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Journal articles on the topic 'Diffusion ATP'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 May 2022

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1

Vendelin, Marko, and Rikke Birkedal. "Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy." American Journal of Physiology-Cell Physiology 295, no. 5 (2008): C1302—C1315. http://dx.doi.org/10.1152/ajpcell.00313.2008.

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A series of experimental data points to the existence of profound diffusion restrictions of ADP/ATP in rat cardiomyocytes. This assumption is required to explain the measurements of kinetics of respiration, sarcoplasmic reticulum loading with calcium, and kinetics of ATP-sensitive potassium channels. To be able to analyze and estimate the role of intracellular diffusion restrictions on bioenergetics, the intracellular diffusion coefficients of metabolites have to be determined. The aim of this work was to develop a practical method for determining diffusion coefficients in anisotropic medium a

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2

Song, Zhiyan, Hua Zhao, Olarongbe Olubajo, Lewis B. Hall, Chauncey N. Orr, and Courtney B. Askew. "Characterizing the binding of nucleotide ATP on serum albumin by 31P NMR diffusion." Canadian Journal of Chemistry 90, no. 5 (2012): 411–18. http://dx.doi.org/10.1139/v2012-011.

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The pulsed-field-gradient (PFG) 31P NMR diffusion spectra were measured under varied sample conditions to characterize the low-affinity binding of adenosine 5′-triphosphate (ATP) on human serum albumin (HSA) or bovine serum albumin (BSA). The NMR diffusion constants of ATP, ATP–HSA, or ATP–BSA were illustrated as function of ATP concentrations. The binding curves of ATP–HSA and ATP–BSA were identical but strikingly different from the ATP curve. Using a “Scatchard plot”, the apparent binding constant (K) and number of ATP binding sites (n) on serum albumin were evaluated as K = 75.25 (mol/L)–1

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3

Jones, D. P. "Intracellular diffusion gradients of O2 and ATP." American Journal of Physiology-Cell Physiology 250, no. 5 (1986): C663—C675. http://dx.doi.org/10.1152/ajpcell.1986.250.5.c663.

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Endogenous enzymes with different subcellular localizations provide in situ probes to study O2 and ATP concentration at various sites within cells. Results from this approach indicate that substantial intracellular concentration gradients occur under some O2- and ATP-limited conditions. These studies, along with electron microscopic analyses and mathematical modeling, indicate that clustering and distribution of mitochondria are major factors in determining the magnitude and location of the concentration gradients. The mitochondria appear to be clustered in sites of high ATP demand to maximize

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4

Wohl and Sherman. "ATP-Dependent Diffusion Entropy and Homogeneity in Living Cells." Entropy 21, no. 10 (2019): 962. http://dx.doi.org/10.3390/e21100962.

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Intracellular dynamics is highly complex, and includes diffusion of poly-dispersed objects in a non-homogeneous, out-of-equilibrium medium. Assuming non-equilibrium steady-state, we developed a framework that relates non-equilibrium fluctuations to diffusion, and generalized entropy in cells. We employed imaging of live Jurkat T cells, and showed that active cells have higher diffusion parameters (Kα and α) and entropy relative to the same cells after ATP depletion. Kα and α were related in ATP-depleted cells while this relation was not apparent in untreated cells, probably due to non-equilibr

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5

Gupte, Sharmila Shaila, Brad Chazotte, Michael A. Leesnitzer, and Charles R. Hackenbrock. "Two-dimensional diffusion of F1F0-ATP synthase and ADP/ATP translocator. Testing a hypothesis for ATP synthesis in the mitochondrial inner membrane." Biochimica et Biophysica Acta (BBA) - Biomembranes 1069, no. 2 (1991): 131–38. http://dx.doi.org/10.1016/0005-2736(91)90114-n.

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6

Koh, H. R., M. A. Kidwell, K. Ragunathan, J. A. Doudna, and S. Myong. "ATP-independent diffusion of double-stranded RNA binding proteins." Proceedings of the National Academy of Sciences 110, no. 1 (2012): 151–56. http://dx.doi.org/10.1073/pnas.1212917110.

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7

Handly, L. Naomi, and Roy Wollman. "Wound-induced Ca2+ wave propagates through a simple release and diffusion mechanism." Molecular Biology of the Cell 28, no. 11 (2017): 1457–66. http://dx.doi.org/10.1091/mbc.e16-10-0695.

