Relevant bibliographies by topics / DNA antiguo

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'DNA antiguo'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'DNA antiguo.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "DNA antiguo"

1

Casas-Vargas, Andrea, Liza M. Romero, William Usaquén, Sara Zea, Margarita Silva, Ignacio Briceño, Alberto Gómez, and José Vicente Rodríguez. "Mitochondrial DNA diversity in prehispanic bone remains on the eastern Colombian Andes." Biomédica 37, no. 4 (December 1, 2017): 548. http://dx.doi.org/10.7705/biomedica.v37i4.3377.

Full text
Abstract:
Introducción. El ADN antiguo que se extrae de los restos óseos humanos permite analizar la composición genética de las poblaciones precolombinas y determinar las dinámicas poblacionales que dieron origen a la diversidad de las poblaciones contemporáneas.Objetivo. Determinar la diversidad genética y la relación con otras comunidades contemporáneas y antiguas de América, de los restos óseos asociados al Templo del Sol en Sogamoso, Colombia.Materiales y métodos. Se analizaron 13 individuos pertenecientes al periodo precolombino muisca (siglos IX-XVI d. C.), provenientes de los alrededores del Templo del Sol en Sogamoso, Boyacá, Andes orientales colombianos. Se amplificó el ADN mitocondrial (ADNmt) y se determinaron los polimorfismos de la longitud de los fragmentos de restricción (Restriction Fragment Length Polymorphism, RFLP) para los cuatro haplogrupos amerindios (A, B, C y D). Además, se amplificaron y analizaron los marcadores autosómicos, incluida la amelogenina, y los marcadores de los polimorfismos de repeticiones cortas en tándem (Short Tandem Repeat, STR) del cromosoma Y.Resultados. El haplogrupo A fue el linaje mitocondrial más frecuente en esta población, seguido de los haplogrupos B y C; no se detectó el haplogrupo D. Los análisis de variación genética indicaron una diversidad semejante a la de las poblaciones pertenecientes a la familia lingüística chibcha, contemporánea en Colombia y Centroamérica. Se logró hacer la determinación molecular del sexo de los individuos estudiados y compararla con los datos osteológicos. Con una sola excepción, los datos bioantropológicos y moleculares concordaron.Conclusiones. Estos resultados aportan nuevos elementos a la hipótesis del origen centroamericano de los grupos chibchas del altiplano cundiboyacense con base en marcadores genéticos, y permitieron establecer el sexo y las relaciones de parentesco.
APA, Harvard, Vancouver, ISO, and other styles
2

Sun, Jing, Dingfeng Li, Yanling Hao, Yuwei Zhang, Wenling Fan, Jingjing Fu, Yunzhang Hu, Yong Liu, and Yiming Shao. "Posttranscriptional Regulatory Elements Enhance Antigen Expression and DNA Vaccine Efficacy." DNA and Cell Biology 28, no. 5 (May 2009): 233–40. http://dx.doi.org/10.1089/dna.2009.0862.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Liu, Yang, Zhimin He, Dapeng Feng, Guodong Shi, Rui Gao, Xiaodong Wu, Weiguo Song, and Wen Yuan. "Cytotoxic T-lymphocyte Antigen-4 Polymorphisms and Susceptibility to Osteosarcoma." DNA and Cell Biology 30, no. 12 (December 2011): 1051–55. http://dx.doi.org/10.1089/dna.2011.1269.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

MARESH, GRACE A., JOHN S. MARKEN, MICHAEL NEUBAUER, ALEJANDRO ARUFFO, INGEGERD HELLSTRÖM, KARL ERIK HELLSTRÖM, and HANS MARQUARDT. "Cloning and Expression of the Gene for the Melanoma-Associated ME20 Antigen." DNA and Cell Biology 13, no. 2 (February 1994): 87–95. http://dx.doi.org/10.1089/dna.1994.13.87.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Klemen, Nicholas, Raul Vizcardo, Linda Tran, and Nicholas P. Restifo. "Precocious Differentiation of Somatic and Pluripotent Stem Cells Bearing Pre-Arranged TCR." Blood 126, no. 23 (December 3, 2015): 847. http://dx.doi.org/10.1182/blood.v126.23.847.847.

Full text
Abstract:
Abstract Adoptive cell transfer (ACT) of mutation-specific T lymphocytes may represent the future of immunotherapy for cancer. Current strategies utilize mature T cells isolated from tumors, but this approach may be hampered by limited persistence, advanced differentiation status, and exhaustion of transferred cells. Cellular rejuvenation is needed, and this could be accomplished through induced pluripotent stem cell (iPS) technology. IPS cells can be generated from various types of somatic cells using the four Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc), and we have shown that iPS generated from T cells (T-iPS) retain their original TCR specificity upon re-differentiation into T cell lineage. With existing technology, tumor antigen-specific progenitor cells can be derived from T-iPS. These cells could have superior potential to engraft, proliferate, and persist for long periods of time. To explore this concept, we have generated a murine iPS cell line from our Pmel transgenic mouse model, in which T cells bear a pre-rearranged TCR specific for the melanoma antigen GP100. Differentiation in vitro employs the OP9-DL1 co-culture system. We characterize the DN1-DN4 stages by flow cytometry of T cell lineage differentiation markers, such as c-kit, CD3, CD4, CD8, CD25, TCRvb, and TCRvb13 (present in the Pmel transgene). To study somatic stem cell differentiation, we used lineage negative bone marrow from femurs of wild type C57BL/6 (WT) and Pmel TCR transgenic (Pmel) mice. After 10 days of culture, cells were harvested and analyzed. Frequencies of cells in the DN1-DN4 stages for WT were 2%, 41%, 55%, and 2%; for Pmel they were 5%, 31%, 39%, and 25%. Expression of CD3 in WT cells in the DN1/DN2 stage was 1%, and for the DN3/DN4 stage was 2%. In contrast, CD3 expression in Pmel cells was 85% for cells in DN1/DN2 and 95% for cells in DN3/DN4. To study pluripotent stem cell differentiation, we used WT embryonic stem cells (ES) and Pmel iPS. After 21 days of culture, cells were harvested and analyzed. The double positive compartment was 9% in WT ES and 30% in Pmel iPS; the double negative compartment was 60% and 37%. Frequencies of cells in DN1-DN4 stages for WT ES were 4%, 6%, 45%, and 45%; for Pmel they were 1%, 7%, 69%, and 23%. Expression of CD3 in WT cells in the DN1/DN2 stage was 1%, and for the DN3/DN4 stage was 13%. In contrast, CD3 expression in Pmel cells was 76% for cells in DN1/DN2 and 91% for cells in DN3/DN4. These data show for the first time that in vitro differentiation of pluripotent stem-derived progenitor cells bearing a pre-rearranged TCR will undergo precocious expression of the CD3 molecule and enhanced progression to DP stage as compared to WT controls. The pattern is consistent with that previously seen during in vitro differentiation of somatic stem cells. It appears that the presence of a pre-rearranged TCR is driving precocious development of T cells in vitro, regardless of whether the source of progenitors is from somatic (bone marrow) or pluripotent (ES/iPS) stem cells. Our ultimate goal is to intricately characterize the nature of iPS-derived T cells, and study their antitumor efficiency in vitro and in vivo, in order to evaluate their suitability for clinical use. Disclosures No relevant conflicts of interest to declare.
APA, Harvard, Vancouver, ISO, and other styles
6

