To see the other types of publications on this topic, follow the link: Dental caries Antibacterial agents. Nanoparticles.
Journal articles on the topic 'Dental caries Antibacterial agents. Nanoparticles'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Dental caries Antibacterial agents. Nanoparticles.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Song, Wenjing, and Shaohua Ge. "Application of Antimicrobial Nanoparticles in Dentistry." Molecules 24, no. 6 (March 15, 2019): 1033. http://dx.doi.org/10.3390/molecules24061033.
Full textAbstract:
Oral cavity incessantly encounters a plethora of microorganisms. Plaque biofilm—a major cause of caries, periodontitis and other dental diseases—is a complex community of bacteria or fungi that causes infection by protecting pathogenic microorganisms from external drug agents and escaping the host defense mechanisms. Antimicrobial nanoparticles are promising because of several advantages such as ultra-small sizes, large surface-area-to-mass ratio and special physical and chemical properties. To better summarize explorations of antimicrobial nanoparticles and provide directions for future studies, we present the following critical review. The keywords “nanoparticle,” “anti-infective or antibacterial or antimicrobial” and “dentistry” were retrieved from Pubmed, Scopus, Embase and Web of Science databases in the last five years. A total of 172 articles met the requirements were included and discussed in this review. The results show that superior antibacterial properties of nanoparticle biomaterials bring broad prospects in the oral field. This review presents the development, applications and underneath mechanisms of antibacterial nanoparticles in dentistry including restorative dentistry, endodontics, implantology, orthodontics, dental prostheses and periodontal field.
2
Idorași, Laura, Laura Cîrligeriu, Cosmin Sinescu, Cristian Zaharia, Adrian Tudor Stan, and Meda Lavinia Negruțiu. "Silver Nanotechnology — the Future in Caries Therapy? A Report of Two Cases." Journal of Interdisciplinary Medicine 2, no. 1 (March 1, 2017): 67–71. http://dx.doi.org/10.1515/jim-2017-0034.
Full textAbstract:
AbstractIntroduction: Dental decays remain the most common and rampant biofilm-dependent oral disorders. Influencing the delicate dynamic between demineralization and remineralization is a big challenge in clinical practice, and nanotechnology is considered a viable solution. The therapeutic management of caries, which includes nanotechnology, has two big approaches, an antibacterial one and a remineralizing one. Silver is recognized to display a powerful toxicity to a large variety of micro-organisms, thus silver-based composites have been widely used in several bactericidal applications.Case report: We present our attempts and results in using silver nanoparticle solutions on a 14-year-old and a 34-year-old patient with dental decays and no previous dental pain. One of the cases was treated with chlorhexidine 2% and the other with Nanocare Plus, as antimicrobial agents. In both cases we recorded decreased values of the bacterial burden in comparison with the initial values.Conclusion: This two-case presentation compared the antibacterial effect of two antibacterial solutions, providing useful information regarding novel therapies for dental caries, but further research in this domain is needed.
3
Kariminik, Ashraf, and Mohammad-Mahdi Motaghi. "Evaluation of Antimicrobial susceptibility pattern of Streptococcus mutans isolated from dental plaques to chlorhexidine, nanosil and common antibiotics." International Journal of Life Sciences 9, no. 2 (February 10, 2015): 18–21. http://dx.doi.org/10.3126/ijls.v9i2.12040.
Full textAbstract:
Streptococcus mutans is one of the most important leading causes of dental diseases worldwide and is considered as one of the main causative agent of dental caries. Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems. The aim of this study was to examine susceptibility of Streptococcus mutans isolates to some antibiotics, chlorhexidine and nanosil. The study subjects comprised of caries active individual volunteers attending the outpatient department of different dental college hospitals of Kerman. The saliva sample of 2-5 ml was collected from each individual in sterile capped bottles and were immediately transported to the laboratory and processed for the screening of S. mutans. The specific selective media, Blood agar and Tryptone yeast cysteine media agar were used for the screening and isolation of Streptococcus mutans and incubated anaerobically at 37?C for 48 hrs. Colonies of mutans streptococci were examined under a dissecting microscope and identified by their distinctive colony morphology and biochemical tests .Mutacin production of all isolates were investigated.The isolates were tested for susceptibility to some antibiotics (penicillin, gentamycin, vancomycin, cephalotin), chlorhexidine and nanosil mouthwashes by disc diffusion method. The results showed that the majority of mutacin and non mutacin-producing isolates were more sensitive to the nanosil than antibiotics and chlorhexidine. More extensive research on the use of mouthwashes containing silver nanoparticles is suggested.DOI: http://dx.doi.org/10.3126/ijls.v9i2.12040 International Journal of Life Sciences 9 (2) : 2015; 18-21
4
Pereshyvailo, O., V. Holubnycha, and V. Korniienko. "Prospects for the use of silver nanoparticles for the creation of composite dental materials with antimicrobial properties." Reports of Vinnytsia National Medical University 24, no. 1 (May 18, 2020): 125–28. http://dx.doi.org/10.31393/reports-vnmedical-2020-24(1)-24.
Full textAbstract:
Annotation. Due to the uncontrolled use of antibiotics, the issue of antibiotic resistance has become vitally important. Search the alternative antimicrobials preparation is urgent question. Despite significant achievements in dentistry, the quality of root canals endodontic treatment remains relevant issue. Enterococcus faecalis is one of the ESKAPE group pathogen as well as one of the most common causative agents of intracanal caries. Ag nanoparticles (AgNPs) have shown high efficacy against several polyresistant strains. Therefore, the purpose of our study was to evaluate the possibility of AgNPs using to create composite dental materials with antimicrobial activity. Statistical processing of the obtained results was performed using the software package for statistical data processing Graph Pud Prism 8 with the definition of the Student's criterion. The antibacterial effect of Ag NPs was evaluated against the clinical strain of E. faecalis by determining the minimum inhibitory concentration (serial dilution method) and the minimum bactericidal concentration (direct inoculation of the microorganism from liquid onto solid media). The influence of AgNPs on the multiplication of the bacterial population and on the processes of biofilm formation were investigated. The MIC and MBC were 2.5 µg / ml. Time-kill curve of E. faecalis strain indicates the need for prolonged contact of microorganisms with the nanosilver preparation and reflect a gradual decrease in the number of bacteria in the sample. AgNPs inhibit the formation of biofilms in the early stages and effectively affect 3-days biofilms. The action of the silver nanoparticles at the initiation stage reduced the total volume of the unformed biofilm by 2 times at concentrations of 10 and 20 MICs. The inhibitory effect of silver on the formed 3-day biofilm was dose-dependent and increased with increasing concentration. The obtained results show AgNPs inhibit the growth and reproduction of planktonic and film forms of E. faecalis and can be used to create dental composite with antimicrobial activity.
5
Ibrahim, Maria Salem, Isadora Martini Garcia, Taissa Vila, Abdulrahman A. Balhaddad, Fabrício Mezzomo Collares, Michael D. Weir, Hockin H. K. Xu, and Mary Anne S. Melo. "Multifunctional antibacterial dental sealants suppress biofilms derived from children at high risk of caries." Biomaterials Science 8, no. 12 (2020): 3472–84. http://dx.doi.org/10.1039/d0bm00370k.
Full text
6
Unosson, Erik, Yanling Cai, Xiyuan Jiang, Jesper Lööf, Ken Welch, and Håkan Engqvist. "Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries." International Journal of Dentistry 2012 (2012): 1–7. http://dx.doi.org/10.1155/2012/529495.
Full textAbstract:
A modified direct contact test was used to evaluate the antibacterial properties of four commercially available dental luting agents (RelyX Unicem, Ketac Cem, Ceramir Crown & Bridge and Harvard Cement) and two reference materials (glass-ionomer cement and calcium aluminate cement) compared to a negative-control material (PMMA).Streptococcus mutansbacteria were placed in direct contact with specimens that had been aged for 10 min, 1 day, and 7 days, in order to test the antibacterial properties of the materials. A metabolic assay containing resazurin was used to quantify the amount of viable bacteria remaining after the direct contact tests. The effects of pH and fluoride on bacteria proliferation were also evaluated. Strongest antibacterial properties were found for calcium aluminate cement, followed by Ceramir Crown & Bridge and RelyX Unicem. Ketac Cem, Harvard Cement, and the reference glass-ionomer cement showed bacteria content either higher than or not significantly different from the PMMA control in all instances. pH levels below 6.3 and above 9.0 were found to have negative effects on bacterial proliferation. No correlation between either acidic materials or fluoride release and antibacterial properties could be seen; rather, basic materials showed stronger antibacterial properties.
7
Melo, Mary Anne S., Lei Cheng, Michael D. Weir, Ru-Ching Hsia, Lidiany K. A. Rodrigues, and Hockin H. K. Xu. "Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles." Journal of Biomedical Materials Research Part B: Applied Biomaterials 101B, no. 4 (December 20, 2012): 620–29. http://dx.doi.org/10.1002/jbm.b.32864.
Full text
8
Butrón Téllez Girón, Claudia, Juan F. Hernández Sierra, Idania DeAlba‐Montero, María de los A. Urbano Peña, and Facundo Ruiz. "Therapeutic Use of Silver Nanoparticles in the Prevention and Arrest of Dental Caries." Bioinorganic Chemistry and Applications 2020 (August 12, 2020): 1–7. http://dx.doi.org/10.1155/2020/8882930.