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Damage-associated molecular patterns (DAMPs) are critical mediators of information concerning tissue damage from damaged cells to neighboring healthy cells. ATP acts as an effective DAMP when released into extracellular space from damaged cells. Extracellular ATP receptors monitor tissue damage and activate a Ca2+ wave in the surrounding healthy cells. How the Ca2+ wave propagates through cells after a wound is unclear. Ca2+ wave activation can occur extracellularly via external receptors or intracellularly through GAP junctions. Three potential mechanisms to propagate the Ca2+ wave are source

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8

Ramm, Beatrice, Andriy Goychuk, Alena Khmelinskaia, et al. "A diffusiophoretic mechanism for ATP-driven transport without motor proteins." Nature Physics 17, no. 7 (2021): 850–58. http://dx.doi.org/10.1038/s41567-021-01213-3.

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AbstractThe healthy growth and maintenance of a biological system depends on the precise spatial organization of molecules within the cell through the dissipation of energy. Reaction–diffusion mechanisms can facilitate this organization, as can directional cargo transport orchestrated by motor proteins, by relying on specific protein interactions. However, transport of material through the cell can also be achieved by active processes based on non-specific, purely physical mechanisms, a phenomenon that remains poorly explored. Here, using a combined experimental and theoretical approach, we di

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9

Cho, Won-Ki, Cherlhyun Jeong, Daehyung Kim, et al. "ATP Alters the Diffusion Mechanics of MutS on Mismatched DNA." Structure 20, no. 7 (2012): 1264–74. http://dx.doi.org/10.1016/j.str.2012.04.017.

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10

Horvath, Laszlo I., Anton Munding, Klaus Beyer, Martin Klingenberg, and Derek Marsh. "Rotational diffusion of mitochondrial ADP/ATP carrier studied by saturation-transfer electron spin resonance." Biochemistry 28, no. 1 (1989): 407–14. http://dx.doi.org/10.1021/bi00427a056.

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11

Hummerich, H., H. de Groot, T. Noll, and S. Soboll. "Dependence of mitochondrial and cytosolic adenine nucleotides on oxygen partial pressure in isolated hepatocytes. Application of a new rapid high pressure filtration technique for fractionation." Biochemical Journal 250, no. 3 (1988): 641–45. http://dx.doi.org/10.1042/bj2500641.

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By using a new rapid high pressure filtration technique, mitochondrial and cytosolic ATP and ADP contents were determined in isolated hepatocytes at different oxygen partial pressures. At 670 mmHg, subcellular adenine nucleotide contents and ATP/ADP ratios were comparable with values obtained with the digitonin fractionation technique. However at lower oxygen partial pressure ADP appears to be rephosphorylated during digitonin fractionation whereas with high pressure filtration fractionation rephosphorylation of ADP is avoided due to shorter fractionation times. Cytosolic and mitochondrial ATP

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12

Soga, Naoki, Kazuya Kimura, Kazuhiko Kinosita, Masasuke Yoshida, and Toshiharu Suzuki. "Perfect chemomechanical coupling of FoF1-ATP synthase." Proceedings of the National Academy of Sciences 114, no. 19 (2017): 4960–65. http://dx.doi.org/10.1073/pnas.1700801114.

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FoF1-ATP synthase (FoF1) couples H+ flow in Fo domain and ATP synthesis/hydrolysis in F1 domain through rotation of the central rotor shaft, and the H+/ATP ratio is crucial to understand the coupling mechanism and energy yield in cells. Although H+/ATP ratio of the perfectly coupling enzyme can be predicted from the copy number of catalytic β subunits and that of H+ binding c subunits as c/β, the actual H+/ATP ratio can vary depending on coupling efficiency. Here, we report actual H+/ATP ratio of thermophilic Bacillus FoF1, whose c/β is 10/3. Proteoliposomes reconstituted with the FoF1 were en

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13

OHSUGI, Y., and MASATAKA KINJO. "ANALYSIS OF MEMBRANE-BINDING PROTEIN MOBILITY IN LIVING CELLS USING TOTAL INTERNAL REFLECTION FLUORESCENCE CORRELATION SPECTROSCOPY." Biophysical Reviews and Letters 01, no. 03 (2006): 293–99. http://dx.doi.org/10.1142/s1793048006000227.

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Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) is an appropriate method for measuring diffusion constants and the number of fluorescent molecules very close to the coverglass surface. Recently, we have reported the application of TIR-FCS to cell biology, measuring membrane-binding farnesylated green fluorescent proteins (EGFP-F) in living cells. In this research, we measured the signal transduction molecule, protein kinase C (PKC), fused with EGFP in living HeLa cells by using TIR-FCS. We observed two different diffusional mobilities of PKCβII-EGFP, three-dimensional

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14

van Beek, Johannes H. G. M. "Adenine nucleotide-creatine-phosphate module in myocardial metabolic system explains fast phase of dynamic regulation of oxidative phosphorylation." American Journal of Physiology-Cell Physiology 293, no. 3 (2007): C815—C829. http://dx.doi.org/10.1152/ajpcell.00355.2006.

