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Journal articles on the topic 'Digital droplet PCR'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Zhou, Wuping, Cong Liu, Tao Zhang, et al. "Low Cost, Easily-Assembled Centrifugal Buoyancy-Based Emulsification and Digital PCR." Micromachines 13, no. 2 (2022): 171. http://dx.doi.org/10.3390/mi13020171.

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Microfluidic-based droplet generation approaches require the design of microfluidic chips and a precise lithography process, which require skilled technicians and a long manufacturing time. Here we developed a centrifugal buoyancy-based emulsification (CBbE) method for producing droplets with high efficiency and minimal fabrication time. Our approach is to fabricate a droplet generation module that can be easily assembled using syringe needles and PCR tubes. With this module and a common centrifuge, high-throughput droplet generation with controllable droplet size could be realized in a few mi

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2

Schuler, Friedrich, Martin Trotter, Marcel Geltman, et al. "Digital droplet PCR on disk." Lab on a Chip 16, no. 1 (2016): 208–16. http://dx.doi.org/10.1039/c5lc01068c.

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3

Meng, Xiangkai, Yuanhua Yu, and Guangyong Jin. "Numerical Simulation and Experimental Verification of Droplet Generation in Microfluidic Digital PCR Chip." Micromachines 12, no. 4 (2021): 409. http://dx.doi.org/10.3390/mi12040409.

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The generation of droplets is one of the most critical steps in the droplet digital polymerase chain reaction (ddPCR) procedure. In this study, the mechanism of droplet formation in microchannel structure and factors affecting droplet formation were studied. The physical field of laminar two-phase flow level was used to simulate the process of droplet generation through microfluidic technology. The effect of the parameters including flow rate, surface tension, and viscosity on the generated droplet size were evaluated by the simulation. After that, the microfluidic chip that has the same dimen

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4

Fitarelli-Kiehl, Mariana, Fangyan Yu, Ravina Ashtaputre, et al. "Denaturation-Enhanced Droplet Digital PCR for Liquid Biopsies." Clinical Chemistry 64, no. 12 (2018): 1762–71. http://dx.doi.org/10.1373/clinchem.2018.293845.

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Abstract BACKGROUND Although interest in droplet-digital PCR technology (ddPCR) for cell-free circulating DNA (cfDNA) analysis is burgeoning, the technology is compromised by subsampling errors and the few clinical targets that can be analyzed from limited input DNA. The paucity of starting material acts as a “glass ceiling” in liquid biopsies because, irrespective how analytically sensitive ddPCR techniques are, detection limits cannot be improved past DNA input limitations. METHODS We applied denaturation-enhanced ddPCR (dddPCR) using fragmented genomic DNA (gDNA) with defined mutations. We

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Nugroho, Kristianto, Dwi Widyajayantie, Sayyidah Afridatul Ishthifaiyyah, and Elisa Apriliani. "Pemanfaatan Teknologi Droplet Digital PCR (ddPCR) dalam Kegiatan Analisis Molekuler Tanaman." JURNAL BIOS LOGOS 11, no. 1 (2021): 28. http://dx.doi.org/10.35799/jbl.11.1.2021.31101.

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(Article History: Received 23 October 2020; Revised 9 January 2021; Accepted 18 January 2021) ABSTRAKSelama beberapa dekade terakhir, teknik PCR memberikan manfaat yang begitu besar dalam kegiatan penelitian di bidang biologi molekuler. Digital droplet PCR (ddPCR) merupakan salah satu teknologi PCR terbaru yang diklaim memiliki keunggulan dibanding teknik qPCR. Prinsip kerja teknik ini yaitu membagi sampel menjadi molekul-molekul kecil yang dipisahkan oleh emulsi minyak, air, dan senyawa penstabil sehingga membentuk droplets. Teknik ini memiliki kelebihan mampu melakukan kuantifikasi absolut m

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6

Dutra, Lara, Ole Franz, Veli-Mikko Puupponen, and Marja Tiirola. "DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen." BioTechniques 69, no. 6 (2020): 450–54. http://dx.doi.org/10.2144/btn-2020-0076.

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Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase se

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7

Luo, Gangyin, Ying Zhang, Shun Wang, Xinbei Lv, Tianhang Yang, and Jinxian Wang. "Establishment and Validation of an Integrated Microfluidic Step Emulsification Chip Supporting Droplet Digital Nucleic Acid Analysis." Biosensors 13, no. 9 (2023): 888. http://dx.doi.org/10.3390/bios13090888.

