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Relevant bibliographies by topics / Digoxigenina

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Academic literature on the topic 'Digoxigenina'

Author: Grafiati

Published: 9 September 2021

Last updated: 29 July 2025

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Journal articles on the topic "Digoxigenina"

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Moreira, Andreia E., José O. Gaspar, and Hugo Kuniyuki. "Detecção do Grapevine virus A e Grapevine virus B por hibridização "dot-blot" com sondas moleculares não radioativas." Fitopatologia Brasileira 30, no. 5 (2005): 538–42. http://dx.doi.org/10.1590/s0100-41582005000500015.

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O vírus A da videira (Grapevine virus A, GVA) e o vírus B da videira (Grapevirus virus B, GVB) estão associados à acanaladura do lenho de Kober ("Kober stem grooving") e ao fendilhamento cortical da videira ("grapevine corky bark"), respectivamente. Este trabalho descreve o uso de sondas moleculares de cDNA na detecção de isolados do GVA (GVA-SP) e do GVB (GVB-C-SP e GVB-I-SP) em videiras (Vitis spp.) e fumo (Nicotiana occidentalis). As sondas marcadas com digoxigenina foram produzidas por RT-PCR utilizando oligonucleotídeos específicos para os genes da proteína capsidial. Os RNA totais foram

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Campillo Pedroza, Natalia, Juan Pablo Franco Salazar, and Juan Carlos Gallego Gómez. "Estandarización de un protocolo para Northern blot no radioactivo usado en la detección de pequeños RNA en células Vero." Revista Colombiana de Biotecnología 17, no. 2 (2015): 123–29. http://dx.doi.org/10.15446/rev.colomb.biote.v17n2.48522.

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<p><strong>Título en ingles:</strong> <strong>Standardization of<em> Northern blot </em>non-radioactive protocol used in the detection of small RNAs in Vero line cells</strong></p><p>El interés en la detección, identificación, y caracterización funcional de los pequeños RNAs no codificantes (sRNAs), ha generado la necesidad de optimizar las metodologías comúnmente usadas en su detección, la reacción en cadena de la polimerasa cuantitativa (RT-qPCR) y <em>Northern blot</em>, con el fin de que sean más sensibles y específicas. A p

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Green, Michael R., and Joseph Sambrook. "Digoxigenin." Cold Spring Harbor Protocols 2022, no. 3 (2021): pdb.top100784. http://dx.doi.org/10.1101/pdb.top100784.

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In molecular cloning, digoxigenin is used as a ligand that can be incorporated into DNA and RNA probes and detected after hybridization with an anti-digoxigenin-antibody enzyme conjugate. Methods to label nucleic acids with digoxigenin and to detect digoxigenin-labeled probes are introduced here.

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Oliveira, Gleicy Kelly de, Ludmila Cristina Oliveira, and Giovana Augusta Torres. "Distribuição de sequências subteloméricas no genoma C de Solanum." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (2018): 154. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp154.

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As sequências subteloméricas CL14 e CL34 foram identificadas em Solanum. tuberosum (genoma A) como longos arranjos adjacentes aos telômeros, sendo localizadas exclusivamente na região terminal dos cromossomos. CL14 está presente também em outras espécies do genoma A e também nos genomas B, P, T e E do gênero, enquanto CL34 foi encontrada apenas em espécies do genoma A. Foi mostrado que essas sequências são muito dinâmicas e evoluem rapidamente nos genomas estudados, mas não foi feita ainda a caracterização nos genomas C e D presentes apenas em espécies poliploides. Esse trabalho teve por objet

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McCreery, Tom. "Digoxigenin labeling." Molecular Biotechnology 7, no. 2 (1997): 121–24. http://dx.doi.org/10.1007/bf02761747.

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Oliveira, Palloma Lima de, Helen Maria Duarte do Rêgo Barros, Thaise da Silva Oliveira Costa, et al. "Análise citogenética em Pecari tajacu (Linnaeus, 1758) e Tayassu pecari (Link, 1795) (Cetartiodactyla: Tayassuidae): Ênfase para distribuição dos sítios de DNAr 35S." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (2018): 79. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp79.

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A família Tayassuidae é composta pelas espécies Pecari tajacu, Tayassu pecari e Catagonus wagneri, apresentando distribuição no continente Americano. Conhecidas, respectivamente, como cateto e queixada, Pecari tajacu e T. pecari são onívoros e indicadores ambientais, encontrando-se em declínio populacional devido à perda de habitat, fragmentação e caça. Com o intuito de identificar marcadores cromossômicos que contribuam para o entendimento dos mecanismos de diferenciação cariotípica desses táxons, este trabalho realizou uma análise citogenética comparativa em P. tajacu e T. pecari, mediante à

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Silva, Gonçalo Santos da, and Margarete Magalhães Souza. "Relações genômicas entre os subgêneros de Decaloba (DC.) Rchb. e Passiflora L." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (2018): 231. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp231.