Full textAbstract:
Dental caries is one of the major diseases of the oral cavity affecting humans worldwide. Different alternatives have been used for its control, but its incidence and prevalence are still high. On the other hand, silver has been used for centuries due to its antimicrobial properties. With advances in nanotechnology, the use and research in nanomaterials has increased, recently, and silver nanoparticles have become an essential part of the dental practice, giving materials physical and chemical improvements in their properties, used for their antibacterial capacity preventing and arresting dental caries. The objective of this review was to examine the use of silver nanoparticles, in the treatment of dental caries in the remineralization of teeth hard tissues, as well as the antimicrobial potential, cytotoxicity, and long-term effectiveness.
9
Ambulkar, Sonali, Vidya Tale, and Shobha Khilari. "Evaluation of the Antibacterial Potential of Traditional Medicinal Plants against Bacteria Isolated from Dental Caries." Journal of Pure and Applied Microbiology 15, no. 3 (June 26, 2021): 1204–10. http://dx.doi.org/10.22207/jpam.15.3.10.
Full textAbstract:
Dental caries are one of the leading infectious microbial diseases globally. Streptococcus species are the predominant causative agents for the formation of dental caries. Various antibiotics have been reported for the treatment of dental caries in humans. However, owing to the increasing evidence of microbial resistance, there is a need to develop safe and effective alternative treatments for infections. Traditional medicinal plants and their bioactive products have been explored worldwide for the treatment of various diseases and infections. These plants have great potential for creating novel medications without any side effects. The present study aimed to elucidate the antibacterial potential of medicinal plants against biofilm-forming bacteria from dental caries. Bacteria from dental caries were isolated and identified using 16S rRNA sequencing technique and the predominant bacterial isolates were Streptococcus mutans (MH889143), Enterococcus faecalis (MH793461), Rothia dentocariosa (MH824681), and Streptococcus anginosus (MH889145). The antibacterial potential of seventeen medicinal plants was determined against these bacterial isolates using the agar well diffusion method. The aqueous extracts of Moringa oleifera, Ficus benghalensis, Ficus racemosa, Ficus religiosa, Senegalia catechu, Pistacia integerrima, and Quercus infectoria showed significant inhibition against all bacterial isolates. Pistacia integerrima and Quercus infectoria showed the maximum inhibition. The present study confirmed that traditional medicinal plants could be helpful for the treatment of oral and dental ailments.
10
Liu, Zheng, Lihua Zhang, Jincai Wang, Yanping Li, Yiqun Chang, Xiaoling Huang, Jun Duan, Yilong Ai, Xuxin Zeng, and Jialiang Guo. "Virtual Screening and Biological Evaluation of Anti-Biofilm Agents Targeting LuxS in the Quorum Sensing System." Natural Product Communications 16, no. 6 (June 2021): 1934578X2110196. http://dx.doi.org/10.1177/1934578x211019625.
Full textAbstract:
Biofilm formation is considered as a crucial factor in various oral diseases, such as dental caries. The quorum sensing (QS) signaling system was proved to have a crucial role in the microbial dental plaque biofilm formation of Streptococcus mutans ( S. mutans). LuxS was critical to regulating the QS system and survival of the bacterium, and, therefore, compounds which target LuxS may be a potential therapy for dental caries. The binding activities of 37,170 natural compounds to LuxS were virtually screened in this study. Baicalein and paeonol were chosen for further research of the binding mode and ΔG values with LuxS. Both baicalein and paeonol inhibited the biofilm formation without influence on the growth of S. mutans. Baicalein also distinctly reduced the production of both rhamnolipids and acids. The results provide us with a new approach to combat dental caries instead of the traditional use of antibacterial chemicals.
11
Koo, H., B. Schobel, K. Scott-Anne, G. Watson, W. H. Bowen, J. A. Cury, P. L. Rosalen, and Y. K. Park. "Apigenin and tt-Farnesol with Fluoride Effects on S. mutans Biofilms and Dental Caries." Journal of Dental Research 84, no. 11 (November 2005): 1016–20. http://dx.doi.org/10.1177/154405910508401109.
Full textAbstract:
Apigenin (Api) and tt-farnesol (Far) are two naturally occurring agents that affect the development of cariogenic biofilms. Fluoride (F) interferes physicochemically with caries development and also exhibits antibacterial activity. We examined whether the association of Api and Far enhance the anti-caries properties of F by acting cooperatively on the expression of virulence of Streptococcus mutans. The biological effects of each of the agents were greatly enhanced when used in combination with F. In general, biofilms treated with Api and/or Far in combination with F displayed less biomass and fewer insoluble glucans and iodophilic polysaccharides than did those treated with the test agents alone ( P < 0.05). The combination of the test agents with F was highly effective in preventing caries development in rats, especially Api+Far+F, and results were comparable with those observed with chlorhexidine + F (positive control). Results from these studies showed that apigenin and tt-farnesol may enhance the cariostatic effectiveness of fluoride.
12
Moghadam, Parisa, Shahram Dadelahi, Yasamin S. Hajizadeh, Milad G. Matin, Milad Amini, and Saba Hajazimian. "Chemical Composition and Antibacterial Activities of Sumac Fruit (Rhus coriaria) Essential Oil on Dental Caries Pathogens." Open Microbiology Journal 14, no. 1 (June 29, 2020): 142–46. http://dx.doi.org/10.2174/1874285802014010142.
Full textAbstract:
Introduction: Dental caries is an infectious disease, and various microorganisms are involved in its progression. The common antibacterial agents against oral pathogens have many side effects and their excessive use cause drug resistance. Therefore, the identification of natural compounds and medicinal plants with antibacterial activity has been considered by the researchers. The Sumac (Rhus coriaria) is one of the native plants of Iran, which used as a food flavoring. Aims & Objectives: The aim of this study was to evaluate the antimicrobial activity of Sumac essential oil on oral pathogens, including Streptococcus mutans, Lactobacillus rhamnosus, and Actinomyces viscosus. Materials & Methods: In this study, the Sumac essential oil was prepared and its antibacterial activity was evaluated by disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) methods on established terminology strains of S. mutans, L. rhamnosus, and A. viscosus. Results: The obtained results showed that the Sumac essential oil has high inhibitory effects against S. mutans, followed by L. rhamnosus and A. viscosus. The predominant compound in the Sumac essential oil was related to Beta-caryophyllene. Conclusion: In conclusion, Sumac essential oil has an appropriate antibacterial activity and can be used in the pharmaceutical industry to produce antibacterial agents and mouthwash, against oral infectious diseases and dental caries.
13
Frolov, Georgy A., Yakov N. Karasenkov, Alexander A. Gusev, Olga V. Zakharova, Anna Yu Godymchuk, Denis V. Kuznetsov, Nadezda V. Latuta, and Valerii K. Leont'ev. "Germicidal Adhesives with Nanoparticles of Metals for Prevention of Recurrence of Caries." Nano Hybrids and Composites 13 (January 2017): 39–46. http://dx.doi.org/10.4028/www.scientific.net/nhc.13.39.
Full textAbstract:
New compositions of dental adhesives with nanoparticles of metals were developed, in order to achieve prolonged bactericidal properties. The studies were conducted on the culture of dental plaque. The observation period was 24 - 27 days. Further research was not possible due to the drying of wells with adhesives. Tantalum, aluminum, silver, vanadium, iron and copper nanoparticles were chosen as antimicrobial additives. It is shown that changing the mode of electric discharge dispersion-condensing device in a narrow range does not affect the antibacterial efficacy of the final product - the dental adhesive. It is found that tantalum nanoparticles increase the average value of shear strength in the system "filling material - adhesive - dentin" more than 40%.
14
Bowden, G. H. "The Role of Microbiology in Models of Dental Caries: Reaction Paper." Advances in Dental Research 9, no. 3 (November 1995): 255–69. http://dx.doi.org/10.1177/08959374950090031001.
Full textAbstract:
Models of the caries process have made significant contributions toward defining the roles of bacteria in caries. Microbiologists use a variety of in vitro systems to model aspects of the caries process. Also, in situ models in humans provide information on the microbiology of caries in vivo. These models do not involve the entire process leading to natural caries; consequently, the results from such studies are used to deduce the roles of bacteria in natural caries. Therefore, they can be described as Inferential Caries Models. In contrast, animal models and some clinical trials in humans involve natural caries and can be described as Complete Caries Models. Furthermore, these models are used in two distinct ways. They can be used as Exploratory Models to explore different aspects of the caries process, or as Test Models to determine the effects of anticaries agents. This dichotomy in approach to the use of caries models results in modification of the models to suit a particular role. For example, if we consider Exploratory Models, the in situ appliance in humans is superior to others for analyzing the microbiology of plaque development and demineralization in vivo. The chemostat and biofilm models are excellent for exploring factors influencing bacterial interactions. Both models can also be used as Test Models. The in situ model has been used to test the effects of fluoride on the microflora and demineralization, while the chemostat and biofilm models allow for the testing of antibacterial agents. Each model has its advantages and disadvantages and role in analysis of the caries process. Selection of the model depends on the scientific question posed and the limitations imposed by the conditions available for the study.
