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Journal articles on the topic 'Date Palm Callus Initiation Medium'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Azad, Md Abul Kalam, Hasnatul Arefin, and Md Amzad Hossain. "In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)." Plant Tissue Culture and Biotechnology 23, no. 2 (2014): 211–19. http://dx.doi.org/10.3329/ptcb.v23i2.17522.

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After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D

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2

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, and Snehasis Jana. "Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium." International Journal of Nutrition and Food Science 4, no. 6 (2015): 660–68. https://doi.org/10.11648/j.ijnfs.20150406.20.

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The date palm is mainly cultivated for the production of sweet fruit. Date palm callus initiation medium (DPCIM) is used for plant tissue culture applications. The present work is intended to evaluate the impact of Mr. Trivedi’s biofield energy treatment on physical, thermal and spectral properties of the DPCIM. The control and treated DPCIM were evaluated by various analytical techniques such as X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer (PSA), surface area an

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Mishra, and Snehasis Jana. "Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium." International Journal of Nutrition and Food Science 4, no. 6 (2015): 660–68. https://doi.org/10.5281/zenodo.192200.

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The date palm is mainly cultivated for the production of sweet fruit. Date palm callus initiation medium (DPCIM) is used for plant tissue culture applications. The present work is intended to evaluate the impact of Mr. Trivedi’s biofield energy treatment on physical, thermal and spectral properties of the DPCIM. The control and treated DPCIM were evaluated by various analytical techniques such as X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer (PSA), surface area an

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4

Kumar Trivedi, Mahendra. "Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium." International Journal of Nutrition and Food Sciences 4, no. 6 (2015): 660. http://dx.doi.org/10.11648/j.ijnfs.20150406.20.

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Al-Khayri, Jameel Mohammed, and Poornananda Madhava Naik. "Date palm micropropagation: Advances and applications." Ciência e Agrotecnologia 41, no. 4 (2017): 347–58. http://dx.doi.org/10.1590/1413-70542017414000217.

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ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been

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Othmani, Ahmed, Amel Sellemi, Monia Jemni, Karim Kadri, Leen Leus, and Stefaan P. O. Werbrouck. "In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (Phoenix dactylifera L.)." Horticulturae 10, no. 11 (2024): 1206. http://dx.doi.org/10.3390/horticulturae10111206.

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A stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via the in vitro culture of flower explants. Callus was induced using 2,4-D, followed by shoot regeneration on a medium containing NAA and BAP and rooting with IBA. Regenerated plantlets, confirmed as tetraploid via flow cytometry, were acclimatized and grown for six years. The leaves of tetraploids exhibited significantly wider petiole bases, thicker rachis and spines, broader leaflets, and a more intense green leaf color compared to diploids. However, leaf len

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Panggabean, N. H., M. Basyuni, I. Nurwahyuni, and Elimasni. "Oil palm (Elaeis guineensis Jacq.) micropropagation via somatic embryogenesis from female inflorescences explants." IOP Conference Series: Earth and Environmental Science 912, no. 1 (2021): 012029. http://dx.doi.org/10.1088/1755-1315/912/1/012029.

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Abstract The oil palm (Elaeis guineensis Jacq.) is a perennial woody oil crop in the Arecaceae family. Oil palm is well known for a long regeneration time, therefore, in vitro propagation received great enthusiasm from oil palm industries. Somatic embryogenesis (SE) has become one of the most promising clonal propagation techniques in recent times. This study was aimed to determine a protocol for micropropagation somatic embryogenesis from female inflorescences explants of oil palm. The explant used was obtained from the female inflorescences of the oil palm 2.5 years old Tenera variety. The b

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N. Arafa, Rasha, Sayed A. A. Elsayh, Marwa M. Abdalgaleel, et al. "The genetic stability of date palm shoots regenerated from leaves explant." International Journal of Environment, Agriculture and Biotechnology 8, no. 5 (2023): 101–15. http://dx.doi.org/10.22161/ijeab.85.15.

