Relevant bibliographies by topics / Direct sequencing

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Direct sequencing'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 March 2023

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Journal articles on the topic "Direct sequencing"

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Ozsolak, Fatih, Adam R. Platt, Dan R. Jones, Jeffrey G. Reifenberger, Lauryn E. Sass, Peter McInerney, John F. Thompson, Jayson Bowers, Mirna Jarosz, and Patrice M. Milos. "Direct RNA sequencing." Nature 461, no. 7265 (September 23, 2009): 814–18. http://dx.doi.org/10.1038/nature08390.

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Linder, Jodell E., Tatyana E. Plachco, Romina Libster, and E. Kathryn Miller. "Sequencing human rhinoviruses: Direct sequencing versus plasmid cloning." Journal of Virological Methods 211 (January 2015): 64–69. http://dx.doi.org/10.1016/j.jviromet.2014.09.020.

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Chalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (August 24, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.2.

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Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand. Direct sequencing of single-stranded nucleic acid species is challenging as they are non-compatible to the double-stranded sequencing adapters used by manufacturers. The MinION platform from Oxford Nanopore Technologies performs sequencing by passing single-strands of DNA through
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Chalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (May 12, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.1.

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Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand. Direct sequencing of single-stranded nucleic acid species is challenging as they are non-compatible to the double-stranded sequencing adapters used by manufacturers. The MinION platform from Oxford Nanopore Technologies performs sequencing by passing single-strands of DNA through
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Rudi, Heidi, Knut‐Erik Gylder, Odd Arne Rognli, and Knut Rudi. "Direct Haplotype‐Specific DNA Sequencing." Preparative Biochemistry and Biotechnology 36, no. 3 (September 2006): 253–57. http://dx.doi.org/10.1080/10826060600716687.

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Zhang, Jinyue, Shuanghong Yan, Le Chang, Weiming Guo, Yuqin Wang, Yu Wang, Panke Zhang, Hong-Yuan Chen, and Shuo Huang. "Direct microRNA Sequencing Using Nanopore-Induced Phase-Shift Sequencing." iScience 23, no. 3 (March 2020): 100916. http://dx.doi.org/10.1016/j.isci.2020.100916.

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Kilger, Christian, Matthias Krings, Hendrik Poinar, and Svante Pääbo. "“Colony Sequencing”: Direct Sequencing of Plasmid DNA from Bacterial Colonies." BioTechniques 22, no. 3 (March 1997): 412–18. http://dx.doi.org/10.2144/97223bm08.

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Petry, H., K. Pekrun, K. Wäse, I. Schedel, W. Lüke, and G. Hunsmann. "Direct sequencing versus cloned amplicon sequencing in HIV-1 diagnosis." Experientia 52, no. 4 (April 1996): 303–4. http://dx.doi.org/10.1007/bf01919520.

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Obata, Hiroko, Tatsuya Tanaka, Tsuneko Fujii, Chikako Sasho, Yukihiro Yamaguchi, and Keiichiro Suzuki. "Dye Terminator Re-cycle-sequencing Method: Phage Plaque Direct Sequencing." Analytical Biochemistry 297, no. 1 (October 2001): 102–5. http://dx.doi.org/10.1006/abio.2001.5328.

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Zhang, Jinyue. "Direct MicroRNA Sequencing using Nanopore Induced Phase-Shift Sequencing (NIPSS)." Biophysical Journal 118, no. 3 (February 2020): 475a—476a. http://dx.doi.org/10.1016/j.bpj.2019.11.2638.

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Dissertations / Theses on the topic "Direct sequencing"

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Fillion, Bergeron Marianne. "Plasmid isolation and purification by electrofiltration and comparison of different direct colony sequencing methods and PCR-based sequencing methods." Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6050.

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We have designed an electrophoresis system that can purify plasmid DNA from a culture without centrifugation. This system is based on electrofiltration where bacterial cell lysates are loaded in one chamber and the purified plasmid DNA is recovered in an adjacent chamber. These two chambers are separated by a membrane made of regenerated cellulose, which allows plasmid DNA to migrate to the recovery chamber while retaining most contaminants in the loading chamber. Unfortunately, even with the optimization of the parameters involved in the electrofiltration, the only DNA that can pass through t
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梁秀敏 and Sau-man Leung. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970102.

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Leung, Sau-man. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2329498X.

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Otten, Nathalie. "DNA of bovine papillomavirus type 1 and 2 in equine sarcoids : PCR detection and direct sequencing /." [S.l.] : [s.n.], 1992. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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Rocha, Ana Laís Bignotto da. "Sequenciamento direto dos genes SIX3, SHH, TGIF1, ZIC2 e array-CGH no estudo de pacientes com holoprosencefalia." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-12112013-150520/.

