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Dissertations / Theses on the topic 'Denaturing gradient gel electrophoresis (DGGE)'

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Published: 4 June 2021

Last updated: 26 July 2025

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1

Cadiou, Helene. "Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection." Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418093.

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Keyser, Maricel. "PCR detection, denaturing gradient gel electrophoresis (DGGE) fingerprinting and identification of the microbial consortium in different types of UASB granules." Thesis, Link to online version, 2006. http://hdl.handle.net/10019/558.

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3

Venter, Leandra. "Presence of potentially pathogenic heterotrophic plate count (HPC) bacteria occurring in a drinking water distribution system in the North-West Province, South Africa / by Leandra Venter." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4380.

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There is currently growing concern about the presence of heterotrophic plate count (HPC) bacteria in drinking water. These HPC may have potential pathogenic features, enabling them to cause disease. It is especially alarming amongst individuals with a weakened immune system. South Africa, the country with the highest incidents of HIV positive individuals in the world, mainly uses these counts to assess the quality of drinking water in terms of the number of micro-organisms present in the water. These micro-organisms may be present in the bulk water or as biofilms adhered to the surfaces of a d

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4

Lindelof, Kara L. "Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population." University of Toledo / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544.

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5

Le, Nguyen Doan Duy. "Détermination de l'origine géographique des poissons par l'obtention de l'empreinte génétique de leur communauté bactérienne par PCR-DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis)." Montpellier 2, 2008. http://www.theses.fr/2008MON20103.

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La détermination de l'origine géographique est une des exigences de la traçabilité des produits alimentaires. Notre hypothèse était que l'environnement de l'aliment pouvait avoir une influence sur la diversité des flores bactériennes commensales des poissons. L'origine géographique des poissons a donc été approchée par la caractérisation de la flore microbienne spécifique de la ferme de production ou du lieu de transformation et de stockage. Une technique de biologie moléculaire couplée à une analyse d'images et à des méthodes statistiques a été développée afin de relier les profils bactériens

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6

Theunissen, Johnita. "Identification of probiotic microbes from South African products using PCR-based DGGE analyses." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/49983.

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Thesis (MScFoodSc)--Stellenbosch University, 2004.<br>ENGLISH ABSTRACT: The regular consumption of probiotics is becoming a recognized trend in the food industry due to several reported health benefits. A probiotic is defined as a live microbial feed supplement that beneficially affects the host animal by improving its intestinal microbial balance. A wide variety of probiotic food products are available on the South African market and comprise an assortment of fermented milks, as well as lyophilized preparations in tablet or capsule form. Strains of Lactobacillus acidophilus and Bifid

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Le, Riche Mia. "Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis." Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/18218.

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Assignment (MMed)--University of Stellenbosch, 2007.<br>ENGLISH ABSTRACT: Lipoprotein X (LpX) is an abnormal cholesterol-containing particle that may be present in the serum of subjects with cholestasis, lecithin:cholesterol acyltransferase (LCAT) deficiency and parenteral nutrition. The biochemistry, metabolism, clinical significance and laboratory analysis of LpX is discussed in this study. This laboratory-based project investigated icteric samples received at the Chemical Pathology laboratory, Tygerberg Hospital, for serum predictors of LpX and the use of a modified non-denaturing polyacryl

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8

Surridge, Angela Karen Joanna. "Denaturing gradient gel electrophoresis characterisation of microbial communities in polycyclic aromatic hydrocarbon and polychlorinated biphenyl contaminated soil." Thesis, Pretoria : [s.n.], 2007. http://hdl.handle.net/2263/25070.

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Fossil fuels are currently the primary industrial energy source on Earth. They are principally composed of complex hydrocarbons in either long-chain or cyclic conformation. Industrial use of petroleum, diesel, oil, tar and other coal-derived products inevitably leads to pollution of the environment. The most serious pollution is caused by polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) that are not easily removed from soil after a spill. Long-chain and cyclic conformation makes fossil fuel hydrocarbons difficult to break down. However, certain free-living soil micr

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9

Ellwood-Thompson, Rhianedd Eleri. "Occurrence and transmission of Wolbachia endosymbionts in the oak gall wasp community : application of denaturing gradient gel electrophoresis." Thesis, Cardiff University, 2004. http://orca.cf.ac.uk/55379/.

