Intern Med 2011;270:147-57.[2]Mallbris, L., Ritchlin, C.T., Ståhle, M. “Metabolic disorders in patients with psoriasis and psoriatic arthritis.” Curr RheumatolRep.8(5): 355–363. 2006[3]Ng CY, Tzeng I-S, Liu S-H, Chang Y-C, Huang Y-H. Metabolic parameters in psoriatic patients treated with interleukin-12/23 blockade (Ustekinumab). J Dermatol 2018; 45:309–313[4]Kaur S, Kingo K, Zilmer M. Psoriasis and cardiovascular risk – do promising new biomarkers have clinical impact? Mediators Inflamm 2017; 2017: 7279818[5]Gentile M, Peluso R, Di Minno MN, et al. Association between small dense LDL and sub-clinical atherosclerosis in patients with psoriatic arthritis. ClinRheumatol 2016; 35: 2023-9.Graphic 1.Comorbidities in PsATable. 1.Activity Disease Index in PsAACTIVITY INDEXn=42DAPSA14.45 (9.72-23.92)DAPSA≤4 REMISSION3>4 y ≤14 low disease activity16>14 y ≤28 moderate disease activity17>28 high disease activity3cDAPSA14.00 (8.00-23.00)/41*MDA9 (25)/36PASI2.20 (0.20-6.80)/41**Expressed in median and interquartiles.Qualitative variables expressed in frequency and percentage.Table. 2.Lipid Profile in PsA patients.Cholesterol (mg/dl)194.5 (164.8-218.2)HDL (mg/dl)48.00 (37.00-57.00)LDL (mg/dl)114.5 (78.5-140.8)TG (mg/dl)139.50 (89.25-191.20)Expressed in median and interquartiles.Disclosure of Interests:None declared

The pandemic of COVID-19 has afflicted every individual and has initiated a cascade of directly or indirectly involved events in precipitating mental health issues. The human species is a wanderer and hunter-gatherer by nature, and physical social distancing and nationwide lockdown have confined an individual to physical isolation. The present review article was conceived to address psychosocial and other issues and their aetiology related to the current pandemic of COVID-19. The elderly age group has most suffered the wrath of SARS-CoV-2, and social isolation as a preventive measure may further induce mental health issues. Animal model studies have demonstrated an inappropriate interacting endogenous neurotransmitter milieu of dopamine, serotonin, glutamate, and opioids, induced by social isolation that could probably lead to observable phenomena of deviant psychosocial behavior. Conflicting and manipulated information related to COVID-19 on social media has also been recognized as a global threat. Psychological stress during the current pandemic in frontline health care workers, migrant workers, children, and adolescents is also a serious concern. Mental health issues in the current situation could also be induced by being quarantined, uncertainty in business, jobs, economy, hampered academic activities, increased screen time on social media, and domestic violence incidences. The gravity of mental health issues associated with the pandemic of COVID-19 should be identified at the earliest. Mental health organization dedicated to current and future pandemics should be established along with Government policies addressing psychological issues to prevent and treat mental health issues need to be developed. References World Health Organization (WHO) Coronavirus Disease (COVID-19) Dashboard. 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Teacher's abilities to understand the benefits and use of media literacy play an important role in dealing with children as digital natives. Media literacy education can be an instrument through the use of blended-learning websites to address the challenges of education in the 21st century and learning solutions during and after the Covid-19 pandemic. This study aims to figure the teacher's perspective in understanding media literacy as an instrument for implementing blended-learning in early-childhood classes. Using a qualitative approach, this study combines two types of data. Data collection involved kindergarten teachers, six people as informants who attended the interviews and twenty-six participants who filled out questionnaires. Typological data analysis was used for qualitative data as well as simple statistical analysis to calculate the percentage of teacher perspectives on questionnaires collected the pandemic. The findings show five categories from the teacher's perspective. First, about the ability to carry out website-based blended-learning and the use of technology in classrooms and distance learning is still low. It must be transformed into more creative and innovative one. Encouraging teacher awareness of the importance of media literacy education for teachers as a more effective integrated learning approach, especially in rural or remote areas, to be the second finding. Third, national action is needed to change from traditional to blended-learning culture. Fourth, the high need for strong environmental support, such as related-party policies and competency training is the most important finding in this study. Finally, the need for an increase in the ease of access to technology use from all related parties, because the biggest impact of the Covid-19 pandemic is on ECE, which is closely related to the perspective of teachers on technology. The research implication demands increase in technology systems and connections between educators, parents, institutional managers, and education policy holders, for ECE services in urban areas for disadvantaged children, and all children in rural or remote areas. Keywords: Blended Learning, Early Childhood Classroom, Media Literacy Education References Aktay, S. (2009). The ISTE national educational technology standards and prospective primary school teachers in Turkey. International Journal of Learning, 16(9), 127–138. https://doi.org/10.18848/1447-9494/cgp/v16i09/46607 Arke, E. T., & Primack, B. A. (2009). Quantifying media literacy: Development, reliability, and validity of a new measure. Educational Media International, 46(1), 53–65. https://doi.org/10.1080/09523980902780958 Briquet-Duhazé, S. (2019). Websites Consulted by Future Primary Level Schoolteachers in France: Differences between Students and Trainees. 