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Dissertations / Theses on the topic 'Delta subunit'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Cook, Terry Ann. "Function and regulation of the delta subunit of PDE6 /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/6287.

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2

Peden, Andrew Alexander. "Characterisation of the AP-3 adaptor-like complex." Thesis, University of Cambridge, 2000. https://www.repository.cam.ac.uk/handle/1810/243433.

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Clathrin coated vesicles were the first type of coated vesicle to be characterised. The coat consists of two components, clathrin and adaptor (or AP) complexes, the AP-1 complex is associated with the clathrin coated vesicles that bud from the TGN and the AP-2 complex is associated with the clathrin coated vesicles that bud from the plasma membrane. A new type of adaptor-like complex was discovered in our laboratory and was published in 1996. The complex has been shown to consist of two known proteins, beta3B and mu3B, and two unknown proteins of 160kD and 22kD. Unlike the conventional adaptor

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3

Xue, Xiaoli [Verfasser], and Irene [Akademischer Betreuer] Wagner-Döbler. "Role of the delta subunit of RNA polymerase in Streptococcus mutans / Xiaoli Xue ; Betreuer: Irene Wagner-Döbler." Braunschweig : Technische Universität Braunschweig, 2011. http://d-nb.info/117582643X/34.

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4

Soubrane, Camille Hélène. "Identification of CACHD1 as a novel [alpha]2[delta]-like auxiliary subunit of Cav3 voltage-gated calcium channels." Thesis, University of Reading, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658003.

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The putative CACHD1 gene was identified following a systematic search for ligand-binding proteins as novel drug targets. The CACHD1 gene encodes CACHD1 and has a predicted protein structure similar to that of the [alpha]2[delta] family of voltage-gated Ca2+ channel (VGCC) auxiliary subunits, which modulates the biophysical properties and plasma membrane expression of VGCCs. On this basis, the hypothesis that CACHD1 represents a novel [alpha]2[delta]-like VGCC auxiliary subunit was tested.

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5

Dobinson, Katherine Frances. "A kinetic analysis of transcription initiation by the Bacillus subtilis sigma-43 RNA polymerase : the effect of the delta subunit." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/27000.

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The initiation of transcription by the Bacillus subtilis sigma-M3 RNA polymerase at two Bacillus phage ɸ29 promoters and the effect of the delta subunit on initiation have been investigated by an in vitro kinetic analysis. The templates for the analysis were plasmids which carried the ɸ29 A2 or G2 promoter. The cloning and localization of the A2 promoter are reported here. The kinetics of RNA synthesis initiation were examined using a single-round run-off transcription assay in which multiple initiation events at a single promoter were inhibited with heparin. It was observed that the formatio

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6

Rosati, Marco <1983&gt. "Immunohistochemical demonstration and distribution of Voltage-Gated Calcium Channel alfa2 delta subunit in dorsal root ganglia of neonates and adults dogs." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3866/.

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7

Brodbeck, Jens. "Functional aspects of a mutation in the Î±2[delta]-2 Calcium channel subunit of the ducky mouse, a model for absence epilepsy and cerebellar ataxia." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271095.

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8

Hendrich, Janek K. "Alpha-2-delta subunits of voltage-gated calcium channels." Thesis, University College London (University of London), 2008. http://discovery.ucl.ac.uk/1444197/.

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The calcium channel alpha-2-delta (0,28) subunit is an auxiliary subunit associated with voltage-dependent calcium channels. It is implicated in the trafficking and functional expression of the calcium channel complex. This study expands the functional role of the VWA domain and the RRR motif of the 0:26 subunit, and the interaction between this subunit and the anti-epileptic drug, gabapentin The VWA domain is normally found in integrins, where it mediates binding to extracellular proteins. A mutation in the 0:28-2 subunit VWA domain (uMIDAS) did not produce the increase in current amplitude e

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9

Conte, Emanuele <1984&gt. "Funzioni della subunità θ e del dominio PHP della subunità α nel core catalitico della DNA polimerasi III di Escherichia coli". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4639/.

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Il core catalitico della DNA polimerasi III, composto dalle tre subunità α, ε e θ, è il complesso minimo responsabile della replicazione del DNA cromosomiale in Escherichia coli. Nell'oloenzima, α ed ε possiedono rispettivamente un'attività 5'-3' polimerasica ed un'attività 3'-5' esonucleasica, mentre θ non ha funzioni enzimatiche. Il presente studio si è concentrato sulle regioni del core che interagiscono direttamente con ε, ovvero θ (interagente all'estremità N-terminale di ε) e il dominio PHP di α (interagente all'estremità C-terminale di ε), delle quali non è stato sinora identificato il

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10

Bressanin, Daniela <1978&gt. "Studio della subunità epsilon della DNA polimerasi III di Escherichia coli: stabilità e interazione con la subunità polimerasica." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2009. http://amsdottorato.unibo.it/1593/.

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Faithful replication of DNA from one generation to the next is crucial for long-term species survival. Genomic integrity in prokaryotes, archaea and eukaryotes is dependent on efficient and accurate catalysis by multiple DNA polymerases. Escherichia coli possesses five known DNA polymerases (Pol). DNA polymerase III holoenzyme is the major replicative polymerase of the Escherichia coli chromosome (Kornberg, 1982). This enzyme contains two Pol III cores that are held together by a t dimer (Studwell-Vaughan and O’Donnell, 1991). The core is composed of three different proteins named α-, ε-

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11

Robinson, Philip. "Targeting of voltage-gated calcium channels to lipid rafts : the role of auxiliary alpha2/delta-1 subunits." Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/targeting-of-voltagegated-calcium-channels-to-lipid-rafts-the-role-of-auxiliary-alpha2delta1-subunits(db84049c-1445-4486-9e31-34fa4110daf6).html.

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Ca2+ entry through voltage-gated calcium channels (CaVs) triggers a range of physiological events, including synaptic neurotransmission and muscular excito-contraction coupling. CaVs are often localised to discrete membrane microdomains and are required to be targeted to such fine structures in order to perform their cellular functions. CaVs are multi-subunit protein complexes that consist of a core, pore-forming α1 subunit and auxiliary β and α2/δ subunits. The α2/δ subunit is required for the optimal cell surface expression and function of CaVs and is itself localised to cholesterol-rich mem

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12

Perrone, Alessandro. "Sovraespressione, purificazione e caratterizzazione cinetica della polimerasi DnaE di Deinococcus radiodurans." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/10017/.

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Il passo preliminare del presente elaborato è quello di mettere a punto un metodica solida e riproducibile per la sovraespressione della subunità α di D. radiodurans per via eterologa. L’ospite ideale per realizzare la sovraespressione è E. coli, microorganismo ampiamente discusso e impiegato per via della sua semplice manipolazione. Per realizzare la sovraespressione il ceppo di E. coli prescelto è stato trasformato con il plasmide pBAD-dnaE e la proteina in esame è stata ottenuta come corpi di inclusione (biologicamente inattiva). Successivamente alla fase di rinaturazione e alla purificazio

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13

Kim, Adonia Lee. "The Role of Adaptor Protein Complex-3 Delta-Mediated HIV-1 Gag Trafficking in HIV-1 Replication: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/612.

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The process of HIV-1 particle production is a multi-step process directed by the viral structural protein Gag. As Gag is the only viral protein required to form virus-like particles, it presents a viable target for anti-viral therapeutics of which there are currently none. Although the functions of Gag during the particle assembly process have been well characterized, one of the least known parts of the assembly process is how Gag is targeted to the site of virus assembly. Two main virus assembly sites have been identified in cells that support HIV-1 replication: the plasma membrane or multive

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14

Rabatinová, Alžběta. "Regulace traskripce u gram-pozitivních bakterií." Doctoral thesis, 2021. http://www.nusl.cz/ntk/nusl-438157.

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Bacteria are the most abundant organisms on the planet. They live almost in all environments, including those that are most extreme. All land and water ecosystems depend heavily upon their activity. Bacteria play essential roles in cycling of nutrients such as carbon, nitrogen, and sulphur. Due to their short cell cycle, they must be able to swiftly adapt to the conditions of their habitat to survive. Microbial growth itself is an autocatalytic process. There are three distinct phases of the growth curve: lag, exponential (log), and stationary. Bacterial cells must change their gene expression

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15

Härteis, Silke [Verfasser]. "Effects of the δ-subunit [delta-subunit] and of proteolytic channel cleavage on the function of the epithelial sodium channel (ENaC) / vorgelegt von Silke Härteis". 2010. http://d-nb.info/999751220/34.

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16

Bruning, John. "Structural and thermodynamic analysis of human PCNA bound to peptides derived from DNA polymerase-delta p66-subunit and flap endonuclease-1 (FEN1) proteins." Thesis, 2005. http://hdl.handle.net/1911/18741.

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DNA replication and repair requires the coordination of multiple proteins at the replication fork. Among the proteins found at the replisome, hPCNA (h&barbelow;uman P&barbelow;roliferating C&barbelow;ell N&barbelow;uclear A&barbelow;ntigen) is essential for many of the protein-protein interactions required for replication and repair processes. hPCNA is a member of the sliding clamp family of DNA replication processivity factors. Recent studies have shown that hPCNA is able to make specific protein-protein interactions to a wide range of DNA replication, recombination and repair proteins throug

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17

Jirát, Matějčková Jitka. "Interakce vybraných bílkovin s RNA polymerázou z Bacillus subtilis." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-309370.

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