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Dissertations / Theses on the topic 'Direct nucleic acid detection'

Author: Grafiati

Published: 11 March 2023

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1

Lores, Lareo Pablo. "Nucleic acids and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/20133.

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In den letzten Jahren gab es rasche Weiterentwicklungen auf dem Gebiet der Nukleinsäure-Erkennung. Von microRNA-Quantifizierung zur Untersuchung von Zelltods, --Teilung und -Regulation bis zur Bewertung genetischer Variabilität in Hinblick auf Krankheitsentstehung und -Behandlung: Die Analyse von Nukleinsäuren wird in der zukünftigen Medizin eine zentrale Rolle zukommen. Vor allem die Erkennung von SNPs als Hauptquelle der genetischen Vielfalt, aber aus Analysesicht auch eine der herausforderndsten Mutationen, stellt in dieser Hinsicht einen wesentlichen Aspekt dar. Methoden zur SNP-Erkennung

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2

Chatwell, Nicola. "Nucleic acid approaches to toxin detection." Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606582.

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PCR is commonly used for detecting contamination of foods by toxigenic bacteria. However, it is unknown whether it is suitable for detecting toxins in samples which are unlikely to contain bacterial cells, such as purified biological weapons. Quantitative real-time PCR assays were developed for amplification of the genes encoding Clostridium botulinum neurotoxins A to F, Staphylococcal enteroxin B (SEB), ricin, and C. perfringens alpha toxin. Botulinum neurotoxins, alpha toxin, ricin and V antigen from Yersinia pestis were purified at Dstl using methods including precipitation, ion exchange, F

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3

Behrmann, Ole [Verfasser], and Gerald A. [Akademischer Betreuer] Urban. "Methods for rapid nucleic acid extraction and detection." Freiburg : Universität, 2021. http://d-nb.info/1227187289/34.

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4

Ferrier, David Christopher. "Nucleic acid detection using oligonucleotide cross-linked polymer composites." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28944.

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There has been much interest in recent years about the potential of microRNA as a new source of biomarkers for the diagnosis of disease. The delivery of new diagnostic tools based on this potential has been limited by shortcomings in current microRNA detection techniques. This thesis explores the development of a new method of microRNA detection through the incorporation of conductive particles into oligonucleotide-functionalised polymers to form oligonucleotide cross-linked polymer composites. Such composites could provide a simple, rapid, and low-cost means of microRNA detection that could b

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5

Gorgannezhad, Lena. "Advanced Technologies in Rapid and Multiplex Detection of Nucleic acid." Thesis, Griffith University, 2020. http://hdl.handle.net/10072/397045.

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Nucleic acids are key macromolecules of living organisms transferring genetic inheritance from one generation to the next. From how a living individual is created to how it interacts with external factors, all and all, can be found in the nucleic acid sequences inside every single cell of every organism. Therefore, the analysis of nucleic acids sequences is a critical capability for cancer and pathogen diagnoses, genotyping, and disease monitoring. To date, numerous methods have been used to detect both characterised and uncharacterised mutations and sequence variations. However, the detection

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6

Kershaw, David Michael. "Nanoparticle bound nucleic acid probes for DNA detection and gene inactivation." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7432/.

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In this project, a gold nanoparticle system has been developed that is able to detect SNP variations through a DNA based anthracene probe. A second probe is bound to the gold nanoparticle which allows the fluorescent output of the anthracene probe to be normalized. This allows the detection of SNP variations without the need for an initial reading, opening the possibility for using this system for cellular SNP identification. Through this work a new method for coating gold nanoparticles in oligonucleotides has been developed. In further work, the use of gold nanoparticles to deliver siRNA into

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7

Saeed, Ibrahim Q. "Optoelectronically active sensitisers for the selective detection of nucleic acid biomarkers." Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/100885/.

