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Journal articles on the topic 'DFRT'

Author: Grafiati

Published: 29 March 2025

Last updated: 31 July 2025

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1

Caspers, Patrick, Luc Bury, Bérangère Gaucher, et al. "In Vitro and In Vivo Properties of Dihydrophthalazine Antifolates, a Novel Family of Antibacterial Drugs." Antimicrobial Agents and Chemotherapy 53, no. 9 (2009): 3620–27. http://dx.doi.org/10.1128/aac.00377-09.

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ABSTRACT Racemic 2,4-diaminopyrimidine dihydrophthalazine derivatives BAL0030543, BAL0030544, and BAL0030545 exhibited low in vitro MICs toward small, selected panels of Enterococcus faecalis, Enterococcus faecium, Streptococcus pneumoniae, Moraxella catarrhalis, and Mycobacterium avium, though the compounds were less active against Haemophilus influenzae. The constellation of dihydrofolate reductases (DHFRs) present in 20 enterococci and 40 staphylococci was analyzed and correlated with the antibacterial activities of the dihydrophthalazines and trimethoprim. DHFRs encoded by dfrB, dfrA (S1 i

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Hamid, Aabid, Atul Anand, and Ram Kinkar Roy. "The charge transfer limit of a chemical adduct: the role of perturbation on external potential." Physical Chemistry Chemical Physics 19, no. 17 (2017): 10905–12. http://dx.doi.org/10.1039/c7cp01208j.

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Liu, Li, and Ji Chun Tan. "Multiple Watermarks Embedding Simultaneously to Identify Image Status Based on Fractional Fourier Transform." Advanced Materials Research 341-342 (September 2011): 758–62. http://dx.doi.org/10.4028/www.scientific.net/amr.341-342.758.

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A new method of multiple watermarks embedding simultaneously to identify image status based on once and twice discrete fractional Fourier transform (DFRT) is presented. Random series watermark, chirp watermark and annotation watermark are embedded simultaneously in fractional Fourier transform domain to identify digital tamper detection, scanning detection, duplicate detection. According to the extracting situation of three kinds of watermarks from an image and the twice DFRT amplitude spectrum, we can know whether the image is tampered, scanned or duplicated. It provides a convenient way to i

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Saha, Bapan, and Pradip Kr Bhattacharyya. "Understanding reactivity, aromaticity and absorption spectra of carbon cluster mimic to graphene: a DFT study." RSC Advances 6, no. 83 (2016): 79768–80. http://dx.doi.org/10.1039/c6ra15016k.

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Effect of doping B and/or N on the reactivity, aromaticity and absorption spectra of graphene and functionalized (–OH and –COOH) carbon cluster mimicking graphene is studied using DFT, DFRT and TD-DFT.

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Jugal, Charan Sarmah, Sinha Sourab, Sarmah Nabajit, Kumar Purkayastha Siddhartha, Sarkar Priyanka, and Kr. Bhattacharyya Pradip. "DFRT studies of amide-coupled benzoic nitrogen mustard derivatives." Journal of Indian Chemical Society Vol. 91, Dec 2014 (2014): 2211–16. https://doi.org/10.5281/zenodo.5744955.

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Department of Chemistry, Arya Vidyapeeth College, Guwahati-781 016, Assam, India <em>E-mail :</em> prdpbhatta@yahoo.com <em>Manuscript received online 19 May 2014, revised 04 July 2014, accepted 04 July 2014</em> Reactivity of aziridinium ion as well as mono-adducts formed during alkylation of DNA (with guanine) by few amide-coupled benzoic nitrogen mustard derivatives are analysed using density functional reactivity theory (DFRT). Global and local reactivity descriptors are used to compare the reactivity of the aziridinium ion intermediates and monoadducts. Our results clearly explain the rea

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Shishido, E., S. Higashijima, Y. Emori, and K. Saigo. "Two FGF-receptor homologues of Drosophila: one is expressed in mesodermal primordium in early embryos." Development 117, no. 2 (1993): 751–61. http://dx.doi.org/10.1242/dev.117.2.751.

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The fibroblast growth factor (FGF)/receptor system is thought to mediate various developmental events in vertebrates. We examined molecular structures and expression of DFR1 and DFR2, two Drosophila genes closely related to vertebrate FGF-receptor genes. DFR1 and DFR2 proteins contain two and five immunoglobulin-like domains, respectively, in the extracellular region, and a split tyrosine kinase domain in the intracellular region. In early embryos, DFR1 RNA expression, requiring both twist and snail proteins, is specific to mesodermal primordium and invaginated mesodermal cells. At later stage

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Choudhary, Nancy, and Boas Pucker. "Conserved amino acid residues and gene expression patterns associated with the substrate preferences of the competing enzymes FLS and DFR." PLOS ONE 19, no. 8 (2024): e0305837. http://dx.doi.org/10.1371/journal.pone.0305837.

