Relevant bibliographies by topics / Displacement amplification

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Academic literature on the topic 'Displacement amplification'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Dissertations / Theses on the topic "Displacement amplification"

1

Brown, Daniel F. Jr. "Single crystal piezoelectric pumping using displacement amplification." Thesis, Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/19124.

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2

Morant, Nick. "Novel thermostable DNA polymerases for isothermal DNA amplification." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667735.

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DNA polymerases play a fundamental role in the transmission and maintenance of genetic information and have become an important in vitro diagnostic and analytical tool. The Loop-mediated isothermal DNA amplification (LAMP) method has major applications for disease and pathogen detection and utilises the unique strand-displacement activity of a small group of thermostable DNA polymerases. The Large (Klenow-like) Fragment of Geobacillus stearothermophilus DNA polymerase I (B.st LF Pol I) currently serves as the enzyme of choice for the majority of these isothermal reactions, with few alternative
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Anscombe, C. J. "Multiple displacement amplification and whole genome sequencing for the diagnosis of infectious diseases." Thesis, Queen Mary, University of London, 2016. http://qmro.qmul.ac.uk/xmlui/handle/123456789/18409.

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Next-generation sequencing technologies are revolutionising our ability to characterise and investigate infectious diseases. Utilising the power of high throughput sequencing, this study reports, the development of a sensitive, non-PCR based, unbiased amplification method, which allows the rapid and accurate sequencing of multiple microbial pathogens directly from clinical samples. The method employs Φ29 DNA polymerase, a highly efficient enzyme able to produce strand displacement during the polymerisation process with high fidelity. Problems with DNA secondary structure were overcome and the
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4

Thornley, John K. "Methods of application of piezoelectric multilayer actuators to high-speed clutching, using displacement amplification." Thesis, Loughborough University, 1993. https://dspace.lboro.ac.uk/2134/10851.

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The suitability of electromagnetic actuating devices for application to machines with ever more demanding response time specifications is discussed, with the proposal that piezoelectric actuator technology can produce practical devices with faster response times than solenoids, for example. This thesis discusses and validates the proposition that the performance of piezoelectric ceramic actuators makes them viable devices for inclusion in high-speed machine applications, where rapid clutching using two-state actuation is required. Further, techniques are devised and explored for the design and
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5

Muharam, Firman Alamsyah. "Overcoming problems with limiting DNA samples in forensics and clinical diagnostics using multiple displacement amplification." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16207/.

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The availability of DNA samples that are of adequate quality and quantity is essential for any genetic analysis. The fields of forensic biology and clinical diagnostic pathology testing often suffer from limited samples that yield insufficient DNA material to allow extensive analysis. This study examined the utility of a recently introduced whole genome amplification method termed Multiple Displacement Amplification (MDA) for amplifying a variety of limited sample types that are commonly encountered in the fields of forensic biology and clinical diagnostics. The MDA reaction, which employs the
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Akinbami, Omolola Adetola. "Use of multiple displacement amplification based approaches for detection and analysis of environmentally significant and contaminating bacteria in fresh water." Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602407.

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One of the great challenges of microbial analysis in natural environments derives from the fact that a large proportion of microorganisms present is not culturable in standard conditions. To overcome this, various molecular approaches are widely used. Multiple Displacement Amplification (MDA) is especially useful as it can be applied in conjunction with other techniques to identify genes derived from individual microbial cells. The aim of this project was to study freshwater samples obtained from various environments in Ireland in order to identify dominant bacterial species and key genes asso
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7

Rönn, Ann-Charlotte. "Analysis of Nucleotide Variations in Non-human Primates." Doctoral thesis, Uppsala University, Molecular Medicine, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7904.

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<p>Many of our closest relatives, the primates, are endangered and could be extinct in a near future. To increase the knowledge of non-human primate genomes, and at the same time acquire information on our own genomic evolution, studies using high-throughput technologies are applied, which raises the demand for large amounts of high quality DNA.</p><p>In study I and II, we evaluated the multiple displacement amplification (MDA) technique, a whole genome amplification method, on a wide range of DNA sources, such as blood, hair and semen, by comparing MDA products to genomic DNA as templates for
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8

Brito, Luciana Carla Neves. "Análise microbiológica de infecções endodônticas utilizando a associação das técnicas do Multiple Displacement Amplification (MDA) e da Hibridização DNA-DNA (Checkerboard)." Universidade Federal de Minas Gerais, 2007. http://hdl.handle.net/1843/ZMRO-77LM2N.

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Multiple Displacement Amplification (MDA) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular analysis. The purpose of this investigation was to combine MDA and checkerboard DNA-DNA hybridization to examine the microbiota of endodontic infections. 66 samples were collected from teeth with endodontic infections. Non-amplified and MDA amplified samples were analyzed by checkerboard DNA-DNA hybridization for levels and proportions of 77 bacterial taxa. Counts, % DNA probe counts and % of teeth colonized for each species in amplif
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9

Halilovic, Amina. "SÄKERSTÄLLNING AV SÄLLSYNTA DNA-KONTROLLER MED HELGENOMAMPLIFIERING I KLINISKT SYFTE." Thesis, Malmö högskola, Fakulteten för hälsa och samhälle (HS), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-24387.

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Vid klinisk enbaspolymorfi (SNP) analys inkluderas DNA-kontroller med kända genotyper i varje analysomgång för att säkerställa riktigheten vad gäller analysresultatet. DNA-kontrollerna har en central roll för resultatens trovärdighet vid genotypningen. Vissa kontrollprover som används är av sällsynt genotyp och kan vara mycket svåra att få tag på. Detta arbete har utförts för att undersöka om det går att erhålla DNA-material från sällsynta genotyper med hjälp av helgenomamplifiering och på så sätt säkerställa en tillgång till dessa. I arbetet testades helgenomamplifiering med hjälp av två olik
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10

Yazgan, Ufuk. "A Methodology For Determination Of Performance Based Design Parameters." Master's thesis, METU, 2003. http://etd.lib.metu.edu.tr/upload/4/1120682/index.pdf.

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Establishment of relationships for predicting the lateral drift demands of near-fault ground motions is one of the major challenges in earthquake engineering. Excessive lateral drifts caused by earthquake ground motions are the major causes of structural damage observed in structures. In this study, some of the fundamental characteristics of near-fault ground motions are examined. Response characteristics of elastic frame structures to near-fault ground motions are investigated. An approximate method for estimating the elastic ground story and interstory drifts for regular frame type structure
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