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This thesis presents biophysical studies of new optoelecronically active DNA-binders. Chapter one gives a brief overview of the importance of DNA in medicine, of DNA structure and of the mode of interactions of small molecules with double-stranded DNA, including electrostatic, intercalation and groove interactions. Various examples of small-molecule binding to DNA are discussed. Additionally, this chapter briefly describes the biophysical techniques which can be exploited to quantify the interaction between small-molecules and duplex DNA. Chapter two describes the results of studies of the int

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8

O'Meara, Deirdre. "Molecular Tools for Nucleic Acid Analysis." Doctoral thesis, Stockholm : Tekniska högsk, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3220.

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9

Khater, Mohga Wagdy Yehia Mohamed. "Nanoparticle-based sensors for pathogen nucleic acid detection with interest for agriculture." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667373.

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La presente tesis describe el desarrollo de sensibles, bajo coste y portátiles métodos de sensado basados en nanomateriales aplicados en la detección de ADN de patógenos relacionados a plantas. El trabajo se presenta avances significativos en el campo de los biosensores para el diagnóstico de las enfermedades en las plantas. En el Capítulo I se da una visión general de las aplicaciones llevabas a cabo y mejoras aportadas por parte del uso de nanomateriales diferentes en el campo de los biosensores, y las nuevas aplicaciones en la detección de enfermedad de las plantas en lo punto de atención.

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10

Baloda, Meenu. "Lateral Flow Nucleic Acid Biosensor for the Detection of Sexually Transmitted Diseases." Diss., North Dakota State University, 2015. https://hdl.handle.net/10365/27596.

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Nucleic acid detection is of central importance for the diagnosis and treatment of genetic diseases, infectious agents, bio-warfare agents, and drug discovery. Nucleic acid testing for diseases is exclusively performed in laboratories using high-end instrumentation and personnel. However, this has developed the need for point of care diagnostics which can provide near-patient testing in a clinic, doctor’s office, or home. Such diagnostic tools can prove advantageous when rapid response is required or when suitable facilities are unavailable. Compared to equivalent methods used in laboratories,

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11

Stains, Cliff. "Methods for the Detection of Protein-Nucleic Acid and Protein-Protein Interactions." Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/194834.

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We describe the first general approach for the DNA templated reassembly of proteins, which we term SEquence-Enabled Reassembly or SEER. SEER makes use of dissected signaling domains which are each attached to separate, sequence specific DNA-binding proteins. Described herein is an embodiment of SEER in which DNA catalyzes the reassembly of the green fluorescent protein which leads to a direct fluorescence readout of the corresponding DNA sequence. This strategy has also been extended to the first direct method for the site specific detection of DNA methylation. This mCpG-SEER system is cap

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12

Mokany, Elisa Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "The development of multi-component nucleic acid enzymes(MNAzymes)for the detection of analytes." Awarded by:University of New South Wales, 2007. http://handle.unsw.edu.au/1959.4/35210.

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13

Badran, Ahmed Hussein. "Split-Protein Systems for the Detection and Interrogation of Protein-Nucleic Acid Interactions." Thesis, The University of Arizona, 2010. http://hdl.handle.net/10150/146847.

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Cys2-His2 zinc fingers constitute one of the largest classes of DNA-binding domains in the human genome. The modularity of these domains has been recently exploited to design artificial zinc fingers, capable of targeting virtually any sequence. However, the resultant zinc fingers have had significantly high failure rate, owing to low binding affinity and selectivity. Despite much research on the topic, a proper understanding of all the factors involved in zinc finger selectivity, be they natural or artificial, has proved elusive. Here, we present a modification of our previously reported SEque

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14

Thomas, Alistair Owen. "Detection of bacterial gene expression by a novel isothermic nucleic acid amplification technology." Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410924.

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A novel probe-based technique called Signal-Mediated Amplification Reaction Technology (SMART) was optimised for detection of RNA targets in order to quantify gene expression. The SMART assay was used to quantify both 23 S rRNA in P. aeruginosa PAOl and gfpmuti mRNA in the plasmid-borne rpoSwgfpmvXi fusions P. aeruginosa SS429 and SS431. However, the assay was not sufficiently sensitive to detect gfpmvfo mRNA from the chromosomal rpoS::gfpmut3 fusion P. aeruginosa SS336. SDS-PAGE analysis of outer membrane proteins of P. aeruginosa PAOl revealed that cells grown in a reported iron-replete chem

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15

Gorbunova, Santa Maria. "Electrochemical characterization of carminic acid towards the use as an electrochemical molecular beacon for nucleic acid detection." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52894.

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Worldwide, more than a million people die from tuberculosis (TB) every year. Although the disease is curable, treatment is complicated by multi-drug resistant and extensively drug-resistant TB strains. To detect TB and differentiate between its strains, a sensitive and specific point-of-care device is required. Previous studies show that carminic acid (CA), an anthraquinone derivative, is suitable as an electrochemical molecular beacon due to the ability to switch on and off its electrochemical activity on its dimerization. Characterization of the electrochemical activity of CA at low concent

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16

Periyannan, Rajeswari Prem Kumar. "Droplet microfluidics for single cell and nucleic acid analysis." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-192668.

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Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. By utilizing the unit operations developed to generate, manipulate and analyze droplets, this technology platform has been used to miniaturize a wide range of complex biological assays including, but not limited to, directed evolution, rare cell detection, single cell transcr

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17

Zozulia, Oleksii [Verfasser], and Andriy [Gutachter] Mokhir. "Red light-triggered nucleic acid-templated reactions for detection of nucleic acids in live cells / Oleksii Zozulia ; Gutachter: Andriy Mokhir." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2017. http://d-nb.info/1135779791/34.

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18

Tomlinson, Jennifer A. "Nucleic acid-based methods for on-site detection of plant pathogens : approaches and applications." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12957/.

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The ability to perform nucleic acid-based detection of plant pathogens away from conventional laboratory facilities has the potential to be beneficial in situations where results are required very rapidly or where resources and access to laboratory equipment are limited. Methods for use in such situations must combine sensitivity and specificity with rapid and simple workflows. The aim of this project was to investigate aspects of on-site testing for plant pathogens by developing detection methods for a range of target species. Detection methods based on loop-mediated isothermal amplification

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19

Xiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.

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20

Derbyshire, Nicola. "Exploring the application of nucleic acid aptamers for detection of food contaminants and viruses." Thesis, University of Leeds, 2012. http://etheses.whiterose.ac.uk/4136/.

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Use of antibiotic, anti-fungal and anti-parasitic agents is required to protect from infections that can rapidly spread making entire crops unsuitable for human consumption or threaten the health of livestock. However, these agents can have toxic side effects on humans at high concentrations so their residual levels in food are regulated. Their levels are monitored through both random and routine surveillance checks that generate ~ 50 000 samples per year in the UK alone. This is not an easy task because many of these compounds are small molecules that require extensive sample extraction follo

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21

Hernández-Neuta, Iván. "Nucleic acid analysis tools : Novel technologies and biomedical applications." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-146334.

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Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. Therefore, accessing these sequences provides key information regarding the molecular identity and functional state of any living organism, this is very useful for areas like biomedicine, where accessing and understanding these molecular signatures is the key to develop strategie

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22

Metcalf, Gavin Alan Dorman. "Fluorogenic Peptide Nucleic Acid probes for the detection of circulating microRNAs : applications to cancer diagnosis." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/45000.

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Cancer is a global burden with escalating incidence and mortality, often as a result of late diagnosis. Therefore, early detection, in order to improve prognosis and survival, remains a vital strategy for cancer management. A promising method for early diagnosis requires highly sensitive biomarkers and suitable technologies to detect them. Highly abundant in cells, microRNAs (miRNAs or miRs) play a key role as regulators of gene expression. A proportion of them are found circulating in biofluids, making them ideal non-invasive biomarkers for pathologies which aberrantly express miRNAs, such as

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23

Tsourkas, Andrew. "Development and optimization of dual FRET-molecular beacons for the detection and visualization of single-stranded nucleic acid targets." Diss., Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/19256.

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24

Spencer, Sarah M. "Development of RNA Microchip for the Detection of Pathogens." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/chemistry_diss/35.

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Detection of cellular messenger RNA is a useful diagnostic strategy for the detection of patho-gens. A rapid and sensitive method for on-site detection of specific pathogens would be of great use in a number of fields. For example, a simple and inexpensive method for the detection of harmful biological agents in train stations and airports is useful for national security. Rapid detection of pathogenic E. coli strains in food production would also be of great benefit in ensuring the safety and quality of our food supply. Here we present a method for the rapid de-tection of cellular mRNA. This s

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25

Grant, Paul Robert. "Development and application of nucleic acid amplification technology (NAT) for the detection of viruses in donated blood." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408734.

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26

Richardson, Kenneth James. "Use of nucleic acid probes and a nonradioactive labeling system for the detection of enteroviruses in water." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184948.

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Enteroviruses affect a broad segment of the population throughout the world and have been suspected to play a major role in waterborne disease for quite some time. The presence of these viruses in drinking water supplies constitutes a major health risk to the population because of their low infectious dose. The monitoring and study of these viruses in the environment have been limited by the current standard detection methodologies. Nucleic acid probe hybridization is a new and effective approach for the study and detection of these viruses in the environment. An important step in the detectio

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27

Sailis, Fiammetta. "Detection of miRNA by SMART technology." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28891.

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Aberrant expression of short non-coding micro RNAs (miRNA) in many human diseases, along with remarkable stability in physiological media, has made them attractive clinical biomarkers. In particular, miRNA-122 is substantially elevated in plasma of patients with established drug-induced liver injury and can also be used to identify early liver injury when current markers, such as alanine aminotransferase (ALT), still show normal levels. The development of a rapid test for miRNA-122 e.g. in drug poisoning would allow earlier and more sensitive clinical diagnosis of liver injury. Nucleic acids a

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28

Kerr, Samantha Louise. "Enhancing nucleic acid detection using inductively coupled plasma mass spectrometry, by means of metal and nano-particle labelling." Thesis, Loughborough University, 2008. https://dspace.lboro.ac.uk/2134/4641.

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The application of ICP-MS to the fields of proteomics and genomics has arisen in part due to its ability to detect and quantify trace levels of S and P, which are major constituents in proteins and nucleic acids respectively. The development of collision/reaction cell technology and high resolution instruments has enabled these biologically important elements to be measured and quantified at the pg - ng ml-1 level. Despite these advances, the detection limits of P and S are still inferior compared to other elements. Oligonucleotides containing biotin functionality were labelled with Au nano-pa

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29

Fredriksson, Simon. "Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders." Doctoral thesis, Uppsala University, Department of Genetics and Pathology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2691.

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<p>A novel technology for protein detection, proximity ligation, has been developed along with improved methods for <i>in situ</i> synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. As pairs of so-called proximity probes bind the individual target protein molecules at distinct sites, these reagents are brought in close proximity. The probes consist of a protein specific binding part coupled to an oligonucleotide with either a free 3’- or 5’-end capable of hybridizing to a common connec

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30

Zhang, Zhiling. "The structure and function of troponin T upon metal ion binding and the detection of nucleic acid sequence variations." Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc5565/.

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Numerous troponin T (TnT) isoforms are generated by alternative RNA splicing primarily in its NH2-terminal hypervariable region, but the functions of these isoforms are not completely understood. In this dissertation work, calcium and terbium binding behavior of several forms of TnT were investigated by spectroscopic and radioactive techniques. Chicken breast muscle TnT binds calcium and terbium through its NH2-terminal Tx motif (HEEAH)n with high affinity (10-6 mM) and fast on-rate (106 - 107 M-1 s-1). Chicken leg muscle TnT and a human cardiac TnT NH2-terminal fragment, which both lack the T

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31

Tang, Yeuk-nam Kennie. "A comparison of DIG nonradioactive with 32p radioactive nucleic acid labeling of Southern blot for the detection of alpha thalassaemia /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B32037661.

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32

Tang, Yeuk-nam Kennie, and 鄧若楠. "A comparison of DIG nonradioactive with 32p radioactive nucleic acid labeling of Southern blot for the detection of alpha thalassaemia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B45010456.

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33

Silverman, Adam Phillip. "Part I: Detection of RNA in cells with quenched autoligation probes ; probing active site tightness in nucleic acid replication enzymes /." May be available electronically:, 2007. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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34

Bernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.

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35

Nicolini, Ariana Marie, and Ariana Marie Nicolini. "Single-Step, Optical Biosensors for the Rapid and Sensitive Detection of Bacterial and Viral Pathogens." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/623158.

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This dissertation discusses the development of inexpensive, easy-to-use, and field-deployable diagnostic techniques and devices for the early detection of various pathogens, commonly found in clinical samples and contaminated food and water. Infectious diseases account for about 90% of world health problems, killing approximately 14 million people annually, the majority of which reside in developing countries. In 2012, the World Health Organization (WHO) published data on the top 10 causes of death across the globe. Although communicable disease is a prevalent cause of fatality, both low-incom

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36

Fun, Sze-tat. "Development of molecular diagnostic system for detection of hepatitis B virus in blood donations." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971751.

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37

Pinto, Alessandro. "Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/80744.

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The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. As compared to other natural and synthetic receptor, aptamers possess unique chemical and biochemical characteristics, such as: a well known chemistry, remarkable stability, an abi

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38

Nicholson, Wendy Jane. "The application of the polymerase chain reaction to the detection and characterization of human immunodeficiency virus and hepatitis B virus nucleic acid." Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/21446.

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This thesis considers the application of PCR to the detection and characterisation of human immunodeficiency virus (HIV) nucleic acid and hepatitis B virus (HBV) DNA in clinical samples. Primers were selected from the <I>po1 </I>and <I>env </I>sequences for the detection of HIV-1 DNA, and amplifications of the envelope gene were used to demonstrate sequence variability. HIV-1 DNA was detected in all 32 patients (59 of the 61 PBMC samples tested). Viral DNA sequences were detected in nuclear extracts tested, 71% of cytoplasmic extracts, and in 50% of purified monocytic cells. Primers were selec

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McCauley, Sean Matthew. "Innate Detection of HIV-1 in Myeloid Dendritic Cells." eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/993.

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Protective antiviral immune responses require priming of naïve T cells by dendritic cells (DCs) that have matured sufficiently to produce co-stimulatory cell surface molecules and cytokines. Although only low levels of productive HIV-1 infection are detected in ex vivo DCs following HIV-1 challenge, those few cells exhibit innate activation. Experimentally bypassing blocks to entry and replication leads to more efficient transduction of DCs and maturation as indicated by production of interferons and interferon stimulated genes. Furthermore, similar innate activation occurs upon transduction o

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Weldhagen, Gerhard Frederick. "Laboratory Detection and Gene Cassette Stability of the Novel Extended-Spectrum Beta-Lactamase, GES-2 from Pseudomonas aeruginosa." Thesis, University of Pretoria, 2004. http://hdl.handle.net/2263/29221.

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Extended-spectrum beta-lactamases (ESBLs) in Pseudomonas aeruginosa tend to be geographically scattered, such as GES-2, which partially compromises the efficacy of imipenem. The G170N mutation, ascribed to a CC to AA base pair substitution on positions 493-494 of the blaGES-2 coding region, distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for developing a novel sequence-specific, peptide nucleic acid (PNA)-based, multiplex-PCR detection method. Utilizing two primer pairs in conjunction with a PNA probe, this novel method delivered accurate identificati

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Porter, Jason Robert. "SPLIT-PROTEIN REASSEMBLY METHODS FOR THE DETECTION AND INTERROGATION OF BIOMOLECULAR INTERACTIONS AND MODULATORS THEREOF." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/194359.

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The interactions between protein-protein, protein-nucleic acid, and protein-small molecules are central to biological processes and are key for the design of new therapeutics. Rapid and easy to implement methodologies are needed that enable the interrogation of these interactions in a complex cellular context. Towards this goal, I have utilized the concept of split-protein reassembly, also called protein complementation, for the creation of a variety of sensor architectures that enable the interrogation of protein-nucleic acid, protein-protein, and protein-small molecule interactions. Utilizin

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42

Lehnus, Massimiliano. "Bio-BCA (Bio-Barcode Cascade Amplification) : development of a photosensitive, DNA-based exponential amplification platform technology for the detection of nucleic acid biomarkers." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277915.

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Seitz, Oliver [Gutachter], Michael W. [Gutachter] Linscheid, and Ilko [Gutachter] Bald. "Nucleic acids and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry / Gutachter: Oliver Seitz, Michael W. Linscheid, Ilko Bald." Berlin : Humboldt-Universität zu Berlin, 2019. http://d-nb.info/119993061X/34.

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Mantha, Stacey. "Direct detection and rapid identification of clinically significant mycobacteria from Bactec 12B medium and acid-fast bacilli positive sputum specimens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0015/MQ54472.pdf.

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Lam, Yiu-pong, and 林耀邦. "Performance evaluation of the automated NucliSens easyMAG and Qiagen EZ1 Advanced XL nucleic acid extraction platform for detection of RNAand DNA viruses in clinical samples." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46448020.

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46

Fun, Sze-tat, and 范思達. "Development of molecular diagnostic system for detection of hepatitis B virus in blood donations." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971751.

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Faltin, Bernd [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Mediator Probe PCR: a novel assay principle for universal real-time detection of nucleic acid amplification = Mediator Probe PCR: ein neuartiger Ansatz zur universellen Echtzeit-Detektion von Nukleinsäuren." Freiburg : Universität, 2013. http://d-nb.info/1123477000/34.

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Ceylan, Koydemir Hatice. "Mems Based Electrochemical Dna Sensor To Detect Methicillin Resistant Staphylococcus Aureus And Vancomycin Resistant Enterococcus Species." Phd thesis, METU, 2013. http://etd.lib.metu.edu.tr/upload/12615402/index.pdf.

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Methicillin Resistant Staphylococcus aureus (MRSA) is one of the most important threats of nosocomial infections in many regions of the world and Vancomycin Resistant Enterococcus (VRE) is an emerging pathogen that develops full resistance against third-generation glycopeptide antibiotics. Conventional methods for identification of MRSA and VRE generally depend on culturing, which requires incubation of biological samples at least 24-72 hours to get accurate results. These methods are time consuming and necessitate optical devices and experts for evaluation of the results. On the other hand

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VERMA, SUJIT KUMAR [Verfasser], Sebastian [Akademischer Betreuer] Springer, Sebastian [Gutachter] Springer, Michael [Gutachter] Köhler, Mathias [Gutachter] Winterhalter, and Werner M. [Gutachter] Nau. "Polyelectrolyte Microcapsules: A versatile and sensitive tool for the detection of protein and nucleic-acid analytes / SUJIT KUMAR VERMA ; Gutachter: SEBASTIAN SPRINGER, MICHAEL KÖHLER, MATHIAS WINTERHALTER, WERNER M. NAU ; Betreuer: SEBASTIAN SPRINGER." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2017. http://d-nb.info/114810397X/34.

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Fischbach, Melanie. "Haarnadelförmige PNA-Peptid-Konjugate." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17305.

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Das Entwicklungsstadium bestimmter Krankheiten ist eng mit der Konzentration diverser Proteine in biologischen Proben verknüpft. Eine sensitive Detektion dieser sogenannten Biomarker kann somit maßgeblich zu einer frühzeitigen Diagnose beitragen. In der vorliegenden Arbeit wurden strukturierte, fluorogene Sonden entwickelt, die die Möglichkeit bieten in einem homogenen Verfahren Zielproteine sensitiv, direkt und in Echtzeit nachzuweisen. Die peptidische Erkennungssequenz für das Zielprotein wurde dabei von zwei zueinander komplementären PNA-Segmenten flankiert. Die Sonden besaßen dadurch eine

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