Relevant bibliographies by topics / Detection PCR real-time

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  3. Books
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Academic literature on the topic 'Detection PCR real-time'

Author: Grafiati
Published: 9 March 2023

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Journal articles on the topic "Detection PCR real-time"

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KAMJOO, Maryam NASER, and Ali NAZEMI. "VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING." / International Journal of Health Services Research and Policy 1, no. 1 (January 29, 2016): 15–19. http://dx.doi.org/10.23884/ijhsrp.2016.1.1.02.

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Kang, Won, Sang-Bum Park, Youn-Hyoung Nam, Young-Chang An, Sang-Hyun Lee, Won-Cheoul Jang, Su-Min Park, Jong-Wan Kim, and Song-Chun Chong. "Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods." Journal of the Korean Chemical Society 51, no. 1 (February 20, 2007): 36–42. http://dx.doi.org/10.5012/jkcs.2007.51.1.036.

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Navarro, E., G. Serrano-Heras, M. J. Castaño, and J. Solera. "Real-time PCR detection chemistry." Clinica Chimica Acta 439 (January 2015): 231–50. http://dx.doi.org/10.1016/j.cca.2014.10.017.

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Gospodinović, Hristina, Ljiljana Pavlović, Marija Obradović, Sanja Dimitrijević, Sofija Jovanović, and Edita Grego. "Detection of high-risk HPV genotypes using Real-time PCR." Glasnik javnog zdravlja 96, no. 4 (2022): 416–26. http://dx.doi.org/10.5937/serbjph2204416g.

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Discovery of the causal relationship between the human papilloma virus and cervical cancer formation increased the significance of the real-time PCR in HPV diagnostics. Based on evidence showing that they caused cervical cancer, 14 HPV types have been classified as carcinogenic (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). This study analysed cervical smears taken from female patients, aged 19 to 25 years, using the Viasure diagnostic test for the detection of high-risk HPV genotypes and individual identification of HPV genotypes 16 and 18. A total of 110 cervical smears were analysed and 44 positive samples were detected (40%). DNA analysis of the positive samples found the following distribution of the HPV types: 27% HPV (31, 39, 56); 22% HPV (52, 59, 68); 18% HPV16; 13% HPV (33, 45, 51); 12% HPV (35, 58, 66); 8% HPV18. This study and the high positivity rate it found indicate that there is a lack of awareness among the youth on the measures of prevention, as well as a lack of understanding of HPV infection.
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Abdulina, D. R. "DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR." Biotechnologia Acta 13, no. 2 (April 2020): 38–47. http://dx.doi.org/10.15407/biotech13.02.038.

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Poltronieri, P., M. D. de Blasi, and D. Urso OF. "Detection of Listeria monocytogenes through real-time PCR and biosensor methods." Plant, Soil and Environment 55, No. 9 (October 14, 2009): 363–69. http://dx.doi.org/10.17221/139/2009-pse.

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<I>Listeria monocytogenes</I> is a foodborne pathogen causing listeriosis, especially in sensitive individuals such as children, pregnant women and persons with compromised immune systems. This pathogen has been isolated from different food products, but milk products surely play a major role in the epidemiology of this foodborne disease. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of <I>Listeria</I> spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard, but in the last years more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid sequence-based amplification (NASBA). In this review, real-time PCR assays, protein chip methods and label-free SPR immunosensors were compared for their application in bacterial detection.
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Ahrberg, Christian D., Bojan Robert Ilic, Andreas Manz, and Pavel Neužil. "Handheld real-time PCR device." Lab on a Chip 16, no. 3 (2016): 586–92. http://dx.doi.org/10.1039/c5lc01415h.

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World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use in small clinics and point-of-care applications.
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Nitsche, Andreas, Mathias Büttner, Sonja Wilhelm, Georg Pauli, and Hermann Meyer. "Real-Time PCR Detection of Parapoxvirus DNA,." Clinical Chemistry 52, no. 2 (February 1, 2006): 316–19. http://dx.doi.org/10.1373/clinchem.2005.060335.