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Background Flavonoids, an important class of specialized metabolites, are synthesized from phenylalanine and present in almost all plant species. Different branches of flavonoid biosynthesis lead to products like flavones, flavonols, anthocyanins, and proanthocyanidins. Dihydroflavonols form the branching point towards the production of non-colored flavonols via flavonol synthase (FLS) and colored anthocyanins via dihydroflavonol 4-reductase (DFR). Despite the wealth of publicly accessible data, there remains a gap in understanding the mechanisms that mitigate competition between FLS and DFR f

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Gibreel, Amera, and Ola Sköld. "High-Level Resistance to Trimethoprim in Clinical Isolates of Campylobacter jejuni by Acquisition of Foreign Genes (dfr1 and dfr9) Expressing Drug-Insensitive Dihydrofolate Reductases." Antimicrobial Agents and Chemotherapy 42, no. 12 (1998): 3059–64. http://dx.doi.org/10.1128/aac.42.12.3059.

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ABSTRACT The pathogenic bacterium Campylobacter jejuni has been regarded as endogenously resistant to trimethoprim. The genetic basis of this resistance was characterized in two collections of clinical isolates of C. jejuni obtained from two different parts of Sweden. The majority of these isolates were found to carry foreigndfr genes coding for resistant variants of the dihydrofolate reductase enzyme, the target of trimethoprim. The resistance genes, found on the chromosome, were dfr1 anddfr9. In about 10% of the strains, the dfr1and dfr9 genes occurred simultaneously. About 10% of the examin

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Hamza, Rafik, Alzubair Hassan, Teng Huang, Lishan Ke, and Hongyang Yan. "An Efficient Cryptosystem for Video Surveillance in the Internet of Things Environment." Complexity 2019 (December 9, 2019): 1–11. http://dx.doi.org/10.1155/2019/1625678.

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Surveillance systems paradigm envisions the pervasive interconnection and cooperation of interactive devices over the Internet infrastructure. Nevertheless, dissemination and processing of surveillance video amid the Internet of Things (IoT) applications become a susceptible issue due to the large volume and the significant information of these data. Moreover, surveillance devices on IoT have very limited resources such as memory and storage. The actual security methods are not quite appropriate for surveillance IoT systems. Thus, a particular cryptosystem technique is required for surveillanc

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Liu, Xifeng, Wen Yuan, Chaokang Gu, Wenhao Huang, and Weixin Hu. "Nature of Sigma-Type Lithium Bonding Interaction in Nanoscale." Nano LIFE 04, no. 04 (2014): 1441020. http://dx.doi.org/10.1142/s1793984414410207.

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Li-based rechargeable batteries are becoming popular power sources for biomedical devices and healthcare equipment. In nanoscale, lithium bonds may associate with intermolecular noncovalent interactions when Li atoms are shared by adjacent atoms inside rechargeable Li batteries. Theoretical study of lithium boding interactions thus is of paramount importance for a better understanding of the working mechanisms and designing high-efficient electrode–electrolyte interfaces from nanolevel for Li-based batteries. In this study, we used state-of-the-art theoretical methods, with inclusion of densit

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Bergmann, René, Mark van der Linden, Gursharan S. Chhatwal, and D. Patric Nitsche-Schmitz. "Factors That Cause Trimethoprim Resistance in Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 58, no. 4 (2014): 2281–88. http://dx.doi.org/10.1128/aac.02282-13.

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ABSTRACTThe use of trimethoprim in treatment ofStreptococcus pyogenesinfections has long been discouraged because it has been widely believed that this pathogen is resistant to this antibiotic. To gain more insight into the extent and molecular basis of trimethoprim resistance inS. pyogenes, we tested isolates from India and Germany and sought the factors that conferred the resistance. Resistant isolates were identified in tests for trimethoprim or trimethoprim-sulfamethoxazole (SXT) susceptibility. Resistant isolates were screened for the known horizontally transferable trimethoprim-insensiti

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ZHAO, DONGBO, CHUNYING RONG, DULIN YIN, and SHUBIN LIU. "MOLECULAR ACIDITY OF BUILDING BLOCKS OF BIOLOGICAL SYSTEMS: A DENSITY FUNCTIONAL REACTIVITY THEORY STUDY." Journal of Theoretical and Computational Chemistry 12, no. 05 (2013): 1350034. http://dx.doi.org/10.1142/s021963361350034x.

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An accurate prediction of the molecular acidity by employing ab initio or density functional approaches for typical molecular systems is still challenging. Recently, we proposed to utilize two quantum descriptors, molecular electrostatic potential (MEP) and the sum of valence natural atomic orbital (NAO) energies on the nucleus of both the acidic atom and leaving proton, to quantitatively evaluate the pKa values. This new approach has been validated by a number of organic and inorganic systems and justified within the framework of density functional reactivity theory (DFRT). In this work, we a

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Oz, Alon, Shany Hershkovitz, Nataly Belman, Ervin Tal-Gutelmacher, and Yoed Tsur. "Analysis of impedance spectroscopy of aqueous supercapacitors by evolutionary programming: Finding DFRT from complex capacitance." Solid State Ionics 288 (May 2016): 311–14. http://dx.doi.org/10.1016/j.ssi.2015.11.008.