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BackgroundMultiple genomics-based biomarkers of response to immune checkpoint inhibition have been reported or proposed, including tumor mutation/neoantigen frequency, PD-L1 expression, T cell receptor repertoire clonality, interferon gene signature expression, HLA expression, and others.1 Although genomics associations of response have been reported, the primary studies have used a variety of data generation and processing techniques. There is a need for data harmonization and assessment of generalizability of potential biomarkers across multiple datasets.MethodsWe acquired patient-level RNA sequencing FASTQ data files from 10 data sets reported in seven pan-cancer PD-1 and CTLA-4 immune checkpoint inhibition trials with matched clinical annotations.2–7 We applied a common bioinformatics workflow for quality control, mapping to reference (STAR), generating gene expression matrices (SALMON), T cell receptor repertoire inference (MiXCR), extraction of immune gene signatures and immune subtypes,8 and differential gene expression analysis (DESeq2). We analyzed i) immunogenomics features proposed as biomarkers, and ii) gene expression signatures built from each trial for association with overall survival across the set of trials using univariable Cox proportional hazards regression. In all, we assessed 9 total immunogenomics features/signatures. P-values were adjusted for multiple testing using the Benjamini-Hochberg method.ResultsOf the 9 immunogenomics features assessed, cytolytic activity score and expression of the Follicular Dendritic Cell Secreted Protein gene (FDCSP) were associated with survival in two of seven studies, respectively (adjusted p < 0.05) (figure 1). No proposed biomarkers were significantly associated with survival in more than two studies. The sets of genes significantly associated with clinical benefit across the studies were highly disjoint, with only three genes significant in three studies and thirteen genes significant in two studies (figure 2). No genes were significantly associated with clinical benefit in more than three of seven studies.Abstract 75 Figure 1Association of immunogenomics features and proposed biomarkers with survival in 10 publicly available datasets from 7 clinical trials with immune checkpoint blockade. Nine immunogenomics features were tested in 10 publicly available RNAseq data sets from 7 published clinical trials with immune checkpoint blockade for their correlation with outcome. SKCM, skin cutaneous melanoma; BLCA, bladder cancer; Kidney, kidney cancer; Ureter, ureteral cancer; GBM, glioblastomaAbstract 75 Figure 2Association of gene expression of single genes with survival in 10 publicly available datasets from 7 clinical trials with immune checkpoint inhibitorsConclusionsNo proposed biomarkers were highly generalizable across studies. We expect that integrated modeling incorporating multiple immunogenomics features will be required to build a robust and generalizable biomarker for ICI response. Further work is needed to analyze determinants of response and clinical benefit.AcknowledgementsWe would like to thank SITC for funding for this work as part of the Sparkathon TimIOS collaborative project.ReferencesZappasodi R, Wolchok JD, Merghoub T. Strategies for Predicting Response to Checkpoint Inhibitors. Curr Hematol Malig Rep 2018;13(5):383–95.Liu D, Schilling B, Liu D, Sucker A, Livingstone E, Jerby-Arnon L, Zimmer L, Gutzmer R, Satzger I, Loquai C, Grabbe S, Vokes N, Margolis CA, Conway J, He MX, Elmarakeby H, Dietlein F, Miao D, Tracy A, Gogas H, Goldinger SM, Utikal J, Blank CU, Rauschenberg R, von Bubnoff D, Krackhardt A, Weide B, Haferkamp S, Kiecker F, Izar B, Garraway L, Regev A, Flaherty K, Paschen A, Van Allen EM, Schadendorf D. Integrative molecular and clinical modeling of clinical outcomes to PD1 blockade in patients with metastatic melanoma. 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Climate change induced sea-level rise (SLR) is on its increase globally. Regionally the lowlands of China, Vietnam, Bangladesh, and islands of the Malaysian, Indonesian and Philippine archipelagos are among the world’s most threatened regions. Sea-level rise has major impacts on the ecosystems and society. It threatens coastal populations, economic activities, and fragile ecosystems as mangroves, coastal salt-marches and wetlands. This paper provides a summary of the current state of knowledge of sea level-rise and its effects on both human and natural ecosystems. The focus is on coastal urban areas and low lying deltas in South-East Asia and Vietnam, as one of the most threatened areas in the world. About 3 mm per year reflects the growing consensus on the average SLR worldwide. The trend speeds up during recent decades. The figures are subject to local, temporal and methodological variation. In Vietnam the average values of 3.3 mm per year during the 1993-2014 period are above the worldwide average. Although a basic conceptual understanding exists that the increasing global frequency of the strongest tropical cyclones is related with the increasing temperature and SLR, this relationship is insufficiently understood. Moreover the precise, complex environmental, economic, social, and health impacts are currently unclear. SLR, storms and changing precipitation patterns increase flood risks, in particular in urban areas. Part of the current scientific debate is on how urban agglomeration can be made more resilient to flood risks. Where originally mainly technical interventions dominated this discussion, it becomes increasingly clear that proactive special planning, flood defense, flood risk mitigation, flood preparation, and flood recovery are important, but costly instruments. Next to the main focus on SLR and its effects on resilience, the paper reviews main SLR associated impacts: Floods and inundation, salinization, shoreline change, and effects on mangroves and wetlands. The hazards of SLR related floods increase fastest in urban areas. This is related with both the increasing surface major cities are expected to occupy during the decades to come and the increasing coastal population. In particular Asia and its megacities in the southern part of the continent are increasingly at risk. The discussion points to complexity, inter-disciplinarity, and the related uncertainty, as core characteristics. An integrated combination of mitigation, adaptation and resilience measures is currently considered as the most indicated way to resist SLR today and in the near future.References Aerts J.C.J.H., Hassan A., Savenije H.H.G., Khan M.F., 2000. Using GIS tools and rapid assessment techniques for determining salt intrusion: Stream a river basin management instrument. 