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Journal articles on the topic 'Dinitrosalicylic acid method'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Xu, Hui, Yanjun Tian, Kunfu Zhu, et al. "A New Quantitative Determination Method of Acetoin." International Journal of Current Microbiology and Applied Sciences 11, no. 3 (2022): 80–85. http://dx.doi.org/10.20546/ijcmas.2022.1103.010.

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To obtain a new quantitative determination method of Acetoin for reducing the cost of testing and the workload in the process of strain breeding. In an alkaline environment, using 3,5-dinitrosalicylic acid with strong oxidizing properties to azeotrope with Acetoin, 3,5-dinitrosalicylic acid is reduced to 3-amino-5-nitrosalicylic acid and 3-amino-5-nitrosalicylic acid are red-brown substances. The content of Acetoin can be obtained by detecting the absorbance at 540 nm. This new quantitative determination method of Acetoin is a simple, rapid and low-cost method for quantitative determination of

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Gonçalves, Cristiana, Rosa Maria Rodriguez-Jasso, Nelma Gomes, José A. Teixeira, and Isabel Belo. "Adaptation of dinitrosalicylic acid method to microtiter plates." Analytical Methods 2, no. 12 (2010): 2046. http://dx.doi.org/10.1039/c0ay00525h.

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Xia, Meng-lei, Lan Wang, Zhi-xia Yang, and Hong-zhang Chen. "A novel digital color analysis method for rapid glucose detection." Analytical Methods 7, no. 16 (2015): 6654–63. http://dx.doi.org/10.1039/c5ay01233c.

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4

Wang, Guangyi, Themis J. Michailides, and Richard M. Bostock. "Improved Detection of Polygalacturonase Activity due to Mucor piriformis with a Modified Dinitrosalicylic Acid Reagent." Phytopathology® 87, no. 2 (1997): 161–63. http://dx.doi.org/10.1094/phyto.1997.87.2.161.

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An assay for determination of galacturonic acid with 3,5-dinitrosalicylic acid was developed that substantially extends the linear range of detection compared to a previously published method with this reagent. In the improved assay, galacturonic acid was detected with a reagent containing 44 mM 3,5-dinitrosalicylic acid, 4 mM sodium sulfite, and 375 mM sodium hydroxide. The absorbance of the solution after reaction with galacturonic acid was determined at 575 nm and was linear at concentrations of galacturonic acid up to 50 μmol, with a lower limit of detection at ~400 nmol. The assay with th

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5

García, Begoña, Inmaculada Domingo, Pedro L. Domingo, and José M. Leal. "Determination of limiting molar conductivities of weak organic acids in aqueous solutions." Collection of Czechoslovak Chemical Communications 56, no. 6 (1991): 1184–92. http://dx.doi.org/10.1135/cccc19911184.

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Conductance, potentiometric, and spectrophotometric measurements have been made at 25°C of aqueous solution containing sulfanilic, hippuric, mandelic, and 3,5-dinitrosalicylic acids at different concentrations. Conductance data were analyzed using the methods of Fuoss-Kraus, Kraus-Parker, and Shedlovsky for conductivity of the free ions. Using the calculated limiting conductances, activity coefficients, and degrees of dissociation, the dissociation constants of the weak acids were determined. From plots Δ vs c1/2, reliable values for Δ0 were estimated, using a computer program, and by means of

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Božinović, Marko, Tea Sokač, Anita Šalić, et al. "Standardization of 3,5-dinitrosalicylic acid (DNS) assay for measuring xylanase activity." Croatian journal of food science and technology 15, no. 2 (2023): 151–62. http://dx.doi.org/10.17508/cjfst.2023.15.2.03.

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The 3,5-dinitrosalicylic acid (DNS) assay has been used for many years mainly to determine the enzymatic activity of xylanase. The assay is based on the detection of reduced sugars. Although the method is widely used, several recent studies have questioned the accuracy of the method. They mainly focused on the detection of side reactions that could lead to a false positive result of the assay. In this study, the basic components of the DNS assay such as buffer preparation, substrate source and concentration, incubation time, reagent preparation, and activity calculation were re-evaluated. Pote

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Simon, P. W., and R. E. Freeman. "A Rapid Method for Screening Reducing Sugar in Carrot Roots." HortScience 20, no. 1 (1985): 133–34. http://dx.doi.org/10.21273/hortsci.20.1.133.

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Abstract Eighteen methods were evaluated to quantify reducing sugars in carrot (Daucus carota L.) roots using drops of juice spotted onto chromatography paper. Reducing sugar detection with dinitrosalicylic acid was rapid (12 to 30 samples per hr) and correlated very well with values obtained by use of high performance liquid chromatography (r = 0.88 overall, ranging from 0.76 to 0.98 in a genetically diverse range of populations). High reducing sugar, low sucrose (Rs/—) roots could be distinguished from low reducing sugar, high sucrose (rs/rs) roots with little error. This method readily comp

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Negrulescu, Anamaria, Viorica Patrulea, Manuela M. Mincea, Cosmin Ionascu, Beatrice A. Vlad-Oros, and Vasile Ostafe. "Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating." Journal of the Brazilian Chemical Society 23, no. 12 (2012): 2176–82. http://dx.doi.org/10.1590/s0103-50532013005000003.