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The purpose of this research was to develop a micro-propagation method for the date palm Zaghlol cv. using juvenile leaves. To produce the necessary results, different plant growth regulator combinations were used. The leaves were grown on MS medium supplemented with PVP to prevent the explants from browning. The results showed that adding PVP at a concentration of 1.0 g/l considerably reduced browning. On the induction medium, callus formation occurred during the fourth week of culture; however, callus formation (87.5%) was more prevalent on the ¾ MS medium containing with 10.0 mg/l NAA, 1.0

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SHITTU, H. O., G. C. MGBEZE, G. O. OKOLOKO, O. ASEMOTA, and C. R. EKE. "IN VITRO ASSESSMENT OF EMBRYO AGE AND NAPHTHALENE ACETIC ACID SUPPLEMENTATION OF MEDIA IN CALLUS GENERATION OF OIL PALM (Elaeis guineensis Jacq)." Nigerian Journal of Life Sciences (ISSN: 2276-7029) 3, no. 1 (2022): 179–86. http://dx.doi.org/10.52417/njls.v3i1.121.

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Callus generation from embryo explants of the oil palm (Elaeis guineensis Jacq) was observed in relation to age of plants at time of explants excision and types of media used. The two stages of explants were those at 15 and 18 weeks after anthesis. Eeuwens and Murashige & Skoog‘s (MS) media supplemented with Naphthalene acetic acid (NAA) were used. These gave two media variations viz: MS medium supplemented with NAA and Eeuwens‘ medium supplemented with NAA. The best combination for the generation of callus from the embryo explants within 56 days in culture was determined by observing type

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Al-Asadi, Ahmed Z., Aqeel H. Abdulwahid, and Ahmed M. W. Al-Mayahi. "The Effect of Thidiazuron on Callus and in vitro Shoots Development of Date Palm (Phoenix dactylifera L.) cv. Barhee." Basrah Journal of Agricultural Sciences 32 (October 10, 2019): 258–65. http://dx.doi.org/10.37077/25200860.2019.170.

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Date palm (Phoenix dactylifera L.) is one of the most important fruit trees spread in southern Iraq, but because of many circumstances decreased their numbers significantly, the propagation of date palm by tissue culture was the best solution for re-preparation of the problem as it was previously, because propagation is accelerated. Summarizes the working paper in an attempt to find the optimal concentration of thidiazuran (TDZ) (0.0 , 0.5 and 1.0) mgl-1, in production of callus and regeneration of shoots, at different times of initial and callus culturing in medium budding. The results showed

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Mazri, Mouaad Amine, Ilham Belkoura, Reda Meziani, Hajar Es-Saoudy, Fahd Rachad, and Saida Elmaataoui. "Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm." Current Agriculture Research Journal 7, no. 3 (2019): 296–303. http://dx.doi.org/10.12944/carj.7.3.04.

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An efficient somatic embryogenesis system is reported for date palm cv. Al-Fayda, a genotype resistant to the bayoud disease. Callus induction was achieved from adventitious bud explants cultured for 6 months on semi-solid Murashige and Skoog (MS) medium containing 4.5 μM 6-(dimethylallylamino) purine (2iP) and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or picloram. The highest somatic embryogenesis frequency (89%) was obtained on MS medium supplemented with 225 μM 2,4-D. Subsequently, embryogenic cultures were transferred to agitated liquid MS medium (maturation medium)

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Kong, Eveline Y. Y., Julianne Biddle, Sundaravelpandian Kalaipandian, and Steve W. Adkins. "Coconut Callus Initiation for Cell Suspension Culture." Plants 12, no. 4 (2023): 968. http://dx.doi.org/10.3390/plants12040968.

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The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammoniu

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Zainab Tariq Safi Al Ali, Majid Abdulhameed Ibrahim, and Khaun Ali Muhsen. "Impact of Different Concentrations of Aluminum Chloride on Callus Induction and Organogenesis in Date Palm (Phoenix dactylifera L.) cv. "Barhi"." University of Thi-Qar Journal of agricultural research 13, no. 2 (2024): 655–66. https://doi.org/10.54174/2v76bx21.

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The present study was conducted in the Plant Tissue Culture Laboratory, Date Palm Research Center, University of Basrah, Basrah, Iraq for the years 2023 and 2024. The investigation aims to test the effect of adding different concentrations of aluminum chloride (0, 15, 30, and 45mg L-1) to the MS medium prepared to induce callus and indirect shoot regeneration of date palm (Phoenix dactylifera L. cv. Barhi) grown using an in vitro culture technique. The results showed that the aluminum chloride treatment at a concentration of 45mg L-1 significantly outperformed the 15 mg L-1 treatment of alumin

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Emoghene, B. O., M. Idu, C. R. Eke, and O. Asemota. "Effects Of Different Sterilization Regimes & Growth Regulators On Micropropagation Of Female Date Palm (Phoenix dactylifera L.)." Nigerian Journal of Biotechnology 37, no. 1 (2020): 159–68. http://dx.doi.org/10.4314/njb.v37i1.17.

