Log in

Relevant bibliographies by topics / Direct fluorescent antibody (DFA) / Journal articles

To see the other types of publications on this topic, follow the link: Direct fluorescent antibody (DFA).

Journal articles on the topic 'Direct fluorescent antibody (DFA)'

Author: Grafiati

Published: 7 June 2025

Last updated: 17 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Direct fluorescent antibody (DFA).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Loeffelholz, Mike J., Curt J. Thompson, Karla S. Long, and Mary J. R. Gilchrist. "Comparison of PCR, Culture, and Direct Fluorescent-Antibody Testing for Detection of Bordetella pertussis." Journal of Clinical Microbiology 37, no. 9 (1999): 2872–76. http://dx.doi.org/10.1128/jcm.37.9.2872-2876.1999.

Full text

Abstract:

We prospectively compared the performance of culture, direct fluorescent-antibody testing (DFA), and an in-house-developed PCR test targeting the repeated insertion sequence IS481 for the detection of Bordetella pertussis in nasopharyngeal swab specimens. We tested 319 consecutive paired specimens on which all three tests were performed. A total of 59 specimens were positive by one or more tests. Of these, 5 were positive by all three tests, 2 were positive by culture and PCR, 16 were positive by PCR and DFA, 28 were positive by PCR only, and 8 were positive by DFA only. Any specimen positive

APA, Harvard, Vancouver, ISO, and other styles

2

Natalia, Lily, and Rahmat Setya Adji. "Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay." Jurnal Ilmu Ternak dan Veteriner 13, no. 2 (2014): 140–49. https://doi.org/10.14334/jitv.v13i2.607.

Full text

Abstract:

During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsula

APA, Harvard, Vancouver, ISO, and other styles

3

Dupuis, Michelle, Scott Brunt, Kim Appler, April Davis, and Robert Rudd. "Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States." Journal of Clinical Microbiology 53, no. 9 (2015): 2983–89. http://dx.doi.org/10.1128/jcm.01227-15.

Full text

Abstract:

Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test, was the focus of this project. Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensitivity, specificity, and ability to detect variants. Additio

APA, Harvard, Vancouver, ISO, and other styles

4

Chan, Edward L., Ken Brandt, and Greg B. Horsman. "Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus." Clinical Diagnostic Laboratory Immunology 8, no. 5 (2001): 909–12. http://dx.doi.org/10.1128/cdli.8.5.909-912.2001.

Full text

Abstract:

ABSTRACT A new rapid direct immunofluorescence assay, the SimulFluor direct fluorescent-antibody (DFA) assay, which can simultaneously detect herpes simplex virus types 1 and 2 (HSV-1 and -2) and varicella-zoster virus (VZV), was evaluated in comparison with our current standard procedures of (i) shell vial direct immunoperoxidase (shell vial IP) staining and cell culture for detection of HSV and (ii) cytospin DFA staining for VZV detection. A total of 517 vesicular, oral, genital, and skin lesion specimens were tested by all three procedures. For HSV detection, the SimulFluor DFA assay had an

APA, Harvard, Vancouver, ISO, and other styles

5

Landry, Marie L., Sandra Cohen, and David Ferguson. "Impact of Sample Type on Rapid Detection of Influenza Virus A by Cytospin-Enhanced Immunofluorescence and Membrane Enzyme-Linked Immunosorbent Assay." Journal of Clinical Microbiology 38, no. 1 (2000): 429–30. http://dx.doi.org/10.1128/jcm.38.1.429-430.2000.

Full text

Abstract:

ABSTRACT Cytospin-enhanced direct fluorescent-antibody assay (DFA) detected 49 (92.5%) and rapid membrane enzyme-linked immunosorbent assay (ELISA) detected 40 (75.5%) of 53 influenza virus A-positive samples. All 15 positive nasopharyngeal aspirates from children were detected by both tests. In contrast, 34 of 38 (89.5%) positive swabs from adults were detected by DFA, but only 25 (66%) were detected by ELISA.

APA, Harvard, Vancouver, ISO, and other styles

6

Dar, Arshud M., Sanjay Kapil, and Sagar M. Goyal. "Comparison of Immunohistochemistry, Electron Microscopy, and Direct Fluorescent Antibody Test for the Detection of Bovine Coronavirus." Journal of Veterinary Diagnostic Investigation 10, no. 2 (1998): 152–57. http://dx.doi.org/10.1177/104063879801000206.

Full text

Abstract:

Bovine coronavirus (BCV) is 1 of the major causes of calf diarrhea and has also been implicated in respiratory infections of young calves and winter dysentery of adult cattle. Currently, transmission electron microscopy (TEM), direct fluorescent antibody (DFA), and enzyme-linked immunosorbent assay (ELISA) techniques are considered standard methods for the diagnosis of BCV infection. However, these techniques are not useful if fresh tissues and intestinal contents are not available for examination. The detection of viral antigens in formalin-fixed, paraffin-embedded tissues using immunohistoch

APA, Harvard, Vancouver, ISO, and other styles

7

Orser, Lauren, and Patrick O’Byrne. "Direct fluorescence antibody testing augments syphilis diagnosis, compared to serology alone." International Journal of STD & AIDS 33, no. 2 (2021): 123–28. http://dx.doi.org/10.1177/09564624211048610.

