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Journal articles on the topic 'Data storage into DNA molecules'

Author: Grafiati

Published: 14 December 2024

Last updated: 27 July 2025

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1

Jiang, Jiao. "Application of gene editing technology to DNA digital data storage." Highlights in Science, Engineering and Technology 73 (November 29, 2023): 452–58. http://dx.doi.org/10.54097/hset.v73i.14051.

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While the archival digital storage industry is approaching its physical limits, demand is increasing significantly, so alternatives are emerging. The modern world is in dire need of durable, scalable and economical alternative storage media. Deoxyribonucleic acid (DNA), a promising storage medium, offers superior information durability, capacity and energy consumption, making it a promising candidate for long-term data storage. However, the design and realization of DNA digital data storage face many problems, but gene editing technology, as a technology that makes modifications to genes direc

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2

Garafutdinov, R. R., A. R. Sakhabutdinova, and A. V. Chemeris. "Long-term room temperature storage of DNA molecules." Biomics 12, no. 4 (2020): 552–63. http://dx.doi.org/10.31301/2221-6197.bmcs.2020-49.

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The simplest and most common method of long-term storage of DNA samples at present is the storage of their frozen solutions, which, however, has a number of disadvantages, including the destruction of DNA molecules during freezing and thawing, as well as energy consumption and the likelihood of losing valuable samples in the event of possible accidents. In this regard, long-term storage of DNA samples at room temperature in a dried state is preferable, especially since an even greater increase in the number of stored DNA samples is planned due to the planned preservation of non-biological data

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Zhang, Yun Peng, Feng Ying Tian, Man Hui Sun, Ding Yu, Fei Xiang Fan, and Wei Guo Liu. "Based on DNA OTP Key Generation and Management Research." Applied Mechanics and Materials 427-429 (September 2013): 2470–72. http://dx.doi.org/10.4028/www.scientific.net/amm.427-429.2470.

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With the development of molecular-bio technology, the feature of DNA molecules for ultra-large-scale data storage has created a new approach for data storage. This paper gives a way of strengthening key transport security. Through recombinant DNA technology, use only sender-receiver know restriction enzymes to combine the key DNA and the T vector, to form a recombinant plasmid, making the key DNA bio-hide, and then place the recombinant plasmid in implanted bacteria .

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Ceze, Luis, Jeff Nivala, and Karin Strauss. "Molecular digital data storage using DNA." Nature Reviews Genetics 20, no. 8 (2019): 456–66. http://dx.doi.org/10.1038/s41576-019-0125-3.

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Coudy, Delphine, Marthe Colotte, Aurélie Luis, Sophie Tuffet, and Jacques Bonnet. "Long term conservation of DNA at ambient temperature. Implications for DNA data storage." PLOS ONE 16, no. 11 (2021): e0259868. http://dx.doi.org/10.1371/journal.pone.0259868.

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DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under

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Carmean, Douglas, Luis Ceze, Georg Seelig, Kendall Stewart, Karin Strauss, and Max Willsey. "DNA Data Storage and Hybrid Molecular–Electronic Computing." Proceedings of the IEEE 107, no. 1 (2019): 63–72. http://dx.doi.org/10.1109/jproc.2018.2875386.

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Solanki, Arnav, Zak Griffin, Purab Ranjan Sutradhar, et al. "Neural network execution using nicked DNA and microfluidics." PLOS ONE 18, no. 10 (2023): e0292228. http://dx.doi.org/10.1371/journal.pone.0292228.

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DNA has been discussed as a potential medium for data storage. Potentially it could be denser, could consume less energy, and could be more durable than conventional storage media such as hard drives, solid-state storage, and optical media. However, performing computations on the data stored in DNA is a largely unexplored challenge. This paper proposes an integrated circuit (IC) based on microfluidics that can perform complex operations such as artificial neural network (ANN) computation on data stored in DNA. We envision such a system to be suitable for highly dense, throughput-demanding bio-

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Xu, Chengtao, Chao Zhao, Biao Ma, and Hong Liu. "Uncertainties in synthetic DNA-based data storage." Nucleic Acids Research 49, no. 10 (2021): 5451–69. http://dx.doi.org/10.1093/nar/gkab230.