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This study analyzed the antibacterial activity of curry leaf extract (Murraya koenigii) on the growth of Edwardsiella tarda bacteria. This study aims to determine the bioactivity and antibacterial effectiveness of M. koenigii leaf extract against the growth of E.tarda bacteria. Inhibition test was carried out by delusion (MIC test) and diffusion (disc test) methods. MIC test used 5 variations of concentration: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L and 1,000 mg/L on TSB (Tryptone Soya Broth) media; it was incubated for 24 hours. While the disc test used 5 variations of concentration: 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L and 500 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. Chloramphenicol (5 mg/L) was used as a positive control, and distilled water was used as a negative control. M. koenigii leaf extract contains natural bioactive; it was bacteriostatic antibacterial due to bacteria's growth after 48 hours incubation. The highest inhibition diameter of E.tarda was 7,20 mm at a concentration of 500 mg/L after 24 hours incubation. The highest inhibitory effectiveness was at a concentration of 500 mg/L with effectivity 56.3%, while it declined to 46,44% after 48 hours incubation.

Abstract Introduction: BCR-ABL kinase domain (KD) mutations have well established role in tyrosine kinase inhibitors (TKIs) resistance and disease progression in chronic myeloid leukemia (CML)1. In recent years, compound BCR-ABL mutations have emerged as a new threat to CML patients by causing higher degrees of resistance involving multiple TKIs, including ponatinib2. However, there are limited reports about association of compound BCR-ABL mutations with disease progression in imatinib sensitive CML patients3. Furthermore, BCR-ABL mutation detection is currently recommended only in case of drug resistance and disease progression and clinical significance of BCR-ABL mutation detection in TKI responder chronic phase CML is not well documented4. Therefore, we investigated presence of ABL-KD mutations in chronic phase (CP) and advanced phase imatinib sensitive CML to find out association of BCR-ABL mutations with progression to advanced disease phases in CML patients. . Patients and Methods: Imatinib sensitive CML patients in CP and advanced phases of the disease were included in the study.All CP patients were incomplete hematological, complete cytogenetic and major molecular responses. Due to specific study objectives, patients manifesting drug resistance during follow-up studies were excluded from the study. A total of 90 imatinib sensitive CML patients (CP=41, late CP=33, and accelerated phase=16) were finally available for analysis. All patients as well as 10 healthy controls were investigated for BCR-ABL mutations using Sanger sequencing. All response criteria were per European LeukemiaNet guidelines4. Data was analyzed using SPSS software (version 19). Results: Mean age of the patients was 33 years (Table 1). Eleven out of 33 (33.3%) patients in late-CP CML harbored 24 types of point mutations, out of which eight (72.72%) harbored compound mutated sites (Figure 1, Table 2). E355G (3.33%) was the most prevalent mutant. Five patients (45.45%), all of which had compound mutations, progressed to advanced phases of disease during follow up studies. No BCR-ABL mutation was detected in healthy subjects and in early CP CML patients. Therefore, early CP CML patients served as additional control in this study. BCR-ABL mutations were found in 3 accelerated phase patients as well. Late-CP mutations were associated with elevated platelet count (p= 0.037) and male gender (p= 0.049). The median overall survival and event free survival of CML patients (n=90) was 6.98 and 5.8 years respectively. Seven year survival was found to be 94.2%. Discussion and conclusions: Compound BCR-ABL mutations were associated with progression to advanced disease in imatinib sensitive late-CP CML patients. Although single BCR-ABL mutations have previously been found to cause CML progression5, this is first report of association of compound BCR-ABL mutations with disease progression in stable imatinib responders at late CP. Detection of new mutations can help in defining mechanism of CML progression6. Moreover, BCR-ABL mutation detection in late CP CML patients sensitive to TKI treatment can help in early assessment of risk for disease progression and/or drug resistance and subsequent clinical intervention to delay disease progression which is a major challenge of CML therapy in TKI era7. References: 1. Haznedaroglu IC. Mediterranean journal of hematology and infectious diseases 2015;7. 2. Khorashad JS, Kelley TW, Szankasi P, Mason CC, Soverini S, Adrian LT, Eide CA, Zabriskie MS, Lange T, Estrada JC. Blood 2013;121:489-98. 3. Deininger MW, Hodgson JG, Shah NP, Cortes JE, Kim DW, Nicolini FE, Talpaz M,Baccarani M, Müller MC, Li J, Parker WT, Lustgarten S, Clackson T, Haluska FG,Guilhot F, Kantarjian HM, Soverini S, Hochhaus A, Hughes TP, Rivera VM, Branford S. Blood. 2016 Feb 11;127(6):703-12.dddd 4. 7. Baccarani M, Deininger MW, Rosti G, Hochhaus A, Soverini S, Apperley JF, Cervantes F, Clark RE, Cortes JE, Guilhot F. Blood 2013;122:872-84. 5. Carella AM, Garuti A, Cirmena G, Catania G, Rocco I, Palermo C, Pica G, Pierri I, Miglino M, Ballestrero A, Gobbi M, Patrone F. Leukemia & lymphoma 2010;51:275-8. 6. Giotopoulos G, van der Weyden L, Osaki H, Rust AG, Gallipoli P, Meduri E,Horton SJ, Chan WI, Foster D, Prinjha RK, Pimanda JE, Tenen DG, Vassiliou GS,Koschmieder S, Adams DJ, Huntly BJ. J Exp Med. 2015 Sep 21;212(10):1551-69. 7. Mukherjee S, Kalaycio M. Curr Hematol Malig Rep. 2016 Apr;11(2):86-93. Disclosures No relevant conflicts of interest to declare.