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Eight Wolbachia variants were identified in the wasp community. Identical Wolbachia variants were identified in inquiline and parasitoid wasp species suggesting that horizontal transmission of Wolbachia occurs in this community

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10

Strandgren, Charlotte. "Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis." Thesis, Linköpings universitet, Institutionen för klinisk och experimentell medicin, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15560.

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Allergic diseases, for example asthma and eczema, are nowadays considered belonging to the most common chronic diseases amongst children in the West, but the cause for this increase in allergy prevalence is unknown. Since studies have indicated a connection between children's exposure of microorganisms during infancy and risk of developing allergic disease, it is suggested that this exposure is a crucial factor in question of allergy development or not. Other studies have established differences in microflora composition between healthy children and children with allergic disease, and several

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11

PRUDEN, AMY J. "BIODEGRADATION OF METHYL TERT -BUTYL ETHER." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027943573.

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12

Van, Niekerk Bertina Freda. "Functional and structural diversity of the microbial communities associated with the use of Fischer–Tropsch GTL Primary Column Bottoms as process cooling water / van Niekerk B.F." Thesis, North-West University, 2011. http://hdl.handle.net/10394/7284.

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Despite emerging water shortages, most water is only used once, and often with low efficiency. However, with appropriate treatment, water can be re–used to reduce the demand on freshwater sources. The Department of Water Affairs, South Africa, promotes industries to reduce discharges into water resources in order to sustain an overall good water quality of all water systems. All of this ultimately leads to industries striving towards zero effluent discharge. Primary Column Bottoms (PCBs) is a wastewater stream derived from the Fischer–Tropsch Gas to Liquid process and consists mainly of organi

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13

Čakajdová, Martina. "Analýza mikroflóry v sýrech pomocí DGGE." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217120.

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Molecular biological methods are fast and efficient tool for the identification of microorganisms in real samples. The aim of this diploma thesis was analysis of microflora in control and contaminated brined cheeses. DNA isolated from analyzed samples was used to optimize the PCR course using primers with GC clamp on the distribution of amplicons using DGGE. DGGE products were reamplified after optimization and prepared for DNA sequencing. DNA isolated from analyzed samples was used in real-time PCR with high resolution melt analysis of the amplicons (HRMA). Samples of cheese and bacterial cul

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Du, Plessis Gerda. "Actinobacterial diversity of the Ethiopian Rift Valley lakes." University of the Western Cape, 2011. http://hdl.handle.net/11394/5385.

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>Magister Scientiae - MSc<br>The class Actinobacteria consists of a heterogeneous group of filamentous, Gram-positive bacteria that colonise most terrestrial and aquatic environments. The industrial and biotechnological importance of the secondary metabolites produced by members of this class has propelled it into the forefront of metagenomics studies. The Ethiopian Rift Valley lakes are characterized by several physical extremes, making it a polyextremophilic environment and a possible untapped source of novel actinobacterial species. The aims of the current study were to identify and compare

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Barnes, Kwasi H. "Diversity and Distribution of Diatom Endosymbionts in Amphistegina spp. (Foraminifera) Based on Molecular and Morphological Techniques." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6177.

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Diatoms associated with foraminifers of the genus Amphistegina were assessed using a combination of morphological and molecular techniques. These included: 1) microscopic identification of diatoms cultured from the host, 2) sequencing of portions of the small subunit of the ribosomal RNA gene (18S) and the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase [i.e., RubisCO] gene (rbcL) from DNA extracted directly from the Amphistegina hosts and also from diatoms cultured from these hosts, and 3) denaturing gradient gel electrophoresis (DGGE) profiles of rbcL and internal transc

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Otero, Walter Guimarães. "Avaliação da diversidade microbiana e degradabilidade in situ em animais tratados com preparado de anticorpos policlonais contra bactérias produtoras de lactato e bactérias proteolíticas." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/10/10135/tde-23012009-111436/.

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A imunidade passiva surge como uma alternativa para a manipulação da fermentação ruminal e anticorpos de origem aviária contra bactérias específicas começam a ser pesquisados. Objetivou-se com este trabalho avaliar um preparado de anticorpos policlonais contra Streptococus bovis, Fusobacterium necrophorum e algumas cepas de bactérias proteolíticas (Peptostreptococcus anacrobius, Clostridium aminophilum e Clostridium sticklandii) sobre a diversidade microbiana ruminal e degradabilidade in situ de alguns alimentos. Foram utilizadas nove vacas mestiças portadoras de cânula ruminal. O delineamento

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17

Ranasinghe, Purnika Damindi. "Use of next generation sequencing for analysing taxonomical and functional composition of bacteria in an insect gut microbiome." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/116377/1/Purnika%20Damindi_Ranasinghe_Thesis.pdf.

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This thesis describes the difference between the baseline composition of Diamondback moth (Plutella xylostella L.) insect colonies' gut microbiomes collected from field- and laboratory-reared populations. Results indicates that variation of gut bacterial community composition between individual insect influences baseline microbiome composition estimates at the population scale. The gut bacteria diversity and functional composition change in response to external challenges, and those changes reflect on insect development. This explains the need to discover the specific symbiotic gut bacteria th

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18

Passig, Fernando Hermes. "Reator anaeróbio híbrido para tratamento de esgoto sanitário." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-11042016-151713/.

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Este trabalho de investigação refere-se ao uso do reator anaeróbio híbrido para tratamento de esgoto sanitário, com configuração baseada no reator anaeróbio de manta de lodo (UASB) com inclusão de: meio suporte sobre as calhas de coleta de gás (denominado reator anaeróbio híbrido - UAHB) e, também, meio suporte na zona de reação (denominado reator anaeróbio híbrido modificado - UAHBmod). Para o desenvolvimento desta pesquisa, no Campus I da USP de São Carlos-SP foram construídos dois reatores experimentais de 18,8 m3 cada: um reator UASB, com função de controle, e um reator UAHB. Primeiramente

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Christophersen, Claus. "Grain and artificial stimulation of the rumen change the abundance and diversity of methanogens and their association with ciliates." University of Western Australia. School of Animal Biology, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0114.

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[Truncated abstract] In Australia, there is pressure to reduce the amount of methane produced by ruminant livestock because they are the single largest source of methane emitted from anthropogenic sources, accounting for 70.7% of agricultural methane emissions. In addition, methane production represents a loss of gross energy intake to the animal. The organisms that are responsible for methane production in the animal gut are a distinct group of Archaea called methanogens. Methanogens occupy three different niches within the rumen. Some live freely in the rumen digesta (planktonic), others are

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Khan, Ghazanfar Ali. "Monitoring anti-infectives and antibiotic resistance genes : with focus on analytical method development, effects of antibiotics and national perspectives." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-61682.

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Antibiotics are biologically active and are globally used in humans and animal medicine for treatment and in sub-therapeutic amounts as growth promoters in animal husbandry, aquaculture and agriculture. After excretion, inappropriate disposal and discharge from drug production facilities they enter into water bodies either as intact drugs, metabolites or transformed products. In water environments they promote development of antibiotic resistance genes (ARGs) which could serve as a reservoir and be horizontally transferred to human-associated bacteria and thus contribute to AR proliferation. M

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21

Wu, Chung-Yi, and 吳宗益. "Using Capillary Electrophoresis(CE) and Denaturing Gradient Gel Electrophoresis (DGGE) for Determing the Distribution of Bacteria Resistant to Kanamycin." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/74531331309891977489.

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碩士<br>朝陽科技大學<br>應用化學系碩士班<br>97<br>In recent years due to environmental changes, and more by the disruption of soil microbes, the soil bacteria to explore the importance of changing the subject. Early-related technologies such as: RAPD, RFLP, DGGE, RISA ... and have been used for analysis of bacteria, many of these technologies to 16S rDNA-based in recent years to 16S-23S (900bp ~ 1100bp) rDNA (ITS, 0.5~1.4 Kb ) carried out research is also increasing. In this study, culture method (selective medium LB, LB + Kan + Cyc) and chemical methods (CE and DGGE) for the cultivation of genetically modifi

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22

Lin, Huei-Ming, and 林徽鳴. "Monitoring molecular fingerprinting of microbial community dynamics by denaturing gradient gel electrophoresis(DGGE) and terminal-restriction fragment length polymorphism (T-RFLP)." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/56892104243407930869.

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碩士<br>國立成功大學<br>生命科學系碩博士班<br>93<br>Soil bacteria are essential components of the biotic community in natural forests and they are largely responsible for ecosystem function, and participate in the elements circulation. Although the main diversity of life has been proven to be microbial, the vast majority of soil bacteria still remain unknown for less than 1% of environmental microorganisms can be cultured. In this study, cloning method, denaturing gradient gel electrophoresis (DGGE) and terminal-restriction fragment length polymorphism (T-RFLP) techniques were used to compare the prevalent mic

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Chhun, Aline. "Molecular Characterization of Toxic Cyanobacteria in North American and East African Lakes." Thesis, 2007. http://hdl.handle.net/10012/3341.

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Toxic cyanobacterial blooms constitute a threat to the safety and ecological quality of aquatic environments worldwide. Cyclic hepatotoxin, especially microcystin, is the most widely occurring of the cyanotoxins. The aim of this study was to identify the cyanobacterial genotypes present including how many toxic genotypes were present in two North American lakes and one African Lake. All three lakes are prone to cyanobacterial blooms and were sampled in 2005 and 2006: Lake Ontario (Bay of Quinte, Canada), Lake Erie (Maumee Bay, Canada) and Lake Victoria (Nyanza Gulf, Kenya). The cyanobacterial

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Lin, Yu-Jia, and 林裕家. "Monitoring the dynamic changes of microbial community during bioremediation of oil-polluted sites by denaturing gradient gel electrophoresis (DGGE) and real-time PCR." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/76950060699574836498.

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碩士<br>國立成功大學<br>生命科學系碩博士班<br>94<br>Petroleum hydrocarbons are the most widespread contaminants in the environment. Several of petroleum hydrocarbons are known mutagens or carcinogens for human and other organisms. They are difficult to be degraded in the natural environment. Bioremediation is considered a useful method to degrade the petroleum hydrocarbons using the oil-degrading bacteria. In this study, cultivation-independent methods were used to study the microbial communities in petroleum contaminated sites. Phylogenetic analysis of bacterial 16S rRNA gene clone libraries from the LY、FD an

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25

Yergeau, Étienne. "Caractérisation moléculaire de la biodiversité des Fusarium associés à la fusariose de l'asperge (Asparagus officinalis L.) au Québec." Thèse, 2004. http://hdl.handle.net/1866/15020.

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Kennedy, Katherine Margaret. "Molecular methods for evaluating the human microbiome." Thesis, 2014. http://hdl.handle.net/10012/8230.

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In human microbiome analysis, sequencing of bacterial 16S rRNA genes has revealed a role for the gut microbiota in maintaining health and contributing to various pathologies. Novel community analysis techniques must be evaluated in terms of bias, sensitivity, and reproducibility and compared to existing techniques to be effectively implemented. Next- generation sequencing technologies offer many advantages over traditional fingerprinting methods, but this extensive evaluation required for the most efficacious use of data has not been performed previously. Illumina libraries were generated from

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Bissonnette, Laurence. "Efficacité d'espèces ligneuses en symbiose mycorhizienne arbusculaire pour la phytoremédiation d'un site urbain contaminé." Thèse, 2009. http://hdl.handle.net/1866/8122.

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Chang, Wen-Hsin, and 張文欣. "Monitoring the Lactobacillus population in broiler gastrointestinal tract by Denaturing Gradient Gel Electrophoresis." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/78987965429006419225.

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碩士<br>國立中興大學<br>動物科學系所<br>100<br>An understanding of the dynamics of bacterial community structure in the chicken intestine is essential for selection of diets for optimal nutrition, effective treatment of enteric pathogens, and the development of competitive exclusion products. The microbial diversity, count of bacteria and classification of bacteria in microbiology are many ways to quantitative and qualitative. It’s used in research on PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis), that a more convenient way to explore the changes in the composition of flora. I

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Yu-Fen, Wang, and 王郁芬. "Identification of Non-Tuberculous Mycobacterium in Environmental Samples by PCR-Denaturing Gradient gel Electrophoresis." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/77534514601538220014.

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碩士<br>國立中興大學<br>環境工程學系<br>93<br>Nontuberculous mycobacteria (NTM) is a common microorganisms which cause diseases in immunocompromised patients. In addition, Some species may cause infections similar to tuberculosis in humans and animals.There is very limited documentation of person-to-person transmission of NTM. This aim of this study is to develop a method which can detect the NTM of the tap water of hospital. In our study, we try to combine some molecular techniques based on the 16S rRNA and the hsp gene of mycobacteria, PCR-DGGE，for identification of NTM in environmental samples. 30 pure c

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Dutta, Partha. "Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16s rRNA genes." Thesis, 2006. http://hdl.handle.net/10057/369.

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Many studies of hyper saline environments have been performed, mainly on aquatic systems. However, the microbial community in terrestrial thallasohaline environments has not been studied extensively. To our knowledge, this is the first study of a natural terrestrial thallasohaline environment using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). We studied the great salt plains (GSP) for this purpose. The GSP is a perfect example of an extreme environment. The environment of the GSP changes frequently due to rain events that can change the salinity of the so

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Chen, Hsi-Chia, and 陳希嘉. "Study on identification of lactic acid bacteria by denaturing gradient gel electrophoresis and oligonucleotide microarray." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/69162826021385651302.

