Dissertations / Theses on the topic 'Dengue viruses'
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Li, Mingyuan, and 李明圓. "Molecular dissection of dengue virus egress : involvement of host cellular factors-KDEL receptors." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208011.
Full textpublished_or_final_version
Public Health
Doctoral
Doctor of Philosophy
Bletchly, Cheryl. "Antigenic and structural analysis of the NS1 glycoprotein of dengue virus /." St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16420.pdf.
Full textLo, Chung-yan Joanne, and 羅頌恩. "Characterization by electron microscopy of dengue virus egress using dengue recombinant subviral particle (RSPs) as a model." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48330115.
Full textpublished_or_final_version
Pathology
Master
Master of Philosophy
Warke, Rajas V. "Activation of TNF alpha, IL1-beta and Type-i IFn pathways in human umbilical vein endothelial cells during dengue 2 virus infection." Link to electronic thesis, 2002. http://www.wpi.edu/Pubs/ETD/Available/etd-0424102-141341.
Full textBhatia, Kanika Devi. "The role of mononuclear phagocytes in dengue immunopathogenesis /." St. Lucia, Qld, 2001. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17170.pdf.
Full textLambeth, Cassandra Rashida De Silva Aravinda Manu. "Interactions between dengue type 3 viruses and human dendritic cells." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1238.
Full textTitle from electronic title page (viewed Mar. 26, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Microbiology and Immunology in the School of Medicine." Discipline: Microbiology and Immunology; Department/School: Medicine.
Serafin, Ina Loretta. "Epitope mapping of the dengue 3 envelope protein." Thesis, Queensland University of Technology, 1999.
Find full textBrown, Jennifer L. "A molecular and immunological investigation of cellular responses to dengue virus identification of potentially upregulated host genes and the constructionof a vaccinia virus expressing the dengue 1 Hawaii NS3 protein." Link to electronic version, 2000. http://www.wpi.edu/Pubs/ETD/Available/etd-0330100-124248/.
Full textMatusan, Anita Esther 1973. "Mutational analysis of the proteinase and helicase regions of the Dengue virus type 2 NS3 protein." Monash University, Dept. of Microbiology, 2001. http://arrow.monash.edu.au/hdl/1959.1/8339.
Full textKudelko, Mateusz Aleksander. "Characterization of a novel role for class-II ADP-ribosylation factorsin the regulation of dengue egress using newly developed recombinantsubviral particles." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46455620.
Full textChoi, Wai-yee Junet. "Serum neopterin for early assessment of severity of severe acute respiratory syndrome and Dengue virus infection." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B32031579.
Full textEnguehard, Margot. "Interaction between chikungunya and dengue viruses during co-infection in Aedes mosquito cells and in Aedes aegypti mosquito." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1161/document.
Full textEmergence and geographical extension of dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses increase simultaneous outbreak in an increasing number of countries. To date, no vaccine or cure have yet been developed against these diseases those cause a tremendous impact on human health and in the economy worldwide. During recent simultaneous outbreaks, up to 12% of patients have been diagnosed to be co-infected by CHIKV and DENV. In addition, it was shown that the mosquitoes Aedes albopictus could carry and transmit simultaneously CHIKV and DENV. However, the pathology, as well as the epidemiology of a pathogen, relies on the interactions between several infectious agents present within an organism or a community in the environment. It is crucial to consider to which extent a host infected by a first microorganism is modified and whether its reaction to the infection by a second microorganism is consequently altered. However, there is no extensive report of Alphavirus-Flavivirus or Flavivirus- Flavivirus interactions. Our global objective is to characterize these co-infections in both mosquitoes and humans, at the cell and molecular level. To this aim, we started this project by performing sequential co- infection in different cell lines from Aedes albopictus and Aedes aegypti. We found that the permissiveness and production of DENV is enhanced in presence of CHIKV. On the contrary, there is no effect of DENV pre-infection on subsequent CHIKV co-infection. We generalized the synergistic phenomena and we showed that CHIKV pre-infection also increased the infection by DENV-1, DENV-3 and DENV-4, but also by two others re-emerging Flaviviruses, the Yellow Fever Virus (YFV), and the Zika Virus (ZIKV). Remarkably, we succeeded to establish a mosquito model of co-infection of Aedes aegypti mosquito after by different two feedings at 4 days interval. Using this sequential co-infection, we were able to show that a pre-infection of Aedes aegypti by CHIKV increase the level of DENV-2 RNA in salivary glands compare to mono-infected mosquitos. This phenotype is reminiscent of the phenotype we observed in vitro during successive infections. Altogether, our study paves the way to the characterization of molecular interaction between Flaviviruses and Alphaviruses in mosquito in vitro and in vivo. This study can be crucial for a better understanding of disease and epidemiology during simultaneous outbreaks
Beasley, David Wayne Colin. "Identification of functional epitopes on dengue 1 environs." Thesis, Queensland University of Technology, 1999.