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Lam, Hoa-Hung, Thi-My-Thuong Nguyen, Thi-An-Sa Do, Tuan-Hoang Dinh, and Trung Dang-Bao. "Quantification of total sugars and reducing sugars of dragon fruit-derived sugar-samples by UV-Vis spectrophotometric method." IOP Conference Series: Earth and Environmental Science 947, no. 1 (2021): 012041. http://dx.doi.org/10.1088/1755-1315/947/1/012041.

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Abstract In the present work, the phenol-sulfuric acid method and the 3,5-dinitrosalicylic acid (DNS) method were developed with the aim to quantitatively analyze total sugars and reducing sugars, respectively. In regard with the phenol-sulfuric acid assay, 1.0 mL of sample was treated with 1.0 mL of 5% phenol, 5.0 mL of concentrated H2SO4 and measured at 485 nm, with the linearity range of 10–100 ppm for total sugars. The DNS method was performed on 2.0 mL of sample, using 1.5 mL of DNS at 80 °C for 10 minutes and measured at 510 nm, with the linearity range of 50–400 ppm for reducing sugars.

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10

B., Marichamy, and Pavithra M. "Complexation of Co(II) with 3,5-dinitrosalicylic acid: Synthesis, Spectral studies and Antibacterial activities." Chemistry Research Journal 8, no. 5 (2023): 1–7. https://doi.org/10.5281/zenodo.11381079.

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<strong>Abstract </strong> The metal complexes have been characterized by elemental analysis, electrical conductance and spectral studies. UV spectra of the complex shows intraligand and charge transfer transitions. Bonding of the metal ion through N- and O- donor atoms of the ligands is revealed by IR studies, and the chemical environment of the protons is confirmed by NMR studies. The agar cup method has been used to study the antibacterial activity of the complexes against the pathogenic bacteria E.Coli, S.aureus and Klebsiella pneumonia.

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11

Kirti, Rani. "Aqueous Two Phase Purification of Vigna radiata Amylase and its Characterization." International Journal of Current Pharmaceutical Review and Research 3, no. 3 (2012): 47–53. https://doi.org/10.5281/zenodo.12697901.

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Amylase is digested the starch from the non-reducing end and thus producing limit dextrinsand maltose too which has industrial applications such as in food, pharmaceutical, paper,leather, detergent and textile industries. Amylase was extracted and purified from Vignaradiata (moong seeds) by a streamline method without the use of proteolytic and lipolyticenzymes. The study features of present method were partial purification of crude enzymeextract done by ammonium sulphate precipitation at low temperature followed by aqueoustwo phase extraction by potassium phosphate-bi-phospahte buffer system

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Mathew, Lata, M. L. P. Reddy, T. Prasada Rao, C. S. P. Iyer, and A. D. Damodaran. "Sensitive flotation-spectrophotometric method for the determination of vanadium (IV) with 3,5-dinitrosalicylic acid and rhodamine B." Fresenius' Journal of Analytical Chemistry 350, no. 3 (1994): 180–81. http://dx.doi.org/10.1007/bf00323184.

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Dian Halimah Batubara, Taslim, Seri Maulina, and Iriany. "HIDROLISIS SELULOSA MENGGUNAKAN KATALIS KARBON TERSULFONASI BERBASIS CANGKANG KEMIRI." Jurnal Teknik Kimia USU 7, no. 2 (2018): 23–27. http://dx.doi.org/10.32734/jtk.v7i2.1645.

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 Cogon grass (Imperata cylindrica) contains cellulose which is convertible to glucose through hydrolysis by using inorganic liquid acid catalyst. However, the use of such catalyst leads to corrosion problem, environment pollution, and complex separation. To overcome this problem, a sulfonated carbon catalyst was proposed. This study aimed to evaluate candlenut shell as carbon source for catalyst support in sulfonated carbon catalyst, and its application in cellulose hydrolysis. Candlenut shell was carbonized at 300-550oC for 4 h. Resulting carbon was sulfonated at 120-150oC for 6 h. Sulf

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Li, Qing, Youmin Jiang, Changpeng Ren, et al. "Effects of Different Hydrolysis Methods on the Hydrolysate Characteristics and Photo-Fermentative Hydrogen Production Performance of Corn and Sorghum Straw." Energies 16, no. 1 (2022): 301. http://dx.doi.org/10.3390/en16010301.

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The effects of hydrolysis methods (hydrothermal, acid, alkali, hydrothermal-enzyme, acid-enzyme, and alkali-enzyme) on hydrolysate characteristics and photo fermentative hydrogen production (PFHP) of corn straw (CS) and sorghum straw (SS) were investigated. The optimum production of reducing the sugar of straw in different solvent environments was studied by one-step hydrolysis and co-enzymatic hydrolysis pretreatment through a 3,5-dinitrosalicylic acid method. The hydrogen production process by photolytic fermentation of hydrolysates of Rhodobacter sphaeroides HY01 was further analyzed throug

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NOOR MOHAMAD ZIN, NOOR SYAFFINAZ, NUR AIN SABRINA AZMI, NURSARAH SYAMIMI ANUAR, et al. "A 96-WELL-PLATE–BASED METHOD FOR THE ESTIMATION OF ALPHA-AMYLASE ACTIVITY USING MINIATURISES 3,5-DINITROSALICYLIC ACID (DNSA) COLORIMETRIC METHOD." Malaysian Applied Biology 51, no. 4 (2022): 95–102. http://dx.doi.org/10.55230/mabjournal.v51i4.16.