BackgroundThe landscape of cancer treatment has changed drastically since the development of Immune Checkpoint Inhibitors (ICI). ICIs have become the cornerstone to various cancer treatments.1 2 The adverse effect (AE) profile of ICI is different than conventional chemotherapy. Nivolumab and pembrolizumab target programmed cell death-1 T-cell receptor, whereas programmed death ligand-1 is targeted by atezolizumab and durvalumab.3 4 Easy tolerability and lack of myelosuppresion make immunotherapy an attractive treatment option. Some AEs can be severe, life-threatening, or even fatal (grade III and higher).1 Much of the data regarding AE profile is from clinical trials. The aim of our study is to review real world single institution AE data on the most commonly utilized ICIs.MethodsWe reviewed a total of 229 patient charts who had received pembrolizumab, nivolumab, durvalumab or atezolizumab at Saint Francis Hospital in Hartford, CT, USA. 53 patients were excluded given lack of records or because they received less than 2 cycles of treatment.Results176 patients were included in the final analysis. ICIs were discontinued in 25/176 (14.2%) patients secondary to AE. 24/176 (13.6%) patients had grade III or higher AEs reported. 10/95 (10.5%) patients who received pembrolizumab developed grade III/IV AEs (8 pneumonitis, 2 nephritis). 5/45 (11.1%) patients treated with nivolumab developed grade III/IV AEs (2 pneumonitis, 1 new-onset DKA, 1 nephritis, 1 myositis). 8/19 (42.1%) receiving durvalumab had grade III or higher AEs (6 pneumonitis, 1 sepsis, 1 colitis). Lastly, 1/17 (5.8%) in atezolizumab group developed grade III/IV AE (colitis). 96/176 (54.5%) patients had one or more ER visit and 91/176 (51.7%) were admitted to the hospital for various reasons one or more times.ConclusionsICIs have a relatively safe drug profile. 86.4% of our studied population did not develop any grade III or higher AEs. The main reason for ICI discontinuation was disease progression rather than AE. The most common grade III/IV AE was pneumonitis. Durvalumab had the highest incidence of AE, pneumonitis, which is likely related to radiation use prior to immunotherapy.AcknowledgementsN/AEthics ApprovalInstitutional Review Board approval - IRB#: SFH-19–72ReferencesAzoury SC, Straughan DM, Shukla V. Immune checkpoint inhibitors for cancer therapy: clinical efficacy and safety. Curr Cancer Drug Targets 2015;15(6):452–62.Kyi C, Postow MA. Checkpoint blocking antibodies in cancer immunotherapy. FEBS Lett. 2014 Jan 21;588(2):368–76. doi: 10.1016/j.febslet.2013.10.015.Kroschinsky F, Stölzel F, von Bonin S, Beutel G, Kochanek M, Kiehl M, Schellongowski P; Intensive Care in Hematological and Oncological Patients (iCHOP) Collaborative Group. New drugs, new toxicities: severe side effects of modern targeted and immunotherapy of cancer and their management. Crit Care 2017 Apr 14;21(1):89. doi: 10.1186/s13054-017-1678-1.Hofmann L, Forschner A, Loquai C, Goldinger SM, Zimmer L, Ugurel S, Schmidgen MI, Gutzmer R, Utikal JS, Heinzerling LM, et al. Cutaneous, gastrointestinal, hepatic, endocrine, and renal side-effects of anti-PD-1 therapy. Eur J Cancer 2016 Jun;60:190–209. doi: 10.1016/j.ejca.2016.02.025.

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Murraya koenigii L. (family: Rutaceae) commonly called as curry leaf is a highly valued plant for aroma and medicinal value. The two dominant AM species Glomus mosseae and Acaulospora laevis were isolated from the rhizospheric soil of M. koenigii. A pot experiment was performed to see the interactive potential of G. mosseae and A. laevis alone or in combination with Pseudomonas fluorescens on M. koenigii. Various morphological and biochemical parameters were measured after 120 days. Overall results suggest that although, all co-inoculation treatments showed beneficial effects on all the growth, physiological and oil content. The overall results demonstrate that the co-inoculation of bioinoculants like P. fluorescens with AM fungi promotes higher AM colonization and spore number enhancing nutrient acquisition especially phosphorus (P), improving the rhizospheric condition of soil.

