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Journal articles on the topic 'CYP27A1'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Sawada, Natsumi, Toshiyuki Sakaki, Sachiko Kitanaka, Shigeaki Kato, and Kuniyo Inouye. "Structure-function analysis of CYP27B1 and CYP27A1." European Journal of Biochemistry 268, no. 24 (2001): 6607–15. http://dx.doi.org/10.1046/j.0014-2956.2001.02615.x.

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Nemazannikova, Natalie, Gregory L. Blatch, Crispin R. Dass, Rodney Sinclair, and Vasso Apostolopoulos. "Vitamin D enzymes (CYP27A1, CYP27B1, and CYP24A1) and receptor expression in non-melanoma skin cancer." Acta Biochimica et Biophysica Sinica 51, no. 4 (2019): 444–47. http://dx.doi.org/10.1093/abbs/gmy170.

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Abouzid, Mohamed, Łukasz Kruszyna, Dominika Kaczmarek, et al. "Genetic Polymorphism of CYP2R1, CYP27A1, CYP27B1, and Vitamin D Metabolites Plasma Levels in Patients with Cardiovascular Disease: A Pilot Study." Biomolecules 15, no. 5 (2025): 699. https://doi.org/10.3390/biom15050699.

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The active form of vitamin D, calcitriol (1,25(OH)2D3), is produced from 25(OH)D3 via enzymes encoded by CYP2R1, CYP27A1, and CYP27B1. Polymorphisms in these genes may alter vitamin D metabolism and increase cardiovascular disease risk. This preliminary study investigated these polymorphisms in 27 patients with cardiovascular disease and 26 healthy volunteers using Polymerase Chain Reaction—Restriction Fragment Length Polymorphism (PCR-RFLP), while measuring 25(OH)D3 and 1,25(OH)2D3 concentrations by UPLC-MS/MS and ELISA, respectively. Among patients, those with the GT genotype of rs10877012 (

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Mazanova, Anna, Ihor Shymanskyi, Olha Lisakovska, Lala Hajiyeva, Yulia Komisarenko, and Mykola Veliky. "Effects of Cholecalciferol on Key Components of Vitamin D-Endo/Para/Autocrine System in Experimental Type 1 Diabetes." International Journal of Endocrinology 2018 (2018): 1–9. http://dx.doi.org/10.1155/2018/2494016.

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Objectives.Recent prospective studies have found the associations between type 1 diabetes (T1D) and vitamin D deficiency. We investigated the role of vitamin D in the regulation of 25OHD-1α-hydroxylase (CYP27B1) and VDR expression in different tissues of T1D rats.Design.T1D was induced in male Wistar rats by streptozotocin (55 mg/k b.w.). After 2 weeks of T1D, the animals were treated orally with or without vitamin D3(cholecalciferol; 100 IU/rat, 30 days).Methods.Serum 25-hydroxyvitamin D (25OHD) was detected by ELISA. CYP27A1, CYP2R1, CYP27B1, and VDR were assayed by RT-qPCR and Western blott

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Hasan, Maruf, Michael Oster, Henry Reyer, et al. "Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs." Metabolites 12, no. 8 (2022): 729. http://dx.doi.org/10.3390/metabo12080729.

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Calcium (Ca) and phosphorus (P) homeostasis is maintained by several regulators, including vitamin D and fibroblast growth factor 23 (FGF23), and their tissue-specific activation and signaling cascades. In this study, the tissue-wide expression of key genes linked to vitamin D metabolism (CYP2R1, CYP27A1, CYP27B1, CYP24A1, GC, VDR) and FGF23 signaling (FGF23, FGFR1-4, KL) were investigated in pigs fed conventional (trial 1) and divergent P diets (trial 2). The tissue set comprised kidney, liver, bone, lung, aorta, and gastrointestinal tract sections. Expression patterns revealed that non-renal

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6

Cheng, Kai-Hung, Edward Hsi, Chia-Chu Liu, et al. "The Associations of Novel Vitamin D3Metabolic GeneCYP27A1Polymorphism, Adiponectin/Leptin Ratio, and Metabolic Syndrome in Middle-Aged Taiwanese Males." International Journal of Endocrinology 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/658151.

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Metabolic syndrome (MetS) confers increased risks of cardiovascular disease (CVD). Both vitamin D3and adipocytokines (especially adiponectin and leptin) have a great impact on CVD and MetS. In vitamin D3metabolism, the vitamin D325-hydroxylase (CYP27A1) and 25-hydroxyvitamin D31-alpha-hydroxylase (CYP27B1) are two key enzymes. This study aimed to examine the influence of vitamin D3CYP27 single nucleotide polymorphisms (SNPs) on adipocytokines and MetS. Cross-sectional data and DNA samples were collected from male volunteers (n=649, age: 55.7 ± 4.7 years). Two tagging SNPs,CYP27A1 rs4674344andC

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Törzsök, Peter, Jasper Van Goubergen, Martin Pichler, Renate Pichler, and Frédéric R. Santer. "Isochromosome 12p Formation Regulates Vitamin D Metabolism in Testicular Cancer." Nutrients 15, no. 10 (2023): 2384. http://dx.doi.org/10.3390/nu15102384.

