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Albenzio, Marzia, and Mariangela Caroprese. "Differential leucocyte count for ewe milk with low and high somatic cell count." Journal of Dairy Research 78, no. 1 (2010): 43–48. http://dx.doi.org/10.1017/s0022029910000798.
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This study was undertaken to compare flow cytometry (FC) and direct microscopic leucocyte count (MDLC) for the differentiation of macrophages, lymphocytes and polymorphonuclear leucocyte (PMN) and to evaluate leucocyte distribution in ewe milk with low and high somatic cell count (SCC). Milk samples were grouped for somatic cell count in low SCC (LSCC) when the content was lower than 5·00×105/ml and high SCC (HSCC) when the content was higher than 1·00×106/ml. No differences were found between the two methods tested suggesting that FC could be used as a routine test for rapid discrimination of leucocytes. Percentages of lymphocytes in ewe milk were higher in LSCC (50%) than in HSCC (39%) and count ranged from 273·91±56·62×103cells/ml (LSCC) to 308·90±46·15×103cells/ml (HSCC). PMN number was lower in LSCC than in HSCC (248·83±46·87×103cells/ml v. 444·38±58·62×103cells/ml); accordingly the percentage was lower in LSCC (40%) than in HSCC (57%). No differences were found for macrophages which were 36·36±5·51×103cells/ml and 39·32±6·83×103cells/ml in LSCC and HSCC, respectively. Lymphocytes in ewe milk did not vary with increased number of somatic cells and were the predominant cell type in LSCC. PMN represented the main population detected in HSCC and the correlation with SCC evidenced that this leucocyte class could be useful in differentiating ewe milk cell count, being strictly responsible for the SCC increase.
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Adetula, Adeyinka Abiola, Xiangdong Liu, Thuy Nhien Tran Thi, et al. "Transcriptional Profiling of Leucocyte Count Variation from Porcine Peripheral Blood Reveals Differential Gene Expression." BioMed Research International 2018 (November 18, 2018): 1–11. http://dx.doi.org/10.1155/2018/1496536.
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Leucocytes have tremendous health-check importance related to the individual antiviral capacity of pigs and other mammals. However, the molecular mechanism of the immune response of blood leucocytes in pigs is not completely known. This study investigated the leucocyte-count variation before and after poly I:C stimulation in a Duroc–Erhualian F2 population. Pigs with increased and decreased differences in leucocyte counts were coded as increased responder (IR) and decreased responder (DR), respectively. Then, we used microarray technology to compare the gene-expression profiles of both groups of pigs. Transcriptomic analysis identified 129 differentially expressed genes (DEGs) in IR pigs and 136 DEGs in DR pigs. Forty-one common DEGs showed that both groups had similar expression patterns of immune responses. These results illustrated a differential expression in both groups. Furthermore, qPCR experiment was performed to verify the differential-expression profile. Functional annotation of the DEGs indicated that both IR and DR pigs were similar in several biological processes, including innate immune response, and also exhibited distinct differences in biological processes, molecular function, and pathways. These results provided insights into the mechanism underlying the antiviral capacity of pigs.Trial registration numberis CAS Registry Number24939-03-5.
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Lowe-Jinde, L. "Hematological changes in Cryptobia-infected rainbow trout (Salmo gairdneri)." Canadian Journal of Zoology 64, no. 6 (1986): 1352–55. http://dx.doi.org/10.1139/z86-201.
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Rainbow trout, Salmo gairdneri, were experimentally infected with Cryptobia salmositica and total and differential erythrocyte and leucocyte abundances were examined. Total erythrocyte counts were profoundly reduced and were lowest at 4 weeks postinoculation during the peak of infection. Differential erythrocyte number showed significant reduction in mature erythrocytes, degenerating erythrocytes, and erythroblasts. The reduction in mature erythrocytes number was observed from 3 through 8 weeks postinoculation, while erythroblast reduction occurred at 1 through 5 weeks postinoculation. Subsequently, the number of erythroblasts increase and reach control values at 6, 7, and 8 weeks postinoculation. Although the total leucocyte count was not significantly changed, the differential leucocyte abundance showed that granulocytes were significantly increased. Similarly, the rarely seen, circulating, immature granuloblasts were increased. Both granuloblast and granulocyte numbers returned to control values by 8 weeks postinoculation. Thus, the pattern of differential erythrocytic and leucocytic changes reflect an acute parasitemia and a subsequent partial recovery of the infected fish to the parasitemia.
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Sofyanita, Eko Naning, Hendra Adistia Bangkit, and Roni Afriansya. "Hubungan Jumlah Eritrosit dan Hitung Jenis Leukosit pada Masyarakat dengan Paparan Polutan Tinggi di Desa Tambak Mulyo Kota Semarang." Borneo Journal of Medical Laboratory Technology 3, no. 2 (2021): 217–22. http://dx.doi.org/10.33084/bjmlt.v3i2.2230.
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Lead (Pb) and carbon monoxide (CO) are gas components in airborne pollutants due to vehicle fumes or industrial exhaust gases that affect the blood, namely shortening the life span of erythrocytes produced in erythropoiesis. In addition, small pollutants can be inhaled and enter the alveoli and then phagocytosis and trigger the release of mediators, namely leukocyte cells, which can cause inflammation. This study aims to determine the correlation between the erythrocytes count and differential leucocyte count of the Tambak Mulyo village community, Semarang City. This research was a cross-sectional analytic study of primary data using 20 blood samples, carried out by the erythrocytes count and differential leucocyte count tests. The examination results showed that the average erythrocytes count in all samples was 5.25 million cells/mm3 with a percentage of the normal erythrocytes count is 45.00%, and a high of erythrocytes count is 55.0%. On the test of the differential leucocyte count, 4 samples are obtained with specific values. Normal leukocytes and as many as 16 samples experienced an increase and decrease in the differential leucocyte count. The chi-square test shows Asymp.Sig> 0.05, which means there is no correlation between variables, so it can be concluded that there is no significant correlation between the erythrocytes count and differential leucocyte count (lymphocytes, basophils, eosinophils, neutrophils, and monocytes) in the community with exposure to high pollutants in Tambak Mulyo village, Semarang City.
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Ava, Dihingia, and Jahan Wasima. "A Study Of The Hematological Profile In Relation To Some Allergic Diseases (A Hospital Based Study)." International Journal of Basic and Applied Physiology 2, no. 1 (2013): 35–40. https://doi.org/10.5281/zenodo.4478485.
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Eosinophils and neutrophils play major roles in pathogenesis of allergy.However the relation between peripheral blood cell counts of other major leukocyte groups, Hb%, ESR in allergic diseases is less clear.Therefore this study was conducted to find out if there is any variation in the haematological profile in subjects having allergic disease. Method: 50 cases of bronchial asthma, 50 cases of some allergic skin disorders and 50 cases of allergic rhinitis and 50 controls were chosen for this study. The study design was cross-sectional. Total Leucocyte Count, Absolute Eosinophil Count were done using Neubauer’s chamber, Differential Leucocyte Count by Leishman’s staining and Erythrocyte Sedimentation Rate using Westergren’s method. Haemoglobin estimation was done by cyanmethaemoglobin method. The statistical analysis was done using one way analysis of variance (ANOVA) followed by t test. Result: The results showed a significant increase (p<0.05) in the Total Leucocyte Count, Absolute Eosinophil Count, Erythrocyte Sedimentation Rate and the differential counts of neutrophil and eosinophil, and a significant decrease (p<0.05) in the lymphocyte count among the cases as compared to the controls. Conclusion: It is seen that in allergic condition besides an increase in the eosinophil count, other haematological parameters also change.
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Sultana, Safia, Nabila Afsar, Mohammed Jawad, and Mohammed Abdul Hannan Hazari. "Effects of cigarette smoking on erythrocyte sedimentation rate, platelet count, total and differential leucocyte counts in adult male smokers." Annals of Medical Physiology 3, no. 1 (2019): 14–18. https://doi.org/10.23921/amp.2019v3i1.35185.
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Smoking is one of the leading causes of death worldwide. Smokers have higher risk for coronary heart disease, atherosclerosis, acute myocardial infarction, hypertension, clotting disorders, inflammation, respiratory diseases, cancers, etc. A cigarette smoker is exposed to a number of harmful substances. In this study we hypothesized that smoking causes inflammatory reactions and induces hyperthrombic state in the body which may be reflected in erythrocyte sedimentation rate (ESR), total leucocyte count (TLC), differential leucocyte count (DLC) and platelet count values. The purpose of the study was to study the effects of cigarette smoking on erythrocyte sedimentation rate, total leucocyte count and platelet count in adult male smokers and to compare the results with non-smokers and to establish a relationship between the duration and quantity of smoking with the change in ESR, TLC, DLC and platelet count. A cross sectional study was conducted in the department of Pathology on 86 healthy male subjects (smokers=43 and non-smokers=43). ESR was estimated using Westergrens method. TLC, DLC and platelet counts were estimated using HORIBA Pentra ES60 autoanalyser. TLC and basophil counts were significantly higher in smokers than in non-smokers (p<0.05). The mean value of ESR was higher among smokers than non-smokers but it was statistically insignificant. Platelets counts showed no significant difference between smokers and non- smokers. No correlation was observed in various blood parameters and smoking (in pack years). We conclude that smoking initiates an inflammatory response as evidenced from raised TLC, monocyte and basophil counts.