Lang, Cuicui, Lei Chen, and Senlin Li. "Cytotoxic T-Lymphocyte Antigen-4 +49G/A Polymorphism and Susceptibility to Pancreatic Cancer." DNA and Cell Biology 31, no. 5 (May 2012): 683–87. http://dx.doi.org/10.1089/dna.2011.1417.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

MURALIKRISHNA, T., ZAREENA BEGUM, Ch V. B. SWAMY, and ASHOK KHAR. "Molecular Cloning and Characterization of a Tumor Rejection Antigen from Rat Histiocytoma, AK-5." DNA and Cell Biology 17, no. 7 (July 1998): 603–12. http://dx.doi.org/10.1089/dna.1998.17.603.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Ou, JianFeng, Kang Li, Hui Ren, Hai Bai, Dan Zeng, and ChongJie Zhang. "Association and Haplotype Analysis of Prostate Stem Cell Antigen with Gastric Cancer in Tibetans." DNA and Cell Biology 29, no. 6 (June 2010): 319–23. http://dx.doi.org/10.1089/dna.2009.0960.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Hassen, Elham, Randa Ghedira, Nahla Ghandri, Karim Farhat, Sallouha Gabbouj, Noureddine Bouaouina, Hamdi Abdelaziz, Abdelatif Nouri, and Lotfi Chouchane. "Lack of Association Between Human Leukocyte Antigen-E Alleles and Nasopharyngeal Carcinoma in Tunisians." DNA and Cell Biology 30, no. 8 (August 2011): 603–9. http://dx.doi.org/10.1089/dna.2010.1140.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

SAWADA, RITSUKO, KENSAKU OHASHI, HIROYUKI ANAGUCHI, HITOAKI OKAZAKI, MASAKAZU HATTORI, NAGAHIRO MINATO, and MASANOBU NARUTO. "Isolation and Expression of the Full-Length cDNA Encoding CD59 Antigen of Human Lymphocytes." DNA and Cell Biology 9, no. 3 (April 1990): 213–20. http://dx.doi.org/10.1089/dna.1990.9.213.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "DNA antiguo"

1

Solórzano, Navarro Eduvigis. "De la Mesoamérica Prehispánica a la Colonial: La huella del DNA antiguo." Doctoral thesis, Universitat Autònoma de Barcelona, 2006. http://hdl.handle.net/10803/3682.

Full text
Abstract:
Este trabajo es una contribución al estudio de la diversidad genética en las poblaciones americanas antiguas. Se ha analizado el DNA procedente de restos humanos esqueléticos de yacimientos mesoamericanos de tres épocas distintas, con dataciones que se ubican desde la época azteca prehispánica (post-clásico tardío) a la colonia del Virreinato de la Nueva España, con la finalidad de inferir, desde la visión de los linajes maternos, la dinámica de las poblaciones del Valle Central de México y el posible aporte genético del contingente español y africano que llegó a tierras americanas, específicamente a México, desde finales del siglo XVI.
Se estudiaron los marcadores del DNA mitocondrial (mtDNA) a partir de restricción enzimática de fragmentos en la región codificante y de la secuenciación de un segmento de la región hipervariable I. Los distintos análisis se realizaron observando un estricto control de los criterios de autenticidad en relación a las condiciones de laboratorio, el uso de controles, la caracterización de los investigadores, diversidad genética, sentido filogenético y total correspondencia entre los marcadores mitocondriales, concordancia que es reconocida como un criterio de autenticidad cuando se analiza el mtDNA proveniente de restos antiguos.
De los ciento dos individuos estudiados, ochenta y siete fueron clasificados entre los cuatro principales haplogrupos descritos para nativos americanos (A, B, C y D) y tres no segregaron para ninguno de estos haplogrupos, ni siquiera para el quinto y menos frecuente linaje americano, el haplogrupo X; a la luz de los datos de que se dispone hasta el momento es probable de que se trate de individuos cuyo linaje maternal pertenezca a alguno de los haplogrupos africanos (L1, L2 y L3), siendo la primera evidencia genética del aporte africano en época colonial. En los doce individuos restantes no se lograron amplificaciones positivas para más de un sitio de restricción, por lo cual fueron excluidos de la investigación.
Los análisis de comparación entre las tres series antiguas permiten deducir una continuidad entre los linajes mitocondriales anteriores al contacto europeo y los linajes de la época colonial, no observándose diferencias significativas entre ellas. Sin embargo, la presencia de secuencias únicas en la serie de contacto permite hipotetizar un colapso poblacional en algunos núcleos indígenas. Los resultados obtenidos tanto a nivel de haplogrupos como de secuencias también han sido comparados con datos de poblaciones actuales y antiguas de América y Asia obtenidos de la literatura; y de esta manera, situar en el contexto poblacional americano las muestras antiguas del Valle Central mexicano.
Los procedimientos de reconstrucción filogenética nos permiten deducir que las series antiguas del Valle Central de México tienen un vínculo por vía matrilineal con el resto de las poblaciones americanas, y especialmente con la población mexicana contemporánea de referencia. Además, está virtualmente ausente el aporte europeo en las muestras analizadas, debido posiblemente, a que el proceso de mestizaje que se produjo durante los siglos XVI y XIX fue de tipo unidireccional, hombre europeo-mujer indígena y el mtDNA sólo nos permite el análisis del aporte genético materno.
This paper is a contribution to the genetic diversity study in ancient American populations. DNA from Mesoamerican human skeletal remains from three different periods, which cover from the pre-Hispanic Aztec epoch (late post-classical) to the Viceroyalty of New Spain at the colonial period were analyzed, with the purpose to infer, with the information that the maternal lineages can provide us, Mexico's Central Valley population dynamics; and the possible genetic contribution of the Spanish and African contingents that arrived to the Americas, specifically to Mexico, since the last period of the XVIth century.
Mitochondrial DNA (mtDNA) markers have been studied by both specific restriction enzyme analysis in the coding region and by sequencing of the hypervariable region I segment. The analyses were carried out with a strict control of the authenticity criteria, focusing on: laboratory conditions, use of blank controls, mtDNA characterization of laboratory researchers, genetic diversity, phylogenetic sense and total correspondence among mitochondrial markers, which is recognized as an authenticity criterium where mtDNA analysis from ancient remains is concerned.
Of the hundred two individuals studied, eighty-seven were classified among the four major founding mtDNA haplogroups described for American natives (A, B, C, and D), three individuals didn't segregate for any of these haplogroups, not even for the fifth and less frequent American founding lineage, the haplogroup X; and it is probable that their maternal lineage belong to one of the African haplogroups (L1, L2 or L3), being the first genetic evidence of the African contribution in the colonial epoch. Finally, in twelve individuals positive amplifications were achieved in no more than one restriction site, reason by which they were excluded of the investigation.
The analysis comparison among the three ancient series showed that there is continuity between mitochondrial lineages previous to the European contact and colonial lineages, and that there is not a significant difference among them. Nevertheless, the presence of exclusive lineages in contact series allows us to hypothesize a population collapse in some native groups. The results obtained using both methods of the mtDNA analysis have also been compared with ancient and current populations data from America and Asia available in the literature; and in this way, we have been able to place in the American context the Mexico's Central Valley samples.
Phylogenetic reconstruction procedures permit to deduce that Mexico's Central Valley ancient series have a maternal link with the remaining American populations, and especially with the Mexican current population of reference. In addition the European contribution in the samples analyzed is virtually absent possibly owing to that the mestizaje process that was produced during the XVIth and XIXth centuries was of one-directional: European man - Native woman and mtDNA only permits maternal genetic analysis.
APA, Harvard, Vancouver, ISO, and other styles
2

Montiel, Duarte Rafael. "Estudio diacrónico de la variabilidad del DNA mitocondrial en población catalana." Doctoral thesis, Universitat Autònoma de Barcelona, 2001. http://hdl.handle.net/10803/3641.