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Boukamp, Bernard A., and Aurélie Rolle. "Use of a distribution function of relaxation times (DFRT) in impedance analysis of SOFC electrodes." Solid State Ionics 314 (January 2018): 103–11. http://dx.doi.org/10.1016/j.ssi.2017.11.021.

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Bar, Nimai, and Rajkamal Pandit. "IMPEDANCE SPECTROSCOPY GENETIC PROGRAMMING (ISGP) ANALYSES SETTING PROPER WORKING CONDITIONS OF A POLYMER ELECTROLYTE MEMBRANE FUEL CELL." Journal of Advanced Scientific Research 14, no. 07 (2023): 01–07. http://dx.doi.org/10.55218/jasr.202314701.

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   This study demonstrates application of Impedance Spectroscopy Genetic Program (ISGP) for the investigation of a polymer electrolyte membrane fuel cell (PEMFC). It further demonstrates the procedures to optimize the operating conditions of a single cell in a test station. To do that, the effects of temperature, hydrogen/air, and dew point temperature (DPT) on the cell were examined using an Arbin test station. ISGP followed a two-iteration procedure. First, find an out-of-range peak (at high frequencies) that corresponds to the ohmic (series) resistance of the system. Secon

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Melo, B. M. G., Francisco J. A. Loureiro, Duncan P. Fagg, L. C. Costa, and M. P. F. Graça. "DFRTtoEIS: An easy approach to verify the consistency of a DFRT generated from an impedance spectrum." Electrochimica Acta 366 (January 2021): 137429. http://dx.doi.org/10.1016/j.electacta.2020.137429.

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Belkić, Dževad, and Karen Belkić. "In vivo derivative NMR spectroscopy for simultaneous improvements of resolution and signal-to-noise-ratio: Case study, Glioma." Journal of Mathematical Chemistry 59, no. 9 (2021): 2133–78. http://dx.doi.org/10.1007/s10910-021-01280-0.

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AbstractThe theme of this study is derivative nuclear magnetic resonance (dNMR) spectroscopy. This versatile methodology of peering into the molecular structure of general matter is common to e.g. analytical chemistry and medical diagnostics. Theoretically, the potential of dNMR is huge and the art is putting it into practice. The implementation of dNMR (be it in vitro or in vivo) is wholly dependent on the manner in which the encoded time signals are analyzed. These acquired data contain the entire information which is, however, opaque in the original time domain. Their frequency-dependent du

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Sarmah, Amrit, and Ram Kinkar Roy. "A density functional reactivity theory (DFRT) based approach to understand the interaction of cisplatin analogues with protecting agents." Journal of Computer-Aided Molecular Design 28, no. 12 (2014): 1153–73. http://dx.doi.org/10.1007/s10822-014-9790-7.

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Adesoji, Tomiwa Olumide, Beverly Egyir, and Adebayo Osagie Shittu. "Antibiotic-resistant staphylococci from the wastewater treatment plant and grey-water samples in Obafemi Awolowo University, Ile-Ife, Nigeria." Journal of Water and Health 18, no. 6 (2020): 890–98. http://dx.doi.org/10.2166/wh.2020.019.

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Abstract This study examined the occurrence and molecular basis for antibiotic-resistant staphylococci from the wastewater treatment plant and grey-water samples in Obafemi Awolowo University, Nigeria. Standard microbiological techniques and molecular methods were utilized. The species identified (MALDI score &gt;1.7) comprised S. saprophyticus (19), S. cohnii (8), S. sciuri (7), S. aureus (4), S. epidermidis (3), S. warneri (2), S. equorum (1), S. haemolyticus (1), S. nepalensis (1), S. condimenti (1), and S. pasteuri (1). Resistance to trimethoprim, tetracycline and cefoxitin were observ

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Bardasheva, Alevtina, Artem Tikunov, Yuliya Kozlova, et al. "Antibiotic Resistance and Pathogenomics of Staphylococci Circulating in Novosibirsk, Russia." Microorganisms 9, no. 12 (2021): 2487. http://dx.doi.org/10.3390/microorganisms9122487.

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A total of 394 strains of staphylococci found in humans and pets in Novosibirsk, Siberian Russia, were characterized in terms of antibiotic resistance and corresponding genes. Two coagulase-positive and 17 coagulase-negative species were identified. The majority of isolates, with the exception of S. haemolyticus and hospital S. epidermidis isolates, were sensitive to most of the tested antibiotics, and isolates from pets displayed the lowest level of resistance. Nevertheless, methicillin-resistant (MRS) and/or multidrug-resistant (MDR) isolates were found in all prevailed species, including co

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Ishioka, Shigeki, Takashi Hosokawa, Taro Ikeda, et al. "Therapeutic potential of mature adipocyte-derived dedifferentiated fat cells for inflammatory bowel disease." Pediatric Surgery International 36, no. 7 (2020): 799–807. http://dx.doi.org/10.1007/s00383-020-04681-5.