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The DNSA assay has been widely employed for the in vitro detection and quantification of alpha-amylase inhibitory activity. However, the conventional method is associated with inconsistencies between protocols and requires a large volume of samples and other assay reagents that can compromise accurate quantitation. Therefore, the study aimed to develop a reliable, simple, and rapid analytical method for determining α-amylase activity. The developed method was carried out in 96-well microplates with a total volume of 250 µL and a total assay time of 1 hr, including pre-incubation. The method wa

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Taslim, Dian Halimah Batubara, Seri Maulina, Iriany, and Okta Bani. "Preparation and Characterization of Sulfonated Carbon from Candlenut Shell as Catalyst for Hydrolysis of Cogon Grass Cellulose into Glucose." Asian Journal of Chemistry 32, no. 6 (2020): 1404–8. http://dx.doi.org/10.14233/ajchem.2020.22613.

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Cogon grass (Imperata cylindrica) is convertible into glucose by hydrolysis process, which usually requires a catalyst. A solid acid catalyst of sulfonated carbon was used in this work. This study aimed to observe the viability of candlenut shell as carbonaceous source in solid acid catalyst production and to characterize the sulfonated carbon. The carbonization was performed at 250-550 ºC for 4 h, while sulfonation was carried out at 100-180 ºC for 6 h. Sulfonated carbon was then characterized by H+ activity/acid density test, scanning electron microscopy-energy dispersive X-ray spectroscopy

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Novik, Nurhidayat, Sudaryati Soeka Yati, Suciatmih ., Triana Evi, and Yulinery Titin. "Cellulase Activity in the Growing Media and Physicochemical Content of the Auricularia auricula -Judae Fruiting Body." Research Journal of Biotechnology 19, no. 9 (2024): 1–10. http://dx.doi.org/10.25303/1909rjbt01010.

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This study examined the cellulase activity from Auricularia auricula-judae planting media before harvest and the physicochemical content of the A. auricula-judae fruiting body. Cellulase enzyme activity was determined by calculating the reducing sugars of the medium using the 3,5-dinitrosalicylic acid method. The optimum activities of cellulase enzymes and reducing sugar content in the second harvest were 1.63 (U/mL) and 22.39 (mg/mL) at pH 8.0 respectively. The physicochemical results of the fruiting body at the 2nd harvest proximate (%) levels of water, ash, fat, protein, carbohydrates, crud

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CHEN, RUI, JIAXUAN LI, ZHIWEI YANG, et al. "Determination of 3,5-Dinitrosalicylic Acid Hydrazide in Honey by Solid-Phase Extraction–Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry." Journal of Food Protection 83, no. 5 (2020): 910–14. http://dx.doi.org/10.4315/jfp-19-532.

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ABSTRACT 3,5-Dinitrosalicylic acid hydrazide (DNSAH) is the metabolite of the antibacterial nitrofuran nifursol. A simple and accurate analytical method to determine DNSAH levels in honey by solid-phase extraction–ultraperformance liquid chromatography–tandem mass spectrometry has been established. The honey sample was hydrolyzed under acidic conditions and derivatized with 2-nitrobenzaldehyde in the dark for 16 h, followed by solid-phase extraction and column chromatography. Detection was performed with an electrospray ionization source and multiple reaction monitoring mode and was quantified

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Lempiäinen, Henna, Katja Lappalainen, Jasmiina Haverinen, et al. "The Effect of Mechanocatalytic Pretreatment on the Structure and Depolymerization of Willow." Catalysts 10, no. 2 (2020): 255. http://dx.doi.org/10.3390/catal10020255.

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In this study, the effect of a mechanocatalytic pretreatment on the structure of willow and sugar release from pretreated willow was explored. In the mechanocatalytic approach, the pretreatment consists of solvent-free impregnation with sulfuric acid and a mechanical treatment with ball milling. Willow sawdust and pretreated samples were analyzed with field emission scanning electron microscope and X-ray diffraction. The products in the sugar solution were determined as the total reducing sugars with the 3,5-dinitrosalicylic acid method and monosaccharides with capillary electrophoresis. Accor

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Putri Asih Mahalani, Kiki Mulkiya, and Thyazen Abdo Hizam Al-Hakim. "Uji Aktivitas Penghambatan Enzim Alfa Amilase Ekstrak Daun Kelor (Moringa Oleifera)." Bandung Conference Series: Pharmacy 4, no. 2 (2024): 720–27. http://dx.doi.org/10.29313/bcsp.v4i2.14873.

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Abstract. Diabetes mellitus (DM) is a metabolic disorder resulting from loss of tolerance to carbohydrates which is influenced by genetic and clinical factors. Efforts to treat DM include inhibiting α-amylase, a digestive enzyme that breaks down starch in food. Moringa leaves have been used practically in the field of medicine. In vivo and in vitro studies on the Moringa plant show its effectiveness in treating inflammation, hyperlipidemia, and hyperglycemia. Moringa has phytochemical properties including flavonoids and felnolic acid with inflammation-related, antioxidant, and antibacterial ac

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B., S. Adeleke, O. Ojo S., D. Oluwafemi Y., and O. Olaniyi O. "Chemical Mutagenesis of Bacillus subtilis for Improved Mannanase Biosynthesis." Journal of Advances in Microbiology 3, no. 1 (2017): 1–6. https://doi.org/10.9734/JAMB/2017/30911.

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The aim of the present study was to isolate bacterial associated with abattoir waste water, compost saw dust, soil and water samples from Ilaje Lake, Ondo State, Nigeria. The microbial isolates were identified using standard microbiological method. The bacterial isolates were screened for mannanase production. Mannanase activity was determined by dinitrosalicylic acid (DNSA) method while protein concentration in the fermentation broth was quantified by Lowry’s method. Isolate designated 2k tentatively identified as <em>Bacillus subtilis </em>had the highest mannanase activity. The isolate with

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Ojewumi, M. E., O. R. Obanla, O. O. Fagbiele, and O. E. O. Kolawole. "LAND REMEDIATION AND RECLAMATION TECHNIQUES THROUGH THE BIODEGRADATION OF WASTE PAPERS." Journal of Southwest Jiaotong University 57, no. 4 (2022): 28–40. http://dx.doi.org/10.35741/issn.0258-2724.57.4.3.

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Waste materials are one of the major components of solid organic waste that pollutes land. Inappropriate disposal of municipal waste (solid) not only affects land but is also a source of danger to inhabitants. The management of residue from incineration is an integral part of waste disposal/management systems. This research provides a collection and bio-treatment technologies for various types of waste paper such as office paper and newspaper as feedstock using two microorganisms: Serratia and Rhizopus to convert them to reducing sugars. The percentages of reducing sugar present in the samples

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Van Anh, Trinh Thi, Nguyen Quynh Uyen, Nguyen Ngoc Hong, Dinh Thi Lam, and Hoang Van Vinh. "Preparation of fusarium oxysporum-inhibiting chito-oligosaccharide by chitin/chitosan-hydrolyzing enzyme from actinomycetes." Vietnam Journal of Biotechnology 19, no. 4 (2022): 765–70. http://dx.doi.org/10.15625/1811-4989/15633.

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Chito-oligosaccharide (COS), one of remakable derivatives of chitosan, has almost chitosan’s biological function but better activities in antibacteria, antifungi and plant elicitor… Producing COS by biological enzymes shows many advantages in comparision with physical and chemical methods. In our study, COS was prepared using chitin/chitosan-hydrolyzing enzyme from actinomycetes. By analysis of 16S rDNA sequence, the enzyme producer VTCC 940003 belonged to Streptomyces macrosporeus. The strain’s ability to synthesize the enzyme having chitin/chitosan-hydrolyzing activity was first detected by

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Morrison, David, J. Susan van Dyk, and Brett Ivan Pletschke. "The effect of alcohols, lignin and phenolic compounds on the enzyme activity of Clostridium cellulovorans XynA." BioResources 6, no. 3 (2011): 3132–41. http://dx.doi.org/10.15376/biores.6.3.3132-3141.

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In the production of biofuel from lignocellulose biomass, particularly in the case of consolidated bioprocessing where the saccharification and fermentation steps take place within the same bioreactor, many compounds may be present that could affect the enzymes within such a bioreactor. This study examined the effect of ethanol, butanol, propanol, lignin, r-coumaric acid, and gallic acid on the activity of XynA from C. cellulovorans. XynA from C. cellulovorans was purified, and the effects of various compounds on enzyme activity were assayed using the dinitrosalicylic acid method. In this stud

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Sumarno, Sumarno, Yeni Rahmawati, P. N. T. Risanti, and N. E. Mayangsari. "The Effect of Decomposition Time on Cellulose Degradation in Ionic Liquid/Acid with Pressurized CO2." Modern Applied Science 9, no. 7 (2015): 69. http://dx.doi.org/10.5539/mas.v9n7p69.

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Cellulose material is the most abundant carbohydrate that has a simple polymer structure, but it forms ofcrystalline micro-fibrils lead it insoluble in various solvent and highly resistant for hydrolysis process. Thedegradation of cellulose into glucose will increase the raw material for production of ethanol, isopropanol orbutanol. The conversion into oligomer can be applied for pharmaceutical, food additives, etc. There are manytechnologies for conversion of cellulose such as degradation with ionic liquids, acid, enzymatic/fermentation,and hydrothermal. In this work, we studied cellulose dec

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Kiran, Nimra, Fakhar-Un-Nisa Yunus, Muhammad Nadeem, et al. "Potato peel as substrate for Single Cell Protein in animal feed by submerged fermentation." Italian Journal of Food Science 36, no. 4 (2024): 57–63. http://dx.doi.org/10.15586/ijfs.v36i4.2641.

