Journal articles: 'Dental Pulp Diseases'

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Hu, Lei, Zhenhua Gao, Junji Xu, Zhao Zhu, Zhipeng Fan, Chunmei Zhang, Jinsong Wang, and Songlin Wang. "Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration." BioMed Research International 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/9342714.

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Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases.

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Khan, Mohammad Imran, Abhisheik Khare, Khushboo Arif, Sameera Shamim Khan, Abdullah Nasir, and Shafik Lari. "Dental pulp stones and their correlation with metabolic diseases." Journal of Oral and Maxillofacial Pathology 28, no. 2 (April 2024): 192–99. http://dx.doi.org/10.4103/jomfp.jomfp_536_23.

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Abstract Background: Dental pulp calcifications or pulp stones are calcified structures found in dental pulp, mostly around or enclosing a blood vessel. The formation of these calcifications begins with concentric layers of calcified tissue within which remnants of necrotic and calcified cells may be present. The calcifications of thrombi in blood vessels, called phleboliths, may also serve as nidi for denticles. In metabolic diseases such as diabetes, hypertension or poor periodontal health, there are obvious changes in blood vessels and vascularization. In our study, we observed histopathological sections of dental pulp and correlated systemic diseases such as diabetes and hypertension with poor periodontal health and dental pulp stones. Aim: The aim of our study was to evaluate the histopathology of dental pulp stones, their distribution among various age groups and sexes and to identify any correlations between pulp stone formation and systemic diseases such as type II diabetes and hypertension. Materials and Methods: Samples from 100 patients with metabolic diseases such as type II diabetes and hypertension were collected. The pulp was extirpated from the teeth that were undergoing root canal treatment, and the teeth were extracted. The collected pulp sample was fixed in 10% formaline neutral buffer, subjected to routine histopathological procedures and stained with haematoxylin and eosin. The pulp of teeth extracted for orthodontic treatment was considered a control for patients with no metabolic disease. Results: There was a definite relationship between increased pulp stones and metabolic diseases such as type II diabetes and hypertension; likewise, poor periodontal health was significantly related to pulp stones.

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Bains, Sandeep Kumar, Archana Bhatia, Harkanwal Preet Singh, Swati Swagatika Biswal, Shashi Kanth, and Srinivas Nalla. "Prevalence of Coronal Pulp Stones and Its Relation with Systemic Disorders in Northern Indian Central Punjabi Population." ISRN Dentistry 2014 (April 22, 2014): 1–5. http://dx.doi.org/10.1155/2014/617590.

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Aim. To estimate the prevalence of coronal pulp stones in the molar teeth of dental outpatients of Sunam, Sangrur district, Punjab, India, to report any association between occurrence of pulp stones with age, gender, dental arch, side, and dental status and to find out correlation between pulp stones with dental and systemic diseases. Materials and Methods. 500 routine dental outpatients within age group of 18–67 years were involved in the study. Molar bitewing of left and right side of each patient was taken with XCP bitewing instrument and size 2 film. The presence or absence of pulp stones was recorded. Chi-square analysis was used to record the prevalence of pulp stones and to compare it with demographic and systemic factors. Results. Overall prevalence of pulp stones was 41.8%. Pulp stones were significantly higher in maxilla (11.59%) than mandible (6.54%), left side than right side, and first molar than other molars. Higher numbers of pulp stones were recorded in patients with cardiovascular disease (38.89%) than with cholelithiasis and renal lithiasis. Conclusion. Pulp stones were higher in maxillary arch than mandibular arch and in females than males. Cardiovascular patients had higher number of pulp stones than other groups.

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Nijakowski, Kacper, Martyna Ortarzewska, Jakub Jankowski, Anna Lehmann, and Anna Surdacka. "The Role of Cellular Metabolism in Maintaining the Function of the Dentine-Pulp Complex: A Narrative Review." Metabolites 13, no. 4 (April 5, 2023): 520. http://dx.doi.org/10.3390/metabo13040520.

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The cellular metabolic processes ensure the physiological integrity of the dentine-pulp complex. Odontoblasts and odontoblast-like cells are responsible for the defence mechanisms in the form of tertiary dentine formation. In turn, the main defence reaction of the pulp is the development of inflammation, during which the metabolic and signalling pathways of the cells are significantly altered. The selected dental procedures, such as orthodontic treatment, resin infiltration, resin restorations or dental bleaching, can impact the cellular metabolism in the dental pulp. Among systemic metabolic diseases, diabetes mellitus causes the most consequences for the cellular metabolism of the dentine-pulp complex. Similarly, ageing processes present a proven effect on the metabolic functioning of the odontoblasts and the pulp cells. In the literature, several potential metabolic mediators demonstrating anti-inflammatory properties on inflamed dental pulp are mentioned. Moreover, the pulp stem cells exhibit the regenerative potential essential for maintaining the function of the dentine-pulp complex.

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Dhanushkodi, Anandh, Christopher Shamir, and Chaitra Venugopal. "Dental pulp stem cells for treating neurodegenerative diseases." Neural Regeneration Research 10, no. 12 (2015): 1910. http://dx.doi.org/10.4103/1673-5374.169629.

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Hu, Nan, Weiping Li, Wentao Jiang, Jin Wen, and Shensheng Gu. "Creating a Microenvironment to Give Wings to Dental Pulp Regeneration—Bioactive Scaffolds." Pharmaceutics 15, no. 1 (January 3, 2023): 158. http://dx.doi.org/10.3390/pharmaceutics15010158.

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Dental pulp and periapical diseases make patients suffer from acute pain and economic loss. Although root canal therapies, as demonstrated through evidence-based medicine, can relieve symptoms and are commonly employed by dentists, it is still difficult to fully restore a dental pulp’s nutrition, sensory, and immune-regulation functions. In recent years, researchers have made significant progress in tissue engineering to regenerate dental pulp in a desired microenvironment. With breakthroughs in regenerative medicine and material science, bioactive scaffolds play a pivotal role in creating a suitable microenvironment for cell survival, proliferation, and differentiation, following dental restoration and regeneration. This article focuses on current challenges and novel perspectives about bioactive scaffolds in creating a microenvironment to promote dental pulp regeneration. We hope our readers will gain a deeper understanding and new inspiration of dental pulp regeneration through our summary.

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Ali, Azam, Maree Gould, and Karl Lyons. "Development of an Organic–Inorganic Nanostructured Hybrid Dental Biocomposite." Journal of Nanoscience and Nanotechnology 20, no. 8 (August 1, 2020): 5252–59. http://dx.doi.org/10.1166/jnn.2020.18527.

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Dental pathologies such as caries is one of the most prevalent diseases worldwide. Dental pulp contains stem cells capable of regenerating the dentine in the tooth, consequently, healthy dental pulp is essential for long term tooth survival. The aim of this study was to incorporate a variety of polymers that provide strength, an antibacterial substance and a protein-based polymer to provide cell support. These components were combined into a triphasic hybrid dental biocomposite (3HB), that together could provide regenerative properties for the pulp tissue. The 3HB biocomposite was incorporated into Organic–inorganic nanostructured materials such as Mineral Trioxide Aggregate (MTA) as a base to assemble a hybrid dental biocomposite. The effects of the 3HB on cytotoxicity was examined in mouse dental pulp cells, MDPC-23. In vitro studies showed that 3HB supported the proliferative growth of the cells significantly more than the no treatment control. 3HB also caused little stress to the cells and supported cell viability. Fourier transform infrared (FTIR) spectra confirmed the presence of polymer functional groups within the 3HB biocomposite. Therefore, 3HB compound has the potential to be applied as a pulp wound dressing providing superior cytocompatibility than the present options but also may be indispensable for the regeneration of dental pulp.

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Grawish, Mohammed E. "Human dental pulp stem/stromal cells in clinical practice." World Journal of Stem Cells 16, no. 2 (February 26, 2024): 54–57. http://dx.doi.org/10.4252/wjsc.v16.i2.54.