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Abstract Purpose Our previous studies demonstrated that mature adipocyte-derived dedifferentiated fat (DFAT) cells possess similar multipotency as mesenchymal stem cells. Here, we examined the immunoregulatory potential of DFAT cells in vitro and the therapeutic effect of DFAT cell transplantation in a mouse inflammatory bowel disease (IBD) model. Methods The effect of DFAT cell co-culture on T cell proliferation and expression of immunosuppression-related genes in DFAT cells were evaluated. To create IBD, CD4+CD45RBhigh T cells were intraperitoneally injected into SCID mice. One week later, D

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Shishido, E., N. Ono, T. Kojima, and K. Saigo. "Requirements of DFR1/Heartless, a mesoderm-specific Drosophila FGF-receptor, for the formation of heart, visceral and somatic muscles, and ensheathing of longitudinal axon tracts in CNS." Development 124, no. 11 (1997): 2119–28. http://dx.doi.org/10.1242/dev.124.11.2119.

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DFR1 encodes a mesoderm-specific fibroblast growth factor receptor in Drosophila. Here, we identified and characterized a protein-null mutant of DFR1 and examined DFR1 expression in embryos using anti-DFR1 antibody. Mutant phenotypes were completely rescued by a genomic fragment from the DFR1 locus. After invagination, mesodermal cells expressing DFR1 undergo proliferation and spread out dorsally to form a monolayer beneath the ectoderm. In mutant embryos, however, the mesoderm is not capable of extending to the normal dorsal limit and consequently mesodermal cells fail to receive ectodermal s

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Goriachuk, V. F., and D. F. Dukov. "INSTITUTIONAL PROVISION INVESTMENT INSTRUMENTS OF PUBLIC ADMINISTRATION REGIONAL DEVELOPMENT." Economic innovations 19, no. 3(65) (2017): 47–55. http://dx.doi.org/10.31520/ei.2017.19.3(65).47-55.

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During the years of independence in Ukraine, a certain set of investment instruments of state governance for regional development has been created: state and regional target programs, regional development agreements, agreements on implementation of interregional projects, programs for overcoming the state of depression, the State Fund for Regional Development (DFRD), and others. However, their level of performance is quite low.Notwithstanding the provisions of the State Strategy for Regional Development for the period up to 2020, regional development agreements and programs to overcome the sta

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Coque, Teresa M., Kavindra V. Singh, George M. Weinstock, and Barbara E. Murray. "Characterization of Dihydrofolate Reductase Genes from Trimethoprim-Susceptible and Trimethoprim-Resistant Strains ofEnterococcus faecalis." Antimicrobial Agents and Chemotherapy 43, no. 1 (1999): 141–47. http://dx.doi.org/10.1128/aac.43.1.141.

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Enterococci are usually susceptible in vitro to trimethoprim; however, high-level resistance (HLR) (MICs, >1,024 μg/ml) has been reported. We studied Enterococcus faecalis DEL, for which the trimethoprim MIC was >1,024 μg/ml. No transfer of resistance was achieved by broth or filter matings. Two different genes that conferred trimethoprim resistance when they were cloned in Escherichia coli (MICs, 128 and >1,024 μg/ml) were studied. One gene that coded for a polypeptide of 165 amino acids (MIC, 128 μg/ml forE. coli) was identical to dfr homologs that we cloned from a trimethoprim-susc

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Wang, Huan, Pinghua Li, Taoran Du, et al. "Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs." Animals 9, no. 12 (2019): 1039. http://dx.doi.org/10.3390/ani9121039.

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The aims of this study were to assess the effects of increasing levels of DFRB as a replacement for corns on intestinal physical barrier function and bacteria of finishing pigs. A total of 35 castrated finishing pigs (age: 158.5 ± 2.0 d, initial body weight: 62.9 ± 0.8 kg) were randomly divided into five dietary treatments (seven replicates/treatment) for a 28-day experimental period, i.e., a control diet with basal diet, and four experimental diets in which maize was replaced by 7%, 14%, 21%, and 28% DFRB, respectively. The results showed that serum endotoxins concentration and diamine oxidas

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Cellier-Goetghebeur, Stella, Kiana Lafontaine, Claudèle Lemay-St-Denis, et al. "Discovery of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases in Diverse Environmental Settings Suggests an Evolutionary Advantage Unrelated to Antibiotic Resistance." Antibiotics 11, no. 12 (2022): 1768. http://dx.doi.org/10.3390/antibiotics11121768.

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Type B dihydrofolate reductases (DfrB) are intrinsically highly resistant to the widely used antibiotic trimethoprim, posing a threat to global public health. The ten known DfrB family members have been strongly associated with genetic material related to the application of antibiotics. Several dfrB genes were associated with multidrug resistance contexts and mobile genetic elements, integrated both in chromosomes and plasmids. However, little is known regarding their presence in other environments. Here, we investigated the presence of dfrB beyond the traditional areas of enquiry by conductin

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Akita, Daisuke, Naoki Tsukimura, Tomohiko Kazama, et al. "Regeneration of Two-Walled Infrabony Periodontal Defects in Swine After Buccal Fat Pad-Derived Dedifferentiated Fat Cell Autologous Transplantation." Biomolecules 15, no. 4 (2025): 604. https://doi.org/10.3390/biom15040604.

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Mature adipocyte-derived dedifferentiated fat (DFAT) cells show proliferative capabilities and multipotency. Given that the buccal fat pad (BFP) serves as a readily available resource for DFAT cell isolation, BFP-derived DFAT (BFP-DFAT) cells are a promising candidate in orofacial tissue engineering. In this research, we assessed the regenerative capacity of the periodontium through autologous BFP-DFAT cell transplantation in adult swine (micro-minipigs; MMPs). The BFP-DFAT cells were transplanted into inflammation-inducing two-walled infrabony periodontal defects located on the mesial of the

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Paul, Tanmoy, Nimrod Yavo, Igor Lubomirsky, and Yoed Tsur. "Determination of grain boundary conductivity using distribution function of relaxation times (DFRT) analysis at room temperature in 10 mol% Gd doped ceria: A non-classical electrostrictor." Solid State Ionics 331 (March 2019): 18–21. http://dx.doi.org/10.1016/j.ssi.2018.12.013.

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Sarmah, Amrit, and Ram Kinkar Roy. "A density functional reactivity theory (DFRT) based approach to understand the effect of symmetry of fullerenes on the kinetic, thermodynamic and structural aspects of carbon NanoBuds." Chemical Physics 472 (June 2016): 218–28. http://dx.doi.org/10.1016/j.chemphys.2016.03.006.

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Takabatake, Kiyofumi, Masakazu Matsubara, Eiki Yamachika, et al. "Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method." International Journal of Molecular Sciences 22, no. 22 (2021): 12392. http://dx.doi.org/10.3390/ijms222212392.

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Background: We investigated and compared the osteogenic potential and bone regeneration capacities of dedifferentiated fat cells (DFAT cells) and adipose-derived stem cells (ASCs). Method: We isolated DFAT cells and ASCs from GFP mice. DFAT cells were established by a new culture method using a mesh culture instead of a ceiling culture. The isolated DFAT cells and ASCs were incubated in osteogenic medium, then alizarin red staining, alkaline phosphatase (ALP) assays, and RT-PCR (for RUNX2, osteopontin, DLX5, osterix, and osteocalcin) were performed to evaluate the osteoblastic differentiation

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Chen, Xiaoshuai, Haiming Yang, and Zhiyue Wang. "The Effect of Different Dietary Levels of Defatted Rice Bran on Growth Performance, Slaughter Performance, Serum Biochemical Parameters, and Relative Weights of the Viscera in Geese." Animals 9, no. 12 (2019): 1040. http://dx.doi.org/10.3390/ani9121040.

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This study investigated the effect of different dietary levels of defatted rice bran (DFRB) on growth performance, slaughter performance, and relative weights of the viscera in geese. A total of 300 28-day-old healthy male Yangzhou goslings with similar body weights were randomly divided into five groups, with six replicates per group and 10 geese per replicate. The geese were fed diets containing 0%, 10%, 20%, 30%, or 40% DFRB for 42 days. Over the 29-d to 70-d trial period, no significant difference was observed in the average daily feed intake in geese (p > 0.05). However, 30% and 40% DF

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Zhao, Yinan, Zhongqing Zhao, Fangqiu Tong, Yu Fan, and Xiang Feng. "Modulus Waveform Design Based on Manifold ADMM Idea in Dual-Function Radar–Communication System." Electronics 13, no. 14 (2024): 2726. http://dx.doi.org/10.3390/electronics13142726.

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In this paper, we try to design the joint waveform and passive beamforming within the context of dual-function radar–communication (DFRC) systems. Focusing on the intricate trade-off between stringent radar beampattern constraints and their desired performance, we introduce a novel manifold idea based on the alternating direction method of multipliers (ADMM) framework. Specifically, our proposed method, named DFRC-MA, could address the challenge of constant modulus waveform design in a multiple-input–multiple-output (MIMO) DFRC system. Firstly, our methodology begins by formulating the referen

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Huang, Bingbing, Huangwei Shi, Li Wang, et al. "Effects of Defatted Rice Bran Inclusion Level on Nutrient Digestibility and Growth Performance of Different Body Weight Pigs." Animals 11, no. 5 (2021): 1374. http://dx.doi.org/10.3390/ani11051374.