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Single-cell protein (SCP) has gained attention in scientific research as a vital source of protein in animal feed and human food. Taking into account the importance of SCP, an attempt was made in the current research to grow Rhizopus oligosporus and Saccharomyces cerevisiae biomass by selecting potato peel as a substrate. The potato peel was treated with sulfuric acid to form potato peel hydrolysate following acid hydrolysis. In 1.0-L potato peel hydrolysate, 2.5-g/L KH2PO4, 0.5-g/L MgSO4, 0.5-g/L NaCl, and 10 g/L yeast extract were taken to prepare growth media. The moisture content of potato

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Nasution, A. F., E. Munir, D. Suryanto, and Y. Yurnaliza. "Potential of cellulolytic fungi isolated from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros)." IOP Conference Series: Earth and Environmental Science 1115, no. 1 (2022): 012072. http://dx.doi.org/10.1088/1755-1315/1115/1/012072.

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Abstract Cellulose is a potential source of fermentable sugars and a promising cost-effective source for biofuel production. This study aimed to obtain potential cellulolytic fungi from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros L.). The cellulolytic fungi were isolated using Potato Dextrose Agar (PDA) media using spread plate method. Qualitative and quantitative screening tests were carried out using 1% of CMC (w/v) as a sole carbon source. Twelve isolates were recovered with varying cellulolytic capabilities. The highest cellulolytic activity was obtained from KOr-15J wit

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Chasanah, Choirul, та Das Salirawati. "The Influence of Al3+ Metal Ion in AlCl3 Compound On α- Amylase Enzyme Activity". Indonesian Journal of Chemistry and Environment 3, № 2 (2021): 45–52. http://dx.doi.org/10.21831/ijce.v3i2.43512.

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The influence of Al3+ metal ion in AlCl3 towards α-amylase enzyme activity have been studied. The activity of the α-amylase enzyme on potato starch substrate was determined using the DNS method (3,5-dinitrosalicylic acid) at optimum conditions. The data analysis was conducted in a descriptive qualitative manner. The results obtained that α-amylase enzyme activity with potato starch substrate was optimum at 20 mg/mL with pH 7.2, incubation temperature 37˚C, incubation time 15 minutes, and enzyme concentration of 40 mg/mL with an average enzyme activity value of 0.0079 mg/mL/minute at 37˚C. Howe

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Subaryono, Subaryono, Yuwanita Ardilasari, Rosmawaty Peranginangin, Fransisca Rungkat Zakaria, and Maggy Thenawidjaja Suhartono. "Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 11, no. 2 (2017): 45. http://dx.doi.org/10.15578/squalen.v11i2.250.

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Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity. This research was conducted to evaluate activity of the alginate lyase enzyme at various pHs, temperatures and substrates. Polymannuronate and polyguluronate were used to evaluate substrate specificities. Alginate lyase activity was assayed by analysis of reducing sugar released using the 3,5 dinitrosalicylic acid (DNS) method. The research showed that the activity of alginate l

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Mwenje, E., and N. Mguni. "Cellulolytic and pectinolytic activities of Capnodium isolates (sooty mould) from Zimbabwe." Canadian Journal of Botany 79, no. 12 (2001): 1492–95. http://dx.doi.org/10.1139/b01-126.

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The cellulolytic and pectinolytic activities of five Capnodium (sooty mould) isolates previously associated with preharvest spoilage of avocado (Persea americana Mill.) fruits in Zimbabwe were assessed in liquid culture and in artificially infected avocado fruits. Polygalacturonase, endo-1,4-β-glucanase, and exo-1,4-β-glucanase activities were determined by measuring the increase in reducing groups using the dinitrosalicylic acid method, while for pectin lyase activity the thiobarbituric acid method was used. The five isolates showed the ability to produce polygalacturonase, pectin lyase, and

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Rahmatil Majidah, Syafrina Sari Lubis, and Diannita Harahap. "POTENSI ENZIM SELULASE DARI BAKTERI TERMOFILIK DI KAWASAN WISATA IE SUUM KABUPATEN ACEH BESAR." KENANGA : Journal of Biological Sciences and Applied Biology 3, no. 1 (2023): 25–34. http://dx.doi.org/10.22373/kenanga.v3i1.3119.

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Thermophilic bacteria are a group of bacteria that grow at a temperature range of 45oC to 80oC and have the potential to produce cellulase enzymes. The tourist area of Ie Suum, Aceh Besar District is one of the hot springs in Aceh. This study aims to observe the morphological characteristics, cellulase enzyme potential and activity test in producing cellulase enzymes from thermophilic bacteria found in Ie Suum. The method of isolating thermophilic bacteria uses the pour plate method and testing the cellulase enzyme activity using the DNS (3,5-dinitrosalicylic Acid) method. 15 isolates were obt

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Joseph, Shanta, Shailesh Kumar Jadhav, Avdhesh Kumar, Jag narayan, and Suchita Salni Toppo. "Aeromycoflora: A Source of Novel Enzyme of Industrial Importance." International Journal of Current Microbiology and Applied Sciences 13, no. 12 (2024): 155–62. https://doi.org/10.20546/ijcmas.2024.1312.017.