Full text
Abstract:
El DNA mitocondrial (mtDNA) es útil para estudios de genética de poblaciones humanas debido a que su genoma está completamente caracterizado, es de herencia materna y tiene una tasa de evolución relativamente rápida. Las técnicas de DNA antiguo, que empezaron a desarrollarse en 1984, han añadido una variable temporal a este tipo de estudios a pesar de que existe cierta desconfianza debido al riesgo de contaminación de las muestras con DNA moderno. Por otra parte, el estudio de la población Catalana resulta interesante debido a que el análisis de componentes principales de marcadores clásicos mostró su diferenciación respecto a otras poblaciones ibéricas; diferenciación que fue corroborada por estudios de comparación de secuencias de mtDNA.
Bajo estas consideraciones fue planteado como objetivo principal valorar las posibilidades que tienen los estudios de DNA antiguo a nivel poblacional, en la resolución de problemas como la estimación de la tasa de evolución y el origen del acervo genético mitocondrial europeo y el desarrollo de métodos fiables para la detección de la contaminación con DNA exógeno. Con este fin se analizó la población Catalana de dos épocas diferenciadas.
Para el estudio de la población antigua se analizaron 52 individuos de la necrópolis de la Plaça Vella de Terrassa, Barcelona (s. XV-XVI) y se obtuvo mtDNA de 24 de ellos. En este análisis también se incluyeron diversos individuos de distintos yacimientos a manera de control metodológico. Para el análisis de la población contemporánea se caracterizó el mtDNA de 90 individuos de población residente de las ciudades de Terrassa y Barcelona y estudiantes de la Universidad Autónoma de Barcelona.
En los 24 individuos de la Plaça Vella se encontraron 12 secuencias diferentes con un índice de diversidad que cae dentro del rango obtenido para otras poblaciones europeas. En estos individuos están representados 7 de los 9 haplogrupos europeos y la distribución que presentan también es similar a la que presentan la mayoría de poblaciones europeas. Estas observaciones junto con distintos criterios discutidos en esta tesis avalan la autenticidad de los resultados obtenidos.
Con estos datos se realizó un análisis filogenético incluyendo información previamente publicada de 11 series poblacionales principalmente europeas. El análisis mostró una cercana relación entre las poblaciones antigua y actual de Cataluña y evidenció también la cercanía entre estas poblaciones y otras poblaciones mediterráneas en cuanto a su mtDNA. En cambio, la relación de la población Catalana con otras poblaciones ibéricas (Galicia y País Vasco) no resultó tan estrecha.
Paralelamente a los estudios de las poblaciones antigua y actual, se llevó a cabo una investigación sobre las substancias que presentan los extractos de DNA antiguo que tienen un poder inhibitorio en las reacciones enzimáticas. El análisis comparativo apoya la hipótesis de que estas substancias sean alguna fracción de los ácidos húmicos, descartando las porfirinas, el daño del DNA y los iones libres de hierro. La hipótesis de que sean productos Maillard no fue rechazada pero no se obtuvo evidencia en favor de ella. Además, se encontró un método sencillo que disminuye los efectos negativos que presentan estas substancias durante la Reacción en Cadena de la Polimerasa (PCR).
Mitochondrial DNA (mtDNA) is useful in population genetics studies because its genome is fully characterised, it is maternally inherited and it shows a fast evolution rate. Ancient DNA techniques, beginning in1984, have added a temporal variable to this kind of studies although there is some controversy around this kind of analysis, due to contamination problems with modern DNA. The study of the Catalonian population is interesting since previous reports have shown some differentiation of this population from other Iberian populations, by means of principal components analysis for classic markers. This differentiation has been corroborated in some studies by comparing mtDNA sequences.
Under these considerations, the main objective of the present work was to assess the ability of ancient DNA studies - at population level - to solve problems such as the evolution rate estimation, the origin of the mitochondrial gene pool in Europe, and the development of reliable methods to detect exogenous DNA contamination. For this purpose, Catalonian populations from two different periods were analysed.
For the ancient population, 52 individuals from the necropolis of PlaçaVella, Terrassa, Barcelona (XV-XVI centuries) were analysed. Mitochondrial DNA was recovered from 24 individuals. As a methodological control, several individuals from different sites were included in this study. For the contemporary population, the mtDNA from 90 contemporary individuals inhabitants of Terrassa and Barcelona cities and students from the Universitat Autònoma de Barcelona was analysed.
There were 12 different sequences in the 24 individuals from the PlaçaVella, which showed an index of diversity fitting in the range observed for other European populations. These 24 individuals presented 7 out of the 9 European haplogroups and their distribution looked like most of the European populations. These observations, along with another criteria discussed in this work, support the authenticity of the results.
The data obtained from the ancient population as well as from the contemporary population, were used in a phylogenetic analysis, including previously published information from 11 population series, mainly European. This analysis showed a close relationship between the ancient and contemporary populations from Catalonia, as well as between these populations and other Mediterranean populations, regarding its mtDNA. On the other hand, the relationship between Catalonian population and other Iberian populations (Galicia and Basque Country) was not so close.
Besides the studies on ancient and contemporary populations, a research on the inhibitory substances usually found in ancient DNA extracts was carried out. The comparative analysis supports the hypothesis that these substances are a humic acid fraction, discarding another substances as the putative inhibitors. The possibility that these substances are Maillard reaction products was not rejected; however, there was no evidence supporting this possibility. In this work, a novel and simple method to overcome the PCR inhibition produced by these substances was uncovered.
APA, Harvard, Vancouver, ISO, and other styles
3

Galimany, Skupham Jacqueline Lorna. "Patrones de parentesco y residencia mediante DNA antiguo en el uso mortuorio de la cueva Estero Sur, Archipiélago de los Chonos." Tesis, Universidad de Chile, 2015. http://repositorio.uchile.cl/handle/2250/136512.