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Computational models of a large metabolic system can be assembled from modules that represent a biological function emerging from interaction of a small subset of molecules. A “skeleton model” is tested here for a module that regulates the first phase of dynamic adaptation of oxidative phosphorylation (OxPhos) to demand in heart muscle cells. The model contains only diffusion, mitochondrial outer membrane (MOM) permeation, and two isoforms of creatine kinase (CK), in cytosol and mitochondrial intermembrane space (IMS), respectively. The communication with two neighboring modules occurs via sti

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15

Holde, K. van, and T. Yager. "Models for chromatin remodeling: a critical comparison." Biochemistry and Cell Biology 81, no. 3 (2003): 169–72. http://dx.doi.org/10.1139/o03-038.

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Nucleosome remodeling has been shown, in many cases, to involve cis displacement of nucleosomes on the DNA. This process seems similar to the long-recognized random diffusion of nucleosomes along DNA, but the remodeling process is unidirectional and ATP dependent. Several years ago, we developed a model for nucleosome migration, based on the diffusion of "twist-defects" within the nucleosomal DNA. This has been modified into a model that incorporates ATP-dependent defect generation, and can account for many observations concerning remodeling. However, certain experimental studies in recent yea

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16

Illaste, Ardo, Martin Laasmaa, David Schryer, Rikke Birkedal, Pearu Peterson, and Marko Vendelin. "Determination of Regional Diffusion Coefficients of Fluorescent ATP in Rat Cardiomyocytes." Biophysical Journal 98, no. 3 (2010): 749a. http://dx.doi.org/10.1016/j.bpj.2009.12.4107.

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17

Li, Jia-Jia, Meng-Meng Du, Rong Wang, Jin-Zhi Lei, and Ying Wu. "Astrocytic Gliotransmitter: Diffusion Dynamics and Induction of Information Processing on Tripartite Synapses." International Journal of Bifurcation and Chaos 26, no. 08 (2016): 1650138. http://dx.doi.org/10.1142/s0218127416501388.

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Astrocytes have important functions in the central nervous system (CNS) and are significant in our understanding of the neuronal network. Astrocytes modulate neuronal firings at both single cell level of tripartite synapses and the neuron-glial network level. Astrocytes release adenosine triphosphate (ATP) and glutamate into the neuron-glial network. These gliotransmitters diffuse over the network to form long distance signals to regulate neuron firings. In this paper, we study a neuron-glial network model that includes a diffusion of astrocytic ATP and glutamate to investigate how long distan

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18

Sidell, B. D., and J. R. Hazel. "Temperature affects the diffusion of small molecules through cytosol of fish muscle." Journal of Experimental Biology 129, no. 1 (1987): 191–203. http://dx.doi.org/10.1242/jeb.129.1.191.

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Undiluted cytosolic extracts were prepared from fast glycolytic muscle tissue of white perch (Morone americanus). Diffusion coefficients (D) through the cytosol preparations were estimated in vitro for a series of selected low molecular weight compounds using an experimental diffusion chamber. Determinations were made at 5 degrees and 25 degrees C to assess thermal sensitivity of the process. Non-metabolizable analogues of naturally occurring compounds were employed to avoid chemical alteration of solutes by the catalytically competent preparations during diffusion experiments. Kinematic visco

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19

Baylor, S. M., and S. Hollingworth. "Model of Sarcomeric Ca2+ Movements, Including ATP Ca2+ Binding and Diffusion, during Activation of Frog Skeletal Muscle." Journal of General Physiology 112, no. 3 (1998): 297–316. http://dx.doi.org/10.1085/jgp.112.3.297.

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Cannell and Allen (1984. Biophys. J. 45:913–925) introduced the use of a multi-compartment model to estimate the time course of spread of calcium ions (Ca2+) within a half sarcomere of a frog skeletal muscle fiber activated by an action potential. Under the assumption that the sites of sarcoplasmic reticulum (SR) Ca2+ release are located radially around each myofibril at the Z line, their model calculated the spread of released Ca2+ both along and into the half sarcomere. During diffusion, Ca2+ was assumed to react with metal-binding sites on parvalbumin (a diffusible Ca2+- and Mg2+-binding pr

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20

Kargacin, M. E., and G. J. Kargacin. "Predicted changes in concentrations of free and bound ATP and ADP during intracellular Ca2+signaling." American Journal of Physiology-Cell Physiology 273, no. 4 (1997): C1416—C1426. http://dx.doi.org/10.1152/ajpcell.1997.273.4.c1416.