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Uniform and stable droplet generation is critical for accurate and efficient digital nucleic acid analysis (dNAA). In this study, an integrated microfluidic step emulsification device with wide-range droplet generation capability, small device dimensions, convenient fabrication strategy, low contamination and high robustness was developed. A tree-shaped droplet generation nozzle distribution design was proposed to increase the uniformity of droplet generation by equating flow rates, and the flow field in the design was numerically simulated. Theoretical analysis and comparative experiments on

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8

Rausch, Christian, Maja Rothenberg-Thurley, Simon A. Buerger, et al. "Double Drop-Off Droplet Digital PCR." Journal of Molecular Diagnostics 23, no. 8 (2021): 975–85. http://dx.doi.org/10.1016/j.jmoldx.2021.05.001.

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9

Lo Schirico, Mariella, Martina Ferrante, Irene Dogliotti, et al. "Droplet Digital PCR Assay for MYD88L265P." HemaSphere 4, no. 1 (2020): e324. http://dx.doi.org/10.1097/hs9.0000000000000324.

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10

Moser, Dirk A., Luca Braga, Andrea Raso, Serena Zacchigna, Mauro Giacca, and Perikles Simon. "Transgene Detection by Digital Droplet PCR." PLoS ONE 9, no. 11 (2014): e111781. http://dx.doi.org/10.1371/journal.pone.0111781.

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11

Ragni, Margaret V. "Prenatal diagnosis by droplet digital PCR." Blood 130, no. 3 (2017): 240–41. http://dx.doi.org/10.1182/blood-2017-05-786269.

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12

LIU Cong, 刘. 聪., 董文飞 DONG Wen-fei, 张. 涛. ZHANG Tao, 周武平 ZHOU Wu-ping, 蒋克明 JIANG Ke-ming, and 黎海文 LI Hai-wen. "Identification of florescent droplets at low concentrations for droplet digital PCR." Optics and Precision Engineering 26, no. 3 (2018): 647–53. http://dx.doi.org/10.3788/ope.20182603.0647.

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13

Dutka, Filip, Adam S. Opalski, and Piotr Garstecki. "Nano-liter droplet libraries from a pipette: step emulsificator that stabilizes droplet volume against variation in flow rate." Lab on a Chip 16, no. 11 (2016): 2044–49. http://dx.doi.org/10.1039/c6lc00265j.

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Many modern analytical assays, for example, droplet digital PCR, or screening of the properties of single cells or single mutated genes require splitting a liquid sample into a number of small (typically ca. nano-liter in volume) independent compartments or droplets.

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14

Maier, Jacqueline, Thoralf Lange, Michael Cross, Kathrin Wildenberger, Dietger Niederwieser, and Georg-Nikolaus Franke. "Optimized Digital Droplet PCR for BCR-ABL." Journal of Molecular Diagnostics 21, no. 1 (2019): 27–37. http://dx.doi.org/10.1016/j.jmoldx.2018.08.012.

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15

Veach, Alexander J., Clayton Beard, Frederick Porter, Mark Wilson, and Francesco Berlanda Scorza. "Digital Droplet PCR for Influenza Vaccine Development." Procedia in Vaccinology 9 (2015): 96–103. http://dx.doi.org/10.1016/j.provac.2015.05.014.

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16

Wu, Zerui, Wanjun Yao, Jinyu Chen, et al. "Droplet digital PCR-based single aptamer selection." Talanta 292 (September 2025): 127924. https://doi.org/10.1016/j.talanta.2025.127924.

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17

Martinez, Ryan, Davis Nennig, and Pawel Mroz. "Quantification of BCR-ABL1 Fusion Transcripts in Patients With Acute B Lymphoblastic Leukemia by Multiplex Droplet Digital PCR." American Journal of Clinical Pathology 152, Supplement_1 (2019): S135. http://dx.doi.org/10.1093/ajcp/aqz126.003.