We sequenced two alleles of the MATa locus of Saccharomyces cerevisiae that reduce homothallic switching and confer viability to HO rad52 strains. Both the MATa-stk (J. E. Haber, W. T. Savage, S. M. Raposa, B. Weiffenbach, and L. B. Rowe, Proc. Natl. Acad. Sci. USA 77:2824-2828, 1980) and MATa-survivor (R. E. Malone and D. Hyman, Curr. Genet. 7:439-447, 1983) alleles result from a T----A base change at position Z11 of the MAT locus. These strains also contain identical base substitutions at HMRa, so that the mutation is reintroduced when MAT alpha switches to MATa. Mating-type switching in a MATa-stk strain relative to a MATa Z11T strain is reduced at least 50-fold but can be increased by expression of HO from a galactose-inducible promoter. We confirmed by Southern analysis that the Z11A mutation reduced the efficiency of double-strand break formation compared with the Z11T variant; the reduction was more severe in MAT alpha than in MATa. In MAT alpha, the Z11A mutation also creates a mat alpha 1 (sterile) mutation that distinguishes switches of MATa-stk to either MAT alpha or mat alpha 1-stk. Pedigree analysis of cells induced to switch in G1 showed that MATa-stk switched frequently (23% of the time) to produce one mat alpha 1-stk and one MAT alpha progeny. This postswitching segregation suggests that Z11 was often present in heteroduplex DNA that was not mismatch repaired. When mismatch repair was prevented by deletion of the PMS1 gene, there was an increase in the proportion of mat alpha 1-stk/MAT alpha sectors (59%) and in pairs of switched cells that both retained the stk mutation (27%). We conclude that at least one strand of DNA only 4 bp from the HO cut site is not degraded in most of the gene conversion events that accompany MAT switching.

We sequenced two alleles of the MATa locus of Saccharomyces cerevisiae that reduce homothallic switching and confer viability to HO rad52 strains. Both the MATa-stk (J. E. Haber, W. T. Savage, S. M. Raposa, B. Weiffenbach, and L. B. Rowe, Proc. Natl. Acad. Sci. USA 77:2824-2828, 1980) and MATa-survivor (R. E. Malone and D. Hyman, Curr. Genet. 7:439-447, 1983) alleles result from a T----A base change at position Z11 of the MAT locus. These strains also contain identical base substitutions at HMRa, so that the mutation is reintroduced when MAT alpha switches to MATa. Mating-type switching in a MATa-stk strain relative to a MATa Z11T strain is reduced at least 50-fold but can be increased by expression of HO from a galactose-inducible promoter. We confirmed by Southern analysis that the Z11A mutation reduced the efficiency of double-strand break formation compared with the Z11T variant; the reduction was more severe in MAT alpha than in MATa. In MAT alpha, the Z11A mutation also creates a mat alpha 1 (sterile) mutation that distinguishes switches of MATa-stk to either MAT alpha or mat alpha 1-stk. Pedigree analysis of cells induced to switch in G1 showed that MATa-stk switched frequently (23% of the time) to produce one mat alpha 1-stk and one MAT alpha progeny. This postswitching segregation suggests that Z11 was often present in heteroduplex DNA that was not mismatch repaired. When mismatch repair was prevented by deletion of the PMS1 gene, there was an increase in the proportion of mat alpha 1-stk/MAT alpha sectors (59%) and in pairs of switched cells that both retained the stk mutation (27%). We conclude that at least one strand of DNA only 4 bp from the HO cut site is not degraded in most of the gene conversion events that accompany MAT switching.

A blight (wet rot) of petunia (Petunia ×hybrida Hort. Vilm.-Andr.) was observed in a wholesale propagation nursery in Baton Rouge, LA in September 2002. The grower reported that plants wilted and then completely rotted. The disease occurred during a period of hot, humid, and cloudy weather. Approximately 100 flats of flowering-age plants of cvs. Rose and White Madness were destroyed. No fungal sporulation was noticed on dead plants, but occasional strands of white mycelium were observed. The grower's use of azoxystrobin, iprodione, and thiophanate methyl plus mancozeb fungicides during current and past outbreaks of this disease did not prevent disease spread, but disease activity stopped after temperature and humidity dropped in early October. A fungus that produced white aerial mycelia that later developed light yellow areas and also black aerial spore masses was consistently isolated from diseased tissue placed on acidified potato dextrose agar (APDA). The fungus was identified as Choanephora cucurbitarum (Berk. & Ravenel) Thaxt. on the basis of cultural and morphological characteristics (3). Sporangiola were ellipsoid, pale brown to reddish brown with distinct longitudinal striations and measured 15 to 20 × 9 to 14 μm. Sporangiospores were broadly ellipsoid, pale brown to reddish brown, indistinctly striate with fine, hyaline polar appendages, and measured 16 to 34 × 7 to 12 μm. Spore measurements were within the range previously given for C.cucurbitarum (3). Pathogenicity tests were performed by misting a mixture of sporangiola and sporangiospores (25,000 to 70,000 per ml of water taken from 7- to 10-day-old cultures grown on APDA) on flowering-age petunia plants (cvs. Rose Madness, White Madness, and Dreams Pink). Tests were repeated twice. Inoculated plants and uninoculated control plants (2 to 4 of each treatment in each test) were held in a dew chamber at 28°C for 48 h and then moved to a greenhouse. Within 48 h after inoculation, plants developed water-soaked lesions on flowers, leaves, and stems, then wilted and rotted. Uninoculated plants remained disease free except for several that developed disease symptoms in the first test, apparently from the presence of natural inoculum on healthy-appearing plants that were obtained from the nursery where the disease was found. Koch's postulates were completed by reisolation of the pathogen from diseased inoculated plants. C. cucurbitarum (1) and C. infundibulifera (Curr.) Sacc. (2) have been reported to cause flower blight of petunia in the United States and whole plant blight (wet rot) of petunia in Japan (4). To our knowledge, this is the first report of C. cucurbitarum causing whole plant blight of petunia in the United States. References: (1) M. L. Daughtrey et al. Choanephora wet rot of poinsettia. Page 15 in: Compendium of Flowering Potted Plant Diseases. The American Phytopathological Society, St. Paul, MN, 1995. (2) D. F. Farr et al. Fungi on Plants and Plant Products in the United States. The American Phytopathological Society, St. Paul, MN, 1989. (3) P. M. Kirk. Mycological Paper 152:1, 1984. (4) J. Takeuchi and H. Horie. Jpn. J. Phytopathol. 66:72, 2000.