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Dental pulp stem/stromal cells (DPSCs) are fibroblast-like, neural crest-derived, and multipotent cells that can differentiate into several lineages. They are relatively easy to isolate from healthy and inflamed pulps, with little ethical concerns and can be successfully cryopreserved and thawed. The therapeutic effects of DPSCs derived from animal or human sources have been extensively studied through in-vitro and in-vivo animal experiments and the findings indicated that DPSCs are effective not only for dental diseases but also for systemic diseases. Understanding that translational research is a critical step through which the fundamental scientific discoveries could be translated into applicable diagnostics and therapeutics that directly benefit humans, several clinical studies were carried out to generate evidence for the efficacy and safety of autogenous or allogeneic human DPSCs (hDPSCs) as a treatment modality for use in cell-based therapy, regenerative medicine/dentistry and tissue engineering. In clinical medicine, hDPSCs were effective for treating acute ischemic stroke and human exfoliated deciduous teeth-conditioned medium (SHED-CM) repaired vascular damage of the corpus cavernous, which is the main cause of erectile dysfunction. Whereas in clinical dentistry, autologous SHED was able to regenerate necrotic dental pulp after implantation into injured teeth, and micrografts enriched with autologous hDPSCs and collagen sponge were considered a treatment option for human intrabony defects. In contrast, hDPSCs did not add a significant regenerative effect when they were used for the treatment of post-extraction sockets. Large-scale clinical studies across diverse populations are still lacking to provide robust evidence on the safety and efficacy of hDPSCs as a new treatment option for various human diseases including dental-related problems.

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Priyan GL, Saravana, Subachanya Ramalingam, and Yogeshwari Udhayakumar. "Human dental pulp stem cells and its applications in regenerative medicine – A literature review." Journal of Global Oral Health 2 (September 25, 2019): 59–67. http://dx.doi.org/10.25259/jgoh_54_2019.

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Human dental pulp-derived stem cells have varied applications in regenerative medicine. Dental pulp stem cells (DPSCs) are considered to be neural crest cells. They are known to have higher regenerative potential than the bone marrow-derived mesenchymal stem cells. DPSCs have multipotency, immunomodulatory function, and self-renewal capacity. They are highly proliferative, clonogenic and are capable of differentiating into adipocytes, neural cells, odontoblasts, and various other cells. DPSCs are effective for various diseases, such as spinal cord injuries, Parkinson’s disease, Alzheimer’s disease, cerebral ischemia, myocardial infarction, muscular dystrophy, diabetes, liver diseases, eye diseases, immune diseases, and oral diseases. This article provides an overview of properties and regenerative applications of human DPSCs.

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Xie, Zhuo, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, and Zhengmei Lin. "Functional Dental Pulp Regeneration: Basic Research and Clinical Translation." International Journal of Molecular Sciences 22, no. 16 (August 20, 2021): 8991. http://dx.doi.org/10.3390/ijms22168991.

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Pulpal and periapical diseases account for a large proportion of dental visits, the current treatments for which are root canal therapy (RCT) and pulp revascularisation. Despite the clinical signs of full recovery and histological reconstruction, true regeneration of pulp tissues is still far from being achieved. The goal of regenerative endodontics is to promote normal pulp function recovery in inflamed or necrotic teeth that would result in true regeneration of the pulpodentinal complex. Recently, rapid progress has been made related to tissue engineering-mediated pulp regeneration, which combines stem cells, biomaterials, and growth factors. Since the successful isolation and characterisation of dental pulp stem cells (DPSCs) and other applicable dental mesenchymal stem cells, basic research and preclinical exploration of stem cell-mediated functional pulp regeneration via cell transplantation and cell homing have received considerably more attention. Some of this effort has translated into clinical therapeutic applications, bringing a ground-breaking revolution and a new perspective to the endodontic field. In this article, we retrospectively examined the current treatment status and clinical goals of pulpal and periapical diseases and scrutinized biological studies of functional pulp regeneration with a focus on DPSCs, biomaterials, and growth factors. Then, we reviewed preclinical experiments based on various animal models and research strategies. Finally, we summarised the current challenges encountered in preclinical or clinical regenerative applications and suggested promising solutions to address these challenges to guide tissue engineering-mediated clinical translation in the future.

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Liu, Fei, Jing Xiao, Lei-Hui Chen, Yu-Yue Pan, Jun-Zhang Tian, Zhi-Ren Zhang, and Xiao-Chun Bai. "Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization." World Journal of Stem Cells 16, no. 3 (March 26, 2024): 287–304. http://dx.doi.org/10.4252/wjsc.v16.i3.287.

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BACKGROUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative medicine. Stem cells can self-organise into microsized organ units, partially modelling tissue function and regeneration. Dental pulp organoids have been used to recapitulate the processes of tooth development and related diseases. However, the lack of vasculature limits the utility of dental pulp organoids. AIM To improve survival and aid in recovery after stem cell transplantation, we demonstrated the three-dimensional (3D) self-assembly of adult stem cell-human dental pulp stem cells (hDPSCs) and endothelial cells (ECs) into a novel type of spheroid-shaped dental pulp organoid in vitro under hypoxia and conditioned medium (CM). METHODS During culture, primary hDPSCs were induced to differentiate into ECs by exposing them to a hypoxic environment and CM. The hypoxic pretreated hDPSCs were then mixed with ECs at specific ratios and conditioned in a 3D environment to produce prevascularized dental pulp organoids. The biological characteristics of the organoids were analysed, and the regulatory pathways associated with angiogenesis were studied. RESULTS The combination of these two agents resulted in prevascularized human dental pulp organoids (Vorganoids) that more closely resembled dental pulp tissue in terms of morphology and function. Single-cell RNA sequencing of dental pulp tissue and RNA sequencing of Vorganoids were integrated to analyse key regulatory pathways associated with angiogenesis. The biomarkers forkhead box protein O1 and fibroblast growth factor 2 were identified to be involved in the regulation of Vorganoids. CONCLUSION In this innovative study, we effectively established an in vitro model of Vorganoids and used it to elucidate new mechanisms of angiogenesis during regeneration, facilitating the development of clinical treatment strategies.

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Zhuang, Xueying, Lingli Ji, Huan Jiang, Yao Liu, Xuemei Liu, Jing Bi, Weidong Zhao, Zhenjiang Ding, and Xu Chen. "Exosomes Derived from Stem Cells from the Apical Papilla Promote Dentine-Pulp Complex Regeneration by Inducing Specific Dentinogenesis." Stem Cells International 2020 (May 27, 2020): 1–10. http://dx.doi.org/10.1155/2020/5816723.

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Regenerative endodontic procedures (REPs) are a new option for the treatment of dental pulp or periapical diseases in permanent teeth with open apices. Histologically, the new tissues formed in the root canal after REPs are mainly cementum- or bone-like mineralised tissues, but not the real dentine-pulp complex. Therefore, how to promote dentine-pulp complex regeneration and improve the clinical effects of REPs has become a prominent research topic. Stem cells from apical papilla (SCAP) are derived from the dental papilla that can differentiate into primary odontoblasts and dental pulp cells that produce root dentine and dental pulp. Exosomes are the key regulator for the paracrine activity of stem cells and can influence the function of recipient cells. In this study, SCAP-derived exosomes (SCAP-Exo) were introduced into the root fragment containing bone marrow mesenchymal stem cells (BMMSCs) and transplanted subcutaneously into immunodeficient mice. We observed that dental pulp-like tissues were present and the newly formed dentine was deposited onto the existing dentine in the root canal. Afterwards, the effects of SCAP-Exo on the dentinogenesis of BMMSCs were elucidated in vitro. We found that the gene and protein expression of dentine sialophosphoprotein and mineralised nodule formation in BMMSCs treated with SCAP-Exo were significantly increased. In summary, SCAP-Exo were endocytosed by BMMSCs and obviously improved their specific dentinogenesis. The use of exosomes derived from dental stem cells could comprise a potential therapeutic approach for dentine-pulp complex regeneration in REPs.

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Gan, Lu, Ying Liu, Dixin Cui, Yue Pan, Liwei Zheng, and Mian Wan. "Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application." Stem Cells International 2020 (September 1, 2020): 1–17. http://dx.doi.org/10.1155/2020/8864572.