Find full textBrown, Jennifer L. "A Molecular and Immunological Investigation of Cellular Responses to Dengue Virus: Identification of Potentially Upregulated Host Genes and the Construction of a Vaccinia Virus Expressing the Dengue 1 Hawaii NS3 Protein." Digital WPI, 2000. https://digitalcommons.wpi.edu/etd-theses/187.
Full textMurphy, Amanda. "Eco-epidemiology of dengue and Ross River viruses across rural and urban environments." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/204283/1/Amanda_Murphy_Thesis.pdf.
Full textChoi, Wai-yee Junet, and 蔡偉儀. "Serum neopterin for early assessment of severity of severe acute respiratory syndrome and Dengue virus infection." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B32031579.
Full textChoudhury, Md Abu Hasnat Zamil. "Population Dynamics of RNA viruses." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/60866/1/Md._Choudhury_Thesis.pdf.
Full textWarke, Rajas V. "Molecular Dissection of the Cellular Reponse to Dengue Virus Infection." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/366.
Full textCollins, Jacob M. "Transcriptional Regulation of the Interleukin-8 Promoter by Multiple Dengue Viral Proteins: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/616.
Full textOstrout, Nicholas D. "Vaccinia and Dengue Viruses: Exploring Current Fundamental Issues of Memory T Cells and Utilizing Comparative Quantitative Immunology to Compare Correlates of Protection Following Smallpox Immunization." Cleveland, Ohio : Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1205938117.
Full textTricou, Vianney M. "Dengue diagnostics and therapeutic interventions in Viet Nam." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:46dfff8c-f7d2-4c43-b053-a5438531290a.
Full textMathew, Anuja. "Human T Cell Responses to Dengue Virus Infections: CD8+CTL and Acute Immunosuppression: a Dissertation." eScholarship@UMMS, 1999. https://escholarship.umassmed.edu/gsbs_diss/18.
Full textNightingale, Zachary Davis. "Distinct Behaviors of Infected and Bystander Dendritic Cells Following Exposure to Dengue Virus: A Dissertation." eScholarship@UMMS, 2007. https://escholarship.umassmed.edu/gsbs_diss/361.
Full textFriberg-Robertson, Heather L. "CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation." eScholarship@UMMS, 2010. https://escholarship.umassmed.edu/gsbs_diss/513.
Full textEkwudu, O'mezie. "Diversity of arthropod-borne viruses and implications for Wolbachia-based biocontrol." Thesis, Queensland University of Technology, 2019. https://eprints.qut.edu.au/127159/1/O%27mezie_Ekwudu_Thesis.pdf.
Full textChepkorir, Edith. "Assessing the risk of Transmission of Yellow Fever and Dengue viruses by Aedes (Stegomyia) mosquito populations in Northern Kenya." Thesis, University of Pretoria, 2019. http://hdl.handle.net/2263/75861.
Full textThesis (PhD)--University of Pretoria,2020
National Institute of Health Sciences L'oreal- UNESCO for women in science
Medical Virology
PhD in Medical Virology
Restricted
Medin, Carey L. "Chemokine Induction by Dengue Virus Infection: Mechanisms and the Role of Viral Proteins: a Dissertation." eScholarship@UMMS, 2005. https://escholarship.umassmed.edu/gsbs_diss/30.
Full textPickett, Brett E. "The contribution of different mechanisms of viral sequence variation to the evolution of positive-sense single-stranded RNA viruses." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2010. https://www.mhsl.uab.edu/dt/2010p/pickett.pdf.