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Objetivos: Analisar por meio da técnica de sequenciamento direto a presença de alterações moleculares nos genes SHH, SIX3, ZIC2 e TGIF1 em indivíduos com diagnóstico clínico de HPE. Analisar por meio da técnica de CGH-array a presença de alterações moleculares em indivíduos com diagnóstico clínico de HPE previamente submetidos à análise por sequenciamento direto. Local: Laboratório de Genética e Citogenética Humana HRAC/USP, Bauru-SP. Casuística e metodologia: Foram selecionados 50 indivíduos, de ambos os sexos com idades entre 03 meses a 50 anos com diagnóstico clínico para HPE. Todos foram a
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Vander, Wiede Jamie. "Direct, hands-on or inquiry instruction a study of instructional sequencing and motivation in the science classroom." Master's thesis, University of Central Florida, 2011. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5073.

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Currently, a debate exists between the strengths and weaknesses of direct and inquiry instruction. Inquiry instruction is related to positive effect on learner motivation whereas supporters of direct instruction point to its ability to adequately support learners' working memories (Hmelo-Silver, Duncan, & Chinn, 2007; Kirschner, Sweller, & Clark, 2006; Kuhn, 2007; Sweller, 1988). This study examined the possibility of combining the best features of both inquiry and direct instruction by sequencing them together. A two-part lesson on electrical circuits was presented in three separate sequences
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Koziol, Adam. "Application of Direct-sequencing Peptide Proteomics to the Characterization of Antagonistic (Endogenous and Exogenous) Proteins in Cereal Grains." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/23853.

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The cereal seed plays a crucial role in society – both in the “food as medicine” paradigm, but also in food security. It is the starch and proteins present in the seed that lend it importance in these dissimilar anthropomorphic activities. This thesis investigation first characterized the post-translational processing of the potential diabetogen, wheat globulin-3. Globulin-3-like peptides were observed primarily in the embryo. These peptides varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting. Five major po
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Nguyen, Thuy. "Ultra-deep sequencing applications in virology research." Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS282.

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Les deux virus d’ARN VIH et VHC attirent beaucoup d’attention de la santé publique parce qu’ils partagent des mêmes facteurs de risque de transmission par le sang et par des contacts sexuels. En plus, les maladies associées aux infections par le VIH et par le VHC sont des causes principales de mortalité et de morbidité globalement. Néanmoins, grâce à l’introduction des thérapies d’antirétroviraux pour le traitement de l’infection par le VIH et les antiviraux à action directe (AADs) pour le traitement de l’infection par le VHC, les patients infectés par ces virus constatent une amélioration sig
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Engström, Erik. "Direct poly(A) RNA nanopore sequencing on the freshwater duck mussel Anodonta anatina following exposure to copper : A pilot study." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17508.

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Aquatic ecotoxicology is the study of toxic chemicals and its effects on aquatic biological systems with the aim of minimising threats to human health and ensure self-sustained ecosystems. Freshwater bivalves are excellent sentinels for use in ecotoxilogical research due to their filter feeding properties, stationary lifestyle and inability to regulate body temperature. This project aimed to assess the feasibility and use of nanopore sequencing, a real-time single-molecule sequencing technology in comparative expression analysis by sequencing transcriptomic RNA from the freshwater mussel Anodo
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Busson, Laurent. "Evolution of direct diagnostic techniques in Virology; analytical performances and clinical input." Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/313391.

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Le diagnostic virologique est un sujet d’actualité particulièrement du fait des récentes épidémiesou pandémies telles que la pandémie d’influenza A(H1N1) en 2009 ou la diffusion du virus Zika dansles Amériques et la région du Pacifique entre 2014 et 2017, associée à des cas de microcéphalie et dessyndromes de Guillain Barré. Encore plus récemment, en août 2018, le ministre de la santé de laRépublique Démocratique du Congo annonçait la 10e épidémie de virus Ebola dans le pays et endécembre 2019, le coronavirus SARS-CoV-2 est à l’origine d’une pandémie au départ de la Chine. Avecle nombre croiss
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Books on the topic "Direct sequencing"

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1937-, Jost J. P., ed. A laboratory guide to genomic sequencing: The direct sequencing of native uncloned DNA. Basel: Birkhäuser, 1987.

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Jost and Saluz. Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA. Birkhauser Verlag, 2013.

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Jost and Saluz. A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA. Birkhäuser, 2011.