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Human mesenchymal stem cells (hMSCs) are multipotent cells, which exhibit plastic adherence, express specific cell surface marker spectrum, and have multi-lineage differentiation potential. These cells can be obtained from multiple tissues. Dental tissue-derived hMSCs (dental MSCs) possess the ability to give rise to mesodermal lineage (osteocytes, adipocytes, and chondrocytes), ectodermal lineage (neurocytes), and endodermal lineages (hepatocytes). Dental MSCs were first isolated from dental pulp of the extracted third molar and till now they have been purified from various dental tissues, including pulp tissue of permanent teeth and exfoliated deciduous teeth, apical papilla, periodontal ligament, gingiva, dental follicle, tooth germ, and alveolar bone. Dental MSCs are not only easily accessible but are also expandable in vitro with relative genomic stability for a long period of time. Moreover, dental MSCs have exhibited immunomodulatory properties by secreting cytokines. Easy accessibility, multi-lineage differentiation potential, and immunomodulatory effects make dental MSCs distinct from the other hMSCs and an effective tool in stem cell-based therapy. Several preclinical studies and clinical trials have been performed using dental MSCs in the treatment of multiple ailments, ranging from dental diseases to nondental diseases. The present review has summarized dental MSC sources, multi-lineage differentiation capacities, immunomodulatory features, its potential in the treatment of diseases, and its application in both preclinical studies and clinical trials. The regenerative therapeutic strategies in dental medicine have also been discussed.

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Pratiwi, Ariyati Retno, and Dinia Fitri Salsabila. "Peran Decellurarized Dental Pulp Sebagai Kandidat Biomaterial Baru Dalam Regenerasi Pulpa : Tinjauan Literatur." STOMATOGNATIC - Jurnal Kedokteran Gigi 21, no. 1 (March 31, 2024): 8. http://dx.doi.org/10.19184/stoma.v21i1.47327.

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Pulp necrosis is one of the main diseases that are most often found in the teeth and mouth. Root canal treatment is the main solution for pulp necrosis, but its implementation can lead to tooth death and recurrent infections. Regenerating the pulp by transplanting scaffolds, stem cells, and growth factors into the root canal is an alternative that can be offered to treat pulpal disease by keeping the teeth vital. Scaffold plays an important role in pulp regeneration, one of which is the ECM scaffold derived from pulp which combines the micro-collagen structure along with bioactive molecules that are important for pulp regeneration and able to promote the differentiation and proliferation of stem cells. This literature review aimed to identify the role of decellularized dental pulp as a new biomaterial candidate in pulp regenerations. The method being used in this review is systematic literature review using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) method. Decellularized Dental Pulp as a New Biomaterial Candidate has a role in pulp regeneration.

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Muñoz-Carrillo, José Luis, Silverio Jafet Vázquez-Alcaraz, Jazmín Monserrat Vargas-Barbosa, Luis Guillermo Ramos-Gracia, Israel Alvarez-Barreto, Alejandro Medina-Quiroz, and Karla Karina Díaz-Huerta. "The Role of microRNAs in Pulp Inflammation." Cells 10, no. 8 (August 20, 2021): 2142. http://dx.doi.org/10.3390/cells10082142.

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The dental pulp can be affected by thermal, physical, chemical, and bacterial phenomena that stimulate the inflammatory response. The pulp tissue produces an immunological, cellular, and vascular reaction in an attempt to defend itself and resolve the affected tissue. The expression of different microRNAs during pulp inflammation has been previously documented. MicroRNAs (miRNAs) are endogenous small molecules involved in the transcription of genes that regulate the immune system and the inflammatory response. They are present in cellular and physiological functions, as well as in the pathogenesis of human diseases, becoming potential biomarkers for diagnosis, prognosis, monitoring, and safety. Previous studies have evidenced the different roles played by miRNAs in proinflammatory, anti-inflammatory, and immunological phenomena in the dental pulp, highlighting specific key functions of pulp pathology. This systematized review aims to provide an understanding of the role of the different microRNAs detected in the pulp and their effects on the expression of the different target genes that are involved during pulp inflammation.

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Fabrizio Nifosì, Antonio, Pablo Castelló, Lorenzo Nifosí, and Gianfilippo Nifosí. "Dental alterations in sickle cell anemia." Edorium Journal of Dentistry 4, no. 1 (June 1, 2017): 1–5. http://dx.doi.org/10.5348/d01-2017-23-ra-3.

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The epidemiology of the dental disease in sickle cell anemia is not adequately described. The aim of this work is either the evaluation of literature on the topic, or to describe if the disease itself predisposes to a dental pathology, and eventually also if the latter can influence its course. We selected many cases and reviews in order to identify the dental diseases related to sickle cell anemia. Caries and periodontitis are not directly caused by disease. However, an inflammatory state caused by a dental infection can trigger or precipitate a sickle crisis. Malocclusion angle class II is particularly frequent, as a result of bone facial changes and/or related to muscle imbalance. Temporomandibular joint disorder is possible. Asymptomatic pulp necrosis is due to the sickling that causes vasocclusion within microcirculation of the dental pulp. Large population-based studies are needed in order to clarify the dental involvement in this disease. A strict collaboration between the dentist and the hematologist is essential.

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Kopetskiy, Igor S., Natalja V. Polunina, Ludmila V. Pobozhieva, Yuliya V. Sheveluk, and Maria K. Makeeva. "The prevalence of dental diseases and medical and social characteristics in young people." Russian Medicine 30, no. 1 (March 14, 2024): 27–36. http://dx.doi.org/10.17816/medjrf625758.

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BACKGROUND: Despite ongoing treatment and prevention programmes, the prevalence of dental diseases remains high, particularly in young people. The prevalence of oral diseases is influenced by several socioeconomic factors, including age, place of residence, fluoride intake, individual oral care, diet and general somatic disease. The study of these factors is an urgent issue in dentistry. AIM: To study the medical and social characteristics of young people and their effect on the prevalence of dental diseases. METHODS: The oral cavities in 917 patients aged 18–44 years who had no history of dental treatment for at least 6 months were examined. The indices of DMF, Silness–LÖe, Green–Vermillion, PMA, CPITN, detection of noncarious lesions of teeth, malocclusion and diseases of the pulp, periapical tissues and periodontal were determined. To establish the influence of social and hygienic factors on the condition of the oral cavity, the survey responses of the study participants were analysed. RESULTS: In young people, the rates of dental caries, diseases of the pulp and periapical tissues and inflammatory periodontal diseases were 94.2%, 47.4% and 86.8%, respectively. Among the medical and social factors determining the high prevalence of dental diseases, unsatisfactory hygienic condition of the oral cavity, visits to the dentist less than once a year and professional oral hygiene less than once a year should be noted. CONCLUSION: The high prevalence of dental diseases in young people requires a differentiated approach to therapeutic and preventive measures, using medical and social factors.

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Susanty, Elva, and Yusdiana Yusdiana. "Peningkatan Pengetahuan dan Perilaku Personal Hygiene Anak di Panti Asuhan Baiturrahmah Kampar." Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat 4, no. 1 (June 21, 2022): 211–16. http://dx.doi.org/10.24036/abdi.v4i1.223.

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Personal hygiene is an effort to maintain personal hygiene and health. Knowledge about personal hygiene needs to be known by children from an early age so that children avoid diseases such as worms, dental caries, and others. Increased knowledge will improve personal hygiene behavior in a better behavior. This community service activity aimed to increase children's knowledge about personal hygiene, improve personal hygiene behavior, and to find out the description of dental and oral health in children at the Baiturrahmah Orphanage, Kampar Regency. The method used in this community service activity was education, dental and oral health check up. This community service activities began with pretest, education, post test, and dental and oral health check up. The results of this service activity showed an increase in knowledge about personal hygiene, especially about dental and oral health, as well as hand washing WHO 6 steps by 18.01%, there were 59.26% of children suffering from dental and oral diseases such as superficial caries, chronic pulpitis, pulp gangrene, pulp polyps, crowding, and impacted teeth. It is necessary to carry out routine dental and oral health check up on children at the Baiturrahmah Orphanage and familiarize children with good personal hygiene behavior.

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Alisa, Wichaidit, Patinotham Namthip, Nukaeow Kullanun, and Kaewpitak Aunwaya. "Expression of PAR2 and NF-κB in human primary dental pulp odontoblasts during the progression of caries." Open Journal of Cell and Protein Science 5, no. 1 (May 6, 2022): 001–4. http://dx.doi.org/10.17352/ojcps.000004.