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Abstract Background: Detection of parapoxviruses is important in various animals as well as in humans as zoonotic infections. Reliable detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, for both veterinarians and medical practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses has been reported. Methods: A minor groove binder–based quantitative real-time PCR assay targeting the B2L gene of parapoxviruses was developed on the ABI Prism and the LightCycler platforms. Results: The real-time PCR assay successfully amplified DNA fragments from a total of 41 parapoxvirus strains and isolates representing the species orf virus, bovine papular stomatitis virus, pseudocowpoxvirus, and sealpoxvirus. Probit analysis gave a limit of detection of 4.7 copies per assay (95% confidence interval, 3.7–6.8 copies per reaction). Scabs contain a sufficient amount of parapoxvirus DNA and can therefore be used for PCR without any DNA preparation step. No cross-reactivity to human, bovine, or sheep genomic DNA or other DNA viruses, including orthopoxviruses, molluscum contagiosum viruses, and yaba-like disease viruses, was observed. Conclusion: The presented assay is suitable for the detection of parapoxvirus infections in clinical material of human and animal origin.
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MENDOZA-ROMERO, LUIS, EDWARD L. C. VERKAAR, PAUL H. SAVELKOUL, ARNOLD CATSBURG, HENK J. M. AARTS, JAAP B. BUNTJER, and JOHANNES A. LENSTRA. "Real-Time PCR Detection of Ruminant DNA." Journal of Food Protection 67, no. 3 (March 1, 2004): 550–54. http://dx.doi.org/10.4315/0362-028x-67.3.550.

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To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.
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Mullah, Bashar, Paul Wyatt, Junko Stevens, Alex Andrus, and Kenneth J. Livak. "Automated real-time PCR detection and quantitation." Collection of Czechoslovak Chemical Communications 61, s1 (1996): 287–89. http://dx.doi.org/10.1135/cccc1996s287.

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Dissertations / Theses on the topic "Detection PCR real-time"

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Malatji, Dikeledi Petunia. "Detection of Babesia rossi genotypes using real-time PCR." Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/31138.

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Babesia rossi is the most virulent Babesia species in dogs occurring in South Africa and is associated with severe clinical manifestation and mortalities. Babesia rossi is highly pathogenic and reacting dogs requires treatment to prevent mortalities. Mild, uncomplicated forms of the disease are effectively treated with antibabesial drugs. Complicated forms of the disease are difficult to treat with high mortality rate.This results in the disease being of economic importance in South Africa. There is a lack of information regarding the relationship between parasite genotype and disease phenotype. The broad objective of this study is to detect B. rossi genotypes using real-time PCR. This test is a method that can be used to monitor amplicon formation throughout the PCR reaction and to estimate the initial concentration of target DNA in samples. Polymerase chain reaction, sequencing and phylogenetic analysis were used to determine the genotype detection of Babesia rossi isolated from infected dogs in South Africa. The correlation between the parasite genotype and disease phenotype was also investigated. Correlation between B. rossi Erythrocyte Membrane Antigen (BrEMA1) genotypes and age of dogs was studied in 44 cases. A total of 101 blood samples were tested using the reverse line blot (RLB) assay. Ninety six percent hybridized to the Babesia rossi species-specific probe. Our findings demonstrate that the most encountered (BrEMA1) genotype is genotype29, followed by genotype28 and genotype19, with genotype29 associated with most of the severe clinical signs diagnosed compared to genotype19. The number of cases caused by genotype19 was low, constituting 22% of the cases. This is comparable with the 2009 report where genotype19 appeared highly prevalent and virulent, whereas the prevalence of genotype28/29 appeared moderate. In this dissertation, we present the first report on the detection of an amplification product of BrEMA1 genotypes using real-time PCR. Samples which were below the detectable limit of conventional PCR and could not be sequenced probably due to low parasitaemia were also used and real-time PCR provided the ability to detect B. rossi positive animals. This was able to detect 10 BrEMA1 genotypes. However, it was not reliable enough in differentiating between various BrEMA1 genotypes. When evaluating the relationship between BrEMA1 genotypes, clinical manifestation and age of the dogs, collapse was found to be a poor prognostic sign in dogs with babesiosis. Genotype29 was associated with most of the collapsed cases and with high number of the dogs that died. Although B. rossi can infect dogs of all ages, young dogs showed to be more susceptible to canine babesiosis than older dogs. This is in agreement with the survey carried out from the Onderstepoort Veterinary Academic Hospital (OVAH) in 1994. Since B. rossi is the most pathogenic species of the large babesias of dogs, the ability to manage the disease is dependent on rapid detection of the organism. Real-time PCR test is indeed a quicker method to confirm diagnoses of B. rossi infected dogs. It can detect B. rossi infection at a low DNA concentration (0.185 ng/μl) which provides a major advantage in detecting B. rossi infection in field blood samples.
Dissertation (MSc)--University of Pretoria, 2011.
Veterinary Tropical Diseases
MSc
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Landgraf, Maria. "Detection of food relevant filamentous fungi by real time PCR." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=98023946X.