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Jost, J. P., and H. P. Saluz. A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA (Biomethods). Birkhauser, 1988.

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Caillaud, Catherine, and Frédéric Sedel. Neuronal Ceroid Lipofuscinoses. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199972135.003.0059.

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Neuronal ceroid lipofuscinoses (NCLs) are inherited neurodegenerative disorders beginning mainly in childhood, rarely in adults. They are characterized by the accumulation of autofluorescent lipopigments in brain, especially in neurons. Their clinical heterogeneity is now explained by the huge number of genes (from CLN1 to CLN14) involved in their pathogenesis. Their diagnosis is possible using enzymatic tests and/or direct sequencing of the corresponding genes. Different therapeutic approaches are in development for these diseases such as enzyme replacement therapy or gene transfer.
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Ellingboe, James. The PCR Technique : DNA Sequencing. Eaton Publishing Company/Biotechniques Books, 1992.

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Graves, Tracey. Neurogenetic disease. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0223.

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There are many genetic diseases which affect the nervous system. Although some of these are extremely rare, several are quite common and, as a group, they comprise a significant proportion of neurological disease. Almost all clinical neurological syndromes can have a genetic cause. Not all of these have been genetically elucidated, but some have been extensively characterized in terms of clinical phenotype, molecular genetics, and cellular pathophysiology. Given the improvement in laboratory techniques and subsequent reduction in the cost of direct DNA sequencing, there is likely to be a rapid
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Harms, Matthew B., and Timothy M. Miller. Amyotrophic Lateral Sclerosis. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199937837.003.0027.

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Recent advances in sequencing technologies have dramatically expanded the number of genes associated with amyotrophic lateral sclerosis, including rare but highly penetrant causative mutations as well as common risk alleles. This chapter discusses these gene discoveries and how they have implicated a diverse array of biological pathways essential for motor neuron health and have begun to inform our understanding of ALS pathogenesis as a heterogeneous and multistep process. Insights from these discoveries are leading to a new generation of targeted therapies directed at specific genes and are p
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Vaheri, Antti, James N. Mills, Christina F. Spiropoulou, and Brian Hjelle. Hantaviruses. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0035.

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Hantaviruses (genus Hantavirus, family Bunyaviridae) are rodent- and insectivore-borne zoonotic viruses. Several hantaviruses are human pathogens, some with 10-35% mortality, and cause two diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus cardiopulmonary syndrome (HCPS) in the Americas. Hantaviruses are enveloped and have a three-segmented, single-stranded, negative-sense RNA genome. The L gene encodes an RNA-dependent RNA polymerase, the M gene encodes two glycoproteins (Gn and Gc), and the S gene encodes a nucleocapsid protein. In addition, the S genes of some
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Book chapters on the topic "Direct sequencing"

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Schieltz, David M., and John R. Yates. "Direct Identification of Proteins in Ultracomplex Mixtures." In Protein Sequencing Protocols, 235–45. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1385/1-59259-342-9:235.

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Ray, Kunal, Arijit Mukhopadhyay, and Mainak Sengupta. "Gene Discovery by Direct Genome Sequencing." In Gene Discovery for Disease Models, 215–33. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470933947.ch11.

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Mazars, Georges-Raoul, and Charles Theillet. "Direct Sequencing by Thermal Asymmetric PCR." In PCR Protocols, 355–59. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_52.

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Smith, Linda K. "HLA Typing by Direct DNA Sequencing." In Methods in Molecular Biology™, 67–86. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-842-9_5.

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Hong, Tao. "Direct Nucleotide Sequencing for Amplification Product Identification." In Advanced Techniques in Diagnostic Microbiology, 389–402. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-33900-9_19.

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Džunková, Mária. "Flow Cytometry and Direct Sequencing of Viruses." In The Human Virome, 3–14. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8682-8_1.

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Hong, Tao. "Direct Nucleotide Sequencing for Amplification Product Identification." In Advanced Techniques in Diagnostic Microbiology, 383–95. Boston, MA: Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-3970-7_22.

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Krautwurst, Sebastian, Ronald Dijkman, Volker Thiel, Andi Krumbholz, and Manja Marz. "Direct RNA Sequencing for Complete Viral Genomes." In Virus Bioinformatics, 35–50. Boca Raton: Chapman and Hall/CRC, 2021. http://dx.doi.org/10.1201/9781003097679-3.

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Hansen, C., M. Jørgensen, M. Bévort, R. Hummel, M. Løfgreen, N. Pallisgaard, and H. Leffers. "Direct Automated Sequencing of DDRT-PCR Fragments." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 345–51. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_35.

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Gyllensten, Ulf. "Direct Sequencing of In Vitro Amplified DNA." In PCR Technology, 45–60. London: Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-20235-5_5.

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Conference papers on the topic "Direct sequencing"

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Heath, Samuel A., Franco P. Preparata, and Joel Young. "Sequencing by hybridization using direct and reverse cooperating spectra." In the sixth annual international conference. New York, New York, USA: ACM Press, 2002. http://dx.doi.org/10.1145/565196.565220.

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Yanagi, Itaru, Rena Akahori, Tomio Iwasaki, Yusuke Goto, Kazuma Matsui, Yoshikazu Nara, Naomi Manri, Mayu Aoki, Takahide Yokoi, and Ken-ichi Takeda. "Fabrication and analysis of SiN nanopores for direct DNA sequencing." In 2015 IEEE International Electron Devices Meeting (IEDM). IEEE, 2015. http://dx.doi.org/10.1109/iedm.2015.7409692.

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Cox, M., S. Carney, L. Hoyles, S. Roy, R. Williams, J. Breuer, V. Chalker, and M. Moffatt. "Legionella Pneumophila Typing by Direct Sequencing of Clinical and Environmental Samples." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2121.

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Schmitt, Michael W., Jesse J. Salk, Edward J. Fox, Justin R. Pritchard, J. Graeme Hodgson, Victor M. Rivera, Pamela S. Becker, Jerald P. Radich, and Lawrence A. Loeb. "Abstract 2395: Direct assessment of sequence heterogeneity in human cancers by Duplex Sequencing." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-2395.

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Kolodziejski, Noah J., Rajan Gurjar, Karthik Vishwanath, and David Wolf. "Tip-Enhanced Raman Spectroscopy: Focusing in on a Direct Sequencing Method for Oxidized DNA." In Biomedical Optics. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/biomed.2014.bt3a.29.

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Yanagi, Itaru, Takeshi Oura, Takanobu Haga, Masahiko Ando, Jiro Yamamoto, Toshiyuki Mine, Takeshi Ishida, et al. "A novel side-gated ultrathin-channel nanopore FET (SGNAFET) sensor for direct DNA sequencing." In 2013 IEEE International Electron Devices Meeting (IEDM). IEEE, 2013. http://dx.doi.org/10.1109/iedm.2013.6724629.

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Sianipar, Nesti Fronika, Reflinur, Muhammad Dzulkifly Ashan, Khoirunnisa Assidqi, Dwityantari Widyaningrum, and Ragapadmi Purnamaningsih. "Direct verification of bogor superior mutant (Typhonium flagelliforme) using RAPD marker techniques and sequencing analysis." In THE 8TH INTERNATIONAL CONFERENCE AND WORKSHOP ON BASIC AND APPLIED SCIENCE (ICOWOBAS) 2021. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0104057.

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Karunyakorn, Jirachote, Naruemon Pratanwanich, Monnat Pongpanich, and Pornchai Kaewsapsak. "A Computational Workflow for Estimation of Short RNA Polyadenylation using Direct RNA Nanopore Sequencing with Polyuridylation." In 2022 19th International Joint Conference on Computer Science and Software Engineering (JCSSE). IEEE, 2022. http://dx.doi.org/10.1109/jcsse54890.2022.9836262.

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Flusberg, Benjamin, Dale Webster, Kevin Travers, Eric Olivares, Jonas Korlach, and Stephen Turner. "Abstract 1154: Direct detection of DNA methylation and mutagenic damage through single-molecule, real-time (SMRTTM) DNA sequencing." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-1154.

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Shekhar, Sudip, Rajesh Inti, James Jaussi, Tzu-Chien Hsueh, and Bryan Casper. "A 1.2–5Gb/s 1.4–2pJ/b serial link in 22nm CMOS with a direct data-sequencing blind oversampling CDR." In 2015 Symposium on VLSI Circuits. IEEE, 2015. http://dx.doi.org/10.1109/vlsic.2015.7231319.

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Reports on the topic "Direct sequencing"

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Rhodes, C. K. Human genome sequencing with direct x-ray holographic imaging. Office of Scientific and Technical Information (OSTI), June 1993. http://dx.doi.org/10.2172/6288788.

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Rhodes, C. K. Human genome sequencing with direct x-ray holographic imaging. Final report. Office of Scientific and Technical Information (OSTI), June 1993. http://dx.doi.org/10.2172/10168190.

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Gelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, December 2003. http://dx.doi.org/10.32747/2003.7586470.bard.

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Objectives 1. Determine the serotypic identities of 40 recent IBV isolates from commercial chickens raised in the USA and Israel. 2. Sequence all IBV field isolates using PCR product cycle sequencing and analyze their S 1 sequence to detennine their homology to other strains in the Genbank and EMBL databases. 3. Select vaccinal strains with the highest S 1 sequence homology to the field isolates and perform challenge of immunity studies in chickens in laboratory trials to detennine level of protection afforded by the vaccines. Background Infectious bronchitis (IB) is a common, economically imp
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Mehmood, Hamid, Surya Karthik Mukkavilli, Ingmar Weber, Atsushi Koshio, Chinaporn Meechaiya, Thanapon Piman, Kenneth Mubea, Cecilia Tortajada, Kimberly Mahadeo, and Danielle Liao. Strategic Foresight to Applications of Artificial Intelligence to Achieve Water-related Sustainable Development Goals. United Nations University Institute for Water, Environment and Health, April 2020. http://dx.doi.org/10.53328/lotc2968.

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The report recommends that: 1) Policymakers should conduct holistic assessments of social, economic, and cultural factors before AI adoption in the water sector, as prospective applications of AI are case- specific. It is also important to conduct baseline studies to measure the implementation capacity, return on investment, and impact of intervention. 2) To ensure positive development outcomes, policies regarding the use of AI for water-related challenges should be coupled with capacity and infrastructure development policies. Capacity development policies need to address the AI and Informati
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Fahima, Tzion, and Jorge Dubcovsky. Map-based cloning of the novel stripe rust resistance gene YrG303 and its use to engineer 1B chromosome with multiple beneficial traits. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598147.bard.

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Research problem: Bread wheat (Triticumaestivum) provides approximately 20% of the calories and proteins consumed by humankind. As the world population continues to increase, it is necessary to improve wheat yields, increase grain quality, and minimize the losses produced by biotic and abiotic stresses. Stripe rust, caused by Pucciniastriiformisf. sp. tritici(Pst), is one of the most destructive diseases of wheat. The new pathogen races are more virulent and aggressive than previous ones and have produced large economic losses. A rich source for stripe-rust resistance genes (Yr) was found in w
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Tang, Jiqin, Gong Zhang, Jinxiao Xing, Ying Yu, and Tao Han. Network Meta-analysis of Heat-clearing and Detoxifying Oral Liquid of Chinese Medicines in Treatment of Children’s Hand-foot-mouth Disease：a protocol for systematic review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, January 2022. http://dx.doi.org/10.37766/inplasy2022.1.0032.

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Review question / Objective: The type of study was clinical randomized controlled trial (RCT). The object of study is the patients with HFMD. There is no limit to gender and race. In the case of clear diagnosis standard, curative effect judgment standard and consistent baseline treatment, the experimental group was treated with pure oral liquid of traditional Chinese medicine(A: Fuganlin oral liquid, B: huangzhihua oral liquid, C: Lanqin oral liquid, D: antiviral oral liquid, E: Huangqin oral liquid, F: Pudilan oral liquid, G: Shuanghuanglian oral liquid.)and the control group was treated with
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Dubcovsky, Jorge, Tzion Fahima, Ann Blechl, and Phillip San Miguel. Validation of a candidate gene for increased grain protein content in wheat. United States Department of Agriculture, January 2007. http://dx.doi.org/10.32747/2007.7695857.bard.

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High Grain Protein Content (GPC) of wheat is important for improved nutritional value and industrial quality. However, selection for this trait is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. A gene with a large effect on GPC was detected on the short arm of chromosome 6B in a Triticum turgidum ssp. dicoccoides accession from Israel (DIC, hereafter). During the previous BARD project we constructed a half-million clones Bacterial Artificial Chromosome (BAC) library of tetraploid wheat including the high GPC allele from DIC and mapped the G
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Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.

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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA arra
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Mawassi, Munir, and Valerian V. Dolja. Role of the viral AlkB homologs in RNA repair. United States Department of Agriculture, June 2014. http://dx.doi.org/10.32747/2014.7594396.bard.

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AlkB proteins that repair DNA via reversing methylation damage are conserved in a broad range of prokaryotes and eukaryotes including plants. Surprisingly, AlkB-domains were discovered in the genomes of numerous plant positive-strand RNA viruses, majority of which belong to the family Flexiviridae. The major goal of this research was to reveal the AlkB functions in the viral infection cycle using a range of complementary genetic and biochemical approaches. Our hypotheses was that AlkB is required for efficient replication and genetic stability of viral RNA genomes The major objectives of the r
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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, January 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark
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