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Increased proteinase-activated receptor-2 (PAR2) expression is observed in various diseases related to inflammation. However, the expression of PAR2 in odontoblasts in response to dental caries has not been investigated. Therefore, to explore the functions of odontoblasts during the progression of carious infection, we measured PAR2 and NF-κB expression using immunofluorescence techniques in the odontoblast layer and pulpocytes in the sub-odontoblast region of 44 teeth extracted from children undergoing dental treatment (eight sound samples, 13 early carious samples, 16 advanced carious samples, and eight exposed pulp samples). PAR2 and NF-κB were expressed at moderate levels in sound teeth with non-carious pulp, and the expression levels changed as caries progressed. PAR2 was significantly upregulated in the odontoblast layer during early-stage and advanced-stage caries, and reduced below healthy levels in teeth with exposed pulp. NF-κB was significantly upregulated in early-stage caries and significantly downregulated in advanced-stage and late-stage caries. Moreover, in the sub-odontoblast region, NF-κB expression increased with the progression of caries. Overall, this study suggests PAR2 may represent a crucial cell signalling receptor in the dentine-pulp complex during dental inflammation, and that NF-κB may be one of the key pathways that regulate inflammatory immune responses in the dental pulp.

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Huang, Qin, Zucen Li, Ping Lyu, Xuedong Zhou, and Yi Fan. "Current Applications and Future Directions of Lasers in Endodontics: A Narrative Review." Bioengineering 10, no. 3 (February 26, 2023): 296. http://dx.doi.org/10.3390/bioengineering10030296.

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The utilization of lasers has been regarded as a novel technique for the purposes of clinical use in the dental field. Recently, numerous studies have been conducted on the potential applications of laser therapy in endodontics. Moreover, due to their ablation, penetrability, and disinfection capabilities, lasers have performed well with respect to endodontic treatments, including root canal treatment, vital pulp therapy (pulp capping and pulpotomy), dentinal hypersensitivity treatment, and management of dental pain related to pulp and periradicular disease. In particular, the superiorities of laser-aided pulp therapy are emphasized through condensed clinical controlled trials, and histological studies, in this review. Moreover, the ingenious use of laser applications with respect to aiding in the acceleration of root development and the extraction of foreign matters (i.e., broken files and fiber posts) in canals has quickly become the cutting-edge trend of current research. This review offers a summary and discussion of the current literature on all the aforementioned laser applications. Moreover, the characteristics of laser devices, including erbium lasers, neodymium-doped lasers, CO2 lasers, and diode lasers, are detailed and discussed here, providing useful references for laser application in endodontics. We also focus on the different wavelengths with respect to the lasers that are applied in endodontics. High-power lasers perform well as operative instruments; in addition, low-level lasers lead to the regulation of pulp inflammation, and the promotion of pulp healing. This narrative review provides a summary of the advanced applications of lasers in conjunction with various devices in the practice of endodontics, and aims to inspire innovative perspectives on lasers in the context of the treatment of dental diseases, especially pulp diseases, in the future.

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Kim, Joo-Hee, Hoi-In Jung, Ik-Hwan Kim, Jaeho Lee, and Chung-Min Kang. "Impact of Coronavirus Disease 2019 on Dental Service Utilization of Korean Children and Adolescents." JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY 49, no. 2 (May 31, 2022): 206–16. http://dx.doi.org/10.5933/jkapd.2022.49.2.206.

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This study aimed to investigate the frequency of dental visits after coronavirus disease 2019 (COVID-19) in children and adolescents in comparison to that of adults. The data on the number of dental visits on the basis of different classification and treatment codes for all the age groups were provided by the Health Insurance Review and Assessment Service from January 2017 to December 2020. To reduce the impact of the population change, dental visits per 1000 people was used. After the start of the pandemic, dental visits per 1000 people decreased in all age groups when analyzed for the diseases of pulp and periapical tissues classification code and dental visits per 1000 people decreased over 5 years age groups when analyzed for the pulp treatment code. Regarding the restorative treatment code, visits in the 0 - 19 years age group decreased after the pandemic. Based on the comparison of the number of confirmed COVID-19 cases to that of monthly dental visits by treatment codes, the change rate of pulp and restorative treatments gradually decreased after the waves of the pandemic in February and August 2020 in Korea. Furthermore, dental visits per 1000 people for gingivitis and periodontal disease classification code increased after the pandemic in all age groups. The rate of decrease in dental visits per 1000 people for restorative treatments in children and adolescents was higher than that in adults. The rate of decrease in the number of dental visits for multi-visit pulp treatments showed a rapid decrease as compared to that of 1-visit pulp treatments after the pandemic. This study identified changes in the number of dental visits of children and adolescents after COVID-19 transmission in comparison to that observed in adults.

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Ohkoshi, Shogo, Hajime Hara, Haruka Hirono, Kazuhiko Watanabe, and Katsuhiko Hasegawa. "Regenerative medicine using dental pulp stem cells for liver diseases." World Journal of Gastrointestinal Pharmacology and Therapeutics 8, no. 1 (2017): 1. http://dx.doi.org/10.4292/wjgpt.v8.i1.1.

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Giorgini, E., S. Sabbatini, C. Conti, C. Rubini, R. Rocchetti, M. Fioroni, L. Memè, and G. Orilisi. "Fourier Transform Infrared Imaging analysis of dental pulp inflammatory diseases." Oral Diseases 23, no. 4 (February 22, 2017): 484–91. http://dx.doi.org/10.1111/odi.12635.

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Bratus, A. E., and S. V. Kuznecov. "THE PRINCIPLES OF USING SPECIFIC TREATMENT PROTOCOLS IN THE DAILY PRACTICE OF AN ENDODONTIST." National Association of Scientists 1, no. 28(55) (June 15, 2020): 31–32. http://dx.doi.org/10.31618/nas.2413-5291.2020.1.55.211.

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The main goals in dental practice are the high quality of the work, reductionof the risk of medical errors and effective diagnosis and treatment of oral tissues diseases; the whole multilevel system of medical care is willing to increase these indicators. Therefore, it is important to find ways to improve and maintain the dental care for the population [1]. One of these ways is the implementation of dental care standards. These standards are developed by the Dental Association of Russia and updated in 2018. There are specific algorithms and clinical recommendations for the treatment of patients with pulp and periapical tissue diseases

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Vigil, Antony, and Subbiah Bharathi. "Diagnosis of Pulpitis from Dental Panoramic Radiograph Using Histogram of Gradients with Discrete Wavelet Transform and Multilevel Neural Network Techniques." Traitement du Signal 38, no. 5 (October 31, 2021): 1549–55. http://dx.doi.org/10.18280/ts.380532.

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Radiograph plays the major role of diagnosis, treatment and surgery in the Dental field. There are many types of Intra and extra oral radiographs in which Dental Panoramic Radiograph helps in visualising the full view of the oral cavity. Pulpitis is the dental diseases caused due to the inflammation of the dental pulp from untreated caries, trauma or multiple restorations which leads to Apical Periodontitis. To predict the severity of pulp vitality pulp inflammation has to be evaluated. Radiographs helps the dentist in diagnosing the extent of tooth decay and inflammation. An automatic diagnostic model is proposed using robust algorithms to diagnose pulpits. Dental Panoramic Radiograph is used in the proposed research to diagnose the pulpitis and to classify the normal teeth from the pulpitis. The collected images are pre-processed using Histogram Equalization and filtered using Gaussian and Median filters. Modified K-Means algorithm is applied to segment the bony and teeth area from the oral cavity area. Integral Histogram of Gradients with Discrete Wavelet Transform feature extraction techniques and Multi-Layer Neural Network Classifier is proposed to achieve the accuracy of 91.09% which can be used as an assistive tool by the dentist to diagnose pulpitis.

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Gupta, Sonam, Raman Kumar, Ashwarya Sharma, Sachin Jindal, and Gaurav Pandav. "Role of stem cell in treatment of various dental and systemic diseases." Science Progress and Research 1, no. 2 (April 4, 2021): 71–79. http://dx.doi.org/10.52152/spr/2021.114.

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Stem cells are cells that have the ability to continuously divide and produce progeny that differentiate into various other types of cells or types of tissues. In the battle to fight against diseases, stem cells present potencies to repair tissues by cell therapy and tissue regeneration. The study of stem cells turns to be a major frontier in 21th century biology and medicine. Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have been isolated from dental pulp tissue of adult permanent teeth and deciduous teeth, respectively. The present article highlights the origin and class-ification of stem cells with their applications in the field of medicine and dentistry. Despite promising scientific results with genetically modified stem cells, some major problems remain to be overcome. Strategies that combine gene targeting with embryonic stem cellbased therapy are thus potential novel therapeutic options. Further research is essential to determine the full potential of both adult and embryonic stem cells in this exciting new field.