-Valerie I.J. Flint, Margarita Zamora, Reading Columbus. Berkeley: University of California Press, 1993. xvi + 247 pp.-Riva Berleant-Schiller, Historie Naturelle des Indes: The Drake manuscript in the Pierpont Morgan Library. New York: Norton, 1996. xxii + 272 pp.-Neil L. Whitehead, Charles Nicholl, The creature in the map: A journey to Eldorado. London: Jonathan Cape, 1995. 398 pp.-William F. Keegan, Ramón Dacal Moure ,Art and archaeology of pre-Columbian Cuba. Pittsburgh: University of Pittsburgh Press, 1996. xxiv + 134 pp., Manuel Rivero de la Calle (eds)-Michael Mullin, Stephan Palmié, Slave cultures and the cultures of slavery. Knoxville: University of Tennessee Press, 1995. xlvii + 283 pp.-Bill Maurer, Karen Fog Olwig, Small islands, large questions: Society, culture and resistance in the post-emancipation Caribbean. London: Frank Cass, 1995. viii + 200 pp.-David M. Stark, Laird W. Bergad ,The Cuban slave market, 1790-1880. Cambridge: Cambridge University Press, 1995. xxi + 245 pp., Fe Iglesias García, María Del Carmen Barcia (eds)-Susan Fernández, Tom Chaffin, Fatal glory: Narciso López and the first clandestine U.S. war against Cuba. Charlottesville: University Press of Virginia, 1996. xxii + 282 pp.-Damian J. Fernández, María Cristina García, Havana USA: Cuban exiles and Cuban Americans in South Florida, 1959-1994. Berkeley: University of California Press, 1996. xiii + 290 pp.-Myrna García-Calderón, Carmen Luisa Justiniano, Con valor y a cómo dé lugar: Memorias de una jíbara puertorriqueña. Río Piedras: Editorial de la Universidad de Puerto Rico, 1994. 538 pp.-Jorge Pérez-Rolon, Ruth Glasser, My music is my flag: Puerto Rican musicians and their New York communities , 1917-1940. Los Angeles: University of California Press, 1995. xxiv + 253 pp.-Lauren Derby, Emelio Betances, State and society in the Dominican Republic. Boulder CO: Westview Press, 1995. xix + 162 pp.-Michiel Baud, Bernardo Vega, Trujillo y Haiti, Volumen II (1937-1938). Santo Domingo: Fundación Cultural Dominicana, 1995. 427 pp.-Danielle Bégot, Elborg Forster ,Sugar and slavery, family and race: The letters and diary of Pierre Dessalles, Planter in Martinique, 1808-1856. Elborg & Robert Forster (eds. and trans.). Baltimore: John Hopkins University Press, 1996. 322 pp., Robert Forster (eds)-Catherine Benoit, Richard D.E. Burton, La famille coloniale: La Martinique et la mère patrie, 1789-1992. Paris: L'Harmattan, 1994. 308 pp.-Roderick A. McDonald, Kathleen Mary Butler, The economics of emancipation: Jamaica & Barbados, 1823-1843. Chapel Hill: University of North Carolina Press, 1995. xviii + 198 pp.-K.O. Laurence, David Chanderbali, A portrait of Paternalism: Governor Henry Light of British Guiana, 1838-48. Turkeyen, Guyana: Dr. David Chanderbali, Department of History, University of Guyana, 1994. xiii + 277 pp.-Mindie Lazarus-Black, Brian L. Moore, Cultural power, resistance and pluralism: Colonial Guyana 1838-1900. Montreal & Kingston: McGill-Queen's University Press; Mona, Kingston: The Press-University of the West Indies, 1995. xv + 376 pp.-Madhavi Kale, K.O. Laurence, A question of labour: Indentured immigration into Trinidad and British Guiana, 1875-1917. Kingston: Ian Randle; London: James Currey, 1994. ix + 648 pp.-Franklin W. Knight, O. Nigel Bolland, On the March: Labour rebellions in the British Caribbean, 1934-39. Kingston: Ian Randle; London: James Currey, 1995. viii + 216 pp.-Linden Lewis, Kevin A. Yelvington, Producing power: Ethnicity, gender, and class in a Caribbean workplace. Philadelphia: Temple University Press, 1995. xv + 286 pp.-Consuelo López Springfield, Alta-Gracia Ortíz, Puerto Rican women and work: Bridges in transnational labor. Philadelphia: Temple University Press, 1996. xi + 249 pp.-Peta Henderson, Irma McClaurin, Women of Belize: Gender and change in Central America. New Brunswick NJ: Rutgers University Press, 1996. x + 218 pp.