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Abstract Droplet digital PCR (ddPCR) is a novel PCR platform that provides quantification of amplification targets by massive sample partitioning and analysis in a single sample. These results are independent of a calibration curve and may increase detection when compared to quantitative RT-PCR (qRT-PCR). The t(9;22)(BCR-ABL1) translocation is associated with chronic myelogenous leukemia (CML) and a subset of acute B lymphoblastic leukemias (B-ALLs). Several studies have demonstrated the clinical utility of quantifying BCR-ABL1 fusion transcript levels for detecting minimal residual disease (M

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18

Gutierrez, Kimberley, Benjamin Foret, Cécile Jovelet, and Allison C. Mallory. "Abstract 2942: Robust sample recovery post-digital PCR for downstream genomic applications." Cancer Research 82, no. 12_Supplement (2022): 2942. http://dx.doi.org/10.1158/1538-7445.am2022-2942.

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Abstract Droplet microfluidics technologies, and particularly, digital PCR have provided highly precise and sensitive detection of genetic targets. There is a strong interest for researchers to reuse sample due to sample scarcity, as well as, to further characterize or validate their experimental findings. Historically, recovery of PCR products for downstream genomic testing applications has been challenging due to the stability of the sample post-reaction. Stilla Technologies has developed Crystal Digital PCR™ and the naica® system, a flexible and highplex droplet and imaging-based digital PC

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19

Nasser, Gamal A., Ahmed M. R. Fath El-Bab, Ahmed L. Abdel-Mawgood, Hisham Mohamed, and Abdelatty M. Saleh. "CO2 Laser Fabrication of PMMA Microfluidic Double T-Junction Device with Modified Inlet-Angle for Cost-Effective PCR Application." Micromachines 10, no. 10 (2019): 678. http://dx.doi.org/10.3390/mi10100678.

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The formation of uniform droplets and the control of their size, shape and monodispersity are of utmost importance in droplet-based microfluidic systems. The size of the droplets is precisely tuned by the channel geometry, the surface interfacial tension, the shear force and fluid velocity. In addition, the fabrication technique and selection of materials are essential to reduce the fabrication cost and time. In this paper, for reducing the fabrication cost Polymethyl methacrylate (PMMA) sheet is used with direct write laser technique by VERSA CO2 laser VLS3.5. This laser writing technique giv

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20

Chen, Jinyu, Zhaofeng Luo, Lin Li, et al. "Capillary-based integrated digital PCR in picoliter droplets." Lab on a Chip 18, no. 3 (2018): 412–21. http://dx.doi.org/10.1039/c7lc01160a.

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21

Kokkoris, Vasilis, Eric Vukicevich, Andrew Richards, Corrina Thomsen, and Miranda M. Hart. "Challenges Using Droplet Digital PCR for Environmental Samples." Applied Microbiology 1, no. 1 (2021): 74–88. http://dx.doi.org/10.3390/applmicrobiol1010007.

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Droplet digital polymerase chain reaction (ddPCR) is a method used to detect and quantify nucleic acids even when present in exceptionally low numbers. While it has proven to be valuable for clinical studies, it has failed to be widely adopted for environmental studies but despite some limitations, ddPCR may represent a better option than classical qPCR for environmental samples. Due to the complexity of the chemical and biological composition of environmental samples, protocols tailored to clinical studies are not appropriate, and results are difficult to interpret. We used environmental DNA

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22

Park, Juhwan, Kyoung G. Lee, Dong Hyun Han, Ji-Soo Lee, Seok Jae Lee, and Je-Kyun Park. "Pushbutton-activated microfluidic dropenser for droplet digital PCR." Biosensors and Bioelectronics 181 (June 2021): 113159. http://dx.doi.org/10.1016/j.bios.2021.113159.

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23

Chantran, Y., S. Barete, P. Hirsch, and M. Arock. "Hereditary alpha tryptasemia determination by digital droplet PCR." Revue Française d'Allergologie 62, no. 3 (2022): 308. http://dx.doi.org/10.1016/j.reval.2022.02.046.

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24

Blagodatova, A. V., K. V. Kochkina, M. A. Komarova, N. Y. Trofina, M. M. Petrova, and A. V. Protopopov. "Aptamers selection for platelets using droplet digital PCR." Siberian Medical Review, no. 2 (2021): 100–103. http://dx.doi.org/10.20333/25000136-2021-2-100-103.