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Sabogal, Ángelo, Jhonn Asencios, Ada Robles, Eloy Gamboa, José Rosas, Jorge Ríos, and Frank Mayta-Tovalino. "Epidemiological Profile of the Pathologies of the Oral Cavity in a Peruvian Population: A 9-Year Retrospective Study of 18,639 Patients." Scientific World Journal 2019 (February 3, 2019): 1–8. http://dx.doi.org/10.1155/2019/2357013.

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Aim. To determine the epidemiological profile of oral diseases in a marginal urban Peruvian population. Methods. A 9-year retrospective study was conducted, analysing 18,639 clinical records from the María Auxiliadora Hospital between 2006 and 2015 with diagnoses of oral lesions using ICD-10 criteria. Clinical records were analysed for sex, tumour, periapical abscess and sinus, cysts of the oral region, other lesions of the oral mucosa and cavity, gingivitis and periodontal disease, dentofacial anomalies, diseases of pulp, etc. Results. Of 18,639 cases, the prevalence was higher in women for the following pathologies: unspecified dental caries (30.6%); impacted tooth in the category of disorders of tooth development and eruption (2.0%); pulpitis (6.8%) in the category of diseases of pulp and periapical tissues; temporomandibular joint (TMJ) disorders (1.5%) in the category of dentofacial anomalies; acute gingivitis (7.5%); radicular cyst (0.3%) in the category of cysts of the oral region; and periapical abscess without sinus (2.0%). Conclusions. We found a significant association between sex and different types of dental caries, disturbances in tooth eruption, diseases of pulp and periapical tissues, and dentofacial anomalies. The study also shows a statistically significant association between sex and gingivitis, periodontal disease, and periodontal abscess and sinus.

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Ostanina, D. A., S. Sh Alimukhamedova, and A. V. Mitronin. "Endodontic-periodontal continuum of tooth: Systematic literature review." Endodontics Today 22, no. 1 (April 4, 2024): 60–65. http://dx.doi.org/10.36377/et-0003.

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INTRODUCTION. The pathogenetic relationship between periodontal and pulp diseases was first described by Simring and Goldberg in 1964. Since then, the term “endodontic-periodontal” lesion (or “endo-perio”) has been used to describe lesions caused by inflammatory products found to varying degrees in both the periodontal and dental pulp tissues. The pulp and periodontium have embryonic, anatomical and functional relationships. The simultaneous presence of changes in pulp tissue and inflammatory periodontal diseases can complicate diagnosis and treatment planning. The etiology, pathogenesis and treatment methods of endodontic-periodontal lesions can vary from quite simple to relatively complex aspects. Knowledge of the basis of concomitant inflammatory processes in the pulp and periodontium is important for making the correct diagnosis.AIM. Carry out a systematic analysis of the literature and summarize the available data on etiopatogenetic relations, diagnostic features and clinical history of endodontic periodontal dental lesions. MATERIAL AND METHODS. A systematic literature analysis was carried out, as well as a manual search in the databases PubMed, Google Search, eLibrary, disserCat, Cyberleninka. Among national and foreign publications corresponding to the topic of this review, 28 publications were selected for systematic analysis, the results of literature review are presented in this article.CONCLUSION. This systematic review found that dental anatomy and the etiology of endodontic periodontal lesions are the basis for difficulties in the diagnosis and treatment of patients with endo-periodontal lesions. Determining the prevalence of infection and the individual characteristics of EPP pathogenesis in patients in this group remains a challenge. In the future, it is necessary to develop new methods of diagnosis, prophylaxis and complex treatment of combined periodontal tissue and tooth pulp, which will improve the prognosis of treatment of injured teeth in the long term.

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Santosh, R., N. Meena, P. Ashwini, Anjali Kaiwar, Ashish Shetty, and DN Naveen. "Effect of Long-term Corticosteroids on Dental Pulp." World Journal of Dentistry 1, no. 1 (2010): 17–20. http://dx.doi.org/10.5005/jp-journals-10015-1004.

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ABSTRACT Corticosteroids are used in the treatment of chronic autoimmune diseases and many long-term illnesses. While these drugs may be considered essential for the patient's health, adverse reactions do occur. The incidence of adverse reactions depends upon the dose level and the duration of treatment. A pilot study was conducted on ten patients on long-term corticosteroids therapy. Pulpal obliteration was assessed by radiographic records. In most instances, pulpal changes to some degree were observed.

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Chandwani, Neelam D., Unnati Devanand Gedam, Ranjana Deshmukh, Darshan M. Dakshindas, and Manish Shrigiriwar. "Mines of cytokine: A treasure trove in pulpal and periapical diseases." Journal of Conservative Dentistry and Endodontics 27, no. 3 (March 2024): 227–32. http://dx.doi.org/10.4103/jcde.jcde_289_23.

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Abstract Pulpitis is a special disease of dental pulp. It causes localized inflammation, due to various inflammatory mediators such as cytokines and chemokines. These inflammatory mediators are responsible for various reparative and resorptive processes in the dental pulp. The balance between these processes ultimately determines the viability of the tooth. Due to the important properties of various inflammatory markers, the correlation of cytokinin gene expression in various stages of inflammation becomes necessary to focus on. Several studies in the past have focused on the importance of such correlation to help in diagnostic applications. The nature of these inflammatory mediators can help us in diagnostic evaluation. Several attempts have been made to focus on these associations so that it can assist in making clinical decisions effectively. The data available are vast but are the most neglected topic. This review article briefly outlines and summarizes the importance of various inflammatory mediators such as cytokinin and chemokines in various pathways of pulpal and periapical inflammation in explanatory and diagrammatic forms. Knowledge gained about pulpal inflammatory response may aid in understanding the molecular level of inflammatory pulpal and periapical diseases, which shall modify our future diagnostic modalities. Several medicaments are used in the treatment of minimal to advanced dental caries which leads to periapical infections. Thorough understanding of these medicaments can resolve secondary infection and can improve the prognosis of the treated tooth.

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Pattan, S. R., P. S. Bykod, P. U. Shetkar, S. V. Pattewar, S. V. Kothiwale, and J. S. Pattan. "SIGNIFICANCE AND NEED OF TREATMENT OF PERIAPICAL LESION." INDIAN DRUGS 51, no. 07 (July 28, 2014): 5–13. http://dx.doi.org/10.53879/id.51.07.p0005.

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Dental caries, one of the most prevalent infectious diseases worldwide, affects approximately 80% of children and the majority of adults. Dental caries may result in endodontic disease, leading to dental pulp necrosis, periapical inflammation and bone desorption, severe pain, and tooth loss. Periapical inflammation may also increase inflammation in other parts of the body. Although many studies have attempted to develop therapies for this disease, there is still an urgent need for effective treatments.

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Todorovic, Vera, Dejan Markovic, Nadezda Milosevic-Jovcic, Marijana Petakov, Bela Balint, Miodrag Colic, Ana Milenkovic, Ivana Colak, Vukoman Jokanovic, and Nebojsa Nikolic. "Dental pulp stem cells: Potential significance in regenerative medicine." Serbian Dental Journal 55, no. 3 (2008): 170–79. http://dx.doi.org/10.2298/sgs0803170t.

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To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages.

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Maganur, Prabhadevi. "Dental Pulp Stem Cells in Regenerative Therapy." TEXILA INTERNATIONAL JOURNAL OF ACADEMIC RESEARCH 10, no. 2 (April 28, 2023): 70–77. http://dx.doi.org/10.21522/tijar.2014.10.02.art007.

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Stem cells, also known as progenitor/precursor cells, have the unique trait of self-renewal and multi-lineage differentiation. Dental stem cells (DSCs) are holding a pivotal role during recent times as they thrive as the cornerstone for the development of cell transplantation therapies that correct periodontal disorders and damaged dentin. DSCs are used therapeutically for different organ systems and numerous diseases, including neurological disorders, diabetes, liver disease, bone tissue engineering, and dentistry. In dentistry, the focus is on predominantly regenerating the pulp and damaged dentin, repairing perforations, and periodontal regenerations. Above all, whole tooth regeneration has been constantly under research. The next decade could be a crucial junction where huge leaps in stem cell-based regenerative therapies could become a reality with successful tissue engineering therapies this could be a biological alternative to synthetic materials that are in use currently. But dental stem cells have their share of challenges for which the research must happen effectively adhering to social responsibilities at all levels. Keywords: Stem cells, Regeneration, Regenerative therapy, SHED.

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Luo, Lihua, Yan He, Xiaoyan Wang, Brian Key, Bae Hoon Lee, Huaqiong Li, and Qingsong Ye. "Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair." Stem Cells International 2018 (2018): 1–15. http://dx.doi.org/10.1155/2018/1731289.

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This review summarizes current advances in dental pulp stem cells (DPSCs) and their potential applications in the nervous diseases. Injured adult mammalian nervous system has a limited regenerative capacity due to an insufficient pool of precursor cells in both central and peripheral nervous systems. Nerve growth is also constrained by inhibitory factors (associated with central myelin) and barrier tissues (glial scarring). Stem cells, possessing the capacity of self-renewal and multicellular differentiation, promise new therapeutic strategies for overcoming these impediments to neural regeneration. Dental pulp stem cells (DPSCs) derive from a cranial neural crest lineage, retain a remarkable potential for neuronal differentiation, and additionally express multiple factors that are suitable for neuronal and axonal regeneration. DPSCs can also express immunomodulatory factors that stimulate formation of blood vessels and enhance regeneration and repair of injured nerve. These unique properties together with their ready accessibility make DPSCs an attractive cell source for tissue engineering in injured and diseased nervous systems. In this review, we interrogate the neuronal differentiation potential as well as the neuroprotective, neurotrophic, angiogenic, and immunomodulatory properties of DPSCs and its application in the injured nervous system. Taken together, DPSCs are an ideal stem cell resource for therapeutic approaches to neural repair and regeneration in nerve diseases.

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Näsström, Karin, Björn Forsberg, Arne Petersson, and Per-Lennart Westesson. "Narrowing of the dental pulp chamber in patients with renal diseases." Oral Surgery, Oral Medicine, Oral Pathology 59, no. 3 (March 1985): 242–46. http://dx.doi.org/10.1016/0030-4220(85)90159-8.

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Zayed, Mohammed, and Koichiro Iohara. "Effects of p-Cresol on Senescence, Survival, Inflammation, and Odontoblast Differentiation in Canine Dental Pulp Stem Cells." International Journal of Molecular Sciences 21, no. 18 (September 21, 2020): 6931. http://dx.doi.org/10.3390/ijms21186931.

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Aging, defined by a decrease in the physical and functional integrity of the tissues, leads to age-associated degenerative diseases. There is a relation between aged dental pulp and the senescence of dental pulp stem cells (DPSCs). Therefore, it is important to investigate the molecular processes underlying the senescence of DPSCs to elucidate the dental pulp aging mechanisms. p-Cresol (PC), a uremic toxin, is strongly related to cellular senescence. Here, age-related phenotypic changes including senescence, apoptosis, inflammation, and declining odontoblast differentiation in PC-treated canine DPSCs were investigated. Under the PC condition, cellular senescence was induced by decreased proliferation capacity and increased cell size, senescence-associated β-galactosidase (SA-β-gal) activity, and senescence markers p21, IL-1β, IL-8, and p53. Exposure to PC could stimulate inflammation by the increased expression of IL-6 and cause the distraction of the cell cycle by the increased level of Bax protein and decreased Bcl-2. The levels of odontoblast differentiation markers, dentin sialophosphoprotein (DSPP), dentin matrix protein 1, and osterix, were decreased. Consistent with those findings, the alizarin red staining, alkaline phosphatase, and DSPP protein level were decreased during the odontoblast differentiation process. Taken together, these findings indicate that PC could induce cellular senescence in DPSCs, which may demonstrate the changes in aging dental pulp.

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Min, Qi, Liqiong Yang, Hua Tian, Lu Tang, Zhangang Xiao, and Jing Shen. "Immunomodulatory Mechanism and Potential Application of Dental Pulp-Derived Stem Cells in Immune-Mediated Diseases." International Journal of Molecular Sciences 24, no. 9 (April 29, 2023): 8068. http://dx.doi.org/10.3390/ijms24098068.

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) derived from dental pulp tissue, which have high self-renewal ability and multi-lineage differentiation potential. With the discovery of the immunoregulatory ability of stem cells, DPSCs have attracted much attention because they have similar or even better immunomodulatory effects than MSCs from other sources. DPSCs and their exosomes can exert an immunomodulatory ability by acting on target immune cells to regulate cytokines. DPSCs can also migrate to the lesion site to differentiate into target cells to repair the injured tissue, and play an important role in tissue regeneration. The aim of this review is to summarize the molecular mechanism and target cells of the immunomodulatory effects of DPSCs, and the latest advances in preclinical research in the treatment of various immune-mediated diseases, providing new reflections for their clinical application. DPSCs may be a promising source of stem cells for the treatment of immune-mediated diseases.

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Drancourt, Michel, Gérard Aboudharam, Olivier Croce, Fabrice Armougom, Catherine Robert, and Didier Raoult. "Dental pulp as a source of low-contaminated DNA." Microbial Pathogenesis 105 (April 2017): 63–67. http://dx.doi.org/10.1016/j.micpath.2017.02.007.

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Wei, Yali, Ping Lyu, Ruiye Bi, Xinyu Chen, Yanshen Yu, Zucen Li, and Yi Fan. "Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends." International Journal of Molecular Sciences 23, no. 24 (December 7, 2022): 15492. http://dx.doi.org/10.3390/ijms232415492.

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Pulpal and periapical diseases are the most common dental diseases. The traditional treatment is root canal therapy, which achieves satisfactory therapeutic outcomes—especially for mature permanent teeth. Apexification, pulpotomy, and pulp revascularization are common techniques used for immature permanent teeth to accelerate the development of the root. However, there are obstacles to achieving functional pulp regeneration. Recently, two methods have been proposed based on tissue engineering: stem cell transplantation, and cell homing. One of the goals of functional pulp regeneration is to achieve innervation. Nerves play a vital role in dentin formation, nutrition, sensation, and defense in the pulp. Successful neural regeneration faces tough challenges in both animal studies and clinical trials. Investigation of the regeneration and repair of the nerves in the pulp has become a serious undertaking. In this review, we summarize the current understanding of the key stem cells, signaling molecules, and biomaterials that could promote neural regeneration as part of pulp regeneration. We also discuss the challenges in preclinical or clinical neural regeneration applications to guide deep research in the future.

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Diana, Sherli, and Isyana Erlita. "PREVALENCE OF DISEASE IN THE CONSERVATIVE DENTISTRY DEPARTMENT OF GUSTI HASAN AMAN DENTAL HOSPITAL BANJARMASIN IN 2015-2018 (RETROSPECTIVE STUDY)." Dentino : Jurnal Kedokteran Gigi 5, no. 2 (August 15, 2020): 201. http://dx.doi.org/10.20527/dentino.v5i2.8975.

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ABSTRACTBackground: The most common disease in society is dental problems. People often care less about oral health. Dental hard tissue disease is considered as a mild disease, not contagious and not life-threatening, so people will visit the dentist if they feel disturbed, especially in terms of mastication, swallowing and speaking. Dental hard tissue diseases in conservative dentistry that often causes problems are reversible pulpitis, irreversible pulpitis, and pulp necrosis. Until now, there are no definitive data on the prevalence of dental hard tissue disease in conservative dentistry in Indonesia, as well as data on the prevalence of dental hard tissue disease in Banjarmasin, South Kalimantan. Purpose: To find out the prevalence of hard tissue disease in conservative dentistry in patients who came to Gusti Hasan Aman Dental Hospital, Banjarmasin, South Kalimantan in 2015-2018. Methods: This research was a descriptive study through a retrospective study. Samples in this study were taken by purposive sampling method, totaling 11,480 medical records of patients who came to the Conservative Dentistry Department at Gusti Hasan Aman Dental Hospital Banjarmasin in 2015-2018. Results: The data obtained showed the prevalence of reversible pulpitis as many as 6,700 (59%), irreversible pulpitis as many as 1,291 (11%), pulp necrosis of 3,234 people (28%), and others as many as 255 people (2%). Conclusion: The highest prevalence of dental hard tissue disease in the Conservative Dentistry Department of Gusti Hasan Aman Dental Hospital in 2015-2018 is reversible pulpitis, followed by pulp necrosis, and irreversible pulpitis. Keywords: Necrosis, Prevalence, Pulpitis,

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Pindobilowo, Dwi Ariani, and Dewi Puspitasari. "Use of Nanoparticles as Materials to Prevent Dental and Oral Diseases." Formosa Journal of Applied Sciences 3, no. 4 (April 30, 2024): 2123–34. http://dx.doi.org/10.55927/fjas.v3i4.8795.