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博士<br>國立臺灣大學<br>動物科學技術學研究所<br>97<br>Lactic acid bacteria (LAB) are widely employed as starter cultures in the dairy industry and supplemented in different commercial products. Detecting and identifying various species of lactic acid bacteria with rapid method is often important for quality control of dairy products and monitoring fermentation processes. However, identification of lactic acid bacteria in the complex bacterial communities is still difficult for both phenotypic methods and genotypic methods. The aim of this study focused on polymerase chain reaction-denaturing gradient gel elect

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Yang, Ju-Yu, and 楊如玉. "Analysis of the Changes of Phenol Degraders by Denaturing Gradient Gel Electrophoresis During Phenol Degradation in soils." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/65267362373707710605.

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碩士<br>嘉南藥理科技大學<br>環境工程與科學系碩士班<br>93<br>Abstract Phenol is a common pollutant from industries, it impacts on the aquatic ecosystem and environmental balance extremely, and biodegradation is a very economic technique, which is the best choice of the phenol contaminated soil remediation. The phenol high-tolerance degraders were isolated from collected sludge and soil from petrol station and around of reactor and wastewater storage in Syndyne Industry, the tested strains in this study including M1 and M5. The physiological properties of te sted strains were analyzed by Gram stain, activity of cat

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Cheng, Chiu-Yu, and 鄭秋玉. "Analysis of the Changes of Bacteria Communities by Denaturing Gradient Gel Electrophoresis Method During DEHP Degradation in Soil." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/78677809950701092272.

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Tzeng, Kai-Jiun, and 曾楷珺. "Identification of Environmental Acanthamoeba and Legionella by Denaturing Gradient Gel Electrophoresis and the Influence of Antibiotics Addition for Acanthamoeba Cultivation." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/cn93e9.

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碩士<br>國立中正大學<br>應用地球物理研究所<br>102<br>Acanthamoeba,Naegleria and Hartmanella are free-living amoebae. They are widely present in various environmental water. Some amoeba species of animals and humans will cause disease, and as Legionella host, they will raising the risk of infection Legionella's disease. The purpose of this study was to investigate the distribution of water environment in Taiwan free-type amoeba and Legionella species, and the use of real-time quantitative polymerase chain reaction (Real-time qPCR) to quantitative analysis. L. pneumophila separation from the environment is the u

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Chen, Shin-Chih, and 陳信志. "Application of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis in the Detection and Identification of Lactobacillus, Staphylococcus and Alicyclobacillus Species." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/94906117638451095396.

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博士<br>國立中興大學<br>食品暨應用生物科技學系<br>96<br>Denaturing gradienet gel electrophoresis (DGGE) is a technique able to differentiate DNA fragnments with sequences diversity. With polymerase chain reaction (PCR), DGGE was able to analyze the species in mixed cultures. tuf gene is responsible for the synthesis of bacterial elongation factor Tu. The gene is a housekeeping gene and exists only one copy in Gram positive bacteria. It had been proven to possess more diversity than 16S rDNA between species in several genera. As a result, We tried to design genus-specific (or group-specific) primers from tuf ge

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浅川, 晋. "水田土壌の主要なメタン生成古細菌群の解析". 2004. http://hdl.handle.net/2237/13126.

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Ward, Rachel Jane. "The use of denaturing gradient gel electrophoresis in examining the species-specific influence of ectomycorrhizal fungi on selective bacteria enrichment in the mycorrhizosphere of Pinius rigida grown in a natural pine barrens habitat." 2007. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16793.

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Verastegui, Pena Yris Milusqui. "Targeting novel soil glycosyl hydrolases by combining stable isotope probing and metagenomics." Thesis, 2014. http://hdl.handle.net/10012/8282.

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Soil represents the largest global reservoir of microbial diversity for the discovery of novel genes and enzymes. Both stable-isotope probing (SIP) and metagenomics have been used to access uncultured microbial diversity, but few studies have combined these two methods for accessing the biotechnological potential of soil genetic diversity and fewer yet have employed functional metagenomics for recovering novel genes and enzymes for bioenergy or bioproduct applications. In this research, I demonstrate the power of combining functional metagenomics and SIP using multiple plant-derived carbon sub

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