Full textGirmann, Mirko [Verfasser], and Jürgen [Akademischer Betreuer] May. "Seroprevalence of Antibodies against Chikungunya, Dengue and Rift Valley Fever Viruses after Febrile Illness Outbreak, Madagascar / Mirko Girmann. Betreuer: Jürgen May." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2014. http://d-nb.info/1051435625/34.
Full textLuppino, Plinio Luis. "Detecção do vírus dengue pela técnica de aglutinação do látex modelo experimental." Faculdade de Medicina de São José do Rio Preto, 2007. http://bdtd.famerp.br/handle/tede/21.
Full textDengue is the arthropod-borne transmitted viral disease of highest worldwide prevalence in mortality and morbidity. The proportion is pandemic ranging 1.6 million of infected patients yearly. Clinical presentation associated to epidemiological factors such as dengue prevalence in the patient s origin have been the only mean for early diagnosis. Laboratorial diagnosis, the conclusive, requires several days when there is viral isolation. Serological methods depend on high level of specific antibodies, and molecular methods are not available for the majority of laboratories of diagnosis and routine. The purpose of this study was to develop an agglutination method using latex to detect dengue virus, using biological samples of mice infected with dengue 1 Mochizuki strain by intracerebral via, and anti-dengue 1 specific antibodies from immunized mice. According to the results, this method was feasible for the dengue viruses diagnosis in positive samples of experimental animals. It provides further approaches for rapid detection of dengue in susceptible populations during the first days of the disease.
A dengue é a doença viral, transmitida por artrópode, de maior prevalência mundial em morbidade e mortalidade. Alcança proporções pandêmicas, estimando-se em 1,6 milhões de doentes anualmente. Manifestações clínicas características, associadas a fatores epidemiológicos, como prevalência da dengue na região de origem do paciente, têm sido os únicos instrumentos de diagnóstico precoce. O diagnóstico laboratorial, que é definitivo, demanda vários dias, quando realizado o isolamento viral. Métodos sorológicos dependem de níveis elevados de anticorpos específicos e os métodos moleculares não estão disponíveis para a maioria dos laboratórios de diagnóstico e rotina. Este estudo teve como objetivo desenvolver método de aglutinação do látex para a detecção do vírus dengue, utilizando amostras biológicas de camundongos infectados por via intracerebral com dengue 1, cepa Mochizuki e anticorpos específicos anti-dengue 1, obtidos de camundongos imunizados. Os resultados obtidos demonstraram a viabilidade deste método para diagnóstico do vírus Dengue em amostras positivas de animais de experimentação, abrindo novas perspectivas para o diagnóstico precoce da dengue na população susceptível, durante os primeiros dias de sintomas.
Sayce, Andrew Cameron. "Iminosugars as dengue virus therapeutics : molecular mechanisms of action of a drug entering clinical trials." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:8d4da0ce-bfa6-447d-a280-630479f898af.
Full textBashyam, Hema Sundara. "Serotype Cross-Reactive CD8+ T Cell Response to Heterologous Secondary Dengue Virus Infections in Humans: a Dissertation." eScholarship@UMMS, 2006. https://escholarship.umassmed.edu/gsbs_diss/258.