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Introduction: Nanoparticles can be applied in various fields of dentistry such as preventive dentistry, dental caries, periodontal disease, restorations, pulp and periapical lesions, oral candidiasis, denture stomatitis, hyposalivation, and head, neck and oral cancer. The purpose of this paper is to determine the uses and benefits of nanoparticles in the field of dentistry. Methodology: using the Narrative Review method based on journal databases including: PubMed, EBSCO, ResearchGate, and ProQuest from 2016 to 2022. Conclusion: Beneficial nanoparticles can improve the diagnosis, prevention, and treatment of various oral diseases, including dental caries, periodontal disease, lesions pulpal and periapical, oral candidiasis, denture stomatitis, hyposalivation, and head, neck, and oral cancer.

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S., Nachiappan, Ajay Chandran, Swathika B., Ganesan S., Mahaprasad A., Farahnaz Muddebihal, and Abhishek Singh Nayyar. "Pulp Stones: Diagnostic Significance in Early Diagnosis and Radiographic Correlation with Ischemic Heart Diseases." Indian Journal of Radiology and Imaging 31, no. 02 (April 2021): 277–83. http://dx.doi.org/10.1055/s-0041-1731829.

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Abstract Context and Aim Sufficient evidence exists in the literature which indicates that patients with ischemic heart diseases (IHDs) show higher degree of pulp calcifications. The present study was, therefore, planned to estimate the prevalence of pulp stones in patients diagnosed with/or undergoing treatment for IHDs. Materials and Methods The present study, which consisted of 300 subjects within the age range of 25 to 65 years, was divided into two groups: study group comprising 150 patients (113 males and 37 females) and 150 age- and sex-matched healthy controls. Pulp stones were imaged using bitewing radiographs and paralleling technique under standard conditions. Statistical Analysis Used Statistical analysis was done using IBM SPSS Statistics for Windows version 21 (IBM Corp, Armonk, USA), while independent t-test and Chi-square test were done to check the prevalence of pulp stones in the study and control groups, based on gender-, arch-, region- and side-wise distribution. p < 0.05 was considered statistically significant. Results The patients with IHDs exhibited 100% prevalence of pulp stones and the difference was found to be statistically significant, although there was a significant difference in the mean number of pulp stones observed in the study and control groups, with the study group revealing 2217 pulp stones as against 639 pulp stones observed in the control group (p < 0.001). Furthermore, maxillary arch and posterior teeth were predominantly affected in both the groups, although the difference was not found to be statistically significant. Conclusions The findings of the present study suggested a positive correlation between pulp stones and IHDs, highlighting the significance of dental radiographic examination, which may have a possibility for an early detection of IHDs.

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Matos, Felipe de Souza, Thaís Christina Cunha, Maria Amália Gonzaga Ribeiro, Camila Silva Araujo, Ítalo de Macedo Bernardino, Camilla Christian Gomes Moura, and Luiz Renato Paranhos. "Accuracy of the dental pulp sensibility test using cold spray for the diagnosis of pulp diseases: an observational clinical study." Bioscience Journal 37 (July 26, 2021): e37040. http://dx.doi.org/10.14393/bj-v37n0a2021-60365.

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The dental pulp sensibility test is one of the main auxiliary resources for the diagnosis of pulp pathologies, and its accuracy is still debatable. This cross-sectional observational study evaluated the accuracy of the pulp sensibility test (PST) using cold spray (1,1,1,2-tetrafluoroethane) for the diagnosis of pulp diseases and determined the effect of individual and clinical variables on the reliability of this test. The paper was designed following the STROBE statement. Sixty patients with indications for primary endodontic treatment were selected and examined from August 2017 to July 2018. Data collection was performed through interviews, clinical/radiographic examinations and the PST. The results of the cold test, along with data on sex, age, the tooth type regarding the root number, and the presence of restorations and caries, as well as the recent consumption of analgesics, were recorded. The presence of bleeding within the pulp chamber was used as the gold standard to compare with the clinical diagnosis and to identify the true-positive, false-positive, true-negative, and false-negative responses. The accuracy of PST achieved in subgroups of individual and clinical variables was compared using the chi-square test with a significance level of 5% (p < 0.05). The PST with the use of cold spray showed a sensitivity of 0.88, a speciﬁcity of 1.00, a positive predictive value of 1.00, a negative predictive value of 0.86, and an accuracy of 0.93. The accuracy of the cold spray was not affected by individual or clinical variables. The PST with the use of cold spray is an accurate and reliable method for determining the diagnosis of pulp diseases, especially in cases of pulp vitality or irreversible pulpitis.

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Zhang, Qian, Xing Huang, Yihong Xiu, Yaping Quan, Huojia Muhetaer, and Tao Liu. "Src Homology 2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) Combined with Dental Pulp Stem Cells Promote the Effect of Angiogenesis." Journal of Biomaterials and Tissue Engineering 12, no. 7 (July 1, 2022): 1403–10. http://dx.doi.org/10.1166/jbt.2022.3040.

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Pulpitis is one of the most important dental diseases. How to improve the blood circulation in the infected and necrotic area of pulp is the current research hotspot in the treatment of pulpitis. Mesenchymal stem cells (MSCs) have similar regeneration and differentiation ability to the pluripotent stem cells, and can differentiate into various tissues under certain induced conditions. Dental pulp stem cells (DPSCs) extracted from dental pulp, which have stronger proliferation ability and stability, and are more ideal seed cells for the treatment of pulpitis. Research show that Src homology 2 domain containing SHP2 can promote blood vessel growth. In this subject, we studied the angiogenesis of SHP2 combined with dental pulp stem cells (DPSCs) transplantation. SHP2 and DPSCs were co cultured with human umbilical vein endothelial cells (HUVECs). The proliferation and migration of endothelial cells were detected by Wound Healing Assays. At the same time, the effect of SHP2+DPSC on endothelial cell angiogenesis was examined by tube formation test. The expression of angiogenesis related cytokines including vascular endothelial growth factor (VEGF), von willebrand factor (vWF), Angiopoietin-1 (Ang-1) and Cdc42/Rac1 signal pathway were also detected by Western blot. Our results demonstrated that SHP2 combined with DPSCs can advance endothelial cell angiogenesis. Meanwhile, SHP2+ DPSC obviously increased VEGF, Ang-1 and vWF expression. SHP2+DPSC significantly raise the Cdc42/Rac1 signal pathway in HUVECs. Our data illustrate that SHP2 combined with DPSCs can promote the effect of angiogenesis in pulpitis.

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Gabiec, K., K. Wyrzykowska, Z. Dąbrowska, M. Antoniak, and E. Dąbrowska. "Stem cells are the hope of modern stomatology." Progress in Health Sciences 7, no. 1 (March 16, 2017): 0. http://dx.doi.org/10.5604/01.3001.0010.1880.

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Introduction: Stem cells are often hailed as the medicine of the 21st century. They provide us with potential tools to effectively counteract not only diseases, but even aging. For stomatology, stem cells are the technology of the future in the regeneration of the periodontium and pulp, and dental replantation and transplantation. Materials and methods: On the basis of a literature review, the previous achievements and potential capabilities of stem cell therapy were discussed, focusing on dental applications. Conclusions: The paper discusses the modulation of stem cells and their therapeutic potential and capabilities. The presence and properties of stem cells in the pulp of human deciduous and permanent teeth, the periodontal membrane and the dental sac are also discussed. The results of the studies conducted by the cited researchers are promising and give hope for the development of regenerative and restorative processes of the dental and periodontal tissues. Summary: In the future, stem cells obtained from primary and permanent teeth deposited in special dental banks will be able to prevent the degradation of periodontal tissue, or even heal the teeth.

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Utami, Istri Dwi, Farina Pramanik, and Lusi Epsilawati. "Proporsi gambaran radiografis lesi periapikal gigi nekrosis pada radiograf periapikalRadiographic image proportion of necrotic teeth periapical lesions on periapical radiographs." Padjadjaran Journal of Dental Researchers and Students 3, no. 1 (July 12, 2019): 64. http://dx.doi.org/10.24198/pjdrs.v3i1.22306.