Full text

Abstract:

In Ottawa, Canada, we initiated protocols to include non-serologic syphilis testing, as direct fluorescence antibody (DFA) for patients with syphilis symptoms. The purpose was to assess the ability of DFA to detect syphilis during acute infection and to determine if non-serologic testing could yield an increased number of syphilis diagnoses. We reviewed charts of patients of our local sexual health clinic for whom syphilis was suspected. A total of 69 clinical encounters were recorded for 67 unique patients, most of whom were male. The most common symptom was a painless genital lesion. Of the

APA, Harvard, Vancouver, ISO, and other styles

8

Reedy, Mark B., Patricia J. Sulak, William B. McCombs III, and Thomas J. Kuehl. "Performance of the Syva Direct Fluorescent Antibody Assay for Chlamydia in a Low-Prevalence Population." Infectious Diseases in Obstetrics and Gynecology 1, no. 1 (1993): 2–6. http://dx.doi.org/10.1155/s106474499300002x.

Full text

Abstract:

Chlamydia trachomatisis the most common reportable sexually transmitted disease (STD) in the United States. In the 1980s, rapid diagnostic tests for chlamydia began to replace more cumbersome tissue culture methods. Current data on rapid antigen detection assays demonstrate acceptable sensitivity, specificity, and predictive values in populations with a high prevalence of chlamydia. Few studies report the performance of these assays in a low-prevalence obstetric and gynecologic (Ob/Gyn) population, This study compares the most commonly used direct fluorescent antibody (DFA) assay (Syva Microtr

APA, Harvard, Vancouver, ISO, and other styles

9

Patwardhan, Vrushali, Preena Bhalla, Deepti Rawat, Vijay Kumar Garg, Kabir Sardana, and Sumit Sethi. "A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes." Journal of Laboratory Physicians 9, no. 01 (2017): 053–56. http://dx.doi.org/10.4103/0974-2727.187929.

Full text

Abstract:

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was

APA, Harvard, Vancouver, ISO, and other styles

10

Purwoko, M. Izazi Hari, Mutia Devi, Suroso Adi Nugroho, Fitriani Fitriani, Raden Pamudji, and Nofilia Citra Candra. "Laboratory Examination of Syphilis." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 3 (2021): 722–41. http://dx.doi.org/10.32539/bsm.v5i3.339.

Full text

Abstract:

Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal dise

APA, Harvard, Vancouver, ISO, and other styles

11

Purwoko, M. Izazi Hari, Mutia Devi, Suroso Adi Nugroho, Fitriani Fitriani, Raden Pamudji, and Nofilia Citra Candra. "Laboratory Examination of Syphilis." Bioscientia Medicina : Journal of Biomedicine and Translational Research 5, no. 8 (2021): 726–45. http://dx.doi.org/10.32539/bsm.v5i8.339.

Full text

Abstract:

Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal dise

APA, Harvard, Vancouver, ISO, and other styles

12

Polupan, I. M. "Direct fluorescent antibody test in laboratory diagnosis of animal rabies in Ukraine." Veterinary Medicine: inter-departmental subject scientific collection, no. 107 (November 8, 2021): 15–18. http://dx.doi.org/10.36016/vm-2021-107-2.

Full text

Abstract:

The aim of the study was to analyze the role of the direct fluorescent antibody (DFA) test in the system of laboratory diagnosis of animal rabies in Ukraine. For the analysis, materials of official veterinary reporting were used according to Form No. 2-VET “Report on the work of the state laboratories of veterinary medicine” regarding the results of laboratory studies of pathological material suspicious of rabies, the State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise (SRILDVSE) and virology departments of the State Regional Laboratories of the State Food

APA, Harvard, Vancouver, ISO, and other styles

13

Prabhu, K. Nithin, Shrikrishna Isloor, B. Hanchinal Veeresh, et al. "Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India." Veterinary Sciences 5, no. 1 (2018): 24. https://doi.org/10.5281/zenodo.13530561.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as the more sensitive, reverse transcription polymerase chain reaction (RT-PCR), have recently been employed for the laboratory diagnosis of rabies. We compared the three methods on brain samples from domestic (dog, cat, cattle, buffalo, horse, pig and goat) and wild (leopard, wolf and jackal) anim

APA, Harvard, Vancouver, ISO, and other styles

14

Prabhu, K. Nithin, Shrikrishna Isloor, B. Hanchinal Veeresh, et al. "Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India." Veterinary Sciences 5, no. 1 (2018): 24. https://doi.org/10.5281/zenodo.13530561.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as the more sensitive, reverse transcription polymerase chain reaction (RT-PCR), have recently been employed for the laboratory diagnosis of rabies. We compared the three methods on brain samples from domestic (dog, cat, cattle, buffalo, horse, pig and goat) and wild (leopard, wolf and jackal) anim

APA, Harvard, Vancouver, ISO, and other styles

15

Bao, Jian R., Ronald N. Master, Robert S. Jones, Richard B. Clark, and Kileen L. Shier. "723. Cryptosporidium Detection in Preserved Stool Specimens: A Comparison Study of EIA, DFA, and Direct Microscopic Method." Open Forum Infectious Diseases 8, Supplement_1 (2021): S460. http://dx.doi.org/10.1093/ofid/ofab466.920.

Full text

Abstract:

Abstract Background Cryptosporidium is an intestinal parasite that may cause diarrhea. Laboratory diagnosis largely relies on microscopic or immunology-based antigen detection. Direct fluorescent antibody (DFA) is considered the gold standard. Enzyme immunoassay (EIA) is an attractive alternative, but direct comparison studies for the performance together with the impact from different specimen preservation media are limiting. Methods We compared these three methods for the detection of Cryptosporidium oocysts (direct microscopic) or antigen (DFA or EIA) from stool samples preserved in either

APA, Harvard, Vancouver, ISO, and other styles

16

Thomas, B. J., T. Pierpoint, D. Taylor Robinson, and A. M. Renton. "Quantification of Chlamydia trachomatis in cervical and urine specimens from women attending a genitourinary medicine clinic: implications for screening strategies." International Journal of STD & AIDS 9, no. 8 (1998): 448–51. http://dx.doi.org/10.1258/0956462981922601.