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Afshari, Kashanian Elisa. "Detection of celery (Apium graveolens) in food with Real-Time PCR." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7130.

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Directive EC 2003/89/EC of the European Parliament and of the Council states that certain

ingredients and products derived there of known to cause allergen reactions must always be

declared. Furthermore labelling is mandatory irrespective of the amount included. The National

Food Administration therefore needs methods for monitoring the presence of allergens in food.

Methods already exist for most of the allergens on the EU-list, but an operational method for

celery (Apium graveolens) is missing.

A specific DNA-method was developed, based on TaqMan Real-Time PCR with the celery

mannitol dehydrogenase gene as target sequence. The analysis was started with homogenisation

of the sample followed by extraction of DNA. The Real-Time PCR method was shown to be

specific for celery, producing a 113 bp fragment with two celery varieties and negative results

with other closely selected species commonly present together with celery in food products (12

samples). The detection limit was 2-20 pg DNA, which corresponds to 1-7 haploid genome

copies. When evaluated with model samples of celery in meat, a detection limit of less than

0,01 % was determined. When used to analyse food products from the market, six out of seven

products declared to contain celery were correctly identified as positive.

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Muradrasoli, Shaman. "Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays." Doctoral thesis, Uppsala universitet, Klinisk virologi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9193.

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As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). In papers I & II, broadly targeted and specific QRT-PCRs were used to study expression of endogenous and exogenous betaretrovirus sequences in human tissues. Results from human tissues demonstrated endogenous betaretrovirus expression in a tissue-specific manner, highest in reproductive tissues. Despite the high sensitivity, no exogenous betaretrovirus was found in human breast cancer samples. The limits of primer and probe degeneracy for detection of a diverse set of retroviral sequences was evaluated. These methods are useful for further investigations on the pathophysiological contribution(s) of endogenous betaretrovirus and to investigate whether an exogenous betaretrovirus is involved in human breast cancer. In papers III & IV, we developed and applied broadly targeted one-step QRT-PCRs for influenza viruses and coronaviruses. In addition to the generic primers, two novel probe design strategies were used in order to be able to broadly amplify these diverse sets of viruses: A triplex system for simultaneous detection and quantification of influenza A, B and C (3QRT-PCR and further developed 3QRT-PCR-MegB; where MegB stands for MegaBeacon) based on TaqMan® and MegB probes, and a pan-CoV QRT-PCR, based on three TaqMan® probes i.e., degeneracy was distributed on three probes. Probe fault tolerance was thus increased in two ways, either with short probes with/without locked nucleic acid (LNA) nucleotides concentrated to conserved stretches, or with long probes (MegB), compensating mismatching positions with many matching ones. Clinical samples, negative by antigen detection with immunofluorescence (IFA), were influenza A positive with 3QPCR-MegB. Avian pooled samples, negative with an earlier pan-CoV QPCR, came out positive with the triple-probe system. Assay evaluation with clinical samples and reference strains revealed good clinical diagnostic potential. Thus, the thesis describes several strategies to counteract sequence variation of RNA viruses and describes a set of broadly targeted QRT-PCRs useful for scientific screening or diagnostics of betaretroviruses and respiratory viruses.
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Leopold, Luciana Eleanor Dittmer Dirk Peter. "Development of real-time PCR assays for the quantitative detection of herpesviruses." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2008. http://dc.lib.unc.edu/u?/etd,1487.

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Thesis (M.S.)--University of North Carolina at Chapel Hill, 2008.
Title from electronic title page (viewed Sep. 16, 2008). "... in partial fulfillment of the requirements for the degree of Master of Science in the Curriculum of Genetics and Molecular Biology." Discipline: Genetics and Molecular Biology; Department/School: Medicine.
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Elfaitouri, Amal. "Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320.

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Lee, Yu-yan, and 李羽殷. "Detection of influenza C virus in pediatric respiratory specimens by real-time PCR." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/193539.

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Respiratory infection is a major disease burden worldwide. Statistical reports revealed it is one of the main causes of mortality and morbidity especially in young children. Influenza infection is one of the predominant cause associate with respiratory infection. Traditionally, studies have been emphasized on the detection of influenza A and B virus owing to their significance in clinical and economic impact. Attention of influenza C virus is rarely recognized due to its difficulty in isolation. However, recently, increasing reports have been illustrated the co-circulation of influenza C virus globally. Serological studies also suggested majority of people worldwide acquired influenza C virus infection in their early childhood or adolescent stage, yet information regarding influenza C virus is still inadequate. Epidemiological and clinical impact of influenza C virus in pediatric patients in Hong Kong was examined by the approach of real-time PCR. From November 2007 to April 2011, a total of 1, 037 specimens were obtained from pediatric patients exhibited apparent respiratory tract illness in Hong Kong. Eleven strains of influenza C virus were detected by real-time PCR approach. All patients with influenza C virus infection were below 5 years of age with the youngest age of 11 months. The ratio of infection in male to female was approximately one to one. High grade fever appeared to be the most frequent clinical manifestations (10/11) of influenza C virus infection. Upper respiratory tract infection was also occasionally observed. The clinical presentation of influenza C virus was similar to its influenza counterpart. Phylogenetic analysis of influenza C virus was examined in 6/11 of the isolates to determine the lineages of co-circulating influenza C viruses in Hong Kong. Nucleotide sequencing was performed with primer targeting the hemagglutinin-esterase (HE) gene. Result revealed that most of the detected influenza C virus associate with the C/Sao Paulo/378/82 related lineage. Results from this study revealed the positive rate of influenza C was comparable to influenza B and resultant respiratory symptoms could be severe in pediatric patients It is suggested to consider the inclusion of influenza C virus detection in routine diagnostic panel and real-time PCR could be a desirable detection platform account for its sensitivity and rapidity.
published_or_final_version
Microbiology
Master
Master of Medical Sciences
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Chia, Helena Nien-Hwa 1982. "Development of tissue printed nitrocellulose cards/arrays for real time PCR amplification and detection." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/32828.

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Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references (leaf 21).
Tissue print technology allows for the transfer of cellular material from tissue onto a nitrocellulose film for immunocytochemical assays. The MIT BioInstrumentation Laboratory is currently developing a novel cancer marker imaging system for detection of cancerous tissue, which will be useful for discerning tumor margins. This research will advance the recent application of tissue print technology in bio-medicine by combining it with imaging and real time polymerase chain reaction (PCR) amplification and detection. A major objective in the design of this instrumentation is to develop the capacity to evaluate much larger areas of tissue. An approach to fulfilling this objective is the creation of a gasket that can seal individual wells of a nitrocellulose array. A gasket was created by laser cutting an assembly of molded silicone rubber and a double-sided tape (silicone-acrylic). Experiments showed when the gasket was adhered to a glass slide and subjected to the PCR, there was no leakage. FAST Slides, nitrocellulose slides provided by Grace Bio-Labs, are cut with a laser to generate the nitrocellulose arrays.
by Helena Nien-Hwa Chia.
S.B.
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Malan, Stefanie. "Real time PCR as a versatile tool for virus detection and transgenic plant analysis." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/1921.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2009.
ENGLISH ABSTRACT: South Africa is regarded as one of the top wine producing countries in the world. One of the threats to the sustainability of the wine industry is viral diseases of which Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine virus A (GVA) are considered to be the most important and wide spread. Scion material is regularly tested for viruses; however scion material is often grafted onto rootstocks that have questionable phytosanitary status. Virus detection in rootstocks is challenging due to low and varying titres, but is imperative as a viral control mechanism. An additional viral control mechanism is the use of transgenic grapevine material which offers resistance to grapevine infection. The objective of this project was to establish a detection system using real time PCR (qPCR) techniques, to accurately and routinely detect GLRaV-3 and GVA in rootstock propagation material. qPCR would furthermore be used to perform molecular characterisation of transgenic plants containing a GLRaV-3 antiviral ΔHSP-Mut construct. A severely infected vineyard (Nietvoorbij farm) in the Stellenbosch area was screened throughout the grapevine growing season to investigate virus prevalence throughout the season and to determine the optimal time for sensitive virus detection. A large scale screening of nursery propagation material for GLRaV-3 infection was also conducted. The qRT-PCR results were compared to DAS-ELISA results to compare the efficacy and sensitivity of the two techniques. For the severely infected vineyard, the ability to detect GLRaV-3 increased as the season progressed towards winter. qRT-PCR was more sensitive and accurate in detecting GLRaV-3 than DASELISA, as the latter technique delivered numerous false positive results later in the season. The best time to screen for GLRaV-3 in the Western Cape region was from the end of July to September. For the nursery screenings, our qRT-PCR results were compared to the results of the DAS-ELISA performed by the specific nurseries. No GLRaV-3 infection was detected in the specific samples received from the two different nurseries. The results for all the samples correlated between the two techniques. This confirms that the propagation material of these nurseries has a healthy phytosanitary status with regards to GLRaV-3. However, the detection of GVA in the severely infected vineyard yielded inconsistent results. Detection ability fluctuated throughout the season and no specific trend in seasonal variation and virus titre fluctuation could be established. The highest percentage of GVA infected samples were detected during September, April and the end of July. Previously published universal primers were used for the detection of GVA, but further investigation indicated that they might not be suitable for sensitive detection of specific GVA variants present in South Africa. Vitis vinifera was transformed with a GLRaV-3 antiviral construct, ΔHSP-Mut. SYBR Green Real time PCR (qPCR) and qRT-PCR were utilised as alternative methods for molecular characterisation of transgenic plants. The qPCR and Southern blot results correlated for 76.5% of the samples. This illustrated the ability of qPCR to accurately estimate transgene copy numbers. Various samples were identified during qRT-PCR amplification that exhibited high mRNA expression levels of the transgene. These samples are ideal for further viral resistance studies. This study illustrated that the versatility of real time PCR renders it a valuable tool for accurate virus detection as well as copy number determination.
AFRIKAANSE OPSOMMING: Suid Afrika word geag as een van die top wyn produserende lande ter wereld. Die volhoubaarheid van die wynbedryf word onder andere bedreig deur virus-infeksies. Grapevine leafroll associated virus 3 (GLRaV-3) en Grapevine virus A (GVA) is van die mees belangrike virusse wat siektes veroorsaak in Suid-Afrikaanse wingerde. Wingerd bo-stok materiaal word gereeld getoets vir hierdie virusse, maar hierdie materiaal word meestal geënt op onderstokmateriaal waarvan die virus status onbekend is. Virus opsporing in onderstokke word egter gekompliseer deur baie lae en variërende virus konsentrasies, maar opsporing in voortplantingsmateriaal is ‘n noodsaaklike beheermeganisme vir virus-infeksie. Die doel van die projek was om ‘n opsporingsisteem te ontwikkel via kwantitatiewe PCR (qPCR) tegnieke vir akkurate en gereelde toetsing van GLRaV-3 en GVA in onderstokmateriaal. qPCR sal ook verder gebruik word vir molekulêre karakterisering van transgeniese plante wat ‘n GLRaV-3 antivirale ΔHSP-Mut konstruk bevat. ‘n Hoogs geïnfekteerde wingerd was regdeur die seisoen getoets om seisoenale fluktuasies in viruskonsentrasie te ondersoek en om die optimale tydstip vir sensitiewe virus opsporing te bepaal. ‘n Grootskaalse toetsing van kwekery voortplantingsmateriaal vir GLRaV-3 infeksie was ook uitgevoer. Die qRT-PCR resultate is met die DAS-ELISA resultate vergelyk om die effektiwiteit en sensitiwiteit van die twee tegnieke te vergelyk. Vir die hoogs geïnfekteerde wingerd het die GLRaV-3 opsporing toegeneem met die verloop van die seisoen tot en met winter. qRT-PCR was meer sensitief en akkuraat as DAS-ELISA in die opsporing van GLRaV-3, weens verskeie vals positiewe resultate wat later in die seisoen deur die laasgenoemde tegniek verkry is. Die beste tyd om vir GLRaV-3 te toets is vanaf einde Julie tot September. Tydens die kwekery toetsings was qRT-PCR resultate met die DAS-ELISA resultate van die spesifieke kwekerye vergelyk. Geen GLRaV-3 infeksie was waargeneem in die spesifieke monsters wat vanaf die kwekerye ontvang is nie. Die resultate van die twee tegnieke het ooreengestem vir al die monsters wat v getoets is. Dit het bevestig dat die voortplantingsmateriaal van hierdie kwekerye gesonde fitosanitêre status met betrekking tot GLRaV-3 gehad het. Die opsporing van GVA in die geïnfekteerde wingerd het egter wisselvallige resultate gelewer. Opsporing van die virus het ook regdeur die seisoen gefluktueer en geen spesifieke neiging in seisoenale opsporingsvermoë kon gemaak word nie. Die hoogste persentasie GVA geïnfekteerde monsters was waargeneem tydens September, April en die einde van Julie. Voorheen gepubliseerde universele inleiers was gebruik vir die opsporing van GVA, maar verdere ondersoeke het getoon dat hierdie inleiers nie noodwendig geskik is vir sensitiewe opsporing van GVA variante wat teenwoordig is in Suid-Afrika nie. Vitis vinifera was getransformeer met ‘n GLRaV-3 antivirale konstruct, ΔHSP-Mut. SYBR Green Real time PCR (qPCR) en qRT-PCR was ingespan as alternatiewe metodes vir molekulêre karaterisering van transgeniese plante. Die qPCR en Southern-klad resultate het ooreengestem vir 76.5% van die monsters. Dit illustreer die vermoë van qPCR om akkurate kopie-getalle van transgene te bepaal. Verskeie plante is geïdentifiseer tydens qRT-PCR amplifisering wat hoë vlakke van transgeen mRNA uitdrukking getoon het. Hierdie monsters is ideaal vir verdere virus weerstandbiedendheids studies. Hierdie studie het die veelsydigheid van real time PCR bewys en getoon dat dit ‘n kosbare tegniek is vir akkurate virus opsporing sowel as kopie-getal bepaling.
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Amiri, Mehdi. "Real-time PCR detection and PFGE typing of Pseudomonas aeruginosa from cystic fibrosis patients." Doctoral thesis, Università Politecnica delle Marche, 2016. http://hdl.handle.net/11566/243098.

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Pseudomonas aeruginosa (PA) infection is the main cause of mortality in Cystic Fibrosis (CF) patients. Rapid and sensitive PA detection is pivotal to improve the prognosis. This thesis includes two parts. The first focuses on the development of a Real-time PCR protocol to detect dormant- non-culturable PA in sputum samples from CF patients (CFPA). 12 culture-positive (CP) and 29 culture-negative (CN) samples were analyzed by a qPCR targeting ecfX, 24% resulted positive (P), with bacterial counts (102 - 106 cells/ml) also higher than those obtained for CP samples. A significant association of culture-negative/PCR-positive samples from chronic patients with symptoms relapse (P=0.018) and following culture-positive samples (P=0.034) was found. These findings stress the value of qPCR approaches in monitoring P. aeruginosa colonization and foreseeing symptom recurrence in CF patients. In second part we investigated the epidemiological relatedness of CFPA. 77 CFPA and 19 PA from non-CF patients (NCFPA) were analysed by pulsed field gel electrophoresis (PFGE) profile, antibiotic resistance (AR) and mucous production. Population structure analysis showed an overall polyclonality, more evident among CF strains. 100% similarity was only found within each of 10 CFPA pairs from the same patient, and 95% between 2 CFPA isolates from different patients who never met each other in hospital, suggesting a common source of infection rather than cross-contamination. Among NCFPA four isolates from as many inpatients and two from as many outpatients showed the same pulsotype (H and M, respectively). AR was more common among CFPA and nonmucoid phenotype. In particular, a significant (P=0.016) association was found between gentamycin-resistance CFPA and nonmucoid phenotype. AR patterns were however highly variable, also among PA belonging to the same pulsotype; suggesting the lack of involvement of multiple antibiotic resistance (MAR) in cross-colonization.
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Books on the topic "Detection PCR real-time"

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Wolk, Donna M. Molecular methods for microsporidia detection: Use of an inhibitor control with real-time PCR. Denver, Colo: Awwa Research Foundation, 2007.

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Soto Varela, Zamira, David Rosado Porto, Jairo Ceballos Sandoval, José Villarreal Camacho, Hernando Bolívar Anillo, Christian Orozco Sánchez, Camila Pichón González, Dalidier Estrada Alvarado, Bertha Granados Pantoja, and María Badillo Viloria. Real-time PCR applied to bacterial waterborne pathogens detection and quantification. Edited by María Badillo Viloria and Liliana Pérez Lavalle. Translated by David Rosado Porto. Ediciones Universidad Simón Bolívar, 2017. http://dx.doi.org/10.17081/bonga.2616.

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Weiping, Chu, Choi Sam, and Dezfulian H. Molecular Alternatives to Indicator and Pathogen Detection: Real-Time PCR (Werf Report). WERF, 2006.

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Fernando, Jose Heavena, and Jose Heavena. HIV: Molecular Detection and Analysis of HIV-1 Proviral DNA Using Real Time-PCR and Flow Cytometry. Independently Published, 2019.

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Single-species detection. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0009.

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Chapter 9 “Single-species detection” deals with the practical aspects of detecting a single and predefined taxon with eDNA, with a particular focus on the use of quantitative PCR (qPCR) for this purpose. After presenting how single-species detection has been implemented in a few seminal studies, it details the principles underlying qPCR. More specifically, it describes the typical qPCR amplification curve and the different systems (SYBR green and TaqMan probe assays) available to record amplicon accumulation in real time via fluorescence measurements. Chapter 9 also explains how the initial number of target sequences can be estimated with the Ct method, and addresses the design and test of reliable qPCR barcodes and probes targeting a single species. Finally, several important experimental considerations are highlighted, including the particular concerns of contamination and inhibition in qPCR.
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Dyer, Paul S., Carol A. Munro, and Rosie E. Bradshaw. Fungal genetics. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0005.

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Fungi have been long used as model organisms to investigate genetic and cellular processes. An overview is provided of how fungi function at a genetic level, including ploidy, gene structure, and gene flow by sexual and asexual processes. The tools used to study fungal genetics are then described, such techniques having widespread applications in medical mycology research. Classical genetic analysis includes the use of gene mapping by sexual crossing and tetrad analysis, and forward genetic experimentation based on mutagenesis, for which various mutant screening approaches are described. Molecular genetic analysis includes gene manipulation by transformation; different methods for gene knockout and targeting, and their application for forward and reverse genetic approaches, are outlined. Finally, molecular genetic methods used to study gene expression and function are reviewed, including use of inducible or constitutive overexpression, real-time PCR, cellular localization of gene products by fluorescent tagging, and detection of protein–protein interactions.
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Book chapters on the topic "Detection PCR real-time"

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Barletta, Francesca, Theresa J. Ochoa, and Thomas G. Cleary. "Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic." In PCR Detection of Microbial Pathogens, 307–14. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-60327-353-4_21.

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Hedman, Johannes, and Peter Rådström. "Overcoming Inhibition in Real-Time Diagnostic PCR." In PCR Detection of Microbial Pathogens, 17–48. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-60327-353-4_2.

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Wang, Zhiguo, and Baofeng Yang. "Single Cell Stem-Looped Real-Time PCR." In MicroRNA Expression Detection Methods, 361–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-04928-6_31.

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Bernard, Philip S., Astrid Reiser, and Gregory H. Pritham. "Mutation Detection by Fluorescent Hybridization Probe Melting Curves." In Rapid Cycle Real-Time PCR, 11–19. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_2.

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Johnson, Gemma, Tania Nolan, and Stephen A. Bustin. "Real-Time Quantitative PCR, Pathogen Detection and MIQE." In PCR Detection of Microbial Pathogens, 1–16. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-60327-353-4_1.

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Winchell, Jonas M., and Stephanie L. Mitchell. "Detection of Mycoplasma pneumoniae by Real-Time PCR." In PCR Detection of Microbial Pathogens, 149–58. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-60327-353-4_10.

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Kleinle, Stephanie, and Sabina Gallati. "Detection of the Mitochondrial DNA Mutation MELAS3243 Using Hybridization Probes." In Rapid Cycle Real-Time PCR, 153–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_18.

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Reischl, Udo, Birgit Leppmeier, Markus Heep, Daniela Beck, and Norbert Lehn. "Rapid and Specific Detection of Helicobacter pylori by LightCycler PCR." In Rapid Cycle Real-Time PCR, 323–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_34.

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Kessler, Harald H. "Qualitative Detection of Herpes Simplex Virus DNA on the LightCycler." In Rapid Cycle Real-Time PCR, 331–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_35.

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van den Berg, Renate Johanna, Dennis Bakker, and Ed J. Kuijper. "Diagnosis of Clostridium difficile Infection Using Real-Time PCR." In PCR Detection of Microbial Pathogens, 247–56. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-60327-353-4_16.

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Conference papers on the topic "Detection PCR real-time"

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Woudenberg, Timothy M., and J. Stevens. "Quantitative PCR by real-time detection." In Photonics West '96, edited by Gerald E. Cohn, Steven A. Soper, and C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237619.

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BARLOCCHI, G., U. MASTROMATTEO, S. SASSOLINI, M. SCURATI, and F. VILLA. "MICROFLUIDIC DEVICE FOR REAL TIME PCR DETECTION." In Proceedings of the 9th Italian Conference. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812701770_0058.

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Kim, Sangheon, Sunjak Choi, Yeji Lee, Sangyoon Jung, Minhyoung Jung, Jieun Baek, Hyeonuk Sim, Donghyun Kang, and Junho Lee. "Optical System for Multi-Channel Real-Time PCR Detection." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2018. http://dx.doi.org/10.1364/cleo_at.2018.jtu2a.96.

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Schmid, Silke, and J. Bauer. "Detection of salmonellae by real-time PCR within 8 hours." In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/safepork-180809-1191.

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Ma, Yutong, Liang Zeng, and Jianhuan Zhang. "A fluorescence detection optical system for real-time quantitative PCR." In Optical Design and Testing X, edited by Rengmao Wu, Osamu Matoba, Yongtian Wang, and Tina E. Kidger. SPIE, 2020. http://dx.doi.org/10.1117/12.2574901.

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Croci, L., D. de Medici, S. di Pasquale, E. Delibato, and L. Toti. "SYBR Green Real-Time PCR for Salmonella detection in meat products." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-539.

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"Detection of Gene Expression from Vitis by Real Time Quantitative RT-PCR." In 2018 4th World Conference on Control, Electronics and Computer Engineering. Francis Academic Press, 2018. http://dx.doi.org/10.25236/wccece.2018.15.

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Manrique, Javier, Gustavo Sarria, Abelardo Arias, Pamela Mora, Abel Limache, Maria del Carmen Nuñez, Yasser Sullcahuaman, and Tatiana Vidaurre. "Abstract 3488: HPV detection by PCR in real time in peruvian women." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3488.

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Chen, Wilfred, Grisselle Martinez, and Ashok Mulchandani. "Detection of salmonella using a real-time PCR based on molecular beacons." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari, and Weihong Tan. SPIE, 2000. http://dx.doi.org/10.1117/12.380515.

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Houde, Alain, D. Leblanc, P. Ward, E. Poitras, M. J. Gagné, Ann Letellier, J. Brassard, and D. Plante. "Detection of Hepatitis E virus in swine using real-time RT-PCR." In Eighth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-830.

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Reports on the topic "Detection PCR real-time"

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McAvin, James C., and Carl J. Mason. Norovirus Real Time RT-PCR Detection Technology Transition to the Joint Biological Identification and Diagnosis System (JBAIDS). Fort Belvoir, VA: Defense Technical Information Center, September 2012. http://dx.doi.org/10.21236/ada568257.

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Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.

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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA array to study the expression of KHV genes in different conditions. Background to the topics-Mass mortality of koi or common carp has been observed in Israel, USA, Europe and Asia. These outbreaks have reduced exports of koi from Israel and have created fear about production, import, and movements of koi and have raised concerns about potential impacts on native cyprinid populations in the U.S.A. Major conclusions-A suite of new diagnostic tools was developed that included 3 PCR assays for detection of KHV DNA in cell culture and fish tissues and an ELISA assay capable of detecting anti-KHV antibodies in the serum of koi and common carp. The TKPCR assay developed during the grant has become an internationally accepted gold standard for detection of viral DNA. Additionally, the ELISA developed for detecting serum anti-KHV antibodies is now in wide use as a major nonlethal screening tool for evaluating virus status of koi and common carp populations. Real time PCR assays have been able to detect viral DNA in the internal organs of survivors of natural and wild type vaccine exposures at 1 and 10³ genome equivalents at 7 months after exposure. In addition, vaccinated fish were able to transmit the virus to naive fish. Potential control utilizing hybrids of goldfish and common carp for production demonstrated they were considerably more resistant than pure common carp or koi to both KHV (CyHV-3). There was no evidence that goldfish or other tested endemic cyprinids species were susceptible to KHV. The complete genomic sequencing of 3 strains from Japan, the USA, and Israel revealed a 295 kbp genome containing a 22 kbp terminal direct repeat encoding clear gene homologs to other fish herpesviruses in the family Herpesviridae. The genome encodes156 unique protein-coding genes, eight of which are duplicated in the terminal repeat. Four to seven genes are fragmented and the loss of these genes may be associated with the high virulence of the virus. Viral gene expression was studies by a newly developed chip which has allowed verification of transcription of most all hypothetical genes (ORFs) as well as their kinetics. Implications, both scientific and agricultural- The results from this study have immediate application for the control and management of KHV. The proposal provides elements key to disease management with improved diagnostic tools. Studies on the ecology of the virus also provide insights into management of the virus at the farms that farmers will be able to apply immediately to reduce risks of infections. Lastly, critical issues that surround present procedures used to create “resistant fish” must be be resolved (e.g. carriers, risks, etc.). Currently stamping out may be effective in eradicating the disease. The emerging disease caused by KHV continues to spread. With the economic importance of koi and carp and the vast international movements of koi for the hobby, this disease has the potential for even further spread. The results from our studies form a critical component of a comprehensive program to curtail this emerging pathogen at the local, regional and international levels.
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