Relevant bibliographies by topics / Direct fluorescent antibody (DFA)

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Direct fluorescent antibody (DFA)'

Author: Grafiati
Published: 7 June 2025
Last updated: 2 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Direct fluorescent antibody (DFA).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Direct fluorescent antibody (DFA)"

1

Loeffelholz, Mike J., Curt J. Thompson, Karla S. Long, and Mary J. R. Gilchrist. "Comparison of PCR, Culture, and Direct Fluorescent-Antibody Testing for Detection of Bordetella pertussis." Journal of Clinical Microbiology 37, no. 9 (1999): 2872–76. http://dx.doi.org/10.1128/jcm.37.9.2872-2876.1999.

Full text
Abstract:
We prospectively compared the performance of culture, direct fluorescent-antibody testing (DFA), and an in-house-developed PCR test targeting the repeated insertion sequence IS481 for the detection of Bordetella pertussis in nasopharyngeal swab specimens. We tested 319 consecutive paired specimens on which all three tests were performed. A total of 59 specimens were positive by one or more tests. Of these, 5 were positive by all three tests, 2 were positive by culture and PCR, 16 were positive by PCR and DFA, 28 were positive by PCR only, and 8 were positive by DFA only. Any specimen positive
APA, Harvard, Vancouver, ISO, and other styles
2

Natalia, Lily, and Rahmat Setya Adji. "Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay." Jurnal Ilmu Ternak dan Veteriner 13, no. 2 (2014): 140–49. https://doi.org/10.14334/jitv.v13i2.607.

Full text
Abstract:
During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsula
APA, Harvard, Vancouver, ISO, and other styles
3

Dupuis, Michelle, Scott Brunt, Kim Appler, April Davis, and Robert Rudd. "Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States." Journal of Clinical Microbiology 53, no. 9 (2015): 2983–89. http://dx.doi.org/10.1128/jcm.01227-15.

Full text
Abstract:
Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test, was the focus of this project. Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensitivity, specificity, and ability to detect variants. Additio
APA, Harvard, Vancouver, ISO, and other styles
4

Chan, Edward L., Ken Brandt, and Greg B. Horsman. "Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus." Clinical Diagnostic Laboratory Immunology 8, no. 5 (2001): 909–12. http://dx.doi.org/10.1128/cdli.8.5.909-912.2001.

Full text
Abstract:
ABSTRACT A new rapid direct immunofluorescence assay, the SimulFluor direct fluorescent-antibody (DFA) assay, which can simultaneously detect herpes simplex virus types 1 and 2 (HSV-1 and -2) and varicella-zoster virus (VZV), was evaluated in comparison with our current standard procedures of (i) shell vial direct immunoperoxidase (shell vial IP) staining and cell culture for detection of HSV and (ii) cytospin DFA staining for VZV detection. A total of 517 vesicular, oral, genital, and skin lesion specimens were tested by all three procedures. For HSV detection, the SimulFluor DFA assay had an
APA, Harvard, Vancouver, ISO, and other styles
5

Landry, Marie L., Sandra Cohen, and David Ferguson. "Impact of Sample Type on Rapid Detection of Influenza Virus A by Cytospin-Enhanced Immunofluorescence and Membrane Enzyme-Linked Immunosorbent Assay." Journal of Clinical Microbiology 38, no. 1 (2000): 429–30. http://dx.doi.org/10.1128/jcm.38.1.429-430.2000.

Full text
Abstract:
ABSTRACT Cytospin-enhanced direct fluorescent-antibody assay (DFA) detected 49 (92.5%) and rapid membrane enzyme-linked immunosorbent assay (ELISA) detected 40 (75.5%) of 53 influenza virus A-positive samples. All 15 positive nasopharyngeal aspirates from children were detected by both tests. In contrast, 34 of 38 (89.5%) positive swabs from adults were detected by DFA, but only 25 (66%) were detected by ELISA.
APA, Harvard, Vancouver, ISO, and other styles
6

Dar, Arshud M., Sanjay Kapil, and Sagar M. Goyal. "Comparison of Immunohistochemistry, Electron Microscopy, and Direct Fluorescent Antibody Test for the Detection of Bovine Coronavirus." Journal of Veterinary Diagnostic Investigation 10, no. 2 (1998): 152–57. http://dx.doi.org/10.1177/104063879801000206.

Full text
Abstract:
Bovine coronavirus (BCV) is 1 of the major causes of calf diarrhea and has also been implicated in respiratory infections of young calves and winter dysentery of adult cattle. Currently, transmission electron microscopy (TEM), direct fluorescent antibody (DFA), and enzyme-linked immunosorbent assay (ELISA) techniques are considered standard methods for the diagnosis of BCV infection. However, these techniques are not useful if fresh tissues and intestinal contents are not available for examination. The detection of viral antigens in formalin-fixed, paraffin-embedded tissues using immunohistoch
APA, Harvard, Vancouver, ISO, and other styles
7

Orser, Lauren, and Patrick O’Byrne. "Direct fluorescence antibody testing augments syphilis diagnosis, compared to serology alone." International Journal of STD & AIDS 33, no. 2 (2021): 123–28. http://dx.doi.org/10.1177/09564624211048610.