-Bonham C. Richardson, David M. Bush ,Living with the Puerto Rico Shore. José Gonzalez Liboy & William J. Neal. Durham: Duke University Press, 1995. xx + 193 pp., Richard M.T. Webb, Lisbeth Hyman (eds)-Bonham C. Richardson, David Barker ,Environment and development in the Caribbean: Geographical perspectives. Mona, Kingston: The Press-University of the West Indies, 1995. xv + 304 pp., Duncan F.M. McGregor (eds)-Alma H. Young, Anthony T. Bryan ,Distant cousins: The Caribbean-Latin American relationship. Miami: North-South-Center Press, 1996. iii + 132 pp., Andrés Serbin (eds)-Alma H. Young, Ian Boxill, Ideology and Caribbean integration. Mona, Kingston: The Press-University of the West Indies, 1993. xiii + 128 pp.-Stephen D. Glazier, Howard Gregory, Caribbean theology: Preparing for the challenges ahead. Mona, Kingston: Canoe Press, University of the West Indies, 1995. xx + 118 pp.-Lise Winer, Richard Allsopp, Dictionary of Caribbean English usage. With a French and Spanish supplement edited by Jeanette Allsopp. Oxford: Oxford University Press, 1996. lxxviii + 697 pp.-Geneviève Escure, Jacques Arends ,Pidgins and Creoles: An introduction. Amsterdam/Philadelphia: John Benjamins, 1995. xiv + 412 pp., Pieter Muysken, Norval Smith (eds)-Jacques Arends, Angela Bartens, Die iberoromanisch-basierten Kreolsprachen: Ansätze der linguistischen Beschreibung. Frankfurt am Main: Peter Lang, 1995. vii + 345 pp.-J. Michael Dash, Richard D.E. Burton, Le roman marron: Études sur la littérature martiniquaise contemporaine. Paris: L'Harmattan. 1997. 282 pp.

Root and basal stem rot, blighting, and wilting have been observed on Epipremnum aureum (Linden ex André) plants in many nurseries in and near Buenos Aires since 1997. Infected stem tissues show an intense dark brown discoloration and water soaking near the stem base that eventually leads to plant death. To determine the causal agent of the disease, small pieces of diseased tissue were surface-sterilized for 2 min in 2% sodium hypochlorite and plated on potato-dextrose agar (PDA). Whitish colonies that eventually turned brown developed in 2 to 3 days at 22 to 24°C. Irregularly shaped sclerotia were observed. Isolates typical of Rhizoctonia solani Kuhn exhibited mycelia with branches inclined in the direction of growth, constricted at the point of union with the main hyphae, with a septum in the branch near the constriction. No telemorph was observed. Nuclei in living hyphal mats were stained directly on a microscope slide coated with water agar according to the method of Tu and Kimbrough (4) and were examined at 400× magnification. The cells were multinucleate. Anastomosis group was determined by using known tester isolates of Rhizoctonia spp. (3). Positive anastomosis was observed with tester strains of AG-4 HG-II. The polymerase chain reaction was performed according to the protocol of Boysen et al (1) in order to confirm the anastomosis group. Primers used for the amplification of the ITS region were ITSI and LROR. Amplification of the ITS region indicated lack of variation with AG-4 tester strain. The pathogenicity of the isolate was determined with the inoculum-layer technique (2), consisting of a 7-day-old petri plate culture of the pathogen in PDA that is removed from the dish and placed intact on the soil, 2 to 4 cm under the roots of 10 healthy plants. Some leaves of the plants were placed in contact with the inoculated substratum. For a control, PDA was placed under the roots of other plants. Plants were maintained at 22 to 24°C, with close-to-saturation humidity. After 6 to 10 days, symptoms were similar to those previously observed. Initially leaves that had been placed in contact with the substratum showed dark areas with a watersoaked area 2 to 3 cm in diameter. These lesions expanded over the entire leaf blade moving into the petioles and stems killing the plant. One hundred percent of inoculated plants were infected. Koch's postulates were satisfied after reisolating the fungus. The characteristics of the causal agent are those of multinucleate isolates of R. solani belonging to the anastomosis group AG-4 HG-II (3). This is the first report of R. solani causing disease on E. aureum in Argentina. References: (1) M. Boysen, M. Borja, C. Del Corral, O. Salazar, and V. Rubio. Curr. Genet. 29:174–181, 1996. (2) A. F. Schmitthenner and J. W. Hilty. Phytopathology 52:177–178, 1962. (3) B. Sneh, L. Burpee, and A. Ogoshi. 1991. Identification of Rhizoctonia Species. The American Phytopathological Society, St. Paul, MN. (4) C. C. Tu and J. W. Kimbrough. Mycologia 65:941–944, 1973.