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Abstract Deoxyribonucleic acid (DNA) has evolved to be a naturally selected, robust biomacromolecule for gene information storage, and biological evolution and various diseases can find their origin in uncertainties in DNA-related processes (e.g. replication and expression). Recently, synthetic DNA has emerged as a compelling molecular media for digital data storage, and it is superior to the conventional electronic memory devices in theoretical retention time, power consumption, storage density, and so forth. However, uncertainties in the in vitro DNA synthesis and sequencing, along with its

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Bhattarai-Kline, Santi, Sierra K. Lear, and Seth L. Shipman. "One-step data storage in cellular DNA." Nature Chemical Biology 17, no. 3 (2021): 232–33. http://dx.doi.org/10.1038/s41589-021-00737-2.

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10

Zhang, Cheng, Ranfeng Wu, Fajia Sun, et al. "Parallel molecular data storage by printing epigenetic bits on DNA." Nature 634, no. 8035 (2024): 824–32. http://dx.doi.org/10.1038/s41586-024-08040-5.

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Organick, Lee, Siena Dumas Ang, Yuan-Jyue Chen, et al. "Random access in large-scale DNA data storage." Nature Biotechnology 36, no. 3 (2018): 242–48. http://dx.doi.org/10.1038/nbt.4079.

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Kumar, ArunH S., and Apostolos Zarros. "Digitization of DNA: Miniaturization of information storage moving toward data in DNA!" Journal of Natural Science, Biology and Medicine 4, no. 1 (2013): 1. http://dx.doi.org/10.4103/0976-9668.107252.

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13

Liu, Wei, Huaichuan Duan, Derong Zhang, et al. "Concepts and Application of DNA Origami and DNA Self-Assembly: A Systematic Review." Applied Bionics and Biomechanics 2021 (November 16, 2021): 1–15. http://dx.doi.org/10.1155/2021/9112407.

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With the arrival of the post-Moore Era, the development of traditional silicon-based computers has reached the limit, and it is urgent to develop new computing technology to meet the needs of science and life. DNA computing has become an essential branch and research hotspot of new computer technology because of its powerful parallel computing capability and excellent data storage capability. Due to good biocompatibility and programmability properties, DNA molecules have been widely used to construct novel self-assembled structures. In this review, DNA origami is briefly introduced firstly. Th

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Kevin, MacVittie, and Kraus Petra. "Development, Practice, and Perspectives for DNA Enzyme Systems." Biohelikon 3, no. 2 (2015): 1–7. https://doi.org/10.5281/zenodo.807692.

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DNAzymes are still fairly recent man-made additions to the growing spectrum of synthetic molecules dramatically advancing the field of biomolecular science. These catalytically active nucleic acids have great potential in personalized medicine and as internal biosensors owing to their low immunogenicity, as well as in bio-computing, because of their robust nature and large data storage capacity. Their future potential is evident in areas that range from the development of biofuel cells to power implanted electronics, like pacemakers, where they could exceed capabilities and circumvent obstacle

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Anavy, Leon, Inbal Vaknin, Orna Atar, Roee Amit, and Zohar Yakhini. "Data storage in DNA with fewer synthesis cycles using composite DNA letters." Nature Biotechnology 37, no. 10 (2019): 1229–36. http://dx.doi.org/10.1038/s41587-019-0240-x.

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Goumy, Carole, Zangbéwendé Guy Ouedraogo, Elodie Bellemonte, et al. "Feasibility of Optical Genome Mapping from Placental and Umbilical Cord Sampled after Spontaneous or Therapeutic Pregnancy Termination." Diagnostics 13, no. 23 (2023): 3576. http://dx.doi.org/10.3390/diagnostics13233576.

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Optical genome mapping (OGM) is an alternative to classical cytogenetic techniques to improve the detection rate of clinically significant genomic abnormalities. The isolation of high-molecular-weight (HMW) DNA is critical for a successful OGM analysis. HMW DNA quality depends on tissue type, sample size, and storage conditions. We assessed the feasibility of OGM analysis of DNA from nine umbilical cord (UC) and six chorionic villus (CV) samples collected after the spontaneous or therapeutic termination of pregnancy. We analyzed quality control metrics provided by the Saphyr system (Bionano Ge

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El-Seoud, Samir Abou, Reham Fouad Mohamed, and Samy Ghoneimy. "DNA Computing: Challenges and Application." International Journal of Interactive Mobile Technologies (iJIM) 11, no. 2 (2017): 74. http://dx.doi.org/10.3991/ijim.v11i2.6564.