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Isochromosome 12p (iChr12p) is typical in almost all invasive testicular cancers. Increased copy number of genes on 12p is associated with the development of a clinically manifest tumor; however, the causative genes have not yet been identified. Chromosome 12 harbors many genes involved in Vitamin D metabolism. RNAseq analysis of Vitamin D receptor (VDR) genes from the TCGA cohort revealed that clustering of VDR expression signatures could differentiate between pure seminomas and non-seminomatous germ cell tumors (NSGCT). Using TCGA mRNA expression of anabolic (CYP2R1, CYP27A1 and CYP27B1) and

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QUINN, Carmel M., Wendy JESSUP, Jenny WONG, Leonard KRITHARIDES та Andrew J. BROWN. "Expression and regulation of sterol 27-hydroxylase (CYP27A1) in human macrophages: a role for RXR and PPARγ ligands". Biochemical Journal 385, № 3 (2005): 823–30. http://dx.doi.org/10.1042/bj20041776.

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CYP27A1 (sterol 27-hydroxylase) catalyses an important sterol elimination pathway in the human macrophage, and consequently may protect against atherosclerosis. We studied the expression and regulation of CYP27A1 in a human macrophage-like cell-line, THP-1, and primary HMDMs (human monocyte-derived macrophages). In both macrophage cell types, we found that CYP27A1 expression is independent of cellular cholesterol levels and of LXR (liver X receptor)-dependent control of transcription. However, the RXR (retinoid X receptor) ligand, 9-cis-retinoic acid, upregulates CYP27A1 expression. Of the RXR

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Petrov, Alexey M., Artem A. Astafev, Natalia Mast, Aicha Saadane, Nicole El-Darzi, and Irina A. Pikuleva. "The Interplay between Retinal Pathways of Cholesterol Output and Its Effects on Mouse Retina." Biomolecules 9, no. 12 (2019): 867. http://dx.doi.org/10.3390/biom9120867.

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In mammalian retina, cholesterol excess is mainly metabolized to oxysterols by cytochromes P450 27A1 (CYP27A1) and 46A1 (CYP46A1) or removed on lipoprotein particles containing apolipoprotein E (APOE). In contrast, esterification by sterol-O-acyltransferase 1 (SOAT) plays only a minor role in this process. Accordingly, retinal cholesterol levels are unchanged in Soat1−/− mice but are increased in Cyp27a1−/−Cyp46a1−/− and Apoe−/− mice. Herein, we characterized Cyp27a1−/−Cyp46a1−/−Soat1−/− and Cyp27a1−/−Cyp46a1−/−Apoe−/− mice. In the former, retinal cholesterol levels, anatomical gross structure

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10

Escher, Geneviève, Isabelle Vögeli, Robert Escher, et al. "Role of CYP27A1 in progesterone metabolism in vitro and in vivo." American Journal of Physiology-Endocrinology and Metabolism 297, no. 4 (2009): E949—E955. http://dx.doi.org/10.1152/ajpendo.00298.2009.

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In the kidney, progesterone is inactivated to 20α-dihydro-progesterone (20α-DH-progesterone) to protect the mineralocorticoid receptor from progesterone excess. In an attempt to clone the enzyme with 20α-hydroxysteroid activity using expression cloning in CHOP cells and a human kidney expression library, serendipitously cDNA encoding CYP27A1 was isolated. Overexpression of CYP27A1 in CHOP cells decreased progesterone conversion to 20α-DH-progesterone in a dose-dependent manner, an effect enhanced by cotransfection with adrenodoxin and adrenodoxin reductase. Incubation of CHOP cells with 27-hyd

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11

Gnedenko, O. V., E. O. Yablokov, P. V. Ershov, et al. "Interaction of prostacyclin synthase with cytochromes P450." Biomeditsinskaya Khimiya 65, no. 1 (2019): 63–66. http://dx.doi.org/10.18097/pbmc20196501063.

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Biosensor experiments on investigation of interaction between prostacyclin synthase (PGIS) and different proteins of the cytochrome P450 monooxygenase systems were perfomed. Interaction of PGIS with microsomal (CYP21A2, CYP2E1) and mitochondrial (CYP27A1, CYP11B1, CYP11B2, CYP11A1) cytochrome P450s was detected. Kinetic and equilibrium parameters of protein complexes formation were determined. Data obtained suggest an essential role of these hemoproteins interaction in regulation of prostacyclin and thromboxane A2 biosynthesis.