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Sultana, Safia, Nabila Afsar, Mohammed Jawad, and Mohammed Abdul Hannan Hazari. "Effects of cigarette smoking on erythrocyte sedimentation rate, platelet count, total and differential leucocyte counts in adult male smokers." Annals of Medical Physiology 3, no. 1 (2019): 14–18. http://dx.doi.org/10.23921/amp.2019v3i1.35185.
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Smoking is one of the leading causes of death worldwide. Smokers have higher risk for coronary heart disease, atherosclerosis, acute myocardial infarction, hypertension, clotting disorders, inflammation, respiratory diseases, cancers, etc. A cigarette smoker is exposed to a number of harmful substances. In this study we hypothesized that smoking causes inflammatory reactions and induces hyperthrombic state in the body which may be reflected in erythrocyte sedimentation rate (ESR), total leucocyte count (TLC), differential leucocyte count (DLC) and platelet count values. The purpose of the study was to study the effects of cigarette smoking on erythrocyte sedimentation rate, total leucocyte count and platelet count in adult male smokers and to compare the results with non-smokers and to establish a relationship between the duration and quantity of smoking with the change in ESR, TLC, DLC and platelet count. A cross sectional study was conducted in the department of Pathology on 86 healthy male subjects (smokers=43 and non-smokers=43). ESR was estimated using Westergrens method. TLC, DLC and platelet counts were estimated using HORIBA Pentra ES60 autoanalyzer. TLC and basophil counts were significantly higher in smokers than in non-smokers (p<0.05). The mean value of ESR was higher among smokers than non-smokers but it was statistically insignificant. Platelets counts showed no significant difference between smokers and non- smokers. No correlation was observed in various blood parameters and smoking (in pack years). We conclude that smoking initiates an inflammatory response as evidenced from raised TLC, monocyte and basophil counts.
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Strahm, Carol, Vilijam Zdravkovic, Claus Egidy, and Bernhard Jost. "Accuracy of Synovial Leukocyte and Polymorphonuclear Cell Count in Patients with Shoulder Prosthetic Joint Infection." Journal of Bone and Joint Infection 3, no. 5 (2018): 245–48. http://dx.doi.org/10.7150/jbji.29289.
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Abstract. Joint aspiration in suspected infected implants is a validated diagnostic method in establishing the diagnosis of prosthetic joint infection (PJI). Cut-off values for synovial leukocyte counts and differentials are well described for patients with hip and knee PJI. In 19 failed shoulder implants, a leucocyte count of >12.2 G/L had a sensitivity and specificity of 92% and 100% respectively; A differential of >54% neutrophils had a sensitivity of 100 % and a specificity of 75%.
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Mercelina-Roumans, P. E. A. M., J. M. H. Ubachs, and J. W. J. van Wersch. "Leucocyte count and leucocyte differential in smoking and non-smoking females during pregnancy." European Journal of Obstetrics & Gynecology and Reproductive Biology 55, no. 3 (1994): 169–73. http://dx.doi.org/10.1016/0028-2243(94)90033-7.
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Ojha, AR, and UR Aryal. "Leucocytosis in Febrile Seizure." Journal of Nepal Paediatric Society 31, no. 3 (2011): 188–91. http://dx.doi.org/10.3126/jnps.v31i3.4577.
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Introduction: Febrile seizure (FS) is a common condition affecting 2-5% of children. Peripheral blood leucocyte count with its differential is an initial test looking for the cause of fever and high count is usually accounted for the seizure activity. Objectives: To find the incidence of febrile seizure and to evaluate the relationship between fever duration before seizure, seizure duration and the total leucocyte as well as neutrophil response. Methodology: It is a cross sectional study done at Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal, for one year. After an informed consent, children from 6 months to 6 years with FS were admitted. Those with afebrile seizures and who refused to give consent were excluded. A detail history and examination was done on admission. All were investigated for the source of fever including total and differential count. Results: Out of a total 1742 children admitted, 115 (6.6%) children had febrile seizure. Simple febrile seizure was observed in 93(81%) and 22(19%) was complex febrile seizure. 42% of them had leucocytosis. The duration of fever before the onset of seizure is found to be negatively correlated with total leucocyte count (r = -0.418, p<0.001) and neutrophils count alone (r = -0.375, p<0.001). The duration of seizure is not correlated to both the total leucocyte count (r = -0.162, p = 0.85) and the neutrophil (r = -0.109, p= 0.247). Conclusion: The incidence of febrile seizure is 6.6%. Leucocytosis and neutrophilia in children is negatively correlated with the duration fever before the onset of seizure, associated with underlying infection if any and is not related to seizure event and its duration. Thus any child with febrile seizure with high leucocyte count should be evaluated for infection. Key words: Fever; Leucocytosis; Neutrophilia; Seizure DOI: http://dx.doi.org/10.3126/jnps.v31i3.4577 J Nep Paedtr Soc 2011;31(3):188-191
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Alifiah, Nabila, Siemona L. E. Berhimpon, and Glady I. Rambert. "Description of Leucocyte Differential Count in Pregnant Women with COVID-19." Medical Scope Journal 3, no. 2 (2022): 151. http://dx.doi.org/10.35790/msj.v3i2.37841.
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Abstract: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a high rate of transmission. Pregnant women are at high risk of infection due to the changes in their physiological and immune systems. This study aimed to determine the leukocyte differential count leukocyte in pregnant women with COVID-19. This was a descriptive study with a retrospective approach, using secondary data of the patients’ medical records during the periode of July-August 2021 at Prof. Dr. R. D. Kandou Hospital, Manado. The results showed that of 57 pregnant women with COVID-19, 92% had normal leukocyte differential count, 100% had normal basophil and eosinophil counts, 12% had neutrophilia, 23% had lymphopenia, and 95% had normal monocyte counts. In conclusion, in pregnant women with COVID-19 at Prof. Dr. R. D. Kandou Hospital, the most common abnormalities in leucocyte differential count are lymphopenia and neutrophiliaKeywords: pregnant women; COVID-19; leukocyte differential count Abstrak: Coronavirus disease 2019 (COVID-19) yang disebabkan oleh severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mempunyai tingkat penularan yang sangat tinggi. Wanita hamil berisiko tinggi terinfeksi COVID-19 karena adanya perubahan fisiologik dan sistem imun. Penelitian ini bertujuan untuk mengetahui gambaran hitung jenis leukosit pada wanita hamil dengan COVID-19. Jenis penelitian yang digunakan ialah deskriptif retrospektif dengan menggunakan data sekunder rekam medik periode Juli-Agustus 2021 di RSUP Prof. Dr. R. D. Kandou. Hasil penelitian memperlihatkan bahwa dari 57 wanita hamil terkonfirmasi COVID-19, terdapat 92% dengan nilai hitung leukosit normal, 12% mengalami neutrofilia, 23% mengalami limfopenia, 95% memiliki nilai hitung monosit normal, dan 100% memiliki nilai hitung basofil dan eosinofil normal. Simpulan penelitian ini ialah kelainan hitung jenis leukosit yang paling sering ditemukan pada wanita hamil terkonfirmasi COVID-19 di RSUP Prof. Dr. R. D. Kandou ialah neutrofilia dan limfopenia.Kata kunci: wanita hamil; COVID-19; hitung jenis leukosit
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Chantereau, S., and C. Sultan. "Evaluation of the interest in ordering the leucocyte differential count." Social Science & Medicine 21, no. 10 (1985): 1177–79. http://dx.doi.org/10.1016/0277-9536(85)90176-5.
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Wijaya, Sumi, and Monica S.W. "UJI EFEK ANTIINFLAMASI EKSTRAK HERBA SURUHAN (Peperomia pellucida L. KUNTH) PADA TIKUS PUTIH JANTAN." Berkala Penelitian Hayati 9, no. 2 (2004): 115–18. http://dx.doi.org/10.23869/bphjbr.9.2.20049.
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Peperomia pellucida L. Kunth herbs (Herba Suruhan) is one of the alternative for inflamation therapy. In this study, albino rats were devided into seven groups. The first three groups was administered orally the extract of Suruhan herbs 1500 mg/Kg body weight, 2000 mg/Kg body weight and 2500 mg/Kg body weight. The next three groups were given Indomethacine solutions with the doses of 13.5 mg/Kg body weight, 15.8 mg/Kg body weight and 15 mg/Kg body weight, and the last groups (control) were given 1 percent of PGA suspension alone. Fifty microliter of Carrageen solution of 1.5 percent was injected subcutaneously to all rats in those group. The volume of the foot paw of albino male rat injected with carrageen, was measured and recorded for 4 hours. The number of leucocyte and the number of differential count were observed every two hour, namely at the fourth, sixth and eight hours following carrageen injection. There were significant differences in the antiinflamation effect using plethysmometer and the number of leucocyte counts between control and all the treatment group. For the differential count, not all the results showed significant differences.
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B.V., Swaroop Raj, Divya C., and Smitha B.V. "Image Analysis and Image Classifier Using Neural Network with Machine Learning to Perform Differential Leucocyte Count." Indian Journal of Pathology: Research and Practice 7, no. 6 (2018): 772–75. http://dx.doi.org/10.21088/ijprp.2278.148x.7618.11.
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S., Soujanya1* D. Madhuri2 J. Jyothi3. "Cytological diagnosis and haematological alterations in transmissible venereal tumour." Science World a monthly e magazine 3, no. 9 (2023): 1240–45. https://doi.org/10.5281/zenodo.8370687.