Full text
Abstract:
Antropóloga Física
El grupo canoero más septentrional del archipiélago patagónico occidental y el primero en extinguirse es conocido etnohistóricamente como Chono. Lo que sabemos de su organización social se basa en observaciones no sistemáticas de la etnia post-contacto y las características y alteración de los contextos mortuorios de cuevas y aleros rocosos solo permiten un mero vistazo a lo que fue este grupo. La reciente validación de la unidad genética de los antiguos habitantes del Archipiélago de los Chonos nos permite plantear nuevas interrogantes. El sitio mortuorio cueva Estero Sur constituye el osario más antiguo (~2000 AP) y numeroso hallado en la zona. La mínima divergencia de sus fechados lo define como un contexto ideal en la búsqueda de patrones de parentesco y residencia. Para ello, se caracterizó los haplotipos de DNA mitocondrial y cromosoma Y de los individuos de la cueva Estero Sur y una muestra control de fechados cercanos hallada en contextos mortuorios de cueva del Archipiélago de los Chonos. La frecuencia, diversidad y distancia genética de los linajes presentes en los individuos de ambos conjuntos indican un patrón probablemente aleatorio de uso mortuorio del sitio, descartando su uso exclusivo por parte de una familia nuclear, un matrilinaje o un patrilinaje. Este patrón concuerda con una depositación mortuoria dispersa, consecuencia de una alta y amplia movilidad
APA, Harvard, Vancouver, ISO, and other styles
4

Fehren-Schmitz, Lars, Bastien Llamas, Elsa Tomasto, and Wolfgang Haak. "Ancient DNA and the Early Population History of Western South America: What Have We Learned So Far and Where Do We Go From Here." Pontificia Universidad Católica del Perú, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/113534.

Full text
Abstract:
Even though the analysis of DNA from archaeological bone comes with some major limitations, it constitutes the most directmeans of investigating prehistoric population dynamics. The interdisciplinary contextualization of genetic data with the archaeological and palaeoecological record helps to reconstruct past population histories and the demography of ancient populations. For South America, palaeogenetic studies have become increasingly important. Here we review the existing ancient DNA data from pre-Columbian individuals to assess their potential to contribute to our understanding of early South American population history. The spatial and temporal distribution of ancient South American populations analysed to date is very uneven and the data resolution of the analysed genetic markers is low. Nevertheless, the data suggest that there were population dynamic processes accompanying cultural development in Western South America. With the new methodologies and better sampling strategies employed in current paleogenetic projects and more effective interdisciplinary cooperations it will be soon possible to achieve a better understanding of the peopling of the continent and the succeeding population history.
Aún cuando el análisis de ADN de huesos arqueológicos tiene algunas grandes limitaciones, constituye la manera más directa de investigar eventos prehistóricos de dinámica poblacional. La contextualización interdisciplinaria de los datos genéticos con los registros arqueológico y paleoecológico permite reconstruir las historias poblacionales pasadas y la demografía de sociedades antiguas. Por otro lado, el número de estudios paleogenéticos en Sudamérica se está incrementando. En este artículo revisamos los datos de ADN antiguo de individuos prehispánicos que existen en la actualidad con la finalidad de evaluar su potencial para contribuir a nuestro entendimiento de la historia temprana del poblamiento de Sudamérica. La distribución espacial y temporal de las poblaciones sudamericanas antiguas muestreadas a la fecha es muy irregular y la resolución de los marcadores genéticos analizados esbaja. Sin embargo, los datos sugieren que existieron procesos de dinámica poblacional que acompañaron el desarrollo cultural de la parte oeste de Sudamérica. Con las nuevas metodologías y mejores estrategias de muestreo que se emplean hoy en día en los proyectos de paleogenética, y con una cooperación interdisciplinaria más efectiva, pronto será posible lograr un mejor entendimiento del poblamiento del continente, así como de los hechos sucesivos de su historia poblacional.
APA, Harvard, Vancouver, ISO, and other styles
5

Fehren-Schmitz, Lars. "Pre-Columbian Population Dynamics and Cultural Development in South Coast Perú as Revealed by Analysis of Ancient DNA." Pontificia Universidad Católica del Perú, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/113298.

Full text
Abstract:
In this paper I report on a study whose principal aim is to understand the development and decline of the southern Peruvian Nasca culture in the upper Río Grande de Nasca drainage, and its cultural and biological affinities to the preceding Paracas culture. Ancient DNA analyses were conducted on over 300 pre-Columbian individuals from various cemeteries in southern Perú, from periods ranging from the Formative Period to the Middle Horizon. Our results show that the Nasca populations are close related to those of the preceding Paracas culture, and combined with archaeological data, suggest that the Nasca culture was autochthonous to the Río Grande drainage. Furthermore, one can observe how changes in socioeconomic complexity influence the genetic diversity. The pre-Columbian coastal populations of southern Perú differ significantly from both ancient highland and all present-day Peruvian populations. The genetic differentiation between the main cultural areas of western South America seems to fade with the Middle Horizon.
Se presenta aquí un estudio cuyo objetivo principal es la comprensión del desarrollo y decadencia de la cultura Nasca en la parte alta de la cuenca del Río Grande de Nasca, así como sus afinidades biológicas y culturales con su antecesora, la cultura Paracas. Se realizaron análisis de ADN antiguo en más de 300 individuos procedentes de varios cementerios prehispánicos del sur del Perú correspondientes a un lapso que se inicia en el Período Formativo y alcanza el Horizonte Medio. Los resultados muestran que las poblaciones nasca son cercanas a las de su cultura precedente. Esta información, combinada con los datos arqueológicos, sugiere que la cultura Nasca se desarrolló, de manera autóctona, en la cuenca del Río Grande. Más aún, se puede observar que los cambios socioeconómicos de este período influyeron en la diversidad genética. Las poblaciones prehispánicas costeñas del sur del Perú difieren, significativamente, de las antiguas poblaciones de la sierra y de las poblaciones peruanas actuales. La diferenciación genética entre las principales áreas culturales de la parte oeste de Sudamérica parece desaparecer en el Horizonte Medio.
APA, Harvard, Vancouver, ISO, and other styles
6

Ferrando-Bernal, Manuel 1990. "Analysis of co-ancestry links in modern and ancient human populations." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2021. http://hdl.handle.net/10803/672475.

Full text
Abstract:
The aim of this thesis is to apply Identity by Descent (IBD) methodology to identify ancestry connections among individuals from genetically similar populations. Recombination events diminish the likelihood to detect IBDs. As most of the aDNA samples date from 2,000 years ago or more, this methodology has rarely been applied to these studies. In this thesis we detect IBD among modern individuals from similar Bantu populations and among modern Europeans with an historical individual (700 years ago) from the Iberian peninsula, which was sequenced to a high coverage. Our results show that IBDs can be used to detect the genomic structure in genetically close populations. For example, they can be used to show high degrees of endogamy caused by isolation or to identify ancestral connections among individuals belonging to different populations that otherwise would be difficult to see with other more commonly used methods.
APA, Harvard, Vancouver, ISO, and other styles
7

Sampietro, Bergua Mª Lourdes. "Genetic Analysis of the prehistoic peopling of Western Europe: Ancient DNA the role of contamination." Doctoral thesis, Universitat Pompeu Fabra, 2007. http://hdl.handle.net/10803/79128.

Full text
Abstract:
In this thesis we have addressed three different although related topics. First, we studied the post-mortem mutation damage rate of contaminated DNA sequences in ancient human remains focusing on the development of strategies to avoid pre-laboratory derived contaminations. We proposed a guideline to control them consisting in typing every single person involved on the manipulation of the remains, especially when they have not been excavated and washed under controlled conditions. Second, we successfully develop a non-invasive technique to sequence ancient remains but preserving it from the destruction. And third, we sequenced ancient human remains from different evolutionary times (from Paleolithic to post-Neolithic) to make inferences about the peopling of Western Europe focusing mainly in the Iberia peninsula. We found that there is a long term genetic continuity at least since the Neolithic. The only clear genetic discontinuity found is that involving two different human species, H. sapiens and H. neanderthalensis.
En la presente tesis hemos tratado tres temas diferentes aunque muy relacionados. Primero, hemos estudiado la tasa de mutación post-mortem de secuencias de ADN contaminante en restos humanos antiguos centrándonos en el desarrollo de estrategias para evitar que las muestras se contaminen antes de llegar al laboratorio. Proponemos una guía que consiste en el tipado genético de cada persona implicada en la manipulación de los restos, especialmente cuando estos han sido excavados y lavados bajo condiciones no controladas. Segundo, hemos desarrollado una técnica no invasiva para secuenciar DNA de restos humanos antiguos pero sin destruirlos. Y por ultimo, hemos secuenciado restos humanos antiguos pertenecientes a diferentes periodos evolutivos (desde el Paleolitico hasta el post-Neolitico) que nos han permitido hacer inferencias sobre el poblamiento Europeo centrándonos básicamente en la Península Ibérica. Hemos encontrado que ha habido una continuidad genética desde el Neolítico. La única clara discontinuidad genética encontrada es entre dos especies distintas: H. Sapiens y H.neanderthalensis.
APA, Harvard, Vancouver, ISO, and other styles
8

Song, Keli 1955. "DNA-based vaccination against carcinoembryonic antigen." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36837.

Full text
Abstract:
DNA vaccination is based on in vivo delivery of plasmids encoding an antigen, leading to antigen synthesis and specific immunity. This technology is still in its infancy but has advantages over conventional vaccination, such as the stimulation of all arms of the immune response, i.e., humoral immunity, T-helper (17h) cells and cytotoxic T lymphocytes (CTLs). We studied DNA vaccination against human carcinoembryonic antigen (CEA) in mice. Our hypothesis was that codelivery of cytokine- and CEA-encoding plasmids could regulate responses, and allow polarization of Th responses to either type 1 (Th1) or type 2 (Th2). We found that intramuscular injection of the CEA plasmid alone induced antibodies, Th1 cells and CTLs reactive to CEA. These mice had increased immunity against transplanted syngeneic CEA+ stably transfected tumor cell lines, but always developed lethal tumors. Coinjection of the CEA plasmid with a vector encoding either IL-12 or interferon gamma (IFNgamma) markedly enhanced IgG2a production (IFNgamma-dependent), IFNgamma secretion by spleen cells (a Th1 cytokine) and CTL-mediated tumor cell lysis, in a CEA-specific way. Moreover, resistance to a tumor challenge was greatly improved, such that up to 80% of mice survived tumor free. In contrast, coinjection of CEA and IL-4 genes increased CEA-specific IgG1 levels (IL-4-dependent) and IL-4 secretion by lymphocytes (a Th2 cytokine), but decreased both CTL activity and tumor resistance. Thus, we could readily enhance or polarize immunity. The IL-12 cDNA had the strongest adjuvant effect, which was only observed when it was injected at the same site as the CEA gene. To analyze effector components, we studied IL-12-plasmid-enhanced DNA vaccination in gene knockout mice, lacking either CD3, CD4, CD8. IFNgamma, perforin or Fas ligand (FasL). Only mice expressing all of CD3, CD4, IFNgamma, CD8 and perforin, and inoculated with both the CEA and IL-12 genes, could fully resist a tumor challenge. The Fas/FasL lytic
APA, Harvard, Vancouver, ISO, and other styles
9

López, de Rioja Víctor. "Population range expansions, with mathematical applications to interacting systems and ancient human genetics." Doctoral thesis, Universitat de Girona, 2019. http://hdl.handle.net/10803/667171.

Full text
Abstract:
The thesis studies from an analytical and computational perspective, and by using reaction-diffusion equations, the spatiotemporal evolution of different populations. First, the dynamics of the T7 bacteriophage infecting the E. coli bacteria is studied. By adding the delayed time in diffusion and reaction terms, as well as new mathematical terms biologically sound, we can achieve results that accurately match the experimental propagation speeds. Secondly, different mathematical models are proposed to correctly understand the expansion of VSV in Glioblastoma. The only model capable of this explanation is the system which understands the delay time for the processes of diffusion and reaction. Finally, the Neolithic transition through Europe is explained by studying ancient genetic DNA samples alongside mathematical simulations. Focusing on haplogroup K, the model is built by analyzing the two Neolithic diffusion mechanisms: demic and cultural. The simulations show that the transition is basically demic, with only 2% of the Neolithic farmers interacting culturally
Aquesta tesi estudia des d’un punt de analític i computacional, gràcies a les equacions de reacció-difusió, l’evolució espaciotemporal de diferents poblacions que interactuen entre elles. El primer article estudia la dinàmica del bacteriòfag T7 infectant el bacteri E. coli. Gràcies a la incorporació del temps de retard en els termes de difusió i reacció, així com de nous termes matemàtics amb sentit biològic, aconseguim uns resultats que s’ajusten millor a les velocitats de propagació. El segon article aplica diferents models matemàtics per entendre millor l’expansió del VSV en Glioblastomes. L'únic model capaç d'explicar de manera correcte el sistema té en compte el temps de retard per als processos de difusió i reacció. L’últim article explica la transició del Neolític a través d’Europa utilitzant mostres genètiques antigues i simulacions matemàtiques. Centrant-nos en l’haplogrup K, el model es construeix tenint en compte els dos mecanismes de difusió neolítica: dèmica i cultural. Les simulacions mostren que la transició és bàsicament dèmica, on només el 2% dels neolítics interaccionen culturalment
APA, Harvard, Vancouver, ISO, and other styles
10

Ola, Ayodele Oluronke. "A functional analysis of proliferating cell nuclear antigen (PCNA)." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391441.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "DNA antiguo"

1

Lee, Hoyun. Proliferating cell nuclear antigen (PCNA). Trivandrum, Kerala, India: Research Signpost, 2006.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Pollwein, Peter. Spezifische Bindungsstellen von SV40 T-Antigen im zellulären Mausgenom. Konstanz: Hartung-Gorre, 1987.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Beadchip molecular immunohematology: Toward routine donor and patient antigen profiling by DNA analysis. New York: Springer, 2011.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

O Convento da Graça: Antigo mosteiro de São Francisco de Loulé : monografia histórico-artística. Lisboa: Edições Colibri, 2008.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Bastianini, Guido, Nikolaos Gonis, and Simona Russo, eds. Charisterion per Revel A. Coles. Florence: Firenze University Press, 2016. http://dx.doi.org/10.36253/978-88-6655-827-9.

