Log in

Relevant bibliographies by topics / D-Allulose / Journal articles

To see the other types of publications on this topic, follow the link: D-Allulose.

Journal articles on the topic 'D-Allulose'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'D-Allulose.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Lee, Dayoun, Youngji Han, Eun-Young Kwon, and Myung-Sook Choi. "d-allulose Ameliorates Metabolic Dysfunction in C57BL/KsJ-db/db Mice." Molecules 25, no. 16 (2020): 3656. http://dx.doi.org/10.3390/molecules25163656.

Full text

Abstract:

d-allulose is an uncommon sugar that provides almost no calories when consumed. Its sweetness is 70% that of sucrose. d-allulose is a metabolic regulator of glucose and lipid metabolism. However, few reports concerning its effect on diabetes and related metabolic disturbances in db/db mice are available. In this study, we evaluated d-allulose’s effect on hyperglycemia, hyperinsulinemia, diabetes and inflammatory responses in C57BL/KsJ-db/db mice. Mice were divided into normal diet, erythritol supplemented (5% w/w), and d-allulose supplemented (5% w/w) groups. Blood glucose and plasma glucagon

APA, Harvard, Vancouver, ISO, and other styles

2

Molonia, Maria Sofia, Federica Lina Salamone, Antonio Speciale, Antonella Saija, and Francesco Cimino. "D-Allulose Reduces Hypertrophy and Endoplasmic Reticulum Stress Induced by Palmitic Acid in Murine 3T3-L1 Adipocytes." International Journal of Molecular Sciences 25, no. 7 (2024): 4059. http://dx.doi.org/10.3390/ijms25074059.

Full text

Abstract:

Natural rare sugars are an alternative category of sweeteners with positive physiologic and metabolic effects both in in vitro and animal models. D-allulose is a D-fructose epimer that combines 70% sucrose sweetness with the advantage of an extremely low energy content. However, there are no data about the effect of D-allulose against adipose dysfunction; thus, it remains to be confirmed whether D-allulose is useful in the prevention and in treatment of adipose tissue alterations. With this aim, we evaluated D-allulose’s preventive effects on lipid accumulation in 3T3-L1 murine adipocytes expo

APA, Harvard, Vancouver, ISO, and other styles

3

Xia, Yu, Qianqian Cheng, Wanmeng Mu, et al. "Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes." Foods 10, no. 9 (2021): 2186. http://dx.doi.org/10.3390/foods10092186.

Full text

Abstract:

d-allulose has a significant application value as a sugar substitute, not only as a food ingredient and dietary supplement, but also with various physiological functions, such as improving insulin resistance, anti-obesity, and regulating glucolipid metabolism. Over the decades, the physiological functions of d-allulose and the corresponding mechanisms have been studied deeply, and this product has been applied to various foods to enhance food quality and prolong shelf life. In recent years, biotransformation technologies for the production of d-allulose using enzymatic approaches have gained m

APA, Harvard, Vancouver, ISO, and other styles

4

Rakhat, Yermek, Kentaro Kaneko, Lei Wang, et al. "d-Allulose Inhibits Ghrelin-Responsive, Glucose-Sensitive and Neuropeptide Y Neurons in the Arcuate Nucleus and Central Injection Suppresses Appetite-Associated Food Intake in Mice." Nutrients 14, no. 15 (2022): 3117. http://dx.doi.org/10.3390/nu14153117.

Full text

Abstract:

d-allulose, a rare sugar, has sweetness with few calories. d-allulose regulates feeding and glycemia, and ameliorates hyperphagia, obesity and diabetes. All these functions involve the central nervous system. However, central mechanisms underlying these effects of d-allulose remain unknown. We recently reported that d-allulose activates the anorexigenic neurons in the hypothalamic arcuate nucleus (ARC), the neurons that respond to glucagon-like peptide-1 and that express proopiomelanocortin. However, its action on the orexigenic neurons remains unknown. This study investigated the effects of d

APA, Harvard, Vancouver, ISO, and other styles

5

Tan, Jin Hao, Anqi Chen, Jiawu Bi, Yee Hwee Lim, Fong Tian Wong, and Dave Siak-Wei Ow. "The Engineering, Expression, and Immobilization of Epimerases for D-allulose Production." International Journal of Molecular Sciences 24, no. 16 (2023): 12703. http://dx.doi.org/10.3390/ijms241612703.

Full text

Abstract:

The rare sugar D-allulose is a potential replacement for sucrose with a wide range of health benefits. Conventional production involves the employment of the Izumoring strategy, which utilises D-allulose 3-epimerase (DAEase) or D-psicose 3-epimerase (DPEase) to convert D-fructose into D-allulose. Additionally, the process can also utilise D-tagatose 3-epimerase (DTEase). However, the process is not efficient due to the poor thermotolerance of the enzymes and low conversion rates between the sugars. This review describes three newly identified DAEases that possess desirable properties for the i

APA, Harvard, Vancouver, ISO, and other styles

6

Niibo, Misato, Akane Kanasaki, Tetsuo Iida, et al. "d-allulose protects against diabetic nephropathy progression in Otsuka Long-Evans Tokushima Fatty rats with type 2 diabetes." PLOS ONE 17, no. 1 (2022): e0263300. http://dx.doi.org/10.1371/journal.pone.0263300.

Full text

Abstract:

d-allulose is a rare sugar that has been reported to possess anti-hyperglycemic effects. In the present study, we hypothesized that d-allulose is effective in attenuating the progression of diabetic nephropathy in the Otsuka Long-Evans Tokushima Fatty (OLETF) rat model of type 2 diabetes mellitus. Drinking water with or without 3% d-allulose was administered to OLETF rats for 13 weeks. Long-Evans Tokushima Otsuka rats that received drinking water without d-allulose were used as non-diabetic control rats. d-allulose significantly attenuated the increase in blood glucose levels and progressive m

APA, Harvard, Vancouver, ISO, and other styles

7

Shigeru, Suna* and Masaaki Tokuda. "Effect of D-allulose on di-n-butyl phthalate (DBP) toxicity in rats." International Journal of Pharmaceutical Science and Health Care 15, no. 3 (2025): 10–18. https://doi.org/10.5281/zenodo.15535292.

Full text

Abstract:

<strong>Abstract</strong> <strong>Background</strong>: Oral exposure to high concentrations of di-n-butyl phthalate (DBP) causes testicular and hepatotoxicity in rodents. DBP-metabolite, mono-n-butyl phthalate (MBP) stimulates peroxisome proliferator-activated receptors and disrupts carbohydrate and lipid metabolism. The oxidative stress generated may be closely related to these toxicities. <strong>Method</strong>: To determine the effect of D-allulose on DBP-induced testicular and hepatotoxicity, rats were fed a DBP (1% or 2%) diet and D-allulose (2%) in the drinking water. <strong>Result</st

APA, Harvard, Vancouver, ISO, and other styles

8

Shigeru Suna and Masaaki Tokuda. "Effects of D-allulose on di (2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP)-induced toxicity in rats." World Journal of Biological and Pharmaceutical Research 6, no. 1 (2024): 001–7. http://dx.doi.org/10.53346/wjbpr.2024.6.1.0076.

Full text

Abstract:

Background: Oral exposure to high concentrations of DEHP and DBP causes testicular and hepatotoxicity in rodents. Phthalate metabolites such as mono (2-ethylhexyl) phthalate (MEHP) and mono-n-butyl phthalate (MBP) stimulate peroxisome proliferator-activated receptors and disrupts carbohydrate and lipid metabolism. The oxidative stress generated may be closely related to these toxicities. Method: To clarify the effects of the rare sugar D-allulose, a potent free radical scavenger, on testicular and hepatotoxicity induced by DEHP and DBP, rats were fed DEHP or DBP containing diet and D-allulose

APA, Harvard, Vancouver, ISO, and other styles

9

Koike, Teruhiko, Yang Gou, Bingyang Liu, et al. "EFFECT OF D-ALLULOSE ON INSULIN RESISTANCE AND ENDURANCE ABILITY: IS D-ALLULOSE AN EXERCISE MIMETIC?" Innovation in Aging 8, Supplement_1 (2024): 876. https://doi.org/10.1093/geroni/igae098.2833.

Full text

Abstract:

Abstract D-Allulose, a rare sugar and C3-epimer of D-fructose has been proposed as a candidate for dietary restriction mimetics via inhibition of glycolysis. Anti-hyperglycemic and anti-obesity effects have been reported in rodents and human subjects. Here, we report that D-allulose increases the glucose infusion rate in the two-step hyperinsulinemic-euglycemic clamp test in high-fat or high-sucrose diet-fed rat models. Consistently, the insulin-induced phosphorylation of AKT in the skeletal muscle was higher with D-allulose administration with decreased levels of inflammatory cytokine, TNF-α.

APA, Harvard, Vancouver, ISO, and other styles

10

Matsuo, Tatsuhiro, Chihiro Yokoyama, Takako Yamada, et al. "Effect of Simultaneous Intake of Medium-Chain Triglyceride and d-Allulose on Body Fat Accumulation in Rats Fed a High-Fat Diet." CURRENT TOPICS IN NUTRACEUTICAL RESEARCH 21, no. 3 (2023): 242–47. http://dx.doi.org/10.37290/ctnr2641-452x.21:242-247.

Full text

Abstract:

Medium-chain triglycerides, lipids containing three 6−12 carbon medium-chain fatty acids, have antiobesity effects because they do not promote lipogenesis. d-Allulose, a low-calorie epimer of fructose commercially used as a low-calorie sweetener, suppresses hepatic lipogenesis and enhances postprandial fat oxidation. Therefore, we have explored whether a simultaneous intake of medium-chain fatty acids and d-allulose may exhibit a greater reduction in de novo lipogenesis and increase their antiobesity effects. To this end, 32 male Wistar rats were divided into four treatment groups of equal siz

APA, Harvard, Vancouver, ISO, and other styles

11

Sari, Flori R. "The Potential Effect of Honey-derived D-Allulose in Counteracting Hyperglycemia by Time and Dose Dependent Manner in Diabetes Mellitus." Jurnal Kimia Valensi 9, no. 2 (2023): 313–20. http://dx.doi.org/10.15408/jkv.v9i2.34881.

Full text

Abstract:

Diabetes mellitus has become a worldwide burden due to its persistent, chronic hyperglycemia. D-allulose, a monosaccharide sugar with a 180.16 molecular weight, is widely used as a low-calorie sweetener, is not involved in glucose-related metabolism, and thus does not alter insulin and pancreatic function. This study aimed to evaluate the potential role of honey-derived D-allulose in acute and sub-chronic diabetes mellitus. Diabetic Sprague-Dawley rats were divided into 9 groups and treated with 0.1, 0.2, and 0.4 g/kg BW honey-derived D-allulose for 28, 56, and 84 days. Post-prandial blood glu

APA, Harvard, Vancouver, ISO, and other styles

12

Maeng, Yoon, Chun, et al. "Metabolic Stability of D-Allulose in Biorelevant Media and Hepatocytes: Comparison with Fructose and Erythritol." Foods 8, no. 10 (2019): 448. http://dx.doi.org/10.3390/foods8100448.

Full text

Abstract:

D-allulose, a C-3 epimer of D-fructose, is a rare monosaccharide used as a food ingredient or a sweetener. In the present study, the in vitro metabolic stability of D-allulose was examined in biorelevant media, that is, simulated gastric fluid (SGF) and fasted state simulated intestinal fluid (FaSSIF) containing digestive enzymes, and in cryopreserved human and rat hepatocytes. The hepatocyte metabolic stabilities of D-allulose were also investigated and compared with those of fructose and erythritol (a sugar-alcohol with no calorific value). D-allulose was highly stable in SGF (97.8% remained

APA, Harvard, Vancouver, ISO, and other styles

13

Liu, Bingyang, Yang Gou, Takamasa Tsuzuki, et al. "d-Allulose Improves Endurance and Recovery from Exhaustion in Male C57BL/6J Mice." Nutrients 14, no. 3 (2022): 404. http://dx.doi.org/10.3390/nu14030404.

Full text

Abstract:

d-Allulose, a rare sugar, improves glucose metabolism and has been proposed as a candidate calorie restriction mimetic. This study aimed to investigate the effects of d-allulose on aerobic performance and recovery from exhaustion and compared them with the effects of exercise training. Male C57BL/6J mice were subjected to exercise and allowed to run freely on a wheel. Aerobic performance was evaluated using a treadmill. Glucose metabolism was analyzed by an intraperitoneal glucose tolerance test (ipGTT). Skeletal muscle intracellular signaling was analyzed by Western blotting. Four weeks of da

APA, Harvard, Vancouver, ISO, and other styles

14

Matsuo, Tatsuhiro, Shunsuke Higaki, Reiko Inai, Susumu Mochizuki, Akihide Yoshihara, and Kazuya Akimitsu. "Effect of simultaneous intake of rare sugars allitol and D-allulose on intra-abdominal fat accumulation in rats." Journal of Food Technology Research 10, no. 2 (2023): 37–46. http://dx.doi.org/10.18488/jftr.v10i2.3410.

Full text

Abstract:

Allitol, one of the sugar alcohols, is a rare sugar produced by reducing d-allulose contained in Itea, a deciduous shrub belonging to the Saxifrageaceae family. We previously found that the long-term feeding of rats with a diet supplemented with Itea powder suppressed obesity. The present study aimed to further investigate whether this effect on body fat accumulation may be attributed to the simultaneous intake of allitol and d-allulose in Itea. Thirty-two male 3-week-old Wistar rats were randomly divided into four groups of eight. The rats had ad libitum access to the control (C), 3% allitol

APA, Harvard, Vancouver, ISO, and other styles

15

Xie, Xiaofang, Zihang Jiang, Shixia Xi, Liyuan Jiang, Dejian Huang, and Zhaofeng Li. "Secreted d-allulose 3-epimerase for rare d-allulose biosynthesis and yeast-assisted isomer removal from d-allulose." Food Bioscience 59 (June 2024): 104233. http://dx.doi.org/10.1016/j.fbio.2024.104233.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Han, Youngji, Bo Choi, So Kim, et al. "Gastrointestinal Tolerance of D-Allulose in Healthy and Young Adults. A Non-Randomized Controlled Trial." Nutrients 10, no. 12 (2018): 2010. http://dx.doi.org/10.3390/nu10122010.

Full text

Abstract:

D-allulose has recently received attention as a sugar substitute. However, there are currently no reports regarding its association with gastrointestinal (GI) tolerance. Thus, we performed a GI tolerance test for D-allulose in order to establish its daily acceptable intake level. When the dose of D-allulose was gradually increased in steps of 0.1 g/kg·Body Weight (BW) to identify the maximum single dose for occasional ingestion, no cases of severe diarrhea or GI symptoms were noted until a dose of 0.4 g/kg·BW was reached. Severe symptoms of diarrhea were noted at a dose of 0.5 g/kg·BW. Similar

APA, Harvard, Vancouver, ISO, and other styles

17

Tang, Xinrui, Yingfeng An, Muhammad Waheed Iqbal, et al. "The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production." Foods 11, no. 20 (2022): 3225. http://dx.doi.org/10.3390/foods11203225.

Full text

Abstract:

D-allulose is a natural rare sugar with important physiological properties that is used in food, health care items, and even the pharmaceutical industry. In the current study, a novel D-allulose 3-epimerase gene (Bp-DAE) from the probiotic strain Blautia produca was discovered for the production and characterization of an enzyme known as Bp-DAE that can epimerize D-fructose into D-allulose. Bp-DAE was strictly dependent on metals (Mn2+ and Co2+), and the addition of 1 mM of Mn2+ could enhance the half-life of Bp-DAE at 55 °C from 60 to 180 min. It exhibited optimal activity in a pH of 8 and 55

APA, Harvard, Vancouver, ISO, and other styles

18

Yuma, Tani, Masaaki Tokuda, Naoki Nishimoto, Hideto Yokoi, and Ken Izumori. "Allulose for the attenuation of postprandial blood glucose levels in healthy humans: A systematic review and meta-analysis." PLOS ONE 18, no. 4 (2023): e0281150. http://dx.doi.org/10.1371/journal.pone.0281150.

Full text

Abstract:

D-Allulose is a rare sugar that exists in nature. It is a food ingredient with nearly zero calories (<0.4 kcal/g) and has many physiological functionalities such as attenuation of postprandial blood glucose levels, attenuation of postprandial fat mass accumulation, and anti-aging property. This study focused on the postprandial blood glucose changes in healthy humans by a systematic review and meta-analysis. They were chosen because of its importance to a prevention from diabetes. The study objective was to examine acute blood glucose concentrations of healthy humans after the meal with and

APA, Harvard, Vancouver, ISO, and other styles

19

Cui, Jiawei, Yan Li, and Ming Yan. "Thermostable D-Allulose 3-Epimerase for Long-Term Food-Compatible Continuous Production Systems." Applied Sciences 15, no. 13 (2025): 7318. https://doi.org/10.3390/app15137318.

Full text

Abstract:

D-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits. In this study, we systematically investigated a thermostable D-allulose 3-epimerase (TcDAEase) from Thermogemmatispora carboxidivorans for food-compatible continuous production. The enzyme exhibited remarkable thermostability, with over 70% activity retained at 80 °C, and showed broad pH tolerance across the range of 8.0 to 13.0. Notably, TcDAEase exhibited high catalytic activity toward D-allulose and D-fructose even without the addition of metal i

APA, Harvard, Vancouver, ISO, and other styles

20

Sa, Soonok, Yunji Seol, Albert W. Lee, Yong Heo, Hye-jung Kim, and Chong Jin Park. "Teratogenicity of D-allulose." Toxicology Reports 9 (2022): 821–24. http://dx.doi.org/10.1016/j.toxrep.2022.03.028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Bae, Heekyong R., Su-Kyung Shin, Youngji Han, et al. "D-Allulose Ameliorates Dysregulated Macrophage Function and Mitochondrial NADH Homeostasis, Mitigating Obesity-Induced Insulin Resistance." Nutrients 15, no. 19 (2023): 4218. http://dx.doi.org/10.3390/nu15194218.

Full text

Abstract:

D-allulose, a rare sugar, has been proposed to have potential benefits in addressing metabolic disorders such as obesity and type 2 diabetes (T2D). However, the precise mechanisms underlying these effects remain poorly understood. We aimed to elucidate the mechanisms by which D-allulose influences obesity-induced insulin resistance. We conducted gene set enrichment analysis on the liver and white adipose tissue of mice exposed to a high-fat diet (HFD) along with the white adipose tissue of individuals with obesity. Our study revealed that D-allulose effectively suppressed IFN-γ, restored chemo

APA, Harvard, Vancouver, ISO, and other styles

22

Fukunaga, Kensaku, Takafumi Yoshimura, Hitomi Imachi, et al. "A Pilot Study on the Efficacy of a Diabetic Diet Containing the Rare Sugar D-Allulose in Patients with Type 2 Diabetes Mellitus: A Prospective, Randomized, Single-Blind, Crossover Study." Nutrients 15, no. 12 (2023): 2802. http://dx.doi.org/10.3390/nu15122802.

Full text

Abstract:

High sugar consumption increases the risk of diabetes, obesity, and cardiovascular diseases. Regarding the diet of patients with diabetes, artificial sweeteners are considered a safe alternative to sugar; however, there is also a risk that artificial sweeteners exacerbate glucose metabolism. D-allulose (C-3 isomer of d-fructose), which is a rare sugar, has been reported to have antidiabetic and antiobesity effects. In this study, the efficacy of a diabetic diet containing D-allulose was investigated in patients with type 2 diabetes using an intermittently scanned continuous glucose monitoring

APA, Harvard, Vancouver, ISO, and other styles

23

Do, Ga Young, Eun-Young Kwon, Yun Jin Kim, et al. "Supplementation of Non-Dairy Creamer-Enriched High-Fat Diet with D-Allulose Ameliorated Blood Glucose and Body Fat Accumulation in C57BL/6J Mice." Applied Sciences 9, no. 13 (2019): 2750. http://dx.doi.org/10.3390/app9132750.

Full text

Abstract:

D-allulose, which has 70% of the sweet taste of sucrose but nearly no calories, has been reported to inhibit the absorption of lipids and suppress body weight gain in obese mice. Fats in non-dairy creamer consist of highly saturated fatty acids, which can cause various lipid disorders when consumed over a long period. We investigated whether D-allulose supplementation alleviates the effects of a non-dairy creamer-enriched high-fat diet on lipid metabolism. High-fat diets enriched with non-dairy creamer were administered to C57BL/6J mice with or without D-allulose supplementation for eight week

APA, Harvard, Vancouver, ISO, and other styles

24

Gou, Yang, Bingyang Liu, Mengyao Cheng, et al. "d-Allulose Ameliorates Skeletal Muscle Insulin Resistance in High-Fat Diet-Fed Rats." Molecules 26, no. 20 (2021): 6310. http://dx.doi.org/10.3390/molecules26206310.

Full text

Abstract:

Background: d-Allulose is a rare sugar with antiobesity and antidiabetic activities. However, its direct effect on insulin sensitivity and the underlying mechanism involved are unknown. Objective: This study aimed to investigate the effect of d-allulose on high-fat diet (HFD)-induced insulin resistance using the hyperinsulinemic–euglycemic (HE)-clamp method and intramuscular signaling analysis. Methods: Wistar rats were randomly divided into three dietary groups: chow diet, HFD with 5% cellulose (HFC), and HFD with 5% d-allulose (HFA). After four weeks of feeding, the insulin tolerance test (I

APA, Harvard, Vancouver, ISO, and other styles

25

Teysseire, Fabienne, Valentine Bordier, Aleksandra Budzinska, et al. "Metabolic Effects and Safety Aspects of Acute D-allulose and Erythritol Administration in Healthy Subjects." Nutrients 15, no. 2 (2023): 458. http://dx.doi.org/10.3390/nu15020458.

Full text

Abstract:

The rapid increase in sugar consumption is associated with various negative metabolic and inflammatory effects; therefore, alternative sweeteners become of interest. The aim of this study was to investigate the metabolic effects and safety aspects of acute D-allulose and erythritol on glucose, insulin, ghrelin, blood lipids, uric acid, and high-sensitive C-reactive protein (hsCRP). In three study visits, 18 healthy subjects received an intragastric administration of 25 g D-allulose or 50 g erythritol, or 300 mL tap water (placebo) in a randomized, double-blind and crossover order. To measure t

APA, Harvard, Vancouver, ISO, and other styles

26

Liu, Fawei, Shuangjiang Chen, Fuxu Pan, Zhihui Zhao, Mengjun Liu, and Lili Wang. "Establishment of the Biotransformation of D-Allulose and D-Allose Systems in Full-Red Jujube Monosaccharides." Plants 12, no. 17 (2023): 3084. http://dx.doi.org/10.3390/plants12173084.

Full text

Abstract:

In order to reduce sucrose content in jujube juice and prepare a jujube juice beverage rich in rare sugars, jujube juice was used as raw material for multienzyme catalysis in this study. The effects of single factors such as substrate, pH, DPE and L-RI addition ratio, enzyme treatment temperature, and metal ions on sucrose conversion and D-allulose formation in jujube juice were investigated. Changes in glucose, D-allulose, and D-allose contents in jujube juice before and after enzyme conversion were analyzed by high-performance liquid chromatography (HPLC). The results showed that ‘Xiangfenmu

APA, Harvard, Vancouver, ISO, and other styles

27

Yoshida, Hiromi, Akihide Yoshihara, Pushpa Kiran Gullapalli, et al. "X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose." Acta Crystallographica Section F Structural Biology Communications 74, no. 10 (2018): 669–76. http://dx.doi.org/10.1107/s2053230x18011706.

Full text

Abstract:

The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 Å resolution. The crystal belonged to the hexagonal space group P6522, with unit-cell parameters a = b = 103.98, c = 256.53 Å. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. T

APA, Harvard, Vancouver, ISO, and other styles

28

Franchi, Francesco, Dmitry M. Yaranov, Fabiana Rollini, et al. "Effects of D-allulose on glucose tolerance and insulin response to a standard oral sucrose load: results of a prospective, randomized, crossover study." BMJ Open Diabetes Research & Care 9, no. 1 (2021): e001939. http://dx.doi.org/10.1136/bmjdrc-2020-001939.

Full text

Abstract:

IntroductionCurrent dietary guidelines recommend limiting sugar intake for the prevention of diabetes mellitus (DM). Reduction in sugar intake may require sugar substitutes. Among these, D-allulose is a non-calorie rare monosaccharide with 70% sweetness of sucrose, which has shown anti-DM effects in Asian populations. However, there is limited data on the effects of D-allulose in other populations, including Westerners.Research design and methodsThis was a prospective, randomized, double-blind, placebo-controlled, crossover study conducted in 30 subjects without DM. Study participants were giv

APA, Harvard, Vancouver, ISO, and other styles

29

Li, Jin, Jiajun Chen, Wei Xu, Wenli Zhang, Yeming Chen, and Wanmeng Mu. "Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol." Foods 11, no. 22 (2022): 3613. http://dx.doi.org/10.3390/foods11223613.

Full text

Abstract:

Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehy

APA, Harvard, Vancouver, ISO, and other styles

30

Bu, Yifan, Tao Zhang, Bo Jiang, and Jingjing Chen. "Improved Performance of D-Psicose 3-Epimerase by Immobilisation on Amino-Epoxide Support with Intense Multipoint Attachment." Foods 10, no. 4 (2021): 831. http://dx.doi.org/10.3390/foods10040831.

Full text

Abstract:

D-allulose is an epimer of D-fructose at the C-3 position. With similar sweetness to sucrose and a low-calorie profile, D-allulose has been considered a promising functional sweetener. D-psicose 3-epimerase (DPEase; EC 5.1.3.30) catalyses the synthesis of D-allulose from D-fructose. Immobilised enzymes are becoming increasingly popular because of their better stability and reusability. However, immobilised DPEase generally exhibits less activity or poses difficulty in separation. This study aimed to obtain immobilised DPEase with high catalytic activity, stability, and ease of separation from

APA, Harvard, Vancouver, ISO, and other styles

31

Ivanova, N. S., A. A. Kulminskaya, and S. V. Shvetsova. "Structural and Functional Features of Ketoso-3-Epimerases and Their Use in Production of D-Allulose." Биоорганическая химия 49, no. 4 (2023): 348–59. http://dx.doi.org/10.31857/s0132342323040346.

Full text

Abstract:

Rare sugars attract more and more attention as safe, low-calorie sweeteners and functional compounds in the food, pharmaceutical and medical industries. The potential of the rare sugar D-allulose has been proven in a large number of theoretical and applied works but the high cost of its production is a limitation factor for its large-scall production. Epimerization reactions of available sugars leading to the production of D-allulose are catalyzed by enzymes consisting the epimerase group, namely, ketose-3-epimerases. The key goals of ongoing studies on the ketose-3-epimerase family enzymes ar

APA, Harvard, Vancouver, ISO, and other styles

32

Liu, Zhanzhi, Shuhan Liu, Jingyi Jia, et al. "Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase." Biomolecules 12, no. 11 (2022): 1547. http://dx.doi.org/10.3390/biom12111547.

Full text

Abstract:

d-Allulose is the corresponding epimer of d-fructose at the C-3 position, which exhibits a similar taste and sweetness to sucrose. As a low-calorie sweetener, d-allulose has broad application prospects in the fields of medicine, food, and so on. Currently, the production method of d-allulose is mainly the enzymatic conversion of d-fructose by d-allulose 3-epimerase (DAEase). However, the limited specific activity and thermal stability of DAEase restrict its industrial application. Herein, an ultrahigh-throughput screening assay based on the transcription factor PsiR was extensively optimized f

APA, Harvard, Vancouver, ISO, and other styles

33

Kamal, Shahriar, Yang Gou, Takamasa Tsuzuki, et al. "d-Allulose Ameliorates Fructose-Induced Skeletal Muscle Insulin Resistance via Regulation of Ectopic Lipid Accumulation Independent of Body Weight Changes." Nutrients 17, no. 12 (2025): 2050. https://doi.org/10.3390/nu17122050.

Full text

Abstract:

Background/Objectives: The consumption of fructose-sweetened beverages, especially when combined with a high-fat (HF) diet, substantially contributes to obesity, diabetes, and metabolic dysfunction-associated steatotic liver disease. Ectopic fat accumulation in skeletal muscles is a critical factor in the development of insulin resistance, a key feature of these metabolic disorders. We aimed to investigate the effects of the rare sugar, d-allulose, on fructose-induced insulin resistance. Methods: Male Wistar rats were randomly assigned to fructose-free control diet (CD), HF/fructose-free diet

APA, Harvard, Vancouver, ISO, and other styles

34

Lischer, Kenny, Fina Amreta Laksmi, Yudhi Nugraha, et al. "Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application." PLOS One 20, no. 7 (2025): e0327420. https://doi.org/10.1371/journal.pone.0327420.

Full text

Abstract:

D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute sucrose. This work focused on the development of an economical, scalable production method of DAEase by using the Escherichia coli BL21 star™ (DE3) as host expression. The research work aims to optimize the production of the enzyme through an auto-induction strategy in chemically defined media by using lactose as a natural inducer, thereby overcomi

APA, Harvard, Vancouver, ISO, and other styles

35

Xu, Kang. "Research on Probiotics and D-allulose." International Journal of Biology and Life Sciences 2, no. 2 (2023): 32–34. http://dx.doi.org/10.54097/ijbls.v2i2.6427.

Full text

Abstract:

D-allulose has attracted much attention because of its special taste and unique physiological functions, and it can be used as an ideal substitute for sucrose. At present, probiotics have been proven to have a variety of excellent physiological functions and are widely used in various fields.

APA, Harvard, Vancouver, ISO, and other styles

36

Xiao, Ziqun, Bo Jiang, Jingjing Chen, Longbei Xiang, and Ran Zhang. "Immobilization and stabilization of D-allulose 3-epimerase for continuous D-allulose synthesis in packed-bed reactors." Food Bioscience 64 (February 2025): 105959. https://doi.org/10.1016/j.fbio.2025.105959.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

ÖZGÜR, Mustafa, Elif Büşra ÖZGÜR, and Ahmet Hulusi DİNÇOĞLU. "Prebiotic Effect of D-Allulose (D-Psicose): Traditional Review." Turkiye Klinikleri Journal of Health Sciences 7, no. 2 (2022): 573–77. http://dx.doi.org/10.5336/healthsci.2021-84078.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Az-Zahra, Fauziah, Dwi Hilda Putri, Irdawati Irdawati, and Dezi Handayani. "Literatur Review: D-Allulose 3-Epimerase from Microbial Sources and its Potential Uses." Jurnal Biologi Tropis 25, no. 1 (2025): 795–800. https://doi.org/10.29303/jbt.v25i1.8402.

Full text

Abstract:

D-allulose, or psicose, is a rare, low-calorie sugar with various health benefits, such as managing blood sugar levels and lowering the risk of obesity, making it an ideal alternative to sucrose. In the food and pharmaceutical sectors, this study aims to review the characteristics and potential of D-allulose 3-epimerase (DAEase) enzymes from different microbial sources, along with the challenges and prospects associated with industrial-scale applications. The methodology involved a literature review to analyse the properties of DAEase enzymes, including specific activity, thermal stability, an

APA, Harvard, Vancouver, ISO, and other styles

39

Masuda, Yuta, Kento Ohbayashi, Kengo Iba, et al. "Abilities of Rare Sugar Members to Release Glucagon-like Peptide-1 and Suppress Food Intake in Mice." Nutrients 17, no. 7 (2025): 1221. https://doi.org/10.3390/nu17071221.

Full text

Abstract:

Background/Objectives: Rare sugars, which naturally exist in small quantities, have gained attention as next-generation functional sugars due to their sweetness and low calorie content. Some of them have already been commercialized. Rare sugar-containing syrups, produced through alkaline isomerization of high-fructose corn syrup, are effective in preventing obesity and type 2 diabetes. However, the mechanisms underlying these effects remain incompletely understood. Recently, D-allulose has been found to improve hyperphagic obesity by stimulating the secretion of the intestinal hormone glucagon

APA, Harvard, Vancouver, ISO, and other styles

40

Wang, Lei, Yun Cui, Yujie Lu, and Zongpei Zhao. "Comprehensive Analysis of Allulose Production: A Review and Update." Foods 13, no. 16 (2024): 2572. http://dx.doi.org/10.3390/foods13162572.

Full text

Abstract:

Advancements in D-allulose production have seen significant strides in recent years, focusing on enzymatic conversion methods. Key developments include traditional immobilization techniques, the discovery of novel enzymes, directed evolution studies, and biosynthesis through metabolic pathway modification. Enzymatic conversion, particularly utilizing D-allulose 3-epimerase, remains fundamental for industrial-scale production. Innovative immobilization strategies, such as functionalized nano-beads and magnetic MOF nanoparticles, have significantly enhanced enzyme stability and reusability. Dire

APA, Harvard, Vancouver, ISO, and other styles

41

Chen, Jiajun, Ding Chen, Mengyu Ke, et al. "Characterization of a Recombinant d-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in d-Allulose Production." Molecular Biotechnology 63, no. 6 (2021): 534–43. http://dx.doi.org/10.1007/s12033-021-00320-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Jia, Dong-Xu, Chen-Yi Sun, Yi-Ting Jin, et al. "Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose." Enzyme and Microbial Technology 148 (August 2021): 109816. http://dx.doi.org/10.1016/j.enzmictec.2021.109816.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Park, Chul-Soon, Taeyong Kim, Seung-Hye Hong, Kyung-Chul Shin, Kyoung-Rok Kim, and Deok-Kun Oh. "D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii." PLOS ONE 11, no. 7 (2016): e0160044. http://dx.doi.org/10.1371/journal.pone.0160044.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Suzuki, Yota, Takeshi Hashimoto та Takashi Hayashita. "Ratiometric fluorescence sensing of d-allulose using an inclusion complex of γ-cyclodextrin with a benzoxaborole-based probe". RSC Advances 12, № 19 (2022): 12145–51. http://dx.doi.org/10.1039/d2ra00749e.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Ding, Wentao, Chensa Liu, Chi Huang, et al. "The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability." International Journal of Molecular Sciences 25, no. 12 (2024): 6361. http://dx.doi.org/10.3390/ijms25126361.

Full text

Abstract:

As a low-calorie sugar, D-allulose is produced from D-fructose catalyzed by D-allulose 3-epimerase (DAE). Here, to improve the catalytic activity, stability, and processability of DAE, we reported a novel method by forming organic–inorganic hybrid nanoflowers (NF-DAEs) and co-immobilizing them on resins to form composites (Re-NF-DAEs). NF-DAEs were prepared by combining DAE with metal ions (Co2+, Cu2+, Zn2+, Ca2+, Ni2+, Fe2+, and Fe3+) in PBS buffer, and were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction. All of the NF-DAEs showe

APA, Harvard, Vancouver, ISO, and other styles

46

Kanasaki, Akane, Misato Niibo, and Tetsuo Iida. "Effect of d-allulose feeding on the hepatic metabolomics profile in male Wistar rats." Food & Function 12, no. 9 (2021): 3931–38. http://dx.doi.org/10.1039/d0fo03024d.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Xie, Xiaofang, Yixiong Tian, Xiaofeng Ban, Caiming Li, Hongshun Yang, and Zhaofeng Li. "Crystal structure of a novel homodimeric D-allulose 3-epimerase from a Clostridia bacterium." Acta Crystallographica Section D Structural Biology 78, no. 9 (2022): 1180–91. http://dx.doi.org/10.1107/s2059798322007707.

Full text

Abstract:

D-Allulose, a low-calorie rare sugar with various physiological functions, is mainly produced through the isomerization of D-fructose by ketose 3-epimerases (KEases), which exhibit various substrate specificities. A novel KEase from a Clostridia bacterium (CDAE) was identified to be a D-allulose 3-epimerase and was further characterized as thermostable and metal-dependent. In order to explore its structure–function relationship, the crystal structure of CDAE was determined using X-ray diffraction at 2.10 Å resolution, revealing a homodimeric D-allulose 3-epimerase structure with extensive inte

APA, Harvard, Vancouver, ISO, and other styles

48

Patel, Satya Narayan, Sweety Sharma, Nidhi Gossai, Dhaval Patel, and Sudhir Pratap Singh. "A novel xylose isomerase suitable for D-fructose production and synergistic catalysis with D-allulose 3-epimerase for the biosynthesis of D-allulose." Process Biochemistry 151 (April 2025): 52–64. https://doi.org/10.1016/j.procbio.2025.01.030.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

刘, 侯. "Progress in Production and Purification of D-Allulose." Bioprocess 08, no. 02 (2018): 33–39. http://dx.doi.org/10.12677/bp.2018.82004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Hong, Jong-Hwan, Hack-Youn Kim, Su-Han Kang, et al. "Quality Properties of Chicken Breast Tteokgalbi with D-Allulose and Sprout-Barley." Resources Science Research 2, no. 1 (2020): 18–27. http://dx.doi.org/10.52346/rsr.2020.2.1.18.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!