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The aim of the research. To obtain aptamers-inhibitors of platelet glycoprotein IIb / IIIa receptors, blocking platelet aggregation. Material and methods. Th e selection of aptamers for IIb / IIIa receptors of platelets was carried out according to the SELEX method (Systematic Evolution of Ligands by Exponential Enrichment), modifi ed to select aptamers for a specifi c epitope. Th e method allows selection and in vitro evolution of aptamers with selectivity to a specifi c target from a large library of oligonucleotides. Th e affi nity of aptamers for platelet IIb / IIIa receptors was determine

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25

Nicot, Florence, Michelle Cazabat, Sébastien Lhomme, et al. "Quantification of HEV RNA by Droplet Digital PCR." Viruses 8, no. 8 (2016): 233. http://dx.doi.org/10.3390/v8080233.

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26

Nyaruaba, Raphael, Caroline Mwaliko, Kelvin Kimutai Kering, and Hongping Wei. "Droplet digital PCR applications in the tuberculosis world." Tuberculosis 117 (July 2019): 85–92. http://dx.doi.org/10.1016/j.tube.2019.07.001.

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27

Romsos, Erica L., and Peter M. Vallone. "Estimation of extraction efficiency by droplet digital PCR." Forensic Science International: Genetics Supplement Series 7, no. 1 (2019): 515–17. http://dx.doi.org/10.1016/j.fsigss.2019.10.072.

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28

Hou, Ying, Shulang Chen, Yajing Zheng, Xiaonan Zheng, and Jin-Ming Lin. "Droplet-based digital PCR (ddPCR) and its applications." TrAC Trends in Analytical Chemistry 158 (January 2023): 116897. http://dx.doi.org/10.1016/j.trac.2022.116897.

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29

Hindson, Christopher M., John R. Chevillet, Hilary A. Briggs, et al. "Absolute quantification by droplet digital PCR versus analog real-time PCR." Nature Methods 10, no. 10 (2013): 1003–5. http://dx.doi.org/10.1038/nmeth.2633.

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30

Shi, Bing, Di Wu, Yangyang Jiang, Junru An, and Wenming Wu. "Off‐Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR." Advanced Materials Interfaces 7, no. 22 (2020): 2001074. http://dx.doi.org/10.1002/admi.202001074.

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31

Dai, Xiaoqing, Meng Cao, and Zunliang Wang. "Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR." Biosensors 15, no. 1 (2025): 36. https://doi.org/10.3390/bios15010036.

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We present a cost-effective and simple multiplex nucleic acid quantification method using droplet digital PCR (ddPCR) with digital melting curve analysis (MCA). This approach eliminates the need for complex fluorescent probe design, reducing both costs and dependence on fluorescence channels. We developed a convolutional neighborhood search algorithm to correct droplet displacement during heating, ensuring precise tracking and accurate extraction of melting curves. An experimental protocol for digital MCA on the ddPCR platform was established, enabling accurate quantification of six target pat

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32

Kaeda, Jaspal, Simone Bonecker, Frauke Ringel, et al. "Droplet Digital PCR Reliably Detects a Single Copy of BCR-ABL1." Blood 126, no. 23 (2015): 2784. http://dx.doi.org/10.1182/blood.v126.23.2784.2784.

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Abstract Data show 40% of chronic myeloid leukemia (CML) patients maintain complete molecular remission (CMR), i.e. failure to detect BCR-ABL1, consenting to termination of Imatinib mesylate (IM) therapy, following undetectable disease for ≥2 years by quantitative PCR (Q-PCR). These findings suggest majority of the patients experience molecular relapse. Furthermore, majority relapse in the first 6 months, implying Q-PCR assay sensitivity is suboptimum, to confidently identify patients for discontinuation of IM. Droplet digital PCR (ddPCR) is suggested to have sensitivity that is one log greate

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33

Iribarnegaray, Victoria, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, and Rodrigo Puentes. "Droplet Digital PCR Enhances Sensitivity of Canine Distemper Virus Detection." Viruses 16, no. 11 (2024): 1720. http://dx.doi.org/10.3390/v16111720.

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Canine distemper virus (CDV) poses a substantial threat to diverse carnivorans, leading to systemic and often fatal diseases. Accurate and prompt diagnosis is paramount for effective management and curbing further transmission. This study evaluates the diagnostic performance of droplet digital PCR (ddPCR) in comparison to conventional reverse-transcription (RT-PCR) and quantitative reverse-transcription real-time PCR (RT-qPCR). Seventy-six clinical samples were collected from dogs with CDV symptoms diagnosed by specialized veterinarians, and sixteen samples from apparently healthy individuals.

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34

Wang, Kangning, Bin Li, and Wenming Wu. "Compressed Air-Driven Continuous-Flow Thermocycled Digital PCR for HBV Diagnosis in Clinical-Level Serum Sample Based on Single Hot Plate." Molecules 25, no. 23 (2020): 5646. http://dx.doi.org/10.3390/molecules25235646.

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We report a novel compressed air-driven continuous-flow digital PCR (dPCR) system based on a 3D microfluidic chip and self-developed software system to realize real-time monitoring. The system can ensure the steady transmission of droplets in long tubing without an external power source and generate stable droplets of suitable size for dPCR by two needles and a narrowed Teflon tube. The stable thermal cycle required by dPCR can be achieved by using only one constant temperature heater. In addition, our system has realized the real-time detection of droplet fluorescence in each thermal cycle, w

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35

Lee, Cherl-Joon, Wonseok Shin, Minsik Song, et al. "Comparison of digital PCR platforms using the molecular marker." Genomics & Informatics 21, no. 2 (2023): e24. http://dx.doi.org/10.5808/gi.23008.

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Assays of clinical diagnosis and species identification using molecular markers are performed according to a quantitative method in consideration of sensitivity, cost, speed, convenience, and specificity. However, typical polymerase chain reaction (PCR) assay is difficult to quantify and have various limitations. In addition, to perform quantitative analysis with the quantitative real-time PCR (qRT-PCR) equipment, a standard curve or normalization using reference genes is essential. Within the last a decade, previous studies have reported that the digital PCR (dPCR) assay, a third-generation P

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36

George, David, Juliann Czech, Bobby John, Min Yu, and Lawrence J. Jennings. "Detection and quantification of chimerism by droplet digital PCR." Chimerism 4, no. 3 (2013): 102–8. http://dx.doi.org/10.4161/chim.25400.

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37

Karlin-Neumann, George. "Droplet Digital PCR Primes Liquid Biopsies for the Clinic." Clinical OMICs 1, no. 8 (2014): 28–29. http://dx.doi.org/10.1089/clinomi.01.08.09.

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38

Decraene, Charles, Amanda B. Silveira, François-Clément Bidard, et al. "Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR." Clinical Chemistry 64, no. 2 (2018): 317–28. http://dx.doi.org/10.1373/clinchem.2017.272518.

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Abstract BACKGROUND Progress in the liquid biopsy field, combined with the development of droplet digital PCR (ddPCR), has enabled noninvasive monitoring of mutations with high detection accuracy. However, current assays detect a restricted number of mutations per reaction. ddPCR is a recognized method for detecting alterations previously characterized in tumor tissues, but its use as a discovery tool when the mutation is unknown a priori remains limited. METHODS We established 2 ddPCR assays detecting all genomic alterations within KRAS exon 2 and EGFR exon 19 mutation hotspots, which are of

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39

Dewispelaere, Laurent, Leonore Bleret, Tom Van Acker, et al. "One-Step Duplex Droplet Digital PCR for WT1 Overexpression." Journal of Molecular Diagnostics 22, no. 8 (2020): 1008–19. http://dx.doi.org/10.1016/j.jmoldx.2020.05.010.

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40

Srisutham, Suttipat, Naowarat Saralamba, Benoit Malleret, Laurent Rénia, Arjen M. Dondorp, and Mallika Imwong. "Four human Plasmodium species quantification using droplet digital PCR." PLOS ONE 12, no. 4 (2017): e0175771. http://dx.doi.org/10.1371/journal.pone.0175771.

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41

Nshimyimana, Jean Pierre, Mercedes C. Cruz, Stefan Wuertz, and Janelle R. Thompson. "Variably improved microbial source tracking with digital droplet PCR." Water Research 159 (August 2019): 192–202. http://dx.doi.org/10.1016/j.watres.2019.04.056.

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42

Preobrazhenskaya, Elena V., Ilya V. Bizin, Ekatherina Sh Kuligina, et al. "Detection of BRCA1 gross rearrangements by droplet digital PCR." Breast Cancer Research and Treatment 165, no. 3 (2017): 765–70. http://dx.doi.org/10.1007/s10549-017-4357-7.

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43

Wang, Jing, Yi-ying Zhao, Jian-feng Li, et al. "IDH1 mutation detection by droplet digital PCR in glioma." Oncotarget 6, no. 37 (2015): 39651–60. http://dx.doi.org/10.18632/oncotarget.5630.

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44

Sedlak, R. H., L. Cook, A. Cheng, A. Magaret, and K. R. Jerome. "Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus." Journal of Clinical Microbiology 52, no. 8 (2014): 2844–48. http://dx.doi.org/10.1128/jcm.00803-14.

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45

Anderson, Elizabeth M., and Frank Maldarelli. "Quantification of HIV DNA Using Droplet Digital PCR Techniques." Current Protocols in Microbiology 51, no. 1 (2018): e62. http://dx.doi.org/10.1002/cpmc.62.

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46

Sakat, J., M. Neou, S. Diry, et al. "Caractérisation de la méthylation de l’ADN des corticosurrénalomes par digital-droplet-PCR." Annales d'Endocrinologie 78, no. 4 (2017): 267. http://dx.doi.org/10.1016/j.ando.2017.07.148.

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47

Malic, Lidija, Jamal Daoud, Matthias Geissler, et al. "Epigenetic subtyping of white blood cells using a thermoplastic elastomer-based microfluidic emulsification device for multiplexed, methylation-specific digital droplet PCR." Analyst 144, no. 22 (2019): 6541–53. http://dx.doi.org/10.1039/c9an01316d.

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Digital droplet PCR for epigenetic leukocyte subtyping from clinically relevant samples is implemented using a thermoplastic elastomer microfluidic droplet generator as a first step towards an economical, customizable and easily deployable system.

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48

Enin, Yaroslav S., Oleg I. Kit, Yuriy A. Gevorkyan, et al. "Detection of KRAS mutations in colon adenocarcinoma by droplet digital PCR." Journal of Clinical Oncology 37, no. 15_suppl (2019): e15080-e15080. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e15080.

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e15080 Background: The screening of mutations in the KRAS gene is a traditional marker of the effectiveness of targeted therapy for colorectal cancer. Mutations in the 12 codon of the second exon of the KRAS gene are present in 40% of colorectal tumors, and monitoring of the mutational status together with the level of mutant DNA is of particular clinical interest. However, the sensitivity level of the traditionally used real-time polymerase chain reaction (RT-PCR) method is insufficient in some cases. Recently, Droplet Digital PCR (DD-PCR) has been considered as an alternative method, which i

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49

Yun, Hyunjin, Jun Ho Ko, Donghwa Lee, et al. "Development of multiplex digital PCR based droplex NSCLC panel test for detecting major mutations in patients with non-small cell lung cancer (NSCLC)." Journal of Clinical Oncology 42, no. 23_suppl (2024): 46. http://dx.doi.org/10.1200/jco.2024.42.23_suppl.46.

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46 Background: Recently, in the treatment of non-small cell lung cancer(NSCLC) patient, immunotherapies and targeted therapies aimed at various genes have been developed. Next-Generation Sequencing(NGS) technology is currently known as the only method for detecting genetic alterations in many different genes simultaneously. However, this technology requires a substantial amount of DNA or RNA input from patients to obtain high-quality results in a clinical setting. We have developed a Droplex NSCLC Panel Test Kit based on multiplex digital PCR, which is known as ideal molecular diagnostic techn

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50

Macagno, Marco, Valeria Pessei, Noemi Congiusta, et al. "A Comparative Study of Methyl-BEAMing and Droplet Digital PCR for MGMT Gene Promoter Hypermethylation Detection." Diagnostics 14, no. 22 (2024): 2467. http://dx.doi.org/10.3390/diagnostics14222467.

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Background: O-6-methylguanine-DNA methyltransferase is responsible for the direct repair of O6-methylguanine lesions induced by alkylating agents, including temozolomide. O-6-methylguanine-DNA methyltransferase promoter hypermethylation is a well-established biomarker for temozolomide response in glioblastoma patients, also correlated with therapeutic response in colorectal cancer. Objectives: The ARETHUSA clinical trial aims to stratify colorectal cancer patients based on their mismatch repair status. Mismatch repair-deficient patients are eligible for treatment with immune checkpoint inhibit

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