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ARAYA, Zufan, Wanjin TANG, and Kjell WIKVALL. "Hormonal regulation of the human sterol 27-hydroxylase gene CYP27A1." Biochemical Journal 372, no. 2 (2003): 529–34. http://dx.doi.org/10.1042/bj20021651.

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The mitochondrial sterol 27-hydroxylase (CYP27A1) is a multifunctional cytochrome P450 enzyme that catalyses important hydroxylations in the biosynthesis of bile acids and bioactivation of vitamin D3. Previous results [Babiker, Andersson, Lund, Xiu, Deeb, Reshef, Leitersdorf, Diczfalusy and Björkhem (1997) J. Biol. Chem. 272, 26253–26261] suggest that CYP27A1 plays an important role in cholesterol homoeostasis and affects atherogenesis. In the present study, the regulation of the human CYP27A1 gene by growth hormone (GH), insulin-like growth factor-1 (IGF-1), dexamethasone, thyroid hormones an

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13

Fang, Ziqi, Guangdong Cheng, Mengting He, and Yanliang Lin. "CYP27A1 deficiency promoted osteoclast differentiation." PeerJ 11 (March 3, 2023): e15041. http://dx.doi.org/10.7717/peerj.15041.

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Background The elevating osteoclast differentiation can lead to an imbalance in bone homeostasis, which was responsible for bone loss and bone diseases, such as osteoporosis. Multiple pathways and molecules have been involved in osteoclast formation, but the role of CYP27A1 in osteoclast differentiation has never been explored. Methods CYP27A1 deficient mice were constructed using CRISPR-Cas9 system. Osteoclast differentiation was detected by TRAP staining. Differentially expressed genes (DEGs) were identified using RNA-seq analysis and were confirmed by qRT-PCR and Western blot. Results The r

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Kaukinen, Antti, Jukka Pelkonen, and Ilkka T. Harvima. "Mast cells express CYP27A1 and CYP27B1 in epithelial skin cancers and psoriasis." European Journal of Dermatology 25, no. 6 (2015): 548–55. http://dx.doi.org/10.1684/ejd.2015.2645.

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Jolliffe, David A., Claire L. Greiller, Charles A. Mein, et al. "Vitamin D receptor genotype influences risk of upper respiratory infection." British Journal of Nutrition 120, no. 8 (2018): 891–900. http://dx.doi.org/10.1017/s000711451800209x.

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AbstractSNP in the vitamin D receptor (VDR) gene is associated with risk of lower respiratory infections. The influence of genetic variation in the vitamin D pathway resulting in susceptibility to upper respiratory infections (URI) has not been investigated. We evaluated the influence of thirty-three SNP in eleven vitamin D pathway genes (DBP, DHCR7, RXRA, CYP2R1, CYP27B1, CYP24A1, CYP3A4, CYP27A1, LRP2, CUBN and VDR) resulting in URI risk in 725 adults in London, UK, using an additive model with adjustment for potential confounders and correction for multiple comparisons. Significant associat

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Mangum, Lee C., Xiang Hou, Abdolsamad Borazjani, Jung Hwa Lee, Matthew K. Ross та J. Allen Crow. "Silencing carboxylesterase 1 in human THP-1 macrophages perturbs genes regulated by PPARγ/RXR and RAR/RXR: down-regulation of CYP27A1–LXRα signaling". Biochemical Journal 475, № 3 (2018): 621–42. http://dx.doi.org/10.1042/bcj20180008.

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Macrophage foam cells store excess cholesterol as cholesteryl esters, which need to be hydrolyzed for cholesterol efflux. We recently reported that silencing expression of carboxylesterase 1 (CES1) in human THP-1 macrophages [CES1KD (THP-1 cells with CES1 expression knocked down) macrophages] reduced cholesterol uptake and decreased expression of CD36 and scavenger receptor-A in cells loaded with acetylated low-density lipoprotein (acLDL). Here, we report that CES1KD macrophages exhibit reduced transcription of cytochrome P45027A1 (CYP27A1) in nonloaded and acLDL-loaded cells. Moreover, levels

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Bevelander, Gideon S., Elsa S. L. C. Pinto, Adelino V. M. Canario, Tom Spanings, and Gert Flik. "CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein." Journal of Endocrinology 196, no. 3 (2007): 625–35. http://dx.doi.org/10.1677/joe-07-0566.

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Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This

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UNO, Yasuhiro, Shinya HOSAKA, and Hiroshi YAMAZAKI. "Identification and Analysis of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 in Cynomolgus Macaques." Journal of Veterinary Medical Science 76, no. 12 (2014): 1647–50. http://dx.doi.org/10.1292/jvms.14-0313.

