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Relevant bibliographies by topics / Dinitrosalicylic acid method

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Academic literature on the topic 'Dinitrosalicylic acid method'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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Journal articles on the topic "Dinitrosalicylic acid method"

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Xu, Hui, Yanjun Tian, Kunfu Zhu, et al. "A New Quantitative Determination Method of Acetoin." International Journal of Current Microbiology and Applied Sciences 11, no. 3 (2022): 80–85. http://dx.doi.org/10.20546/ijcmas.2022.1103.010.

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To obtain a new quantitative determination method of Acetoin for reducing the cost of testing and the workload in the process of strain breeding. In an alkaline environment, using 3,5-dinitrosalicylic acid with strong oxidizing properties to azeotrope with Acetoin, 3,5-dinitrosalicylic acid is reduced to 3-amino-5-nitrosalicylic acid and 3-amino-5-nitrosalicylic acid are red-brown substances. The content of Acetoin can be obtained by detecting the absorbance at 540 nm. This new quantitative determination method of Acetoin is a simple, rapid and low-cost method for quantitative determination of

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Gonçalves, Cristiana, Rosa Maria Rodriguez-Jasso, Nelma Gomes, José A. Teixeira, and Isabel Belo. "Adaptation of dinitrosalicylic acid method to microtiter plates." Analytical Methods 2, no. 12 (2010): 2046. http://dx.doi.org/10.1039/c0ay00525h.

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Xia, Meng-lei, Lan Wang, Zhi-xia Yang, and Hong-zhang Chen. "A novel digital color analysis method for rapid glucose detection." Analytical Methods 7, no. 16 (2015): 6654–63. http://dx.doi.org/10.1039/c5ay01233c.

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Wang, Guangyi, Themis J. Michailides, and Richard M. Bostock. "Improved Detection of Polygalacturonase Activity due to Mucor piriformis with a Modified Dinitrosalicylic Acid Reagent." Phytopathology® 87, no. 2 (1997): 161–63. http://dx.doi.org/10.1094/phyto.1997.87.2.161.

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An assay for determination of galacturonic acid with 3,5-dinitrosalicylic acid was developed that substantially extends the linear range of detection compared to a previously published method with this reagent. In the improved assay, galacturonic acid was detected with a reagent containing 44 mM 3,5-dinitrosalicylic acid, 4 mM sodium sulfite, and 375 mM sodium hydroxide. The absorbance of the solution after reaction with galacturonic acid was determined at 575 nm and was linear at concentrations of galacturonic acid up to 50 μmol, with a lower limit of detection at ~400 nmol. The assay with th

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García, Begoña, Inmaculada Domingo, Pedro L. Domingo, and José M. Leal. "Determination of limiting molar conductivities of weak organic acids in aqueous solutions." Collection of Czechoslovak Chemical Communications 56, no. 6 (1991): 1184–92. http://dx.doi.org/10.1135/cccc19911184.

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Conductance, potentiometric, and spectrophotometric measurements have been made at 25°C of aqueous solution containing sulfanilic, hippuric, mandelic, and 3,5-dinitrosalicylic acids at different concentrations. Conductance data were analyzed using the methods of Fuoss-Kraus, Kraus-Parker, and Shedlovsky for conductivity of the free ions. Using the calculated limiting conductances, activity coefficients, and degrees of dissociation, the dissociation constants of the weak acids were determined. From plots Δ vs c1/2, reliable values for Δ0 were estimated, using a computer program, and by means of

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Božinović, Marko, Tea Sokač, Anita Šalić, et al. "Standardization of 3,5-dinitrosalicylic acid (DNS) assay for measuring xylanase activity." Croatian journal of food science and technology 15, no. 2 (2023): 151–62. http://dx.doi.org/10.17508/cjfst.2023.15.2.03.

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The 3,5-dinitrosalicylic acid (DNS) assay has been used for many years mainly to determine the enzymatic activity of xylanase. The assay is based on the detection of reduced sugars. Although the method is widely used, several recent studies have questioned the accuracy of the method. They mainly focused on the detection of side reactions that could lead to a false positive result of the assay. In this study, the basic components of the DNS assay such as buffer preparation, substrate source and concentration, incubation time, reagent preparation, and activity calculation were re-evaluated. Pote

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Simon, P. W., and R. E. Freeman. "A Rapid Method for Screening Reducing Sugar in Carrot Roots." HortScience 20, no. 1 (1985): 133–34. http://dx.doi.org/10.21273/hortsci.20.1.133.

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Abstract Eighteen methods were evaluated to quantify reducing sugars in carrot (Daucus carota L.) roots using drops of juice spotted onto chromatography paper. Reducing sugar detection with dinitrosalicylic acid was rapid (12 to 30 samples per hr) and correlated very well with values obtained by use of high performance liquid chromatography (r = 0.88 overall, ranging from 0.76 to 0.98 in a genetically diverse range of populations). High reducing sugar, low sucrose (Rs/—) roots could be distinguished from low reducing sugar, high sucrose (rs/rs) roots with little error. This method readily comp

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Negrulescu, Anamaria, Viorica Patrulea, Manuela M. Mincea, Cosmin Ionascu, Beatrice A. Vlad-Oros, and Vasile Ostafe. "Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating." Journal of the Brazilian Chemical Society 23, no. 12 (2012): 2176–82. http://dx.doi.org/10.1590/s0103-50532013005000003.

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Lam, Hoa-Hung, Thi-My-Thuong Nguyen, Thi-An-Sa Do, Tuan-Hoang Dinh, and Trung Dang-Bao. "Quantification of total sugars and reducing sugars of dragon fruit-derived sugar-samples by UV-Vis spectrophotometric method." IOP Conference Series: Earth and Environmental Science 947, no. 1 (2021): 012041. http://dx.doi.org/10.1088/1755-1315/947/1/012041.

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Abstract In the present work, the phenol-sulfuric acid method and the 3,5-dinitrosalicylic acid (DNS) method were developed with the aim to quantitatively analyze total sugars and reducing sugars, respectively. In regard with the phenol-sulfuric acid assay, 1.0 mL of sample was treated with 1.0 mL of 5% phenol, 5.0 mL of concentrated H2SO4 and measured at 485 nm, with the linearity range of 10–100 ppm for total sugars. The DNS method was performed on 2.0 mL of sample, using 1.5 mL of DNS at 80 °C for 10 minutes and measured at 510 nm, with the linearity range of 50–400 ppm for reducing sugars.

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B., Marichamy, and Pavithra M. "Complexation of Co(II) with 3,5-dinitrosalicylic acid: Synthesis, Spectral studies and Antibacterial activities." Chemistry Research Journal 8, no. 5 (2023): 1–7. https://doi.org/10.5281/zenodo.11381079.

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<strong>Abstract </strong> The metal complexes have been characterized by elemental analysis, electrical conductance and spectral studies. UV spectra of the complex shows intraligand and charge transfer transitions. Bonding of the metal ion through N- and O- donor atoms of the ligands is revealed by IR studies, and the chemical environment of the protons is confirmed by NMR studies. The agar cup method has been used to study the antibacterial activity of the complexes against the pathogenic bacteria E.Coli, S.aureus and Klebsiella pneumonia.

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Book chapters on the topic "Dinitrosalicylic acid method"

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Jain, Aakanchha, Richa Jain, and Sourabh Jain. "Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosalicylic Acid (DNSA Method)." In Basic Techniques in Biochemistry, Microbiology and Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-4939-9861-6_43.

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Li, He, and Lauren S. McKee. "Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3151-5_2.

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McKee, Lauren S. "Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6899-2_3.

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Sarkar, Mrittika, Anshika Sah, Payal Agarwal, Sai Joshi, and Sinchan Hait. "Quantification Tests for Carbohydrate Detection." In Practical Biochemistry. BENTHAM SCIENCE PUBLISHERS, 2024. http://dx.doi.org/10.2174/9789815165852124010006.

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Quantification of carbohydrates by the anthrone method is a colourimetric assay. In this method, first, the complex carbohydrates are hydrolysed in a highly acidic medium, resulting in monosaccharides. Thereafter, these monosaccharides are dehydrated to form 5-hydroxymethylfurfural (5-HMF) or 2-furfural (2-F), followed by reacting with anthrone reagent. This reaction produces a blue-green complex which is colourimetrically determined. In DNSA method 3,5-dinitrosalicylic acid (DNSA), a yellow colour reagent reacts with reducing sugar’s carbonyl group and forms orangered colour compound 3-Amino-5-nitrosalicylic acid (ANSA). The quantity of carbohydrate present in a sample is determined by Beer-Lambert’s law. Apart from these techniques Folin-Wu, Hexokinase, Phenol-Sulphuric acid, Somogvi-Nelson, and GOPOD method are discussed in this chapter in detail.

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Aruna, B., and Aarthi Kunwar Rathore. "ANTIBACTERIAL EFFECT OF TANNASE PRODUCING BACTERIA - ISOLATED FROM GOAT FAECAL SAMPLE." In Futuristic Trends in Agriculture Engineering & Food Sciences Volume 3 Book 16. Iterative International Publisher, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bcag16p1ch1.

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The faecal samples of goat were used to isolate tannase producing bacteria.The tannase producing isolates (T1 and T2) were identified as Corynebacterium xerosis and Coryne bacterium kutser. The tannase activity found to be,15.2 U/mL (T1 isolate) and 16.5 U/mL (T2) at 37oC; 16.2 U/mL (T1) and 16.1 U/mL (T2) at pH 8by using tannin as asubstrate.The tannase activity was maximum at 37oC and pH 8. The activity of Tannase of the isolates was confirmed by detection of gallic acidby TLC (Thin layer chromatography) and glucose by DNS (3, 5-Dinitrosalicylic acid) method. The T2 Tannase showed antibacterial activity with gram-positive and gram-negative bacteria. Tannins are found in industrial effluents that are toxic to the plant. Tannase producing bacteria that degrade tannins, reduce the toxic effect of tannins towardsplants in contaminated environments and help in plant growth.

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Conference papers on the topic "Dinitrosalicylic acid method"

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Schechner, Pinchas, Eugenia Bubis, and Lea Mor. "Glucose Fueled Alkaline Fuel Cell." In ASME 2005 3rd International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2005. http://dx.doi.org/10.1115/fuelcell2005-74029.

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The electrical behavior of a room temperature membraneless Alkaline Fuel Cell, with incorporated platinum particles in the anode and fueled with glucose, is reported. The Open Circuit Voltage, at different initial glucose concentrations was measured. Changes on the glucose concentration with time were monitored with the dinitrosalicylic acid method. Qualitative Thin Layer Chromatography demonstrates that the electrodes induce polymerization of the glucose. The electrical yield of the system was measured with different external resistors. The Open Circuit Voltage reaches a saturation value of 0

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