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Pendahuluan: Karies merupakan penyakit yang paling banyak terjadi. Pada tahun 2013 kerusakan gigi masyarakat Indonesia adalah 460 buah gigi per 100 orang. Jika dibiarkan tidak dirawat akan berkembang mengarah pada kematian pulpa dan akan menyebar menyebabkan infeksi periapikal. Tahun 2010 penyakit pulpa dan periapikal menempati posisi ke 7 dari 10 penyakit terbanyak pada pasien rawat jalan di rumah sakit di Indonesia. Teknik radiograf yang dapat digunakan untuk diagnosis penyakit pulpa dan periapikal adalah teknik radiografi periapikal. Tujuan penelitian adalah mendapatkan informasi mengenai proporsi gambaran radiografis lesi periapikal gigi nekrosis di RSGM Unpad. Metode: Jenis penelitian deskriptif. Populasi penelitian adalah seluruh radiograf periapiakal gigi nekrosis dengan lesi periapikal pada bulan November 2018 – Januari 2019 di Instalasi Radiologi Kedokteran Gigi RSGM Unpad. Sampel penelitian ditentukan dengan metode purposive sampling. Jumlah sampel sebanyak 54 radiograf periapikal. Hasil: Proporsi gambaran radiografis lesi periapikal yaitu abses periapikal sebanyak 42 kasus (77,78%), granuloma periapikal 8 kasus (14,81%) dan kista periapikal 4 kasus (7,40 %). Simpulan: Proporsi gambaran radiografis lesi periapikal gigi nekrosis di RSGM Unpad didapatkan proporsi tertinggi adalah abses periapikal diikuti granuloma periapikal dan yang terakhir adalah kista periapikal.Kata Kunci: Gigi nekrosis, lesi periapikal, radiograf periapikal ABSTRACTIntroduction: Caries is the most common disease. In 2013, tooth decay of Indonesian people was 460 teeth per 100 people. If left untreated, it will develop, leading to pulp death and will spread, causing periapical infection. In 2010, pulp and periapical diseases were ranked 7th out of the ten most diseases in outpatients of the hospitals in Indonesia. A radiographic technique that can be used for the diagnosis of pulp and periapical disease is a periapical radiographic technique. This study was aimed to obtain information about the radiographic image proportion of necrotic teeth periapical lesions at Universitas Padjadjaran Dental Hospital. Methods: This study was descriptive, with study population was all radiographs of the necrotic teeth periapical lesions in November 2018 - January 2019 at Dentomaxillofacial Radiology Installation of Universitas Padjadjaran Dental Hospital. The research sample was determined by purposive sampling method. The number of samples was 54 periapical radiographs. Results: The radiographic image proportion of necrotic teeth periapical lesions, namely periapical abscesses in 42 cases (77.78%), periapical granuloma in 8 cases (14.81%) and periapical cysts in 4 cases (7.40%). Conclusion: The radiographic image proportion of necrotic teeth periapical lesions at Universitas Padjadjaran Dental Hospital mostly are periapical abscesses, followed by periapical granuloma, and the least is periapical cysts.Keywords: Necrotic teeth, periapical lesions, periapical radiographs

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Picos, Andrei, Andreea L. Rachisan, and Alexandra Dadarlat. "Minimally Invasive Dental Treatment Using Composites and Ceramics in GERD Diagnoses Patients." Materiale Plastice 55, no. 2 (June 30, 2018): 252–54. http://dx.doi.org/10.37358/mp.18.2.5004.

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Specialty literature demonstrates the direct link between gastro-esophageal reflux diseases (GERD) and dental errosion (DE). Patients diagnosed with GERD often shown dental tissue loss in the enamel and dentine, somentimes going as far as opening the pulp chamber. Modern minimal invasive treatments make use of the current performance of cements and prosthetic materials (composites and ceramics) to conserve healthy enamel and dentine, in comparison to classic reconstruction methods.

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Bar, Julia K., Anna Lis-Nawara, and Piotr Grzegorz Grelewski. "Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential." International Journal of Molecular Sciences 22, no. 21 (November 6, 2021): 12018. http://dx.doi.org/10.3390/ijms222112018.

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The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting of various biomolecules, is undergoing intense research. Despite promising in vitro and in vivo studies, most DSC secretome-based therapies have not been implemented in human medicine because the paracrine effect of the bioactive factors secreted by human dental pulp stem cells (hDPSCs) and human exfoliated deciduous teeth (SHEDs) is not completely understood. In this review, we outline the current data on the hDPSC- and SHED-derived secretome as a potential candidate in the regeneration of bone, cartilage, and nerve tissue. Published reports demonstrate that the dental MSC-derived secretome/conditional medium may be effective in treating neurodegenerative diseases, neural injuries, cartilage defects, and repairing bone by regulating neuroprotective, anti-inflammatory, antiapoptotic, and angiogenic processes through secretome paracrine mechanisms. Dental MSC-secretomes, similarly to the bone marrow MSC-secretome activate molecular and cellular mechanisms, which determine the effectiveness of cell-free therapy. Many reports emphasize that dental MSC-derived secretomes have potential application in tissue-regenerating therapy due to their multidirectional paracrine effect observed in the therapy of many different injured tissues.

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Kim, Hong Bae, Ku Youn Baik, Moon Ho Kang, and Jong Hoon Chung. "Effects of Photobiomodulation Using Near-Infrared Light on the Dentin and Periodontal Ligament in a Beagle Model." Applied Sciences 14, no. 2 (January 15, 2024): 724. http://dx.doi.org/10.3390/app14020724.

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In this study, we investigated the effect of photobiomodulation (PBM) using near-infrared light on the dentin and periodontal ligament in a beagle model. We utilized a specific PBM device to irradiate NIR light with a wavelength of 810 nm and an energy density of 80.22 mJ/cm2. The device’s settings were optimized for a frequency of 300 Hz and a 30% duty cycle, allowing precise and controlled light exposure. Through a comprehensive analysis involving micro-computed tomography, scanning electron microscopy, and hematoxylin and eosin staining, we demonstrated increased odontoblast activity at the pulp–dentin interface in PBM-treated samples. This increased activity may be postulated to potentially contribute to alleviating dental hypersensitivity through the differentiation of dental pulp stem cells and the promotion of vascular development within the odontoblast layer. Moreover, our observations also indicated an improvement in the strength and integrity of fibrous connective tissue within the periodontal ligament. These findings highlight the potential of PBM with specific parameters applied using NIR as a valuable treatment method for tooth tissue regeneration. It shows particular promise in the treatment of dental diseases associated with dentin and periodontal ligament damage and offers a new perspective in the management of tooth hypersensitivity and other related dental diseases.

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Janowicz, Krzysztof, Paul Mozdziak, Artur Bryja, Bartosz Kempisty, and Marta Dyszkiewicz-Konwińska. "Human Dental Pulp Stem Cells: recent findings and current research." Medical Journal of Cell Biology 7, no. 3 (November 8, 2019): 119–24. http://dx.doi.org/10.2478/acb-2019-0016.

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AbstractPrevalence of neurodegenerative diseases, most of which are life threatening and incurable, is an increasing clinical problem. To date, studies have demonstrated a superior proliferation rate of dental pulp stem cells (DPSCs) compared to other mesenchymal stem cells in vitro. DPSCs has recently been recognized as a novel treatment strategy for neurodegenerative disease, due to their advanced potential for neurogenic differentiation. The oral cavity has been described as a promising source of dental pulp stem cells. DPSCs are widely used in regenerative dentistry holding alternative capacity for osteogenic differentiation and therefore new promises for tissue and whole tooth regeneration. Dental stem cell banking offers a plentiful source of stem cells representing great potential for cell reprogramming and thus cell therapy. Recently, the association of pulp stem cells with three – dimensional scaffold templates allows for building up naturally derived implants. This review introduces to unique properties of DPSCs and biological factors influencing mineralization, proliferation and differentiation of pulp stem cells. Latest research studies are compared in terms of effectiveness and limitations of techniques for the isolation of pulp stem cells, including the enzymatic digestion and the explant culture methods. Moreover, a short overview of most recent findings and clinical application of DPSCs is proffered including progress of current research and limitations still to be addressed in the nearest future. Finally, the article presents new advances in the area of regenerative dentistry and regenerative medicine, including three dimensional printing and three dimensional analysis, emerged to deepen studies under procedures to replace the non patient specific artificial implants.Running title: DPSCs - review

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Journal articles on the topic 'Dental Pulp Diseases'

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