Full text

Abstract:

Population screening and intervention programmes can reduce the prevalence and incidence of infection with Chlamydia trachomatis , especially if sensitive molecular diagnostic tests are used. However, diagnostic tests that perform well on genitourinary medicine GUM clinic populations may be less useful for screening, particularly if the majority of infected subjects are asymptomatic and their samples contain fewer organisms. We have compared the extent of low organism load in cervical and urine samples from symptomatic and asymptomatic chlamydia positive women, by using a direct fluorescent an

APA, Harvard, Vancouver, ISO, and other styles

17

Dettinger, Lisa, Crystal M. Gigante, Maria Sellard, et al. "Detection of Apparent Early Rabies Infection by LN34 Pan-Lyssavirus Real-Time RT-PCR Assay in Pennsylvania." Viruses 14, no. 9 (2022): 1845. http://dx.doi.org/10.3390/v14091845.

Full text

Abstract:

The Pennsylvania Department of Health Bureau of Laboratories (PABOL) tested 6855 animal samples for rabies using both the direct fluorescent antibody test (DFA) and LN34 pan-lyssavirus reverse transcriptase quantitative PCR (RT-qPCR) during 2017–2019. Only two samples (0.03%) were initially DFA negative but positive by LN34 RT-qPCR. Both cases were confirmed positive upon re-testing at PABOL and confirmatory testing at the Centers for Disease Control and Prevention by LN34 RT-qPCR and DFA. Rabies virus sequences from one sample were distinct from all positive samples processed at PABOL within

APA, Harvard, Vancouver, ISO, and other styles

18

Perin, Patricia Parreira, Talita Turmina, Carmen Andrea Arias-Pacheco, et al. "Rabies Virus-Neutralizing Antibodies in Free-Ranging Invasive Wild Boars (Sus scrofa) from Brazil." Pathogens 13, no. 4 (2024): 303. http://dx.doi.org/10.3390/pathogens13040303.

Full text

Abstract:

Rabies, one of the most lethal global zoonoses, affects all mammals. It remains circulating worldwide in sylvatic cycles through terrestrial and airborne reservoirs, and in Brazil, bats are currently the main reservoirs and source of transmission. Wild boars, an important invasive alien species in Brazil, are a proven food source for hematophagous bats and may participate in the Brazilian sylvatic cycle of rabies. We evaluated the presence of this pathogen in hunted wild boars from the São Paulo state using histopathology, the direct fluorescent antibody test (DFA), viral isolation in cell cul

APA, Harvard, Vancouver, ISO, and other styles

19

Caliendo, Angela M., Peter L. Hewitt, Jessica M. Allega, Anne Keen, Kathryn L. Ruoff, and Mary Jane Ferraro. "Performance of a PCR Assay for Detection of Pneumocystis carinii from Respiratory Specimens." Journal of Clinical Microbiology 36, no. 4 (1998): 979–82. http://dx.doi.org/10.1128/jcm.36.4.979-982.1998.

Full text

Abstract:

This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique for detection of amplicons, and an internal control to evaluate for the presence of inhibitors of amplification. Two hundred thirty-two clinical specimens (120 induced-sputum [IS] and 112 bronchoalveolar lavage [BAL] specimens) from 168 patients were tested by both immunofluoresce

APA, Harvard, Vancouver, ISO, and other styles

20

Dean, Deborah, Dennis Ferrero, and Michael McCarthy. "Comparison of Performance and Cost-Effectiveness of Direct Fluorescent-Antibody, Ligase Chain Reaction, and PCR Assays for Verification of Chlamydial Enzyme Immunoassay Results for Populations with a Low to Moderate Prevalence of Chlamydia trachomatis Infection." Journal of Clinical Microbiology 36, no. 1 (1998): 94–99. http://dx.doi.org/10.1128/jcm.36.1.94-99.1998.

Full text

Abstract:

Many laboratories use a commercial enzyme immunoassay (EIA) with verification testing to diagnose Chlamydia trachomatisinfections in an effort to contain costs. This study was designed to compare the performance and cost-effectiveness of direct fluorescent-antibody assay (DFA), commercial PCR, and ligase chain reaction (LCR) for the verification of EIA results. Cervical specimens were screened by EIA. DFA, PCR, and LCR were compared as verification tests for EIA-reactive specimens and negative greyzone (NGZ) specimens at 50% below the cutoff value. These samples were also tested by in-house PC

APA, Harvard, Vancouver, ISO, and other styles

21

Miller, Darlene, Jorge Mesa-Maestre, Edith Perez, Benjamin David Wilson, and Eduardo C. Alfonso. "Prevalence of Ocular Chlamydial Infections in South Florida." US Ophthalmic Review 09, no. 02 (2016): 102. http://dx.doi.org/10.17925/usor.2016.09.02.102.

Full text

Abstract:

Ocular chlamydia in the US is a sexually transmitted disease. The true public health burden is unknown. We used a combination of culture and culture independent tests to document disease prevalence over a 28-year (1986–2014) period.Methods:Laboratory records of patient’s samples (n=3,112) submitted to rule out chlamydia were reviewed. Data were extracted for patient’s demographics (age, sex), age and positivity rates for historical methods (1986–2004), direct fluorescent antibody (DFA; n=1,774), culture (n=1,619), nucleic acid amplification tests (NAAT; n=274) and enzyme immunoassay tests (EIA

APA, Harvard, Vancouver, ISO, and other styles

22

TORTORELLO, M. L., K. F. REINEKE, D. S. STEWART, and R. B. RAYBOURNE. "Comparison of Methods for Determining the Presence of Escherichia coli O157:H7 in Apple Juice." Journal of Food Protection 61, no. 11 (1998): 1425–30. http://dx.doi.org/10.4315/0362-028x-61.11.1425.

Full text

Abstract:

Six methods were compared for detection of three strains of Escherichia coli O157:H7 in enrichments of inoculated apple juice. Juice was inoculated at levels varying from 0.1 to 100 CFU/ml and centrifuged after ovemight storage at 4°C, and pellets were incubated at 37°C in nonselective enrichment broth. At hourly intervals between 5 and 10 h and at 24 h, the enrichments were tested for E. coli O157:H7 by direct fluorescent antibody (DFA), antibody-direct epifluorescent filter technique (Ab-DEFT), direct selective plating on sorbitol MacConkey agar (SMA), immunomagnetic separation coupled to ei

APA, Harvard, Vancouver, ISO, and other styles

23

HALFORD, J. A., P. W. SHIELD, and R. G. WRIGHT. "The value of direct fluorescent antibody (DFA) testing for the detection of Pneumocystis Carinii In cytological specimens." Cytopathology 5, no. 4 (1994): 234–42. http://dx.doi.org/10.1111/j.1365-2303.1994.tb00425.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Hasan, Jafrul A. K., David Bernstein, Anwarul Huq, Lawrence Loomis, Mark L. Tamplin, and Rita R. Colwell. "Cholera DFA: An improved direct fluorescent monoclonal antibody staining kit for rapid detection and enumeration ofVibrio choleraeO1." FEMS Microbiology Letters 120, no. 1-2 (1994): 143–48. http://dx.doi.org/10.1111/j.1574-6968.1994.tb07021.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Krilov, Leonard R., Stephen R. Barone, S. Hella Harkness, Steven M. Lipson, Mark H. Kaplan, and Zarui Ciamician. "Evaluation of a Rapid Diagnostic Test for Respiratory Syncytial Virus (RSV): Potential for Bedside Diagnosis." Pediatrics 93, no. 6 (1994): 903–6. http://dx.doi.org/10.1542/peds.93.6.903.

Full text

Abstract:

Objective. Rapid detection of respiratory syncytial virus (RSV) infection can assist clinicians in decisions regarding antiviral therapy with ribavirin as well as instituting infection control measures. The Abbott TestPack RSV is a rapid RSV detection immunoassay that can be performed on respiratory secretions in 20 to 30 minutes without special laboratory equipment The purpose of this study was to evaluate housestaff performance of the TestPack RSV at bedside as compared with laboratory testing of aliquots of the same specimen by tissue culture inoculation, direct fluorescent antibody (DFA) t

APA, Harvard, Vancouver, ISO, and other styles

26

Newhall, Wilbert J., Robert E. Johnson, Susan DeLisle, et al. "Head-to-Head Evaluation of Five Chlamydia Tests Relative to a Quality-Assured Culture Standard." Journal of Clinical Microbiology 37, no. 3 (1999): 681–85. http://dx.doi.org/10.1128/jcm.37.3.681-685.1999.

Full text

Abstract:

Nucleic acid amplification tests offer superior sensitivity for the detection of Chlamydia trachomatis infection, but many laboratories still use nonamplification methods because of the lower cost and ease of use. In spite of their availability for more than a decade, few studies have directly compared the nonamplification tests. Such comparisons are still needed in addition to studies that directly compare individual nonamplification and amplification tests. The purpose of this study was to evaluate and compare the performance characteristics relative to culture of five different tests for th

APA, Harvard, Vancouver, ISO, and other styles

27

Ganzenmueller, Tina, Jeanette Kluba, Birgit Hilfrich, et al. "Comparison of the performance of direct fluorescent antibody staining, a point-of-care rapid antigen test and virus isolation with that of RT-PCR for the detection of novel 2009 influenza A (H1N1) virus in respiratory specimens." Journal of Medical Microbiology 59, no. 6 (2010): 713–17. http://dx.doi.org/10.1099/jmm.0.017244-0.

Full text

Abstract:

Although infections with the novel pandemic 2009 influenza A (H1N1) virus (A/H1N1/2009) appeared to be relatively mild during the first summer of circulation (‘off season’), there has been significant morbidity and hospitalization and several fatal cases. Thus, rapid detection of A/H1N1/2009 is crucial for efficient treatment and infection control measures. In contrast to seasonal influenza, where point-of-care (POC) rapid antigen tests and direct fluorescent antibody (DFA) staining ensure rapid detection, diagnosis of A/H1N1/2009 has so far been based on RT-PCR. This study retrospectively com

APA, Harvard, Vancouver, ISO, and other styles

28

Trottier, Caitlin A., Christina F. Yen, Grace Malvar, Jon Arnason, David E. Avigan, and Carolyn D. Alonso. "Case Report: Refractory Cryptosporidiosis after CAR T-Cell Therapy for Lymphoma." American Journal of Tropical Medicine and Hygiene 105, no. 3 (2021): 651–53. http://dx.doi.org/10.4269/ajtmh.21-0246.

Full text

Abstract:

ABSTRACT. Cryptosporidial diarrhea is uncommon in immunocompetent individuals, more often seen in severely immunocompromised patients. Severe refractory cases have been described in patients with HIV/AIDS before the advent of modern antiretroviral therapy due to an inability to mount an adequate cellular immune response. We describe an 85-year-old patient post–chimeric antigen receptor T-cell therapy relapsed lymphoma who developed refractory Cryptosporidium spp. diarrhea in the setting of persistent CD4+ cytopenia. Despite receiving multiple antiparasitic agents, including failure of a prolon

APA, Harvard, Vancouver, ISO, and other styles

29

Alam, Munirul, Abdus Sadique, Nur-A-Hasan, et al. "Effect of Transport at Ambient Temperature on Detection and Isolation of Vibrio cholerae from Environmental Samples." Applied and Environmental Microbiology 72, no. 3 (2006): 2185–90. http://dx.doi.org/10.1128/aem.72.3.2185-2190.2006.

Full text

Abstract:

ABSTRACT It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting environmental samples at the ambient air temperature on isolation and enumeration of V. cholerae. For water and plankton samples held at ambient temperatures ranging from 31°C to 35°C for 20 h, the total counts did not increase significantly but the number of culturable V. chol

APA, Harvard, Vancouver, ISO, and other styles

30

Rahi, Abdulsadah Abdulabbas. "Comparison among Modified Acid-Fast Stain and Some Immunological Methods in Diagnosis of Cryptosporidium parvum in Kut City." Journal of Wasit for Science and Medicine 6, no. 1 (2022): 1–9. http://dx.doi.org/10.31185/jwsm.187.

Full text

Abstract:

A total of 100 children who attending to Al-Karamah Teaching Hospital at Kut city were suffered from watery diarrhoea and abdominal pain were examined. The present study was conducted from October 2011 to January 2012. A thirty-four stool samples in which Cryptosporidium parvum oocysts had been seen by modified acid –fast stain examination were investigated, positivity detected with the ImmunoCard method, and Direct Fluorescent Antibody( DFA ) method was found to be (30%), (28%) and (34%) respectively.Children aged one month to 144 months presenting with acute or persistent diarrhoea were sele

APA, Harvard, Vancouver, ISO, and other styles

31

Alam, Munirul, Marzia Sultana, G. Balakrish Nair, et al. "Toxigenic Vibrio cholerae in the Aquatic Environment of Mathbaria, Bangladesh." Applied and Environmental Microbiology 72, no. 4 (2006): 2849–55. http://dx.doi.org/10.1128/aem.72.4.2849-2855.2006.

Full text

Abstract:

ABSTRACT Toxigenic Vibrio cholerae, rarely isolated from the aquatic environment between cholera epidemics, can be detected in what is now understood to be a dormant stage, i.e., viable but nonculturable when standard bacteriological methods are used. In the research reported here, biofilms have proved to be a source of culturable V. cholerae, even in nonepidemic periods. Biweekly environmental surveillance for V. cholerae was carried out in Mathbaria, an area of cholera endemicity adjacent to the Bay of Bengal, with the focus on V. cholerae O1 and O139 Bengal. A total of 297 samples of water,

APA, Harvard, Vancouver, ISO, and other styles

32

Pate, Mitchell S., Paula B. Dixon, Kim Hardy, Mark Crosby, and E. W. Hook. "Evaluation of the Biostar Chlamydia OIA Assay with Specimens from Women Attending a Sexually Transmitted Disease Clinic." Journal of Clinical Microbiology 36, no. 8 (1998): 2183–86. http://dx.doi.org/10.1128/jcm.36.8.2183-2186.1998.

Full text

Abstract:

Chlamydia trachomatis infections are the most prevalent sexually transmitted diseases (STDs) in the United States. In acute-care settings such as clinics and emergency rooms, a desirable chlamydia screening assay should exhibit good sensitivity and good specificity and should provide test results while the patient is still present. The Biostar Chlamydia OIA (Biostar, Inc., Boulder, Colo.) is an optical immunoassay (OIA) that provides test results in less than 30 min and that uses a test format that allows office-based testing. This assay is performed entirely at room temperature without the ne

APA, Harvard, Vancouver, ISO, and other styles

33

Atwill, Edward R., James A. Harp, Ted Jones, Phillip W. Jardon, Stephanie Checel, and Mike Zylstra. "Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection." American Journal of Veterinary Research 59, no. 9 (1998): 1116–21. http://dx.doi.org/10.2460/ajvr.1998.59.09.1116.

Full text

Abstract:

Abstract Objective To determine whether periparturient cows or contact surfaces to which newborn calves are exposed are reservoirs of Cryptosporidium parvum oocysts. Animals Periparturient cows and their calves. Procedure Using direct fluorescent antibody (DFA) and acid-fast (AF) assays, fecal samples taken before and after calving from periparturient cows were tested for C parvum oocysts. Fecal samples from calves were collected every other day from age 7 to 21 days and were tested by use of the AF assay. Topsoil from close-up and maternity pens and scrapings from wooden walls and floors of c

APA, Harvard, Vancouver, ISO, and other styles

34

Kumar, Pawan, Tamer A. Sharafeldin, Rahul Kumar, et al. "Development of a Recombinant Pichinde Virus-Vectored Vaccine against Turkey Arthritis Reovirus and Its Immunological Response Characterization in Vaccinated Animals." Pathogens 10, no. 2 (2021): 197. http://dx.doi.org/10.3390/pathogens10020197.

Full text

Abstract:

Vaccination may be an effective way to reduce turkey arthritis reovirus (TARV)-induced lameness in turkey flocks. However, there are currently no commercial vaccines available against TARV infection. Here, we describe the use of reverse genetics technology to generate a recombinant Pichinde virus (PICV) that expresses the Sigma C and/or Sigma B proteins of TARV as antigens. Nine recombinant PICV-based TARV vaccines were developed carrying the wild-type S1 (Sigma C) and/or S3 (Sigma B) genes from three different TARV strains. In addition, three recombinant PICV-based TARV vaccines were produced

APA, Harvard, Vancouver, ISO, and other styles

35

Theel, Elitza S., Samantha S. Katz, and Allan Pillay. "Molecular and Direct Detection Tests for Treponema pallidum Subspecies pallidum: A Review of the Literature, 1964–2017." Clinical Infectious Diseases 71, Supplement_1 (2020): S4—S12. http://dx.doi.org/10.1093/cid/ciaa176.

Full text

Abstract:

Abstract Direct detection methods for Treponema pallidum include dark-field microscopy (DFM), direct fluorescence antibody (DFA) testing, immunohistochemistry (IHC), and nucleic acid amplification tests (NAATs). Here, we reviewed the relevant syphilis diagnostic literature to address 2 main questions with respect to T. pallidum direct detection techniques: “What are the performance characteristics for each direct detection test for T. pallidum and what are the optimal specimen types for each test?” and “What options are available for T. pallidum molecular epidemiology?” To answer these questio

APA, Harvard, Vancouver, ISO, and other styles

36

Madison-Antenucci, S., R. F. Relich, L. Doyle, et al. "Multicenter Evaluation of BD Max Enteric Parasite Real-Time PCR Assay for Detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica." Journal of Clinical Microbiology 54, no. 11 (2016): 2681–88. http://dx.doi.org/10.1128/jcm.00765-16.

Full text

Abstract:

Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis , Entamoeba histolytica , Cryptosporidium parvum , and Cryptosporidium hominis . Similarly, these species cause the majority of parasitic diarrhea acquired in the United States. Detection of parasites by gold standard microscopic methods is time-consuming and requires considerable expertise; enzyme immunoassays and direct fluorescent-antibody (DFA) stains have lowered hands-on time for testing, but improvements in sensitivity and technic

APA, Harvard, Vancouver, ISO, and other styles

37

Tyagi, Anuj Kumar, Bijay Ranjan Mirdha, Kalpana Luthra, et al. "Dihydropteroate synthase (DHPS) gene mutation study in HIV-Infected Indian patients with Pneumocystis jirovecii pneumonia." Journal of Infection in Developing Countries 4, no. 11 (2010): 761–66. http://dx.doi.org/10.3855/jidc.914.

Full text

Abstract:

Introduction: Pneumocystis jirovecii dihydropteroate synthase (DHPS) gene mutations' (55th and 57th codon) association with prior sulfa prophylaxis failure has been reported from both developed and developing countries. We conducted a prospective study to determine the prevalence of P. jirovecii DHPS mutations from 2006 to 2009 on P. jirovecii isolates obtained from HIV-infected patients with a clinical diagnosis of Pneumocystis carinii pneumonia (PCP) admitted to our tertiary care reference health center in New Delhi, India. Methodology: Detection of P. jirovecii cysts was performed by direct

APA, Harvard, Vancouver, ISO, and other styles

38

Akter, T., S. Tabassum, A. Nessa, and M. Jahan. "A simple biological marker to differentiate the types of Herpes Simplex Viruses in resource-limited settings." Bangladesh Medical Research Council Bulletin 38, no. 1 (2012): 23–26. http://dx.doi.org/10.3329/bmrcb.v38i1.10448.

Full text

Abstract:

Herpes simplex viruses (HSV) multiply readily on the chorioallantoic membrane (CAM) of embryonated hens egg and produce easily visible foci or pocks on this membrane. In the present study, pocks produced by the two antigenic types of HSV (1 & 2) were compared to evaluate the effectiveness of typing HSV isolates by pock size on CAMs. A total of 57 HSV isolates from both non-genital and genital samples were typed by the pock size produced on the CAMs of fertile hens eggs. Twenty two HSV isolates yielded visible pocks on CAM, of which 9 (40.9%) produced small pocks, while 13 (59.1%) produce

APA, Harvard, Vancouver, ISO, and other styles

39

Eze, Ukamaka U., Ernest C. Ngoepe, Boniface M. Anene, Romanus C. Ezeokonkwo, Chika I. Nwosuh, and Claude T. Sabeta. "Molecular Detection of Rabies Lyssaviruses from Dogs in Southeastern Nigeria: Evidence of TransboundaryTransmission of Rabies in West Africa." Viruses 12, no. 2 (2020): 134. http://dx.doi.org/10.3390/v12020134.

Full text

Abstract:

Despite being the first country to register confirmed cases of Mokola and Lagos bat lyssaviruses (two very distant lyssaviruses), knowledge gaps, particularly on the molecular epidemiology of lyssaviruses, still exist in Nigeria. A total of 278 specimens were collected from dogs in southeastern Nigeria between October 2015 and July 2016, and 23 (8.3%) of these tested positive for lyssaviruses with the direct fluorescent antibody test (DFA). The lyssaviruses were genetically characterized by amplifying the highly conserved nucleoprotein (N) gene of the rabies lyssaviruses (RABVs) of the viral g

APA, Harvard, Vancouver, ISO, and other styles

40

Ferrero, Dennis V., Holly N. Meyers, Diane E. Schultz, and Stephen A. Willis. "Performance of the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay in Detecting Chlamydia trachomatis in Endocervical and Urine Specimens from Women and Urethral and Urine Specimens from Men Attending Sexually Transmitted Disease and Family Planning Clinics." Journal of Clinical Microbiology 36, no. 11 (1998): 3230–33. http://dx.doi.org/10.1128/jcm.36.11.3230-3233.1998.

Full text

Abstract:

The Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) uses transcription-mediated amplification and hybridization protection assay procedures to qualitatively detect Chlamydia trachomatisrRNA in urine, endocervical swab, and urethral specimens. The performance of the AMP CT was compared to that of cell culture for endocervical swab and urine specimens from women and urethral and urine specimens from men. Analysis of specimens with discrepant results was performed by a combination of reculture, direct fluorescent-antibody (DFA) staining of specimen sediment, and amplification which targe

APA, Harvard, Vancouver, ISO, and other styles

41

Adell, A. D., W. A. Smith, K. Shapiro, A. Melli, and P. A. Conrad. "Molecular Epidemiology of Cryptosporidium spp. and Giardia spp. in Mussels (Mytilus californianus) and California Sea Lions (Zalophus californianus) from Central California." Applied and Environmental Microbiology 80, no. 24 (2014): 7732–40. http://dx.doi.org/10.1128/aem.02922-14.

Full text

Abstract:

ABSTRACTCryptosporidiumandGiardiaare of public health importance, with recognized transmission through recreational waters. Therefore, both can contaminate marine waters and shellfish, with potential to infect marine mammals in nearshore ecosystems. A 2-year study was conducted to evaluate the presence ofCryptosporidiumandGiardiain mussels located at two distinct coastal areas in California, namely, (i) land runoff plume sites and (ii) locations near sea lion haul-out sites, as well as in feces of California sea lions (CSL) (Zalophus californianus) by the use of direct fluorescent antibody (DF

APA, Harvard, Vancouver, ISO, and other styles

42

Grodin, J. L., K. S. Wu, E. E. Kitchell, et al. "Respiratory Syncytial Virus Pneumonia Treated with Lower-Dose Palivizumab in a Heart Transplant Recipient." Case Reports in Cardiology 2012 (2012): 1–4. http://dx.doi.org/10.1155/2012/723407.

Full text

Abstract:

Respiratory syncytial virus (RSV) is an important community-acquired pathogen that can cause significant morbidity and mortality in patients who have compromised pulmonary function, are elderly, or are immunosuppressed. This paper describes a 70-year-old man with a remote history of heart transplantation who presented with signs and symptoms of pneumonia. Chest computed tomography (CT) imaging demonstrated new patchy ground glass infiltrates throughout the upper and lower lobes of the left lung, and the RSV direct fluorescence antibody (DFA) was positive. The patient received aerosolized ribav

APA, Harvard, Vancouver, ISO, and other styles

43

Isara, Alphonsus, and Aru-Kumba Baldeh. "Prevalence of sexually transmitted infections among pregnant women attending antenatal clinics in West Coast Region of The Gambia." African Health Sciences 21, no. 2 (2021): 585–92. http://dx.doi.org/10.4314/ahs.v21i2.13.

Full text

Abstract:

Background: Sexually Transmitted Infections (STI) are the second most common cause of healthy life years lost by women in the 15 – 44 years age group in Africa. Aim/Objective: To determine the prevalence of STIs among pregnant women attending antenatal care (ANC) clinics in the West Coast Region of The Gambia. Materials and Methods: Blood, urine, and high vaginal swabs samples from 280 pregnant women attending ANC in Brika- ma District Hospital, Brikama, and Bandung Maternity and Child Health Hospital, Bandung were examined. Serum samples were tested for HIV using western blot tech

APA, Harvard, Vancouver, ISO, and other styles

44

Dionne, Brandon, Meghan Brett, Karissa Culbreath, and Renee-Claude Mercier. "Potential Ceiling Effect of Healthcare Worker Influenza Vaccination on the Incidence of Nosocomial Influenza Infection." Infection Control & Hospital Epidemiology 37, no. 7 (2016): 840–44. http://dx.doi.org/10.1017/ice.2016.77.

Full text

Abstract:

OBJECTIVETo evaluate the effect of healthcare worker (HCW) influenza vaccination on the incidence of nosocomial influenzaDESIGNRetrospective cross-sectional studySETTINGA 550-bed tertiary-care academic medical centerMETHODSAll admitted patients with a direct fluorescent antibody (DFA) or polymerase chain reaction (PCR) assay positive for influenza ordered between October 1 and May 31 from 2010 to 2015 were eligible for inclusion. Nosocomial influenza was defined as a positive influenza test collected ≥48 hours after admission in patients without influenza-like illness present within 24 hours o

APA, Harvard, Vancouver, ISO, and other styles

45

Titze, Nicole, Jasjit Singh, and Wendi Gornick. "2620. Respiratory Syncytial Virus Rapid Antigen Detection Test, Can It Be Trusted?" Open Forum Infectious Diseases 6, Supplement_2 (2019): S912. http://dx.doi.org/10.1093/ofid/ofz360.2298.

Full text

Abstract:

Abstract Background Many emergency departments and urgent care settings use the commonly available Respiratory Syncytial Virus Rapid Antigen Detection Test (RSV RADT) to diagnose children with RSV. We noted discordant results between RADT and definitive testing. Our study looked at the positive predictive value (PPV) and the false discovery rate (FDR) of the RSV RADT at our facility. Methods We pro- and retrospectively reviewed all patients with positive RSV RAPD tests from July 1, 2017 through March 31, 2019. The test utilized was the QuickVue® RSV Test Kit (QUIDEL Corp, CA, USA), which detec

APA, Harvard, Vancouver, ISO, and other styles

46

Ceccarelli, Daniela, Arlene Chen, Nur A. Hasan, Shah M. Rashed, Anwar Huq, and Rita R. Colwell. "Non-O1/Non-O139 Vibrio cholerae Carrying Multiple Virulence Factors and V. cholerae O1 in the Chesapeake Bay, Maryland." Applied and Environmental Microbiology 81, no. 6 (2015): 1909–18. http://dx.doi.org/10.1128/aem.03540-14.

Full text

Abstract:

ABSTRACTNon-O1/non-O139Vibrio choleraeinhabits estuarine and coastal waters globally, but its clinical significance has not been sufficiently investigated, despite the fact that it has been associated with septicemia and gastroenteritis. The emergence of virulent non-O1/non-O139V. choleraeis consistent with the recognition of new pathogenic variants worldwide. Oyster, sediment, and water samples were collected during a vibrio surveillance program carried out from 2009 to 2012 in the Chesapeake Bay, Maryland.V. choleraeO1 was detected by a direct fluorescent-antibody (DFA) assay but was not suc

APA, Harvard, Vancouver, ISO, and other styles

47

Ross, Justine Abella, Bernard Tegtmeier, Deron Johnson, et al. "36. Evaluation of a Best Practice Alert (BPA) to Improve Pneumocystis jirovecii Prophylaxis Prescribing in Cancer Patients Receiving High-dose Corticosteroids in an Outpatient Setting." Open Forum Infectious Diseases 8, Supplement_1 (2021): S140. http://dx.doi.org/10.1093/ofid/ofab466.238.

Full text

Abstract:

Abstract Background In patients (pts) with cancer, the risk of Pneumocystis jirovecii pneumonia (PJP) is a function of dose and duration of corticosteroids (CS), underlying immunodeficiency, and immunosuppressive drugs. Trimethoprim/sulfamethoxazole (TMP/SMX) and atovaquone (ATO) are effective prophylaxis (ppx) agents against PJP. Guidelines recommend PJP ppx for pts on &gt; 20 mg /day of prednisone or its equivalent for ≥ 1 month. A best practice alert (BPA) to identify pts receiving CS may assist with improving PJP ppx prescribing in cancer pts. Methods PJP BPA was created to identify pt

APA, Harvard, Vancouver, ISO, and other styles

48

Sultana, Marzia, Suraia Nusrin, Nur A. Hasan, et al. "Biofilms Comprise a Component of the Annual Cycle ofVibrio choleraein the Bay of Bengal Estuary." mBio 9, no. 2 (2018): e00483-18. http://dx.doi.org/10.1128/mbio.00483-18.

Full text

Abstract:

ABSTRACTVibrio cholerae, an estuarine bacterium, is the causative agent of cholera, a severe diarrheal disease that demonstrates seasonal incidence in Bangladesh. In an extensive study ofV. choleraeoccurrence in a natural aquatic environment, water and plankton samples were collected biweekly between December 2005 and November 2006 from Mathbaria, an estuarine village of Bangladesh near the mangrove forests of the Sundarbans. ToxigenicV. choleraeexhibited two seasonal growth peaks, one in spring (March to May) and another in autumn (September to November), corresponding to the two annual seaso

APA, Harvard, Vancouver, ISO, and other styles

49

Drelick, Alexander Christian, Priya Kodiyanplakkal, Markus Plate, et al. "2695. Pneumocystis jirovecii Pneumonia in the Era of Effective Prophylaxis Following Hematopoietic Stem Cell Transplant." Open Forum Infectious Diseases 6, Supplement_2 (2019): S947—S948. http://dx.doi.org/10.1093/ofid/ofz360.2372.

Full text

Abstract:

Abstract Background Pneumocystis jirovecii pneumonia is an uncommon and life-threatening disease that can occur following hematopoietic stem cell transplantation (HSCT). Trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis greatly reduces the incidence of PJP. We aim to determine what factors contribute to the development of PJP following HSCT where TMP-SMX prophylaxis is widely used. Methods We performed a single-center, retrospective case series of HSCT recipients from January 1, 2012 to December 31, 2018. Subjects had clinical symptoms and radiographic evidence for PJP along with at least on

APA, Harvard, Vancouver, ISO, and other styles

50

Ayala-Lopez, Nadia, Mahboobe Ghaedi, David Peaper, and Roa Harb. "Performance of the Procalcitonin Test in Diagnosing Pneumonia in Real-World Practice." American Journal of Clinical Pathology 152, Supplement_1 (2019): S36. http://dx.doi.org/10.1093/ajcp/aqz112.069.

Full text

Abstract:

Abstract Background Procalcitonin (PCT) rises early upon bacterial infection and has a long-half life, making it useful in the diagnosis of infections and antibiotic stewardship. We sought to determine the utility of PCT in diagnosing pneumonia (PNU) in the patient population presenting to our hospital during real-world clinical practice. Methods PCT results from patients in 2015 were reviewed retrospectively. Patients were eligible for inclusion if all of following criteria were met: PCT in 2015 and a lower respiratory tract culture, respiratory virus testing, chest x-ray, and white blood cel

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!