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A GISH (Genomic In Situ Hybridization) pode ser utilizada com sucesso para determinar a origem e entender as relações evolutivas entre diferentes espécies de plantas. Esse trabalho teve como objetivo analisar as relações genômicas entre os subgêneros Decaloba e Passiflora através da GISH. Foram utilizadas oito espécies do subgênero Passiflora: Passiflora alata Curtis (2n = 18), P. cincinnata Mast. (2n = 18), P. coccinea Aubl. (2n = 18), P. edulis Sims (2n = 18), P. foetida L. (2n = 22), P. ligularis Juss. (2n = 18), P. nitida Kunth (2n = 18), P. vitifolia Kunth (2n = 18) e oito espécies do sub

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Lacarelle, B., R. Rahmani, G. Sousa, A. Durand, M. Placidi, and JP Cano. "Metabolism of digoxin, digoxigenin digitoxosides and digoxigenin in human hepatocytes and liver microsomes." Fundamental & Clinical Pharmacology 5, no. 7 (1991): 567–82. http://dx.doi.org/10.1111/j.1472-8206.1991.tb00746.x.

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Akin, A., C. C. Wu, T. L. Lin, and R. W. Keirs. "Chemiluminescent Detection of Infectious Bursal Disease Virus with a PCR-Generated Nonradiolabeled Probe." Journal of Veterinary Diagnostic Investigation 5, no. 2 (1993): 166–73. http://dx.doi.org/10.1177/104063879300500205.

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A polymerase chain reaction (PCR)-generated digoxigenin-labeled nonradioactive oligonucleotide probe was developed and utilized in slot-blot hybridization coupled with chemiluminescence for the detection of infectious bursal disease virus (IBDV). The probe was prepared from the RNA of the standard challenge strain (STC) of IBDV serotype 1 by reverse transcription followed by 2 PCR amplifications with 2 separate sets of primers. RNA of STC viruses prepared from bursae infected with STC viruses was subjected to the first PCR with the outer primers V8 and V9 that amplified a 607-base pair (bp) se

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Höltke, J., R. Seibl, J. Burg, K. Mühlegger, R. Mattes, and C. Kessler. "Non-radioactive HighSens DNA labeling and detection system (Digoxigenin: anti-digoxigenin based ELISA principle)." Fresenius' Zeitschrift für analytische Chemie 330, no. 4-5 (1988): 377–78. http://dx.doi.org/10.1007/bf00469298.

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Dissertations / Theses on the topic "Digoxigenina"

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Schlehuber, Steffen. "Evolutives Protein-Design eines "Anticalins" mit Bindungsspezifität für Digoxigenin." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963716662.

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Zhang, Peizhi. "Bovine microsatellite analysis using digoxigenin labelling /." [S.l : s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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Huth, Christoph [Verfasser]. "Der Einfluss von „Digoxigenin” auf die mechanische Dysfunktion („atriales stunning“) nach Gleichstromschock an humanen Vorhofmyokardstreifen von Patienten mit und ohne Vorhofflimmern / Christoph Huth." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2012. http://d-nb.info/1027307299/34.

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Chocalheiro, Ana Luísa Mateus. "Lab on paper: biossensores colorimétricos de papel para a detecção de membros do complexo Mycobacterium tuberculosis." Master's thesis, 2014. http://hdl.handle.net/10362/13836.

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Este trabalho foi realizado no âmbito do projecto Lab on Paper, desenvolvido no Centro de Investigação de Materiais (CENIMAT) da Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa (FCT - UNL)<br>Um factor importante para a prevenção e tratamento de doenças é um diagnóstico preciso e precoce. Em 2004, a Organização Mundial de Saúde (OMS) estabeleceu directrizes para o desenvolvimento de meios de diagnóstico adequados a países em via de desenvolvimento e situações de escassez de recursos, sumarizadas pela sigla ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and rob

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Schlehuber, Steffen [Verfasser]. "Evolutives Protein-Design eines "Anticalins" mit Bindungsspezifität für Digoxigenin / Steffen Schlehuber." 2001. http://d-nb.info/963716662/34.

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Chen, Qing Nong, and 陳清農. "Detection of vibrio parahaemolyticus by digoxigenin labeling colony hybridization assay and quantitative targets for PCR by enzyme-linked oligonucleotide sandwich assay." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/69180957076770625012.

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Kubitza, Volker. "Einfluss von Digoxigenin-3, 12-dibromacetat auf die Kontraktionskraft und ⁸⁶Rb⁺(K⁺)-Aufnahme isolierter Herzmuskelpräparate sowie auf die myokardiale Na⁺-K⁺-ATPase des Meerschweinchens." 1987. http://catalog.hathitrust.org/api/volumes/oclc/17426955.html.