Full text
Abstract:
In Ottawa, Canada, we initiated protocols to include non-serologic syphilis testing, as direct fluorescence antibody (DFA) for patients with syphilis symptoms. The purpose was to assess the ability of DFA to detect syphilis during acute infection and to determine if non-serologic testing could yield an increased number of syphilis diagnoses. We reviewed charts of patients of our local sexual health clinic for whom syphilis was suspected. A total of 69 clinical encounters were recorded for 67 unique patients, most of whom were male. The most common symptom was a painless genital lesion. Of the
APA, Harvard, Vancouver, ISO, and other styles
8

Reedy, Mark B., Patricia J. Sulak, William B. McCombs III, and Thomas J. Kuehl. "Performance of the Syva Direct Fluorescent Antibody Assay for Chlamydia in a Low-Prevalence Population." Infectious Diseases in Obstetrics and Gynecology 1, no. 1 (1993): 2–6. http://dx.doi.org/10.1155/s106474499300002x.

Full text
Abstract:
Chlamydia trachomatisis the most common reportable sexually transmitted disease (STD) in the United States. In the 1980s, rapid diagnostic tests for chlamydia began to replace more cumbersome tissue culture methods. Current data on rapid antigen detection assays demonstrate acceptable sensitivity, specificity, and predictive values in populations with a high prevalence of chlamydia. Few studies report the performance of these assays in a low-prevalence obstetric and gynecologic (Ob/Gyn) population, This study compares the most commonly used direct fluorescent antibody (DFA) assay (Syva Microtr
APA, Harvard, Vancouver, ISO, and other styles
9

Patwardhan, Vrushali, Preena Bhalla, Deepti Rawat, Vijay Kumar Garg, Kabir Sardana, and Sumit Sethi. "A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes." Journal of Laboratory Physicians 9, no. 01 (2017): 053–56. http://dx.doi.org/10.4103/0974-2727.187929.

Full text
Abstract:
ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was
APA, Harvard, Vancouver, ISO, and other styles
10

Purwoko, M. Izazi Hari, Mutia Devi, Suroso Adi Nugroho, Fitriani Fitriani, Raden Pamudji, and Nofilia Citra Candra. "Laboratory Examination of Syphilis." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 3 (2021): 722–41. http://dx.doi.org/10.32539/bsm.v5i3.339.

Full text
Abstract:
Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal dise
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Direct fluorescent antibody (DFA)"

1

Lemos, Ana Cláudia Cavalcante Espósito. "Estudo histomorfométrico, ultraestrutural e da expressão de Wnt1, WIF-1 e ASIP na pele com melasma em comparação com a pele sã perilesional e retroauricular." Botucatu, 2017. http://hdl.handle.net/11449/151232.

Full text
Abstract:
Orientador: Hélio Amante Miot<br>Resumo: O melasma é hipermelanose crônica e adquirida decorrente de um complexo processo que envolve hipertrofia melanocítica e disfunção melanogênica. Acomete preferencialmente o sexo feminino e as lesões ocorrem nas áreas fotoexpostas, especialmente a face. Sua patogênese não é bem compreendida e os estudos clássicos avaliam apenas pele acometida e perilesional, mas pouco se sabe do comportamento da pele fotoprotegida, submetida aos mesmos fatores sistêmicos e genéticos. Neste estudo, objetivamos avaliar características histológicas, vias epidérmicas que infl
APA, Harvard, Vancouver, ISO, and other styles
2

Lemos, Ana Cláudia Cavalcante Espósito [UNESP]. "Estudo histomorfométrico, ultraestrutural e da expressão de Wnt1, WIF-1 e ASIP na pele com melasma em comparação com a pele sã perilesional e retroauricular." Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/151232.

Full text
Abstract:
Submitted by Ana Cláudia Cavalcante Espósito null (anaclaudiaesposito@gmail.com) on 2017-07-27T20:34:29Z No. of bitstreams: 1 Ana Cláudia mestrado para depositar.pdf: 2933638 bytes, checksum: 3c192abd021482675fa186f4c4bba9e4 (MD5)<br>Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-07-31T19:29:06Z (GMT) No. of bitstreams: 1 lemos_acce_me_bot.pdf: 2933638 bytes, checksum: 3c192abd021482675fa186f4c4bba9e4 (MD5)<br>Made available in DSpace on 2017-07-31T19:29:06Z (GMT). No. of bitstreams: 1 lemos_acce_me_bot.pdf: 2933638 bytes, checksum:
APA, Harvard, Vancouver, ISO, and other styles
3

Miyamoto, Denise. "Avaliação da expressão do fator de crescimento endotelial vascular e da endoglina nos pacientes eritrodérmicos com pênfigo foliáceo." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-25082017-091409/.