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<p class="Abstract">Much of our scientific, technological, and economic future depends on the availability of an ever-increasing supply of computational power. However, the increasing demand for such power has pushed electronic technology to the limit of physical feasibility and has raised the concern that this technology may not be able to sustain our growth in the near future. It became important to consider an alternative means of achieving computational power. In this regard, DNA computing was introduced based on the usage of DNA and molecular biology hardware instead of the typical

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Anavy, Leon, Inbal Vaknin, Orna Atar, Roee Amit, and Zohar Yakhini. "Author Correction: Data storage in DNA with fewer synthesis cycles using composite DNA letters." Nature Biotechnology 37, no. 10 (2019): 1237. http://dx.doi.org/10.1038/s41587-019-0281-1.

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Et. al., Arsha Kolate,. "An Information Security Using DNA Cryptography along with AES Algorithm." Turkish Journal of Computer and Mathematics Education (TURCOMAT) 12, no. 1S (2021): 183–92. http://dx.doi.org/10.17762/turcomat.v12i1s.1607.

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Securing information is the most important need of not only the business world but also it’s highly essential in all the other major sectors. The secured data storage capacity along with security during data transit is also an important factor. In this paper DNA based security technique is proposed as an information carrier, the new data securing method can be adopted by harnessing the advantages of DNA based AES. This technique will provide multilayer security. The proposed system aims to secure transactional data during communication as it is required when message or data transfer between se

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20

Du, Haigui, Shihua Zhou, WeiQi Yan, and Sijie Wang. "Study on DNA Storage Encoding Based IAOA under Innovation Constraints." Current Issues in Molecular Biology 45, no. 4 (2023): 3573–90. http://dx.doi.org/10.3390/cimb45040233.

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With the informationization of social processes, the amount of related data has greatly increased, making traditional storage media unable to meet the current requirements for data storage. Due to its advantages of a high storage capacity and persistence, deoxyribonucleic acid (DNA) has been considered the most prospective storage media to solve the data storage problem. Synthesis is an important process for DNA storage, and low-quality DNA coding can increase errors during sequencing, which can affect the storage efficiency. To reduce errors caused by the poor stability of DNA sequences durin

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Muhammad Zulkifl Hasan, Muhammad Zunnurain Hussain, Zaka Ullah, and Taimoor Hassan. "Future Computing: DNA Hard Drives." Lahore Garrison University Research Journal of Computer Science and Information Technology 3, no. 1 (2019): 31–33. http://dx.doi.org/10.54692/lgurjcsit.2019.030165.

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DNA is a hard drive, the memory in every cell of every living organism that has the instructions on how to make that cell. With the exponential growth of our media, the growth of data storage is also increasing. DNA is incredible compact due to its molecular structure and can be used to achieve data attractively through genome sequencing. A raw limit of 1 Exabyte/mm3 (109 GB/mm3) having half-life of over 500 years. With this, all the data of the world can be stored in just a small place of a room.

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Karakose, Mehmet, and Ugur Cigdem. "QPSO-Based Adaptive DNA Computing Algorithm." Scientific World Journal 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/160687.

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DNA (deoxyribonucleic acid) computing that is a new computation model based on DNA molecules for information storage has been increasingly used for optimization and data analysis in recent years. However, DNA computing algorithm has some limitations in terms of convergence speed, adaptability, and effectiveness. In this paper, a new approach for improvement of DNA computing is proposed. This new approach aims to perform DNA computing algorithm with adaptive parameters towards the desired goal using quantum-behaved particle swarm optimization (QPSO). Some contributions provided by the proposed

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Brasell, Katie A., Xavier Pochon, Jamie Howarth, et al. "Shifts in DNA yield and biological community composition in stored sediment: implications for paleogenomic studies." Metabarcoding and Metagenomics 6 (February 1, 2022): e78128. https://doi.org/10.3897/mbmg.6.78128.

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Lake sediments hold a wealth of information from past environments that is highly valuable for paleolimnological reconstructions. These studies increasingly apply modern molecular tools targeting sedimentary DNA (sedDNA). However, sediment core sampling can be logistically difficult, making immediate subsampling for sedDNA challenging. Sediment cores are often refrigerated (4 °C) for weeks or months before subsampling. We investigated the impact of storage time on changes in DNA (purified or as cell lysate) concentrations and shifts in biological communities following storage of lake surface s

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Lee, Magdelene N., Gunavaran Brihadiswaran, Balaji M. Rao, James M. Tuck, and Albert J. Keung. "Yeast Surface Display of Protein Addresses Confers Robust Storage and Access of DNA-Based Data." DNA 5, no. 3 (2025): 34. https://doi.org/10.3390/dna5030034.

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Background/Objectives: The potential of DNA as an information-dense storage medium has inspired a broad spectrum of creative systems. In particular, hybrid biomolecular systems that integrate new materials and chemistries with DNA could drive novel functions. In this work, we explore the potential for proteins to serve as molecular file addresses. We stored DNA-encoded data in yeast and leveraged yeast surface display to readily produce the protein addresses and make them easy to access on the cell surface. Methods: We generated yeast populations that each displayed a distinct protein on their

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Cao, Ben, Yanfen Zheng, Qi Shao, et al. "Efficient data reconstruction: The bottleneck of large-scale application of DNA storage." Cell Reports 43, no. 4 (2024): 113699. http://dx.doi.org/10.1016/j.celrep.2024.113699.

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26

Köhler, Johann Michael. "Nano-Archives in Soils—What Microbial DNA Molecules Can Report About the History of Places." Applied Nano 6, no. 1 (2025): 2. https://doi.org/10.3390/applnano6010002.

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DNA encoding the 16S rRNA of bacteria is a type of nanometer-sized information storage that can be used to characterize bacterial communities in soils. Reading this molecular ’nano-archive’ is not only of interest for characterizing recent local ecological conditions but can also provide valuable information about human impacts on soils in the past. This is of great interest for archaeology and for understanding the ecological consequences of past human activities on recent ecological conditions. Powerful sequencing methods such as the Illumina process allow many different DNA sequences to be

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Xiao, Xuefeng, Yunuo Song, Jingxuan Hu, Jinchen Han, Wanbin Xing, and Chang Liu. "Coli bond: A dual-function encryption system for secure information storage and transmission by microorganisms." PLOS One 20, no. 6 (2025): e0325926. https://doi.org/10.1371/journal.pone.0325926.

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With global data expected to reach 175 zettabytes by 2025, traditional storage methods face unprecedented challenges, including security risks, limited durability, and high maintenance costs associated with centralized infrastructure. While DNA-based storage systems have demonstrated high density and chemical stability, most existing methods focus primarily on static storage, lacking effective strategies for secure and controllable information transmission. Coli Bond offers a revolutionary approach by combining the molecular precision of DNA storage with the controllable dynamics of synthetic

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Jonas, Wolf. "Molecular data storage with zero synthetic effort and simple read-out." Scientific Reports 12, no. x (2022): x. https://doi.org/10.5281/zenodo.10454503.

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Compound mixtures represent an alternative, additional approach to DNA and synthetic sequence-defined macromolecules in the field of non-conventional molecular data storage, which may be useful depending on the target application. Here, we report a fast and efficient method for information storage in molecular mixtures by the direct use of commercially available chemicals and thus, zero synthetic steps need to be performed. As a proof of principle, a binary coding language is used for encoding words in ASCII or black and white pixels of a bitmap. This way, we stored a 25 × 

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Jiang, Bin, Yikun Zhao, Hongmei Yi, et al. "PIDS: A User-Friendly Plant DNA Fingerprint Database Management System." Genes 11, no. 4 (2020): 373. http://dx.doi.org/10.3390/genes11040373.

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The high variability and somatic stability of DNA fingerprints can be used to identify individuals, which is of great value in plant breeding. DNA fingerprint databases are essential and important tools for plant molecular research because they provide powerful technical and information support for crop breeding, variety quality control, variety right protection, and molecular marker-assisted breeding. Building a DNA fingerprint database involves the production of large amounts of heterogeneous data for which storage, analysis, and retrieval are time and resource consuming. To process the larg

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Yu, Meng, Xiaohui Tang, Zhenhua Li, et al. "High-throughput DNA synthesis for data storage." Chemical Society Reviews, 2024. http://dx.doi.org/10.1039/d3cs00469d.

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Using DNA molecules for digital data storage: the writing and reading of the data are realized by high throughput DNA synthesis and sequencing technologies, where high density array-based chips play an important role.

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Liu, Bingyi, Fei Wang, Chunhai Fan, and Qian Li. "Data Readout Techniques for DNA‐Based Information Storage." Advanced Materials, February 5, 2025. https://doi.org/10.1002/adma.202412926.

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AbstractDNA is a natural chemical substrate that carries genetic information, which also serves as a powerful toolkit for storing digital data. Compared to traditional storage media, DNA molecules offer higher storage density, longer lifespan, and lower maintenance energy consumption. In DNA storage process, data readout is a critical step that bridges the gap between DNA molecular/structures with stored digital information. With the continued development of strategies in DNA data storage technology, the readout techniques have evolved. However, there is a lack of systematic introduction and d

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MANAR, SAIS NAJAT RAFALIA JAAFAR ABOUCHABAKA. "SYNTHETIC DNA AS A SOLUTION TO THE BIG DATA STORAGE PROBLEM." Journal of Theoretical and Applied Information Technology 99, no. 15 (2021). https://doi.org/10.5281/zenodo.5353710.

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In the last few years, we have witnessed unprecedented growth in data and gigantic amounts of data are being produced every day. By 2020, the amount of information we want to store it will be around 44 trillion gigabytes. on the one hand the data volumes continue to grow at an even higher speed, However, our traditional databases are limited in the storage and processing of this large and complex data and we do not have a reliable physical storage medium that can withstand the weather. On the other hand, the term Big Data is now the new natural resource and current analysis architectures face

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Zhang, Lichao, Yuanyuan Lv, Lei Xu, and Murong Zhou. "A review of DNA data storage technologies based on biomolecules." Current Bioinformatics 16 (August 13, 2021). http://dx.doi.org/10.2174/1574893616666210813101237.

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: In the information age, data storage technology has become the key to improving computer systems. Since traditional storage technologies cannot meet the demand for massive storage, new DNA storage technology based on biomolecules attracts much attentions. DNA storage refers to the technology that uses artificially synthesized deoxynucleotide chains to store and read all information, such as documents, pictures, and audio. First, data are encoded into binary number strings. Then, the four types of base, A(Adenine), T(Thymine), C(Cytosine), and G(Guanine), are used to encode the corresponding

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Doricchi, A., C. M. Platnich, A. Gimpel, et al. "Emerging Approaches to DNA Data Storage: Challenges and Prospects." October 11, 2022. https://doi.org/10.1021/acsnano.2c06748.

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With the total amount of worldwide data skyrocketing, the global data storage demand is predicted to grow to 1.75 × 1014 GB by 2025. Traditional storage methods have difficulties keeping pace given that current storage media have a maximum density of 103 GB/mm3. As such, data production will far exceed the capacity of currently available storage methods. The costs of maintaining and transferring data, as well as the limited lifespans and significant data losses associated with current technologies also demand advanced solutions for information storage. Nature offers a powerful alternativ

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Ma, Jiaxiang, Yu Yang, Ben Pei, Shengli Mi, Zhuo Xiong, and Liliang Ouyang. "Primer‐Disk‐Enabled DNA Data Storage System with Index and Record‐Many‐Read‐Many Features." Advanced Science, June 4, 2025. https://doi.org/10.1002/advs.202502367.

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AbstractDNA data storage has emerged as a promising information storage technology by encoding information down to base molecules. However, it remains a challenge to structure the DNA data with ease of recording, retrieving, and reading. Here, a primer‐disk‐enabled hierarchical DNA data storage system is introduced, which allows for the multiple immobilizations of DNA molecules and the generation of corresponding QR codes for retrieving. The primer disk is pre‐engineered to present multiple primers, on which encoded DNA molecules with complementary primers can be covalently immobilized on dema

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Fan, Qingyuan. "De Novo Non-Canonical Nanopore Basecalling Enables Private Communication using Heavily-modified DNA Data at Single-Molecule Level." March 22, 2025. https://doi.org/10.5281/zenodo.12704171.

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This repostry contains the sequencing pod5 files from 5hmC-modified 6-mer QC sequences, Bacterial Genome DNA (gDNA) sequences, DNA Storage sequences for training the 5hmC DeepSME basecaller and conducting the DNA storage decoding experiments. File Description 5hmc_pod5_for_training_reinforced_deepsme.zip pod5 file for training the 5hmC reinforced DeepSME, reference FASTA can be retrieved from NCBI ( Pseudomonas_aeruginosa_PAO1 (https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000006765.1/ ), Aeromonas_hydrophila_BJ054 (https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_046708825.1/ ), and Vibrio

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Sriram.S and Dr. D. R. Krithika. "DNA as a Storage Medium for Efficient and Reliable Cloud Data Archieving." International Journal of Advanced Research in Science, Communication and Technology, July 4, 2024, 93–100. http://dx.doi.org/10.48175/ijetir-1218.

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On Earth right now, there are about 10 trillion gigabytes of digital data, and every day, humans produce emails, photos, tweets, and other digital files that add up to another 2.5 million gigabytes of data.Much of this data is stored in enormous facilities known as exabyte data centers (an exabyte is 1 billion gigabytes), which can be the size of several football fields and cost around $1 billion to build and maintain.Demand for data storage is growing exponentially, but the capacity of existing storage media is not keeping up.This project enables molecular-level data storage into DNA molecule

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Tomek, Kyle J., Kevin Volkel, Elaine W. Indermaur, James M. Tuck, and Albert J. Keung. "Promiscuous molecules for smarter file operations in DNA-based data storage." Nature Communications 12, no. 1 (2021). http://dx.doi.org/10.1038/s41467-021-23669-w.

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AbstractDNA holds significant promise as a data storage medium due to its density, longevity, and resource and energy conservation. These advantages arise from the inherent biomolecular structure of DNA which differentiates it from conventional storage media. The unique molecular architecture of DNA storage also prompts important discussions on how data should be organized, accessed, and manipulated and what practical functionalities may be possible. Here we leverage thermodynamic tuning of biomolecular interactions to implement useful data access and organizational features. Specific sets of

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Takahashi, Christopher N., David P. Ward, Carlo Cazzaniga, et al. "Evaluating the risk of data loss due to particle radiation damage in a DNA data storage system." Nature Communications 15, no. 1 (2024). http://dx.doi.org/10.1038/s41467-024-51768-x.

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AbstractDNA data storage is a potential alternative to magnetic tape for archival storage purposes, promising substantial gains in information density. Critical to the success of DNA as a storage media is an understanding of the role of environmental factors on the longevity of the stored information. In this paper, we evaluate the effect of exposure to ionizing particle radiation, a cause of data loss in traditional magnetic media, on the longevity of data in DNA data storage pools. We develop a mass action kinetics model to estimate the rate of damage accumulation in DNA strands due to neutr

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Bar-Lev, Daniella, Inbal Preuss, Leon Anavy, and Eitan Yaakobi. "Storing Big Data in Tiny DNA Molecules." Frontiers for Young Minds 13 (June 5, 2025). https://doi.org/10.3389/frym.2025.1579129.

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How many pictures did you take this week? Did you watch videos on YouTube, or maybe a series on Netflix? All of these activities rely on digital information stored on your smartphone, computer, or in the cloud, ready for you to access whenever you want. As people create more and more data, the technology we use to store it has to change to handle new and challenging problems. One promising solution is the use of synthetic DNA molecules. DNA is a natural material found in most cells of all living organisms, and it stores the organism’s genetic information—huge amounts of biological data. Today,

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Kryuchyn, A., Ye V. Belyak, Ye A. Kryuchyna та A. V. Potebnya. "СТАН І ПРОБЛЕМИ СТВОРЕННЯ ДНК-ПАМ'ЯТІ". Medical Informatics and Engineering, № 3 (20 жовтня 2015). http://dx.doi.org/10.11603/mie.1996-1960.2015.3.4997.

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The results of the analysis of the development of storage systems on DNA molecules are given. The considerable potential for such storage systems for the organization of long-term storage of large volumes of information is shown. The conditions for the extensive use of memory on DNA are determined. It is shown that the key to the use of WORM-type memory on DNA molecules is a significant increase in the speed of sequencing nukleatidov recorded sequences. Data on conditions of storage memory chips on DNA, which provide long-term storage of large volumes of information is presented.

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Matange, Karishma, James M. Tuck, and Albert J. Keung. "DNA stability: a central design consideration for DNA data storage systems." Nature Communications 12, no. 1 (2021). http://dx.doi.org/10.1038/s41467-021-21587-5.

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AbstractData storage in DNA is a rapidly evolving technology that could be a transformative solution for the rising energy, materials, and space needs of modern information storage. Given that the information medium is DNA itself, its stability under different storage and processing conditions will fundamentally impact and constrain design considerations and data system capabilities. Here we analyze the storage conditions, molecular mechanisms, and stabilization strategies influencing DNA stability and pose specific design configurations and scenarios for future systems that best leverage the

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Bajc, Gregor, Anja Pavlin, Małgorzata Figiel, Weronika Zajko, Marcin Nowotny, and Matej Butala. "Data storage based on the absence of nucleotides using a bacteriophage abortive infection system reverse transcriptase." Lab on a Chip, 2024. http://dx.doi.org/10.1039/d4lc00755g.

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DNA molecules are a promising data storage medium for the future; however, effective de novo synthesis of DNA using an enzyme that catalyzes the polymerization of natural nucleoside triphosphates in...

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Chen, Yuan-Jyue, Christopher N. Takahashi, Lee Organick, et al. "Quantifying molecular bias in DNA data storage." Nature Communications 11, no. 1 (2020). http://dx.doi.org/10.1038/s41467-020-16958-3.

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Volkel, Kevin D., Kevin N. Lin, Paul W. Hook, Winston Timp, Albert J. Keung, and James M. Tuck. "FrameD: Framework for DNA-based Data Storage Design, Verification, and Validation." Bioinformatics, September 15, 2023. http://dx.doi.org/10.1093/bioinformatics/btad572.

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Abstract Motivation DNA-based data storage is a quickly growing field that hopes to harness the massive theoretical information density of DNA molecules to produce a competitive next-generation storage medium suitable for archival data. In recent years, many DNA-based storage system designs have been proposed. Given that no common infrastructure exists for simulating these storage systems, comparing many different designs along with many different error models is increasingly difficult. To address this challenge we introduce FrameD, a simulation infrastructure for DNA storage systems that leve

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Lee, Howon, Daniel J. Wiegand, Kettner Griswold, et al. "Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage." Nature Communications 11, no. 1 (2020). http://dx.doi.org/10.1038/s41467-020-18681-5.

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Abstract New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co2+ ions by patterned UV li

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Liu, Deruilin, Demin Xu, Liuxin Shi, et al. "A practical DNA data storage using an expanded alphabet introducing 5-methylcytosine." Gigabyte 2025 (January 24, 2025). https://doi.org/10.46471/gigabyte.147.

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The DNA molecule is a promising next-generation data storage medium. Recently, it has been theoretically proposed that non-natural or modified bases can serve as extra molecular letters to increase the information density. However, this strategy is challenging due to the difficulty in synthesizing non-natural DNA sequences and their complex structure. Here, we described a practical DNA data storage transcoding scheme named R+ based on an expanded molecular alphabet that introduces 5-methylcytosine (5mC). We demonstrated its experimental validation by encoding one representative file into sever

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Leblanc, Julien, Olivier Boulle, Emeline Roux, Jacques Nicolas, Dominique Lavenier, and Yann Audic. "Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information." BioTechniques, April 4, 2024. http://dx.doi.org/10.2144/btn-2023-0109.

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In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to

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Coudy, Delphine, Marthe Colotte, Aurélie Luis, Sophie Tuffet, and Jacques Bonnet. "Long Term Conservation of DNA at Ambient Temperature. Implications for DNA Data Storage." Applied Cell Biology 10, no. 1 (2022). http://dx.doi.org/10.53043/2320-1991.acb90017.

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DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under

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SUNEJA, VAIBHAV. "BIG DATA MANAGEMENT – DNA DATA WAREHOUSE." International Journal of Computer and Communication Technology, July 2015, 170–73. http://dx.doi.org/10.47893/ijcct.2015.1298.

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Big data storage and retrieval is cause of concern for future. This paper proposes to store data by organizing nucleotides (adenine (A) and thymine (T)) to represent binary 0’s and 1’s. Small fragments of high molecular DNA can be achieved by chain termination method and by shotgun sequencing method, we can select the fragments containing A’s and T’s in order we want. This paper demonstrates a python script, which can produce A and T sequence from the sequence of data as input which can be used while using shotgun sequencing for selecting/discarding of strands, script can also be used to query

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