Academic literature on the topic 'Delta subunit'

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Journal articles on the topic "Delta subunit"

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Paudel, H. K., and G. M. Carlson. "The quaternary structure of phosphorylase kinase as influenced by low concentrations of urea. Evidence suggesting a structural role for calmodulin." Biochemical Journal 268, no. 2 (1990): 393–99. http://dx.doi.org/10.1042/bj2680393.

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Skeletal-muscle phosphorylase kinase is a hexadecameric oligomer composed of equivalent amounts of four different subunits, (alpha beta gamma delta)4. The delta-subunit, which is calmodulin, functions as an integral subunit of the oligomer, and the gamma-subunit is catalytic. To learn more about intersubunit contacts within the hexadecamer and about the roles of individual subunits, we induced partial dissociation of the holoenzyme with low concentrations of urea. In the absence of Ca2+ the quaternary structure of phosphorylase kinase is very sensitive to urea over a narrow concentration range
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Saedi, M. S., W. G. Conroy, and J. Lindstrom. "Assembly of Torpedo acetylcholine receptors in Xenopus oocytes." Journal of Cell Biology 112, no. 5 (1991): 1007–15. http://dx.doi.org/10.1083/jcb.112.5.1007.

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To study pathways by which acetylcholine receptor (AChR) subunits might assemble, Torpedo alpha subunits were expressed in Xenopus oocytes alone or in combination with beta, gamma, or delta subunits. The maturation of the conformation of the main immunogenic region (MIR) on alpha subunits was measured by binding of mAbs and the maturation of the conformation of the AChR binding site on alpha subunits was measured by binding of alpha-bungarotoxin (alpha Bgt) and cholinergic ligands. The size of subunits and subunit complexes was assayed by sedimentation on sucrose gradients. It is generally acc
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Rasmussen, O. F., M. H. Shirvan, H. Margalit, C. Christiansen, and S. Rottem. "Nucleotide sequence, organization and characterization of the atp genes and the encoded subunits of Mycoplasma gallisepticum ATPase." Biochemical Journal 285, no. 3 (1992): 881–88. http://dx.doi.org/10.1042/bj2850881.

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The nucleotide sequence of a 7.8 kbp DNA fragment from the genome of Mycoplasma gallisepticum has been determined. The fragment contains a cluster of nine tightly linked genes coding for the subunits of the M. gallisepticum ATPase. The gene order is I (I-subunit), B (a-subunit), E (c-subunit), F (b-subunit), H (delta-subunit), A (alpha-subunit), G (gamma-subunit), D (beta-subunit) and C (epsilon-subunit). Two open reading frames were identified in the flanking regions; one (ORFU), preceding the I gene, encodes at least 110 amino acids and the other (ORFS), following the C gene, encodes at leas
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Wang, Z. Z., S. F. Hardy, and Z. W. Hall. "Membrane tethering enables an extracellular domain of the acetylcholine receptor alpha subunit to form a heterodimeric ligand-binding site." Journal of Cell Biology 135, no. 3 (1996): 809–17. http://dx.doi.org/10.1083/jcb.135.3.809.

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The first step of assembly of the nicotinic acetylcholine receptor (AChR) of adult skeletal muscle is the specific association of the alpha subunit with either delta or epsilon subunits to form a heterodimer with a ligand-binding site. Previous experiments have suggested that het erodimer formation in the ER arises from interaction between the luminal, NH2-terminal domains of the subunits. When expressed in COS cells with the delta subunit, however, the truncated NH2-terminal domain of the subunit folded correctly but did not form a heterodimer. Association with the delta subunit occurred only
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Gu, Y., J. R. Forsayeth, S. Verrall, X. M. Yu, and Z. W. Hall. "Assembly of the mammalian muscle acetylcholine receptor in transfected COS cells." Journal of Cell Biology 114, no. 4 (1991): 799–807. http://dx.doi.org/10.1083/jcb.114.4.799.

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We have investigated the mechanisms of assembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AChR) in transiently transfected COS cells. In cells transfected with all four subunit cDNAs, AChR was expressed on the surface with properties resembling those seen in mouse muscle cells (Gu, Y., A. F. Franco, Jr., P.D. Gardner, J. B. Lansman, J. R. Forsayeth, and Z. W. Hall. 1990. Neuron. 5:147-157). When incomplete combinations of AChR subunits were expressed, surface binding of 125I-alpha-bungarotoxin was not detected except in the case of alpha beta gamma
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Kubalek, E., S. Ralston, J. Lindstrom, and N. Unwin. "Location of subunits within the acetylcholine receptor by electron image analysis of tubular crystals from Torpedo marmorata." Journal of Cell Biology 105, no. 1 (1987): 9–18. http://dx.doi.org/10.1083/jcb.105.1.9.

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The binding sites on the nicotinic acetylcholine receptor of labels specific for the alpha-, beta-, and delta-subunits were determined by electron image analysis, using tubular crystals of receptors grown from the postsynaptic membranes of Torpedo marmorata electric organ. The labels were alpha-bungarotoxin (which attaches to the acetylcholine binding sites on the pair of alpha-subunits), Fab35 (a monoclonal antibody Fab fragment directed against the main immunogenic region of the alpha-subunit), Fab111 (a monoclonal antibody Fab fragment directed against a cytoplasmic site on the beta-subunit
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Blount, P., M. M. Smith, and J. P. Merlie. "Assembly intermediates of the mouse muscle nicotinic acetylcholine receptor in stably transfected fibroblasts." Journal of Cell Biology 111, no. 6 (1990): 2601–11. http://dx.doi.org/10.1083/jcb.111.6.2601.

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We have used fibroblast clones expressing muscle nicotinic acetylcholine receptor alpha and gamma, and alpha and delta subunits to measure the kinetics of subunit assembly, and to study the properties of the partially assembled products that are formed. We demonstrate by coimmunoprecipitation that assembly intermediates in fibroblasts coexpressing alpha and delta subunits are formed in a time-dependent manner. The alpha and gamma- and the alpha and delta-producing transfected cells form complexes that, when labeled with 125I-alpha-bungarotoxin, migrate in sucrose gradients at 6.3S, a value con
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Kashiwagi, Kazuhiro, Takuhiro Ito, and Shigeyuki Yokoyama. "Structural insight into the eIF2-eIF2B interaction." Acta Crystallographica Section A Foundations and Advances 70, a1 (2014): C1390. http://dx.doi.org/10.1107/s2053273314086094.

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eIF2B (eukaryotic initiation factor 2B) is a key regulator of translation initiation. It catalyzes guanine nucleotide exchange on eIF2, which delivers the methionylated initiator tRNA to the 40S ribosomal subunit. This exchange reaction is inhibited by the stress-induced phosphorylation of the eIF2 alpha subunit, which leads to global repression of cellular protein synthesis. eIF2B is composed of five subunits. The catalytic gamma/epsilon subcomplex is responsible for nucleotide exchange, while the regulatory alpha/beta/delta subcomplex discriminates the phosphorylation status of the eIF2 alph
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Baldwin, TJ, CM Yoshihara, K. Blackmer, CR Kintner, and SJ Burden. "Regulation of acetylcholine receptor transcript expression during development in Xenopus laevis." Journal of Cell Biology 106, no. 2 (1988): 469–78. http://dx.doi.org/10.1083/jcb.106.2.469.

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The level of transcripts encoding the skeletal muscle acetylcholine receptor (AChR) was determined during embryonic development in Xenopus laevis. cDNAs encoding the alpha, gamma, and delta subunits of the Xenopus AChR were isolated from Xenopus embryo cDNA libraries using Torpedo AChR cDNAs as probes. The Xenopus AChR cDNAs have greater than 60% amino acid sequence homology to their Torpedo homologues and hybridize to transcripts that are restricted to the somites of developing embryos. Northern blot analysis demonstrates that a 2.3-kb transcript hybridizes to the alpha subunit cDNA, a 2.4-kb
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Horak, A., H. Horak, B. Dunbar, J. E. Fothergill, and S. B. Wilson. "Plant mitochondrial F1-ATPase. The presence of oligomycin-sensitivity-conferring protein (OSCP)." Biochemical Journal 263, no. 1 (1989): 301–4. http://dx.doi.org/10.1042/bj2630301.

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Purified pea (Pisum sativum) cotyledon F1-ATPase contains six subunits rather than the five usually reported for F1-ATPases. The additional 26.5 kDa (delta) subunit is shown by immunoblotting and N-terminal amino acid sequencing to be similar to bovine oligomycin-sensitivity-conferring protein (OSCP). It is concluded that the delta subunit of plant mitochondrial F1-ATPase is the plant OSCP. This OSCP subunit occurs in all mono- and di-cotyledonous species of plants tested (maize, oats, peas, potatoes, sweet potatoes and turnips).
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Dissertations / Theses on the topic "Delta subunit"

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Cook, Terry Ann. "Function and regulation of the delta subunit of PDE6 /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/6287.

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Peden, Andrew Alexander. "Characterisation of the AP-3 adaptor-like complex." Thesis, University of Cambridge, 2000. https://www.repository.cam.ac.uk/handle/1810/243433.

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Clathrin coated vesicles were the first type of coated vesicle to be characterised. The coat consists of two components, clathrin and adaptor (or AP) complexes, the AP-1 complex is associated with the clathrin coated vesicles that bud from the TGN and the AP-2 complex is associated with the clathrin coated vesicles that bud from the plasma membrane. A new type of adaptor-like complex was discovered in our laboratory and was published in 1996. The complex has been shown to consist of two known proteins, beta3B and mu3B, and two unknown proteins of 160kD and 22kD. Unlike the conventional adaptor
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Xue, Xiaoli [Verfasser], and Irene [Akademischer Betreuer] Wagner-Döbler. "Role of the delta subunit of RNA polymerase in Streptococcus mutans / Xiaoli Xue ; Betreuer: Irene Wagner-Döbler." Braunschweig : Technische Universität Braunschweig, 2011. http://d-nb.info/117582643X/34.

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Soubrane, Camille Hélène. "Identification of CACHD1 as a novel [alpha]2[delta]-like auxiliary subunit of Cav3 voltage-gated calcium channels." Thesis, University of Reading, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658003.

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The putative CACHD1 gene was identified following a systematic search for ligand-binding proteins as novel drug targets. The CACHD1 gene encodes CACHD1 and has a predicted protein structure similar to that of the [alpha]2[delta] family of voltage-gated Ca2+ channel (VGCC) auxiliary subunits, which modulates the biophysical properties and plasma membrane expression of VGCCs. On this basis, the hypothesis that CACHD1 represents a novel [alpha]2[delta]-like VGCC auxiliary subunit was tested.
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Dobinson, Katherine Frances. "A kinetic analysis of transcription initiation by the Bacillus subtilis sigma-43 RNA polymerase : the effect of the delta subunit." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/27000.

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The initiation of transcription by the Bacillus subtilis sigma-M3 RNA polymerase at two Bacillus phage ɸ29 promoters and the effect of the delta subunit on initiation have been investigated by an in vitro kinetic analysis. The templates for the analysis were plasmids which carried the ɸ29 A2 or G2 promoter. The cloning and localization of the A2 promoter are reported here. The kinetics of RNA synthesis initiation were examined using a single-round run-off transcription assay in which multiple initiation events at a single promoter were inhibited with heparin. It was observed that the formatio
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Rosati, Marco <1983&gt. "Immunohistochemical demonstration and distribution of Voltage-Gated Calcium Channel alfa2 delta subunit in dorsal root ganglia of neonates and adults dogs." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3866/.

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Brodbeck, Jens. "Functional aspects of a mutation in the α2[delta]-2 Calcium channel subunit of the ducky mouse, a model for absence epilepsy and cerebellar ataxia." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271095.

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Hendrich, Janek K. "Alpha-2-delta subunits of voltage-gated calcium channels." Thesis, University College London (University of London), 2008. http://discovery.ucl.ac.uk/1444197/.

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The calcium channel alpha-2-delta (0,28) subunit is an auxiliary subunit associated with voltage-dependent calcium channels. It is implicated in the trafficking and functional expression of the calcium channel complex. This study expands the functional role of the VWA domain and the RRR motif of the 0:26 subunit, and the interaction between this subunit and the anti-epileptic drug, gabapentin The VWA domain is normally found in integrins, where it mediates binding to extracellular proteins. A mutation in the 0:28-2 subunit VWA domain (uMIDAS) did not produce the increase in current amplitude e
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Conte, Emanuele <1984&gt. "Funzioni della subunità θ e del dominio PHP della subunità α nel core catalitico della DNA polimerasi III di Escherichia coli". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4639/.

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Il core catalitico della DNA polimerasi III, composto dalle tre subunità α, ε e θ, è il complesso minimo responsabile della replicazione del DNA cromosomiale in Escherichia coli. Nell'oloenzima, α ed ε possiedono rispettivamente un'attività 5'-3' polimerasica ed un'attività 3'-5' esonucleasica, mentre θ non ha funzioni enzimatiche. Il presente studio si è concentrato sulle regioni del core che interagiscono direttamente con ε, ovvero θ (interagente all'estremità N-terminale di ε) e il dominio PHP di α (interagente all'estremità C-terminale di ε), delle quali non è stato sinora identificato il
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Bressanin, Daniela <1978&gt. "Studio della subunità epsilon della DNA polimerasi III di Escherichia coli: stabilità e interazione con la subunità polimerasica." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2009. http://amsdottorato.unibo.it/1593/.

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Faithful replication of DNA from one generation to the next is crucial for long-term species survival. Genomic integrity in prokaryotes, archaea and eukaryotes is dependent on efficient and accurate catalysis by multiple DNA polymerases. Escherichia coli possesses five known DNA polymerases (Pol). DNA polymerase III holoenzyme is the major replicative polymerase of the Escherichia coli chromosome (Kornberg, 1982). This enzyme contains two Pol III cores that are held together by a t dimer (Studwell-Vaughan and O’Donnell, 1991). The core is composed of three different proteins named α-, ε-
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Book chapters on the topic "Delta subunit"

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Lill, Holger, Siegfried Engelbrecht, and Wolfgang Junge. "Delta subunit of chloroplast coupling factor 1 (CF1) inhibits proton leakage through coupling factor 0 (CF0)." In Progress in Photosynthesis Research. Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0516-5_30.

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Baldwin, T. J., and S. J. Burden. "Myotube Specific Expression of the Mouse AChR Delta Subunit Gene is Conferred by 148 BP of 5′ Flanking DNA." In Molecular Biology of Neuroreceptors and Ion Channels. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74155-5_42.

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Berzborn, R. J., W. Finke, J. Otto, et al. "Partial Protein Sequence of the Subunit Delta from Spinach and Maize CF1 and Topographical Studies on the Binding Region between CF1 and CFo." In Progress in Photosynthesis Research. Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0516-5_22.

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Marx, Alexander, Annette Wilisch, Stefanie Gutsche, et al. "Low Levels of Acetylcholine Receptor Delta-Subunit Message and Protein in Human Thymus Suggests the Occurrence of ‘Triplet Receptors’ in Thymic Myoid Cells." In Myasthenia Gravis. Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4060-7_3.

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Piergiorgio, Strata, and Mandolesi Georgia. "The Glutamate Receptor delta2 Subunit in Cerebellar Wiring." In Cerebral Plasticity. The MIT Press, 2011. http://dx.doi.org/10.7551/mitpress/9780262015233.003.0012.

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Conference papers on the topic "Delta subunit"

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Schwagerus, Elena, Stephen T. Buckley, and Carsten Ehrhardt. "ENaC Delta-subunit Is Functionally Expressed In Human Respiratory Epithelial Cells." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a6454.

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Schwagerus, Elena, Beate Illek, Horst Fischer, and Carsten Ehrhardt. "Functional Expression Of Epithelial Sodium Channel Delta Subunit In Human Respiratory Epithelial Cells In Vitro." In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a3528.

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Schwagerus, Elena, Stephen T. Buckley, and Carsten Ehrhardt. "Expression And Function Of The Epithelial Sodium Channel Delta Subunit In Human Respiratory Epithelial Cell Lines." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a4229.

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