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Journal articles on the topic 'Dead Staining'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Volf, D., and R. Jurecic. "Cell staining with DAPI: or dead?" Trends in Genetics 5 (1989): 292. http://dx.doi.org/10.1016/0168-9525(89)90107-8.

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2

Liu, Yuting, Yuanhong Xu, and Qin Wen. "Carbon dots for staining bacterial dead cells and distinguishing dead/alive bacteria." Analytical Biochemistry 687 (April 2024): 115432. http://dx.doi.org/10.1016/j.ab.2023.115432.

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3

Li, Yan-Hong, Jia Zeng, Zihao Wang, et al. "Sulfur-Doped Organosilica Nanodots as a Universal Sensor for Ultrafast Live/Dead Cell Discrimination." Biosensors 12, no. 11 (2022): 1000. http://dx.doi.org/10.3390/bios12111000.

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Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much large

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4

Maibach, Alain, and Pierre Goeldlin de Tiefenau. "Staining Technique for the Integument of Dead and Living Aquatic Larvae (Diptera: Syrphidae)." Entomologia Generalis 17, no. 1 (1992): 69–71. http://dx.doi.org/10.1127/entom.gen/17/1992/69.

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5

Hiraoka, Yoshinori, and Kazuhide Kimbara. "Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry." Applied and Environmental Microbiology 68, no. 4 (2002): 2031–35. http://dx.doi.org/10.1128/aem.68.4.2031-2035.2002.

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ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and

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6

PENG, YING-SHIN, SARAH J. LOCKE, MEDHAT E. NASR, T. P. LIU, and MARY ANN MONTAGUE. "Differential staining for live and dead sperm of honey bees." Physiological Entomology 15, no. 2 (1990): 211–17. http://dx.doi.org/10.1111/j.1365-3032.1990.tb00509.x.

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7

Brenan, M., and C. Parish. "Simultaneous determination of viable and dead lymphocytes by fluorescent staining." Journal of Immunological Methods 102, no. 1 (1987): 147. http://dx.doi.org/10.1016/s0022-1759(87)80020-0.

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8

Forson, Tetteh-Quarcoo, Ahenkorah, et al. "Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs." Medical Sciences 7, no. 4 (2019): 64. http://dx.doi.org/10.3390/medsci7040064.

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This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S.

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9

Ciancio, G., A. Pollack, M. A. Taupier, N. L. Block, and G. L. Irvin. "Measurement of cell-cycle phase-specific cell death using Hoechst 33342 and propidium iodide: preservation by ethanol fixation." Journal of Histochemistry & Cytochemistry 36, no. 9 (1988): 1147–52. http://dx.doi.org/10.1177/36.9.2457047.

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We developed a rapid technique for preservation of Hoechst 33342/propidium iodide-stained cells, using ethanol as a fixative. Combined staining with these dyes makes possible analysis of cell-cycle phase-specific cell death. The technique relies on exclusion of propidium iodide from the viable cells, whereas Hoechst stains all of the cells. The bivariate histograms resulting from the flow cytometric analysis contain the equivalent of two single-parameter DNA histograms, one of the living and the other of the dead cell population. Preservation of staining involved addition of 25% ethanol in PBS

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10

Pasini, Erica M., Denise van den Ierssel, Henri J. Vial, and Clemens HM Kocken. "A novel live-dead staining methodology to study malaria parasite viability." Malaria Journal 12, no. 1 (2013): 190. http://dx.doi.org/10.1186/1475-2875-12-190.

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11

Medema, G. J., F. M. Schets, H. Ketelaars, and G. Boschman. "Improved detection and vital staining of Cryptosporidium and Giardia with flow cytometry." Water Science and Technology 38, no. 12 (1998): 61–65. http://dx.doi.org/10.2166/wst.1998.0501.