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High Ca2+ concentrations can develop near Ca2+ sources during intracellular signaling and might lead to localized regulation of Ca2+-dependent processes. By shifting the amount of Ca2+ and other cations associated with ATP, local high Ca2+ concentrations might also alter the substrate available for membrane-associated and cytoplasmic enzymes. To study this, simultaneous equations were solved over a range of Ca2+ and Mg2+ concentrations to determine the general effects of Ca2+ on the concentrations of free and Ca2+- and Mg2+-bound forms of ATP. To obtain a more specific picture of the changes t

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21

Leitch, Jeffry M., and Anthony Carruthers. "ATP-dependent sugar transport complexity in human erythrocytes." American Journal of Physiology-Cell Physiology 292, no. 2 (2007): C974—C986. http://dx.doi.org/10.1152/ajpcell.00335.2006.

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Human erythrocyte glucose sugar transport was examined in resealed red cell ghosts under equilibrium exchange conditions ([sugar]intracellular = [sugar]extracellular, where brackets indicate concentration). Exchange 3- O-methylglucose (3MG) import and export are monophasic in the absence of cytoplasmic ATP but are biphasic when ATP is present. Biphasic exchange is observed as the rapid filling of a large compartment (66% cell volume) followed by the slow filling of the remaining cytoplasmic space. Biphasic exchange at 20 mM 3MG eliminates the possibility that the rapid exchange phase represent

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22

Simson, Päivo, Marko Vendelin, and Pearu Peterson. "Using Raster Image Correlation Spectroscopy for the Detection of ADP/ATP Diffusion Restrictions in Rat Cardiomyocytes." Biophysical Journal 104, no. 2 (2013): 509a. http://dx.doi.org/10.1016/j.bpj.2012.11.2813.

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23

Alekseev, Alexey E., Rita Guzun, Santiago Reyes, et al. "Restrictions in ATP diffusion within sarcomeres can provoke ATP-depleted zones impairing exercise capacity in chronic obstructive pulmonary disease." Biochimica et Biophysica Acta (BBA) - General Subjects 1860, no. 10 (2016): 2269–78. http://dx.doi.org/10.1016/j.bbagen.2016.04.018.

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24

Borejdo, J., and S. Burlacu. "Diffusion of heavy meromyosin in the presence of F-actin and ATP." Journal of Muscle Research and Cell Motility 13, no. 1 (1992): 106–16. http://dx.doi.org/10.1007/bf01738434.

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25

Aw, T. Y., and D. P. Jones. "ATP concentration gradients in cytosol of liver cells during hypoxia." American Journal of Physiology-Cell Physiology 249, no. 5 (1985): C385—C392. http://dx.doi.org/10.1152/ajpcell.1985.249.5.c385.

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The activities of two ATP-requiring systems with different subcellular localizations were studied in cells in which average cellular ATP concentration was varied. The cytosolic ATP-sulfurylase activity varied linearly with the cellular ATP concentration; however, the plasma membrane Na+-K+-ATPase was substantially more sensitive to decreased ATP concentration. Under conditions where the cellular ATP concentration was lowered to 40% of control, Rb+ uptake was nearly zero. The results indicate that ATP-utilizing enzymes located in the plasma membrane in liver cells are exposed to a lower ATP con

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26

Hubley, M. J., B. R. Locke, and T. S. Moerland. "Reaction-diffusion analysis of the effects of temperature on high-energy phosphate dynamics in goldfish skeletal muscle." Journal of Experimental Biology 200, no. 6 (1997): 975–88. http://dx.doi.org/10.1242/jeb.200.6.975.

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Thermal acclimation results in dramatic changes in the fractional volume of mitochondria within skeletal muscle of teleost fish. We investigated the hypothesis that changes in mitochondrial volume represent a compensatory response to temperature-induced changes in intracellular diffusion coefficients (D) of the high-energy phosphate compounds ATP and creatine phosphate (PCr). Using 31P nuclear magnetic resonance spectroscopy, we determined DPCr and DATP in goldfish (Carassius auratus) skeletal muscle at 25 degrees C and 5 degrees C: DPCr was 3.28 +/- 0.18 x 10(-6) cm2s-1 at 25 degrees C and 2.