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The present study focuses on the isolation and screening of Aeromycoflora fungi for their potential to produce industrially significant enzymes such as amylase, cellulase, and xylanase. Airborne fungal samples were collected using the gravity plate method on Potato Dextrose Agar (PDA) and incubated under optimal conditions. A total of 10 isolates were obtained and screened qualitatively for enzyme production. The ability of the isolates to produce amylase, cellulase, and xylanase was assessed using starch agar, carboxymethyl cellulose (CMC) agar, and Xylan agar plates, respectively. Zones of h

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BULUT ATALAY, Esra, and Hülya AYAR KAYALI. "Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis." Uluslararası Muhendislik Arastirma ve Gelistirme Dergisi 15, no. 1 (2023): 117–24. http://dx.doi.org/10.29137/umagd.1142303.

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The metabolism is reprogrammed in cancer cells to meet the requirement of their malignant properties. Some specific metabolites can contribute to the malignant transformation in different cancer types. This study aimed to determine the level of glucose, pyruvate, lactate, citrate, alpha-ketoglutarate (α-KG), 2-hydroxyglutarate (2-HG), succinate, fumarate, malate, and oxaloacetate in the different tissues (tumor, colon, brain, and liver) of xenograft models generated by a colon adenocarcinoma cell line (SW480). Glucose and pyruvate levels were determined by the 3,5-dinitrosalicylic acid (DNS) m

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Ganeshprasad, D. N., Yalpi Karthik, H. R. Sachin, and A. H. Sneharani. "Polysaccharide hydrolyzing enzyme activity of bacteria, native to Apis florea gut." Biomedicine 41, no. 4 (2021): 768–75. http://dx.doi.org/10.51248/.v41i4.1013.

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Introduction and Aim: Apis florea commonly known as “dwarf honey bee” harbors enormous gut bacteria that can digest complex carbohydrates and other food components. In this regard, the present investigation was focused on analyzing the polysaccharide degrading ability of bacteria isolated from the gut of honeybee, for their possible application in nutraceutical and pharmaceutical industries. Materials and Methods: Nine bacterial isolates were screened for carbohydrate degrading enzymes viz., amylase, pectinase, cellulase, tannase and laccase, using respective substrate by plate assay method. F

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Rivadeneira, J. P., M. E. Flavier, and F. R. P. Nayve, Jr. "Optimization of Acid and Steam Explosion Pretreatment of Cogon Grass for Improved Cellulose Enzymatic Saccharification." Eurasian Chemico-Technological Journal 21, no. 2 (2019): 143. http://dx.doi.org/10.18321/ectj824.

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Acid-impregnation and its combination with steam explosion were evaluated and optimized using Response Surface Methodology. At 10% solid-liquid ratio, cogon was impregnated with diluted H2SO4 solution (0 to 3%, w/w) at different ranges of temperature (40 to 120 °C) and varied time (0 to 130 min). Impregnated samples were then subjected to enzymatic saccharification using 60 FPU/g Accelerase 1500™. After enzymatic saccharification, the concentration of reducing sugar released was measured using Dinitrosalicylic (DNS) Colorimetric Method. Based on the results, Response Surface Model (RSM) showed

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Jaiswal, Priyanka, and Padma Kumar. "ALPHA AMYLASE INHIBITORY ACTIVITY OF DIFFERENT EXTRACT OF BARK OF ALBIZIA LEBBECK (L.) BENTH." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 8 (2017): 119. http://dx.doi.org/10.22159/ijpps.2017v9i8.19411.

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Objective: To evaluate the alpha amylase inhibitory activity of different extract of bark of Albizia lebbeck (L.) Benth.Methods: Bark of A. lebbeck was collected and air dried. Extraction of flavonoid, alkaloid and steroid carried out using standard methods. Extracts in different polar and non polar solvents were extracted using soxhlet method. Filtered extracts were subsequently tested for alpha amylase inhibitory activity using the chromogenic dinitrosalicylic acid (DNSA) method and starch iodine method. Experiments were performed in triplicates for three different sets and mean±standard err

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Byagsa, Yarisina, Wenny Bekti Sunarharum, Tunjung Mahatmanto, and Yoga Dwi Jatmiko. "Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica)." BIO Web of Conferences 91 (2024): 01024. http://dx.doi.org/10.1051/bioconf/20249101024.

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Liberica coffee has an inferior quality when compared to arabica and robusta coffee. This flavour can be improved through a fermentation process involving indigenous microbes, one of which is a group of pectinolytic yeasts. Therefore, this study aimed to obtain yeast isolates with pectinolytic activity. Green bean samples were used for yeast isolation using YEPD agar media. Selected yeast isolates were screened for their pectinolytic activity semi-quantitatively using the well-diffusion method and quantitatively using the dinitrosalicylic acid (DNS) method. A total of six yeast isolates with d

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Widjaja, Tri, Siti Nurkhamidah, Nuniek Hendrianie, Aisyah Alifatul Zahidah Rohmah, and Kharisma Perdana Setiawan. "Optimization of Acid-Catalyst Hydrolysis Process in Lactic Acid Production from Rice Husk by Using Lactobacillus bacteria." Journal of Hunan University Natural Sciences 49, no. 3 (2022): 141–50. http://dx.doi.org/10.55463/issn.1674-2974.49.3.15.

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Lactic acid is an important component of manufacturing polylactic acid (PLA), which can be produced with high-lignocellulosic biomass, still few of them have utilized that. The production process starts with Alkaline Hydrogen Peroxide (AHP) pretreatment, acid-catalyzed (H2SO4) hydrolysis followed by post-hydrolysis, and fermentation using a mixture of Lactobacillus brevis + Lactobacillus rhamnosus and single Lactobacillus delbrueckii bacteria. The effectiveness of pretreatment results was analyzed by using Thermal Gravity Analysis (TGA), which resulted in the removal of lignin and cellulose ar

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Gawade, Bhimraj, and Mazahar Farooqui. "SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC ACTIVITY OF BAUHINIA RACEMOSA LAM. LEAVES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 6 (2018): 190. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.24038.

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Objective: In this study, the leaves of medicinal plant Bauhinia racemosa Lam. with different pharmacological activities were subjected to phytochemical screening and assessment of their in vitro inhibitory potential with porcine pancreatic α-amylase enzyme to treat and management of diabetes.Methods: Plant leaves were extracted sequentially with ethanol solvent. A modified 3,5-dinitrosalicylic acid method was adopted to screen α-amylase inhibition assay. The ethanol extract was analyzed qualitatively and gas chromatography–mass spectrometry analysis technique for the active phytoconstituents

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Dimawarnita, Firda, Gendis Salsa Andhayu, Yora Faramitha, and Elya Mufidah. "Production of crude enzyme from Trichoderma sp." BIO Web of Conferences 99 (2024): 02004. http://dx.doi.org/10.1051/bioconf/20249902004.

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Cellulose enzymes can degrade and hydrolyze the β-1,4 glycosidic bond in cellulose, resulting in various products, including glucose. This study aimed to find a species of Trichoderma and determine the optimal growth day for producing cellulase enzymes with the highest activity. Trichoderma is a fungus that can produce cellulase enzymes. The study was conducted to test the cellulase enzyme activity produced by a specific species of Trichoderma to identify the species with the highest activity level. A Trichoderma species that can increase and have the best activity in producing cellulase enzym

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Nurvita Abdullah, Netty Ino Ischak, La Alio, Yuszda K. Salimi, La Ode Aman та Ahmad Kadir Kilo. "Inhibis Enzim α-Glukosidase dan α-Amilase dari Ekstrak Metanol Daun Buhu (Garuga floribunda Decne) Sebagai Antidiabetes". Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa 3, № 1 (2024): 114–30. https://doi.org/10.62383/algoritma.v3i1.360.

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The Garuga floribunda (Garuga floribunda Decne) plant is one of the species known for its various medicinal properties. This research aims to investigate the inhibitory activity of α- glucosidase and α-amylase enzymes and to determine the optimum concentration of the methanol extract of Garuga floribunda leaves as an antidiabetic agent. The leaves extraction is obtained through an extraction process using methanol as the solvent and tested for itsinhibitory activity against the α-glucosidase enzyme using the p-nitrophenyl-α-D- glucopyranoside (p-NPG) substrate and the α-amylase enzyme using th

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He, Shuting, Tiantian Lu, Xiaoyu Sun, et al. "Multi-Method Combined Screening of Agarase-Secreting Fungi from Sea Cucumber and Preliminary Analyses on Their Agarases and Agar-Oligosaccharide Products." Microorganisms 13, no. 6 (2025): 1235. https://doi.org/10.3390/microorganisms13061235.

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Agar can be degraded into agar-oligosaccharides by physical, chemical, and biological methods, but the further industrial application of agar-oligosaccharides has been limited by the environmental pollution of traditional agar-oligosaccharides preparation methods and the lack of novel agarase. In this study, we reported the screening of 12 strains with agar-degrading activity from sea cucumber intestine and mucus using a combination of Gram’s iodine staining and 3,5-dinitrosalicylic acid (DNS) method, during which five fungal strains exhibited high agarase activity. Their production of differe

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Wang, Wei Guo, Peng Li, Yu Jia Cui, et al. "Preparation of Grifola frondosa Fermented Beverage without Preservatives." Advanced Materials Research 554-556 (July 2012): 1110–15. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1110.

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Purpose To prepare Grifola frondosa fermented beverage and study on its production process, ingredients and characteristics. Methods The following process was used to prepare Grifola frondosa fermented beverage: Grifola frondosa original strains →Strain activation →Inclined plane strain →Liquid spawn →Submerged fermentation →Fermentation broth with mycelium →Colloid mill grinding →High pressure homogenization →Homogenate →Add preservatives or not →Package → Sterilizing or not →Finished product. Grifola frondosa mycelium biomass was determined by Weight-Drying method. Total sugar content was me

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Kida,, Zulkifli Hussaini. "Production, Characterization and Optimization of Bioethanol from Microalgae Obtained from Waste Water in Maiduguri Metropolitan Council, Borno State- Nigeria." Arid Zone Journal of Basic and Applied Research 2, no. 2 (2023): 10–22. http://dx.doi.org/10.55639/607.191817.

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Bioethanol is a renewable, colorless, less toxic, and readily biodegradable form of fuel from biological sources; that can be used for heat, electricity, and fuel. In this research study, an alternative feedstock known as microalgae was used. Bioethanol was produced from microalgae using enzymatic hydrolysis. The microalgae was pretreated with acid and alkaline. Hydrolysis was carried out by Aspergillus niger and Saccharomyces cerevisiae was used for fermentation. The concentration of the Bioethanol produced was determined using acid Potassium Dichromate. After hydrolysis, the reducing sugar c

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Li, Jiaqing, Zhenwu Wei, Xueyang Min, Peizhou Zhao, Linghua Yang, and Nana Liu. "Physiological and Biochemical Changes in the Seeds of Naturally Aged Wenling Medic (Medicago polymorpha) with Its Recovery of Viability." Agronomy 13, no. 3 (2023): 787. http://dx.doi.org/10.3390/agronomy13030787.

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Wenling Medic (Medicago polymorpha) is common in southern China and has long been utilized as a vegetable in eastern China, as well as a significant raw ingredient for livestock and pickled meals. As a legume, there is still a research vacuum, and the first problem Wenling Medic faces in production is the problem of seed germination. The germination percentage of Wenling Medic was low, according to production practice and laboratory tests performed in this work. Furthermore, after more than two years of storage, the germination percentage of Wenling Medic dropped sharply, and it lacked a long

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Sun, Jing, Hong Yang, Shangjie Ge-Zhang, Yujie Chi, and Dawei Qi. "Identification of a Fomitopsis pinicola from Xiaoxing’an Mountains and Optimization of Cellulase Activity." Forests 15, no. 9 (2024): 1673. http://dx.doi.org/10.3390/f15091673.

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Brown-rot fungi are large fungi that can decompose the cell walls of wood; they are notable for their secretion of diverse and complex enzymes that synergistically hydrolyze natural wood cellulose molecules. Fomitopsis pinicola (F. pinicola) is a brown-rot fungus of interest for its ability to break down the cellulose in wood efficiently. In this study, through a combination of rDNA-ITS analysis and morphological observation, the wood decay pathogen infecting Korean pine (Pinus koraiensis Siebold and Zucc.) was identified. Endoglucanase (CMCase) and β-glucosidase were quantified using the DNS

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Prastiwi, Danty Oktiana, Novia Anggita, and Yudha Putra Arishandy. "Bioethanol Production from Tapioca-Waste as Potential Additive Fuel for LCGC (Low-Cost Green Car)." Current Biochemistry 6, no. 1 (2019): 28–34. http://dx.doi.org/10.29244/cb.6.1.6.

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The majority style of people in transportation is 24% contribute to increasing premium demand as energy. Unfortunately, fossil fuel is not renewable and limited quantity. Its means, potential biomass of tapioca waste should be an alternative energy source to solve that problem. Today, the biosystem of bioethanol production needs to be optimized to maximize filtration and minimize the production cost. First, composition and time incubation needs to be optimized. The research found that to fermented 50 g waste on 200 mL water, we need 2 g of yeast, and the time of harvesting optimum is fourth da

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Bakri, Y., Y. Akeed, M. Jawhar, and M. I. E. Arabi. "EVALUATION OF XYLANASE PRODUCTION FROM FILAMENTOUS FUNGI WITH DIFFERENT LIFESTYLES." Acta Alimentaria 49, no. 2 (2020): 197–203. http://dx.doi.org/10.1556/066.2020.49.2.9.

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Xylanase plays an important role in the food, feed, and pulp/paper industry. Filamentous fungi have been considered as useful producers of this enzyme from an industrial point of view, due to the fact that they excrete xylanases into the medium. In this study, four fungal species belonging to different genera, i.e. Aspergillus, Cochliobolus, Pyrenophora, and Penicillium were isolated from different sources and compared for their ability to produce xylanase in submerged culture. The fungal species showed enzyme activity as determined by dinitrosalicylic acid (DNS) method. It was found that the

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Xie, Sandu, Huiqing Chen, Xinyan Jiang, et al. "Structural and Physicochemical Properties of a Chinese Yam Starch–Tea Polyphenol Complex Prepared Using Autoclave-Assisted Pullulanase Treatment." Foods 12, no. 20 (2023): 3763. http://dx.doi.org/10.3390/foods12203763.

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Interactions between food components have a positive impact in the field of food science. In this study, the effects of tea polyphenol on the structural and physicochemical properties of Chinese yam starch using autoclave-assisted pullulanase treatment were investigated. X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, rapid visco analysis, differential scanning calorimetry, and the 3,5-dinitrosalicylic acid method were applied in this study. The results showed that the Chinese yam starch–tea polyphenol complex formed a structural domain with higher the

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Widyastanti, Sekar, and Trianik Widyaningrum. "Produksi Bioetanol Limbah Nasi Aking Fermentasi Menggunakan Zymomonas mobilis dengan Perlakuan Konsentrasi Crude Enzim Bacillus amyloliquefaciens." Bioscientist : Jurnal Ilmiah Biologi 10, no. 2 (2022): 901. http://dx.doi.org/10.33394/bioscientist.v10i2.6249.

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Bioethanol fuel has the advantage of being more environmentally friendly than petroleum. Bioethanol is made from organic materials containing glucose. Baking rice contains 83.19% (w/w) carbohydrates and 29.70% (w/w) amylose which can be converted into bioethanol through hydrolysis and fermentation stages. This type of research is an experimental research, the initial stage carried out is the hydrolysis stage to obtain reducing sugars using crude Bacillus amyloliquefaciens enzymes with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15 %, and 17.5% for 6 days. The next step is the fermentatio

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