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Flow cytometry with fluorescence activated cell sorting (FACS) was used to purify Cryptosporidium (oo)cysts and Giardia cysts from water. With this purification step Cryptosporidium and Giardia were found in a higher percentage of the samples and significantly higher Giardia concentrations were detected in these positive samples. Because FACS removed most of the debris from the concentrated water sample, the microscopic preparations could be examined more rapidly for the presence of (oo)cysts and the morphological characteristics of the (oo)cysts could be interpreted more unambiguously than wi

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12

Petrunkina, A. M., R. A. P. Harrison, and E. Töpfer-Petersen. "Only low levels of spermadhesin AWN are detectable on the surface of live ejaculated boar spermatozoa." Reproduction, Fertility and Development 12, no. 8 (2000): 361. http://dx.doi.org/10.1071/rd00117.

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The zona-binding protein family of spermadhesins are constituents of boar seminal plasma that are generally believed to attach to the acrosomal region of spermatozoa and thereby assist sperm interaction with the zona pellucida at fertilization. However, previous studies have yielded conflicting results with respect to amounts of adhesin bound to ejaculated cells, to the distribution of bound adhesin within the sperm population, and the regionalization of binding on the sperm surface. In the present study, spermadhesin AWN in unfixed living suspensions of boar spermatozoa was assessed by means

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13

Révay, T., S. Nagy, A. Kovács, et al. "Head area measurements of dead, live, X- and Y-bearing bovine spermatozoa." Reproduction, Fertility and Development 16, no. 7 (2004): 681. http://dx.doi.org/10.1071/rd04013.

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The head area of bull spermatozoa was measured after viability and acrosome staining using trypan blue and Giemsa stains, followed by X- and Y-chromosome-specific fluorescence in situ hybridisation (FISH). The former staining made possible the categorisation of cells according to morphology and membrane integrity, whereas the latter allowed distinction of spermatozoa bearing X- and Y-chromosomes. Individual spermatozoa could be followed during the consecutive steps of staining, measurement and FISH. Using a high-resolution digital imaging system and measurement software, the head area of more

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14

Krapoth, Tim Christopher, Gina Sophie Henle, Mihrije Avdyli, et al. "Wanted: Dead or Alive Cells with Propidium Iodide Staining in Liver Tissue." International Journal of Molecular Sciences 25, no. 24 (2024): 13521. https://doi.org/10.3390/ijms252413521.

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This study demonstrates the effectiveness of propidium iodide as a reliable marker for detecting dead or dying cells in frozen liver tissue sections. By comparing propidium iodide staining with the widely used Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, both methods showed consistent results in disease models such as alcohol-induced fibrosis and Western diet-induced fatty liver. Additionally, propidium iodide was successfully co-stained with other fluorescent markers, like phalloidin (for actin filaments) and antibodies targeting collagen, enabling detailed spat

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Наволокин, Н. А., Н. В. Полуконова, Д. А. Мудрак та ін. "Преимущества и возможности флуоресцентных методов для визуализации апоптоза и аутофагии в опухолевых клетках человека in vitro-=SUP=-*-=/SUP=-". Журнал технической физики 126, № 6 (2019): 771. http://dx.doi.org/10.21883/os.2019.06.47772.52-19.

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The possibilities of using fluorescent research methods and their advantages for visualization and determination of the type of programmed cell death of human tumor cells under the action of flavonoids in experiments in vitro were investigated. The object of the study were the tumor cells of cervical cancer HeLa and A498 kidney carcinoma, the flavonoid containing extract of hedge hyssop (Gratiola officinalis L.) was used for testing in the experiments. The following fluorescent methods were used: the "living and dead" test with double staining - - iodide propidium and acridine orange, the meth

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16

CASEY, PATRICK J., ROBERT B. HILLMAN, KATHRYN R. ROBERTSON, ASHLEY I. YUDIN, IRWIN K. M. LIU, and ERMA Z. DROBNIS. "Validation of an Acrosomal Stain for Equine Sperm that Differentiates between Living and Dead Sperm." Journal of Andrology 14, no. 4 (1993): 289–97. http://dx.doi.org/10.1002/j.1939-4640.1993.tb03367.x.

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ABSTRACT: An acrosomal staining technique that can differentiate between living and dead sperm was developed for equine sperm. The fluoresceinated lectin Pisum sativum agglutinin (FITC‐PSA) was used to identify the presence or absence of acrosomal contents, while the supravital nuclear dye Hoechst 33258 (H258) was used to assess viability. The accuracy of the FITC‐PSA acrosomal stain was tested by comparing the percentage of sperm that had lost their acrosomal contents, detected by the staining method, with that detected by transmission electron microscopy (TEM). Following capacitation in vitr

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17

Seepersad, Balram, Kelvin Ramnath, Shyam Dyal, and Reeza Mohammed. "The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours… A Re-Evaluation of the US Environmental Protection Agency Methodology." Journal of Energy Resources Technology 126, no. 3 (2004): 215–18. http://dx.doi.org/10.1115/1.1667532.

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There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will fac

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18

Jejurkar, Valmik P., Gauravi Yashwantrao, Atharva Suryavanshi, et al. "Rationally designed Tröger's base decorated bis-carbazoles as twisted solid-state emitting materials and dead bacterial cell imaging." New Journal of Chemistry 46, no. 12 (2022): 5730–40. http://dx.doi.org/10.1039/d1nj05140g.

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19

Vitipon, Marianne, Esther Akingbagbohun, and Thierry Rabilloud. "The VVBlue assay: a plate-readable, dye exclusion-based cell viability assay for the toxicological testing of chemicals." RSC Advances 15, no. 23 (2025): 17885–96. https://doi.org/10.1039/d4ra08606f.

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20

Berney, Michael, Frederik Hammes, Franziska Bosshard, Hans-Ulrich Weilenmann, and Thomas Egli. "Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry." Applied and Environmental Microbiology 73, no. 10 (2007): 3283–90. http://dx.doi.org/10.1128/aem.02750-06.

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ABSTRACT The commercially available LIVE/DEAD BacLight kit is enjoying increased popularity among researchers in various fields of microbiology. Its use in combination with flow cytometry brought up new questions about how to interpret LIVE/DEAD staining results. Intermediate states, normally difficult to detect with epifluorescence microscopy, are a common phenomenon when the assay is used in flow cytometry and still lack rationale. It is shown here that the application of propidium iodide in combination with a green fluorescent total nucleic acid stain on UVA-irradiated cells of Escherichia

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21

Su, Mon Aung, Kanokwiroon Kanyanatt, Phairatana Tonghathai, and Chatpun Surapong. "Live and dead cells counting from microscopic trypan blue staining images using thresholding and morphological operation techniques." International Journal of Electrical and Computer Engineering (IJECE) 9, no. 4 (2019): 2460–68. https://doi.org/10.11591/ijece.v9i4.pp2460-2468.

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Cell counting is a required procedure in biomedical experiments and drug testing. Manual cell counting performed with a hemocytometer is time consuming and individual dependence. This study reported the development of a computer-assisted program for trypan blue stained-cell counting using digital image analysis. Images of trypan blue-stained breast cancer cells line were obtained by a microscope with a digital camera. Undesired noise and debris were removed by applying a guided image filter. Color space HSV (Hue, Saturation and Value) conversion and grayscale conversion were performed for dist

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22

Auty, M. A. E., G. E. Gardiner, S. J. McBrearty, et al. "Direct In Situ Viability Assessment of Bacteria in Probiotic Dairy Products Using Viability Staining in Conjunction with Confocal Scanning Laser Microscopy." Applied and Environmental Microbiology 67, no. 1 (2001): 420–25. http://dx.doi.org/10.1128/aem.67.1.420-425.2001.

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ABSTRACT The viability of the human probiotic strains Lactobacillus paracasei NFBC 338 and Bifidobacterium sp. strain UCC 35612 in reconstituted skim milk was assessed by confocal scanning laser microscopy using the LIVE/DEAD BacLight viability stain. The technique was rapid (<30 min) and clearly differentiated live from heat-killed bacteria. The microscopic enumeration of various proportions of viable to heat-killed bacteria was then compared with conventional plating on nutrient agar. Direct microscopic enumeration of bacteria indicated that plate counting led to an underestimation of bac

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23

Elliott, David T., and Kam W. Tang. "Simple staining method for differentiating live and dead marine zooplankton in field samples." Limnology and Oceanography: Methods 7, no. 8 (2009): 585–94. http://dx.doi.org/10.4319/lom.2009.7.585.

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24

Lawn, S. D., and M. P. Nicol. "Editorial Commentary: Dead or Alive: Can Viability Staining Predict Response to Tuberculosis Treatment?" Clinical Infectious Diseases 60, no. 8 (2014): 1196–98. http://dx.doi.org/10.1093/cid/ciu1156.

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25

Kirchhoff, Christian, and Heribert Cypionka. "Propidium ion enters viable cells with high membrane potential during live-dead staining." Journal of Microbiological Methods 142 (November 2017): 79–82. http://dx.doi.org/10.1016/j.mimet.2017.09.011.

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26

Martín-Partido, G., I. S. Alvarez, L. Rodríguez-Gallardo, and J. Navascués. "Differential staining of dead and dying embryonic cells with a simple new technique." Journal of Microscopy 142, no. 1 (1986): 101–6. http://dx.doi.org/10.1111/j.1365-2818.1986.tb02742.x.

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27

Shetty, Archana, and Sandhyarani Kanna. "Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures." Indian Journal of Obstetrics and Gynecology Research 10, no. 2 (2023): 227–29. http://dx.doi.org/10.18231/j.ijogr.2023.048.

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Eosin Nigrosin staining for assessment of sperm vitality is an essential component of basic semen analysis as it helps differentiate between dead and immotile sperms, and has clinical implications in terms of patient treatment and follow up. This staining technique involves minimal use of reagents and simple procedural steps. Standardization of the same is pertinent to warrant accurate and reproducible results in medical laboratories, even those not specialized in infertility care. We wish to share our hands on experience through various stages in implementing this staining technique from chal

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Kahlisch, L., K. Henne, L. Groebe, J. Draheim, M. G. Höfle, and I. Brettar. "Molecular analysis of the bacterial drinking water community with respect to live/dead status." Water Science and Technology 61, no. 1 (2010): 9–14. http://dx.doi.org/10.2166/wst.2010.773.

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The assessment of the physiological state of the bacteria in drinking water is a critical issue, especially with respect to the presence of pathogenic bacteria. Though molecular methods can provide insight into the taxonomic composition of the drinking water microflora, the question if a bacterial species is alive or dead still needs to be addressed. To distinguish live and dead bacteria at the taxonomic level, we combined three methods: i) a staining procedure indicating membrane-injured cells (using SYTO9 and Propidium Iodide) that is considered to distinguish between live and dead cells, ii

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Yamashita, Kyohei, Koji Yamada, Kengo Suzuki, and Eiji Tokunaga. "Noninvasive and safe cell viability assay forEuglena gracilisusing natural food pigment." PeerJ 7 (April 4, 2019): e6636. http://dx.doi.org/10.7717/peerj.6636.

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Noninvasive and safe cell viability assay is required in many fields such as regenerative medicine, genetic engineering, single-cell analysis, and microbial food culture. In this case, a safe and inexpensive method which is a small load on cells and the environment is preferable without requiring expensive and space-consuming equipment and a technician to operate. We examined eight typical natural food pigments to findMonascuspigment (MP) or anthocyanin pigment (AP) works as a good viability indicator of dye exclusion test (DET) forEuglena graciliswhich is an edible photosynthetic green microa

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30

Hellmold, H., D. Teuteberg, J. Tetens, and C. Blaschka. "83 Validation of propidium iodide dye for live-dead staining of bovine blastocysts: Preliminary results." Reproduction, Fertility and Development 32, no. 2 (2020): 168. http://dx.doi.org/10.1071/rdv32n2ab83.

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A widely used criterion routine laboratories utilise for the selection and classification of oocytes and embryos is morphological appearance. In order to make an objective validation of produced embryos, it is necessary to have a method that can help to distinguish viable cells. One simple and easy-to-perform method is the live-dead staining protocol. Usually, ethidium homodimer (EthD) is used to stain the dead cells, in conjunction with Hoechst 33342 for total cell numbers (Stinshoff et al. 2011). However, EthD is a harmful, toxic, and expensive staining dye. Therefore, the aim of this study

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Ravindran, Vivek B., Esmaeil Shahsavari, Sarvesh K. Soni, and Andrew S. Ball. "Viability determination of Ascaris ova in raw wastewater: a comparative evaluation of culture-based, BacLight Live/Dead staining and PMA-qPCR methods." Water Science and Technology 80, no. 5 (2019): 817–26. http://dx.doi.org/10.2166/wst.2019.286.

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Abstract Accurate evaluation of viable Ascaris ova in wastewater is the key to mitigating Ascaris reinfections in endemic regions. In this study, the viability of Ascaris ova in raw wastewater was determined using three different detection methods: culture-based, BacLight Live/Dead staining and propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR). Furthermore, comparative assessment of viability utilising the aforementioned detection methods was performed using seeded experiments in wastewater. The percentage of viability was: culture-based (82%), BacLight Live/Dead staining (

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32

Kılıç, Tuğba, Ebru Sinanoğlu, Emine Kırbay, Soner Kazaz, and Sezai Ercişli. "Determining appropriate methods for estimating pollen viability and germination rates in lisianthus." Acta Scientiarum Polonorum Hortorum Cultus 23, no. 3 (2024): 33–42. http://dx.doi.org/10.24326/asphc.2024.5378.

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Crossbreeding is a multi-stage process with inherent challenges and risks in developing new varieties. Success hinges on selecting highly fertile parents. In species like lisianthus, uncertainty persists regarding the optimal methods for assessing pollen quality, which is crucial for evaluating pollen parent fertility. This study seeks to identify the most reliable techniques for this purpose. Fresh and dead pollen from four lisianthus (Eustoma grandiflorum) varieties was used. The dead pollen was obtained by thermal inactivation. Five chemical staining methods (iodine-potassium iodide, 2,3,5-

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33

Truong, Minh-Dung, Thanh-Tam Nguyen-Thi, Thanh-Tan Nguyen-Ngoc, et al. "Decellularized Membrane Derived from the Cell-Produced Extracellular Matrix of 1-Day-Old Porcine Cartilage Can Be a Substitute for Periosteal Patches in Autologous Chondrocyte Implantation." Applied Sciences 15, no. 4 (2025): 2237. https://doi.org/10.3390/app15042237.

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(1) Autologous chondrocyte implantation (ACI) is a prominent method for treating cartilage damage, but periosteal patches can cause chondrocyte leakage. This study evaluates the potential of a decellularized membrane derived from the cell-produced extracellular matrix of 1-day-old porcine cartilage (pcECM-DM) to act as a substitute for periosteal patches. (2) The interaction between young rabbit chondrocyte cells and pcECM-DM was assessed through cytotoxicity, differentiation, cell viability, cell migration, and adhesive ability. Rabbit chondrocyte cells, cultivated until passage two, were see

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Sarvel, AK, JR Kusel, N. Araújo, PMZ Coelho, and N. Katz. "Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni." Memórias do Instituto Oswaldo Cruz 101, suppl 1 (2006): 289–92. http://dx.doi.org/10.1590/s0074-02762006000900045.

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Bottari, Benedetta, Giovanni Campari, and Monica Gatti. "LIVE/DEAD YEAST VIABILITY STAINING AS A TOOL FOR IMPROVING ARTISANAL PILSNER BEER PRODUCTION." Journal of Microbiology, Biotechnology and Food Sciences 04, no. 02 (2014): 174–78. http://dx.doi.org/10.15414/jmbfs.2014.4.2.174-178.

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Green, John-Bruce D., Susan Bickner, Phillip W. Carter, et al. "Antimicrobial testing for surface-immobilized agents with a surface-separated live-dead staining method." Biotechnology and Bioengineering 108, no. 1 (2010): 231–36. http://dx.doi.org/10.1002/bit.22929.

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Sadeghi, Farhad, Yasaman Zamani, Kaylee Lynn Bear, and Arash Kheradvar. "Material characterization and biocompatibility of polycarbonate-based polyurethane for biomedical implant applications." RSC Advances 15, no. 11 (2025): 8839–50. https://doi.org/10.1039/d5ra00568j.

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Left: Microscopy images of CB 7.1 (top) and CF (bottom) surfaces show hard domain patterns—CF has elongated lines, while CB 7.1 has darker circular regions. Right: Live/dead staining reveals more elongated live (green) cells on CF than on CB 7.1.

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Oláh, János. "Evaluation of deep-frozen ram semen from different sheep breeds with live/dead acrosome staining." Acta Agraria Debreceniensis, no. 26 (July 16, 2007): 26–28. http://dx.doi.org/10.34101/actaagrar/26/3049.

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It was found that the Kovács – Foote staining is properly adopted to examine deep-frozen ram’s semen. Data are appropriate for comparison. Examination of one ram’s semen per breed is not enough for drawing any conclusions; therefore, I will continue this research.

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GARNER, D. L., L. A. JOHNSON, S. T. YUE, B. L. ROTH, and R. P. HAUGLAND. "Dual DNA Staining Assessment of Bovine Sperm Viability Using SYBR‐14 and Propidium Iodide." Journal of Andrology 15, no. 6 (1994): 620–29. http://dx.doi.org/10.1002/j.1939-4640.1994.tb00510.x.

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ABSTRACT: A new membrane‐permeant DNA stain, SYBR‐14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR‐14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR‐14 resulted in the nuclei of living sperm fluorescing bright green. Aliquots containing nearly all living bovine sperm were prepared using glass wool/Sephadex filtration to remove dead and damaged cells. A portion of this filtered sample was killed by unprotected freeze‐thawing and used to provide mixed

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Révay, T., A. Kovács, W. Rens, and I. Gustavsson. "Simultaneous detection of viability and sex of bovine spermatozoa." Reproduction, Fertility and Development 14, no. 6 (2002): 373. http://dx.doi.org/10.1071/rd02026.

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The viability and sex of bovine spermatozoa were simultaneously evaluated. After viability and acrosome staining with trypan blue/Giemsa, only live spermatozoa became decondensed by a modified papain-dithiothreitol method. Owing to this specific effect, live sperm heads were easily distinguished by their enlarged size and dark violet colour from small, light blue dead sperm heads. In the same sperm sample, X- and Y-chromosome-bearing sperm were distinguished by their fluorescent signal, using fluorescence in situ hybridization (FISH) with an XY paint set and 4,6-diamino-2-phenylindole counters

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Moriwaki, Takahito, Akira Yamasaki, and Qiu-Mei Zhang-Akiyama. "ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells." Oxidative Medicine and Cellular Longevity 2018 (July 2, 2018): 1–9. http://dx.doi.org/10.1155/2018/3862070.

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Introduction. Ataxia-telangiectasia-mutated (ATM) kinase is a master regulator of the DNA damage response and is directly activated by reactive oxygen species (ROSs) in addition to DNA double-stranded breaks. However, the physiological function of the response to ROSs is not understood. Purpose. In the present study, we investigated how ATM responds to ROSs in Caenorhabditis elegans (C. elegans). Materials and Methods. First, we measured sensitivities of larvae to DNA-damaging agents and ROSs. Next, we analyzed the drug sensitivities of fully matured adult worms, which consist of nondividing s

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Sun, Yan, Wentao Jiang, Mingzheng Zhang, et al. "The Inhibitory Effects of Ficin on Streptococcus mutans Biofilm Formation." BioMed Research International 2021 (March 23, 2021): 1–11. http://dx.doi.org/10.1155/2021/6692328.

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To investigate the effects of ficin on biofilm formation of conditionally cariogenic Streptococcus mutans (S. mutans). Biomass and metabolic activity of biofilm were assessed using crystal violet assay, colony-forming unit (CFU) counting, and MTT assay. Extracellular polysaccharide (EPS) synthesis was displayed by SEM imaging, bacteria/EPS staining, and anthrone method while acid production was revealed by lactic acid assay. Growth curve and live/dead bacterial staining were conducted to monitor bacterial growth state in both planktonic and biofilm form. Total protein and extracellular protein

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Low, Quentin, Veronica Calderon, Aimei Chen, et al. "Fluorescent hypoxia probe for flow cytometry." Journal of Immunology 200, no. 1_Supplement (2018): 174.32. http://dx.doi.org/10.4049/jimmunol.200.supp.174.32.

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Abstract Hypoxia is a condition where low levels of oxygen levels are present (1% to 2% O2). Hypoxia play a wide role in physiological and pathological conditions, from developmental angiogenesis to tumor progression and evasion. Hypoxia also modulates a number of immune functions from promoting inflammation to suppressing adaptive immunity. The current method for detecting hypoxic cells relies on an immunochemical approach. Pimonidazole react with peptide thiols in hypoxic cells and the resulting adduct is detected with an anti-pimonidazole antibody. To increase the availability of hypoxia de

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Nagy, Sz, A. Kovács, T. Zubor, Z. Zomborszky, J. Tóth, and P. Horn. "Evaluation of membrane integrity of frozen/thawed deer spermatozoa: Short communication." Acta Veterinaria Hungarica 49, no. 2 (2001): 223–27. http://dx.doi.org/10.1556/004.49.2001.2.12.

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A simultaneous live/dead and acrosome staining, originally described for domestic mammals, was successfully applied on red deer (Cervus elaphus) and fallow deer (Dama dama) spermatozoa collected from the cauda epididymidis and vas deferens of shot stags. The staining is simple enough for routine application. Seven classes of spermatozoa were distinguished in the smears of frozen/thawed semen samples. Morphology, including cytoplasmic droplets, was evaluated as well. Percentage of live cells with intact acrosomes and with no other morphological aberrations might be a practical index of semen qu

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Parfitt, Dan E., and S. Ganeshan. "Comparison of Procedures for Estimating Viability of Prunus Pollen." HortScience 24, no. 2 (1989): 354–56. http://dx.doi.org/10.21273/hortsci.24.2.354.

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Abstract Seven assays (hanging-drop slide and agar-plate germination, acetocarmine, three tetrazolium-based stains, and Alexander’s staining procedures) were used to estimate pollen viability in Prunus armeniaca L., P. avium L., P. dulcis Webb, P. persica (L.) Batsch, and P. salicina Lindl. The two in vitro germination tests (hanging-drop and agar-plate) were the most reliable and were highly correlated (r = 0.96). The pollen staining procedures were not reliable or consistent and were not positively correlated with the in vitro assays. Acetocarmine and Alexander’s stains stained dead pollen.

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Kessel, Martin, E. Loren Buhle, Simone Cohen, and Ueli Aebi. "Two distinct negative staining patterns of halobacterium cell envelopes." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 740–41. http://dx.doi.org/10.1017/s0424820100128018.

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The cell walls of Halobacteria are comprised of a 200,000MW sulphated glycoprotein, and by shadow casting are seen as a periodic hexagonal lattice of morphological units with a center to center spacing of approximately 140A. Preparations ot virtually salt tree envelopes from Halobacterium volcanii isolated from the Dead Sea, obtained by freezing and thawing the cells and suspension in 10mM CaCl2, have enabled high resolution analysis of the wall morphological units by negative staining.Envelopes of H. volcanii negatively stained with 1% uranyl acetate or 0.75% uranyl formate both at pH 4.5, ha

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Green, L. C., P. J. LeBlanc, and E. S. Didier. "Discrimination between Viable and Dead Encephalitozoon cuniculi (Microsporidian) Spores by Dual Staining with Sytox Green and Calcofluor White M2R." Journal of Clinical Microbiology 38, no. 10 (2000): 3811–14. http://dx.doi.org/10.1128/jcm.38.10.3811-3814.2000.

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Microsporidia are obligate intracellular parasites, recognized as causing chronic diarrhea and systemic disease in AIDS patients, organ transplant recipients, travelers, and malnourished children. Species of microsporidia that infect humans have been detected in drinking-water sources, and methods are needed to ascertain if these microsporidia are viable and capable of causing infections. In this study, Calcofluor White M2R and Sytox Green stains were used in combination to differentiate between live (freshly harvested) and dead (boiled)Encephalitozoon cuniculi spores. Calcofluor White M2R bin

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Wideman, Nathan E., James D. Oliver, Philip Glen Crandall, and Nathan A. Jarvis. "Detection and Potential Virulence of Viable but Non-Culturable (VBNC) Listeria monocytogenes: A Review." Microorganisms 9, no. 1 (2021): 194. http://dx.doi.org/10.3390/microorganisms9010194.

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The detection, enumeration, and virulence potential of viable but non-culturable (VBNC) pathogens continues to be a topic of discussion. While there is a lack of definitive evidence that VBNC Listeria monocytogenes (Lm) pose a public health risk, recent studies suggest that Lm in its VBNC state remains virulent. VBNC bacteria cannot be enumerated by traditional plating methods, so the results from routine Lm testing may not demonstrate a sample’s true hazard to public health. We suggest that supplementing routine Lm testing methods with methods designed to enumerate VBNC cells may more accurat

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Khan, Shakeel Ahmad, Sammia Shahid, Sadaf Hanif, Hesham S. Almoallim, Sulaiman Ali Alharbi, and Hanen Sellami. "Green Synthesis of Chromium Oxide Nanoparticles for Antibacterial, Antioxidant Anticancer, and Biocompatibility Activities." International Journal of Molecular Sciences 22, no. 2 (2021): 502. http://dx.doi.org/10.3390/ijms22020502.

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This study deals with the green synthesis of chromium oxide (Cr2O3) nanoparticles using a leaf extract of Abutilon indicum (L.) Sweet as a reducing and capping agent. Different characterization techniques were used to characterize the synthesized nanoparticles such as X-ray diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), Energy-dispersive X-ray (EDX), Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and ultraviolet-visible (UV-VIS) spectroscopy. The X-ray diffraction technique confirmed the purity and crystallinity of the

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Koban, Ina, Marie Henrike Geisel, Birte Holtfreter, et al. "Synergistic Effects of Nonthermal Plasma and Disinfecting Agents against Dental Biofilms In Vitro." ISRN Dentistry 2013 (September 12, 2013): 1–10. http://dx.doi.org/10.1155/2013/573262.

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Aim. Dental biofilms play a major role in the pathogenesis of many dental diseases. In this study, we evaluated the synergistic effect of atmospheric pressure plasma and different agents in dentistry on the reduction of biofilms. Methods and Results. We used monospecies (S. mutans) and multispecies dental biofilm models grown on titanium discs in vitro. After treatment with one of the agents, the biofilms were treated with plasma. Efficacy of treatment was determined by the number of colony forming units (CFU) and by live-dead staining. For S. mutans biofilms no colonies could be detected afte

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