Full textAlves, Beatriz Santos Capela. "Caracterização das proteinas humanas Mov34 e PACT e analise da sua interação com o RNA do virus da dengue." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317180.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-11T18:49:26Z (GMT). No. of bitstreams: 1 Alves_BeatrizSantosCapela_D.pdf: 5512305 bytes, checksum: 707ea6299bc24ddc6fb459520d79aeee (MD5) Previous issue date: 2008
Resumo: O combate à dengue atualmente está limitado praticamente aos esforços de eliminação do mosquito transmissor, o Aedes aegypti, porém esta estratégia não tem se mostrado eficiente. O desenvolvimento de novos instrumentos de combate à dengue requer, portanto, maior conhecimento sobre a biologia do vírus com relação à sua interação com seus hospedeiros. O genoma do vírus é constituído por um RNA simples-fita de polaridade positiva e possui duas regiões não traduzidas (5¿ e 3¿ UTR). A região 5¿UTR viral possui organização similar à dos mRNAs eucarióticos, diferentemente da região 3¿UTR que é longa e não possui cauda de poli(A). Em vez disso, na região 3¿UTR encontram-se estruturas conservadas entre os diferentes Flavivirus, dentre elas a estrutura 3¿ stem-loop (3¿SL) que é indispensável para a replicação do RNA viral. O objetivo do nosso estudo foi identificar novas proteínas humanas capazes de interagir com a estrutura 3¿SL do RNA do vírus da dengue. Dados da literatura descrevem que a proteína Mov34 de camundongo interage com 3¿SL do vírus da encefalite japonesa. Devido à alta similaridade entre as proteínas ortólogas humana e de camundongo, bem como das respectivas estruturas 3¿SL dos vírus da dengue e da encefalite japonesa, foi testada a interação entre a Mov34 humana com o 3¿SL do vírus da dengue. Porém, em nenhuma das condições testadas foi possível obter evidência de interação da Mov34 humana com 3¿SL dos vírus da dengue e da encefalite japonesa. Para a identificação de novas proteínas que são capazes de interagir com a estrutura 3¿SL do RNA do vírus da dengue foi utilizado o ensaio de triplo-híbrido de levedura. A proteína humana PACT, conhecida como proteína celular ativadora de PKR, foi isolada neste ensaio utilizando 3¿SL como isca. PKR é uma quinase ativada por PACT ou RNA dupla-fita. A ativação de PKR leva a um estado antiviral adquirido pela fosforilação do fator de iniciação da tradução eIF2a e conseqüente inibição da tradução. Além disso, PKR está envolvida em outras vias de transdução de sinal e na resposta celular à proteínas desenoveladas. A ação antiviral de PACT é evidenciada pela ação de proteínas dos vírus influenza A e herpes simplex tipo 1 que inibem a ativação de PKR por PACT e por RNA dupla-fita. A interação direta de PACT com 3¿SL do RNA do vírus da dengue foi confirmada por ensaio de UVcrosslinking PACT possui três domínios de interação com RNA dupla-fita, sendo que os dois domínios N-terminais são responsáveis pela sua interação com o 3¿SL. Foi identificada uma região específica do 3¿SL, o stem-loop superior, onde PACT interage com maior afinidade. Além disso, foi mostrado que PACT endógena de células HEK293 é capaz de interagir com o 3¿SL biotinilado. Para caracterizar a função desta interação durante a infecção viral, foi desenvolvida uma linhagem celular com inibição da expressão de PACT através da técnica de RNA de interferência. Com esta linhagem poderemos analisar a importância da interação entre PACT e o RNA do vírus da dengue quanto à ativação e/ou inibição de PKR durante a infecção viral
Abstract: The combat to the dengue virus is basically limited to the efforts in eliminating the transmitter mosquito, the Aedes aegypti. But this strategy is not very efficient. The development of new instruments of combat to dengue virus requires improved knowledge about the virus biology and its relation to hosts. The dengue virus genome is a single-stranded RNA of positive polarity flanked by a 5¿ untranslated region (UTR) of ~100 bases and a highly structured 3¿ UTR of ~450 bases. As many other viruses, dengue encodes the enzymes required for its genome replication, but relies completely on the host translational machinery to synthesize its proteins. The essential difference between host cellular mRNAs and dengue virus genome RNA involves the 3¿UTR, which instead of a polyadenylate tail contains highly conserved structural elements, including the 3' stem-loop (3¿SL), located at the 3' terminus of the 3'UTR of many flaviviruses that is essential for their replication. The aim of this study is to identify new human proteins capable of interacting with dengue virus RNA 3¿SL structure. Literature data describe that the murine Mov34 protein interacts with Japanese encephalitis virus 3¿SL. Giving the high similarity between the human and murine ortholog proteins, as well as the conservation of the Flavivivirus RNA 3¿SL structure, we tested the interaction between the human Mov34 and the dengue virus 3¿SL. However, no interaction was detected under the conditions used in this work. In addition, the yeast three-hybrid system was used to screen for novel proteins that interact with the dengue virus 3¿SL. Human PACT, known as the cellular protein activator of PKR, was identified as a putative 3¿SL-interacting protein. PKR is an interferon-inducible, PACT or double-stranded RNA activated protein kinase. Activated PKR phosphorylates the translation initiation factor eIF2a, inhibiting translation of cellular and viral RNAs, leading to a cellular antiviral state. PACT and doublestranded RNA activation of PKR is inhibited by influenza A and herpes simplex type 1 virus proteins during viral infection, indicating that PACT plays a role in the cellular antiviral state. Direct interaction between PACT and 3¿SL was confirmed by UV-crosslinking assays. PACT contains three doublestranded RNA interaction motifs, but only the two N-terminal motifs are responsible for 3¿SL interaction. A 3¿SL specific region, the top stem-loop, was identified to interact with PACT with higher affinity. Furthermore, HEK293 cells endogenous PACT interacts with biotin-labeled 3¿SL. To further characterize PACT-3¿SL interaction during dengue virus infection, a cell line with low expression of PACT was developed using the RNA interference technique. This cell line will be used to determine the propagation rate of dengue virus which is expected to reveal the importance of PACT either for the cell antiviral state or for dengue virus proliferation
Doutorado
Genetica Animal e Evolução
Doutor em Genetica e Biologia Molecular
Beaumier, Coreen Michele. "Cross-Reactive Memory CD4+ and CD8+ T Cells Alter the Immune Response to Heterologous Secondary Dengue Virus Infections in Mice: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/350.
Full textTiew, Kok-Chuan. "Dengue virus protease inhibitors." Thesis, Wichita State University, 2011. http://hdl.handle.net/10057/6117.
Full textThesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Chemistry.
Lindegren, Gunnel. "Optimisation of dengue diagnostic tools in order to increase the knowledge of the pathogenesis." Stockholm : Department of Microbiology, Karolinska Institutet, 2008. http://diss.kib.ki.se/2008/978-91-7409-129-8/.
Full textAravapalli, Sridhar. "Dengue virus and West Nile virus protease inhibitors." Diss., Wichita State University, 2013. http://hdl.handle.net/10057/6719.
Full textThesis (Ph.D.)--Wichita State University, Fairmount College of Liberal Arts and Sciences, Dept. of Chemistry
Tran, Tuan Anh. "Screening against the dengue virus polymerase." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4006.
Full textDengue fever, one of the most widely emerging diseases nowadays with 390 million infections each year (WHO), is caused by Dengue virus in which no official antiviral reagent or vaccine is available. The NS5 protein has an important role in the replication cycle. This protein consists of a S-adenosyl methionine transferase at N-terminal and a RNA dependent RNA polymerase (RdRp) at C-terminal. This NS5 RdRp can catalyse for not only synthesis of minus-strand RNA to be used as the template to synthesize additional plus-strand RNA but also synthesizing a complement RNA from a short RNA template without primer (de novo). In this research we present the production and activity test for NS5 protein and N-terminal extended sequence 266-900 from NS5 RdRp of all first four serotypes of Dengue virus and a construct of sequence 273-900 using a new enzymatic assay, using Picogreen as fluorescent reagent. Using this fluorescent reagent also helped determining the optimised conditions to develop a screening assay for inhibitors against dengue polymerase activity. In addition, four flavonoids, Hinokiflavone, Apigenin, Quercetin and Amentoflavone showed approximate IC50 values when testing on all NS5 and polymerase protein constructs of all four serotypes
He, Runtao. "Characterization of dengue virus envelope protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24745.pdf.
Full textSchaar, Hilde van der. "Cell entry mechanisms of dengue virus." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 2009. http://irs.ub.rug.nl/ppn/318.
Full textThu, Hlaing Myat. "Virus diversity and the emergence of Dengue." Thesis, Queensland University of Technology, 2004. https://eprints.qut.edu.au/16081/1/Hlaing_Myat_Thu_Thesis.pdf.
Full textThu, Hlaing Myat. "Virus Diversity and the Emergence of Dengue." Queensland University of Technology, 2004. http://eprints.qut.edu.au/16081/.
Full textPatramool, Sirilaksana. "Interactions virus (dengue)-vecteurs (aedes) et mise en évidence d'une méthode d'isolement des virus de la dengue et du chikungunya." Thesis, Montpellier 2, 2013. http://www.theses.fr/2013MON20139.