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The study was conducted in 12 non-descriptive dogs (8 female and 4 male) with nodular tumour growths on external genitalia which were presented to Veterinary Clinical Complex, Rajendranagar, Hyderabad, Telangana for treatment. Impression smears were taken from the tumour mass and stained with Fields stain for cytological diagnosis. Cytologically, the tumours revealed homogenous highly cellular mass. The neoplastic cells were large, round with multiple punctate vacuoles in cytoplasm and the nuclear chromatin was coarse to reticulate which were suggestive of transmissible venereal tumour. After diagnosis the affected dogs were treated with Vincristin. Further blood samples were collected and analyzed for various haematological parameters. In dogs, after treatment with Vincristin the total erythrocyte count, hemoglobin, packed cell volume, total leucocyte count and platelet count were significantly (P<0.05) decreased in comparison with before treatment. In differential leucocytic count, the neutrophil percentage was significantly (P<0.05) decreased and lymphocytic percentage was significantly (P<0.05) increased in dogs after treatment in comparison with before treatment.
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Haider, Rana Zeeshan, Najeed Ahmed Khan, Eloisa Urrechaga, and Tahir Sultan Shamsi. "Mature and Immature/Activated Cells Fractionation: Time for a Paradigm Shift in Differential Leucocyte Count Reporting?" Diagnostics 11, no. 6 (2021): 922. http://dx.doi.org/10.3390/diagnostics11060922.
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Leucocytes, especially neutrophils featuring pro- and anti-cancerous characteristics, are involved in nearly every stage of tumorigenesis. Phenotypic and functional differences among mature and immature neutrophil fractions are well reported, and their correlation with tumor progression and therapy has emerging implications in modern oncology practices. Technological advancements enabled modern hematology analyzers to generate extended information (research parameters) during complete blood cell count (CBC) analysis. We hypothesized that neutrophil and lymphocyte fractions-related extended differential leucocytes count (DLC) parameters hold superior diagnostic utility over routine modalities. The present study was carried out over a four-and-a-half-year period wherein extended neutrophil (immature granulocyte [IG] and mature neutrophil [NEUT#&]), and lymphocyte (activated/high fluorescence lymphocyte count [HFLC] and resting lymphocyte [LYMP#&]) parameters were challenged over routine neutrophil [NEUT#] and lymphocyte [LYMP#] items in a study population of 1067 hematological neoplasm patients. Extending the classical statistical approaches, machine-learning-backed data visualization was used to explore trends in the study parameters. As a whole, extended neutrophil and lymphocyte count outperformed and was diagnostically more relevant than routine neutrophil and lymphocyte parameters by showing the least difference from their respective (gold-standard) manual DLC counts. The mature neutrophil count was compared to IG, and resting lymphocyte count was compared to HFLC by calling the function ‘correlation’ as a ‘clustering function’ for heatmap based visualization. The aforementioned study parameters displayed close clustering (rearrangement) for their respective study items by presenting distinct trends of equally valuable weights (deviated values), advocating fractions-based extended DLC reporting. Importantly, using a Bland and Altman analysis analogously to a manual neutrophil count, the mature neutrophil count [NEUT#&] remained unbiased since a routine neutrophil count [NEUT#] was found to be a negatively biased. The extended DLC-parameter-driven fractions-based reporting has superior diagnostic utility over classical routine approaches; this finding can largely minimize labor-intensive manual DLC practices, especially in hematology–oncology departments.
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R, Mark, and Jessica Rogge. "Early detection of COVID-19 using characteristic leucocyte differential count (CLDC)." Life Research 3, no. 3 (2020): 101. http://dx.doi.org/10.53388/life2020-0712-102.
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K, Vipina, and Ambika V. "COMPARATIVE STUDY OF DIFFERENTIAL LEUCOCYTE COUNT BY MANUAL AND AUTOMATED METHOD." Journal of Evolution of Medical and Dental Sciences 7, no. 03 (2018): 328–31. http://dx.doi.org/10.14260/jemds/2018/73.
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Yonus, Nadia, Asma Basharat Ali, Shaikh Sajjad, Sahrish Mukhtar, Aaqiba Rasheed, and Tayyaba Kazmi. "Changes in Hematological Parameters of Sprague Dawley Rats with Use of Cyclosporine and Nigella Sativa." ANNALS OF ABBASI SHAHEED HOSPITAL AND KARACHI MEDICAL & DENTAL COLLEGE 25, no. 4 (2021): 211–17. http://dx.doi.org/10.58397/ashkmdc.v25i4.412.
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Objective: The objective of this study was to observe the signs of Cyclosporine toxicity and to analyze the ameliorative effects of Nigella Sativa (NS or Kalonji) on body weight and haematological parameters.Methods: This study was conducted in the Department of Anatomy, Baqai Medical University of Karachi during the month of February 2017. Thirty healthy male Sprague Dawley rats were selected from the Animal House of the university. After acclimatization, the animals were randomly divided into three groups, with 10 rats in each Group. Group A was the control group and received no intervention. Group B received treatment of oral Cyclosporine (15 mg/kg/day) in a single daily dose and Group C was given Cyclosporine (15 mg/kg/day) with Nigella Sativa seeds (450 mg/kg) as a protective agent. The bodyweight of rats in each group was measured at the start of the study period. The treatment was given for the duration of 21 days, after which the body weight was measured again. The rats were anaesthetized and blood was collected through cardiac puncture. Blood analysis for haemoglobin percentage, total leucocyte count and differential leucocyte count was done using haematology analyzer. Manual differential leucocyte was also done after staining the blood smear with Leishman's stain.Results: Final body weight of rats in Cyclosporine treated Group B was decreased significantly p<0.001 in comparison to control Group A and increased significantly p=0.001 in Nigella Sativa and Cyclosporine protected Group C. Blood parameters showed decreased mean haemoglobin p=0.03, decreased mean total leucocyte count p=0.003, increased mean neutrophil count p=0.27 and decreased mean lymphocyte count p=0.27 in Cyclosporine treated Group B while the mean values of these haematological parameters improved in Nigella Sativa protected Group C.Conclusion: The present study demonstrated that Cyclosporine caused a decrease in animal weight and toxic changes in haematological parameters while Nigella Sativa was helpful in ameliorating them
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Tan, Guat Bee, Christina Sum, and Ponnudurai Kuperan. "New Monoclonal Antibodies Cocktail for the Flow Cytometry Leukocyte Differential Method." Blood 116, no. 21 (2010): 4726. http://dx.doi.org/10.1182/blood.v116.21.4726.4726.
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Abstract Abstract 4726 Automated blood cell counters provide a leucocyte count and five-part (some 6-part) leucocyte differential, however haematology instrument differentials provide only limited information on cell morphology using abnormal cell flags and are often unable to reliably classify abnormal and immature cells. There are also limitations with the standard microscopic differential, identification of cells is subjective and there is significant inter and intra observer variation (Koepke et al. 1985). It is also subject to significant statistical variance (Rumke 1985). There have recently been reports of using monoclonal antibody cocktails for an extended leucocyte differential by flow cytometry. The International Council for Standardization in Haematology has set up a group to prepare an international reference method for an extended flow differential; this is intended to replace the current reference manual microscopic 2 × 200 cell count (CLSI H20-A2). Currently, there are several different protocols in use for leucocyte differential using different monoclonal antibodies and gating strategies. The aim of this study was to compare the differential results from a protocol from Singapore (SGP) with published flow cytometric protocols for the leucocyte differential from France (Faucher et al. 2007, Roussel et al. 2010) and to the automated count from the Beckman Coulter LH750 analyser and the current reference microscopic method. The French flow cytometric method uses 6 antibodies and allows detection of all white blood cells, mature neutrophils, total lymphocytes, total monocytes, eosinophils, basophils, immature granulocytes, B lymphocytes, non-cytotoxic T-lymphocytes, cytotoxic T/NK lymphocytes, CD16 positive and CD16 negative monocytes, and blasts cells with lineage orientation. The SGP method uses 8 antibodies (CD3, CD34, CD117, CD45, CD13, CD20, CD16, CD56), premixed in single tube. It detects mature neutrophils, total lymphocytes, total monocytes, eosinophils, basophils, CD16 positive and CD16 negative monocytes, T-lymphocytes, B-Lymphocytes, NK-cells, immature granulocytes and blasts. A 5-colour flow cytometer, the Beckman Coulter FC500, was used in this study. EDTA blood was analysed on 27 normal and 148 abnormal samples, either with complete blood count values outside the reference range or which demonstrated abnormal cell flags on the LH750. These samples included blast cells, immature granulocytes and abnormal lymphocytes. Results for most cell populations measured by the SGP flow differential compared well with the LH750, the manual reference method and French protocol. Comparative results using Pearson correlation are presented in Table 1. For the SGP protocol, correlation with the LH750 and with the manual differential was good for neutrophils, lymphocytes, monocytes and eosinophils. Excellent correlation was observed for all cells apart from basophils when the two flow methods were compared to each other. There was no correlation for basophils between the SGP flow method and the manual method. Similarly, there was no correlation between SGP flow method and LH750 nor between both flow methods. This is not surprising as basophils are usually present in very low numbers. Hence, without a positive marker for basophils in the flow cytometric panel correlation may depend on the type of samples used for the evaluation. Very good correlation of blast cells, r=0.99 and immature granulocytes, r=0.88 was seen between the manual and the SGP method. When comparing the flow methods to each other correlation for blast cells shows an r value of 0.96 and immature granulocytes 0.97. Our study shows that this flow cytometric method performs well with both normal and abnormal patient samples. A differential using monoclonal antibodies for immunological recognition of cells provides more information than either the manual or automated differential. In addition to the detection of the common cell populations, blast cells, immature granulocytes, subpopulations of lymphocytes and inflammatory monocytes are enumerated. Disclosures: No relevant conflicts of interest to declare.