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Hunsaker, Dustin, James Landon Moore, Katherine M. Howard, and Karl Kingsley. "Vitamin D Receptor and CYP450 Enzyme Dysregulation May Mediate Oral Cancer Responsiveness." Targets 3, no. 1 (2025): 6. https://doi.org/10.3390/targets3010006.

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Many health benefits are associated with Vitamin D (VitD), although deficiency is associated with poor health outcomes and the increased risk of cancer development. For example, many tissue-specific enzymes are involved in VitD metabolism, and mutations or deletions within Vitamin D receptor (VDR) genes are known to increase the cancer risk by altering their functions or bioavailability, although less is known about these phenomena in oral cancers. Using well-characterized, commercially available oral cell lines (OKF4, HGF-1, SCC4, SCC9, SCC15, SCC25, and CAL27), the mRNA expression of P450 cy

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He, Sisi, Liqian Ma, Amy E. Baek, et al. "Host CYP27A1 expression is essential for ovarian cancer progression." Endocrine-Related Cancer 26, no. 7 (2019): 659–75. http://dx.doi.org/10.1530/erc-18-0572.

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There is an urgent need for more effective strategies to treat ovarian cancer. Elevated cholesterol levels are associated with a decreased progression-free survival time (PFS) while statins are protective. 27-Hydroxycholesterol (27HC), a primary metabolite of cholesterol, has been shown to modulate the activities of the estrogen receptors (ERs) and liver x receptors (LXRs) providing a potential mechanistic link between cholesterol and ovarian cancer progression. We found that high expression of CYP27A1, the enzyme responsible for the synthesis of 27HC, was associated with decreased PFS, while

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Vidigal, Verônica Marques, Tiago Donizetti Silva, Juliana de Oliveira, Célia Aparecida Marques Pimenta, Aledson Vitor Felipe, and Nora Manoukian Forones. "Genetic Polymorphisms of Vitamin D Receptor (VDR), CYP27B1 and CYP24A1 Genes and the Risk of Colorectal Cancer." International Journal of Biological Markers 32, no. 2 (2017): 224–30. http://dx.doi.org/10.5301/jbm.5000248.

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Introduction Vitamin D receptor (VDR) and proteins encoded by the genes CYP27B2 and CYP24A1 involved in the production and inactivation of vitamin D can influence vitamin D and the susceptibility to colorectal cancer (CRC). The objective of this study was to investigate the relationship between the risk of CRC and polymorphisms in VDR, CYP27B1 and CYP24A1, lifestyle and dietary habits. Methods The study included 152 patients with CRC and 321 controls. All participants answered a questionnaire on their dietary habits, alcohol consumption and smoking habits. DNA was extracted from peripheral blo

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Ben-Eltriki, Mohamed, Erysa J. Gayle, Jhoanne M. Paras, Louisa Nyame-Addo, Manik Chhabra, and Subrata Deb. "Vitamin D in Melanoma: Potential Role of Cytochrome P450 Enzymes." Life 14, no. 4 (2024): 510. http://dx.doi.org/10.3390/life14040510.

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Vitamin D is a promising anticancer agent for the prevention and treatment of several cancers, including melanoma. Low 25-hydroxyvitamin D levels, a routinely used marker for vitamin D, have been suggested as one of the factors in the development and progression of melanoma. The parent vitamin D needs activation by cytochrome P450 (CYP) enzymes to exert its actions via the vitamin D receptor (VDR). This review discusses the role of vitamin D in melanoma and how CYP-mediated metabolism can potentially affect the actions of vitamin D. Through interacting with the retinoid X receptor, VDR signali

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Agnello, Luisa, Concetta Scazzone, Bruna Lo Sasso та ін. "CYP27A1, CYP24A1, and RXR-α Polymorphisms, Vitamin D, and Multiple Sclerosis: a Pilot Study". Journal of Molecular Neuroscience 66, № 1 (2018): 77–84. http://dx.doi.org/10.1007/s12031-018-1152-9.

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Bonnet, Lauriane, Esma Karkeni, Charlène Couturier, et al. "Four days high fat diet modulates vitamin D metabolite levels and enzymes in mice." Journal of Endocrinology 248, no. 1 (2021): 87–93. http://dx.doi.org/10.1530/joe-20-0198.

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Obesity is classically associated with low serum total and free 25(OH)D. Hypotheses have been advanced to explain this observation but mechanisms remain poorly understood, and notably priming events that could explain such association. We investigated the impact of short-term high fat (HF) diet to investigate early events occurring in vitamin D metabolism. Male C57BL/6J mice were fed with a control diet (control group) and HF diet for 4 days. HF fed mice displayed similar body weight to control mice but significantly increased adiposity, together with a decrease of free 25(OH)D concentrations,

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Vidigal, Veronica Marques, Pedro Nazareth Aguiar, Tiago Donizetti Silva, et al. "Genetic Polymorphisms of Vitamin D Metabolism Genes and Serum Level of Vitamin D in Colorectal Cancer." International Journal of Biological Markers 32, no. 4 (2017): 441–46. http://dx.doi.org/10.5301/ijbm.5000282.

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Background The metabolism of vitamin D is complex, its receptor (VDR) and proteins encoded by the genes CYP27B2 and CYP24A1 can influence vitamin D serum levels. The aim of this study was to investigate the relationship of the polymorphisms of VDR (ApaI and BsmI), CYP27B1 and CYP24A1 with serum vitamin D levels in both forms, 25(OH)D3 (circulating form) and 1,25(OH)2D3 (active form), in colorectal cancer (CRC) patients. Methods One hundred fifty-two CRC patients and 321 controls were included. DNA was extracted from peripheral blood. Polymorphisms of BsmI and ApaI were identified by PCR-RFLP.

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Thanoon, Asmaa Y., and Faehaa Azher Al Mashhadane. "Role of 1,25(OH)2D On Cytochromes CYP27A1 and CYP27B1 in Periodontitis: A Clinical Study." Pharmacognosy Journal 15, no. 6 (2024): 1112–15. http://dx.doi.org/10.5530/pj.2023.15.202.

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Bhoora, Sachin, Tahir S. Pillay, and Rivak Punchoo. "Cholecalciferol Mediates Apoptosis in Siha Cervical Cancer Line via Autocrine Mechanisms." Journal of the Endocrine Society 5, Supplement_1 (2021): A1013. http://dx.doi.org/10.1210/jendso/bvab048.2072.

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Abstract Cervical cancer disproportionately affects low-resource countries and is a significant health burden in South Africa. Pre-clinical studies have demonstrated numerous anti-cancer actions of vitamin D metabolites. Here, the anti-cancer action of the vitamin D precursor, cholecalciferol, was investigated in a high-grade cervical cancer cell line, SiHa. SiHa cell cultures were treated with a range of cholecalciferol doses (26 nM, 104 nM, 260 nM and 2600 nM) for 72 hours. Cell count and viability were assessed by crystal violet and trypan blue assays, respectively. Apoptotic cell death was

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Ehrhardt, Maximilian, Adrian Gerber, Josef Zapp, Frank Hannemann та Rita Bernhardt. "Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol". Biological Chemistry 397, № 6 (2016): 513–18. http://dx.doi.org/10.1515/hsz-2016-0111.

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Abstract β-Sitosterol and ergosterol are the equivalents of cholesterol in plants and fungi, respectively, and common sterols in the human diet. In the current work, both were identified as novel CYP27A1 substrates by in vitro experiments applying purified human CYP27A1 and its redox partners adrenodoxin (Adx) and adrenodoxin reductase (AdR). A Bacillus megaterium based biocatalyst recombinantly expressing the same proteins was utilized for the conversion of the substrates to obtain sufficient amounts of the novel products for a structural NMR analysis. β-Sitosterol was found to be converted i

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Liu, Lingyu, Janak L. Pathak, Yong-qiang Zhu, and Matthias Bureik. "Comparison of cytochrome P450 expression in four different human osteoblast models." Biological Chemistry 398, no. 12 (2017): 1327–34. http://dx.doi.org/10.1515/hsz-2017-0205.

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AbstractCytochromes P450 (CYPs) are important for bone homeostasis, but only limited information is available on their expression in human bone cells. We analyzed the expression levels of eight CYPs in osteoblasts cultured in human bone pieces, in osteoblasts differentiated from human periosteum mesenchymal stem cells, in primary human osteoblasts and in the human osteoblast cell line MG63, respectively. Our results confirm previous reports about the presence of CYP11A1, CYP17A1, CYP24A1 and CYP27B1, while demonstrating expression of CYP2E1, CYP26A1, CYP39A1 and CYP51A1 for the first time. How

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Wang, Lijing, Cui Zhou, Huiyan Yu, et al. "Vitamin D, Folic Acid and Vitamin B12 Can Reverse Vitamin D Deficiency-Induced Learning and Memory Impairment by Altering 27-Hydroxycholesterol and S-Adenosylmethionine." Nutrients 15, no. 1 (2022): 132. http://dx.doi.org/10.3390/nu15010132.

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The cholesterol-oxidized metabolite 27-hydroxycholesterol (27-OHC) is synthesized by CYP27A1, which is a key factor in vitamin D and oxysterol metabolism. Both vitamin D and 27-OHC are considered to play important roles in Alzheimer’s disease (AD). The study aims to research the effects of co-supplementation of vitamin D, folic acid, and vitamin B12 on learning and memory ability in vitamin D-deficient mice, and to explore the underlying mechanism. In this study, C57BL/6J mice were fed a vitamin D-deficient diet for 13 weeks to establish a vitamin D-deficient mice model. The vitamin D-deficien

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Avalos-de León, Cindy G., Mónica B. Jiménez-Castro, María Eugenia Cornide-Petronio, et al. "The Effect of Fibroblast Growth Factor 15 Signaling in Non-Steatotic and Steatotic Liver Transplantation from Cardiocirculatory Death." Cells 8, no. 12 (2019): 1640. http://dx.doi.org/10.3390/cells8121640.