Chronological aging of the hematopoietic compartment is associated with decreased bone marrow cellularity, reduced lymphopoiesis, increased anemia, a myeloid proliferation bias and an increased incidence of myeloid cancers. Beerman et al. proposed that this age-related myeloid lineage favoritism may be explained by clonal expansion of intrinsically myeloid-biased hematopoietic stem cells with robust self-renewal potential(1). This age-associated clonal expansion was initially suspected by X-chromosome inactivation (XCI) studies performed in the normal aging population, which documented a skewed XCI pattern in a significant proportion of women over 60 year-old(2). More recently, genome wide approaches led several groups to document au augmented prevalence of acquired clonal copy number changes (3,4,5) or clonal somatic mutations with increasing age (6,7,8,9). The most frequently mutated genes are the same as those documented in myeloid cancers, such as TET2, DNMT3A, ASXL1, PPM1D, GNAS, TP53, JAK2 and SF3B1 among others. The prevalence of these age-associated mutations may reach > 10% of older individuals, and is associated with an 11-12 fold increased relative risk of developing hematological malignancies. However, the actual problematic is to define the prognostic significance of these clonal mutations in the aging population. Steensma et al. proposed to consider these mutations as «Clonal Hematopoiesis of Indeterminate Potential (CHIP)»(10). The goal of our research group is to define the oncogenic penetrance of CHIP by applying a precision medicine approach in a large prospective cohort (n=4000) of aging individuals comprised of related and unrelated subjects. The variables under investigation include, clonality by XCI in women, deep sequencing (NGS) of myeloid cancer associated genes, epigenetic markers (5hmC, 5mC), telomere length, blood counts, heritability and outcome. PRELIMINARY RESULTS. XCI analyses Acquired skewing of XCI predominantly affects the myeloid lineage with a prevalence of 41.4% for PMN and is age dependent (r=0.15, P<10-4), in contrast to T cells 22.5%. These results support the idea of an age-associated clonal myeloid expansion. NGS of myeloid gene panel. We documented a prevalence of 17.9% of mutated individuals. Mutations were mainly documented in TET2 and DNMT3A which accounted for 90% of all identified mutations. Other significantly mutated genes included JAK2, ASXL1, CBL, TP53 and KRAS. Double mutations were identified in 2.5% of individuals (14% of the mutated individuals) and half of them had concomitant mutation in TET2 and DNMT3A. Age and XCI skewing was similar between subjects with mutation in TET2 or DNMT3A, but slightly higher in double mutants. Epigenetic markers. Subjects with mutation in TET2 had a significant reduction in 5hmC level that correlated with Variable Allele Frequency (VAF) of the mutation. No specific global epigenetic phenotype was documented in the DNMT3A mutation subgroup. We also documented an age-associated reduction in 5hmC that was independent of acquired mutation in the TET2 gene. Taken together these results indicate that age-associated clonal mutations involves predominantly two genes (TET2 and DNMT3A), suggesting that alteration of epigenetic maintenance is a central to the initiation of clonal dominance. Completion of investigation of the aging cohort and prospective follow-up will help characterize the link between aging hematopoiesis and the development of myeloid cancers. 1. Beerman I, Maloney WJ, Weissmann IL, et al. Stem cells and the aging hematopoietic system. Curr Opin Immunol. 2010;22(4):500-506. 2. Busque L, Mio R, Mattioli J, et al. Non-random X-inactivation patterns in normal females: lyonization ratios vary with age. Blood. 1996;88(1):59-65. 3. Forsberg LA, Rasi C, Razzaghian HR, et al. Age-related somatic structural changes in the nuclear genome of human blood cells. AJHG, 2012;90:217-228. 3. Laurie CC, Laurie CA, Rice K, et al. Detectable clonal mosaicism from birth to old age and its relationship to cancer. Nat Genet. 2012;44(6):642-650. 4. Jacobs KB, Yeager M, Zhou W, et al. Detectable clonal mosaicism and its relationship to aging and cancer. Nat Genet. 2012;44(6):651-658. 5. Busque L, Patel JP, Figueroa ME, et al. Recurrent somatic TET2 mutation in normal elderly individuals with clonal hematopoiesis. Nat Genet. 2012;444(11):1179-1181. 6. Xie M, Lu C, Wang J, et al. Age-related mutations associated with clonal hematopoietic expansion and malignancies. Nat Med. 2014;20(12):1472-1478. 7. Genovese G, Kähler AK, Handsaker RE, et al. Clonal hematopoiesis and blood-cancer risk inferred from blood DNA sequence. N Engl J Med. 2014;371(26):2477-2487. 8. Jaiswal S, Fontanillas P, Flannick J, et al. Age-related clonal hematopoiesis associated with adverse outcomes. N Engl J Med. 2014;371(26):2488-2498. 9.Steensma DP, Bejar R, Jaiswal S, et al. Clonal hematopoiesis of indeterminate potential and its distinction from myelodysplastic syndromes. Blood. 2015;126(1):9-16 Disclosures Busque: Pfizer: Consultancy, Honoraria; BMS: Consultancy, Honoraria; Novartis: Consultancy, Honoraria, Research Funding, Speakers Bureau.