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Ghozali, M., Suhendra Praptama, Rini Widyastuti, et al. "Decrease of Peripheral Monocyte Relative Number and Mean Platelet Volume in Iron Overloaded Mice." Biomedical and Pharmacology Journal 12, no. 1 (2019): 443–51. http://dx.doi.org/10.13005/bpj/1659.
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Iron overload (IO) because of multiple blood transfusion as a definite therapy for hematological disease with chronic and severe anemia has become a major concern. Deleterious complication contributed by chemically reactive deregulated iron may affect cellular homeostasis systemically lead to tissue and organ damage. When this toxicity occurred in blood cells, alteration of peripheral hematological profile concerning erythrocyte, leucocyte, and platelet most likely to be modified and imperatively need to be evidenced. The experimental IO mice model was established by injecting a low and high dose of iron dextran intraperitoneally. Peripheral erythrocyte, leucocyte and platelet indices measured by hematology analyzer were analyzed. A dynamic tendency of leucocyte absolute cell number and differential cell count of low and high dose iron treatment and a significant decrease of differential monocyte count were found. In addition, high dose iron treatment showed a significantly lower mean platelet volume. In conclusion, this study verified that IO impaired the cellular hematological indices by selectively suppress monocyte number addressing that this mononuclear phagocyte was the most affected immune cell. Furthermore, low mean platelet volume following acquired platelet function defect was evidenced. This research provided an animal experimental model that could be used for further study in finding alternative therapeutic targets on the pathophysiology of iron overload diseases, such as thalassemia.
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Dhal, Nirjharini, Sridhar Panda, Namita Mohapatra, Naresh C. Pattanayak, and Rohan Pattanaik. "Study of haematological abnormalities in HIV infected patients and its correlation with CD4 counts." International Journal of Research in Medical Sciences 6, no. 9 (2018): 2937. http://dx.doi.org/10.18203/2320-6012.ijrms20183385.
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Background: To study and correlate the haematological abnormalities with CD4 cell counts in HIV infected patients diagnosed on OPD basis in S.C.B Medical College, Cuttack, before they are initiated on Antiretroviral (ARV) therapy.Methods: A 100, ELISA positive, untreated HIV patients were included in the study while those patients with history of any haematological disease, Chronic Kidney Disease (CKD), Chronic Liver Disease (CLD), underlying malignancy or on chemotherapy were excluded. Following clinical evaluation, haemoglobin levels (Hb. %), Total Leucocyte Count (TLC), Differential Leucocyte Count (DLC), Erythrocyte Sedimentation Rate (ESR), Platelet Count (PC), CD4 counts (by flow cytometry) and peripheral smear examination was done. They were staged as per WHO clinical staging guidelines given by NACO and statistical analysis was drawn by Fischer Exact Test and association between CD4 counts and haematological abnormalities were inferred.Results: The mean age of the patients was found to be 36.85±6.2 years with males (63%), married (80%) and rural population (78%) showing commonest mode of transmission of the virus as heterosexual route (94%). Most common clinical finding was found to be pallor (68%) with majority in stage III of AIDS. Mean TLC count was found to be 5872±2210 cells/mm 2.40% had leucopenia on TLC and 29% had neutropenia, 30% lymphocytopenia and 20% monocytopenia on DLC. Mean CD4 count was 89 cells per microliter and 79% were anaemic. CD4 counts did not statistically correlate either with WHO staging or cytopenias or haemoglobin levels. However, there was positive association between CD4 counts with anaemia, WHO stages of AIDS, lymphocytopenia and monocytopenia in this study.Conclusions: In this study we could ascertain that, majority were in WHO stage III of AIDS with CD4 counts <200 cells per microliter and blood findings of various cytopenias and anaemia. From our findings, we could correlate between WHO stage of AIDS, CD4 counts and haematological abnormalities thus, establishing the essence of our study.
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Jadhav, Dhanashri, Gaurav Singh, Madan Mishra, Abhinav Srivastava, and Shubhamoy Mondal. "Comparative evaluation of efficacy of C-reactive protein versus total leucocyte count as marker for monitoring patients with orofacial space infection of odontogenic origin." National Journal of Maxillofacial Surgery 14, no. 3 (2023): 406–12. http://dx.doi.org/10.4103/njms.njms_139_22.
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Objective: To compare and evaluate the efficacy of C-Reactive Protein and total leucocyte count as markers for monitoring patients of odontogenic space infection. Materials and Methods: Blood samples were taken preoperatively and post-operatively at day 1, day 2, day 3 and day 7 for measuring the levels of CRP and TLC. The trends of CRP and TLC were analysed against the Clinical Severity Scale (CSS). The study was carried out on patients of odontogenic space infection, and minimum of 50 patients were selected for this study. Comparative evaluation of C-reactive protein (CRP) versus total leucocyte count (TLC) was performed. Results: Data were analysed using Statistical Package for Social Sciences (SPSS) version 21. Inferential statistics were performed using Spearman Correlation. The level of statistical significance was set at 0.05. The result of the study demonstrates that the C-reactive protein (CRP) was found to be more consistent indicator for monitoring the patients of odontogenic space infection than TLC, Differential Leucocyte Count (DLC) and erythrocyte sedimentation rate (ESR). Conclusion: We conclude that CRP should be incorporated as monitoring tool, and it serves as a better indicator than TLC for managing patients with orofacial space infections of odontogenic origin.
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Tan, Guat Bee, Christina Sum, and Ponnudurai Kuperan. "Assessment of the Analytical Performances of the Flow Leucocyte Differential Method." Blood 116, no. 21 (2010): 4727. http://dx.doi.org/10.1182/blood.v116.21.4727.4727.
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Abstract Abstract 4727 The examination of blood films by microscopy remains one of the major labour intensive procedures in the laboratory and the challenge is to reduce the number of blood films examined without missing important diagnostic information. Automated blood cell counters offer a leucocyte count, red cell and platelet count and five-part (some 6-part) leucocyte differential. Haematology instrument differentials provide only limited information on cell morphology using abnormal cell flags and are often unable to reliably classify abnormal and immature cells. The examination of blood films is not only time consuming, it also requires highly trained staff. The impact of a wrong diagnosis necessitates that experienced staff are present in the laboratory 24 hours a day. Furthermore, manual cell classification is subjective, with significant inter and intra observer variation (Koepke et al. 1985) and is also subject to significant statistical variance (Rumke 1985). There have recently been several reports of using monoclonal antibody cocktails for an extended leucocyte differential by flow cytometry (Faucher et al. 2007, Roussel et al. 2010). The aim of this study was to compare a flow cytometric method for the white blood cell differential with the automated count from the Beckman Coulter LH750 haematology analyser and the reference manual microscopic 2 × 200 cell count (CLSI H20-A2). Cell morphology was also assessed microscopically for the presence of cells such as reactive or abnormal lymphocytes or blasts. The flow cytometric method, described by Faucher et al. 2007, uses 6 antibodies (CD45, CD36, CD2, CD294, CD19 and CD16) premixed in a single tube. The protocol allows detection of all white blood cells, mature neutrophils, total lymphocytes, total monocytes, eosinophils, basophils, immature granulocytes, B lymphocytes, non-cytotoxic T-lymphocytes, cytotoxic T/NK lymphocytes, CD16 positive and CD16 negative monocytes, and blasts cells with lineage orientation. A 5-colour flow cytometer, the Beckman Coulter FC500, was used for analysis. The gating strategy described by Faucher et al. (2007) was used. EDTA blood was analysed on 27 normal samples and 148 abnormal samples which demonstrated abnormal cell flags on the LH750. These samples included the presence of blast cells, immature granulocytes and abnormal lymphocytes. Results for most cell populations measured by the flow cytometric differential compared well with both the LH750 automated differential and the manual reference method. Comparative results using Pearson correlation show that the automated LH750 differential produced r values of greater than 0.94 for neutrophils, lymphocytes and eosinophils. The manual reference method produced r values of greater than 0.89 for neutrophils, lymphocytes and eosinophils. Results for flow cytometric monocytes compared to the LH750 and manual differential gave an r value of 0.84 and 0.87 respectively. Results for basophils were significantly better when the flow cytometric method was compared to the LH750 rather than the manual method, r = 0.68 for flow cytometry versus LH750 and r = 0.43 for flow cytometry versus manual method. The value of the manual differential is diminished because of the low number of cells counted; the precision is not good for smaller cell populations (Hübl et al. 1995). Very good correlation of blast cells, r = 0.98 and immature granulocytes, r = 0.92 was seen between the manual and flow cytometric method. The flow cytometric differential is superior to the microscopic method since it is objective and due to the higher number of cells counted, it can detect subpopulations of cells that are present in smaller number with greater statistical and interpretive confidence. More importantly, it recognises and quantitates morphologically abnormal cells such as reactive lymphocytes, inflammatory monocytes and the lineage of blast cells. However, the examination of blood cell morphology by microscopy still has an important role in the diagnosis of diseases. Disclosures: No relevant conflicts of interest to declare.
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Bhatkar, N. V. "Chromium (III) induced haematological alterations in Indian common carp, Labeo rohita (Ham.)." Journal of Applied and Natural Science 3, no. 2 (2011): 258–63. http://dx.doi.org/10.31018/jans.v3i2.192.
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The principle source of chromium in water bodies is discharged from industries which use large amount of chromates or dichromates. The freshwater fish, Labeo rohita was used as a model in the present studies to investigate the responses to chromium metal salt contamination in water bodies as it is the most common fish consumed widely in India. Haematological tests were carried out as these are the important diagnostic tools and are equally valuable as indicators of disease or stress due to pollutants and environmental fluctuations. The fishLabeo rohita were exposed to sublethal concentration of chromium chloride for 10, 20 and 30 days to study alterations in certain haematological parameters. The parameters under study were total erythrocyte count (TEC), haemoglobin (Hb gm%), total leucocyte count (TLC), differential leucocyte count (DLC) and pack cell volume (PCV). The studies revealed that chromium chloride-treated Labeo rohita exhibited decreased levels of total erythrocyte count (TEC), haemoglobinn (Hb gm%) and PCV whereas, total leucocyte count (TLC) was increased under thestress of chromium metal The decreased WBC count found after 30 days along with depleted Hb content and RBC count indicate dysfunctioning of haemopoietic systems along with dysleucopoiesis. Leucocytosis observed after 10 and 20 days exposure has been considered to be an adaptation to meet stressful conditions by animals. Increase in macrophages and basophils appears to be protective response during chromium exposure. An increasing trend in the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were evident in fish exposed to sublethal doses of chromium chloride when compared with the control.
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Pai Jakribettu, Ramakrishna, Rekha Boloor, Andrew Thaliath, et al. "Correlation of Clinicohaematological Parameters in Paediatric Dengue: A Retrospective Study." Journal of Tropical Medicine 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/647162.
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Dengue is one of the arthropod-borne (arbo) viral diseases transmitted by female mosquitoAedes aegypti. Dengue fever has a wide spectrum of clinical presentation ranging from flu-like illness to severe complicated stage of dengue hemorrhagic fever leading to mortality. This was a retrospective study conducted in a tertiary care hospital in Coastal Karnataka, South India, to know the correlation between the clinical presentation and haematological parameters in the paediatric cases presented with dengue symptoms. A total of 163 paediatric cases who presented fever and dengue-like illness were included in the study. Of which, 69 were confirmed dengue patients. Critical analysis showed that there was a significant difference in the haematological parameters like total leucocyte count, percent differential leucocyte count, and platelets count, in the erythrocyte sedimentation rate (P<0.05to 0.0001). Additionally, when compared to nondengue patients, even the liver function and renal function parameters were significantly deranged (P<0.05to 0.0001). Stratification based on NS1, IgG, and IgM showed significant alterations in the haematological, hepatic, and renal parameters. With respect to the treatment a small percentage of patients, that is, 8% (4 patients), required platelet transfusion as their counts went below 20,000/μL. Two patients succumbed to their illness while three required ICU stay.
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Hendrianingtyas, Meita, and MI Tjahjati DM. "LEUKOSIT INFEKSI BAKTERI SISTEMIK." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 17, no. 3 (2018): 155. http://dx.doi.org/10.24293/ijcpml.v17i3.1094.
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Patients in intensive care unit (ICU) have a high risk for systemic bacterial infection. Serum procalcitonin (PCT) known as a markerto predict bacterial infection, systemic inflammation responsse (SIRS) or sepsis. Another simple and easy indicator is by using leucocytecount-differential count, absolute neutrophyl count (ANC), and immatur/total (I/T) neutrophyl ratio. The aim of this study was toknow the comparation of the leucocyte count-differential count, ANC, and I/T ratio with procalcitonin serum value in patients suspectto systemic bacterial infection at ICU. A cross sectional study on 20 patients suspected with systemic bacterial infection in ICU. The datawas classified in 3 groups based on PCT serum value: group A (< 0.5 ng/mL), group B (0.5–2 ng/mL) and group C (> 2 ng/mL). Thedata was analysed by one way ANOVA test if normally distributed, and by Kruskall-Wallis test if not normally distributed. Significancywas confirmed at p < 0.05. A post hoc and Mann-Whitney test performed on a significant result. The frequency of group A was 3 (15%),group B = 5 (25%), and group C = 12 (60%). There is no significant difference on leucocyte count in 3 groups (p = 0.953), neutrophylI/T ratio (p = 0.259), ANC (p = 0.91), eosinophyl count (p = 0.287), segment neutrophyl (p = 0.094), and monosit (p = 0.152).There was a difference on lymphocyte count (p = 0.01) between group C with group A and group B and there was a difference on staffneutrophyl count (p = 0.029) and total neutrophyl count (p = 0.003) between group A with group B and C (p = 0.029). In this studywere found differences on lymphocyte, staff neutrophyl and total neutropyl count to the PCT value
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Myhre, Erling, Jean Henrik Braconier, and Ulf Sjögren. "Automated Cytochemical Differential Leucocyte Count in Patients Hospitalized with Acute Bacterial Infections." Archives Of Physiology And Biochemistry 17, no. 2 (1985): 201–8. http://dx.doi.org/10.3109/13813458509061667.
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Myhre, Erling B., Jean Henrik Braconier, and Ulf Sjögren. "Automated Cytochemical Differential Leucocyte Count in Patients Hospitalized with Acute Bacterial Infections." Scandinavian Journal of Infectious Diseases 17, no. 2 (1985): 201–8. http://dx.doi.org/10.3109/inf.1985.17.issue-2.12.
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Singh, Shruti, Jaya Singh, Fahad Mansoor Samadi, Shaleen Chandra, Roop Ganguly, and Shaista Suhail. "Evaluation of hematological parameters in oral cancer and oral pre-cancer." International Journal of Basic & Clinical Pharmacology 9, no. 7 (2020): 1090. http://dx.doi.org/10.18203/2319-2003.ijbcp20202947.
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Background: Oral squamous cell carcinoma (OSCC) is the most common public health issue in Indian population. Quite a large number of OSCC cases are preceded by potentially malignant disorders of oral cavity. The need for simple diagnostic marker for early diagnosis and thus better therapeutic outcome is imperative. The current study aims to evaluate hematological parameters such as hemoglobin, bleeding time, clotting time, total leucocyte count (TLC) and differential leucocyte count (DLC) in OSCC and oral potentially malignant disorder cases along with normal healthy controls.Methods: A total of 150 subjects; 50 in each group were taken and 2.5 ml of blood were withdrawn from each subject and TLC, DLC and hemoglobin assessment was done using autoanalyzer while bleeding time and clotting time was recorded through Duke’s method and modified Dale’s method respectively.Results: All the data were tabulated and recorded as mean±standard deviation and comparison was done using one-way ANOVA test (p<0.05). TLC count, neutrophil count and lymphocyte count showed statistically significant difference amongst three groups while other parameters such as hemoglobin percentage, bleeding time, clotting time, eosinophil count, monocyte count and basophil count were statistically non-significant.Conclusions: This study showed TLC count, neutrophil count and lymphocyte count might prove as useful determinant factor in oral squamous cell carcinoma and oral potentially malignant disorders. However, further study with larger sample size is required to establish their role as diagnostic, prognostic marker or predictor of malignant transformation.
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N, Malathi, Rino T, Jaya Supa Sooriya J, and Shalini M. "COMPARATIVE STUDY OF HAEMATOLOGICAL PARAMETERS IN FISHES FROM THE NATURAL HABITAT, MADURAI REGION." Journal of Experimental Biology and Agricultural Sciences 9, Spl-1- GCSGD_2020 (2021): S176—S181. http://dx.doi.org/10.18006/2021.9(spl-1-gcsgd_2020).s176.s181.
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The heamatological profile of two freshwater fishes Labeo rohita (Rohu) and Channa striatus (Murrel) with a relationship to the sex and the water quality under natural habitat was analyzed. The freshwater fishes were collected from the pond located in Thodaneri, Vadipatti taluk, Madurai district, Tamil Nadu, India. In heamatology studies, no significant variation was observed in Heamoglobin content and found almost the same in both the fish irrespective of the sex and also falls in the range of control value. In differential Leucocyte count (DLC) the following blood components Lymphocyte, Eosinophil, and Monocyte count were significantly higher than the control values and the values are found maximum in Rohu when compared to Murrel.RBC (Erythrocyte) count was almost the same in both the fish and the value falls between the range of control value Packed Cell Volume (PCV) count was almost the same in both the fish and the value falls between the range of control value. The values of Hb and PCV were found higher in male fish as compared to female fish. The leucocyte count is also higher in the males when compared to the females of both Rohu and Murrel. These may be also influenced by physiological activity, feeding habits, infectious agents, water quality parameters, and the environmental stress on the organism.
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Amidu, Nafiu, Benedict Boateng Antuamwine, Martin Awe Akilla, et al. "Leucocyte Differential Count and Pregnancy Induced Hypertension: Implication for Risk and Disease Assessment." American Journal of Laboratory Medicine 5, no. 1 (2020): 32. http://dx.doi.org/10.11648/j.ajlm.20200501.15.
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Iqbal, Mohammad Faruq, Arif Imtiyaz Chowdhury, and Sharkar Rushdi Aziz. "Role of C-reactive Protein, Leucocyte and Neutrophil Count in Diagnosis of Acute Appendicitis." Journal of Armed Forces Medical College, Bangladesh 17, no. 1 (2022): 26–29. http://dx.doi.org/10.3329/jafmc.v17i1.56717.
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Introduction: Acute appendicitis is the most common cause of surgical acute abdomen and appendicectomy is the most commonly done surgery. Accurate preoperative diagnosis can reduce negative appendicectomy rate. Objective: To evaluate the diagnostic accuracy of C-reactive protein (CRP), total leucocyte count (TLC) and neutrophil count in patients operated after clinical diagnosis of acute appendicitis. Materials and Methods: This cross-sectional observational study was conducted among 100 patients at Combined Military Hospital, Dhaka from August 2012 to January 2013. Serum CRP level was measured by latex agglutination method which is a qualitative test and level of 6 mg / L was considered positive. TLC and neutrophil count were estimated by processing blood sample through automated haematology analyzer. TLC level of 10.5 x 109/L and differential count of neutrophil more than 75% were considered elevated. Results: Out of 100 cases the mean age of the patients was 21.7 years and 65 patients were male and 35 patients were female with male to female ratio 1.85:1. Majority cases (54%) were in the age group of 11-20 years. Clinical diagnosis was correct in 93% cases and negative appendicectomy rate was 07% (p<0.001). Appendix was found mostly inflamed (83%) and gangrenous (10%). CRP was highly sensitive (84.9%) and specific (85.7%) with diagnostic accuracy of 85%. TLC was also found equally sensitive and specific with diagnostic accuracy of 83%. Overall diagnostic accuracy of neutrophil was 76%. Conclusion: Diagnosis of acute appendicitis is essentially clinical supported by simple laboratory investigations like TLC and neutrophill count. Elevated levels of CRP, TLC and neutrophill count are helpful in confirming a suspected diagnosis of acute appendicitis. Appendicitis is very unlikely if the CRP, TLC and neutrophill counts are normal. JAFMC Bangladesh. Vol 17, No 1 (June) 2021: 26-29
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Igbokwe, N. A., I. J. Omeh, and G. Kashim. "Beebread and Honey improves some Haematologic Parameters in Male Albino Mice." Sahel Journal of Veterinary Sciences 19, no. 3 (2022): 37–41. http://dx.doi.org/10.54058/saheljvs.v19i3.252.
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The effects of honey and beebread on some haematological parameters were investigated in fifteen apparently healthy adult albino male mice. The mice were divided into three groups of five mice each after a two-week period of acclimatization. The mice in group A were given distilled water orally to serve as a control while those in groups B and C were administered honey and beebread respectively by gavages at the dose rate of 1g/kg body weight and at a concentration of 40% w/v using water as a vehicle, once daily for 70 consecutive days. Micro-haematocrit and hematocytometry were used for determination of packed cell volume and erythrocyte and differential leucocyte counts, respectively. Haemoglobin concentration was estimated by using Cyanmethemoglobin method. There were significant (p ˂0.05) increases in the red blood cells count, packed cell volume and haemoglobin concentration in the treatment groups compared to the control group, with the most increase observed in the group treated with beebread. There was significant (p ˂ 0.05) increase in total leucocyte count in the treated groups with the highest count recorded in the honey treated group than the control. Lymphocytes and neutrophils significantly (p ˂ 0.05) increased in the treatment groups but there were no significant (P > 0.05) changes in the number of eosinophils, basophils and monocytes. The study demonstrates the positive haematopoietic effects of supplementation of feed with honey and beebread.
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Erişmiş, Uğur, Hüseyin Arikan, Nurşen Alpagut-Keskin, and İ. Ethem Çevik. "A study on the blood cells of the fire-bellied toad, Bombina bombina L. (Anura: Bombinatoridae)." Animal Biology 60, no. 1 (2010): 61–68. http://dx.doi.org/10.1163/157075610x12610595764174.
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AbstractThe aim of this study was to determine the blood cells parameters of Anatolian and Thracian Bombina bombina populations. The size and counts of various blood cells were analyzed in 29 mature Bombina bombina specimens belonging to two populations (Durusu-Istanbul and Döşeme Bataklığı–Adapazarı) in northwest Anatolia. The mean erythrocyte count was determined as 251.429/mm3, that of leucocytes was 2.114/mm3, and that of thrombocytes was 12.857/mm3. There was no significant sexual variation in blood cell counts of our B. bombina sample which was collected during their breeding season. The leucocyte differential counts and staining properties of blood cells were also determined. Interspecific comparisons indicate that northwest Anatolian B. bombina samples share general features with other anurans but differ from other populations of the same species in blood cell parameters. The lower number of erythrocyte and their relatively large surface area of our samples suggest a lower level of metabolism and relatively higher oxygen uptake. We suggest that this structure is probably not only due to adaptations to different geographic regions and altitude but also a combination of recent habitat reductions due to human actions and agricultural contaminants may account for the blood cell parameters as well as other genetic and morphologic patterns of these isolated and small populations of this species.
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Chaturvedi, Shweta, Gajendra Nath Gupta, Mansi Faujdar, Rohit Jain, and Rateesh Sareen. "Body fluid analysis by 'Automated' Sysmex XN 1000 – The way ahead." International Journal of Clinicopathological Correlation 8, no. 1 (2024): 22–28. http://dx.doi.org/10.56501/intjclinicopatholcorrel.v8i1.1075.
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Body fluids are those excreted or secreted from the body. In the laboratory, the use of the term “body fluids” designates a category that excludes blood and urine. Determining the etiologic cause of fluid accumulation in various body cavities (i.e., joints, chest, and abdomen) is critical for proper treatment of various disorders. To evaluate the correlation and agreement of leucocyte and erythrocyte count in body fluids among Improved Neubauer Chamber and automated method using Sysmex XN-1000 body fluid module. A total of 500 routinely collected body fluid samples (CSF, ascitic fluid, pericardial fluid and pleural fluid) were included in the study. Total WBC counts were performed manually using improved Neubauer chamber and microscopic differential counts were performed using Leishman stained slides. Corresponding results of Sysmex XN-1000 body fluid module analysis were matched to these particular count categories. Absolute WBC cell counts showed a high correlation and agreement between methods (r> 0.9, p value=<0.001). However, a fair correlation existed between the lymphocyte percentage and neutrophil percentage measured through the two methods (r= 0.436 and r=0.447 respectively, p < 0.001). Body fluid module application of Sysmex XN-1000 serves as an acceptable alternative to manual method for measuring the total nucleated cell counts and for total RBC count. However, it cannot be recommended as a suitable alternative for manual differential cytologic workup. Keywords: Sysmex XN 1000, Body fluid analysis, Cerebrospinal fluid analysis, Improved Neubauer Chamber
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Tenório, Thiago Ricardo dos Santos, Breno Quintella Farah, Raphael Mendes Ritti-Dias, et al. "Relation between leukocyte count, adiposity, and cardiorespiratory fitness in pubertal adolescents." Einstein (São Paulo) 12, no. 4 (2014): 420–24. http://dx.doi.org/10.1590/s1679-45082014ao3214.
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Objective To compare the total and differential leukocyte count in obese and normal-weight adolescents, and to verify their possible relations with cardiorespiratory fitness and adiposity indicators.Methods A cross-sectional study conducted with 139 adolescents (107 obese and 32 normal weight) aged between 13 and 18 years. Cardiorespiratory fitness was determined by direct gas analysis during an incremental treadmill test. Total leukocytes and subsets were estimated by flow cytometry. Body composition was assessed by dual-energy X-ray absorptiometry. Thet-test for independent samples was used for comparison between groups. The relation between leukocytes, cardiorespiratory fitness and adiposity indicators was verified by Pearson’s correlation and multiple linear regression (adjusted for age and body mass index) tests.Results Obese adolescents had higher leukocyte (8.12±2.36u/L x 103; p=0.001), neutrophil (4.33±1.86u/L x 103; p=0.002), and monocyte (0.70±0.22u/L x 103; p=0.002) counts compared to the levels of normal weight subjects. After the necessary adjustments, cardiorespiratory fitness had a negative association with leukocytes, neutrophils, and monocytes in boys.Conclusion Obese adolescents had higher total and differential leucocyte count when compared to normal weight individuals. We also observed a weak positive association between adiposity and total leukocyte, monocyte, and neutrophil counts, and in boys, a negative association between cardiorespiratory fitness and total count of leukocytes, monocytes, and neutrophils.
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Akshay, Louis Dias, and D. Sumanth. "Usefulness of Various Peripheral Blood Leukocyte Count ratios in Malaria Evaluation." International Journal of Health Sciences and Pharmacy (IJHSP) 1, no. 2 (2017): 52–60. https://doi.org/10.5281/zenodo.1043523.
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Malaria is a major public health problem in India. Changes in blood cell counts are a well-known feature of malaria. Hence an attempt has been made to assess the role of leucocyte ratios in malaria as predictors of malaria infection and its clinical severity. <strong>Methods:</strong> A retrospective observational cohort study was carried out in a tertiary care teaching hospital over a period of 2 months. A total of 171 samples and 48 controls were included in the study. All the absolute and differential white blood cell counts were done. The neutrophil-lymphocyte count ratio (NLCR), monocyte-lymphocyte count ratio (MLCR) and monocyte-neutrophil count ratio (MNCR) were calculated by using the absolute neutrophil, lymphocyte and monocyte count respectively. The results were analyzed statistically using SPSS software for windows. <strong>Results:</strong> Among 171 cases 145(84.8%), 19(11.1%), 7(4.1%) were Pl. vivax, Pl. falciparum and mixed malaria respectively. 52 cases were severe and 119 were non-severe. 52, 45, 47, 27 were parasitemia grade 1, 2, 3, 4 respectively. NLCR, MLCR and MNCR showed a sensitivity of 60.8%, 71.7%, 70.8% and specificity of 43.8%, 60.4%, and 56.3% respectively in malaria diagnosis. There was no association of NLCR, MLCR, MNCR with the type and severity of malaria. NLCR, MNCR correlated with varying grade of parasitemia. <strong>Conclusion:</strong> The results show the ratios NLCR, MLCR and MNCR have a better sensitivity but less specificity. The sensitivity and specificity for MLCR are the highest and can be used as a supportive to a screening test. The ratios can be used to show the presence of malaria parasite but there is no association with the type and severity of malaria. MLCR is a good predictor of malarial presence. NLCR and MNCR are associated with parasitemia.
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Ememe, M. U. "Seasonal Variations of Haematological and Biochemical Parameters Associated with Potential Laminitis in Apparently Healthy Horses." Journal of Veterinary and Biomedical Sciences 4, no. 1 (2022): 30–36. http://dx.doi.org/10.36108/jvbs/2202.40.0140.
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Early detection of laminitis in horses is valuable as it provides information that can be used as a basis for advice to trainers of race and performance horses. The study was aimed to determine the seasonal variations in haematological and biochemical parameters of horses developing laminitis at Polo Club Port Harcourt, River State, Nigeria. A total of 18 horses were sampled during the dry season (February) and rainy season (July). The horses sampled were aged between 3 and 8 years. The hoof temperature, weight, packed cell volume (PCV), red blood cell (RBC) count, hemoglobin (Hb) concentration, total and differential leucocyte counts, glucose level and fibrinogen concentration were determined using standard methods. The mean hoof temperature of the horses was 36.1± 0.11oC and 35.6 ± 0.2 oC during the dry and rainy season respectively. The mean body weight of the horses was 323 ± 8.90 kg in dry season while that of rainy season was 330 ± 11.0 kg. Glucose concentration during the dry season (71.6 ± 1.5 dl/mg) was significantly lower (P ˂ 0.05) than that obtained during the rainy season (91.7 ± 1.9 dl/mg). Furthermore, the PCV, RBC count and Hb concentration were significantly higher (P ˂ 0.05) in the rainy season than the corresponding parameters in the dry season. Similarly, the total leucocyte, neutrophil and lymphocyte counts were significantly higher (P ˂ 0.05) during the rainy season than the dry season. It was concluded that variations on the parameters determined may aid to identify early warning signs of laminitis.
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Gupta, Tavish, and Debdatta Basu. "Utility of scatterplot patterns of automated hematology analysers in white blood cell disorders – A comparative study with peripheral blood smears." Asian Journal of Medical Sciences 11, no. 5 (2020): 29–34. http://dx.doi.org/10.3126/ajms.v11i5.29049.
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Background: Advances in automated blood cell counting offer new possibilities to analyse leukocyte differentials by fluorescence RNA/DNA staining combined with scattered light intensity technologies.
 Aims and Objectives: The purpose of this study is to identify the scatterplot patterns in normal cell counts and various White Blood Cell (WBC) disorders and to assess their efficacy compared to a peripheral blood smear (PBS) in the diagnosis of WBC disorders.
 Materials and Methods: A three-phased descriptive study analysing a total of 933 EDTA blood samples. The total leucocyte count, differential leucocyte count, scatterplot patterns as obtained by automated cell counter and PBS were studied in 405 normal samples (Phase 1) and 415 samples with WBC abnormalities (Phase 2). In phase 3, the scatterplots of 113 abnormal flagged samples were analysed and provisional diagnosis made in each case which was then compared with the diagnosis on the PBS. Sensitivity, Specificity, Positive and Negative predictive values (PPV & NPV) were calculated.
 Results: A grid-based standard scatterplot of normal samples was generated as a reference plot in phase one. Based on the scatterplots obtained in known WBC abnormalities studied in phase two, the basic abnormal patterns specific to each of the individual abnormalities were discerned. The phase three study showed excellent correlation between scatterplots and PBS findings in neutrophilia, eosinophilia, lymphocytosis and leukopenia (sensitivity 92-100%, specificity 98-100%). Abnormalities suspected to be leukemia on the scatterplots had a relatively low sensitivity and PPV.
 Conclusion: WBC scatterplots can be used as a screening tool as it correlates well with various WBC disorders in the PBS.
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Piccione, G., M. Rizzo, F. Arfuso, et al. "Leukocyte modifications during the first month after foaling in mares and their newborn foals." Polish Journal of Veterinary Sciences 18, no. 3 (2015): 621–25. http://dx.doi.org/10.1515/pjvs-2015-0080.
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Abstract During early post-partum period both neonatal foals and peripartum mares are most susceptible to diseases. The aim of this study was to establish physiologic modifications of leukogram during the first month after foaling in mares and their newborn foals. To this end blood samples were collected from nine mares and nine foals (T0-T10), every three days from the 1st day until the 30th day after foaling. Samples were analysed for white blood cell (WBC) count and differential leucocyte counts. Two-way repeated measure analysis of variance (ANOVA) showed, in postpartum mares WBC showed significant higher values at T0 (9.02±0.76) in respect to other time points, and at T2 (8.08±0.53) and T3 (7.92±0.59) compared to T1 (6.98±0.43), whereas in foals lower WBC values at T0 (6.11±0.49) compared to other experimental periods except T1 (6.90±0.94), and at T1 compared to T8 (7.95±0.61) and T10 (7.90±0.36) were observed. The differential leucocyte counts showed significant modifications in the percentage of neutrophils (π<0.001) and lymphocytes (p<0.001) both in postpartum mares and in foals during the experimental period. Furthermore ANOVA showed significant differences between postpartum mares and foals (P<0.01) in all studied parameters, and between postpartum mares and control mares in WBC and neutrophils values. The obtained results provide suitable information about the influence of foaling on leukogram of periparturient mares and reveal WBC dynamics in newborn foals during the first month post-partum.
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Choudhary, Pankaj K., Ajay K. Ishwar, Pramod Kumar, et al. "Effect of Exogenous Melatonin and Different Photoperiods on Differential Leucocyte Count in Chhotanagpuri Ewe." Indian Journal of Veterinary Sciences and Biotechnology 17, no. 02 (2007): 30–34. http://dx.doi.org/10.21887/ijvsbt.17.2.6.
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Forty-two healthy, non-pregnant, non-lactating Chhotanagpuri ewe, having body weight ranging from 14.11 ± 0.09 to 15.38 ± 0.06 kg, were selected and isolated from rams 2 months before melatonin administration. The selected animals were allocated randomly into seven groups each of 6 animals, viz., Group-I Normal control, Group-II Long day (LD) control), Group-III (LD + melatonin administration 3 mg/day orally, Group-IV
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BARTELS, P. C. M., M. SCHOORL, and F. L. A. WILLEKENS. "Evaluation of Sysmex SF-3000 performance concerning interpretive morphology flagging ofthe leucocyte differential count." Clinical and Laboratory Haematology 19, no. 3 (1997): 187–90. http://dx.doi.org/10.1046/j.1365-2257.1997.00075.x.
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NDIDI UGWU, GLADYS, and CHIMAOBI ODINAKA UGORJI. "PHYTOCONSTITUENTS, ACUTE TOXICITY AND THE EFFECT OF METHANOL EXTRACT OF Azanza garckeana (MALVACEAE) ON THE HAEMATOLOGICAL INDICES OF ADULT WISTAR RATS." AFRICAN JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT 16, no. 3 (2024): 117–29. https://doi.org/10.59493/ajopred/2024.3.13.
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Azanza garckeana is a tropical shrub and fruit-bearing tree. The plant belongs to the Malvaceae family (F. Hoffm) Exell & Hillc and a semi-decidious flowering plant that grows in Savannah-wooded grasslands and open forests. All parts of the plant are useful for both medical and economic purposes. One (1) kg of the powdered material was used and the methanol crude extract of the plant material was obtained by the Soxhlet extraction process. This study aimed to determine the effect of methanol extract of A. garckeana on different haematological indices were also explored which include red blood cells, White blood cells (WBC), and differential leucocyte count (DLC). Adult albino rats were divided into five (5) groups of five each. Groups 1 to 3 received 100 mg/kg, 200 mg/kg and 400 mg/kg of Azanza garckeana respectively. Group 4 received 5 mL/kg distilled water and served as the normal control while Group 5 received 2.5 mL/kg of Astyfer. The different doses of the methanol extract were administered to the animals orally for three weeks. On day 0 and 21, blood samples were collected and subjected to haematological tests. At the end of this study, it was observed that the extract increased the levels of haemoglobin and red blood cells without showing a significant increase in the total WBC count, and differential leucocyte count. The extract’s phytochemical study revealed the presence of many phytochemical elements, including reducing sugar, terpenoids, flavonoids, alkaloids, phenols, saponins, and tannins. No alkaloid was found. The plant is not fatal, according to the acute toxicity assessment, and the extract is deemed to be rather harmless. Flavonoids and tannins were responsible for the methanol extract’s haematological characteristics. When compared to the negative and positive control groups, the methanol extract did not result in a statistically significant (p<0.05) rise in the albino rats’ PCV, WBC, lymphocytes, monocytes, basophils, and neutrophil counts at the dose levels examined. The myth surrounding the usage of the herb to cure low blood level conditions is dispelled by this study.