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27

Schmidt, Günter, and Peter Gräber. "The Rate of ATP Hydrolysis Catalyzed by Reconstituted CF0F1-Liposomes." Zeitschrift für Naturforschung C 42, no. 3 (1987): 231–36. http://dx.doi.org/10.1515/znc-1987-0311.

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The conditions for optimal rates of ATP hydrolysis catalyzed by the chloroplast ATP-synthase (ATPase), CF0F1, after isolation and reconstitution into asolectin liposomes have been investigated. The rate of ATP hydrolysis was measured either after oxidation of CF0F1 (by incubation with iodosobenzoate) or after reduction of CFoF, (by incubation with dithiothreitol). In both cases a rate of about 1-2 ATP (CF0F1·s)-1 was observed under uncoupled conditions. If the proteoliposomes are first energized by an acid-base transition and a K+ /valinomycin diffusion potential, the uncoupled rate of ATP h

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28

DEETJEN, PHILIPP, JÖRG THOMAS, HEIKO LEHRMANN, SUNG JOON KIM, and JENS LEIPZIGER. "The Luminal P2Y Receptor in the Isolated Perfused Mouse Cortical Collecting Duct." Journal of the American Society of Nephrology 11, no. 10 (2000): 1798–806. http://dx.doi.org/10.1681/asn.v11101798.

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Abstract. Extracellular nucleotides regulate renal ion transport. With the use of in vitro perfusion and [Ca2+]i imaging, this study investigated whether mouse and rabbit cortical collecting ducts (CCD) respond to luminal nucleotides. In mouse CCD, luminal ATP (EC50: 10 μM) and UTP (EC50: 9.7 μM) increased [Ca2+]i with an initial peak and a plateau. To make certain that basolateral P2 receptors were not activated by luminal nucleotides via leak diffusion, luminal trypsin (1 μM), a known agonist for basolateral proteinase-activated receptors, was perfused. Mouse CCD that were responsive to lumi

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29

Szczechowicz, A., L. Hryniewiecka, and H. Kmita. "The influence of depletion of voltage dependent anion selective channel on protein import into the yeast Saccharomyces cerevisiae mitochondria." Acta Biochimica Polonica 48, no. 3 (2001): 719–28. http://dx.doi.org/10.18388/abp.2001_3906.

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The supply of substrates to the respiratory chain as well as of other metabolites (e.g. ATP) into inner compartments of mitochondria is crucial to preprotein import into these organelles. Transport of the compounds across the outer mitochondrial membrane is enabled by mitochondrial porin, also known as the voltage-dependent anion-selective channel (VDAC). Our previous studies led to the conclusion that the transport of metabolites through the outer membrane of the yeast Saccharomyces cerevisiae mitochondria missing VDAC (now termed YVDAC1) is considerably restricted. Therefore we expected that

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Cui, Haodong, Min Jiang, Wenhua Zhou, et al. "Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma." Biology 10, no. 10 (2021): 1038. http://dx.doi.org/10.3390/biology10101038.

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A carrier-free CRISPR/Cas9 ribonucleoprotein delivery strategy for genome editing mediated by a cold atmospheric plasma (CAP) is described. The CAP is promising in many biomedical applications due to efficient production of bioactive ionized species. The MCF-7 cancer cells after CAP exposure exhibit increased extracellular reactive oxygen and nitrogen species (RONS) and altered membrane potential and permeability. Hence, transmembrane transport of Ca2+ into the cells increases and accelerates ATP hydrolysis, resulting in enhanced ATP-dependent endocytosis. Afterwards, the increased Ca2+ and AT

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31

Mitchell, Beth F., Lotte B. Pedersen, Michael Feely, Joel L. Rosenbaum, and David R. Mitchell. "ATP Production inChlamydomonas reinhardtiiFlagella by Glycolytic Enzymes." Molecular Biology of the Cell 16, no. 10 (2005): 4509–18. http://dx.doi.org/10.1091/mbc.e05-04-0347.

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Eukaryotic cilia and flagella are long, thin organelles, and diffusion from the cytoplasm may not be able to support the high ATP concentrations needed for dynein motor activity. We discovered enzyme activities in the Chlamydomonas reinhardtii flagellum that catalyze three steps of the lower half of glycolysis (phosphoglycerate mutase, enolase, and pyruvate kinase). These enzymes can generate one ATP molecule for every substrate molecule consumed. Flagellar fractionation shows that enolase is at least partially associated with the axoneme, whereas phosphoglycerate mutase and pyruvate kinase pr

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32

Saks, V. A., Y. O. Belikova, A. V. Kuznetsov, et al. "Phosphocreatine pathway for energy transport: ADP diffusion and cardiomyopathy." American Journal of Physiology-Lung Cellular and Molecular Physiology 261, no. 4 (1991): L30—L38. http://dx.doi.org/10.1152/ajplung.1991.261.4.l30.