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We elucidate the relevance of fibroblast growth factor 15 (FGF15) in liver transplantation (LT) using rats with both steatotic and non-steatotic organs from donors after cardiocirculatory death (DCD). Compared to LT from non-DCDs, the induction of cardiocirculatory death (CD) increases hepatic damage, proliferation, and intestinal and circulatory FGF15. This is associated with high levels of FGF15, bilirubin and bile acids (BAs), and overexpression of the enzyme involved in the alternative BA synthesis pathway, CYP27A1, in non-steatotic livers. Furthermore, CD activates the proliferative pathw

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Norlin, Maria, Sara von Bahr, Ingemar Björkhem, and Kjell Wikvall. "On the substrate specificity of human CYP27A1." Journal of Lipid Research 44, no. 8 (2003): 1515–22. http://dx.doi.org/10.1194/jlr.m300047-jlr200.

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Sawada, Natsumi, Toshiyuki Sakaki, Miho Ohta, and Kuniyo Inouye. "Metabolism of Vitamin D3 by Human CYP27A1." Biochemical and Biophysical Research Communications 273, no. 3 (2000): 977–84. http://dx.doi.org/10.1006/bbrc.2000.3050.

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Horváth, Evelin, Bernadett Balla, János Kósa, et al. "A D-vitamin metabolizmusa humán hepatocellularis carcinomában és az azt körülvevő tumormentes májszövetben." Orvosi Hetilap 157, no. 48 (2016): 1910–18. http://dx.doi.org/10.1556/650.2016.30592.

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Introduction: 1,25-Dihydroxy vitamin D3 mediates antitumor effects in hepatocellular carcinoma. Aim: We examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin D3-inactivating CYP24A1, mRNA of activating CYP27B1 enzymes, and that of VDR between human hepatocellular carcinoma and surrounding non-tumorous liver. Methods: Snap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunoh

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Wooton-Kee, Clavia Ruth, David E. Cohen та Mary Vore. "Increased cholesterol 7α-hydroxylase expression and size of the bile acid pool in the lactating rat". American Journal of Physiology-Gastrointestinal and Liver Physiology 294, № 4 (2008): G1009—G1016. http://dx.doi.org/10.1152/ajpgi.00017.2008.

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Maximal bile acid secretory rates and expression of bile acid transporters in liver and ileum are increased in lactation, possibly to facilitate increased enterohepatic recirculation of bile acids. We determined changes in the size and composition of the bile acid pool and key enzymes of the bile acid synthetic pathway [cholesterol 7α-hydroxylase (Cyp7a1), sterol 27-hydroxylase (Cyp27a1), and sterol 12α-hydroxylase (Cyp8b1)] in lactating rats relative to female virgin controls. The bile acid pool increased 1.9 to 2.5-fold [postpartum (PP) days 10, 14, and 19–23], compared with controls. A 1.5-

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Kamble, Nitin, Vishwanatha R. A. P. Reddy, Ben Jackson, et al. "Inhibition of Marek’s Disease Virus Replication and Spread by 25-hydroxycholesterol and 27-hydroxycholesterol In Vitro." Viruses 15, no. 8 (2023): 1652. http://dx.doi.org/10.3390/v15081652.

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Marek’s disease virus (MDV) causes a deadly lymphoproliferative disease in chickens, resulting in huge economic losses in the poultry industry. It has been suggested that MDV suppresses the induction of type I interferons and thus escapes immune control. Cholesterol 25-hydroxylase (CH25H), a gene that encodes an enzyme that catalyses cholesterol to 25-hydroxycholesterol (25-HC), is an interferon-stimulating gene (ISG) known to exert antiviral activities. Other oxysterols, such as 27-hydroxycholesterols (27-HC), have also been shown to exert antiviral activities, and 27-HC is synthesised by the

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Ishizawa, Michiyasu, Ken-ichi Iwasaki, Shigeaki Kato, and Makoto Makishima. "Hypergravity modulates vitamin D receptor target gene mRNA expression in mice." American Journal of Physiology-Endocrinology and Metabolism 297, no. 3 (2009): E728—E734. http://dx.doi.org/10.1152/ajpendo.00168.2009.