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This study was conducted to determine the effects of low-protein diet prepared with different levels of defatted rice bran (DFRB) and weight stages on growth performance and nutrient digestibility of growing–finishing pigs. The animal experiment included three stages. A total of 240 growing pigs with an initial body weight of 28.06 ± 8.56 kg for stage 1 were allocated to five diets including one control group and four DFRB diets supplemented with 2.5%, 5%, 7.5% and 10% DFRB, respectively. The 192 crossbred pigs with initial body weights of 55.03 ± 7.31 kg and 74.55 ± 9.10 kg were selected for

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Tansriratanawong, Kallapat, Isao Tabei, Hiroshi Ishikawa, Akihiro Ohyama, Junko Toyomura, and Soh Sato. "Characterization and comparative DNA methylation profiling of four adipogenic genes in adipose-derived stem cells and dedifferentiated fat cells from aging subjects." Human Cell 33, no. 4 (2020): 974–89. http://dx.doi.org/10.1007/s13577-020-00379-x.

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Abstract Adipose-derived stem cells (ASCs) and dedifferentiated fat (DFAT) cells are alternative cell sources in tissue engineering and regeneration because they are easily obtained and exhibit multilineage differentiation. However, aging may attenuate their regenerative potential and metabolic functions. Reports characterizing DFAT cells derived from aging donors are rare, and comparisons of DNA methylation profiles between aging ASCs and DFAT cells are poorly understood. Therefore, this study aimed to characterize DFAT cells relative to ASCs derived from aging subjects and compare the DNA me

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Peng, Xuewu, Tongxing Song, Xiaoming Hu, et al. "Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term CultureIn Vitro." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/673651.

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It has been proved that terminally differentiated mature adipocytes possess abilities to dedifferentiate into fibroblast-like progeny cells with self-renewal and multiple differentiation, termed dedifferentiated fat (DFAT) cells. However, the biological properties of DFAT cells during long-term culturein vitrohave not been elucidated. Here, we obtained fibroblast-like morphology of porcine DFAT cells by ceiling culture. During the dedifferentiation process, round mature adipocytes with single large lipid droplets changed into spindle-shaped cells accompanied by the adipogenic markersPPARγ,aP2,

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Wang, Lian Rong, Naoki Ishiguro, Eiji Yamada, Yoshihiro Nishida, Koji Sato, and Hisashi Iwata. "The Effect of Da-Fang-Feng-Tang on Treatment of Type II Collagen-induced Arthritis in DBA/1 Mice." American Journal of Chinese Medicine 27, no. 02 (1999): 205–15. http://dx.doi.org/10.1142/s0192415x99000240.

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Da-Fang-Feng-Tang (DFFr), which is believed to be effective for treating human rheumatoid arthritis (RA), was given to DBA/1 mice at the onset of type II collagen-induced arthritis (CIA) to examine its effect. Granules of the crude DFFT extract were administered by gastric gavage at a dose of 1.6 g/kg/day for 12 weeks, starting the day CIA began. The levels of anticollagen IgG antibody were significantly decreased in the sera of the DFFT-treated group compared with the control group from weeks 2 to 7 after the onset of CIA. The severity of arthritis in the DFFT-treated group was markedly allev

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Huang, Bingbing, Li Wang, Zhiqian Lyu, et al. "Evaluation on Net Energy of Defatted Rice Bran from Different Origins and Processing Technologies Fed to Growing Pigs." Animals 11, no. 4 (2021): 1106. http://dx.doi.org/10.3390/ani11041106.

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The study was conducted to determine and compare the net energy (NE) of defatted rice bran (DFRB) from different sources and different processing technology fed to growing pigs using indirect calorimetry. Thirty-six growing barrows (30.7 ± 3.9 kg) were randomly allotted to 1 of 6 diets with 6 replicate pigs per diet. Diets included a corn-soybean meal basal diet and 5 test diets containing 30% DFRB, respectively. These five samples come from 4 different provinces (i.e., Heilongjiang, Jiangsu, Jilin, and Liaoning province within China) and two of them with the same origin but different processi

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Moon, Ji-Hoi, Cheul Kim, Hee-Su Lee, Sung-Woon Kim, and Jin-Yong Lee. "Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia." Journal of Medical Microbiology 62, no. 9 (2013): 1307–16. http://dx.doi.org/10.1099/jmm.0.053553-0.

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Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml−1) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the

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Okoh, G. R., H. M. Kazeem, G. S. N. Kia, and S. Mailafia. "Evaluation of Enzyme Linked Immuno-Sorbent Assay and Rapid Immuno-Diagnostic Test for Rabies Antigen Detection in Archived Dog Brain Tissues." Folia Veterinaria 62, no. 1 (2018): 18–24. http://dx.doi.org/10.2478/fv-2018-0003.

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Abstract Rabies urgently requires strengthening of new and existing diagnostic methodology in order to overcome the threat it poses. We evaluated the Enzyme Linked Immuno-Sorbent Assay (ELISA) and the Rapid Immunodiagnostic Test (RIDT) in detecting rabies viral antigens, comparing both tests with the Direct Fluorescent Antibody Test (DFAT) which is the gold standard in rabies diagnosis. Fifty dog brain tissues collected from the archives of the Central Diagnostic Laboratory, National Veterinary Research Institute, Vom, Nigeria, were utilized for this study. ELISA performed better than RIDT and

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Schrama, Johan W., Mahmoud N. Haidar, Inge Geurden, Leon T. N. Heinsbroek, and Sachi J. Kaushik. "Energy efficiency of digestible protein, fat and carbohydrate utilisation for growth in rainbow trout and Nile tilapia." British Journal of Nutrition 119, no. 7 (2018): 782–91. http://dx.doi.org/10.1017/s0007114518000259.

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AbstractCurrently, energy evaluation of fish feeds is performed on a digestible energy basis. In contrast to net energy (NE) evaluation systems, digestible energy evaluation systems do not differentiate between the different types of digested nutrients regarding their potential for growth. The aim was to develop an NE evaluation for fish by estimating the energy efficiency of digestible nutrients (protein, fat and carbohydrates) and to assess whether these efficiencies differed between Nile tilapia and rainbow trout. Two data sets were constructed. The tilapia and rainbow data set contained, r

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Mikrogeorgiou, Alkisti, Yoshiaki Sato, Taiki Kondo, et al. "Dedifferentiated Fat Cells as a Novel Source for Cell Therapy to Target Neonatal Hypoxic-Ischemic Encephalopathy." Developmental Neuroscience 39, no. 1-4 (2017): 273–86. http://dx.doi.org/10.1159/000455836.

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Neonatal hypoxic-ischemic (HI) encephalopathy (HIE) remains a major cause of mortality and persistent neurological disabilities in affected individuals. At present, hypothermia is considered to be the only applicable treatment option, although growing evidence suggests that cell-based therapy might achieve better outcomes. Dedifferentiated fat (DFAT) cells are derived from mature adipocytes via a dedifferentiation strategy called ceiling culture. Their abundance and ready availability might make them an ideal therapeutic tool for the treatment of HIE. In the present study, we aimed to determin

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Mo, Rongli, Na Zhang, Jinxin Li, et al. "Transcriptomic Analysis Provides Insights into Anthocyanin Accumulation in Mulberry Fruits." Horticulturae 8, no. 10 (2022): 920. http://dx.doi.org/10.3390/horticulturae8100920.

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Mulberry fruits are rich in anthocyanins, which are important secondary metabolites that give mulberries their bright color, favorable taste and high nutritional quality, making them a popular fruit. However, few studies have focused on the molecular mechanism underlying anthocyanin accumulation in mulberries and the gene regulatory networks of anthocyanin biosynthetic pathways remain largely unknown. In this study, we performed RNA sequencing to identify differentially expressed genes (DEGs) associated with anthocyanin accumulation between two mulberry genotypes (‘Zi Jing’, ZJ and ‘Zhen Zhu B

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Silva, Vanessa, Cláudia Alfarela, Manuela Caniça, et al. "A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers." Antibiotics 11, no. 3 (2022): 374. http://dx.doi.org/10.3390/antibiotics11030374.

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Donkeys (Equus asinus) are in decline in Europe. Occupational exposure to farm animals has been associated with increased staphylococci carriage. We aimed to isolate S. aureus and coagulase-negative staphylococci (CoNS) from donkeys and handlers and characterize the antimicrobial resistance profiles and genetic lineages of S. aureus strains. Oral and nasal swab samples were collected from 49 Miranda donkeys and 23 handlers from 15 different farms. Staphylococci species were identified by MALDI-TOF MS. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR.

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Figiel-Dabrowska, Anna, Klaudia Radoszkiewicz, Paulina Rybkowska, Natalia Ewa Krzesniak, Dorota Sulejczak, and Anna Sarnowska. "Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue." Cells 10, no. 6 (2021): 1475. http://dx.doi.org/10.3390/cells10061475.

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Currently, the number of stem-cell based experimental therapies in neurological injuries and neurodegenerative disorders has been massively increasing. Despite the fact that we still have not obtained strong evidence of mesenchymal stem/stromal cells’ neurogenic effectiveness in vivo, research may need to focus on more appropriate sources that result in more therapeutically promising cell populations. In this study, we used dedifferentiated fat cells (DFAT) that are proven to demonstrate more pluripotent abilities in comparison with standard adipose stromal cells (ASCs). We used the ceiling cu

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Murata, Yasutaka, Daisuke Obinata, Taro Matsumoto, et al. "Urethral injection of dedifferentiated fat cells ameliorates sphincter damage and voiding dysfunction in a rat model of persistence stress urinary incontinence." International Urology and Nephrology 54, no. 4 (2022): 789–97. http://dx.doi.org/10.1007/s11255-021-03083-3.