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Chemically skinned (by treatment with saponin, 40 μg/ml) isolated cardiomyocytes were used to study the intracellular diffusion of ADP and creatine (Cr). Stimulation of respiration was studied in these cardiomyocytes without intact sarcolemma and in isolated heart mitochondrial by addition of ADP and Cr in the presence of 0.2 mM ATP (via mitochondrial creatine kinase reaction: Cr + MgATP = MgADP + PCr). The Michaelis constant (Km) for Cr was similar in both cases, 5.67 ± 0.11 (SD) mM in skinned myocytes and 6.9 ± 0.2 mM in mitochondria, showing that there is no significant restriction to the d

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Saks, V. A., Y. O. Belikova, A. V. Kuznetsov, et al. "Phosphocreatine pathway for energy transport: ADP diffusion and cardiomyopathy." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 4 (1991): 30–38. http://dx.doi.org/10.1152/ajpheart.1991.261.4.30.

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Chemically skinned (by treatment with saponin, 40 μg/ml) isolated cardiomyocytes were used to study the intracellular diffusion of ADP and creatine (Cr). Stimulation of respiration was studied in these cardiomyocytes without intact sarcolemma and in isolated heart mitochondrial by addition of ADP and Cr in the presence of 0.2 mM ATP (via mitochondrial creatine kinase reaction: Cr + MgATP = MgADP + PCr). The Michaelis constant (Km) for Cr was similar in both cases, 5.67 ± 0.11 (SD) mM in skinned myocytes and 6.9 ± 0.2 mM in mitochondria, showing that there is no significant restriction to the d

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34

Lai, Nicola, Jessica Spires, Bruno Grassi, L. Bruce Gladden, and Gerald Saidel. "Analysis Of Oxygen Diffusion Limitation In Contracting Skeletal Muscle During Higher ATP Demand." Medicine & Science in Sports & Exercise 42 (October 2010): 23–24. http://dx.doi.org/10.1249/01.mss.0000389530.44261.f5.

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Hardy, Kristin M., Richard M. Dillaman, Bruce R. Locke, and Stephen T. Kinsey. "A skeletal muscle model of extreme hypertrophic growth reveals the influence of diffusion on cellular design." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 296, no. 6 (2009): R1855—R1867. http://dx.doi.org/10.1152/ajpregu.00076.2009.

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Muscle fibers that power swimming in the blue crab Callinectes sapidus are <80 μm in diameter in juveniles but grow hypertrophically, exceeding 600 μm in adults. Therefore, intracellular diffusion distances become progressively greater as the animals grow and, in adults, vastly exceed those in most cells. This developmental trajectory makes C. sapidus an excellent model for characterization of the influence of diffusion on fiber structure. The anaerobic light fibers, which power burst swimming, undergo a prominent shift in organelle distribution with growth. Mitochondria, which require O2 a

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36

Gerencser, G. A. "Electrogenic ATP-dependent Cl- transport by plasma membrane vesicles from Aplysia intestine." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 254, no. 1 (1988): R127—R133. http://dx.doi.org/10.1152/ajpregu.1988.254.1.r127.

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A Cl--stimulated adenosinetriphosphatase (ATPase) activity and an ATP-dependent Cl- transport process were found in Aplysia enterocyte plasma membranes. In an attempt to further elucidate this transport process plasma membrane vesicles from Aplysia enterocytes were prepared utilizing differential centrifugation and sucrose density gradient techniques. Electrogenicity of the ATP-dependent Cl- transport was confirmed in three ways. First, an inwardly directed valinomycin-induced K+ diffusion potential, making the vesicle interior electrically positive, enhanced ATP-driven Cl- uptake compared wit

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37

Ogura, Teru, Yuka Matsushita-Ishiodori, Ai Johjima, et al. "From the common molecular basis of the AAA protein to various energy-dependent and -independent activities of AAA proteins." Biochemical Society Transactions 36, no. 1 (2008): 68–71. http://dx.doi.org/10.1042/bst0360068.