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The success of in vitro culture techniques is always hampered by microbial contamination. The present study was carried out to develop an efficient protocol for date palm explants sterilization for successful somatic embryos induction and plantlets formation of some date palm varieties. The shoot tips were treated with different sterilizing agents at different concentrations and durations of exposure. The use of ethanol (70%), sodium hypochlorite (3.5% & 70%) and mercuric chloride (0.2%) with or without addition of Tween-20 had different effects on decontamination of the date palm explants

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Rekik, Imen, Noureddine Drira, Douglas Grubb, and Amine Elleuch. "Molecular characterization and evolution studies of a SERK like gene transcriptionally induced during somatic embryogenesis in Phoenix Dactylifera L v Deglet Nour." Genetika 47, no. 1 (2015): 323–37. http://dx.doi.org/10.2298/gensr1501323r.

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A somatic embryogenesis receptor kinase like (SERKL) cDNA, designated PhSERKL, was isolated from date palm (Phoenix Dactylifera L) using RACE PCR. PhSERKL protein shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, a transmembrane domain, and kinase domains. Phylogenetic analyses using PHYLIP and Notung 2.7 programs suggest that the SERK proteins of some plant species resulted from relatively ancient duplication events. We predict an ancestor protein of monocots and dicots SERK using FASTML program. Somatic embryogenic c

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16

Al-Mayahi, Ahmed Madi Waheed. "Effect of calcium and boron on growth and development of callus and shoot regeneration of date palm ‘Barhee’." Canadian Journal of Plant Science 100, no. 4 (2020): 357–64. http://dx.doi.org/10.1139/cjps-2019-0084.

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There are some major obstacles in practical applications of date palm tissue culture in the laboratory such as reduced number of shoots, vitrification of tissues, and browning of cultured tissues. The objective of the present study is to determine the effect of boron (B) and calcium (Ca) on enhancing organogenesis of buds from callus, shoot multiplication, and phytochemicals of in vitro cultures of date palm ‘Barhee’. Addition of calcium chloride and boric acid to the medium was most effective on callus for shoot regeneration and number of shoots per jar, where the best result (88.89%, 8.2 sho

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17

Al-Matar, Shatha Matar, Mostafa M. Abo El-Nil, Jameel Al-Khairy, and Gerald Klingaman. "Effect of Sucrose and PEG Concentration on Embryo Maturation and Hyperhydration in Date Palm Suspension Cultures." HortScience 32, no. 3 (1997): 462B—462. http://dx.doi.org/10.21273/hortsci.32.3.462b.

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Cell suspension cultures of four date palm cultivars were established, namely, Niboat Saif, Madjhool, Sukarri, and Berhi. In this study, two factors were tested for their effect on embryo maturation and hyperhydration. The effect of sucrose concentration was assessed by inoculating 0.5 g of embryogenic callus into a liquid MS basal medium supplemented with 10 mg/L inositol, 3 mg/L glycine, 20 mg/L glutamine, and 0, 20, 30, 40, 50 g/L sucrose. Polyethylene glycol (PEG) concentration effect on embryo maturation and hyperhydration was tested. PEG (molecular weight 7000–9000) was added at concentr

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Mu. "Optimization of Coconut Micropropagation via Somatic Embryogenesis." Proceedings 36, no. 1 (2020): 55. http://dx.doi.org/10.3390/proceedings2019036055.

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Coconut (Cocos nucifera L.) is one of the most important tropical palm crops worldwide, but the gap between decreasing productivity and expanding demand is growing and is now becoming a major problem. The global coconut industry is threatened not only by palm senility but by pests/pathogen attack which is becoming exacerbated by climate change. However, conventional breeding methods cannot provide sufficient new planting materials to meet the replacement of senile and damaged palms. Hence, coconut clonal propagation via somatic embryogenesis (SE) is being developed to help meet the demand of t

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MAZRI, Mouaad Amine, Reda MEZIANI, Ilham BELKOURA, Saida ELMAATAOUI, Boutaïna MOKHLESS, and Souad NOUR. "Maturation and Germination of Date Palm (Phoenix dactylifera L.) Somatic Embryos." Notulae Scientia Biologicae 11, no. 1 (2019): 86–93. http://dx.doi.org/10.15835/nsb11110403.