Full textDengue (DENV) and Chikungunya (CHIKV) viruses are two emerging arboviruses that are transmitted to humans by the bite of Aedes sp. mosquito vectors. Neither vaccines, nor medical treatments, are commercially available for these infections. It is, therefore, necessary to elaborate novel strategies to isolate the circulating viruses and block their transmission.Our understanding of the molecular mechanisms involved, during the infection of the Aedes vector by dengue virus (DENV), especially serotypes 1 and 3, remains very scant. We, therefore, performed a proteomics analysis of an Aedes albopictus cell line, infected by these two DENV serotypes, and showed that the cells use both anti-oxidant and energy-production mechanisms in the fight against the virus. These results should help to improve our knowledge of the interaction of the DENV virus and the Aedes mosquito vector, at the cellular level, with the aim of designing efficient strategies for the control of this virus. We have, in addition, developed a rapid and sensitive isolation technique, based on viral particle adsorption to magnetic beads coated with an anionic polymer. The use of this technique is of great interest, as it permits the rapid and simultaneous detection and isolation of CHIKV and DENV from samples with reduced viral loads
Hacker, Kari Ema De Silva Aravinda Manu. "Characterization of dengue virus interactions with host cells." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2009. http://dc.lib.unc.edu/u?/etd,2845.
Full textTitle from electronic title page (viewed Jun. 4, 2010). "... in partial fulfillment of the degree of Doctor of Philosophy in the Department of Microbiology and Immunology." Discipline: Microbiology and Immunology; Department/School: Medicine.
Katri, Patricia. "Modeling the Transmission Dynamics of the Dengue Virus." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_dissertations/417.
Full textWangteeraprasert, Apirath. "CD8+ T-cells responses in Dengue virus infection." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/39398.
Full textTwiddy, Sally Susanna. "The molecular epidemiology and evolution of dengue virus." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269490.
Full textXu, Yongtao. "Computational design of peptide inhibitors for dengue virus." Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603298.
Full textLin, Yu-Ying, and 林育瑩. "Dengue Viruses Inhibit Mosquito JAK-STAT Signaling." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/pbqwtx.
Full text國防醫學院
微生物及免疫學研究所
101
Dengue fever is a mosquito-borne disease endemic in many countries of the world including Taiwan. This disease is transmitted the dengue virus by Aedes aegypti and Aedes albopictus female mosquito biting to human host. These two mosquito species are prevalent in Taiwan, with Aedes aegypti present only in southern location. Mosquito does not have the adaptive immunity as mammals, only exist the innate immunity to struggle against microbe such as virus. The dengue virus is circulated between the mosquito vector and human being; it might develop some strategy to overcome the innate immunity in the mosquito vector for virus persistency and survival cycle in nature. The innate immune system or mosquito regulated by three main signaling pathways: the Toll-Dorsal/Dif, immune deficiency (IMD)-Rel, and JAK-STAT pathways for defending microbial infection. The Toll pathway is activated by fungi, Gram-positive bacteria, and viruses through pattern recognition receptor (PRRs). The Imd pathway is involves in the protection against Gram-negative. Also, activation of JAK-STAT pathway may lead to defense against viral infection. We had shown that dengue 2 virus infected C6/36 cells and dengue 2 NS3 plasmid transfected C6/36 cells with decreased STAT phosphorylation. The EMSA also showed decreased STAT binding activity in nucleus from dengue NS3 plasmid transfected C6/36 cells. In this study, we assay the JAK-STAT function of these two mosquitoes by infection with DENV-1, DENV-2, DENV-3, and DENV-4 virus in C6/36 (Aedes albopictus mosquito cells) and CCL-125 (Aedes aegypti mosquito cells). The construct 6X ST-luc plasmid contains 6XSTAT (TTCNNNGAA) binding site is for mosquito active STAT binding. The STAT activity of mosquito cells or adult female after four serotype dengue viruses infection were assayed using this 6X ST-luc plasmid and all showed decreased activity. The EMSA also showed decrease STAT binding activity after four serotype dengue virus infection in mosquito cells. All above results demonstrated that four serotype dengue viruses inhibit the JAK/STAT pathway in mosquito cell lines and adult female mosquitoes in vivo.