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AAA (ATPase associated with various cellular activities) proteins remodel substrate proteins and protein complexes upon ATP hydrolysis. Substrate remodelling is diverse, e.g. proteolysis, unfolding, disaggregation and disassembly. In the oligomeric ring of the AAA protein, there is a conserved aromatic residue which lines the central pore. Functional analysis indicates that this conserved residue in AAA proteases is involved in threading unfolded polypeptides. Katanin and spastin have microtubule-severing activity. These AAA proteins also possess a conserved aromatic residue at the central por

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Abaturova, Anna M., Nadezda A. Brazhe, Ilya B. Kovalenko, Galina Yu Riznichenko, and Andrei B. Rubin. "Brownian dynamics simulation of cytochrome c diffusion and binding with cytochrome c1 in mitochondrial crista." ITM Web of Conferences 31 (2020): 04001. http://dx.doi.org/10.1051/itmconf/20203104001.

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Cytochrome c (Cc) protein shuttles electrons from respiratory chain complex III — from cytochrome c1 (Cc1) subunit — to complex IV during oxidative phosphorylation, in intermembrane space of mitochondria and cristae lumen. With Leigh syndrome (LS), the crista lumen width (CLW) increases, and ATP production declines. One of the questions raised by this situation is to find out how ATP production impairs at LS. Using the simulation of Brownian dynamics, we tested whether the increase in CLW declines respiration at the stage of electron transport of Cc to Cc1. We designed a Brownian dynamics mode

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Johnson, Matthew P. "Metabolic regulation of photosynthetic membrane structure tunes electron transfer function." Biochemical Journal 475, no. 7 (2018): 1225–33. http://dx.doi.org/10.1042/bcj20170526.

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The photosynthetic chloroplast thylakoid membrane of higher plants is a complex three-dimensional structure that is morphologically dynamic on a timescale of just a few minutes. The membrane dynamics are driven by the phosphorylation of light-harvesting complex II (LHCII) by the STN7 kinase, which controls the size of the stacked grana region relative to the unstacked stromal lamellae region. Here, I hypothesise that the functional significance of these membrane dynamics is in controlling the partition of electrons between photosynthetic linear and cyclic electron transfer (LET and CET), which

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Calonge, M. L., A. Ilundain, and J. Bolufer. "Glycyl-L-sarcosine transport by ATP-depleted isolated enterocytes from chicks." American Journal of Physiology-Gastrointestinal and Liver Physiology 259, no. 5 (1990): G775—G780. http://dx.doi.org/10.1152/ajpgi.1990.259.5.g775.

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Normally energized and ATP-depleted isolated chicken enterocytes have been used to investigate the energetics of intestinal glycyl-L-sarcosine (Gly-Sar) transport, and the results were compared with those obtained for 3-O-methyl-D-glucose (3-OMG) active transport, which is known to be energized by the electrochemical Na+ gradient. The results show that even though Gly-Sar had no effect on Na+ efflux from labeled (22Na) enterocytes, 20 mM L-leucine and 20 mM 3-OMG increased the rate constant of Na+ efflux by 34 and 76%, respectively. In ATP-depleted cells 3-OMG was accumulated in response to ex

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de Graaf, Robin A., Arnaud van Kranenburg, and Klaas Nicolay. "In Vivo 31P-NMR Diffusion Spectroscopy of ATP and Phosphocreatine in Rat Skeletal Muscle." Biophysical Journal 78, no. 4 (2000): 1657–64. http://dx.doi.org/10.1016/s0006-3495(00)76717-8.

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Pessoa, Amanda C. S. N., Caroline C. Sipoli, and Lucimara G. de la Torre. "Effects of diffusion and mixing pattern on microfluidic-assisted synthesis of chitosan/ATP nanoparticles." Lab on a Chip 17, no. 13 (2017): 2281–93. http://dx.doi.org/10.1039/c7lc00291b.

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Vendelin, Marko, Ardo Illaste, and Rikke Birkedal. "Anisotropic Diffusion Of Fluorescently Labeled Atp In Cardiomyocytes Determined By Raster Image Correlation Spectroscopy." Biophysical Journal 96, no. 3 (2009): 624a. http://dx.doi.org/10.1016/j.bpj.2008.12.3300.

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Reyes, J. G., M. V. Velarde, R. Ugarte, and D. J. Benos. "Glycolytic component of rat spermatid energy and acid-base metabolism." American Journal of Physiology-Cell Physiology 259, no. 4 (1990): C660—C667. http://dx.doi.org/10.1152/ajpcell.1990.259.4.c660.

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The impact of glycolysis on rat spermatid energy metabolism is made apparent by the simultaneous occurrence of the following three events upon glucose addition to the extracellular medium of a rat spermatid cell suspension: decrease in ATP content, exit of acid equivalents, and increased lactate production and efflux. In this work, we have studied the interrelations between these three phenomena. By measuring ATP content, net acid transport, lactate exit, oxygen consumption, intracellular pH, CO2 production, and glycolytic intermediates in the presence of glucose and glucose analogues, we conc

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KOIZUMI, Schuichi, Kayoko FUJISHITA, Kaori INOUE, Yukari SHIGEMOTO-MOGAMI, Makoto TSUDA, and Kazuhide INOUE. "Ca2+ waves in keratinocytes are transmitted to sensory neurons: the involvement of extracellular ATP and P2Y2 receptor activation." Biochemical Journal 380, no. 2 (2004): 329–38. http://dx.doi.org/10.1042/bj20031089.

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ATP acts as an intercellular messenger in a variety of cells. In the present study, we have characterized the propagation of Ca2+ waves mediated by extracellular ATP in cultured NHEKs (normal human epidermal keratinocytes) that were co-cultured with mouse DRG (dorsal root ganglion) neurons. Pharmacological characterization showed that NHEKs express functional metabotropic P2Y2 receptors. When a cell was gently stimulated with a glass pipette, an increase in [Ca2+]i (intracellular Ca2+ concentration) was observed, followed by the induction of propagating Ca2+ waves in neighbouring cells in an e

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Stephenson, E. W. "Excitation of skinned muscle fibers by imposed ion gradients. II. Influence of quercetin and ATP removal on the Ca2+-insensitive component of stimulated 45Ca efflux." Journal of General Physiology 86, no. 6 (1985): 833–52. http://dx.doi.org/10.1085/jgp.86.6.833.

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Ionic gradients imposed by choline Cl replacement of K methanesulfonate (Mes) at constant [K][Cl] product stimulate 45Ca efflux from skinned muscle fibers; a small, sustained Ca2+-insensitive efflux component, observed in EGTA, appears to grade a much larger Ca2+-dependent component responsible for contractile activation and is likely to reflect intermediate steps in excitation-contraction coupling. The present studies examined ATP-related effects on the Ca2+-insensitive stimulation. 45Ca efflux was measured on segments of frog semitendinosus muscle skinned by microdissection, with isometric f

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Gao, Xinwei, Yanfeng Liu, Jia Zhang, et al. "Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy." Membranes 11, no. 11 (2021): 891. http://dx.doi.org/10.3390/membranes11110891.

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Although conventional fluorescence intensity imaging can be used to qualitatively study the drug toxicity of nanodrug carrier systems at the single-cell level, it has limitations for studying nanodrug transport across membranes. Fluorescence correlation spectroscopy (FCS) can provide quantitative information on nanodrug concentration and diffusion in a small area of the cell membrane; thus, it is an ideal tool for studying drug transport across the membrane. In this paper, the FCS method was used to measure the diffusion coefficients and concentrations of carbon dots (CDs), doxorubicin (DOX) a

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Pfanner, N., and W. Neupert. "Transport of proteins into mitochondria: a potassium diffusion potential is able to drive the import of ADP/ATP carrier." EMBO Journal 4, no. 11 (1985): 2819–25. http://dx.doi.org/10.1002/j.1460-2075.1985.tb04009.x.

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Block, Ethan R., and Jes K. Klarlund. "Wounding Sheets of Epithelial Cells Activates the Epidermal Growth Factor Receptor through Distinct Short- and Long-Range Mechanisms." Molecular Biology of the Cell 19, no. 11 (2008): 4909–17. http://dx.doi.org/10.1091/mbc.e08-01-0097.

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Wounding epithelia induces activation of the epidermal growth factor receptor (EGFR), which is absolutely required for induction of motility. ATP is released from cells after wounding; it binds to purinergic receptors on the cell surface, and the EGFR is subsequently activated. Exogenous ATP activates phospholipase D, and we show here that ATP activates the EGFR through the phospholipase D2 isoform. The EGFR is activated in cells far (>0.3 cm) from wounds, which is mediated by diffusion of extracellular ATP because activation at a distance from wounds is abrogated by eliminating ATP in the

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Chambon, Marc H., and Odile M. Viratelle. "Interaction of Doxorubicin with ATP: Quantification of Complexes and Effect on Its Diffusion into DNA-Loaded Liposomes—Implication for ATP-Driven Transport Studies." Analytical Biochemistry 263, no. 2 (1998): 198–207. http://dx.doi.org/10.1006/abio.1998.2790.

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