Academic literature on the topic 'Cytochrome P450'

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Journal articles on the topic "Cytochrome P450"

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Bandiera, S. "Expression and catalysis of sex-specific cytochrome P450 isozymes in rat liver." Canadian Journal of Physiology and Pharmacology 68, no. 6 (1990): 762–68. http://dx.doi.org/10.1139/y90-117.

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Research interest in the study of cytochromes P450 has recently been shifting to the characterization of "constitutively" expressed isozymes from that of the inducible forms. Several "constitutive" cytochrome P450 isozymes have been purified from rat liver including five immunochemically related proteins designated cytochromes P450f, P450g, P450h, P450i, and P450k. These hemoproteins have been identified as distinct isozymes on the basis of spectral, electrophoretic, and catalytic properties and NH2-terminal sequence analysis. Purification and immunoquantitation studies have indicated that the
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Ershov, P. V., Yu V. Mezentsev, E. O. Yablokov, et al. "Study specificity of isatin interactions with P450 cytochromes." Biomeditsinskaya Khimiya 64, no. 1 (2018): 61–65. http://dx.doi.org/10.18097/pbmc20186401061.

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Cytochrome P450-dependent monooxygenase systems exist basically in all living organisms, where they perform various important functions. The coordinated functioning of these systems involves many proteins participating in different protein-protein interactions (PPI). Previously, we have found that the endogenous non-peptide bioregulator isatin (indoledione-2,3), synthesized from indole by means of certain cytochromes P450 (e.g. P450 2E1, P450 2C19, P450 2A6) regulates affinity of some PPI. In this work, an attempt has been undertaken to register a direct interaction of isatin with a set of dif
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Godbole, Rucha C., Anupama A. Pable, and Vitthal T. Barvkar. "Transcriptome-wide identification, characterization, and phylogenomic analysis of cytochrome P450s from Nothapodytes nimmoniana reveal candidate genes involved in the camptothecin biosynthetic pathway." Genome 64, no. 1 (2021): 1–14. http://dx.doi.org/10.1139/gen-2020-0067.

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The plant Nothapodytes nimmoniana is an important source of camptothecin (CPT), an anticancer compound widely used in the treatment of colorectal, lung, and ovarian cancers. CPT is biosynthesized by the combination of the seco-iridoid and indole pathways in plants. The majority of the biosynthetic steps and associated genes still remain unknown. Certain reactions in the seco-iridoid pathway are catalyzed by cytochrome P450 enzymes. Hence, identifying transcriptionally active cytochrome P450 genes becomes essential in the elucidation of the CPT biosynthetic pathway. Here, we report the identifi
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Iwasaki, M., R. L. P. Lindberg, R. O. Juvonen та M. Negishi. "Site-directed mutagenesis of mouse steroid 7α-hydroxylase (cytochrome P-4507α): role of residue-209 in determining steroid-cytochrome P-450 interaction". Biochemical Journal 291, № 2 (1993): 569–73. http://dx.doi.org/10.1042/bj2910569.

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We have cloned a cDNA encoding mouse steroid 7 alpha-hydroxylase P450(7) alpha (cytochrome P-450(7) alpha) and expressed it in Saccharomyces cerevisiae. Mouse P450(7) alpha is 70% identical in its amino acid sequence with the mouse steroid 15 alpha-hydroxylase P450(15) alpha (2A4). The Leu at position 209 of P450(15) alpha is the most important residue to determine the steroid hydroxylase activity of the P450 [Lindberg and Negishi (1989) Nature (London) 339, 632-634]. The P450(7) alpha contains Asn at the position corresponding to the Leu-209 of P450(15) alpha, although both P450s hydroxylate
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Hodek, Petr, Tomáš Koblas, Helena Rýdlová, et al. "Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450." Collection of Czechoslovak Chemical Communications 69, no. 3 (2004): 659–73. http://dx.doi.org/10.1135/cccc20040659.

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Using chicken antibodies IgY (purified from egg yolks) against mammalian cytochromes P450 and by means of cytochrome P450 marker substrates, we found for the first time the presence of hepatopancreatic cytochrome P450 in crayfishOrconectes limosus(an inducible cytochrome P450 2B-like enzyme) and we were able to detect and quantify cytochrome P450 1A1 in microsomes of human livers. Expression levels of cytochrome P450 1A1 in human livers constituted less than 0.6% of the total hepatic cytochrome P450 complement. The results obtained in our study are clear examples that chicken IgY are suitable
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Rwere, Freeborn, Sangchoul Im, and Lucy Waskell. "The FMN “140s Loop” of Cytochrome P450 Reductase Controls Electron Transfer to Cytochrome P450." International Journal of Molecular Sciences 22, no. 19 (2021): 10625. http://dx.doi.org/10.3390/ijms221910625.

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Cytochrome P450 reductase (CYPOR) provides electrons to all human microsomal cytochrome P450s (cyt P450s). The length and sequence of the “140s” FMN binding loop of CYPOR has been shown to be a key determinant of its redox potential and activity with cyt P450s. Shortening the “140s loop” by deleting glycine-141(ΔGly141) and by engineering a second mutant that mimics flavo-cytochrome P450 BM3 (ΔGly141/Glu142Asn) resulted in mutants that formed an unstable anionic semiquinone. In an attempt to understand the molecular basis of the inability of these mutants to support activity with cyt P450, we
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Munro, A. W., K. J. McLean, K. R. Marshall, et al. "Cytochromes P450: novel drug targets in the war against multidrug-resistant Mycobacterium tuberculosis." Biochemical Society Transactions 31, no. 3 (2003): 625–30. http://dx.doi.org/10.1042/bst0310625.

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Novel drug strategies are desperately needed to combat the global threat posed by multidrug-resistant strains of Mycobacterium tuberculosis (Mtb). The genome sequence of Mtb has revealed an unprecedented number of cytochrome P450 enzymes in a prokaryote, suggesting fundamental physiological roles for many of these enzymes. Several azole drugs (known inhibitors of cytochromes P450) have been shown to have potent anti-mycobacterial activity, and the most effective azoles have extremely tight binding constants for one of the Mtb P450s (CYP121). The structure of CYP121 has been determined at atomi
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Hrubý, Kamil, Eva Anzenbacherová, Pavel Anzenbacher, and Milan Nobilis. "Biotransformation of Benfluron by Rat Hepatic Cytochrome P450. Identification of Individual CYP-Enzymes Involved in Biotransformation of Benfluron, Prospective Antineoplastic Based on Benzo[c]fluorene." Collection of Czechoslovak Chemical Communications 65, no. 8 (2000): 1374–86. http://dx.doi.org/10.1135/cccc20001374.

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Benfluron, 5-[2-(dimethylamino)ethoxy]-7H-benzo[c]fluoren-7-one hydrochloride, a prospective antineoplastic agent, is metabolised by cytochromes P450 to N-demethyl and 9-hydroxy derivatives. To prove the participation of individual cytochrome P450 isoforms in formation of these metabolites, selective induction of cytochromes P450, inhibition of benfluron biotransformation using inhibitors specific for individual cytochromes P450, and inhibition by benfluron of "marker" enzyme activities characteristic of certain cytochromes P450 were used. N-Demethylbenfluron appears to be formed mainly by the
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Shumyantseva, Victoria V., Tatiana V. Bulko, Polina I. Koroleva, et al. "Human Cytochrome P450 2C9 and Its Polymorphic Modifications: Electroanalysis, Catalytic Properties, and Approaches to the Regulation of Enzymatic Activity." Processes 10, no. 2 (2022): 383. http://dx.doi.org/10.3390/pr10020383.

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The electrochemical properties of cytochrome P450 2C9 (CYP2C9) and polymorphic modifications P450 2C9*2 (CYP2C9*2) and P450 2C9*3 (CYP2C9*3) were studied. To analyze the comparative electrochemical and electrocatalytic activity, the enzymes were immobilized on electrodes modified with a membrane-like synthetic surfactant (didodecyldimethylammonium bromide (DDAB)). An adequate choice of the type of modified electrode was confirmed by cyclic voltammetry of cytochromes P450 under anaerobic conditions, demonstrating well-defined peaks of reduction and oxidation of the heme iron. The midpoint poten
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Pratiwi, R. A., N. S. W. Yahya, and Y. Chi. "Bio function of Cytochrome P450 on fungus: a review." IOP Conference Series: Earth and Environmental Science 959, no. 1 (2022): 012023. http://dx.doi.org/10.1088/1755-1315/959/1/012023.

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Abstract Cytochrome P450 is the superfamily of proteins involved in the metabolism of organisms, including fungi. Fungal have more diverse P450 families than plants, animals, or bacteria. Research on fungal P450 has blossomed and become an important area in biology and ecology. Cytochrome P450 could be detoxifying natural and environmental contaminants to survive in several ecological niches. Furthermore, the presence of the fungal Cytochrome P450 as an antifungal drug target is a promising approach for the controlling of pest and plant pathogenic fungi. To date, numerous studies have revealed
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Dissertations / Theses on the topic "Cytochrome P450"

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Skinner, Michael Stephen. "Olfactory cytochrome P450." Thesis, University of Warwick, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307349.

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Farooq, Yassar. "Mechanisms of electron transfer from cytochrome P450 reductase to cytochrome P450 3A4." Thesis, University of Leicester, 2010. http://hdl.handle.net/2381/8597.

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The study demonstrates that CPR and P450 3A4 can be prepared to highly pure state by the use of detergent CHAPS. Optimisation of published methods led to pure flavoprotein, ~90% full-length with a small amount of truncated CPR. The reconstitution of CPR and P450 3A4 into liposomes using CHAPS and Superose 6 column purification has achieved a homogenous highly functional proteoliposomes with good catalytic activity and almost completely reducible P450 3A4 in a simple controllable system. The spectroscopic data has shown that reduction of P450 3A4 in proteoliposomes was at least 10 fold higher t
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Al-Anizy, Mohammed. "Studies on cytochrome P450 4X1." Thesis, University of Nottingham, 2005. http://eprints.nottingham.ac.uk/10404/.

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Antisera raised against recombinant human CYP4Z1 (4Z1), mouse Aryl hydrocarbon receptor (AhR), and C.elegans Latrophilin (LpH) proteins were titred over the course of several bleeds. The sensitivity of the antibodies shows an increase over the course of several immunizations, with the minimum amount detected being 1 ng of 4Z1, 0.3 ng of AhR, and 0.3 ng of LpH. Terminal bleeds were taken for the AhR and LpH antisera. The AhR antisera detects proteins in rat and mouse liver cytosol consistent with previous reports of the AhR. LpH is predicted to be localized in a membrane compartment on the basi
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Bell, Stephen Graham. "The use of active site mutants of cytochrome P450(cam) in chemical synthesis." Thesis, University of Oxford, 1999. http://ora.ox.ac.uk/objects/uuid:7f48cf79-37b0-45cd-a40e-e971af466cff.

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This thesis describes a study of the substrate selectivity of active site mutants of the monooxygenase cytochrome P450<sub>cam</sub>. A range of mutants was constructed which replaced the phenolic side-chain at the Tyr-96 position by various hydrophobic amino acid residues. These 'hydrophobic mutants' were then combined with other mutations around the active site (Val-247, Phe-87, Ile-395 and Phe-193) which altered the space available at different positions in the active site. These mutants were then tested with an in vitro reconstituted P450<sub>cam</sub> system with a range of substrates rel
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Sideri, Anastasia. "Directed evolution of cytochrome P450 BM3." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435931.

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Marsh, Rachael. "Cytochrome P450 studies in Aspergillus fumigatus." Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364267.

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Maughan, Juanita Amanda. "Molecular investigations of plant cytochrome P450." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388204.

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Papagiannidou, Eleni. "Cytochrome P450-mediated metabolism of melatonin." Thesis, University of Surrey, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422928.

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Tyzack, Jonathan David. "Prediction of cytochrome P450 xenobiotic metabolism." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708289.

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Busi, Florent. "Pharmacogénétique du cytochrome P450 humain CYP3A5." Paris 5, 2005. http://www.theses.fr/2005PA05P621.

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Les CYP3A sont les P450 les plus abondants dans le foie où ils métabolisent plus de 50 % des médicaments. Le CYP3A5 n'est exprimé que dans 25 % de la population caucasienne. Une mutation (CYP3A5*3) entraînant un épissage alternatif a été associée à un faible niveau de CYP3A5. Un niveau d'ARNm CYP3A5 plus élevé chez les individus *1/*3 par rapport aux homozygotes *3/*3 nous a amené à rechercher les causes de cette variation. L'ARN *3 est plus instable et sa dégradation est réalisée par la NMD (nonsense mediated mRNA decay). Le génotype CYP3A5 a des conséquences en clinique. Nous avons mis au po
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Books on the topic "Cytochrome P450"

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Paul R. Ortiz de Montellano, Ian R. Phillips, and Elizabeth A. Shephard. Cytochrome P450 protocols. 3rd ed. Humana, 2013.

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1951-, Phillips Ian R., and Shephard Elizabeth A. 1950-, eds. Cytochrome P450 protocols. 2nd ed. Humana Press, 2005.

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Yan, Zhengyin, and Gary W. Caldwell, eds. Cytochrome P450. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1542-3.

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Schenkman, John B., and Helmut Greim, eds. Cytochrome P450. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77763-9.

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Ortiz de Montellano, Paul R., ed. Cytochrome P450. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-12108-6.

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Ortiz de Montellano, Paul R., ed. Cytochrome P450. Springer US, 2005. http://dx.doi.org/10.1007/b139087.

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de Montellano, Paul R. Ortiz, ed. Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5.

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F, Johnson Eric, and Waterman Michael R, eds. Cytochrome P450. Academic Press, 1996.

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Emel, Arinç, Schenkman John B, and Greim Helmut, eds. Cytochrome P450. Springer-Verlag, 1993.

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R, Waterman Michael, and Johnson Eric F, eds. Cytochrome P450. Academic Press, 1991.

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Book chapters on the topic "Cytochrome P450"

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Girhard, Marco, Patrick J. Bakkes, Osama Mahmoud, and Vlada B. Urlacher. "P450 Biotechnology." In Cytochrome P450. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-12108-6_8.

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Groves, John T., and Yuan-Zhang Han. "Models and Mechanisms of Cytochrome P450 Action." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_1.

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Whitlock, James P., and Michael S. Denison. "Induction of Cytochrome P450 Enzymes That Metabolize Xenobiotics." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_10.

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Waxman, David J., and Thomas K. H. Chang. "Hormonal Regulation of Liver Cytochrome P450 Enzymes." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_11.

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Kagawa, Norio, and Michael R. Waterman. "Regulation of Steroidogenic and Related P450s." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_12.

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Capdevila, Jorge H., Darryl Zeldin, Keiko Makita, Armando Karara, and John R. Falck. "Cytochrome P450 and the Metabolism of Arachidonic Acid and Oxygenated Eicosanoids." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_13.

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Guengerich, F. Peter. "Human Cytochrome P450 Enzymes." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_14.

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Mansuy, Daniel, and Jean-Paul Renaud. "Heme-Thiolate Proteins Different from Cytochromes P450 Catalyzing Monooxygenations." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_15.

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Marnett, Lawrence J., and Todd A. Kennedy. "Comparison of the Peroxidase Activity of Hemoproteins and Cytochrome P450." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_2.

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Mueller, Ernest J., Paul J. Loida, and Stephen G. Sligar. "Twenty-five Years of P450cam Research." In Cytochrome P450. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-2391-5_3.

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Conference papers on the topic "Cytochrome P450"

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Burris-Hiday, Sarah, Mengqi Chai, Michael L. Gross, and Emily Scott. "Human Cytochrome P450 Interactions with Redox Partner Cytochrome P450 Reductase." In ASPET 2023 Annual Meeting Abstracts. American Society for Pharmacology and Experimental Therapeutics, 2023. http://dx.doi.org/10.1124/jpet.122.267650.

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"Cytochrome P450 Enzymes and Microbial Drug Preparation." In 2017 International Conference on Materials Science and Biological Engineering. Francis Academic Press, 2017. http://dx.doi.org/10.25236/icmsbe.2017.14.

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Seguin, Ryan P., Libin Xu, and Ryan Nguyen. "Unusual Metabolism of Benzethonium by Cytochrome P450." In ASPET 2023 Annual Meeting Abstracts. American Society for Pharmacology and Experimental Therapeutics, 2023. http://dx.doi.org/10.1124/jpet.122.564180.

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Tian, Haijin, Paul Quehl, Joel Hollender, and Joachim Jose. "Surface display of human cytochrome P450 enzymes 3A4, 1A2, 2C9, 2C19 and 2D6 with cytochrome P450 reductase for drug metabolism studies." In 5th International Electronic Conference on Medicinal Chemistry. MDPI, 2019. http://dx.doi.org/10.3390/ecmc2019-06333.

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Özgen, İlker Tolga, Esra Kutlu, Hatice Nursoy, Yaşar Cesur, and Gözde Yeşil. "P94 Cytochrome P450 oxidoreductase enzyme deficiency: a case report." In Faculty of Paediatrics of the Royal College of Physicians of Ireland, 9th Europaediatrics Congress, 13–15 June, Dublin, Ireland 2019. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2019. http://dx.doi.org/10.1136/archdischild-2019-epa.449.

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Баранова, Надежда Ивановна, Людмила Андреевна Ащина, and Александра Игоревна Болгова. "ROLE OF CYTOKINES AND CYTOCHROMES P450 IN METABOLISM OF ANTIVIRAL DRUGS FROM COVID-19." In Вопросы фундаментальных и прикладных научных исследований: сборник статей международной научной конференции (Омск, Март 2023). Crossref, 2023. http://dx.doi.org/10.37539/230310.2023.78.67.003.

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Представлен обзор данных о возможном влиянии фармакогенетических маркеров на эффективность и безопасность этиотропной терапии COVID-19. Рассматриваются клинические исследования ремдесивира, фавипиравира, молнупиравира. Описываются потенциальные фармакогенетические маркеры цитокинов, влияющие на гены семейства цитохромов P450. A review of the data on the possible influence of pharmacogenetic markers on the efficacy and safety of COVID-19 etiotropic therapy is presented. Clinical trials of remdesivir, favipiravir, and molnupiravir are reviewed. Potential pharmacogenetic markers of cytokines affe
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Wu, Huan, Yun-Qiang Di, Chun-Hou Zheng, and Junfeng Xia. "Prediction of cytochrome P450 inhibition using ensemble of extreme learning machine." In 2013 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2013. http://dx.doi.org/10.1109/bibm.2013.6732515.

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Casper, Jessica A., Diane R. Bielenberg, Jennifer Cheng, et al. "Abstract 3952: Cytochrome P450-derived eicosanoids regulate pancreatic cancer and metastasis." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3952.

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Abraham, JE, M. Maranian, K. Driver, et al. "Cytochrome P450 2D6 variants and their association with breast cancer survival." In CTRC-AACR San Antonio Breast Cancer Symposium: 2008 Abstracts. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-3089.

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Hirakawa, Hidehiko, and Teruyuki Nagamune. "Nanoarchitechture of cytochrome P450 system using a ring-shaped protein complex." In 2010 IEEE 10th Conference on Nanotechnology (IEEE-NANO). IEEE, 2010. http://dx.doi.org/10.1109/nano.2010.5698070.

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Reports on the topic "Cytochrome P450"

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Ahsan, Habibul. Cytochrome P450-17alpha Polymorphism and Risk of Breast Cancer. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada390825.

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Arnold, Frances H. Structural and Kinetic Studies of Novel Cytochrome P450 Small-Alkane Hydroxylases. Office of Scientific and Technical Information (OSTI), 2012. http://dx.doi.org/10.2172/1035499.

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Loper, J. C. Third international symposium: Cytochrome P450 biodiversity. Final report, January 1, 1995--December 31, 1995. Office of Scientific and Technical Information (OSTI), 1997. http://dx.doi.org/10.2172/508155.

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Sherr, David H. The Aryl Hydrocarbon (Dioxin) Receptor/Transcription Factor and Cytochrome P450 1B1 as Targets for Breast Cancer Immunotherapy. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada413137.

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Alworth, William L., and David A. Mullin. Use of Genetic Engineering to Produce a Mutated Cytochrome P450 Enzyme Capable of Both Oxidizing and Reductively Dechlorinating Hazardous Organic Chemicals. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada391816.

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Lawanprasert, Somsong, Chaiyo Chaichantipyuth, Supatra Srichairat, Nuansri Niwattisaiwong, and Laddawal Phivthong-ngam. Subchronic exposure of Pueraria mirifica in normal - and high cholesterol diet fed rats : influence on hepatic cytochrome P450, lipid profile and toxicity. Chulalongkorn University, 2004. https://doi.org/10.58837/chula.res.2004.28.

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Pueraria mirifica airy shaw and suvatabandhu, know locally as Kwao Keur, is a plant in family Leguminosae. In this study, effects of P.mirifica on hepatic cytochrome P450 (CYP), serum lipid profile and subchronic toxicity were investigated in male Wistar rats. Rats were randomly divided into four treatment groups as following: normal diet-fed group; normal diet-fed supplemented with P.mirifica group; high cholesterol diet-fed group; high cholesterol diet-fed supplemented with P.mirifica group. Each group comprised 10 rats. P.mirifica was administered orally at a dosage of 100 mg/kg/day for 90
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Amir, Rachel, David J. Oliver, Gad Galili, and Jacline V. Shanks. The Role of Cysteine Partitioning into Glutathione and Methionine Synthesis During Normal and Stress Conditions. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699850.bard.

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The objective of this research is to study the nature of the competition for cysteine (Cys), the first organic sulfur-containing compound, between its two main metabolites, glutathione (GSH) and methionine (Met). GSH plays a central role in protecting plants during various stresses, while Met, an essential amino acid, regulates essential processes and metabolites in plant cells through its metabolite S-adenosyl-Met. Our results, which are based on flux analysis and measurements of Met- metabolites, show that the flux towards Met synthesis is high during non-stress conditions, however the flux
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Meidan, Rina, and Robert Milvae. Regulation of Bovine Corpus Luteum Function. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604935.bard.

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Abstract:
The main goal of this research plan was to elucidate regulatory mechanisms controlling the development, function of the bovine corpus luteum (CL). The CL contains two different sterodigenic cell types and therefore it was necessary to obtain pure cell population. A system was developed in which granulosa and theca interna cells, isolated from a preovulatory follicle, acquired characteristics typical of large (LL) and small (SL) luteal cells, respectively, as judged by several biochemical and morphological criteria. Experiments were conducted to determine the effects of granulosa cells removal
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