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The possibility of pathological calcium metabolism is a critical health concern introduced by long-term space travel. Because vitamin D plays an important role in calcium homeostasis, we evaluated the effects of hypergravity on the expression of genes involved in vitamin D and calcium metabolism in ICR mice. When exposed to 2G hypergravity for 2 days, the mRNA expression of renal 25-hydroxyvitamin D 24-hydroxylase (Cyp24a1) was increased and that of 25-hydroxyvitamin D 1α-hydroxylase (Cyp27b1) was decreased. Although hypergravity decreased food intake and increased the expression of starvation

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Alfaqih, Mahmoud A., Erik R. Nelson, Wen Liu, et al. "CYP27A1 Loss Dysregulates Cholesterol Homeostasis in Prostate Cancer." Cancer Research 77, no. 7 (2017): 1662–73. http://dx.doi.org/10.1158/0008-5472.can-16-2738.

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Pikuleva, Irina, and Norman B. Javitt. "Novel sterols synthesized via the CYP27A1 metabolic pathway." Archives of Biochemistry and Biophysics 420, no. 1 (2003): 35–39. http://dx.doi.org/10.1016/j.abb.2003.09.028.

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Jiang, Yi, Liyan Liao, Jina Li, Larry Wang, and Zhongjian Xie. "Older Age Is Associated with Decreased Levels of VDR, CYP27B1, and CYP24A1 and Increased Levels of PTH in Human Parathyroid Glands." International Journal of Endocrinology 2020 (April 9, 2020): 1–6. http://dx.doi.org/10.1155/2020/7257913.

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Parathyroid glands contain the vitamin D receptor (VDR) and 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1), which catalyze the production and degradation of 1,25-dihydroxyvitamin D [1,25(OH)2D], respectively. Previous studies have shown that the serum level of intact parathyroid hormone (iPTH) increases with age. We hypothesized that the expression of CYP27B1 or VDR in parathyroid glands decreases with age, which might account for the increased serum levels of iPTH due to decreased suppression of parathyroid hormone (PTH) secretion by 1,25(OH)2D in older people. To t

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Abouzid, Mohamed, Marta Karaźniewicz-Łada, Basel Abdelazeem, and James Robert Brašić. "Research Trends of Vitamin D Metabolism Gene Polymorphisms Based on a Bibliometric Investigation." Genes 14, no. 1 (2023): 215. http://dx.doi.org/10.3390/genes14010215.

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Vitamin D requires activation to show its pharmacological effect. While most studies investigate the association between vitamin D and disease, only a few focus on the impact of vitamin D metabolism gene polymorphisms (vitDMGPs). This bibliometric study aims to provide an overview of current publications on vitDMGPs (CYP27B1, CYP24A1, CYP2R1, CYP27A1, CYP2R1, DHCR7/NADSYN1), compare them across countries, affiliations, and journals, and inspect keywords, co-citations, and citation bursts to identify trends in this research field. CiteSpace© (version 6.1.R3, Chaomei Chen), Bibliometrix© (R vers

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Slominski, Andrzej, Tae-Kang Kim, Radomir Slominski та ін. "RF15 | PMON293 Metabolic activation of tachysterol to biologically active hydroxyderivatives that act on VDR, AhR, LXRs and PPARγ receptors". Journal of the Endocrine Society 6, Supplement_1 (2022): A734. http://dx.doi.org/10.1210/jendso/bvac150.1515.

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Abstract Absorption of ultraviolet B (UVB) radiation by the B ring of 7-dehydrocholesterol (7-DHC) leads to the transformation of 7-DHC to previtamin D3, which after absorption of additional UVB isomerizes to tachysterol3 (T3) and lumisterol3 (L3). Previously we demonstrated that CYP11A1 can hydroxylate the side chain of vitamin D3 (D3), 7-DHC and L3. Similarly CYP27A1 can hydroxylate the side chain of L3 to biologically active hydroxyderivatives. In a continuation of these studies, we report that CYP11A1 and CYP27A1 hydroxylate T3 to 20S(OH)T3 and 25(OH)T3, respectively, plus minor unidentifi

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Oguro, Hideyuki, Jeffrey McDonald, and Sean Morrison. "27-Hydroxycholesterol Induces Hematopoietic Stem Cell Mobilization and Extramedullary Hematopoiesis Mediated By Estrogen Receptor Alpha." Blood 130, Suppl_1 (2017): 636. http://dx.doi.org/10.1182/blood.v130.suppl_1.636.636.

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Abstract In adults, hematopoietic stem cells (HSCs) reside primarily in the bone marrow and their number is tightly regulated under steady state conditions. However, acute demands on the hematopoietic system promote HSC division and mobilization to extramedullary tissues such as the spleen, to increase production of blood cells. While the mechanisms that regulate HSC numbers and residence in the bone marrow under steady-state conditions have been extensively characterized, the mechanisms that activate HSCs in response to acute hematopoietic demands are less well understood. We have previously

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Araya, Zufan, Fardin Hosseinpour, Karl Bodin, and Kjell Wikvall. "Metabolism of 25-hydroxyvitamin D3 by microsomal and mitochondrial vitamin D3 25-hydroxylases (CYP2D25 and CYP27A1): a novel reaction by CYP27A1." Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1632, no. 1-3 (2003): 40–47. http://dx.doi.org/10.1016/s1388-1981(03)00062-3.

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Fam, Marina Sherif, Sally I. Hassanein, Mohamed Farouk Abdel Rahman, Reem Amr Assal, Rasha Sayed Hanafi, and Mohamed Zakaria Gad. "Contribution of CYP27B1 and CYP24A1 genetic variations to the incidence of acute coronary syndrome and to vitamin D serum level." Canadian Journal of Physiology and Pharmacology 97, no. 12 (2019): 1152–58. http://dx.doi.org/10.1139/cjpp-2019-0258.

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Cardiovascular diseases remain a major public health burden worldwide. It was reported that vitamin D protects the cardiovascular system through several mechanisms mainly by hindering atherosclerosis development. Genetic variations in vitamin D metabolic pathway were found to affect vitamin D levels. This study aimed at investigating the association between single nucleotide polymorphisms in genes involved in vitamin D metabolism, CYP27B and CYP24A1; 25-hydroxyvitamin D (25(OH)D) levels; and susceptibility to acute coronary syndrome (ACS). One hundred and eighty-five patients and 138 healthy c

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Ma, Rong, Yang Gu, Shuang Zhao, Jingxia Sun, Lynn J. Groome, and Yuping Wang. "Expressions of vitamin D metabolic components VDBP, CYP2R1, CYP27B1, CYP24A1, and VDR in placentas from normal and preeclamptic pregnancies." American Journal of Physiology-Endocrinology and Metabolism 303, no. 7 (2012): E928—E935. http://dx.doi.org/10.1152/ajpendo.00279.2012.

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Vitamin D insufficiency/deficiency during pregnancy has been linked to increased risk of preeclampsia. Placenta dysfunction plays an important role in the pathogenesis of this pregnancy disorder. In this study, we tested the hypothesis that disturbed vitamin D metabolism takes place in preeclamptic placentas. Protein expressions of vitamin D binding protein (VDBP), 25-hydroxylase (CYP2R1), 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1), and vitamin D receptor (VDR) were examined in placentas from normotensive and preeclamptic pregnancies. By immunostaining we found that in normal placenta

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Escher, Genevieve, Zygmunt Krozowski, Kevin D. Croft, and Dmitri Sviridov. "Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux." Journal of Biological Chemistry 278, no. 13 (2003): 11015–19. http://dx.doi.org/10.1074/jbc.m212780200.

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Amararathna, Madumani, David W. Hoskin, Kerry B. Goralski, and H. P. Vasantha Rupasinghe. "Suppression of NNK Metabolism by Anthocyanin-Rich Haskap Berry Supplementation Through Modulation of P450 Enzymes." Pharmaceuticals 17, no. 12 (2024): 1615. https://doi.org/10.3390/ph17121615.

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Oral supplementation of anthocyanins-rich haskap (Lonicera caerulea) berry (HB) reduces 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis, cytotoxicity, DNA damage, and modulated inflammation in vitro and in vivo. The procarcinogen NNK is metabolically activated by cytochrome P450 (P450) enzymes, producing reactive metabolites that induce lung carcinogenesis. Hypothesis: Therefore, we hypothesized that the HB-modulated protective effect against NNK could be due to its ability to suppress P450 enzymes. Methods: HB (6 mg of cyanidin-3-O-glucoside [C3G] in 0.2 g of H

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VON WEYMARN, L. B., and S. E. MURPHY. "CYP2A13-catalysed coumarin metabolism: comparison with CYP2A5 and CYP2A6." Xenobiotica 33, no. 1 (2003): 73–81. http://dx.doi.org/10.1080/0049825021000022302.

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Yabut, King Clyde B., and Nina Isoherranen. "CRABPs Alter all-trans-Retinoic Acid Metabolism by CYP26A1 via Protein-Protein Interactions." Nutrients 14, no. 9 (2022): 1784. http://dx.doi.org/10.3390/nu14091784.

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Cellular retinoic acid binding proteins (CRABP1 and CRABP2) bind all-trans-retinoic acid (atRA), the active metabolite of vitamin A, with high affinity. CRABP1 and CRABP2 have been shown to interact with the atRA-clearing cytochrome P450 enzymes CYP26B1 and CYP26C1 and with nuclear retinoic acid receptors (RARs). We hypothesized that CRABP1 and CRABP2 also alter atRA metabolism and clearance by CYP26A1, the third key atRA-metabolizing enzyme in the CYP26 family. Based on stopped-flow experiments, atRA bound CRABP1 and CRABP2 with Kd values of 4.7 nM and 7.6 nM, respectively. The unbound atRA K

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