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Berebon, Dinebari, Chimaobi Ugorji, Chigoziri F. Nwanaforo, et al. "Phytonutrients, Acute Toxicity and the Effect of Dichloromethane-Methanol Extract of Cymbopogon citratus (DC. ex Nees) Stapf (Poaceae) on Selected Haematological Parameters in Normal Healthy Albino Rats." Archives of Ecotoxicology 5, no. 2 (2023): 52–60. http://dx.doi.org/10.36547/ae.2023.5.2.52-60.
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This study aimed to evaluate the effect of the Dichloromethane-Methanol (DCM-MeOH) extract of Cymbopogon citratus on selected haematological parameters and to identify the phytochemical components present in the extract. The (DCM-MeOH) extract of the plant was obtained by 72 h cold maceration and was subjected to haematological screening. Four groups of rats were used; group 1 served as normal control which received distilled water, groups 2, 3, and 4 received daily administration of (DCM-MeOH) extract of C. citratus (100, 200 and 400 mg/kg) for 21 days per oral. Haematological analyses were performed weekly for the red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), and haemoglobin concentration (HB) with an absolute count of differential leucocyte were determined. The acute toxicity study on the extract did not produce any lethality up to 5000 mg/kg in mice. The result of the study showed a significant (P < 0.05) effect to varying degrees in the weight and haemoglobin while the RBC, PCV, differential leucocyte count (DLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) did not have significant (P > 0.05) effect as compared with the normal control group and baseline values. The qualitative phytochemical screening showed the presence of flavonoids, terpenoids, alkaloids, steroids and reducing sugars while protein, carbohydrate, fat and oil, glycosides and saponins were absent in the extract. However, quantitative phytochemical studies showed that the extract contained alkaloids (465.83 mg/100g), flavonoids (119.00 mg/100g), tannins (16.25 mg/100g), terpenoids (203.36), total phenols (1897.58). The findings from this study showed that DCM-MeOH extract of C. citratus does not have any significant effect on most haematological parameters in normal healthy albino rats.
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Ling, Ying, Mary Jane Bell, Lisa Chodirker, and Shirley Lake. "Missed hypereosinophilic syndrome in a critically ill patient with systemic lupus erythematosus." BMJ Case Reports 14, no. 1 (2021): e236592. http://dx.doi.org/10.1136/bcr-2020-236592.
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A high functioning 74-year-old man with systemic lupus erythematosus presented to the emergency department with acute anxiety. He was found to have elevated cardiac enzymes and admitted to the cardiology service for investigation. In hospital, he developed an erythematous papular rash, and deteriorated to being somnolent and bedridden. He was found to have new multiterritory ischaemic strokes. It was eventually noted that he had persistent eosinophilia, present even on admission, which had been overlooked as the total leucocyte count was normal. Serology for antiphospholipid antibody syndrome (APS) was positive. He was diagnosed with hypereosinophilic syndrome (HES) secondary to new APS, and responded to high-dose steroids. This case highlights the importance of fully evaluating a leucocyte differential to make a diagnosis of HES. We discuss the definition, clinical manifestations, diagnostic approach and management of this important condition.
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Sajid, M., M. Kashif, A. Rasool, Muhammad Umer Farooq, and W. Nazar. "Detection of Selected Heavy Metals and Their Effect on Haematological and Serum Biochemical Values in Naturally Exposed Cattle." Journal of Agricultural Sciences – Sri Lanka 20, no. 2 (2025): 182–87. https://doi.org/10.4038/jas.v20i2.9951.
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Purpose: The present study was conducted in two areas (one clean and one contaminated) of the district of Jhang, Pakistan, to determine the concentration of the possible heavy metals, lead and cadmium, in the blood. For this purpose, two hundred samples were collected, one hundred from each area.Research Method: These samples were analyzed for lead and cadmium using the atomic absorption spectrophotometric method. The haematological parameters packed cell volume, total erythrocyte count, Haemoglobin, total leucocyte count, differential leukocyte count, and erythrocyte sedimentation rate were measured to evaluate the effect of heavy metals on these parameters. A serological study was also conducted to observe its effects on alanine transaminase, aspartate transaminase, urea, and creatinine.Findings: The results showed that the mean values of lead were significantly higher (P<0.05) in contaminated areas compared to clean areas, while the cadmium level was below the detection limit. The mean values of red blood cells, total leucocyte count, Haemoglobin, packed cell volume, and erythrocyte sedimentation rate showed statistically non-significant differences. The mean values of alanine transaminase were statistically higher in contaminated areas as compared to clean areas, whereas the levels of aspartate transaminase, urea, and creatinine showed non-significant differences in both areas. The presence of lead above permissible levels is alarming from one health point of view, so there is a dire need for legislation to control this transmission of heavy metals to humans and animals.
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BARTELS, P. C. M., M. SCHOORL, and F. L. A. WILLEKENS. "Evaluation of Sysmex SF-3000 performance concerning interpretive morphology flagging of the leucocyte differential count." Clinical & Laboratory Haematology 19, no. 3 (1997): 187–90. http://dx.doi.org/10.1111/j.1365-2257.1997.tb00005.x.
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Nukala, Suresh, Fnu Aparna, Mariha Feliciano, and Jessica Lynn Withey. "Thrombocytopenia, renal failure and hearing loss in a young patient:MYH9-related disorder." BMJ Case Reports 18, no. 6 (2025): e262915. https://doi.org/10.1136/bcr-2024-262915.
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A young woman with a history of thrombocytopenia was treated for idiopathic thrombocytopenic purpura (ITP) with splenectomy, intravenous immunoglobulin, steroids and chemotherapeutic agents. The patient experienced hearing loss during childhood and, as a teenager, was diagnosed with hypertension and nephrotic-range proteinuria, which progressed to renal failure requiring dialysis. On presentation to our institution, her platelet count was 13×109 /L. Peripheral blood smear showed giant platelets and leucocyte inclusions. The presence of thrombocytopenia with giant platelets, leucocyte inclusions, renal failure and hearing loss raised the possibility ofMYH9-related disorder (MYH9-RD). Genetic testing revealed a pathogenic mutation, c.2105G>A (p.Arg702His), confirming the diagnosis. Patients withMYH9-RD are frequently misdiagnosed with ITP and consequently receive futile and potentially toxic interventions. ConsideringMYH9-RD as a differential diagnosis in patients presenting with thrombocytopenia is important, especially when there is a history of early-onset renal failure, hearing loss, or cataracts.
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Akhtar, Suraiya, Susane Giti, Md Shahidul Islam, and Sharif Mohammad Rezaul Masud. "Haematological Profile and its Clinical Implications in Dengue Viral Infections." Journal of Armed Forces Medical College, Bangladesh 19, no. 1 (2024): 11–14. http://dx.doi.org/10.3329/jafmc.v19i1.68295.
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Introduction: Dengue fever is the most common arboviral disease in the world. Transmission to humans of dengue virus initiates a spectrum of illness, from inapparent to severe and sometimes lethal infections. Complete blood count (CBC) is an important part of diagnostic workup of patients. Comparison of various findings in CBC and peripheral blood film can help the physician in diagnosis, management and assessment of prognosis of the patients. Objectives: To find out the pattern of haematological findings in dengue infection. Methods: This cross sectional study was carried out on 62 dengue cases in CMH Savar from May to October 2019. Diagnosis of dengue was based on clinical features, haematological and biochemical findings and confirmed by serological tests. Haematological parameters analyzed include haemoglobin (Hb), haematocrit, total leucocyte count (TLC), differential leucocyte count (DLC), platelet count and peripheral blood film. Results: Important findings in haematological profile were haemoconcentration, raised haematocrit, low WBC count, early neutrophilia and late lymphocytosis, reactive lymphocytes in peripheral blood and thrombocytopenia. Haemoglobin ranged from 10.0-18.5gm/dl. Haematocrit was raised in 13(20.96%) cases. Thrombocytopenia (platelet count <1.5 Lac/mm3) was observed in 59(95.16%) cases. Platelet count 1-1.5 Lac was seen in 4(6.45%) cases, 50,000-1 Lac in 25(40.32%) cases, 20,000-50,000 in 26(41.93%) cases and < 20,000 in 4(6.45%) cases. Leucopenia (TLC<4000/mm3) was seen in 46(74.19%) cases and >11000mm3 in 2(3.22%) cases. On peripheral blood smear reactive lymphocytes including plasmacytoid lymphocytes were seen in almost all the cases. Conclusion: CBC examination gives enough clue for early diagnosis of dengue infection which can be confirmed by dengue serology. Serial CBC examination is also important in disease monitoring, treatment of the patient and prediction of prognosis. JAFMC Bangladesh. Vol 19, No 1 (June) 2023:11-14