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Book chapters on the topic "Digoxigenina"

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Kessler, Christoph, Hans-Joachim Höltke, Rudolf Seibl, et al. "The Digoxigenin: Anti-Digoxigenin (DIG) System." In Nonradioactive Labeling and Detection of Biomolecules. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-662-00144-8_3.

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Kessler, Christoph. "Overview on the Digoxigenin: Anti-Digoxigenin (DIG) System." In Nonradioactive Analysis of Biomolecules. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57206-7_3.

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Wisdom, G. Brian. "Digoxigenin Labeling of IgG Antibody." In Springer Protocols Handbooks. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_67.

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Kösel, Siegfried, Christoph B. Lücking, Rupert Egensperger, and Manuel B. Graeber. "Nonradioactive PCR Sequencing Using Digoxigenin." In PCR Protocols. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_51.

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Arunachalam, Karuppusamy, and Puthanpura Sasidharan Sreeja. "Synthesis of RNA–Digoxigenin Probe." In Springer Protocols Handbooks. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4518-5_49.

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Gathof, Birgit S., and Jan Geißler. "Digoxigenine Labeled Sequencing of the HPRT Gene." In Advances in Forensic Haemogenetics. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78782-9_116.

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Traylor-Knowles, Nikki, and Madison Emery. "Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_19.

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AbstractScleractinians, or stony corals, are colonial animals that possess a high regenerative capacity and a highly diverse innate immune system. As such they present the opportunity to investigate the interconnection between regeneration and immunity in a colonial animal. Understanding the relationship between regeneration and immunity in stony corals is of further interest as it has major implications for coral reef health. One method for understanding the role of innate immunity in scleractinian regeneration is in situ hybridization using RNA probes. Here we describe a protocol for in situ

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Dimo-Simonin, N., C. Brandt-Casadevall, and H. R. Gujer. "Digoxigenin-DNA Probes for Detecting Human VNTR Polymorphism." In DNA — Technology and Its Forensic Application. Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76632-9_18.

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Terenghi, Giorgio. "Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes." In RNA Isolation and Characterization Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-494-1:137.

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Tomassi, Ariel H., Delfina Gagliardi, Damian A. Cambiagno, and Pablo A. Manavella. "Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7165-7_14.

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Conference papers on the topic "Digoxigenina"

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Chen, Hong, Assem Abolmaaty, Peng Li, Constantine Anagnostopoulos, Stefan Du¨bel, and Mohammad Faghri. "Heterogeneous Detection of PCR-Amplified Intimin Gene From E. Coli O157:H7 via PDMS Microfluidic Chip." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11796.

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E. coli O157:H7 strains represent the most important group of food-borne pathogens. PCR-amplified intimin gene of pathogenic E. coli O157:H7 was detected heterogeneously via a microfluidic chip that consists of streptavidin-coated nanoliter chambers. Biotinylated primers and digoxigenin labeled deoxyuridine triphosphate (dUTP) were incorporated into the amplified intimin (eaeA) gene by an off-chip PCR thermal cycler. The amplified products were injected into the chip where they were immobilized via streptavidin-biotin interaction. Detection of the products using alkaline phosphatase (AP) conju

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Liu, Zengbing, Hua Sun, Yu Zheng, Chaojun He, Yuwan Fu, and Min Wang. "Biotransformation of digoxigenin by Arthrobacter simplex TCCC 11037." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639441.

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He, Chaojun, Jingyan Niu, Yumin Yang, and Zengbing Liu. "Novel Biotransformation of Digoxigenin by Colletotrichum lini AS3. 4486." In First International Conference on Information Sciences, Machinery, Materials and Energy. Atlantis Press, 2015. http://dx.doi.org/10.2991/icismme-15.2015.91.

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Vargas, Joseph, David Tacha, Sara Figueroa, and Cristin Douglas. "Abstract 3860: Rodent multispecies multiplex immunohistochemistry using digoxigenin and polymer detection methods in mouse tissues." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-3860.

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Vargas, Joseph, David Tacha, and Sara Figueroa. "Abstract 3831: The development of an immunohistochemical digoxigenin-labeled mouse on mouse single and double stain methodology." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-3831.

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Reports on the topic "Digoxigenina"

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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of

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Jordan, Ramon L., Abed Gera, Hei-Ti Hsu, Andre Franck, and Gad Loebenstein. Detection and Diagnosis of Virus Diseases of Pelargonium. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568793.bard.

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Pelargonium (Geranium) is the number one pot plant in many areas of the United States and Europe. Israel and the U.S. send to Europe rooted cuttings, foundation stocks and finished plants to supply a certain share of the market. Geraniums are propagated mainly vegetatively from cuttings. Consequently, viral diseases have been and remain a major threat to the production and quality of the crop. Among the viruses isolated from naturally infected geraniums, 11 are not specific to Pelargonium and occur in other crops while 6 other viruses seem to be limited to geranium. However, several of these v

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