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Maturation and germination of somatic embryos are two crucial steps in the somatic embryogenesis process. Herein, we evaluated the effects of several factors on the maturation and germination of date palm somatic embryos. Globular somatic embryos of cv. ‘Najda’ were cultured on Murashige and Skoog medium at full strength (MS), half strength (1/2MS), and one-third strength (1/3MS), with or without agar, and supplemented with various concentrations of mannitol, sorbitol, polyethylene glycol (PEG, MW 8000) and abscisic acid (ABA). Our results showed that culture medium strength and texture (liqui

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Heidarpoor, Mansoor, Mansoor Kalantar, Mahmoud Khosroshaheli, and Eslam Majidi Hervan. "Investigation on the direct shoot regeneration in date palm CV.Pyarum and possible genetics changes in induced shoots." Nexo Revista Científica 33, no. 02 (2020): 423–30. http://dx.doi.org/10.5377/nexo.v33i02.10781.

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The palm (Phoenix Dactylifera) is one of important trees, and is economically important in south of Iran. Date palm is propagated by the offshoots, number of which is limited. Therefore, adult Date palms produce shoot tips and axillary shoot meristems through the use of a tissue culture. This study was conducted to perform in -vitro tissue culture direct shoot regeneration and determine the best combination of plant regulators and other conditions. To achieve organogenesis and multiplication directly from shoot tips and axillary shoot meristems of Date palm (Phoenix Dactylifera L. Var Pyarum)

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Zein El Din, Amal F. M., Rasmia S. S. Darwesh, Mohamed F. M. Ibrahim, et al. "Antioxidants Application Enhances Regeneration and Conversion of Date Palm (Phoenix dactylifera L.) Somatic Embryos." Plants 11, no. 15 (2022): 2023. http://dx.doi.org/10.3390/plants11152023.

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Many embryogenic systems have been designed to generate somatic embryos (SEs) with the morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation, several antioxidants were added to the maturation media of the developing somatic embryos of date palm. Explant material was a friable embryogenic callus that was placed in maturation media containing ABA at 0.5 mg L−1, 5 g L−1 polyethylene glycol, and 10 g L−1 phytagel. Furthermore, α-tocopherol or reduced glutathione (GSH) were used separately at (25 and 50 mg L−1). These treatments were compared to a

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Al-Asadi, Ahmed Z. R., Ahmed M. W. Al-Mayahi, and Khairullah M. Awad. "Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee." Folia Oecologica 51, no. 1 (2024): 56–65. http://dx.doi.org/10.2478/foecol-2024-0006.

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Abstract The investigation was carried out to evaluate the influence of the dicamba (3,6-Dichloro-2-methoxybenzoic acid) (DIC) and casein hydrolysate (CH) on the callus growth, shoot multiplication, and some biochemical constituents of date palm cv. Barhee cultured in vitro. Both DIC and CH were required for callus growth and shoots regeneration. The medium supplemented with 4.0 mg l−1 DIC in combination with 1.0 g l−1 CH gave the highest callus weight (287 mg), while the maximum response rate and the number of shoots per jar (86.67% and 15.07 shoots/jar) were found in MS media equipped with 4

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Naik, Poornananda Madhava, and Jameel Mohammed Al-Khayri. "Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)." Ciência e Agrotecnologia 42, no. 5 (2018): 464–73. http://dx.doi.org/10.1590/1413-70542018425021118.

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ABSTRACT Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry w

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Ribeiro, Juliana Martins, Silvio Lopes Teixeira, Joselita Cardoso de Souza, et al. "In vitro response of date palm (Phoenix dactylifera L.) inflorescence explants to high 2iP and 2,4-D concentrations." MAY 2020, no. 14(05):2020 (May 20, 2020): 831–35. http://dx.doi.org/10.21475/ajcs.20.14.05.p2317.

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One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, e

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DeMason, Darleen A., Daniel Widney, and James I. Stillman. "In vitro and transplantation experiments with germination of date embryos." Canadian Journal of Botany 70, no. 5 (1992): 965–74. http://dx.doi.org/10.1139/b92-121.

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In vitro experiments with date palm embryos were designed to determine (i) which carbon sources support growth and (ii) what culture conditions promote haustorial growth and development. Date palm embryos were also transplanted into Washington filifera seeds to deterine whether any developmental characteristics were determined by the seed or endosperm. Date embryos did not grow on media containing starch or galactomannan any better than they do on medium lacking a carbon source. Good growth occurred on medium containing mannose, glucose, or sucrose as the carbon source. Embryos imbibed in the

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Daayf, F., M. El Bellaj, M. El Hassni, F. J'Aiti, and I. El Hadrami. "Elicitation of soluble phenolics in date palm (Phoenix dactylifera) callus by Fusarium oxysporum f. sp. albedinis culture medium." Environmental and Experimental Botany 49, no. 1 (2003): 41–47. http://dx.doi.org/10.1016/s0098-8472(02)00048-5.

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Hassan, L. "Successful genetic transformation in date palm (Phoenix dactylifera)." Journal of the Bangladesh Agricultural University 11, no. 2 (2014): 171–76. http://dx.doi.org/10.3329/jbau.v11i2.19841.

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The introduction of foreign genes into most of the Phoenix spp using recombinant DNA technology is not a straight forward task. In Phoenix spp application of this technology towards successful transformation proved to be a more difficult one so far no report on the successful regeneration of transgenic date palm plants has been published. We developed an efficient and reproducible variety-independent method for producing transgenic date palm (Phoenix spp) via Agrobacterium-mediated transformation. Agrobacterium rhizogenes strains LBA 9402 were used and for cotransformation experiments the st

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Al-Mayahi, Ahmed Madi Waheed, and Abdulminam Hussian Ali. "Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv." Folia Oecologica 48, no. 1 (2021): 110–17. http://dx.doi.org/10.2478/foecol-2021-0012.

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Abstract Some obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis and multiplication efficiency, and frequent tissue vitrification. This investigation studied the effect of five types of gelling agents (Danish Agar, Cero Agar Type 8952, Chile Agar, Gerlite Food Grade, and Agar-Agar.) on in vitro regeneration and bud multiplication of Phoenix dactylifera L. cv. Showathy. The results showed that the highest percentages of callus producing buds and average bud formation (77.78%, 11.5 buds, and 72.2

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Dlaigan, Yousef I., A. E. Said, and M. A. El-Hamady. "Establishment of Excised Date Palm (Phoenix dactylifera L.) Roots in Vitro." HortScience 30, no. 4 (1995): 875D—875. http://dx.doi.org/10.21273/hortsci.30.4.875d.

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Several trials were conducted with the objective of obtaining an explant for the establishment of date palm root culture in vitro. These trials included disinfecting and germinating seeds of three cultivars on several autoclaved culture media, the influence of incubation temperature on different germination parameters, and the quality of roots before excision and after culture in nutrient media. Three culture media were used: distilled water only; minimal organics that consisted of MS salts, 3% sucrose, modified White's organics, 0.01% inositol, and 0.15% activated charcoal; and 1/2 MS salts m

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Mahendra, Trivedi, Branton Alice, Trivedi Dahryn, and Nayak Gopal. "Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium." Science Publishing Group, November 9, 2015. https://doi.org/10.5281/zenodo.813643.

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The date palm is mainly cultivated for the production of sweet fruit. Date palm callus initiation medium (DPCIM) is used for plant tissue culture applications. The present work is intended to evaluate the impact of Mr. Trivedi's biofield energy treatment on physical, thermal and spectral properties of the DPCIM. The control and treated DPCIM were evaluated by various analytical techniques such as X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer (PSA), surface area analyzer

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Abd Elaziem, Tamer Mahfouz, Manal El-salato Ala El-naby Ahmed, and Gamil Rayan Abou El-Dis. "In vitro propagation for conservation of the rare date palm (Phoenix dactylifera L.) ‘Amri’ using immature inflorescence." In Vitro Cellular & Developmental Biology - Plant, September 28, 2022. http://dx.doi.org/10.1007/s11627-022-10296-3.

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AbstractImmature female inflorescence plays a significant role in date palm micropropagation because inflorescences are available with no practical limit as the source of explants. Moreover, using floral buds for propagation helps in the conservation of date palm biodiversity and the enhancement of socioeconomically valuable landraces. With the goal of avoiding undesirable genetic variability, the optimal combinations and concentrations of plant growth regulators and other medium compositions were investigated to achieve direct organogenesis and multiplication from the immature female inflores

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SAPTARI, Rizka Tamania, and SUMARYONO. "Embriogenesis somatik dari pucuk tunas tanaman kurma (Phoenix dactylifera L.) Somatic embryogenesis from shoot tip of date palm (Phoenix dactylifera L.))." E-Journal Menara Perkebunan 86, no. 2 (2018). http://dx.doi.org/10.22302/iribb.jur.mp.v86i2.313.

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Date palm (Phoenix dactylifera L.) is the most important crop in the dry areas of the Middle East and North Africa. This palm has been introduced to many countries but has not been grown commercially in Indonesia. Date palm propaga-tion by seeds is easy but its progenies are varied and a half of them are male trees that will not produce fruits. Meanwhile, the propagation by offshoots is impractical and technically difficult. Tissue culture makes it possible to massproduce of genetically identicalsuperior date palms. This research aimed to develop somatic embryogenesis (SE) of date palm using s

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Shehata, Wael. "ENHANCING ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENT IN DATE PALM (Phoenix dactylifera L.) CALLUS CULTURES THROUGH TRACE ELEMENT SUPPLEMENTATION." Agrociencia, May 26, 2025, 1–18. https://doi.org/10.47163/agrociencia.v59i4.3405.

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This study investigates the impact of trace element supplementation on phenolic content, antioxidant activity, and callus initiation in Hassawi date palm (Phoenix dactylifera L.) cultivars (Khalas, Ruziz, and Shishi) grown on Murashige and Skoog (MS) medium. Varying concentrations of trace elements (MgSO4·7H2O, MnSO4·4H2O, ZnSO4·7H2O, and CuSO4·5H2O at zero, half, full, and double Stock E) were tested to evaluate their effects on secondary metabolite production and oxidative stress management. Results demonstrated that double concentrations of Stock E significantly enhanced phenolic content (2

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Al-Mayahi, Ahmed Madi Waheed. "Stimulating Effect of Cefotaxime and Silver Nanoparticles on Plant Regeneration in Date Palm Tissue Culture." Journal of Horticultural Research, December 25, 2024. https://doi.org/10.2478/johr-2024-0018.

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Abstract The essential challenges in practical applications of date palm micropropagation include explant browning, reduced callus growth, low multiplication rate, and frequent tissue contamination. Our study aimed to evaluate the effects of cefotaxime (Cefo) and silver nanoparticles (AgNPs) on microbial contamination removal, callus growth, and shoot regeneration in date palm micropropagation. Cultures were initiated from bud tips of ‘Barhee’ date palm. Murashige and Skoog medium with auxins, cytokinins, and activated charcoal was amended with Cefo (50, 100, and 200 mg·l−1), AgNPs (0.125 and

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Sinta, Masna Maya, Rizka Tamania Saptari, Imron Riyadi, and Sumaryono Sumaryono. "Callus induction and regeneration of date palm (Phoenix dactylifera L.) cv. Zambli through somatic embryogenesis from four layers of young leaves explant." E-Journal Menara Perkebunan 92, no. 2 (2024). http://dx.doi.org/10.22302/iribb.jur.mp.v92i2.588.

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The Zambli variety of date palm shows potential for cultivation in tropical regions, as its fruits are edible during the Rutab stage. However, large-scale production of Zambli seedlings presents a significant challenge. In vitro propagation offers a solution for producing large quantities of clonal planting material. This study focuses on inducing callus formation from the four-layered shoot tips of young leaves and regenerating these calli into plantlets through somatic embryogenesis. Explants were cultured on a modified MS medium with 10, 50, or 100 mg L-1 2,4-dichlorophenoxyacetic acid (2,4

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Al-Mayahi, Ahmed Madi Waheed. "Effect of Ancymidol and Phloroglucinol on the Number and the Quality of Shoots in the Micropropagation of Date Palm (Phoenix dactylifera L.)." Journal of Horticultural Research, September 28, 2024. http://dx.doi.org/10.2478/johr-2024-0013.

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Abstract The disadvantages of practical date palm micropropagation are tissue browning, low callus proliferation rate, low multiplication efficiency, and vitrification. The aim of the study was to determine the effect of ancymidol (Ancy) and phloroglucinol (PG) on the growth and some biochemical components of the ‘Barhee’ date palm cultured in vitro. The combination of 0.75 mg·l−1 Ancy and 50 mg·l−1 PG was found to be the most effective in terms of callus regeneration rate (89%) and number of shoots (14.3). A reduction in browning was observed in tissues cultured on media supplemented with 0.7

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Zayed, Eman M. M., S. S. Darwesh Rasmia, and Khaled Abd-El Hamed. "Plant Regeneration from Mature Inflorescence of Date Palm by Using Moringa Extract." Asian Research Journal of Agriculture, January 6, 2020, 1–11. http://dx.doi.org/10.9734/arja/2020/v12i130072.

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Plant tissue culture is becoming an important technique for the massive propagation of date palm and the available source lead to the success of this process. The mature female inflorescence is a novel and available source for in vitro propagation of date palm, nowadays become needed at the large scale to usage of organic nature materials in agriculture practice which are rich in plant growth regulators as well as micro elements. This study describes in vitro plant regeneration from mature female inflorescence explants of evaluating date palm (Phoenix dactylifera L.) by using the methanol extr

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Abdalla, Mohanned Abdalla Ali, and Abdel Gaffer Elhag Said. "Acceleration of Callus Formation in Date Palm (Phoenix dactylifera L.) Zygotic Embryos." University of Khartoum Journal of Agricultural Sciences 25, no. 1 (2023). http://dx.doi.org/10.53332/uofkjas.v25i1.1789.

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This study was carried out to test the effect of water soaking ofseeds of date palm ( P. dactylifera L.) as a pre-empryo excision treatment,on in vitro culture of zygotic empryos. The objective was optimization oforganogenic callus formation. Mature seeds of “Barakawi” and “MishrigWad Laggi” date palm cultivars were used as sources of embryos.Durations of 0- up to 9-days of seed water soaking, prior to embryoexcision and culture, were tested. Murashige and Skoog (1962) mediumwas used as basal medium. The cultures responded positively to the watersoaking treatments and resulted in the organogen

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Shahrour, W. G., M. A. Shatnawi, R. A. Shibli, et al. "Callus induction, shoot regeneration, and conservation of in vitro grown date palm (Phoenix dactylifera)." Brazilian Journal of Biology 84 (2024). http://dx.doi.org/10.1590/1519-6984.284231.

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Abstract Date palm (Phoenix dactylifera( cv. Medjool is a significant plant, grown in Jordan. In vitro propagation gives operative resources for the significant propagation of date palms. Maximum callus induction was achieved from MS media supplemented with benzyl amino purine (BA) and naphthalene acetic acid (NAA). The highest plant regeneration was recorded on MS medium supplemented with dichlorophenoxyacetic acid (2,4-D) at 3.0 mg/L, and BA at 2.0 mg/L. A significant positive impact on shoot formation was recorded on MS medium supplemented with 1.0 mg/L BA with 0.5 to 1.5 mg/L NAA in both l

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Al-Mayahi, Ahmed Madi Waheed. "Effect of Sodium Nitroprusside with Plant Growth Regulators on in vitro Propagation and Genetic Stability of ‘Barhee’ Date Palm (Phoenix dactylifera L.)." Journal of Horticultural Research, April 8, 2025. https://doi.org/10.2478/johr-2025-0003.

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Abstract Date palm micropropagation still faces many limitations in practical applications due to tissue browning, reduced callus growth and development, low organogenesis and multiplication efficiency, and low rooting rate. This study investigated the effects of sodium nitroprusside (SNP), a source of nitric oxide and plant growth regulators, on the growth attributes and some biochemical constituents of in vitro cultured ‘Barhee’ date palm. The addition of SNP to the culture media was more effective than the individual application of growth regulators regarding callus growth, shoot regenerati

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Khaskheli, Allah Jurio, and Shahla Baloch. "Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.)." Kashmir Journal of Science 4, no. 01 (2025). https://doi.org/10.63147/krjs.v4i01.128.

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The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA and Kinetin. The interpretations on the existence time interval, days reserved to start shoot initiation, total shoots number and shoots length were noted. Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) ex

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Khaskheli, Allah Jurio, and Shahla Baloch. "Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.)." Kashmir Journal of Science 4, no. 01 (2025). https://doi.org/10.63147/39y1tk23.

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The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA and Kinetin. The interpretations on the existence time interval, days reserved to start shoot initiation, total shoots number and shoots length were noted. Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) ex

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Al-Mayahi, Ahmed Madi Waheed. "In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources." Journal of Genetic Engineering and Biotechnology 19, no. 1 (2021). http://dx.doi.org/10.1186/s43141-021-00177-4.

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Abstract Background Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. Results The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe), compared with other treat

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Hassan, Mona M., Mai A. Allam, I. M. Shams El Din, Mervat H. Malhat, and Rania A. Taha. "High-frequency direct somatic embryogenesis and plantlet regeneration from date palm immature inflorescences using picloram." Journal of Genetic Engineering and Biotechnology 19, no. 1 (2021). http://dx.doi.org/10.1186/s43141-021-00129-y.

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Abstract Background Date palm (Phoenix dactylifera L.) is a traditional crop in arid and semi-arid areas. Its vegetative propagation can be achieved by offshoots, but possible number of offshoots in mother palm trees is limited. Micropropagation is a highly recommended strategy for obtaining date palm elite cultivars using shoot tip and immature inflorescences. In this study, micropropagation procedure using inflorescence explants of Medjool cv. is described. For culture initiation, explants from different spathe lengths were cultivated on Murashige and Skoog medium (MS) supplemented with picl

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SUMARYONO, Imron RIYADI, Pauline D. KAS, and Gale GINTING. "Pertumbuhan dan perkembangan kalus embriogenik dan embrio somatik kelapa sawit (Elaeis guineensis Jacq.) pada sistem perendaman sesaat Growth and differentiation of embryogenic callus and somatic embryos of oil palm (Elaeis guineensis Jacq.) in a temporary immersion system." E-Journal Menara Perkebunan 75, no. 1 (2016). http://dx.doi.org/10.22302/iribb.jur.mp.v75i1.152.

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SummaryIn temporary immersion system (TIS),plant materials are exposed to the medium fora short time, therefore they are more exposedto the air and a lack of oxygen frequentlyexperienced by a liquid culture can be avoided.This experiment was conducted to determinethe procedure for callus proliferation up tosomatic embryo germination of oil palm(Elaeis guineensis Jacq.) in TIS culture.Embryogenic calli of oil palm clone MK 638from Marihat Research Institute were culturedon solid medium in the dark culture room andthen used as materials for TIS. Immersion timefor all cultures was three minutes e

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SUMARYONO, Imron RIYADI, Pauline D. KAS, and Gale GINTING. "Pertumbuhan dan perkembangan kalus embriogenik dan embrio somatik kelapa sawit (Elaeis guineensis Jacq.) pada sistem perendaman sesaat Growth and differentiation of embryogenic callus and somatic embryos of oil palm (Elaeis guineensis Jacq.) in a temporary immersion system." E-Journal Menara Perkebunan 75, no. 1 (2016). http://dx.doi.org/10.22302/ppbbi.jur.mp.v75i1.152.

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SummaryIn temporary immersion system (TIS),plant materials are exposed to the medium fora short time, therefore they are more exposedto the air and a lack of oxygen frequentlyexperienced by a liquid culture can be avoided.This experiment was conducted to determinethe procedure for callus proliferation up tosomatic embryo germination of oil palm(Elaeis guineensis Jacq.) in TIS culture.Embryogenic calli of oil palm clone MK 638from Marihat Research Institute were culturedon solid medium in the dark culture room andthen used as materials for TIS. Immersion timefor all cultures was three minutes e

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Jassim, Naji Salim. "Effect of Salicylic Acid in Inhibiting Fungal Contamination in in vitro Cultures of Date Palm (Phoenix dactylifera L.) and Enhancing Embryogenesis and Plantlet Development." Journal of Horticultural Research, December 25, 2024. https://doi.org/10.2478/johr-2024-0019.

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Abstract In this study, we evaluated the role of salicylic acid in diminishing fungal contamination in in vitro cultures of date palm and the effect of salicylic acid on the regeneration of somatic embryos from callus formed on shoot tips. The most prevalent fungi were Alternaria alternata (37%), Fusarium solani (25%), Aspergillus fumigatus (18%), and Penicillium expansum (6%). Salicylic acid limited and at higher concentrations retarded mycelial growth using potato dextrose agar. Salicylic acid at concentrations of 1.5 and 2.0 mM added to MS medium with 2iP and NAA significantly increased the

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