Academic literature on the topic 'Direct bioautography'

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Journal articles on the topic "Direct bioautography"

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Anuar, Nadia, Sadia Sultan, and Kamran Ashraf. "An Overview of Antimicrobial and Antioxidant Bioautography Method Analysis: Cosmos caudatus and Orthosiphon stamineus." International Journal of Pharmaceuticals, Nutraceuticals and Cosmetic Science 5, no. 1 (2022): 1–12. http://dx.doi.org/10.24191/ijpnacs.v5i1.01.

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Medicinal herbs such as Cosmos caudatus and Orthosiphon stamineus are sources of a huge number of bioactive compounds with antioxidant and antimicrobial properties. C. caudatus plant has bioactive compounds such as phenolics, flavonoids, and terpenoids. On the other hand, phytochemicals from O. stamineus have been shown to have a wide range of traditional applications due to their antioxidant and antibacterial characteristics. The bioautography method is used to isolate active molecules on chromatogram followed by the chemical and biological detection system. In antimicrobial bioautography, ag
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Vishwa, Patel1* Niyati Acharya2. "Application of TLC Bioautography for Natural Bioactive Screening." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1376–86. https://doi.org/10.5281/zenodo.15378848.

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Bioautography is a mean of targeted-directed isolation of active molecules on chromatogram. Bioautography is technique to detect substances affecting the growth rates to test organisms in complex mixtures and matrices. The antimicrobial, antioxidant and enzyme inhibitory activities can be performed on TLC bioautography. Bioautography methods are divided into three categories. 1)Agar diffusion or contact bioautography 2) Immersion or agar-overlay bioautography 3) Direct bioautography. High throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both
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Móricz, Ágnes M., and Péter G. Ott. "Direct Bioautography as a High-Throughput Screening Method for the Detection of Antibacterial Components from Plant Sources." Journal of AOAC INTERNATIONAL 98, no. 4 (2015): 850–56. http://dx.doi.org/10.5740/jaoacint.sge1-moricz.

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Abstract The applicability of direct bioautography, the combination of planar chromatography with antimicrobial assay, is demonstrated with special emphasis on its recent developments such as BioArena and the use of genetically modified luminescent bacteria. Its methodological advancement is put into a historical perspective. In comparison with other commonly used antimicrobial susceptibility tests, the main advantage of direct bioautography resides in its simplicity, rapidity, and ability to detect separated individual matrix components exhibiting antimicrobial activity in situ. It is confirm
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Zang, Yichao, Zhihong Cheng, and Tao Wu. "TLC Bioautography on Screening of Bioactive Natural Products: An Update Review." Current Analytical Chemistry 16, no. 5 (2020): 545–56. http://dx.doi.org/10.2174/1573411015666181224145346.

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Background: TLC bioautography is a hyphenated technique combining planar chromatographic separation and in situ biological activity detection. This coupled method has been receiving much attention in screening bio-active natural products because of its properties of being simple, rapid, inexpensive, and effective. Methods: The recent progress in the development of method of TLC bioautography for detecting antimicrobial and enzyme inhibitory activities dating between 2012 and early 2018 has been reviewed. The applications of this method in biological screening of natural products were also pres
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Horváth, Györgyi, Noémi Jámbor, Erika Kocsis, et al. "Role of Direct Bioautographic Method for Detection of Antistaphylococcal Activity of Essential Oils." Natural Product Communications 6, no. 9 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600939.

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The aim of the present study was the chemical characterization of some traditionally used and therapeutically relevant essential oils (thyme, eucalyptus, cinnamon bark, clove, and tea tree) and the optimized microbiological investigation of the effect of these oils on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). The chemical composition of the oils was analyzed by TLC, and controlled by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The antibacterial effect was investigated using a TLC-bioautogr
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Grzelak, Edyta M., Wioleta Jesionek, Barbara Majer-Dziedzic, and Irena M. Choma. "Applications of Novel Direct Bioautography Tests for Analysis of Antimicrobials: A Review." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1167–74. http://dx.doi.org/10.5740/jaoacint.sgegrzelak.

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Abstract TLC coupled to direct bioautography detection can be applied to the analysis of various antimicrobial agents in complex matrixes. Because of the lack of commercially available microbiological detection methods, two direct bioautography tests were developed in our laboratory to be used after TLC separation. One method was based on Gram-positive bacteria, Bacillus subtilis, and the other on Gram-negative bacteria, Escherichia coli. These tests can be used for detection and determination of wide spectrum of antimicrobials as well as for other, nontypical purposes, such as choosing the be
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Horváth, Györgyi, Kamilla Ács, and Béla Kocsis. "TLC-Direct Bioautography for Determination of Antibacterial Activity of Artemisia adamsii Essential Oil." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1209–13. http://dx.doi.org/10.5740/jaoacint.sgehorvath.

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Abstract The aim of the present study was the chemical characterization of the essential oil of a Mongolian medicinal plant, Artemisia adamsii Besser, and the investigation of the antibacterial effect of its oil on different human pathogenic bacteria (Staphylococcus aureus, methicillin-resistant S. aureus, and S. epidermidis). The chemical composition of the oil was established by GC and GC/MS. Direct bioautography was used for detecting the antibacterial activity of the essential oil. The result of GC experiments showed that α-thujone was the main component (64.4%) of the oil, while the amoun
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Patil, Anita Surendra, Hariprasad Madhukarrao Paikrao, Ankit Subhash Kale, and Surendra Raghoba Manik. "A TLC-Direct Bioautography Method for Detection of Antiurolithiatic Metabolites." Journal of Chromatographic Science 55, no. 5 (2017): 578–85. http://dx.doi.org/10.1093/chromsci/bmx002.

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Choma, I. M. "Thin‐Layer Chromatography‐Direct Bioautography of Flumequine Residues in Milk." Journal of Liquid Chromatography & Related Technologies 29, no. 14 (2006): 2083–93. http://dx.doi.org/10.1080/10826070600759942.

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Choma, Irena, Adam Choma, and Krystyna Staszczuk. "Direct bioautography-thin-layer chromatography of flumequine and doxycycline in milk." Journal of Planar Chromatography – Modern TLC 15, no. 3 (2002): 187–91. http://dx.doi.org/10.1556/jpc.15.2002.3.5.

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Book chapters on the topic "Direct bioautography"

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Sherma, Joseph. "Studies on the Antioxidant Activity of Foods and Food Ingredients by Thin-Layer Chromatography–Direct Bioautography with 2,2′-Diphenyl-1-Picrylhydrazyl Radical (DPPH*)." In Advances in Chromatography. CRC Press, 2017. http://dx.doi.org/10.1201/9781315158075-9.

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