15
Ahalya, N., P. Dhamodhar, and A. D. Vaishnavi. "Green Synthesis, Characterization of Zinc Oxide Nanoparticles and their Incorporation into Glass Ionomer Cement for Inhibition of Streptococcus mutans." Asian Journal of Chemistry 33, no. 3 (2021): 515–20. http://dx.doi.org/10.14233/ajchem.2021.23037.
Full textAbstract:
In present study, zinc oxide nanoparticles were synthesised using Syzygium aromaticum and characterized using UV visible spectroscopy, SEM, XRD and FTIR techniques. The characteristic hexagonal structure of the ZnO nanoparticles was confirmed through XRD analysis. The UV-Visible spectrum showed a strong absorbance at 366 nm confirming the presence of ZnO and the peak at 496 cm-1 in FTIR indicated the Zn-O stretch bond. Average size of the zinc oxide nanoparticles obtained from SEM analysis was found to be 86 nm. The zinc oxide nanoparticles exhibited better antibacterial activity than clove extract, when tested against clinical isolates of Streptococcus mutans. The nanoparticles incorporated into the dental restorative material, glass ionomer cement (GIC) were tested against S. mutans and exhibited better antibacterial activity than clove extract. Glass ionomer cement (GIC) did not exhibit antibacterial activity alone, but the incorporation of ZnO nanoparticles into GIC significantly improved antibacterial activity. Hence, ZnO embedded GIC is a promising material in restorative dentistry for preventing the recurrence of dental caries.
16
Moradpoor, Hedaiat, Mohsen Safaei, Farzad Rezaei, Amin Golshah, Ladan Jamshidy, Reza Hatam, and Rawand S. Abdullah. "Optimisation of Cobalt Oxide Nanoparticles Synthesis as Bactericidal Agents." Open Access Macedonian Journal of Medical Sciences 7, no. 17 (August 30, 2019): 2757–62. http://dx.doi.org/10.3889/oamjms.2019.747.
Full textAbstract:
AIM: With the increased bacterial resistance and the resulting problems in recent years, it seems necessary to find new biocompatible compounds to confront this problem. This research was conducted to optimise the synthesis of cobalt oxide nanoparticles with the highest antibacterial activity.
METHODS: In the present study, 9 experiments were designed using the Taguchi method. The effect of three factors of cobalt nitrate, KOH and the stirring time in the synthesis of cobalt oxide nanoparticles with the highest antibacterial activity was investigated. The bactericidal effect of synthesised nanoparticles was evaluated using the colony-forming unit (CFU) and disk diffusion methods. The characteristics of nanoparticles were studied using the Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and the scanning electron microscopy (SEM).
RESULTS: The results indicated that all three evaluated factors were effective on the antibacterial properties of the synthesised nanoparticles. The best antibacterial activity of cobalt oxide nanoparticles was observed in experiment 9 (cobalt nitrate 0.6 M, KOH 2M and stirring time 60 min). The study of nanoparticles synthesised by FTIR, XRD, and SEM confirmed the formation of cobalt oxide nanoparticles with size (24 nm) and a proper structure (spinel structure).
CONCLUSION: Due to the optimal antibacterial properties of the synthesised cobalt oxide nanoparticles, they can be used in the fabrication of dental and medical equipment with antibacterial properties.
17
Zhou, Wen, Shiyu Liu, Xuedong Zhou, Matthias Hannig, Stefan Rupf, Jin Feng, Xian Peng, and Lei Cheng. "Modifying Adhesive Materials to Improve the Longevity of Resinous Restorations." International Journal of Molecular Sciences 20, no. 3 (February 8, 2019): 723. http://dx.doi.org/10.3390/ijms20030723.
Full textAbstract:
Dental caries is a common disease on a global scale. Resin composites are the most popular materials to restore caries by bonding to tooth tissues via adhesives. However, multiple factors, such as microleakage and recurrent caries, impair the durability of resinous restorations. Various innovative methods have been applied to develop adhesives with particular functions to tackle these problems, such as incorporating matrix metalloproteinase inhibitors, antibacterial or remineralizing agents into bonding systems, as well as improving the mechanical/chemical properties of adhesives, even combining these methods. This review will sum up the latest achievements in this field.
18
Mittapally, Sirisha, Ruheena Taranum, and Sumaiya Parveen. "Metal ions as antibacterial agents." Journal of Drug Delivery and Therapeutics 8, no. 6-s (December 15, 2018): 411–19. http://dx.doi.org/10.22270/jddt.v8i6-s.2063.
Full textAbstract:
Metals like mercury, arsenic, copper and silver have been used in various forms as antimicrobials for thousands of years. The use of metals in treatment was mentioned in Ebers Papyrus (1500BC); i.e, copper to decrease inflammation & iron to overcome anemia. Copper has been registered at the U.S. Environmental Protection Agency as the earliest solid antimicrobial material. Copper is used for the treatment of different E. coli, MRSA, Pseudomonas infections. Advantage of use of silver is it has low toxicity to human’s cells than bacteria.It is less susceptible to gram +ve bacteria than gram –bacteria due to its thicker cell wall. Zinc is found to be active against Streptococcus pneumonia, Campylobacter jejuni. Silver & zinc act against vibrio cholera & enterotoxic E. coli. The use of metals as antibacterial got reduce with discovery of antibiotics in twentieth century, immediately after that antibiotic resistance was seen due to transfer of antibiotic resistance genes by plasmids also known as Resistance Transfer Factors or R-factors. Metal complexes are used to show synergistic activity against bacteria’s like copper & chlorhexidine on dental plaque bacteria, silver nanoparticles & cephalexin against E. coli & S. aureus.
Keywords: Metals, Oligodynamic effect, Copper, Silver
19
Angel Villegas, Natalia, M. Jazmin Silvero Compagnucci, Martin Sainz Ajá, Diamela María Rocca, María Cecilia Becerra, Gustavo Fabián Molina, and Santiago Daniel Palma. "Novel Antibacterial Resin-Based Filling Material Containing Nanoparticles for the Potential One-Step Treatment of Caries." Journal of Healthcare Engineering 2019 (March 13, 2019): 1–8. http://dx.doi.org/10.1155/2019/6367919.
Full textAbstract:
The aim of this work was to study the application of resin filling containing nanomaterials for the potential treatment of caries. Zinc nanoparticles (ZnO@NP, 50 nm) were chosen for their antimicrobial capacity against aerobic bacteria, and here, they have proved to be bactericidal against anaerobic bacterial strains (Streptococcus mutans, Streptococcus mitis, and Lactobacillus spp.). Potential mechanism of action is proposed based on microbiological assays and seems to be independent of oxidative stress because the nanoparticles are effective in microaerophilic conditions. The loading of nanoparticles on the demineralized dental surface and their infiltration power were significantly improved when ZnO@NP were carried by the resin. Overall, this material seems to have a high potential to become a one-step treatment for caries lesions.
20
G, Ramya, and Arvina Rajasekar. "Enhanced Antibacterial Effect of Titanium Dioxide Nanoparticles Mediated Grape Seed Extract on Oral Pathogens - Streptococcus mutans and Lactobacillus." Journal of Evolution of Medical and Dental Sciences 10, no. 22 (May 31, 2021): 1656–61. http://dx.doi.org/10.14260/jemds/2021/344.
Full textAbstract:
BACKGROUND Streptococcus mutans is the primary pathogen responsible for initiating dental caries and decay. Lactobacilli present in caries lesions is a major contributor to caries progression. Titanium dioxide nanoparticles have been found to have a large spectrum of activity against microorganisms. The grape seed extract has been shown to have inhibitory effects against several clinically important microbes. This study was conducted to assess the enhanced antibacterial effect of titanium dioxide nanoparticles mediated grape seed extract against oral pathogens, S. mutans and lactobacillus. METHODS This experimental study was done by preparing the grape seed extract, synthesising titanium dioxide (TiO2) nanoparticles, preparing the nanoparticles powder, and then evaluating the antibacterial activity of nanoparticles against lactobacillus species and Streptococcus mutans. RESULTS The peak found in the spectroscopy indicates the formation of titanium dioxide nanoparticles. The zone of inhibition for antibiotics enhanced with 50 μl, 100 μl, 150 μl of grape seed mediated TiO2 nanoparticles were 16 mm, 18 mm and 19 mm in Streptococcus mutans. In lactobacillus species the zone of inhibition for standard antibiotics enhanced with 50 μl, 100 μl, 150 μl of grape seed mediated TiO2 nanoparticles remained constant at 35 mm. CONCLUSIONS The titanium dioxide nanoparticle mediated grape-seed extract showed excellent antimicrobial activity against lactobacillus and Streptococcus mutans. This proves that in future it can be used for treating these bacterial infections in the oral cavity after further in vivo studies. KEY WORDS Nanotechnology, Titanium Dioxide Nanoparticles, Grape Seed Extract, Lactobacillus, Enhanced Antibacterial, S. mutans
21
Zhang, Ke, Fang Li, Satoshi Imazato, Lei Cheng, Huaibing Liu, Dwayne D. Arola, Yuxing Bai, and Hockin H. K. Xu. "Dual antibacterial agents of nano-silver and 12-methacryloyloxydodecylpyridinium bromide in dental adhesive to inhibit caries." Journal of Biomedical Materials Research Part B: Applied Biomaterials 101B, no. 6 (March 26, 2013): 929–38. http://dx.doi.org/10.1002/jbm.b.32898.
Full text
22
Cheng, Lei, Jiyao Li, Libang He, and Xuedong Zhou. "Natural Products and Caries Prevention." Caries Research 49, Suppl. 1 (2015): 38–45. http://dx.doi.org/10.1159/000377734.
Full textAbstract:
Dental caries is considered as the most common polymicrobial oral disease in the world. With the aim of developing alternative approaches to reduce or prevent the decay, numerous papers showed the potential anticaries activity of a number of natural products. The natural products with anticaries effects are selected from e.g. food, beverages, flowers or traditional herbs. Most of the effective components are proven to be polyphenol compounds. Many of the natural products are studied as antibacterial agents, while some of them are found to be effective in shifting the de-/remineralization balance. However, the mechanisms of the anticaries effects are still unclear for most of the natural products. In the future, more efforts need to be made to seek novel effective natural products via in vitro experiment, animal study and in situ investigations, as well as to enhance their anticaries effects with the help of novel technology like nanotechnology.
23
Gautam, Gorika, Diksha Jha, Shailendra S. Gaurav, Ashwani K. Sharma, Pradeep Kumar, and Hemant K. Gautam. "Synthesis of carbon nanoparticles from mustard oil and evaluation of their antibacterial activity against dental caries." Micro & Nano Letters 12, no. 10 (October 2017): 799–802. http://dx.doi.org/10.1049/mnl.2017.0293.
Full text
24
Ibrahim, Maria, Faisal AlQarni, Yousif Al-Dulaijan, Michael Weir, Thomas Oates, Hockin Xu, and Mary Melo. "Tuning Nano-Amorphous Calcium Phosphate Content in Novel Rechargeable Antibacterial Dental Sealant." Materials 11, no. 9 (August 27, 2018): 1544. http://dx.doi.org/10.3390/ma11091544.
Full textAbstract:
Dental sealants with antibacterial and remineralizing properties are promising for caries prevention among children and adolescents. The application of nanotechnology and polymer development have enabled nanoparticles of amorphous calcium phosphate (NACP) and dimethylaminohexadecyl methacrylate (DMAHDM) to emerge as anti-caries strategies via resin-based dental materials. Our objectives in this study were to (1) incorporate different mass fractions of NACP into a parental rechargeable and antibacterial sealant; (2) investigate the effects on mechanical performance, and (3) assess how the variations in NACP concentration would affect the calcium (Ca) and phosphate (PO4) ion release and re-chargeability over time. NACP were synthesized using a spray-drying technique and incorporated at mass fractions of 0, 10, 20 and 30%. Flexural strength, flexural modulus, and flowability were assessed for mechanical and physical performance. Ca and PO4 ion release were measured over 70 days, and three ion recharging cycles were performed for re-chargeability. The impact of the loading percentage of NACP upon the sealant’s performance was evaluated, and the optimized formulation was eventually selected. The experimental sealant at 20% NACP had flexural strength and flexural modulus of 79.5 ± 8.4 MPa and 4.2 ± 0.4 GPa, respectively, while the flexural strength and flexural modulus of a commercial sealant control were 70.7 ± 5.5 MPa (p > 0.05) and 3.3 ± 0.5 GPa (p < 0.05), respectively. A significant reduction in flow was observed in the experimental sealant at 30% NACP (p < 0.05). Increasing the NACP mass fraction increased the ion release. The sealant formulation with NACP at 20% displayed desirable mechanical performance and ideal flow and handling properties, and also showed high levels of long-term Ca and PO4 ion release and excellent recharge capabilities. The findings provide fundamental data for the development of a new generation of antibacterial and rechargeable Ca and PO4 dental sealants to promote remineralization and inhibit caries.
25
Geetha RV, John Rozar Raj B, and Lakshmi Thangavelu. "Anti-bacterial activity of three essential oils - An in vitro study." International Journal of Research in Pharmaceutical Sciences 10, no. 2 (April 14, 2019): 1049–53. http://dx.doi.org/10.26452/ijrps.v10i2.380.
Full textAbstract:
To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.
26
Ben-Zaken, Hadar, Reut Kraitman, Shunit Coppenhagen-Glazer, Leron Khalifa, Sivan Alkalay-Oren, Daniel Gelman, Gilad Ben-Gal, Nurit Beyth, and Ronen Hazan. "Isolation and Characterization of Streptococcus mutans Phage as a Possible Treatment Agent for Caries." Viruses 13, no. 5 (May 2, 2021): 825. http://dx.doi.org/10.3390/v13050825.
Full textAbstract:
Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.
27
Gelmboldt, V. O., and V. Yu Anisimov. "Ammonium hexafluorosilicates: a new type of anti-caries agents." Farmatsevtychnyi zhurnal, no. 5-6 (February 12, 2019): 48–69. http://dx.doi.org/10.32352/0367-3057.5-6.18.04.
Full textAbstract:
It is known that dental caries is one of the most common diseases; for children, this pathology ranks first among chronic diseases and has the character of a pandemic. In the modern arsenal of means for the treatment and prevention of caries, fluoride preparations – sodium, potassium fluoride, tin difluoride, sodium monofluoridephosphate, diammine silver fluoride – occupy the leading positions. In recent decades, ammonium hexafluorosilicate and hexafluorosilicates of organic ammonium cations, which have certain advantages over traditional fluoride preparations, have been actively studied as promising anti-caries and hypo-sensitive agents.
The aim of this review is to systematize and analyze literature data, reflecting existing experience in the field of synthesis methods, studying the structure, physicochemical properties and biological activity of ammonium hexafluorosilicates as potential anti-caries agents.
The general method for the synthesis of ammonium hexafluorosilicates is the interaction of the corresponding base, or its salt, in methanol, ethanol or another solvent with stoichiometric or excess amount of hydrofluoric acid. The structure of ammonium hexafluorosilicates, which are typical supramolecular compounds, is described. Using the example of pyridinium hexafluorosilicates, the relationship between water solubility and structural characteristics of salts was demonstrated. Identification methods have been proposed for the combination of cetylpyridinium hexafluorosilicate. The results of determination of acute toxicity for a number of hexafluorosilicates in animal experiments are given. Studies of the antibacterial activity of hexafluorosilicates are described. It is shown that hexafluorosilicates significantly reduce the number of carious lesions and their depth, and significantly exceed sodium fluoride in all indicators. The feasibility of their use for the prevention of periodontitis is demonstrated. A technological scheme for obtaining a gel containing cetylpyridinium hexafluoro silicate has been proposed.
General methods for the synthesis of ammonium hexafluorosilicates include the interaction of an organic base or the corresponding hydrochloride in a solvent medium with an excess of hydrofluoric acid silica. In the crystal structures of hexafluorosilicates, hydrogen bond systems with the participation of fluorine ligands and H-donor fragments of cations perform the main structure-forming and stabilizing function. The solubility of hexafluorosilicates in water varies widely, which is determined by the nature of the ammonium cation and, in the case of pyridinium salts, is antibatically correlated with the number of strong and medium H-bonds in the salt structure. The degree of hydrolysis of hexafluoro silicates in 1×10–4 М aqueous solutions is high and in some cases reaches practically quantitative values. The results of the determination of the acute toxicity of ammonium hexafluorosilicates make it possible to classify these compounds as moderately toxic substances. All studied ammonium hexafluorosilicates exhibit high caries preventive activity, significantly reducing the number and depth of carious lesions, while simultaneously significantly improving the biochemical parameters of dental pulp and periodontal bone tissue. Ammonium hexafluorosilicates can be used as new effective anti-caries and hypo-sensitive agents.
28
Carrouel, Florence, Stephane Viennot, Livia Ottolenghi, Cedric Gaillard, and Denis Bourgeois. "Nanoparticles as Anti-Microbial, Anti-Inflammatory, and Remineralizing Agents in Oral Care Cosmetics: A Review of the Current Situation." Nanomaterials 10, no. 1 (January 13, 2020): 140. http://dx.doi.org/10.3390/nano10010140.
Full textAbstract:
Many investigations have pointed out widespread use of medical nanosystems in various domains of dentistry such as prevention, prognosis, care, tissue regeneration, and restoration. The progress of oral medicine nanosystems for individual prophylaxis is significant for ensuring bacterial symbiosis and high-quality oral health. Nanomaterials in oral cosmetics are used in toothpaste and other mouthwash to improve oral healthcare performance. These processes cover nanoparticles and nanoparticle-based materials, especially domains of application related to biofilm management in cariology and periodontology. Likewise, nanoparticles have been integrated in diverse cosmetic produces for the care of enamel remineralization and dental hypersensitivity. This review summarizes the indications and applications of several widely employed nanoparticles in oral cosmetics, and describes the potential clinical implementation of nanoparticles as anti-microbial, anti-inflammatory, and remineralizing agents in the prevention of dental caries, hypersensitivity, and periodontitis.
29
Liu, Fang, Cheng Qian, Li Xu, Rongxiu Zhang, Liang Liu, Zhigang Wu, Kai Zhang, Shengkai Liao, and Xiaofei Xie. "Analysis on the Performance of Nano-Composite Resin Dental Restorative Materials." Science of Advanced Materials 13, no. 5 (May 1, 2021): 927–37. http://dx.doi.org/10.1166/sam.2021.3995.
Full textAbstract:
After long-term use of resin materials for dental repair, plaque is easy to accumulate on the surface to form biofilms, which can cause caries. Although antibacterial agents are applied to oral resins, the antibacterial ability, mechanical performance, and biocompatibility of the material have to be considered. In this study, the cationic polymer NPVP with different lengths of alkyl chain is adopted to modify the carbon–nanodiamond (CND), and hydroxyethyl methacrylate (HEMA) is introduced to reduce the cytotoxicity of the modified particles and improve the compatibility with the resin matrix. Among the multiple composite samples obtained, MND-H10 shows the best de-aggregation effect, with a particle size of 70 nm. In addition, it plays a good antibacterial effect against Gram-negative bacteria (GNB) Escherichia coli (E. coli) and Gram-positive bacteria (GPB) Staphylococcus aureus (S. aureus). On this basis, the polymerizable antibacterial nano-composite particles (modified nano-diamonds, MNDs) are prepared and polymerized with the resin matrix Bisphenol A bis-methyl/glycidyl acrylate (Bis-GMA)/Triglycol dimethacrylate (TEGDMA) to construct an antibacterial dental repair material. After testing, the Vickers hardness, flexural strength, and flexural modulus of the 0.6 wt% modified resin material all increase by more than 45%. Due to the addition of nano-particles, the material shows a certain antibacterial ability, an increased hydrophilicity, and antibacterial adhesion ability. 10 patients with clinically defective or missing dentition during June 2017 to October 2017 are selected from the affiliated hospitals of the college, who are treated with the threaded implants. After a year of clinical observation, the threaded implants have achieved better osseointegration results.
30
Thaweboon, Boonyanit, and Sroisiri Thaweboon. "Formation of Cariogenic Bacterial Biofilm on Vanillin-Incorporated Resin-Based Dental Sealant." Key Engineering Materials 889 (June 16, 2021): 107–11. http://dx.doi.org/10.4028/www.scientific.net/kem.889.107.
Full textAbstract:
Tooth decay or dental caries is an important oral health problem involving people of all age groups. The disease is the outcome of the demineralize process in which aciduric and acidogenic bacteria in a biofilm decompose tooth structure. Dental sealant, a resin material, which is applied on the occlusal pit and fissure surfaces of the teeth as a protective layer has been commonly used to prevent dental caries. However, the microbial effect on food residue is found to be a major cause of microleakage of sealant and secondary caries. Several types of antimicrobial agents were introduced to increase the caries preventive effect of dental sealants. Vanillin, the main component of flavoring agent vanilla, has been found to have antimicrobial property against Gram-positive and Gram-negative bacteria. The objective of this study was to investigate the antimicrobial effect of vanillin-incorporated dental sealant against biofilm formation of cariogenic bacteria. Dental sealant resin samples (Clinpro; 3M ESPE, USA) were prepared in 96-well plate in accordance with the amount of vanillin adding (0%, 0.5%, 1% and 5% vanillin). The cariogenic bacterial suspensions of Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 334 were added to saliva-coated samples and incubated at 37°C in 5% CO2 atmosphere for 48 h to allow the biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. One-way ANOVA and Tukey’s test were applied to the statistical analysis. A significant inhibitory effect against L. casei biofilm was observed in all vanillin incorporated samples (0.5%, 1% and 5% vanillin) compared with samples without vanillin. The percentage of biofilm reduction was 32-39%. For S. mutans, the suppressive effect was noticed only at >1% vanillin with 18-25% biofilm reduction. In conclusion, the incorporation of vanillin to dental sealants could decrease biofilm formation of cariogenic bacteria (S. mutans and L. casei). The use of dental sealants containing vanillin could be a promising measure to prevent dental caries due to their antibacterial biofilm formation property.
31
Zegan, Georgeta, Elena Mihaela Carausu, Loredana Golovcencu, Alina Sodor Botezatu, Eduard Radu Cernei, and Daniela Anistoroaei. "Nanoparticles Deposition on Mini-implants for Osseo-integration and Antibacterial Properties Improvement." Revista de Chimie 68, no. 12 (January 15, 2018): 2929–31. http://dx.doi.org/10.37358/rc.17.12.6009.
Full textAbstract:
In last few years, researchers focused on improvement of lifespan of dental mini-implants by coating them with nanopoarticles for a better osseo-integration, which also possesses antibacterial properties. Once their surface is coated with nanoparticles type silver dopped hydroxyapatite makes them durable and safe by a good integration into tissue and by preventing bacterial infections. It is well known that a particular problem with medical devices such as dental mini-implants is bacterial colonization. For this reason the use of nanoparticles coated mini-implants is a measure to prevent or to treat infections caused by microbial agents present in oral cavity. Our study considers the possibility of using silver doped hydroxyapatite thin films on titanium mini-implants as an osseo-integration and antibacterial component. Therefore several physic-chemical analyses were performed in order to determine their properties and to open new perspectives to develop nanoparticulated thin films as coatings for orthodontic mini-implants.
32
Abdulrahman Salman, Hamzah, Senthilkumar R, and Vasundhara M. "Lack of Antibacterial Activity of Capsicum Annuum and Simarouba Glauca Against Streptococcus Mutans and Streptococcus Sobrinus." Biosciences, Biotechnology Research Asia 15, no. 2 (May 8, 2018): 311–15. http://dx.doi.org/10.13005/bbra/2634.
Full textAbstract:
Capsicum annuum L. is an edible vegetable crop while Simarouba glauca L. is a medicinal plant. The study was taken up to evaluate the antibacterial activity of these extracted plant materials against Streptococcus mutans and Streptococcus sobrinus, the main dental caries agents. Three plant materials (fruit, seeds and leaves) of Capsicum annuum L. var. baydagi dabba and two plant materials (leaves and bark) of Simarouba glauca L. were collected in the month of November and February, respectively. The plant materials were cleaned, dried and prepared for extraction. The antibacterial susceptibility testing was performed using disc diffusion method. At maximum concentration of 100 mg/ml, it was observed that both the extracted plant materials did not possess antibacterial activity against S. mutans and S. sobrinus. The study revealed the lack of antibacterial activity of C. annuum and S. glauca against S. mutans and S. sobrinus.
33
Kuang, Xinyi, Vivian Chen, and Xin Xu. "Novel Approaches to the Control of Oral Microbial Biofilms." BioMed Research International 2018 (December 31, 2018): 1–13. http://dx.doi.org/10.1155/2018/6498932.
Full textAbstract:
Effective management of biofilm-related oral infectious diseases is a global challenge. Oral biofilm presents increased resistance to antimicrobial agents and elevated virulence compared with planktonic bacteria. Antimicrobial agents, such as chlorhexidine, have proven effective in the disruption/inhibition of oral biofilm. However, the challenge of precisely and continuously eliminating the specific pathogens without disturbing the microbial ecology still exists, which is a major factor in determining the virulence of a multispecies microbial consortium and the consequent development of oral infectious diseases. Therefore, several novel approaches are being developed to inhibit biofilm virulence without necessarily inducing microbial dysbiosis of the oral cavity. Nanoparticles, such as pH-responsive enzyme-mimic nanoparticles, have been developed to specifically target the acidic niches within the oral biofilm where tooth demineralization readily occurs, in effect controlling dental caries. Quaternary ammonium salts (QAS) such as dimethylaminododecyl methacrylate (DMADDM), when incorporated into dental adhesives or resin composite, have also shown excellent and durable antimicrobial activity and thus could effectively inhibit the occurrence of secondary caries. In addition, custom-designed small molecules, natural products and their derivatives, as well as basic amino acids such as arginine, have demonstrated ecological effects by modulating the virulence of the oral biofilm without universally killing the commensal bacteria, indicating a promising approach to the management of oral infectious diseases such as dental caries and periodontal diseases. This article aims to introduce these novel approaches that have shown potential in the control of oral biofilm. These methods may be utilized in the near future to effectively promote the clinical management of oral infectious diseases and thus benefit oral health.
34
Wei, Hanqi, Zhipeng Xie, Xiuchuan Tan, Ran Guo, Yanting Song, Xi Xie, Rong Wang, Lushuang Li, Manchuriga Wang, and Yingxia Zhang. "Temporin-Like Peptides Show Antimicrobial and Anti-Biofilm Activities against Streptococcus mutans with Reduced Hemolysis." Molecules 25, no. 23 (December 4, 2020): 5724. http://dx.doi.org/10.3390/molecules25235724.
Full textAbstract:
In our previous study, temporin-GHaR (GHaR) showed potent antimicrobial activity with strong hemolytic toxicity. To overcome its weakness, we designed GHaR6R, GHaR7R, GHaR8R, GHaR9R, and GHaR9W by changing the number of positive charges and the hydrophobic surface of GHaR. With the exception of GHaR7R, the hemolytic toxicity of the derived peptides had been reduced, and the antimicrobial activities remained close to the parent peptide (except for GHaR9R). GHaR6R, GHaR7R, GHaR8R, and GHaR9W exhibited a great bactericidal effect on Streptococcus mutans (S. mutans), which is one of the main pathogens causing dental caries. According to the membrane permeation and scanning electron microscope (SEM) analysis, these derived peptides targeted to the cell membranes of planktonic bacteria, contributing to the disruption of the membrane integrity and leakage of the intracellular contents. Moreover, they inhibited the formation of biofilms and eradicated the mature biofilms of S. mutans. Compared with GHaR7R, the derived peptides showed less cytotoxicity to human oral epithelial cells (HOECs). The derived peptides are expected to be the molecular templates for designing antibacterial agents to prevent dental caries.
35
Kuang, Xinyi, Tao Yang, Chenzi Zhang, Xian Peng, Yuan Ju, Chungen Li, Xuedong Zhou, Youfu Luo, and Xin Xu. "Repurposing Napabucasin as an Antimicrobial Agent against Oral Streptococcal Biofilms." BioMed Research International 2020 (November 20, 2020): 1–9. http://dx.doi.org/10.1155/2020/8379526.
Full textAbstract:
Objectives. Disruption of microbial biofilms is an effective way to control dental caries. Drug resistance and side effects of the existing antimicrobials necessitate the development of novel antibacterial agents. The current study was aimed at investigating the antibacterial activities of the repurposed natural compound napabucasin against oral streptococci. Methods. The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibition concentration, and minimum biofilm reduction concentration of Streptococcus mutans, Streptococcus gordonii, and Streptococcus sanguinis were examined by a microdilution method. Cytotoxicity of napabucasin against human oral keratinocytes, human gingival epithelia, and macrophage RAW264.7 was evaluated by CCK8 assays. The dead/live bacterium and exopolysaccharide in the napabucasin-treated multispecies biofilms were evaluated by confocal laser scanning microscopy. Microbial composition within the napabucasin-treated biofilms was further visualized by fluorescent in situ hybridization and qPCR. And the cariogenicity of napabucasin-treated biofilms was evaluated by transverse microradiography. Results. Napabucasin exhibited good antimicrobial activity against oral streptococcal planktonic cultures and biofilms but with lessened cytotoxicity as compared to chlorhexidine. Napabucasin reduced the cariogenic S. mutans and increased the proportion of the commensal S. gordonii in the multispecies biofilms. More importantly, napabucasin significantly reduced the demineralization capability of biofilms on tooth enamels. Conclusion. Napabucasin shows lessened cytotoxicity and comparable antimicrobial effects to chlorhexidine. Repurposing napabucasin may represent a promising adjuvant for the management of dental caries.
36
Hetrodt, Franziska, Julian Lausch, Hendrik Meyer-Lueckel, Georg Conrads, and Christian Apel. "Evaluation of Restorative Materials Containing Preventive Additives in a Secondary Caries Model in vitro." Caries Research 53, no. 4 (2019): 447–56. http://dx.doi.org/10.1159/000496401.
Full textAbstract:
The incorporation of antibacterial agents into dental restorative materials is a promising strategy for secondary caries prevention. Previously, Carolacton affected Streptococcus mutans biofilm formation on composite materials in vitro. The present study evaluated secondary caries formation adjacent to Carolacton-containing composites and conventional restorative materials using an artificial biofilm model. Standardized cavities were prepared in bovine dentin-enamel samples (n = 175) and restored with various dental materials (Tetric EvoCeram [T], GrandioSo composite without [G] and with Carolacton [GC], Grandio Flow without [F] and with Carolacton [FC], GrandioSo containing sodium fluoride [GNaF], and Ketac Fil [K]). After artificial aging, S. mutans was grown on the samples for 7 days. The investigation of gap sizes and secondary caries formation was performed using confocal laser scanning microscopy and transversal microradiography. Median gap size in enamel was 9.4 µm (interquartile range 7.9–12.7). Compared to all other groups significant differences in gap sizes could be observed for Ketac Fil (p < 0.001; Mann-Whitney test). Only GrandioSo composite containing 30% sodium fluoride and Ketac Fil showed significantly smaller lesion areas in enamel (p < 0.001; Mann-Whitney test) than all other groups which was confirmed by the mineral loss data (p < 0.001; Mann-Whitney test). Based on the present in vitro results, it seems that Carolacton-containing composite in the current formulation within the shown simplified monoculture biofilm model is not able to prevent caries formation compared to fluoride-releasing restorative materials.
37
Devyatkina, N. M., N. O. Bobrova, and E. M. Vazhnichaya. "THE EFFECT OF ESSENTIAL OILS ON MICROORGANISMS CAUSING CARIES AND PERIODONTITIS." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 20, no. 2 (July 6, 2020): 253–59. http://dx.doi.org/10.31718/2077-1096.20.2.253.
Full textAbstract:
The oral cavity contains a large number of bacteria, some of which are involved in the development of caries and periodontitis (S. mutans, S. sobrinus, Lactobacilli spp, P. intermedia, P. gingivalis, and T. forythus). The disadvantages of existing antiseptics used in dentistry necessitate the study of antibacterial properties of herbal medicines, and, in particular, of essential oils. The aim of this review is to provide the analysis of literature sources from PubMed and Google Scholar databases related to the effects of essential oils of cloves, mint, thyme, eucalyptus, tea tree and their components on cariogenic and periodontopathic microflora. It was found out that the most in vitro studies evaluated the effects of essential oils or isolated compounds (eugenol, menthol, thymol, carvacrol, eucalyptol, and terpinene-4-ol) on S. mutans, which is considered to be the most cariogenic of oral streptococci, and the researchers limited to defining the susceptibility of the microorganism and effects on biofilm formation. Only in a few studies, the effects of essential oils on the virulence factors of oral pathogens, in particular glycosyl transferase, are represented. Clinical trials of essential oils, their components and combinations confirm the therapeutic potential of these agents in vivo, but raise the question of their effectiveness, taking into account the short-term action, which does not exceed the potency of chlorhexidine. Essential oils of cloves, mint, thyme, eucalyptus, tea tree and their components should be used for treating caries and periodontitis. They are also promising when used as agents of the oral care products, preservatives of the dental medicinal forms, and as remedies for halitosis. With a rational prescription, essential oils can be useful in improving the quality of dental treatment and preventive procedures.
38
Palombo, Enzo A. "Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–15. http://dx.doi.org/10.1093/ecam/nep067.
Full textAbstract:
Oral diseases are major health problems with dental caries and periodontal diseases among the most important preventable global infectious diseases. Oral health influences the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. The association between oral diseases and the oral microbiota is well established. Of the more than 750 species of bacteria that inhabit the oral cavity, a number are implicated in oral diseases. The development of dental caries involves acidogenic and aciduric Gram-positive bacteria (mutans streptococci, lactobacilli and actinomycetes). Periodontal diseases have been linked to anaerobic Gram-negative bacteria (Porphyromonas gingivalis,Actinobacillus,PrevotellaandFusobacterium). Given the incidence of oral disease, increased resistance by bacteria to antibiotics, adverse affects of some antibacterial agents currently used in dentistry and financial considerations in developing countries, there is a need for alternative prevention and treatment options that are safe, effective and economical. While several agents are commercially available, these chemicals can alter oral microbiota and have undesirable side-effects such as vomiting, diarrhea and tooth staining. Hence, the search for alternative products continues and natural phytochemicals isolated from plants used as traditional medicines are considered as good alternatives. In this review, plant extracts or phytochemicals that inhibit the growth of oral pathogens, reduce the development of biofilms and dental plaque, influence the adhesion of bacteria to surfaces and reduce the symptoms of oral diseases will be discussed further. Clinical studies that have investigated the safety and efficacy of such plant-derived medicines will also be described.
39
Talapko, Jasminka, Tatjana Matijević, Martina Juzbašić, Arlen Antolović-Požgain, and Ivana Škrlec. "Antibacterial Activity of Silver and Its Application in Dentistry, Cardiology and Dermatology." Microorganisms 8, no. 9 (September 11, 2020): 1400. http://dx.doi.org/10.3390/microorganisms8091400.
Full textAbstract:
The problem of antimicrobial resistance is increasingly present and requires the discovery of new antimicrobial agents. Although the healing features of silver have been recognized since ancient times, silver has not been used due to newly discovered antibiotics. Thanks to technology development, a significant step forward has been made in silver nanoparticles research. Nowadays, silver nanoparticles are a frequent target of researchers to find new and better drugs. Namely, there is a need for silver nanoparticles as alternative antibacterial nanobiotics. Silver nanoparticles (AgNPs), depending on their size and shape, also have different antimicrobial activity. In addition to their apparent antibacterial activity, AgNPs can serve as drug delivery systems and have anti-thrombogenic, anti-platelet, and anti-hypertensive properties. Today they are increasingly used in clinical medicine and dental medicine. This paper presents silver antimicrobial activity and its use in dentistry, cardiology, and dermatology, where it has an extensive range of effects.
40
Shilpa Merlyn Jose, Hannah.R, and Rajeshkumar S. "Preparation of zinc oxide nanoparticles using punica granatum and elettaria cardamomum fruit extract and determining its antibacterial activity against lactobacillus." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (July 8, 2020): 3372–76. http://dx.doi.org/10.26452/ijrps.v11i3.2473.
Full textAbstract:
To determine the antibacterial activity of Zinc oxide nanoparticles synthesised using Punica granatum and Elettaria cardamomum fruit extract against Lactobacillus. Punica granatum and Elettaria cardamomum have been known for their antimicrobial, antioxidant and anti-inflammatory activity. The phytochemicals present in these fruits have experimented for the preparation of various metal and metal oxide nanoparticles. Zinc oxide is a widely used metal oxide nanoparticle known for its good antimicrobial activity against a host of microbes. The current study was conducted to determine its effect against Lactobacillus, a bacteria known for its role in the progression of dental caries. Preparation of fruit extract mediated zinc oxide nanoparticles. Determining the characteristics of the nanoparticles using UV spectroscopy and SEM. Analysing the activity of these nanoparticles against Lactobacillus using agar well diffusion method. The zone of inhibition increased if the concentration of the fruit mediated zinc oxide nanoparticles increased. But it was incomparable to the standards. Hence, further studies need to be conducted using different concentration of Punica granatum and Elettaria cardamomum to determine the optimum fruit extract required for the preparation of the nanoparticles. The resultant nanoparticles can be used as an effective antimicrobial agent against Lactobacillus
41
Javali, Mukhatar Ahmed, Mohasin Abdul Khader, Razan Mansour Alqahtani, Muna Jubran Almufarrij, Thamra Mohammed Alqahtani, and Mohamed Khaled Addas. "Spectrophotometric Analysis of Dental Enamel Staining to Antiseptic and Dietary Agents: In Vitro Study." International Journal of Dentistry 2020 (June 5, 2020): 1–5. http://dx.doi.org/10.1155/2020/5429725.
Full textAbstract:
Background/Objectives. Use of antiseptics as an adjunct to a traditional mechanical tooth brushing method has limited their application for long duration because of their side effects such as staining and calculus formation. The objective of this in vitro study is to analyse the staining effects of antiseptic mouthwashes on dental enamel and compare it with those containing nanoparticles, dietary agents, and distilled water (control). Material and Methods. 105 intact premolars extracted for orthodontic reasons and without any caries or anatomical defects were selected for analysis. The samples were randomly divided into 7 different groups of fifteen teeth each for different solutions. A spectrophotometer was used to assess the colorimeter analysis of buccal dental enamel surface at R1 (baseline examination), R2 (24 hours after immersion in different solutions), and R3 (after brushing). Statistical analysis was done using the Kolmogorov–Smirnov test and Levene’s test (p<0.05), respectively. One-way ANOVA was used to compare the difference in color (∆E) between the readings, R1, R2, and R3. Results. The mouthwash containing titanium dioxide (TiO2) nanoparticles produced the greater enamel discoloration compared to that of chlorhexidine. Brushing had little effect on removal of stains induced by all mouthwashes except for dietary solutions (lemon with sodium bicarbonate and olive with laurel) and distilled water (control). Conclusion. The results from this study show that mouthwashes containing TiO2 nanoparticles and other antiseptic mouthwashes cause change in color of the teeth and lead to poor esthetic appearance when compared to dietary and control solutions. Thus, future in vivo studies have to be conducted to confirm these findings as in vitro studies may not provide a reliable simulation of the clinical situations.
42
Zegan, Georgeta, Daniela Anistoroaei, Elena Mihaela Carausu, Eduard Radu Cernei, and loredana Golovcencu. "Amoxicilin and Clavulanic Acid Intercaled Nanostructures for Dentistry Uses." Materiale Plastice 56, no. 2 (June 30, 2019): 396–98. http://dx.doi.org/10.37358/mp.19.2.5193.
Full textAbstract:
Amoxicillin and clavulanic acid are two of the most commonly prescribed antibacterial worldwide for treating oral infectious diseases. Oral health is of big importance for well-being and general health. A few novel drug delivery systems were designed for oral treatment and prophylaxis of different diseases in the oral cavity. This work focused on the latest drug delivery development of the most common oral pathologies, namely, periodontitis, oral mucosal infections, dental caries and oral cancer. Herein we reveal the synthesis, characterization and application of chitosan nanoparticles for intracellular transport of the weakly cell-penetrating amoxicillin and clavulanic acid in order to improve their efficacy on bacterial infections.
43
Domb, Abraham J., Nurit Beyth, and Shady Farah. "QUATERNARY AMMONIUM ANTIMICROBIAL POLYMERS." MRS Proceedings 1569 (2013): 97–107. http://dx.doi.org/10.1557/opl.2013.907.
Full textAbstract:
ABSTRACTPolymers possessing antimicrobial activity have been used for self sterilization surfaces as well as agents for treating contaminated water. Cationic polymers based on quaternary ammonium or guanidine groups have shown high inherent antimicrobial activity where the activity is related to the disruption of the microorganism cell wall. A range of antimicrobial nanoparticles possessing active quaternary ammonium groups with one of the alkyl is a an octyl chain have been synthesized. These nanoparticles were incorporated in dental restoration compositions to form self sterile composites. Quaternary ammonium polyethyleneimine nanoparticles with N-octyl dimethyl residues, demonstrated high antibacterial effect.
44
Herdiyati, Yetty, Harold Eka Atmaja, Mieke Hemiawati Satari, and Dikdik Kurnia. "Potential Antibacterial Flavonoid from Buah Merah (Pandanus conodieus Lam.) Against Pathogenic Oral Bacteria of Enterococcus faecalis ATCC 29212." Open Dentistry Journal 14, no. 1 (September 24, 2020): 433–39. http://dx.doi.org/10.2174/1874210602014010433.
Full textAbstract:
Background: Dental caries is an oral disease generated by pathogenic bacteria, Enterococcus faecalis, which is most frequently found in teeth with pulp necrosis. On the other hand, the use of the medicinal plant to treat pathogenic disease, including caries is an alternative option, which consumes synthetic drug having a side effect. Objective: The purpose of this study is to isolate antibacterial agents from Buah Merah (Pandanus conoideus Lam) and to test the antibacterial activity of those compounds against Enterococcus faecalis ATCC 29212. Methods: Isolation of the antibacterial constituents from Buah Merah used a combinational column chromatography technique which include a normal and reversed-phase. The chosen fraction of each separation is based on the most active fraction. The compounds at various concentrations, 1000 - 20000 μg/mL, were assessed against E. faecalis ATCC 29212 by agar disc diffusion method, and chlorhexidine 2000 μg/mL was used as a positive control. Results: Four compounds isolated from Buah Merah were determined as flavonoid 1, diterpenoid 2, and two fatty acid derivatives 3 and 4. The compounds were then tested against E. faecalis cultured to find inhibition zones, and the study found that only compound 1 identified as Quercetin-3-O-glucose showed an inhibited zone 88 mm at 20000 ppm. Conclusion: This study demonstrated that ethyl acetate fraction of Buah Merah contains an antibacterial flavonoid active against E. faecalis. This research gives information for the use of this plant in herbal medicine and contributes to the necessity of a new antibacterial agent for oral infectious disease. Moreover, this data can be based on information to find the substituted antiseptic applied in the dentistry field.
45
Basri, Dayang Fredalina, Liy Si Tan, Zaleha Shafiei, and Noraziah Mohamad Zin. "In Vitro Antibacterial Activity of Galls ofQuercus infectoriaOlivier against Oral Pathogens." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–6. http://dx.doi.org/10.1155/2012/632796.
Full textAbstract:
The galls ofQuercus infectoriaare commonly used in Malay traditional medicine to treat wound infections after childbirth. In India, they are employed traditionally as dental applications such as that in treatment of toothache and gingivitis. The aim of the present study was to evaluate the antibacterial activity of galls ofQuercus infectoriaOlivier against oral bacteria which are known to cause dental caries and periodontitis. Methanol and acetone extracts were screened against two Gram-positive bacteria (Streptococcus mutansATCC 25175 andStreptococcus salivariusATCC 13419) and two Gram-negative bacteria (Porphyromonas gingivalisATCC 33277 andFusobacterium nucleatumATCC 25586). The screening test of antibacterial activity was performed using agar-well diffusion method. Subsequently, minimum inhibitory concentration (MIC) was determined by using twofold serial microdilution method at a concentration ranging between 0.01 mg/mL and 5 mg/mL. Minimum bactericidal concentration (MBC) was obtained by subculturing microtiter wells which showed no changes in colour of the indicator after incubation. Both extracts showed inhibition zones which did not differ significantly (P<0.05) against each tested bacteria. Among all tested bacteria,S. salivariuswas the most susceptible. The MIC ranges for methanol and acetone extracts were the same, between 0.16 and 0.63 mg/mL. The MBC value, for methanol and acetone extracts, was in the ranges 0.31–1.25 mg/mL and 0.31–2.50 mg/mL, respectively. Both extracts ofQ. infectoriagalls exhibited similar antibacterial activity against oral pathogens. Thus, the galls may be considered as effective phytotherapeutic agents for the prevention of oral pathogens.
46
Pribadi, Nirawati, Yovita Yonas, and Widya Saraswati. "The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract." Dental Journal (Majalah Kedokteran Gigi) 50, no. 2 (June 30, 2017): 97. http://dx.doi.org/10.20473/j.djmkg.v50.i2.p97-101.
Full textAbstract:
Background: Streptococcus mutans (S. mutans) is a bacterium that plays an important role in the pathogenesis of dental caries. Streptococcus mutans produces the glucosyltransferase enzyme which is capable of catalyzing glucan synthesis in the progression of dental caries. Certain treatments involving traditional plant use have been developed to eradicate Streptococcus mutans as a means of preventing the formation of dental caries. One of these is mangosteen pericarp extract containing a number of polyphenols that have the capacity to act as antibacterial agents, namely; tannin, mangostin, and flavonoid. Purpose: The research aimed to investigate the inhibitory power of mangosteen pericarp extract against Streptococcus mutans producing the glucosyltransferase enzyme. Methods: The research used mangosteen pericarp extract at concentrations of 0.39% and 0.78% as the treatments, while 0.12% chlorhexidine gluconate was used as a positive control, and distilled water as a negative control. Each group consisted of six samples. Mangosteen peels extracted with 96% ethanol (maceration method) and mangosteen extract constituted 5% of the total weight of the mangosteen pericarp. Supernatant containing Gtf enzyme produced from a culture medium and centrifuged at 1500 rpm for 10 minutes at 4o C. Glucosyltransferase enzyme activity was measured by analyzing the extensive fructose area by means of High Performance Liquid Chromatography (HPLC). The extensive fructose area was determined according to time retention in each group. Results: Mangosteen peel extract at concentrations of 0.39% and 0.78% demonstrated greater ability than the negative control group (sterile aquades) and similar ability to the positive group (chlorhexidine 0.12%) to inhibit the activity of the Gtf enzyme or S. mutans bacteria. Conclusion: Mangosteen pericarp extract has the ability to inhibit the activity of Streptococcus mutans in producing glucosyltransferase enzyme.
47
Cunha, Luis C. Scalon, Márcio L. Andrade e. Silva, Niege A. J. Cardoso Furtado, Adriana H. C. Vinhólis, Carlos H. Gomes Martins, A. da Silva Filho, and Wilson R. Cunha. "Antibacterial Activity of Triterpene Acids and Semi-Synthetic Derivatives against Oral Pathogens." Zeitschrift für Naturforschung C 62, no. 9-10 (October 1, 2007): 668–72. http://dx.doi.org/10.1515/znc-2007-9-1007.
Full textAbstract:
Triterpene acids (ursolic, oleanoic, gypsogenic, and sumaresinolic acids) isolated from Miconia species, along with a mixture of ursolic and oleanolic acids and a mixture of maslinic and 2-α-hydroxyursolic acids, as well as ursolic acid derivatives were evaluated against the following microorganisms: Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis, which are potentially responsible for the formation of dental caries in humans. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) during the evaluation of the antibacterial activity. All the isolated compounds, mixtures, and semi-synthetic derivatives displayed activity against all the tested bacteria, showing that they are promising antiplaque and anticaries agents. Ursolic and oleanolic acids displayed the most intense antibacterial effect, with MIC values ranging from 30 μg/mL to 80 μg/mL. The MIC values of ursolic acid derivatives, as well as those obtained for the mixture of ursolic and oleanolic acids showed that these compounds do not have higher antibacterial activity when compared with the activity observed with either ursolic acid or oleanolic acid alone. With regard to the structure-activity relationship of triterpene acids and derivatives, it is suggested that both hydroxy and carboxy groups present in the triterpenes are important for their antibacterial activity against oral pathogens.
48
Owusu-Boadi, Ernest, Mainprice Akuoko Essuman, Gabriel Mensah, Emmanuel Ayamba Ayimbissa, and Alex Boye. "Antimicrobial Activity against Oral Pathogens Confirms the Use of Musa paradisiaca Fruit Stalk in Ethnodentistry." Evidence-Based Complementary and Alternative Medicine 2021 (September 4, 2021): 1–9. http://dx.doi.org/10.1155/2021/8663210.
Full textAbstract:
Background. Ethnodentistry is the use of indigenous oral cleansing agents such as plant parts by local folks not only to maintain oral hygiene but also to treat oral infections. Mostly, ethnodentistry is inspired by traditions and belief systems of local communities. Musa paradisiaca is extensively cultivated and used in many cultures for its nutritional and medicinal values. In Ghana, the fruit stalk of Musa paradisiaca is used as an oral cleansing agent to maintain oral hygiene; yet this folk claim remains to be ascertained scientifically. Objective. The study assessed the antibacterial and antifungal effects of three extract fractions (aqueous, ethanol, and ethyl acetate fractions) of Musa paradisiaca fruit stalk against Lactobacillus acidophilus, Aggregatibacter actinomycetemcomitans, and Candida albicans, common oral pathogens implicated in dental caries and periodontitis. Materials and Methods. Aqueous, ethanol, and ethyl acetate fractions of Musa paradisiaca fruit stalk were prepared by cold maceration and qualitatively screened for their phytochemical composition. Antimicrobial effects of the three extract fractions were assessed by using serial broth dilutions at increasing concentrations (62.5, 125, 250, 500, and 1000 µg/ml) and compared to standard antimicrobial agents (erythromycin, doxycycline, and fluconazole). Subsequently, the absorbances of the microbial suspensions treated with increasing concentrations of the extract fractions were measured at 450 nm, and the cell densities were determined. Results. Except for the aqueous extract, which was less effective in decreasing microbial growth, the ethyl acetate and the ethanol extract fractions demonstrated antimicrobial efficacies comparable to those of the standard drugs. All three extract fractions demonstrated concentration-dependent growth inhibitory effects on the tested oral pathogens although not as effective as the standard drugs used. Conclusion. Musa paradisiaca fruit stalk has demonstrated antimicrobial effects against Lactobacillus acidophilus, Aggregatibacter actinomycetemcomitans, and Candida albicans, common oral pathogens implicated in dental caries and periodontitis, and this finding confirms in part folk use of Musa paradisiaca fruit stalk as a traditional dental care agent. Thus, the fruit stalk of Musa paradisiaca could be explored for use as a cheap and readily available dental care agent for people entrapped in the poverty line.
49
Mitwalli, Heba, Abdulrahman A. Balhaddad, Rashed AlSahafi, Thomas W. Oates, Mary Anne S. Melo, Hockin H. K. Xu, and Michael D. Weir. "Novel CaF2 Nanocomposites with Antibacterial Function and Fluoride and Calcium Ion Release to Inhibit Oral Biofilm and Protect Teeth." Journal of Functional Biomaterials 11, no. 3 (August 1, 2020): 56. http://dx.doi.org/10.3390/jfb11030056.
Full textAbstract:
(1) Background: The objective of this study was to develop a novel dental nanocomposite containing dimethylaminohexadecyl methacrylate (DMAHDM), 2-methacryloyloxyethyl phosphorylcholine (MPC), and nanoparticles of calcium fluoride (nCaF2) for preventing recurrent caries via antibacterial, protein repellent and fluoride releasing capabilities. (2) Methods: Composites were made by adding 3% MPC, 3% DMAHDM and 15% nCaF2 into bisphenol A glycidyl dimethacrylate (Bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) (denoted BT). Calcium and fluoride ion releases were evaluated. Biofilms of human saliva were assessed. (3) Results: nCaF2+DMAHDM+MPC composite had the lowest biofilm colony forming units (CFU) and the greatest ion release; however, its mechanical properties were lower than commercial control composite (p < 0.05). nCaF2+DMAHDM composite had similarly potent biofilm reduction, with mechanical properties matching commercial control composite (p > 0.05). Fluoride and calcium ion releases from nCaF2+DMAHDM were much more than commercial composite. Biofilm CFU on composite was reduced by 4 logs (n = 9, p < 0.05). Biofilm metabolic activity and lactic acid were also substantially reduced by nCaF2+DMAHDM, compared to commercial control composite (p < 0.05). (4) Conclusions: The novel nanocomposite nCaF2+DMAHDM achieved strong antibacterial and ion release capabilities, without compromising the mechanical properties. This bioactive nanocomposite is promising to reduce biofilm acid production, inhibit recurrent caries, and increase restoration longevity.
50
Choi, Soo-Hyoen, Yong-Seok Jang, Jong-Hwa Jang, Tae-Sung Bae, Sook-Jeong Lee, and Min-Ho Lee. "Enhanced antibacterial activity of titanium by surface modification with polydopamine and silver for dental implant application." Journal of Applied Biomaterials & Functional Materials 17, no. 3 (July 2019): 228080001984706. http://dx.doi.org/10.1177/2280800019847067.
Full textAbstract:
Background: Biofilm formation and microbial colonization on the surface of implant devices may cause dental caries and peri-implantitis. Therefore, various surface treatments have been developed to improve the antibacterial activity of titanium implant. Methods: Silver-loaded polydopamine coating was formed by immersing pure titanium in dopamine hydrochloride/HCl buffer solution for 24 h in 50 mL silver nitrate solutions with different concentrations for 30 min. Microbial growth inhibition and microbial growth curve analyses for bacterial solutions of Streptococcus mutans and Porphyromonas gingivalis incubated with the specimens were respectively conducted by counting the numbers of colonies on agar solid medium and by measuring absorbance using enzyme-linked immunosorbent assay reader. Results: Silver nanoparticles were uniformly distributed over the whole surface of the polydopamine and silver-coated titanium specimens. The numbers of microbial colonies for both bacteria cultured with surface-modified titanium were significantly lower than those cultured with uncoated titanium. When Streptococcus mutans and Porphyromonas gingivalis were cultured with surface-modified titanium, the lag phase of the growth curves for both bacteria was continually maintained, whereas the lag phase for Streptococcus mutans and Porphyromonas gingivalis changed to exponential phase after 9 and 15 h, respectively, when both bacteria were cultured with uncoated titanium. Conclusion: It was confirmed that the coating of polydopamine and silver on the surface of titanium effectively retards the microbial growth, which can cause the formation of biofilm and pathogenesis of gum disease in the mouth.