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Abstract Purpose Dedifferentiated fat (DFAT) cells are mature adipocyte-derived multipotent cells that can be applicable to cell-based therapy for stress urinary incontinence (SUI). This study developed a persistence SUI model that allows long-term evaluation using a combination of vaginal distention (VD) and bilateral ovariectomy (OVX) in rats. Then, the therapeutic effects of DFAT cell transplantation in the persistence SUI model was examined. Methods In total, 48 Sprague–Dawley rats were divided into four groups and underwent VD (VD group), bilateral OVX (OVX group), VD and bilateral OVX (V

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Kra, Gitit, Jayasimha Rayalu Daddam, Hadar Gabay, Sara Yosefi, and Maya Zachut. "Antioxidant Resveratrol Increases Lipolytic and Reduces Lipogenic Gene Expression under In Vitro Heat Stress Conditions in Dedifferentiated Adipocyte-Derived Progeny Cells from Dairy Cows." Antioxidants 10, no. 6 (2021): 905. http://dx.doi.org/10.3390/antiox10060905.

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Heat stress (HS) induces oxidative stress by increasing reactive oxygen species (ROS), and the polyphenol resveratrol (RSV) has been shown to have antioxidant properties by reducing ROS. Hence, we aimed to examine the effects of RSV, HS and their interaction on bovine adipocytes. We generated bovine dedifferentiated adipocyte-derived progeny (DFAT) cells from subcutaneous adipose tissue and examined the effects of RSV (100 µM), heat conditions: isothermal (ISO-37 °C), short heat (SH-41.2 °C for 1 h) and long HS (LH-41.2 °C for 16 h), and their interaction on gene expression in DFAT-cells. In m

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Bui, David Pham, Kathryn Gibb, Martha Fiellin, et al. "Occupational COVID-19 Exposures and Illnesses among Workers in California—Analysis of a New Occupational COVID-19 Surveillance System." International Journal of Environmental Research and Public Health 20, no. 13 (2023): 6307. http://dx.doi.org/10.3390/ijerph20136307.

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Little is known about occupational SARS-CoV-2 exposures and COVID-19 outcomes. We established a Doctor’s First Reports of Occupational Injury or Illness (DFR)-based surveillance system to study cases of work-related COVID-19 exposures and disease. The surveillance data included demographics, occupation, industry, exposure, and illness, details including hospitalization and lost work. We classified workers into ‘healthcare’, non-healthcare ‘public-facing’, or ‘other’ worker groups, and rural–urban commuting areas (RUCAs). We describe worker exposures and outcomes overall by worker group and RUC

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Linsaenkart, Pichchapa, Warintorn Ruksiriwanich, Pensak Jantrawut, et al. "Natural Melanogenesis Inhibitor, Antioxidant, and Collagen Biosynthesis Stimulator of Phytochemicals in Rice Bran and Husk Extracts from Purple Glutinous Rice (Oryza sativa L. cv. Pieisu 1 CMU) for Cosmetic Application." Plants 12, no. 4 (2023): 970. http://dx.doi.org/10.3390/plants12040970.

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Oryza sativa L. cv. Pieisu 1 CMU (PES1CMU) has a high anthocyanin content in the colored bran and high phenolic content in the husk. Biologically active compounds in plants are available as dietary supplements and cosmetics. To expand the utilization of natural resources, PES1CMU will be a natural remedy for skin hyperpigmentation and aging. Cell-free tyrosinase inhibition and scavenging assays were used to screen all extracts, including PES1CMU-rice bran oil (RBO), PES1CMU-defatted rice bran (DFRB), and PES1CMU-husk (H). PES1CMU extracts were first examined in IBMX-stimulated B16 cells and H2

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Yin, Linfei, Lulin Zhao, Tao Yu, and Xiaoshun Zhang. "Deep Forest Reinforcement Learning for Preventive Strategy Considering Automatic Generation Control in Large-Scale Interconnected Power Systems." Applied Sciences 8, no. 11 (2018): 2185. http://dx.doi.org/10.3390/app8112185.

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To reduce occurrences of emergency situations in large-scale interconnected power systems with large continuous disturbances, a preventive strategy for the automatic generation control (AGC) of power systems is proposed. To mitigate the curse of dimensionality that arises in conventional reinforcement learning algorithms, deep forest is applied to reinforcement learning. Therefore, deep forest reinforcement learning (DFRL) as a preventive strategy for AGC is proposed in this paper. The DFRL method consists of deep forest and multiple subsidiary reinforcement learning. The deep forest component

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Yuuki, Yuriko, Takeshi Katafuchi, Tomohiko Kazama, Taro Matsumoto, and Makoto Makishima. "C3H10T1/2 Mesenchymal Stem Cell Line as a New In Vitro Tool for Studying Adipocyte Dedifferentiation." Biology 14, no. 4 (2025): 444. https://doi.org/10.3390/biology14040444.

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Dedifferentiated fat (DFAT) cells are adipocyte-derived cells that are able to differentiate into multiple cell lineages such as adipocytes, osteoblasts and chondrocytes, similar to mesenchymal stem cells (MSCs). Despite their great potential for developing novel clinical interventions by using their multipotency, the detailed mechanisms of how adipocytes undergo dedifferentiation into DFAT cells are not completely understood, because useful in vitro tools for studying adipocyte dedifferentiation are missing. In this study, we show that mature adipocytes derived from the MSC cell line C3H10T1/

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