Full text
Abstract:
INTRODUÇÃO: O pênfigo foliáceo (PF) caracteriza-se pela síntese de autoanticorpos contra a desmogleína 1 (Dsg1) com acantólise na epiderme superior. As lesões na face e tronco podem evoluir para eritrodermia (PFE), cuja patogênese é pouco conhecida. Estudos prévios sugerem a participação do fator de crescimento endotelial vascular (VEGF) e da endoglina (Eng). OBJETIVOS: Avaliar dados demográficos, expressão tecidual e níveis séricos do VEGF e Eng, bem como o perfil dos imunocomplexos no PFE. PACIENTES, MATERIAIS E MÉTODOS: Foram selecionados pacientes de PFE (n=31) e indivíduos controles com p
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Direct fluorescent antibody (DFA)"

1

Bhogilal Nayak, Jitendrakumar, Jeetendrakumar Harnathbhai Chaudhary, Prakrutik Prafulchandra Bhavsar, Pranav Ashok Anjaria, Manojbhai N. Brahmbhatt, and Urvish Pravinbhai Mistry. "Rabies: Incurable Biological Threat." In Zoonoses of Public Health Interest [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105079.

Full text
Abstract:
Rabies is a lethal zoonotic disease that affects all the homeotherms, including humans, and is caused by the Rabies virus of Rhabdoviridae family. Every year, this disease kills about 55,000 individuals globally. The stray dog is a key player in the spread of rabies. The disease is usually transmitted through the bite of a rabid animal. After being exposed to the virus, the virus must travel to the brain before generating symptoms. Delirium, unusual behaviour, hallucinations, hydrophobia and insomnia may occur as the condition advances. Diagnostic tests such as direct fluorescent antibody test
APA, Harvard, Vancouver, ISO, and other styles
2

Mayes, Bonny, and Charles E. Rupprecht. "Direct Fluorescent Antibody Test for Rabies Diagnosis." In Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 2. Elsevier, 2015. http://dx.doi.org/10.1016/b978-0-12-801919-1.00008-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Sheila, Moriber Katz. "Chapter 3: Diagnosis: Direct Fluorescent Antibody Test." In Legionellosis. CRC Press, 2019. http://dx.doi.org/10.1201/9781351073981-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Embleton, M. Jim. "Reverse transcriptase in situ PCR for RNA detection." In PCR3. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636327.003.0005.

Full text
Abstract:
Abstract Most published applications of in situ PCR for amplification of specific nucleic acid sequences in cells or tissues have been for the detection of genes or viruses which are present in low copy numbers or in a minority of the cell population. Detection may be either indirect, for example by hybridizing a labelled oligonucleotide probe to the amplified DNA, or direct, where the product is amplified with labelled oligonucleotide primers. A variety of labels for oligonucleotides have been used in different situations, including fluorescent dyes for direct microscopic visualization (1,2),
APA, Harvard, Vancouver, ISO, and other styles
5

"Fluorescent antibody techniques have allowed for the direct identification and enumeration of individual bacteria in environmental samples without requiring prior growth in culture media (Bahlool and Schmidt 1980, Cloete and Steyn 1988, Macario et al. 1989). The technique involves the use of specific antibodies raised against surface markers of defined pure cultures that are either labelled directly with fluorescent dye molecules or via a fluorescent secondary antibody. This approach has yielded important insights into the spatial distribution of microorganisms, but it suffers from a number of disadvantages. For example, expression of the antigen may be influenced by environmental factors; false-positive and false-negative results may be obtained due to cross-reactivity or lack of reaction; non-specific binding of antibodies may result in high levels of background fluorescence; and production of specific antibodies requires a pure culture of the organism of interest (Cloete and de Bruyn Various recombinant DNA techniques have subsequently been developed that are independent of cultivation methods (Fig. 1). These techniques provide ways of detecting and quantifying specific phylogenetic groups of microbes on 16S rDNA sequences, and relevant structural genes provide ways of monitoring microbial populations of environmental and industrial systems. In addition to these tools, a number of emerging technologies such as the use of biomarker genes are being increasingly used to monitor with great precision and accuracy the behaviour of microbes in the environment." In Recent Advances in Marine Biotechnology, Vol. 8. CRC Press, 2003. http://dx.doi.org/10.1201/9781482279986-12.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Direct fluorescent antibody (DFA)"

1

Rhee, Chanu, Janet H. Kim, Joanna Schaenman, et al. "Difficulty In Rapid Diagnosis Of Novel Influenza A (H1N1) Virus Using Direct Fluorescent Antibody Testing (DFA) In Critically Versus Non-critically Ill Patients." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a2624.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Direct fluorescent antibody (DFA)' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography