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Journal articles on the topic 'Cytofluorimetrie'

Author: Grafiati
Published: 4 June 2021
Last updated: 31 January 2023

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1

Kit, O. I., N. K. Guskova, O. N. Selyutina, V. V. Dmitrieva, I. A. Novikova, I. S. Torpujyan, and V. R. Zakharchenko. "FEATURES OF A DIFFERENTIAL DIAGNOSIS OF PEDIATRIC ACUTE MONOCYTIC LEUKEMIA (AML-M5a) ON THE EXAMPLE OF A CLINICAL CASE." South Russian Journal of Cancer 1, no. 1 (March 7, 2020): 76–83. http://dx.doi.org/10.37748/2687-0533-2020-1-1-7.

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The aim of this work was to assess the significance of investigating clinical and laboratory parameters for diagnosing acute monocytic leukemia in children on the basis of a clinical case. The article demonstrates specific features of differentiating AML M5a from other forms of acute myeloid leukemia. According to the results of hematological, morphological and cytofluorimetric studies of blood and bone marrow samples, the diagnosis of acute myeloid leukemia was established. The morphological and phenotypic characteristics of blast cells hampered the diagnosis of an AML form. However, a comprehensive analysis of the expressed CD antigens allowed acute monocytic leukemia to be identified, which diagnosis was subsequently confirmed by a cytochemical study. Thus, the clinical diagnosis was established over a short period of time. This was of importance given the rising severity of the patient’s condition requiring immediate treatment, the initial hyperleukocytosis and the development of life-threatening complications associated with leukostasis in the lungs and the central nervous system. In the presented case, the clinical manifestations of the underlying disease and the results of flow cytofluorimetry were determining factors in initiating timely specific therapy.
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2

Gesi, Marco, Daniela Galli, Prisco Mirandola, Cecilia Carubbi, Elena Masselli, Marco Vitale, and Giuliana Gobbi. "Cytofluorimetric Platelet Analysis." Seminars in Thrombosis and Hemostasis 40, no. 01 (December 31, 2013): 088–98. http://dx.doi.org/10.1055/s-0033-1363472.

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3

De Figueroa, Jose Manuel Tierno, Francesco Buonocore, Massimo Mazzini, and Giuseppe Scapigliati. "Cytofluorimetric analysis ofBacillus rossiushaemocytes (Phasmatodea, Bacillidae)." Italian Journal of Zoology 68, no. 1 (January 2001): 9–14. http://dx.doi.org/10.1080/11250000109356377.

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4

Brickman, M. J., J. M. Cook, and A. E. Balber. "Low temperature reversibly inhibits transport from tubular endosomes to a perinuclear, acidic compartment in African trypanosomes." Journal of Cell Science 108, no. 11 (November 1, 1995): 3611–21. http://dx.doi.org/10.1242/jcs.108.11.3611.

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We have used electron microscopy and flow cytofluorimetry to study endocytosis and intracellular transport of fluid phase bovine serum albumen gold complexes and membrane bound concanavalin A through endosomal compartments of bloodstream forms of Trypanosoma brucei rhodesiense. Both markers were rapidly endocytosed from the flagellar pocket. Within 20 minutes at 37 degrees C the markers reached a large, vesicular, perinuclear compartment that stained heavily with the CB1 monoclonal antibody. Neither marker left the flagellar pocket and entered cells at 4 degrees C. When cells were incubated at 12 degrees C, both markers entered the cell and were transported to collecting tubules, a tubular endosomal compartment that receives endocytosed material from coated endocytic vesicles. However, no material was transported from collecting tubules to the late, perinuclear compartment at 12 degrees C. The morphology of collecting tubule membranes was specifically altered at 12 degrees C; tubules became shorter and were arrayed near the flagellar pocket. The morphological alteration and the block in transport of endocytic markers to the perinuclear compartment seen at 12 degrees C were reversed 10 minutes after cells were returned to 37 degrees C. We also used flow cytofluorimetric measurements of pH dependent fluorescence quenching to measure the pH of the terminal endocytic compartment. Fluoresceinated lectins accumulated in a terminal compartment with a pH of 6.0-6.1, a value considerably higher than that of mammalian lysosomes. Fluorescence from fluoresceinated lectins in this terminal endocytic compartment was dequenched when bloodstream forms were incubated in the presence of chloroquine.
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5

Testa, Antonia Carla, Francesco Pomini, Andrea Fattorossi, Alessandra Battaglia, Gabriella Ferrandina, Donata Mansueto, Carmen Mastromarino, Giovanni Scambia, and Alessandro Caruso. "Doppler Velocimetry and Cytofluorimetric Analysis inUterine Myomas." Gynecologic and Obstetric Investigation 56, no. 3 (2003): 139–42. http://dx.doi.org/10.1159/000073772.

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6

Dahlström, A., T. Kling-Petersen, S. Bööj, K. Lundmark, and P. A. Larsson. "Quantification of axonally transported material using cytofluorimetric scanning." Journal of Microscopy 155, no. 1 (July 1989): 61–80. http://dx.doi.org/10.1111/j.1365-2818.1989.tb04298.x.

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7

Sazanova, E. V., T. P. Shmelkova, A. L. Kravtsov, T. A. Malyukova, and Yu A. Popov. "FLOW-CYTOFLUORIMETRIC ANALYSIS OF CYTOTOXICITY OF YERSINIA PESTIS STRAINS." Journal of microbiology epidemiology immunobiology, no. 6 (December 28, 2017): 3–9. http://dx.doi.org/10.36233/0372-9311-2017-6-3-9.

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8

Braidotti, Stefania, Raffaella Franca, Marilena Granzotto, Elisa Piscianz, Alberto Tommasini, Marco Rabusin, Gabriele Stocco, and Giuliana Decorti. "Cytofluorimetric assay to investigate variability in blinatumomab in vitro response." Frontiers in Bioscience-Landmark 27, no. 2 (January 24, 2022): 039. http://dx.doi.org/10.31083/j.fbl2702039.

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9

Iannelli, D. "Cytofluorimetric Method for the Detection of the Cucumber Mosaic Virus." Phytopathology 86, no. 9 (1996): 959. http://dx.doi.org/10.1094/phyto-86-959.

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10

Khochbin, Saadi, Agnès Chabanas, Philippe Albert, and Jean-Jacques Lawrence. "Flow cytofluorimetric determination of protein distribution throughout the cell cycle." Cytometry 10, no. 4 (July 1989): 484–89. http://dx.doi.org/10.1002/cyto.990100418.

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11

Oliani, C., D. Barana, A. Cazzadori, E. Zanolin, A. Santo, F. Pasini, M. Padovani, G. Mazzini, and G. L. Cetto. "Cytofluorimetric evaluation of DNA ploidy in lung cancer: A bronchoscopic study." International Journal of Biological Markers 20, no. 2 (2005): 87–92. http://dx.doi.org/10.5301/jbm.2008.3954.

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12

Lauria, Francesco, Gian Paolo Bagnara, Damiano Rondelli, Donatella Raspadori, Pierluigi Strippoli, Laura Bonsi, Maria Alessandra Ventura, et al. "Cytofluorimetric and Functional Analysis of C-Kit Receptor in Acute Leukemia." Leukemia & Lymphoma 18, no. 5-6 (January 1995): 451–55. http://dx.doi.org/10.3109/10428199509059644.

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13

Oliani, C., D. Barana, A. Cazzadori, E. Zanolin, A. Santo, F. Pasini, M. Padovani, G. Mazzini, and G. L. Cetto. "Cytofluorimetric Evaluation of DNA Ploidy in Lung Cancer: A Bronchoscopic Study." International Journal of Biological Markers 20, no. 2 (April 2005): 87–92. http://dx.doi.org/10.1177/172460080502000202.

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The study of the biological characteristics of lung cancer is gaining more and more interest both because of their potential role as prognostic indicators and for therapeutic reasons. The DNA content estimated by flow cytometry in surgical samples of non-small cell lung cancer (NSCLC) has already been demonstrated to be correlated with survival in these patients. From July 1990 to February 1992 we analyzed the DNA distribution of bronchoscopic biopsies from 88 patients with lung cancer (18 small cell lung cancer, SCLC, and 68 NSCLC, two unspecified histology). Twenty-eight tumors (34.6%) had a diploid DNA distribution, while 53 were aneuploid (65.4%). A correlation was found between DNA ploidy and survival. Evaluation of the DNA content in bronchoscopic samples in a large series of patients could determine the role of this analysis prior to surgery in NSCLC and its value as a marker with respect to prognosis and response to therapy in SCLC.
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14

Miranda, A., J. de León, L. Roque-Navarro, and L. E. Fernández. "Cytofluorimetric evaluation of N-glycolylated GM3 ganglioside expression on murine leukocytes." Immunology Letters 137, no. 1-2 (June 2011): 38–45. http://dx.doi.org/10.1016/j.imlet.2011.02.001.

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15

Pavlov, O. V., S. V. Chepanov, A. V. Selutin, M. S. Zainulina, D. R. Eremeeva, and S. A. Selkov. "Flow cytofluorimetric detection and immunophenotyping of platelet-monocyte complexes in peripheral blood." Medical Immunology (Russia) 23, no. 2 (May 3, 2021): 401–10. http://dx.doi.org/10.15789/1563-0625-fcd-2124.

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Activated platelets aggregate with monocytes by binding membrane bound molecules. Platelet-monocyte interaction is considered to underlie pathophysiological mechanisms bridging thrombosis and inflammation. Detection and analysis of platelet-monocyte complexes (PMC) provide means for revealing their physiological and pathogenetic roles and are instrumental in the diagnostics of various pathological conditions including obstetric complications. The aim of the study was to develop the method of quantitative determination of peripheral blood PMC, that preserve phenotypic features of platelets and monocytes, and to reveal their changes by ex vivo analysis. The suggested procedure includes immediate fixation of blood sample, immunocytochemical staining with fluorochrome-conjugated specific antibodies against markers of activation and differentiation followed by lysis of erythrocytes, and flow cytometric analysis. Fourteen samples of peripheral blood from patients with history of pregnancy complication were obtained in first trimester of ongoing pregnancy and analyzed. It was demonstrated that quantitative and qualitative in vivo characteristics of PMC remained unchanged in fixed samples, whereas the number of PMC and expression levels of the markers of platelet and monocyte activation dramatically increased in the unfixed blood. The set of monoclonal antibodies and gating strategies, used in this study, ensure phenotyping and evaluation of percentage/absolute count of PMC in the total monocyte population (CD45+CD14+) and in the subpopulations of classical (CD14+CD16-), intermediate (CD14+CD16+), and non-classical (CD14lowCD16+) monocytes. This approach provides insight into the participation of different monocyte subsets in the formation of PMC and their roles in physiological and pathophysiological processes. In some samples, elevated PMC proportion was observed, accompanied by significant increase in the expression of platelet activation marker CD62P and decrease in the expression of its monocytic ligand CD162. These changes suggested altered activation of PMC and their participation in the pathophysiological mechanisms of some pregnancy complications. Immunophenotyping of PMC affords an opportunity to characterize their proinflammatory, procoagulant and adhesive properties; these results can be used for research and diagnostics. In particular, the method is suitable for detection and phenotyping of PMC in pregnancy complications and other pathological conditions associated with the disorders of hemostasis and thrombosis.
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16

Bernard-Beaubois, K., C. Hecquet, P. Rat, and M. Adolphe. "Quinolones tenotoxicity evaluation using direct microplate cytofluorimetric assays in alive tenocytes. (MiFAAC)." Toxicology Letters 88 (October 1996): 41. http://dx.doi.org/10.1016/s0378-4274(96)80147-0.

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17

Larsson, Pär-Anders, Serney Bööj, Kerstin Lundmark, Menek Goldstein, and Annica Dahlström. "Reserpine-induced effects in the adrenergic neuron as studied with cytofluorimetric scanning." Brain Research Bulletin 16, no. 1 (January 1986): 63–74. http://dx.doi.org/10.1016/0361-9230(86)90013-4.

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18

Giacomini, P., A. Settini, R. Fraioli, A. Martayan, N. Vitale, G. Ciccarelli, I. Venturo, et al. "Cytofluorimetric analysis of HLA class I expression in non-linphoid neoplastic cells." Human Immunology 47, no. 1-2 (April 1996): 25. http://dx.doi.org/10.1016/0198-8859(96)84813-0.

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19

Riccardi, A., C. M. Montecucco, M. Danova, M. Girino, G. Ucci, P. Giordano, and G. Mazzini. "L3 acute leukemia: Cytofluorimetric abnormalities of DNA content at presentation and relapse." Leukemia Research 10, no. 1 (January 1986): 99. http://dx.doi.org/10.1016/0145-2126(86)90147-5.

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20

Rossi, C. "General software for the analysis of cytofluorimetric data by E-M methods." Computers & Mathematics with Applications 14, no. 9-12 (1987): 771–82. http://dx.doi.org/10.1016/0898-1221(87)90226-4.

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21

Levi, Marisa, Davide Colombo, Sergio Sgorbati, Donato Chiatante, and Elio Sparvoli. "Effect of Benzyladenine on Cell Cycle in Intact Roots. A Cytofluorimetric Approach." Journal of Plant Physiology 131, no. 5 (December 1987): 385–91. http://dx.doi.org/10.1016/s0176-1617(87)80281-x.

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22

Kvedariene, V., S. Kamey, Y. Ryckwaert, M. Rongier, J. Bousquet, P. Demoly, and B. Arnoux. "Diagnosis of neuromuscular blocking agent hypersensitivity reactions using cytofluorimetric analysis of basophils." Allergy 61, no. 3 (March 2006): 311–15. http://dx.doi.org/10.1111/j.1398-9995.2006.00978.x.

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23

Sgorbati, S., E. Sparvoli, M. Levi, D. Chiatante, and P. Giordano. "Bivariate cytofluorimetric analysis of DNA and nuclear protein content in plant tissue." Protoplasma 144, no. 2-3 (June 1988): 180–84. http://dx.doi.org/10.1007/bf01637251.

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24

Mbida, A. D., B. Pozzetto, O. Sabido, Y. Akono, F. Grattard, M. Habib, and O. G. Gaudin. "Competition binding studies with biotinylated echovirus 11 in cytofluorimetry analysis." Journal of Virological Methods 35, no. 2 (November 1991): 169–76. http://dx.doi.org/10.1016/0166-0934(91)90132-j.

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25

Sabido, Odile, D. Mbida André, Bruno Pozetto, Odette Gaudin, and François Berthoux. "COMPETITION BINDING STUDIES WITH BIOTINYLATED ECHOVIRUS 11 IN CYTOFLUORIMETRY ANALYSIS." Biology of the Cell 73, no. 2-3 (January 1991): 47a. http://dx.doi.org/10.1016/0248-4900(91)90252-i.

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26

Jacob, Marie-Christine, Mireille Favre, and Jean-Claude Bensa. "EVALUATION OF CD4 CD45RA LYMPHOCYTES IN MALIGNANT LYMPHOMA BY CYTOFLUORIMETRY." Biology of the Cell 73, no. 2-3 (January 1991): 50a. http://dx.doi.org/10.1016/0248-4900(91)90263-m.

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27

Dimitropoulos, K., J. M. Rolland, and R. C. Nairn. "Flow cytofluorimetry of fluorescein fluorescence polarization to assay lymphocyte activation." Biochemical and Biophysical Research Communications 136, no. 3 (May 1986): 1021–29. http://dx.doi.org/10.1016/0006-291x(86)90435-3.

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28

Saggia, C., A. Santagostino, G. Forti, G. Biaggi, G. Angeli, M. Dacorsi, D. Cerrato, G. Loi, G. Porcile, and O. Alabiso. "Prospective study on prognostic significance of DNA ploidy and Ki-67 expression in colorectal cancer." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 21184. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.21184.

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21184 Background: The prognostic value of flow cytometry DNA ploidy and Ki-67 expression in colorectal carcinomas has not been defined yet. The study tries to correlate tumoral DNA ploidy and proliferative activity (Ki-67) with therapy response, Overall Survival (OS), Disesase Specific Survival (DSS) and Disease Free Survival (DFS). Methods: From 01/09/02 to 30/06/05, 3 samples of cancer tissue and 1 sample of normal mucosa has been collected from all operating pieces of colorectal cancer. These samples were frosen and later disgregated, treated and coloured with Propidium Iodide. DNA ploidy was evaluated by FACSCalibur cytofluorimetry. Normal mucosa tissue was our internal control. Ki-67 was evaluated by immuno-histochemistry in all tumoral samples. All the patients were cured with chemo- and/or radiotherapy in our divisions. Results: 67 patients (M/F 35/32); median age was 70; staging: 19% I, 33% II, 30% III, 18% IV. We found aneuploidy in 65,7% of carcinoma and Ki-67 median expression was 55%. DNA tumoral heterogeneity was present in 27% of patients. DNA aneuploidy correlates with advanced disease stage at diagnosis (p<0,01), with high number of metastatic lymphnodes (p<0,005) and with serological markers positivity (p<0,04). After surgery and chemotherapy 35% of the patients with aneuploid carcinoma and high proliferative activity (Ki-67>55%) do not show evident disease versus 100% of patients with DNA diploidy and lower Ki-67. Tumoral aneuploidy correlates in a significative way with lower OS (48% vs 89% of diploid patients), lower DSS (tumor death happened just in patients with aneuploid DNA in every disease stages), with lower DFS (18% vs 86% of diploid patients). Univariated analysis stated that aneuploidy determinates an Odd Ratio=5,7 to develop disease progression (p=0,033). At the moment Ki-67 expression with 55% cut-off does not seem to correlate with OS, DSS and DFS. Conclusions: Preliminar results (the study is still in progress) seem to suggest that cytofluorimetric DNA-ploidy has a prognostic and predictive significance in colo-rectal carcinomas. Ki-67 expression (immuno-histochemistry) has an uncertain significance. The small number of patients and the short follow-up do not allow us to reach any definitive conclusions, but the study is worth to go on. No significant financial relationships to disclose.
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29

Zerboni, Alessandra, Rossella Bengalli, Luisa Fiandra, Tiziano Catelani, and Paride Mantecca. "Cellular Mechanisms Involved in the Combined Toxic Effects of Diesel Exhaust and Metal Oxide Nanoparticles." Nanomaterials 11, no. 6 (May 29, 2021): 1437. http://dx.doi.org/10.3390/nano11061437.

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Diesel exhaust particles (DEPs) and non-exhaust particles from abrasion are two main representative sources of air pollution to which humans are exposed daily, together with emerging nanomaterials, whose emission is increasing considerably. In the present work, we aimed to investigate whether DEPs, metal oxide nanoparticles (MeO-NPs), and their mixtures could affect alveolar cells. The research was focused on whether NPs induced different types of death in cells, and on their effects on cell motility and migration. Autophagy and cell cycles were investigated via cytofluorimetric analyses, through the quantification of the autophagic biomarker LC3B and PI staining, respectively. Cellular ultrastructures were then observed via TEM. Changes in cell motility and migration were assessed via transwell migration assay, and by the cytofluorimetric analysis of E-cadherin expression. A colony-forming efficiency (CFE) assay was performed in order to investigate the interactions between cells inside the colonies, and to see how these interactions change after exposure to the single particles or their mixtures. The results obtained suggest that NPs can either reduce the toxicity of DEPs (CuO) or enhance it (ZnO), through a mechanism that may involve autophagy as cells’ response to stressors and as a consequence of particles’ cellular uptake. Moreover, NPs can induce modification of E-cadherin expression and, consequentially, of colonies’ phenotypes.
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Santoni, Giorgio, Massimo Nabissi, Consuelo Amantini, Matteo Santoni, Lucia Ricci-Vitiani, Roberto Pallini, Federica Maggi, and Maria Beatrice Morelli. "ERK Phosphorylation Regulates the Aml1/Runx1 Splice Variants and the TRP Channels Expression during the Differentiation of Glioma Stem Cell Lines." Cells 10, no. 8 (August 10, 2021): 2052. http://dx.doi.org/10.3390/cells10082052.

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The identification of cancer stem cells in brain tumors paved the way for new therapeutic approaches. Recently, a role for the transcriptional factor Runx1/Aml1 and the downstream ion channel genes in brain cancer development and progression has been suggested. This study aimed to explore the expression and the role of Runx1/Aml1, its Aml1b and Aml1c splice variants and the downstream TRPA1 and TRPV1 ion channels in undifferentiated and day-14 differentiated neural stem cells (NSCs and D-NSCs) and glioblastoma stem cells (GSCs and D-GSCs) lines with different proneural (PN) or mesenchymal (MES) phenotype. Gene and protein expression were evaluated by qRT-PCR, cytofluorimetric, western blot and confocal microscopy analyses. Moreover, by western blot, we observed that ERK phosphorylation enhances the Aml1b and Aml1c protein expression during glioma differentiation. Furthermore, the agonists of TRPA1 and TRPV1 channels stimulated apoptosis/necrosis in GSCs and D-GSCs as evaluated by Annexin V and PI staining and cytofluorimetric analysis. Finally, by qRT-PCR, the modulation of Wnt/β catenin, FGF, and TGFβ/SMAD signaling pathways in PN- and MES-GSCs was reported. Overall, our results provide new evidence regarding Runx1/Aml1 isoform overexpression and modulation in TRP channel expression during gliomagenesis, thus offering new directions for glioblastoma therapy.
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31

Cuoghi, Barbara, and Lucrezia Mola. "Morphological, Cytochemical, and Cytofluorimetric Features of Supramedullary Neurons of the Fish Solea ocellata." Biological Bulletin 212, no. 1 (February 2007): 1–5. http://dx.doi.org/10.2307/25066574.

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32

Camassei, Francesca Diomedi, Cristiano Ferlini, Alessandro Jenkner, Cesare Bosman, Roberto Biselli, Alberto Donfrancesco, and Renata Boldrini. "NEPHROBLASTOMA. DNA CHARACTERISTICS AND THEIR MODIFICATIONS INDUCED BY PRENEPHRECTOMY CHEMOTHERAPY: A CYTOFLUORIMETRIC STUDY." Pediatric Pathology & Molecular Medicine 21, no. 1 (January 2002): 15–23. http://dx.doi.org/10.1080/pdp.21.1.15.23.

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Camassei, Francesca Diomedi, Cristiano Ferlini, Alessandro Jenkner, Cesare Bosman, Roberto Biselli, Alberto Donfrancesco, and Renata Boldrini. "NEPHROBLASTOMA. DNA CHARACTERISTICS AND THEIR MODIFICATIONS INDUCED BY PRENEPHRECTOMY CHEMOTHERAPY: A CYTOFLUORIMETRIC STUDY." Pediatric Pathology & Molecular Medicine 21, no. 1 (January 1, 2002): 15–23. http://dx.doi.org/10.1080/15227950252774129.

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Dicarlo, Manuela, Gabriella Teti, Iolanda Iezzi, Giorgia Cerqueni, Sandra Manzotti, Mirella Falconi, and Monica Mattioli-Belmonte. "Detecting senescent fate in mesenchymal stem cells: a combined cytofluorimetric and ultrastructural approach." Biogerontology 19, no. 5 (August 12, 2018): 401–14. http://dx.doi.org/10.1007/s10522-018-9766-4.

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35

Winstanley, F. P. "Detection of TNF alpha receptors on ovine leucocytes by flow cytofluorimetry." Research in Veterinary Science 52, no. 1 (January 1992): 129–31. http://dx.doi.org/10.1016/0034-5288(92)90074-c.

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36

Monica, B., and R. Minari. "Indici di proliferazione (Ki 67, TLI) ed espressione della p53. Esperienza personale: Proliferation indices (Ki 67, TLI) and p53 expression. Personal experience." Urologia Journal 62, no. 2 (April 1995): 234–39. http://dx.doi.org/10.1177/039156039506200210.

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The Authors report their experience on the study of urothelial bladder cancer by determining the overexpression of p53, DNA-content by flow-cytometry, Ki 67, TLI and cytofluorimetric S-phase in 81 patients. Ki 67, DNA content and p53 overexpression are statistically related with histologic grade, unlike TLI and S-phase. TLI, overexpression of p53 and Ki 67 are statistically related with stage, whereas ploidy and S-phase do not show any relation with stage. The Authors discuss these findings.
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Palomba, Emanuela, Valentina Tirelli, Elisabetta de Alteriis, Palma Parascandola, Carmine Landi, Stefano Mazzoleni, and Massimo Sanchez. "A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor." PLOS ONE 16, no. 6 (June 10, 2021): e0248382. http://dx.doi.org/10.1371/journal.pone.0248382.

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The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
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Minervini, F., R. Guastamacchia, F. Pizzi, ME Dell’Aquila, and VL Barile. "Assessment of Different Functional Parameters of Frozen-Thawed Buffalo Spermatozoa by Using Cytofluorimetric Determinations." Reproduction in Domestic Animals 48, no. 2 (July 27, 2012): 317–24. http://dx.doi.org/10.1111/j.1439-0531.2012.02152.x.

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39

Danova, M., A. Riccardi, G. Ucci, R. Luoni, M. Giordano, and G. Mazzini. "Ras oncogene expression and DNA content in plasma cell dyscrasias: a flow cytofluorimetric study." British Journal of Cancer 62, no. 5 (November 1990): 781–85. http://dx.doi.org/10.1038/bjc.1990.379.

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40

Niccolai, Elena, Danilo Squatrito, Giacomo Emmi, Elena Silvestri, Lorenzo Emmi, Lucia Ciucciarelli, Federica Ricci, Daniele Manganaro, Amedeo Amedei, and Domenico Prisco. "A new cytofluorimetric approach to evaluate the circulating microparticles in subjects with antiphospholipid antibodies." Thrombosis Research 136, no. 6 (December 2015): 1252–58. http://dx.doi.org/10.1016/j.thromres.2015.10.018.

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Pâris-Köhler, Aurélie, Pascal Demoly, Laurence Persi, Bernard Lebel, Jean Bousquet, and Bernard Arnoux. "In vitro diagnosis of cypress pollen allergy by using cytofluorimetric analysis of basophils (Basotest)." Journal of Allergy and Clinical Immunology 105, no. 2 (February 2000): 339–45. http://dx.doi.org/10.1016/s0091-6749(00)90085-x.

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42

Hess, Ralf D., Markus Kuther, Christel Haessler, Susanne Paetzold, Dietmar G. Braun, and Gerhard Brandner. "Quantitative cytofluorimetric determination of cell membrane-associated large tumor antigen on SV40-transformed cells." Cytometry 20, no. 1 (May 1, 1995): 81–85. http://dx.doi.org/10.1002/cyto.990200112.

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43

Trubiani, O., R. Di Primio, T. Traini, J. Pizzicannella, A. Scarano, A. Piattelli, and S. Caputi. "Morphological and Cytofluorimetric Analysis of Adult Mesenchymal Stem Cells Expanded Ex Vivo from Periodontal Ligament." International Journal of Immunopathology and Pharmacology 18, no. 2 (April 2005): 213–21. http://dx.doi.org/10.1177/039463200501800204.

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Many adult tissues contain a population of stem cells that have the ability of regeneration after trauma, disease or aging. Recently, there has been great interest in mesenchymal stem cells and their roles in maintaining physiological structure tissues and their studies have been considered very important and intriguing after having shown that this cell population can be expanded ex vivo to regenerate tissues not only of the mesenchymal lineage, such as intervertebral disc cartilage, bone, tooth-associated tissue, cardiomyocytes, but also to differentiate into cells derived from other embryonic layers, including neurons. Currently, different efforts have been focused on the identification of odontogenic progenitors from oral tissues. In this study we isolated and characterized a population of homogeneous human mesenchymal stem cells proliferating in culture with an attached well-spread morphology derived from periodontal ligament, tissue of ectomesenchymal origin, with the ability to form a specialized joint between alveolar bone and tooth. The adherent cells were harvested and expanded ex vivo under specific conditions and analysed by FACScan flow cytometer and morphological analysis was carried out by light, scanning and transmission electron microscopy. Our results displayed highly evident cells with a fibroblast like morphology and a secretory apparatus, probably indicating, that the enhanced function of the secretory apparatus of the mesenchymal stem cells may be associated with the secretion of molecules that are required to survive and proliferate. Moreover, the presence in periodontal ligament of CD90, CD29, CD44, CD166, CD 105, CD13 positive cells, antigens that are also identified as stromal precursors of the bone marrow, indicate that the periodontal ligament may turn out to be a new efficient source of the cells with intrinsic capacity to self-renewal, high ability to proliferate and differentiate, that can be utilized for a new approach to regenerative medicine and tissue engineering.
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GALLI, MARIA GRAZIA, ROBERTA BALZARETTI, and SERGIO SGORBATII. "Autoradiographic and Cytofluorimetric Analysis of DNA Synthesis in Endosperm and Cotyledons of Germinating Castor Bean." Journal of Experimental Botany 37, no. 11 (1986): 1716–24. http://dx.doi.org/10.1093/jxb/37.11.1716.

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45

Lina, Gerard, Grégoire Cozon, Josette Ferrandiz, Timothy Greenland, François Vandenesch, and Jerome Etienne. "Detection of Staphylococcal Superantigenic Toxins by a CD69-Specific Cytofluorimetric Assay Measuring T-Cell Activation." Journal of Clinical Microbiology 36, no. 4 (1998): 1042–45. http://dx.doi.org/10.1128/jcm.36.4.1042-1045.1998.

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The presence of staphylococcal superantigenic toxins in the supernatants of liquid cultures was detected by an easy and rapid method assessing the activation of T lymphocytes by cytofluorimetric measurement of CD69 expression. Staphylococcus aureus cells were grown in Eagle’s minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. aureus strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, and exfoliative toxins A and B, induced CD69 expression in a significantly higher number of T cells than a cutoff of 2%. This CD69 assay might be used for initial detection of superantigens from S. aureus strains isolated in the context of staphylococcal toxemia or related chronic human diseases such as atopic dermatitis or Kawasaki syndrome.
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MANCA, E., V. ARANGINO, S. LOMBARDINI, A. GHIANI, D. MUSU, B. AMBROSINI, S. R. GIACCO, and G. S. GIACCO. "Triple-Labeling Cytofluorimetric Quantitative and Qualitative Evaluation of Phagocytic Activity of Monocytes and Polymorphonuclear Cells." Annals of the New York Academy of Sciences 832, no. 1 Phagocytes (December 1997): 21–28. http://dx.doi.org/10.1111/j.1749-6632.1997.tb46234.x.

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47

Ellis, J. A., W. C. Davis, E. L. Belden, and D. L. Pratt. "Flow Cytofluorimetric Analysis of Lymphocyte Subset Alterations in Cattle Infected with Bovine Viral Diarrhea Virus." Veterinary Pathology 25, no. 3 (May 1988): 231–36. http://dx.doi.org/10.1177/030098588802500308.

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Clinically normal, bovine viral diarrhea virus (BVDV)-seronegative, 7 to 9-month-old steers were inoculated intranasally with NY-1. a noncytopathic strain of BVDV, or exposed intramuscularly to killed BVDV. Indirect immunofluorescence staining with monoclonal antibodies specific for bovine leukocyte subsets followed by flow cytometric analysis was used to monitor subsequent hematologic alterations. Infection with BVDV resulted in a transient leukopenia which was characterized by decreases in the absolute numbers of circulating T lymphocytes, including BoT4+ (“helper”) and BoT8+ (“cytotoxic/suppressor”) subsets, B lymphocytes, and neutrophils. There was no significant variation in numbers of non-T, non-B (“null”) lymphocytes or monocytes. Exposure to inactivated BVDV in a combination vaccine did not cause significant alteration in the circulating numbers of any major leukocyte subset; however, significant variation was seen in the BoT4/BoT8 ratios and in the numbers of cells expressing major histocompatibility complex (MHC) class II antigens.
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48

Goossens, P. L., H. Jouin, and G. Milon. "Dynamics of lymphocytes and inflammatory cells recruited in liver during murine listeriosis. A cytofluorimetric study." Journal of Immunology 147, no. 10 (November 15, 1991): 3514–20. http://dx.doi.org/10.4049/jimmunol.147.10.3514.

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Abstract During primary infection of mice by Listeria monocytogenes, bacterial elimination is dependent on the recruitment of myelomonocytic cells in the infectious foci and the activation of their bactericidal mechanisms through cytokines secreted by Listeria-specific T lymphocytes. The immune events occurring in the liver, one of the main infected organs, have not yet been studied in detail. In the present quantitative study, we describe the dynamics of recruitment of cells belonging to the lymphoid or myelomonocytic lineages in the liver. The different cell populations mobilized into the liver were isolated each day during the course of a sublethal L. monocytogenes infection and their phenotype was characterized by flow cytometry. Three distinct phases of recruitment were observed. 1) During the first day of infection, 17 x 10(6) lymphomyeloid cells were recruited in the liver with a predominance of myelomonocytic cells; 51% of the incoming cells were M1/70+; the NK cell population (detected by the 4D11 antibody) also increased transiently at this period. 2) From day 3 to 5, a high number of myelomonocytic cells infiltrated the liver (13 x 10(6) M1/70+ cells); most of these cells were macrophages (as detected with the macrophage-restricted antibody FA/11 or observed after May-Grünwald Giemsa staining); the antigranulocytic antibodies 7/4 and RB6.8C5 were found to label these mononuclear phagocytes at this period of infection. A subpopulation of Thy-1+ cells (16%) was found to be labeled by the RB6.8C5 antibody in normal liver and, at day 5 and 6, all Thy-1+ cells also bound the RB6.8C5 mAb.3) From day 5 onward, two waves of phenotypically distinct T lymphocytes were observed; the number of CD8+ T lymphocytes (15 x 10(6) cells) increased first at day 5 and peaked on day 7; CD4+ T lymphocytes (6.2 x 10(6) cells) were then recruited with a 2-day delay (on day 7) in the liver.
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Pistillo, M. P., P. L. Tazzari, O. Mazzoleni, A. Urlacher, M. Falco, M. Vitale, R. W. Karr, and G. B. Ferrara. "ANALYSIS OF HLA SPECIFICITY OF HUMAN MONOCLONAL ANTIBODIES BY CYTOFLUORIMETRY AND CELL ELISA." European Journal of Immunogenetics 18, no. 5-6 (October 1991): 345–53. http://dx.doi.org/10.1111/j.1744-313x.1991.tb00034.x.

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50

Rat, P., K. Bemard-Beaubois, N. Moniot-Ville, Vincen du Laurier, M. Adolphe, and J. M. Warnet. "New quinolones cytotoxicity assessment on living tenocytes in microplates using cold light cytofluorimetry." Toxicology Letters 95 (July 1998): 198–99. http://dx.doi.org/10.1016/s0378-4274(98)80791-1.

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