Full text
Abstract:
Questo volume, dedicato a Revel Coles (che per tanti anni ha lavorato alla collezione degli Oxyrhynchus Papyri di Oxford), contiene l’edizione di trenta testi di provenienza egiziana, appartenenti a varie collezioni italiane e straniere, curati da papirologi che con Revel Coles hanno collaborato in Inghilterra e in Italia. I testi sono quasi tutti in greco (uno è in latino e uno in copto), ed offrono una campionatura significativa della cultura e della società dell’Egitto antico, dal II secolo a.C. all’VIII d.C.: vi figurano frammenti di opere dei principali autori della letteratura greca (come Omero, Esiodo, Euripide), e anche testi di magia applicata e di contenuto liturgico; vi sono compresi inoltre documenti della quotidianità, come petizioni e istanze di vario tipo, un verbale di processo riguardante un funzionario corrotto, ricevute di tasse, rendiconti della pubblica amministrazione e di imprese private, un contratto di permuta di immobili. Gli studiosi del mondo antico greco e romano, nei suoi vari aspetti, troveranno in questo volume numerosi elementi di interesse.
APA, Harvard, Vancouver, ISO, and other styles
6

Sitchin, Zecharia. Hubo gigantes en la tierra: Dioses, semidioses y ancestros humanos : la evidencia de un ADN extraterrestre. Barcelona: Ediciones Obelisco, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Aristotle in outline. Indianapolis: Hackett, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Casanova, Angelo Alfredo, ed. Menandro e l’evoluzione della commedia greca. Florence: Firenze University Press, 2015. http://dx.doi.org/10.36253/978-88-6655-668-8.

Full text
Abstract:
Il volume contiene diciotto saggi originali, scritti – su invito – da studiosi di fama internazionale, provenienti da varie università italiane o straniere. Si affrontano le maggiori questioni che i recenti ritrovamenti papiracei hanno sollevato (o riproposto) sul testo e l’intepretazione di diverse commedie di Menandro, delineando un quadro autorevole e pienamente aggiornato delle nostre conoscenze in proposito. Si offre tra l’altro la primizia dell’edizione (con fotografia) di un nuovo frammento, finora inedito, del famoso (e discusso) papiro Michigan. Tutte le relazioni presentano aspetti notevoli di novità editoriale, con commenti di tipo filologico, linguistico e letterario, rilevanti per il dibattito culturale sulla commedia di Menandro, e quindi per la storia del teatro antico e della sua evoluzione, nonché per lo studio delle influenze esercitate dalla Commedia Nuova sulla tradizione posteriore, latina e italiana.
APA, Harvard, Vancouver, ISO, and other styles
9

Os últimos faustos do antigo regime: Narração do solemne baptismo do Sereníssimo Senhor D. António Princípe da Beira : celebrado no Real Palácio de Quelus : no dia 4 de Abril do anno de 1795. Lisboa: Chaves Ferreira Publicações, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Dessì, Giuseppe, and Mario Pinna. Tre amici tra la Sardegna e Ferrara. Edited by Costanza Chimirri. Florence: Firenze University Press, 2013. http://dx.doi.org/10.36253/978-88-6655-478-3.

Full text
Abstract:
Una Sardegna riservata e lontana anima i testi di questo doppio carteggio, tra paesaggi arcaici e mitologie personali e letterarie nelle quali si inserisce ogni tanto la Ferrara degli anni giovanili degli autori, ricca di vita, di riviste («Primato» di Bottai, il «Corriere Padano» con la presenza di Bassani…), incontri serali nelle osterie o nelle camere in affitto, passeggiate lungo i Rampari, e l’uso di scherzosi soprannomi che sarebbe continuato oltre la giovinezza. Un mondo fatto di cose concrete, animato e vivificato da forti curiosità e passioni intellettuali, emerge dalle lettere, accuratamente trascritte e annotate da Costanza Chimirri, che ricostruiscono la vita e la storia di Giuseppe Dessí, Mario Pinna, Claudio Varese. La corrispondenza si apre con gli anni trascorsi a Ferrara – dopo Pisa momento cruciale per la loro formazione – e consente di ricostruire atmosfere ed ambienti, letture e lavoro, offrendo dall’interno un significativo spaccato dell’Italia del Novecento. Mai slegati tra loro, bensì uniti dal continuo richiamo alla triplice amicizia nel nome di Giuseppe Dessí, che è sempre presente, anche in assenza, nei discorsi degli altri, i carteggi hanno consentito anche di riportare alle luce testi inediti del più appartato del gruppo (Mario Pinna, accanito lettore di classici, ispanista, autore di poesie in dialetto logudorese e di brevi racconti ambientati in Sardegna), di rafforzare il ruolo da sempre ricoperto dal più ‘antico’ – per tutti maître-camarade – Claudio Varese; e di confermare ancora una volta quanto l’universo creativo di Dessí, profondamente segnato dalla componente biografica, abbia continuato a svilupparsi e alimentarsi sotto lo sguardo sapiente e affettuoso di amici fraterni, in uno scambio capace di dare vita a un vero e proprio immaginario collettivo.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "DNA antiguo"

1

Scheiblhofer, Sandra, Uwe Ritter, Josef Thalhamer, and Richard Weiss. "Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)." In Biolistic DNA Delivery, 401–11. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-110-3_29.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Smeenk, Ruud J. T. "DNA as antigen in SLE." In Manual of Biological Markers of Disease, 237–382. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-5444-4_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Smeenk, Ruud J. T. "DNA as antigen in SLE." In Manual of Biological Markers of Disease, 245–59. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-1670-1_17.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Heeg, K. "CpG DNA Co-Stimulates Antigen-Reactive T Cells." In Immunobiology of Bacterial CpG-DNA, 93–105. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59672-8_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Scheffner, M., R. Wessel, and H. Stahl. "SV40 T Antigen Catalyzed Duplex DNA Unwinding." In Transforming Proteins of DNA Tumor Viruses, 37–45. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74578-2_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Corr, Maripat, and Helen Tighe. "Plasmid DNA vaccination: mechanism of antigen presentation." In Gene Vaccination: Theory and Practice, 9–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-46867-4_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Kirkman, Laura A., and Kirk W. Deitsch. "Recombination and Diversification of the Variant Antigen Encoding Genes in the Malaria Parasite Plasmodium falciparum." In Mobile DNA III, 437–49. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555819217.ch20.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Lazaro, Ana, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, and Carolyn Katovich Hurley. "Human Leukocyte Antigen (HLA) Typing by DNA Sequencing." In Methods in Molecular Biology, 161–95. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-493-7_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Sparwasser, T., and G. B. Lipford. "Consequences of Bacterial CpG DNA-Driven Activation of Antigen-Presenting Cells." In Immunobiology of Bacterial CpG-DNA, 59–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59672-8_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Chu, R. S., D. Askew, and C. V. Harding. "CpG DNA Switches on Th1 Immunity and Modulates Antigen-Presenting Cell Function." In Immunobiology of Bacterial CpG-DNA, 199–210. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59672-8_14.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "DNA antiguo"

1

Merlo, Manuele, Fabio Negretto, and Franco Maria Montevecchi. "The Effect of the Electrostatic Field on the Deflection of Microcantilever Biosensors." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95155.

Full text
Abstract:
Microcantilever biosensors offer the capability to detect specific molecular binding like complementary DNA fragment hybridization or specific antibody-antigen binding. The cantilever deflection, which can be optically detected, is caused by the adsorption of biological molecules like DNA fragments upon the microcantilever surface and the subsequent specific binding to the complementary species. The cantilever deflection is due to the surface stress induced by the free energy variation on the cantilever surface. Contributions to the free energy variation come from a number of interactions within the molecules, such as electrostatic interactions, biomolecule conformational entropy and internal energy variation, hydration forces. In the present work the effect of the electrostatic field within DNA biomolecules on the cantilever deflection is investigated. The electrostatic field within double strand DNA molecules is studied by means of a Finite Element (FE) analysis aimed at numerically solving the non linear Poisson Boltzmann equation (PBE) in the domain representing the biomolecule system. The electrostatic analysis has been coupled to a FE structural analysis in order to evaluate the influence of the electrostatic field on the cantilever deflection. The double strand DNA molecules are modelled as a periodic disposition of cylinders negatively charged at the surface. The hexagonal and square DNA molecule patterns were compared, and the Manning condensation hypothesis was discussed. The results are shown for different operating conditions and compared with experimental data from literature.
APA, Harvard, Vancouver, ISO, and other styles
2

Marotta, Marcelo Hilsdorf. "Sobre o problema da identidade cultural dos artefatos na análise contextual da arte etrusca." In Encontro da História da Arte. Universidade Estadual de Campinas, 2006. http://dx.doi.org/10.20396/eha.2.2006.3874.

Full text
Abstract:
No estudo da arte antiga, uma das questões metodológicas mais importantes se refere à importância da análise contextual para que se possa compreender adequadamente o significado, a função e o valor próprio do documento do passado. Tal questão se torna especialmente relevante quando da necessidade de analisar documentos que, como no caso do mundo antigo, são forjados dentro de contextos dificilmente discerníveis em termos de unidades completamente autônomas entre si, pois o que se tem notado com cada vez mais freqüência nos estudos mais recentes é a existência de tantas e diversificadas “redes de significados” (C. Geertz) que permeiam ou não outras tantas redes de contatos na Antigüidade, que fica muitas vezes praticamente impossível, do ponto de vista metodológico dos significados dos artefatos, de se individuar de forma “clara e distinta” o que seja exatamente um produto de uma cultura em contraposição ao de uma outra cultura, sem que com isso se incorra mais uma vez no preconceito, historicamente formulado, da existência de uma cultura superior e, portanto, central, junto de outras mais periféricas. Pois como distinguir, do ponto de vista do significado, um artefato como sendo superior a outro? Assim, partindo do caso específico da recepção da arte grega pelos Etruscos ao longo do primeiro milênio antes de Cristo, gostaríamos de apresentar, na presente comunicação, algumas considerações gerais de ordem teórico-metodológica sobre a importância da análise contextual e de como podem ser evitados alguns equívocos de cunho eminentemente etnocêntrico na consideração da arte antiga.
APA, Harvard, Vancouver, ISO, and other styles
3

Bussel, J., and E. Rabellino. "HETEROGENEITY OF MEGAKARYOCYTE PLOIDY PROFILE IN PATIENTS WITH ITP." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644580.

Full text
Abstract:
Thrombocytopenia in patients with ITP is ascribed to peripheral platelet destruction mediated by antiplatelet antibodies. Patients with a chronic severe form of disease may also have im paired platelet production. Profile of DNA content and distribu tion in marrow megakaryocytes (MK) is a sensitive parameter of megakaryocyte development. In these studies MK derived from clinical aspirates were analyzed by flow cytometry for DMA content and platelet glycoprotein GP IIb/IIIa. Both whole unsenar-ated and MK-enriched marrow were stained using double label staining for GP IIb-IIIa by immunofluorescence (green) and for DNA with propidium iodide (red). 3 different profiles were seen in ITP patients as indicated in the Table:Profile I represented the commonest pattern and was identical to that seen in normals. Profiles II and III demonstrated peak ploidy values of 8C and a bimodal 2C/16C pattern respectively. In order to investigate the relationship of ploidy profile to platelet production, we compared the peak platelet response to IVGG, a treatment which stops platelet destruction, among the 3 groups. The average platelet response to IVGG was 221,000/ul for group I, much greater than both group II (119k) and group III (96k)(I vs II + III, p < 0.05). Heterogeneity of clinical cases of ITP may be caused by differences in ploidy profile of patient marrow megakaryocytes.These profiles may be caused by a heterogeneity of the target antigen of the antiplatelet antibodies in different patients with differing crossreactivity to and effects on megakaryocytes. This heterogeneity appears to be reflected in different responses to therapies suggesting that it may be useful in designing treatment strategies.
APA, Harvard, Vancouver, ISO, and other styles
4

Klein, Kevin M., Gregory T. Ostrowicki, Andrew Gerwitz, and Suresh K. Sitaraman. "Micro and Nano Thin Film Devices as Bio-Assays for Cancer Diagnosis." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-15581.

Full text
Abstract:
Micro and nano Au/Cr and Al thin film devices have been fabricated using DC sputtering and e-beam evaporation in combination with e-beam and photo lithography. These devices can be coated with specific reagents to detect and measure the presence of particular antigens and/or complementary DNA sequences with a smaller sample size and at much earlier stages of disease progression compared to current medical diagnostic technologies. Using the device material stack (Au/Cr/Si), we have assessed the binding affinity of Au, Cr, and Si with Protein G, and antibodies for Prostate Specific Antigen (PSA) and Cancer Antigen 125 (CA125), an ovarian cancer-associated antigen. Based on our experiments, we see that the thin gold layer of the Au/Cr/Si samples, provides increased bio-material binding affinity, and the chromium layer has a similar, if not less, binding affinity compared to the silicon chip alone.
APA, Harvard, Vancouver, ISO, and other styles
5

Standen, G., P. Moodie, H. Pannekoek, C. L. Verweij, and I. R. Peake. "ANALYSIS OF THE VON WILLEBRAND FACTOR (vWF) GENE IN 6 PATIENTS WITH SEVERE TYPE III VON WILLEBRANDS DISEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644641.

Full text
Abstract:
DNA from 6 unrelated patients with severe type III von Willebrands disease (vWF antigen < 0.01u/dl) was studied with a cDNA probe for the 3' end of the vWF gene. DNA was extracted from peripheral blood leucocytes using standard techniques and was digested with a range of restriction enzymes. DNA fragments were separated by electrophoresis in 0.7% agarose and were southern blotted onto hybond-N (Amersham). The probe used was pvWF1100, a 1.1kb PstI fragment derived from the 2.28kb vWFcDNA insert of pvWF2280 isolated from a human endothelial cell cDNA expression library (Verweij et al, Nucleic Acids Res 13 (1985) 4699-4717). The probe corresponds to nucleotides 7083 to 8191 of the vWF cDNA (first nucleotide of initiator methionine as 1).When digested with Bglll and probed with pvWF11000, normal DNA showed two invariant bands (13 and 4.9kb) and polymorphic bands of 9 and/or 7.4kb. This pattern was also seen in 5 of the 6 severe vWD patients DNA suggesting that in this 3' area of the gene they had no major deletions or rearrangements. In the 6th case however the band of 4.9kb was not seen and did not appear to be replaced by any novel fragments, suggesting a partial deletion including some of the 3' end of the gene. This patient had the clinically severest form of the condition in that the patient had developed, some 10 years ago, an antibody (inhibitor) to vWF as detected by the ability of the patients plasma to inhibit restocetin cofactor activity in normal plasma. His parents were related (his mother was his father's second cousin) and had levels of vWFAg, considerably lower than those of factor VIII activity. This situation has been previously reported in carriers of recessive severe vWD. vWD was also present in a second family member, but in a less severe form (vWFAg 3u/dl). This patient and all other members of the family have, to date, given normal restriction fragment patterns with the vWF probe and several enzymes, including BgIII.
APA, Harvard, Vancouver, ISO, and other styles
6

Yue, Min, Jeanne C. Stachowiak, Henry Lin, Kenneth Castelino, Ram Datar, Karolyn Hansen, Thomas Thundat, Arup Chakraborty, Richard J. Cote, and Arun Majumdar. "Nanomechanical Sensor Array for Detection of Biomolecular Bindings: Toward a Label-Free Clinical Assay for Serum Tumor Markers." In ASME 2004 3rd Integrated Nanosystems Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/nano2004-46034.

Full text
Abstract:
A label-free technique capable of rapidly screening human blood samples simultaneously for multiple serum tumor markers would enable accurate and cost-effective diagnosis of cancer before physiological symptoms appear. Recently, microfabricated, bimaterial cantilever sensors have been demonstrated to detect DNA hybridization and antigen-antibody binding at clinically relevant concentrations. Cantilever sensors deflect measurably under the surface stress resulting when biomolecules immobilized on one surface of the sensor interact with their binding partners [1]. We present an array of cantilever sensors (silicon nitride with a gold coated surface) capable of simultaneously interrogating 100 different biomolecular interactions.
APA, Harvard, Vancouver, ISO, and other styles
7

Hermes, Maria Helena da Fonseca. "O antigo Hotel Balneário Sete de Setembro: arquitetura eclética de tendência clássica." In Encontro da História da Arte. Universidade Estadual de Campinas, 2007. http://dx.doi.org/10.20396/eha.3.2007.3714.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Geddes, V. A., G. V. Louie, G. D. Brayer, and R. T. A. MacGillivray. "MOLECULAR BASIS OF HEMOPHILIA B: IDENTIFICATION OF THE DEFECT IN FACTOR IX VANCOUVER." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643872.

Full text
Abstract:
Factor IX Vancouver (fIX-V) is the cause of a moderate form of hemophilia B. An individual presenting with this disorder had 2.6% of normal procoagulant activity in his plasma but had 62% of the normal factor IX antigen level. Specific antibodies showed that fIX-V contains epitopes for both the heavy and light chains of factor IXa. To identify the defect involved, DNA was isolated from the lymphocytes of the male hemophiliac. Southern blot analysis using a full-length factor IX cDNA as a hybridization probe showed no gross differences between the fIX-V gene and the normal factor IX gene. The DNA from the hemophiliac was then partially digested with Sau3A and the resulting fragments (10-20kbp in size) were ligated into the BamHI site of λEMBL3. The DNA was then packaged into phage particles in vitro, and the recombinant phage were screened with the factor IX cDNA as a probe. Eight phage were isolated that contained overlapping DNA covering the complete gene for fIX-V. DNA sequence analysis of the protein-encoding regions, the intron/exon junctions and 5'-and 3'-flanking sequences revealed a single nucleotide change from the normal factor IX gene. The codon for amino acid 397 was changed from ATA (lie) to ACA (Thr). This mutation is in the catalytic domain of factor IXa and is novel amongst those hemophilia B mutations reported to date. Based on the known three dimensional structures of the pancreatic serine proteases, trypsin, elastase and chymotrypsin, models have been constructed for the structures of the catalytic domains of both the normal and Thr-397 mutant of factor IXa. These results suggest that the Thr-397 mutation may alter the conformation of the substrate binding region in the active site of factor IXa Vancouver through the formation of a hydrogen bond between the hydroxyl group of the Thr-397 side chain and the main chain carbonyl group of Trp-385. The postulated conformational change would lead to reduced binding affinity for the factor IXa substrate resulting in a reduction in the catalytic activity of fIXa-Vancouver.Supported in part by grants from the Medical Research Council of Canada (to GDB and RTAM).
APA, Harvard, Vancouver, ISO, and other styles
9

Viana, Alice de Oliveira, and Marina Bianchi Guaragni. "Reinventando o antigo: John Ruskin e o ornamento assírio." In Encontro de História da Arte. Universidade Estadual de Campinas, 2019. http://dx.doi.org/10.20396/eha.vi14.3369.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Martins, Letícia, Marianny Rodrigues Costa Amorim, and Andreia Juliana Rodrigues Caldeira. "ORIGEM E IMPORTÂNCIA FILOGENÉTICA DO DNA MITOCONDRIAL." In I Congresso Nacional On-line de Biologia Celular e Estrutural. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1942.

Full text
Abstract:
Introdução: A molécula do DNA mitocondrial (mtDNA) é muito utilizada em estudos envolvendo estrutura populacional, relações filogenéticas e o entendimento de vários aspectos biológicos e evolutivos de uma grande variedade de organismos. Mas, apesar desse destaque em estudos moleculares, ainda existem muitas dúvidas sobre a organela. Objetivo: Realizar uma revisão bibliográfica sobre a origem e importância filogenética do mtDNA.Material e método: Foi realizada uma busca de artigo embase dados como SciELO Brasil e Web of Science. Resultados: A mitocôndria é uma organela responsávelpela respiração celular e tem origem endossimbiótica, que pode ser evidenciada pela presença de um DNA própriocircular, semelhante à células ancestrais procariotas. O mtDNA é pequeno (aproximadamente 16 kb nos animais), com raras exceções; possui poucos genes, 37 no total, que codifica para apenas 5% dos produtos necessários para o funcionamento da mitocôndria. É considerado como um genoma compacto, com poucas seqüências espaçadoras, seqüências repetitivas, pseudogenes e introns e aindaausência de recombinação, embora exceções sejam descritas. O conteúdo gênico é conservado, e a ordem em que esses genes se encontram organizados no genomacostuma ser também conservada. A taxa evolutiva do mtDNA é alta, quando comparada a do genoma nuclear. O mtDNA é capaz de ligar pessoas à sua linhagem materna, já que este possui herança exclusivamente materna além disso, é considerado um marcador genético, pois apresenta mais de 5 mil cópias numa única célula. Conclusão: A análise desse tipo de DNA é excepcional em estudo de tecidos antigos e até arqueológicos, como dentes e ossos epodem ser amplamente usados em estudo de evolução e antropologia. Na atualidade, o mtDNA ganhou destaque na área forense, favorecendo a coleta evidencias que elucidam as situações de crimes.
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "DNA antiguo"

1

Middlebrooks, Bobby L. Evaluation of a DNA Vaccine Specific for the 54 kDa Protective Antigen of Erysipelothrix rhusiopathiae. Fort Belvoir, VA: Defense Technical Information Center, December 2006. http://dx.doi.org/10.21236/ada495910.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Middlebrooks, Bobby L. Production of a DNA Vaccine Specific for the 64 kDa Protective Antigen of Erysipelothrix rhusiopathiae. Fort Belvoir, VA: Defense Technical Information Center, February 2007. http://dx.doi.org/10.21236/ada462832.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'DNA antiguo' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography