Log in

Relevant bibliographies by topics / DNA antiguo / Journal articles

To see the other types of publications on this topic, follow the link: DNA antiguo.

Journal articles on the topic 'DNA antiguo'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'DNA antiguo.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Casas-Vargas, Andrea, Liza M. Romero, William Usaquén, Sara Zea, Margarita Silva, Ignacio Briceño, Alberto Gómez, and José Vicente Rodríguez. "Mitochondrial DNA diversity in prehispanic bone remains on the eastern Colombian Andes." Biomédica 37, no. 4 (December 1, 2017): 548. http://dx.doi.org/10.7705/biomedica.v37i4.3377.

Full text

Abstract:

Introducción. El ADN antiguo que se extrae de los restos óseos humanos permite analizar la composición genética de las poblaciones precolombinas y determinar las dinámicas poblacionales que dieron origen a la diversidad de las poblaciones contemporáneas.Objetivo. Determinar la diversidad genética y la relación con otras comunidades contemporáneas y antiguas de América, de los restos óseos asociados al Templo del Sol en Sogamoso, Colombia.Materiales y métodos. Se analizaron 13 individuos pertenecientes al periodo precolombino muisca (siglos IX-XVI d. C.), provenientes de los alrededores del Templo del Sol en Sogamoso, Boyacá, Andes orientales colombianos. Se amplificó el ADN mitocondrial (ADNmt) y se determinaron los polimorfismos de la longitud de los fragmentos de restricción (Restriction Fragment Length Polymorphism, RFLP) para los cuatro haplogrupos amerindios (A, B, C y D). Además, se amplificaron y analizaron los marcadores autosómicos, incluida la amelogenina, y los marcadores de los polimorfismos de repeticiones cortas en tándem (Short Tandem Repeat, STR) del cromosoma Y.Resultados. El haplogrupo A fue el linaje mitocondrial más frecuente en esta población, seguido de los haplogrupos B y C; no se detectó el haplogrupo D. Los análisis de variación genética indicaron una diversidad semejante a la de las poblaciones pertenecientes a la familia lingüística chibcha, contemporánea en Colombia y Centroamérica. Se logró hacer la determinación molecular del sexo de los individuos estudiados y compararla con los datos osteológicos. Con una sola excepción, los datos bioantropológicos y moleculares concordaron.Conclusiones. Estos resultados aportan nuevos elementos a la hipótesis del origen centroamericano de los grupos chibchas del altiplano cundiboyacense con base en marcadores genéticos, y permitieron establecer el sexo y las relaciones de parentesco.

APA, Harvard, Vancouver, ISO, and other styles

2

Sun, Jing, Dingfeng Li, Yanling Hao, Yuwei Zhang, Wenling Fan, Jingjing Fu, Yunzhang Hu, Yong Liu, and Yiming Shao. "Posttranscriptional Regulatory Elements Enhance Antigen Expression and DNA Vaccine Efficacy." DNA and Cell Biology 28, no. 5 (May 2009): 233–40. http://dx.doi.org/10.1089/dna.2009.0862.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Liu, Yang, Zhimin He, Dapeng Feng, Guodong Shi, Rui Gao, Xiaodong Wu, Weiguo Song, and Wen Yuan. "Cytotoxic T-lymphocyte Antigen-4 Polymorphisms and Susceptibility to Osteosarcoma." DNA and Cell Biology 30, no. 12 (December 2011): 1051–55. http://dx.doi.org/10.1089/dna.2011.1269.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

MARESH, GRACE A., JOHN S. MARKEN, MICHAEL NEUBAUER, ALEJANDRO ARUFFO, INGEGERD HELLSTRÖM, KARL ERIK HELLSTRÖM, and HANS MARQUARDT. "Cloning and Expression of the Gene for the Melanoma-Associated ME20 Antigen." DNA and Cell Biology 13, no. 2 (February 1994): 87–95. http://dx.doi.org/10.1089/dna.1994.13.87.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Klemen, Nicholas, Raul Vizcardo, Linda Tran, and Nicholas P. Restifo. "Precocious Differentiation of Somatic and Pluripotent Stem Cells Bearing Pre-Arranged TCR." Blood 126, no. 23 (December 3, 2015): 847. http://dx.doi.org/10.1182/blood.v126.23.847.847.

Full text

Abstract:

Abstract Adoptive cell transfer (ACT) of mutation-specific T lymphocytes may represent the future of immunotherapy for cancer. Current strategies utilize mature T cells isolated from tumors, but this approach may be hampered by limited persistence, advanced differentiation status, and exhaustion of transferred cells. Cellular rejuvenation is needed, and this could be accomplished through induced pluripotent stem cell (iPS) technology. IPS cells can be generated from various types of somatic cells using the four Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc), and we have shown that iPS generated from T cells (T-iPS) retain their original TCR specificity upon re-differentiation into T cell lineage. With existing technology, tumor antigen-specific progenitor cells can be derived from T-iPS. These cells could have superior potential to engraft, proliferate, and persist for long periods of time. To explore this concept, we have generated a murine iPS cell line from our Pmel transgenic mouse model, in which T cells bear a pre-rearranged TCR specific for the melanoma antigen GP100. Differentiation in vitro employs the OP9-DL1 co-culture system. We characterize the DN1-DN4 stages by flow cytometry of T cell lineage differentiation markers, such as c-kit, CD3, CD4, CD8, CD25, TCRvb, and TCRvb13 (present in the Pmel transgene). To study somatic stem cell differentiation, we used lineage negative bone marrow from femurs of wild type C57BL/6 (WT) and Pmel TCR transgenic (Pmel) mice. After 10 days of culture, cells were harvested and analyzed. Frequencies of cells in the DN1-DN4 stages for WT were 2%, 41%, 55%, and 2%; for Pmel they were 5%, 31%, 39%, and 25%. Expression of CD3 in WT cells in the DN1/DN2 stage was 1%, and for the DN3/DN4 stage was 2%. In contrast, CD3 expression in Pmel cells was 85% for cells in DN1/DN2 and 95% for cells in DN3/DN4. To study pluripotent stem cell differentiation, we used WT embryonic stem cells (ES) and Pmel iPS. After 21 days of culture, cells were harvested and analyzed. The double positive compartment was 9% in WT ES and 30% in Pmel iPS; the double negative compartment was 60% and 37%. Frequencies of cells in DN1-DN4 stages for WT ES were 4%, 6%, 45%, and 45%; for Pmel they were 1%, 7%, 69%, and 23%. Expression of CD3 in WT cells in the DN1/DN2 stage was 1%, and for the DN3/DN4 stage was 13%. In contrast, CD3 expression in Pmel cells was 76% for cells in DN1/DN2 and 91% for cells in DN3/DN4. These data show for the first time that in vitro differentiation of pluripotent stem-derived progenitor cells bearing a pre-rearranged TCR will undergo precocious expression of the CD3 molecule and enhanced progression to DP stage as compared to WT controls. The pattern is consistent with that previously seen during in vitro differentiation of somatic stem cells. It appears that the presence of a pre-rearranged TCR is driving precocious development of T cells in vitro, regardless of whether the source of progenitors is from somatic (bone marrow) or pluripotent (ES/iPS) stem cells. Our ultimate goal is to intricately characterize the nature of iPS-derived T cells, and study their antitumor efficiency in vitro and in vivo, in order to evaluate their suitability for clinical use. Disclosures No relevant conflicts of interest to declare.

APA, Harvard, Vancouver, ISO, and other styles

6

Lang, Cuicui, Lei Chen, and Senlin Li. "Cytotoxic T-Lymphocyte Antigen-4 +49G/A Polymorphism and Susceptibility to Pancreatic Cancer." DNA and Cell Biology 31, no. 5 (May 2012): 683–87. http://dx.doi.org/10.1089/dna.2011.1417.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

MURALIKRISHNA, T., ZAREENA BEGUM, Ch V. B. SWAMY, and ASHOK KHAR. "Molecular Cloning and Characterization of a Tumor Rejection Antigen from Rat Histiocytoma, AK-5." DNA and Cell Biology 17, no. 7 (July 1998): 603–12. http://dx.doi.org/10.1089/dna.1998.17.603.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Ou, JianFeng, Kang Li, Hui Ren, Hai Bai, Dan Zeng, and ChongJie Zhang. "Association and Haplotype Analysis of Prostate Stem Cell Antigen with Gastric Cancer in Tibetans." DNA and Cell Biology 29, no. 6 (June 2010): 319–23. http://dx.doi.org/10.1089/dna.2009.0960.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Hassen, Elham, Randa Ghedira, Nahla Ghandri, Karim Farhat, Sallouha Gabbouj, Noureddine Bouaouina, Hamdi Abdelaziz, Abdelatif Nouri, and Lotfi Chouchane. "Lack of Association Between Human Leukocyte Antigen-E Alleles and Nasopharyngeal Carcinoma in Tunisians." DNA and Cell Biology 30, no. 8 (August 2011): 603–9. http://dx.doi.org/10.1089/dna.2010.1140.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

SAWADA, RITSUKO, KENSAKU OHASHI, HIROYUKI ANAGUCHI, HITOAKI OKAZAKI, MASAKAZU HATTORI, NAGAHIRO MINATO, and MASANOBU NARUTO. "Isolation and Expression of the Full-Length cDNA Encoding CD59 Antigen of Human Lymphocytes." DNA and Cell Biology 9, no. 3 (April 1990): 213–20. http://dx.doi.org/10.1089/dna.1990.9.213.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Wen, Yue-Jin, Anne Mancino, Anastas Pashov, Tracy Whitehead, Joseph Stanley, and Thomas Kieber-Emmons. "Antigen Binding of Human IgG Fabs Mediate ERK-Associated Proliferation of Human Breast Cancer Cells." DNA and Cell Biology 24, no. 2 (February 2005): 73–84. http://dx.doi.org/10.1089/dna.2005.24.73.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

LAMPEL, S., J. M. BRIDGER, R. M. ZIRBEL, U. R. MATHIEU, and P. LICHTER. "Nuclear RNA Accumulations Contain Released Transcripts and Exhibit Specific Distributions with Respect to Sm Antigen Foci." DNA and Cell Biology 16, no. 10 (October 1997): 1133–42. http://dx.doi.org/10.1089/dna.1997.16.1133.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Li, Zhongming, Dan Song, Hongyong Zhang, Wei He, Xiaoyong Fan, Ying Zhang, Jialu Huang, Xiaoyi Wang, Qingliang Liu, and Sidong Xiong. "Improved Humoral Immunity Against Tuberculosis ESAT-6 Antigen by Chimeric DNA Prime and Protein Boost Strategy." DNA and Cell Biology 25, no. 1 (January 2006): 25–30. http://dx.doi.org/10.1089/dna.2006.25.25.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Liu, Jun, Miao Liu, Jiling Wang, Weifeng Xu, Wanzun Lin, Weifeng Tang, Yafeng Wang, Jinrong Chen, Jianhua Lin, and Lurong Zhang. "Comparison of Three Different Assays for the Detection of Tumor Antigen-Induced Lymphocyte Transformation In Vitro." DNA and Cell Biology 38, no. 11 (November 1, 2019): 1402–10. http://dx.doi.org/10.1089/dna.2019.4849.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

RENNER, KARIN, ELISABETH SOCK, JOSEF-KARL GERBER, and MICHAEL WEGNER. "T Antigen of Human Papovavirus JC Stimulates Transcription of the POU Domain Factor Tst-1/Oct6/SCIP." DNA and Cell Biology 15, no. 12 (December 1996): 1057–62. http://dx.doi.org/10.1089/dna.1996.15.1057.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Gunes, Sezgin, Hasan Bagci, Saban Sarikaya, Cenk Yucel Bilen, and Nurten Kara. "Prostate-Specific Antigen and 17-Hydroxylase Polymorphic Genotypes in Patients with Prostate Cancer and Benign Prostatic Hyperplasia." DNA and Cell Biology 26, no. 12 (December 2007): 873–78. http://dx.doi.org/10.1089/dna.2007.0646.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Neznanov, Nickolay, Lubov Neznanova, Brigitte Angres, and Andrei V. Gudkov. "Serologically Defined Colon Cancer Antigen 3 Is Necessary for the Presentation of TNF Receptor 1 on Cell Surface." DNA and Cell Biology 24, no. 12 (December 2005): 777–85. http://dx.doi.org/10.1089/dna.2005.24.777.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Zhou, Yujia, Lunxian Tang, Minjia Lin, Shumin Xu, Jianwen Bai, and Haihan Song. "Expression of Cytotoxic T-Lymphocyte Antigen 4 on CD4+ and CD8+ T Cells Is Increased in Acute Lung Injury." DNA and Cell Biology 32, no. 12 (December 2013): 722–26. http://dx.doi.org/10.1089/dna.2013.2112.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

DUTT, KAMLA, MATTIE SCOTT, MIER WANG, EUGENE SEMPLE, GANPATI P. SHARMA, and ALAGARSAMY SRINIVASAN. "Establishment of a Human Retinal Cell Line by Transfection of SV40 T Antigen Gene with Potential to Undergo Neuronal Differentiation." DNA and Cell Biology 13, no. 9 (September 1994): 909–21. http://dx.doi.org/10.1089/dna.1994.13.909.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

CHAN, WAI-YEE, JIMO BORJIGIN, QIAO-XI ZHENG, and W. LESLEY SHUPERT. "Characterization of cDNA Encoding Human Pregnancy-Specific β1-Glycoprotein from Placenta and Extraplacental Tissues and Their Comparison with Carcinoembryonic Antigen." DNA 7, no. 8 (October 1988): 545–55. http://dx.doi.org/10.1089/dna.1.1988.7.545.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

SKOW, LOREN C., JAMES E. WOMACK, J. MARK PETRESH, and WALTER L. MILLER. "Synteny Mapping of the Genes for 21 Steroid Hydroxylase, Alpha A Crystallin, and Class I Bovine Leukocyte Antigen in Cattle." DNA 7, no. 3 (April 1988): 143–49. http://dx.doi.org/10.1089/dna.1988.7.143.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Guijarro Ruano, Paloma, and Fernando García Romero. "Ciudades de esclavos en el Mundo Antiguo: ¿realidad o ficción?" Dialogues d'histoire ancienne 46/1, no. 1 (2020): 69. http://dx.doi.org/10.3917/dha.461.0069.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

WILLIAMS, WILLIAM V., ALICE SATO, MILTON ROSSMAN, QIONG FANG, and DAVID B. WEINER. "Specific DNA Amplification Utilizing the Polymerase Chain Reaction and Random Oligonucleotide Primers: Application to the Analysis of Antigen Receptor Variable Regions." DNA and Cell Biology 11, no. 9 (November 1992): 707–20. http://dx.doi.org/10.1089/dna.1992.11.707.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Yamanaka, Ryuya, and Kleanthis G. Xanthopoulos. "Induction of Antigen-Specific Immune Responses Against Malignant Brain Tumors by Intramuscular Injection of Sindbis DNA Encoding Gp100 and IL-18." DNA and Cell Biology 24, no. 5 (May 2005): 317–24. http://dx.doi.org/10.1089/dna.2005.24.317.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

TEITZ, TAL, T. S. BENEDICT YEN, JUDY C. CHANG, and YUET WAI KAN. "SV40 T Antigen Directed by a Powerful Erythroid Enhancer–Promoter Produced Sarcomas and Pancreatic Tumors But Not Erythroid-Specific Tumors in Transgenic Mice." DNA and Cell Biology 13, no. 7 (July 1994): 705–10. http://dx.doi.org/10.1089/dna.1994.13.705.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Zhang, Ziping, Bingling Shen, Yilei Wang, Yun Chen, Guodong Wang, Peng Lin, and Zhihua Zou. "Molecular Cloning of Proliferating Cell Nuclear Antigen and Its Differential Expression Analysis in the Developing Ovary and Testis of Penaeid Shrimp Marsupenaeus japonicus." DNA and Cell Biology 29, no. 4 (April 2010): 163–70. http://dx.doi.org/10.1089/dna.2009.0958.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

COUTO, JOSEPH R., MICHAEL R. TAYLOR, SIMON G. GODWIN, ROBERTO L. CERIANI, and JERRY A. PETERSON. "Cloning and Sequence Analysis of Human Breast Epithelial Antigen BA46 Reveals an RGD Cell Adhesion Sequence Presented on an Epidermal Growth Factor-Like Domain." DNA and Cell Biology 15, no. 4 (April 1996): 281–86. http://dx.doi.org/10.1089/dna.1996.15.281.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Sellami, Mohamed Hichem, Manel Chaabane, Houda Kaabi, Lamia Torjemane, Saloua Ladeb, Tarek Ben Othmane, and Slama Hmida. "Do FY Antigens Act As Minor Histocompatibility Antigens in the Graft-Versus-Host Disease Paradigm After Human Leukocyte Antigen-Identical Sibling Hematopoietic Stem Cell Transplantation?" DNA and Cell Biology 31, no. 3 (March 2012): 331–36. http://dx.doi.org/10.1089/dna.2011.1321.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Amirulloh, Dian, Silvia Tri Widyaningtyas, and Budiman Bela. "Konstruksi Plasmid Pengekspresi Antigen Rekombinan HCV Berbasis Multiepitop untuk Deteksi Antibodi Anti-HCV." Media Penelitian dan Pengembangan Kesehatan 28, no. 3 (December 3, 2018): 175–82. http://dx.doi.org/10.22435/mpk.v28i3.39.

Full text

Abstract:

AbstractHepatitis C virus (HCV) infection can cause chronic liver disease that develops into cirrhosis and liver cancer. It is estimated that are more than 170 million of th world’s population suffering from HCV. Accurate diagnosis is needed to provide appropriate early treatmen, including preventing further transmission of the virus. The purpose of this study was to construct plasmid expression of recombinant antigen for detection of anti-HCV antibodies. The antigen coding gene is designed so that is composed to epitopes that are immunodominant, sustainable and and represent HCV subtypes circulating in Indonesia and globally. Furthermore, the gene was made by synthetic DNA techniques by DNA synthesis service providers and accepted by the researchers in the form of blinding on the PUC57 plasmid to pQE80L plasmid with BamHI and HindIII cloning sites. Subcloned recombinant plasmids were then propagated on Top10 Escherichia coli cells and verified by PCR colony tecnique, restriction, and sequencing analysis. HCV recombinant antigen coding gene is 1200 bp. Cloning of these gene on the PUC57 vector produced a plasmid pUC57-HCV_ME (3910 bp) and subcloned in the pQE80L vector producing pQE80L-HCV_ME plasmid (5909bp). Based on verification results of pQE80L-HCV_ME plasmid the expression of recombinant antigen for detection of anti-HCV antibodies has been successfully constructed. AbstrakInfeksi hepatitis C virus (HCV) dapat menyebabkan penyakit hati kronis yang berkembang menjadi sirosis dan kanker hati. Diperkirakan terdapat lebih dari 170 juta penduduk dunia menderita HCV. Diagnosis yang akurat diperlukan untuk memberikan penanganan tepat secara dini, termasuk mencegah penularan virus tersebut lebih lanjut. Tujuan penelitian ini adalah mengonstruksi plasmid pengekspresi antigen rekombinan untuk deteksi antibodi anti-HCV. Gen pengode antigen tersebut dirancang sedemikian rupa sehingga tersusun atas epitop yang bersifat imunodominan, lestari, serta mewakili subtipe HCV yang bersirkulasi di Indonesia maupun global. Selanjutnya gen tersebut dibuat dengan teknik DNA sintetik oleh perusahaan penyedia jasa sintesis DNA dan diterima oleh peneliti dalam bentuk terklona pada plasmid pUC57. Untuk ekspresi pada sel Escherichia coli, gen penyandi antigen rekombinan disubklona dari plasmid pUC57 ke plasmid pQE80L dengan situs pengklonaan BamHI dan HindIII. Plasmid rekombinan hasil subklona kemudian dipropagasi pada sel Escherichia coli Top10 dan diverifikasi dengan teknik PCR koloni, analisis dengan enzim restriksi dan sekuensing. Gen penyandi antigen rekombinan HCV berbasis epitop multipel (HCV_ME) berukuran 1200 pb. Pengklonaan gen tersebut pada vektor pUC57 menghasilkan plasmid pUC57-HCV_ME (3910 pb) dan subklona pada vektor pQE80L menghasilkan plasmid pQE80L-HCV_ME (5909 pb). Berdasarkan pada hasil verifikasi plasmid pQE80L-HCV_ME pengekspresi antigen rekombinan untuk deteksi antibodi anti-HCV telah berhasil dikonstruksi.

APA, Harvard, Vancouver, ISO, and other styles

30

Mirón Perez, Maria Dolores. "Eirene : Divinidad, género y paz en Grecia antigua." Dialogues d'histoire ancienne 30, no. 2 (2004): 9–31. http://dx.doi.org/10.3406/dha.2004.2678.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Han, Thomas K., and My Lien Dao. "Differential Immunogenicity of a DNA Vaccine Containing theStreptococcus mutansWall-Associated Protein A Gene Versus That Containing a Truncated Derivative Antigen A Lacking in the Hydrophobic Carboxyterminal Region." DNA and Cell Biology 24, no. 9 (September 2005): 574–81. http://dx.doi.org/10.1089/dna.2005.24.574.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Liu, Hui, Xiaolong Song, Junqing Hou, Zhenhua Zhao, and Junkai Chang. "Posttranscriptional Regulation of Human Antigen R by miR-133b Enhances Docetaxel Cytotoxicity Through the Inhibition of ATP-Binding Cassette Subfamily G Member 2 in Prostate Cancer Cells." DNA and Cell Biology 37, no. 3 (March 2018): 210–19. http://dx.doi.org/10.1089/dna.2017.3940.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

DONNELLY, JOHN J, MARGARET A LIU, and JEFFREY B ULMER. "Antigen Presentation and DNA Vaccines." American Journal of Respiratory and Critical Care Medicine 162, supplement_3 (October 2000): S190—S193. http://dx.doi.org/10.1164/ajrccm.162.supplement_3.15tac10.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Kachakova, Darina, Atanaska Mitkova, Elenko Popov, Ivan Popov, Alexandrina Vlahova, Tihomir Dikov, Svetlana Christova, Vanio Mitev, Chavdar Slavov, and Radka Kaneva. "Combinations of Serum Prostate-Specific Antigen and Plasma Expression Levels of let-7c, miR-30c, miR-141, and miR-375 as Potential Better Diagnostic Biomarkers for Prostate Cancer." DNA and Cell Biology 34, no. 3 (March 2015): 189–200. http://dx.doi.org/10.1089/dna.2014.2663.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Yanti, Yanti, Ida Parwati, Agnes Rengga Indrati, and Anggraini Alam. "Validitas Pemeriksaan Antigen P24 HIV Metode Rapid Immunochromatography Terhadap Viral Load RNA HIV Metode PCR." Sari Pediatri 16, no. 5 (November 9, 2016): 347. http://dx.doi.org/10.14238/sp16.5.2015.347-50.

Full text

Abstract:

Latar belakang. Bayi yang dilahirkan oleh ibu pengidap HIV/AIDS akan mengandung antibodi HIV ibudalam darahnya, terdeteksi sampai usia 18 bulan. Pemeriksaan virologi (RNA/DNA HIV dan antigen p24HIV) sesuai standar WHO adalah pemeriksaan HIV pada bayi dan anak <18 bulan terlahir dari ibu HIV/AIDS.Tujuan. Mengetahui validitas pemeriksaan antigen p24 HIV metode rapid immunochromatogrpahy terhadapviral load RNA HIV metode PCR pada bayi dan anak <18 bulan dengan ibu HIV/AIDS.Metode. Penelitian dilakukan di RSUP dr. Hasan Sadikin Bandung dan RSK Dharmais Jakarta, periodeApril-September 2013. Subjek penelitian adalah 72 bayi dan anak berusia <18 bulan yang lahir dari ibuHIV/AIDS.Hasil. Sembilan (12,5%) dari 72 subjek penelitian terdeteksi HIV pada pemeriksaan viral load RNA HIV, 2(22,2%) di antaranya positif pada pemeriksaan antigen p24 HIV. Didapatkan sensitivitas 22,2%, spesifisitas100%, dan akurasi 90,3%.Kesimpulan. Pemeriksaan antigen p24 HIV metode rapid immunochromatography memiliki spesifisitastinggi, sensitivitas rendah sehingga pemeriksaan antigen p24 HIV metode rapid immunohromatographyini dapat digunakan sebagai alat diagnostik.

APA, Harvard, Vancouver, ISO, and other styles

36

Smale, S. T., and R. Tjian. "T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication." Molecular and Cellular Biology 6, no. 11 (November 1986): 4077–87. http://dx.doi.org/10.1128/mcb.6.11.4077.

Full text

Abstract:

We have combined in vitro DNA replication reactions and immunological techniques to analyze biochemical interactions between simian virus (SV40) large T antigen and components of the cellular replication apparatus. First, in vitro SV40 DNA replication was characterized with specific origin mutants. Next, monoclonal antibodies were used to demonstrate that a specific domain of T antigen formed a complex with cellular DNA polymerase alpha. Several antibodies were identified that coprecipitated T antigen and DNA polymerase alpha, while others were found to selectively prevent this interaction and concomitantly inhibit DNA replication. DNA polymerase alpha also bound efficiently to a T-antigen affinity column, confirming the immunoprecipitation results and providing a useful method for purification of the complete protein complex. Taken together, these results suggest that the T-antigen-polymerase association may be a key step in the initiation of SV40 DNA replication.

APA, Harvard, Vancouver, ISO, and other styles

37

Smale, S. T., and R. Tjian. "T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication." Molecular and Cellular Biology 6, no. 11 (November 1986): 4077–87. http://dx.doi.org/10.1128/mcb.6.11.4077-4087.1986.

Full text

Abstract:

We have combined in vitro DNA replication reactions and immunological techniques to analyze biochemical interactions between simian virus (SV40) large T antigen and components of the cellular replication apparatus. First, in vitro SV40 DNA replication was characterized with specific origin mutants. Next, monoclonal antibodies were used to demonstrate that a specific domain of T antigen formed a complex with cellular DNA polymerase alpha. Several antibodies were identified that coprecipitated T antigen and DNA polymerase alpha, while others were found to selectively prevent this interaction and concomitantly inhibit DNA replication. DNA polymerase alpha also bound efficiently to a T-antigen affinity column, confirming the immunoprecipitation results and providing a useful method for purification of the complete protein complex. Taken together, these results suggest that the T-antigen-polymerase association may be a key step in the initiation of SV40 DNA replication.

APA, Harvard, Vancouver, ISO, and other styles

38

He, Guangzhi, Sijie Chen, Tao Wang, Yubo Yan, Zhihe Zhang, Desheng Li, Hua Yu, et al. "Sequence Analysis of the Bs-Ag1 Gene of Baylisascaris schroederi from the Giant Panda and an Evaluation of the Efficacy of a Recombinant Baylisascaris schroederi Bs-Ag1 Antigen in Mice." DNA and Cell Biology 31, no. 7 (July 2012): 1174–81. http://dx.doi.org/10.1089/dna.2011.1395.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Denis, D., and P. A. Bullock. "Primer-DNA formation during simian virus 40 DNA replication in vitro." Molecular and Cellular Biology 13, no. 5 (May 1993): 2882–90. http://dx.doi.org/10.1128/mcb.13.5.2882.

Full text

Abstract:

Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. Initial experiments indicated that T antigen and the polymerase alpha-primase complex are required to form primer-DNA. Proliferating cell nuclear antigen, and presumably proliferating cell nuclear antigen-dependent polymerases, is not needed to form this species. Herein, we present an investigation of the stages at which primer-DNA functions during SV40 DNA replication in vitro. Hybridization studies indicate that primer-DNA is initially formed in the origin region and is subsequently synthesized in regions distal to the origin. At all time points, primer-DNA is synthesized from templates for lagging-strand DNA replication. These studies indicate that primer-DNA functions during both initiation and elongation stages of SV40 DNA synthesis. Results of additional experiments suggesting a precursor-product relationship between formation of primer-DNA and Okazaki fragments are presented.

APA, Harvard, Vancouver, ISO, and other styles

40

Denis, D., and P. A. Bullock. "Primer-DNA formation during simian virus 40 DNA replication in vitro." Molecular and Cellular Biology 13, no. 5 (May 1993): 2882–90. http://dx.doi.org/10.1128/mcb.13.5.2882-2890.1993.

Full text

Abstract:

Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. Initial experiments indicated that T antigen and the polymerase alpha-primase complex are required to form primer-DNA. Proliferating cell nuclear antigen, and presumably proliferating cell nuclear antigen-dependent polymerases, is not needed to form this species. Herein, we present an investigation of the stages at which primer-DNA functions during SV40 DNA replication in vitro. Hybridization studies indicate that primer-DNA is initially formed in the origin region and is subsequently synthesized in regions distal to the origin. At all time points, primer-DNA is synthesized from templates for lagging-strand DNA replication. These studies indicate that primer-DNA functions during both initiation and elongation stages of SV40 DNA synthesis. Results of additional experiments suggesting a precursor-product relationship between formation of primer-DNA and Okazaki fragments are presented.

APA, Harvard, Vancouver, ISO, and other styles

41

Wanner, Dieter. "Review of del Valle (1996): El trueque s/x en el espanol antiguo: Aproximaciones teóricas." Diachronica 15, no. 1 (January 1, 1998): 157–60. http://dx.doi.org/10.1075/dia.15.1.08wan.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Vangasseri, Dileep, Su-Ji Han, and Leaf Huang. "Lipid-Protamine-DNA-Mediated Antigen Delivery." Current Drug Delivery 2, no. 4 (October 1, 2005): 401–6. http://dx.doi.org/10.2174/156720105774370168.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

DAEMEN, T., A. DEMARE, L. BUNGENER, J. DEJONGE, A. HUCKRIEDE, and J. WILSCHUT. "Virosomes for antigen and DNA delivery." Advanced Drug Delivery Reviews 57, no. 3 (January 10, 2005): 451–63. http://dx.doi.org/10.1016/j.addr.2004.09.005.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Bermejo Barrera, José Carlos. "Historia antigua : ¿ Para qué ? Vigor y decadencia de la tradición clásica." Dialogues d'histoire ancienne 29, no. 2 (2003): 29–56. http://dx.doi.org/10.3406/dha.2003.1561.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Utami, Binar R., Betty Agustina T, and Suprapto Ma’at. "DIFFERENCES OF LYMPHOCYTE PROLIFERATION INDEX AFTER CULTURE FILTRATE PROTEIN 10 (CFP-10) STIMULATION IN PATIENTS WITH ACTIVE AND LATENT TUBERCULOSIS AND HEALTHY INDIVIDUALS." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 23, no. 2 (March 29, 2018): 184. http://dx.doi.org/10.24293/ijcpml.v23i2.1144.

Full text

Abstract:

Angka kejadian tuberkulosis di dunia semakin meningkat, akibat rendahnya ketepatgunaan vaksin BCG untuk pencegahan infeksi TB.Saat ini telah dikembangkan vaksin DNA dari protein M.tuberculosis yaitu Culture filtrate protein-10 (CFP-10) yang dapat merangsangrespons imun seluler. Tujuan penelitian ini adalah mengetahui salah satu kemampuan antigenik antigen CFP-10 pasca stimulasi CFP-10 di pasien TB aktif, TB laten dan orang sehat. Penelitian bersifat eksperimen semu (quasi experimental). Sampel penelitian adalahPeripheral blood mononuclear cell (PBMC) dari 10 pasien TB paru aktif baru, 10 TB laten RS Khusus Paru Surabaya dan 10 orangsehat. Perlakuan kultur PBMC tanpa stimulasi (pembanding), dengan Mitogen (PHA) sebagai pembanding positif dan dengan stimulasiantigen CFP-10 diinkubasi pada CO2 5% selama 5 hari. Uji proliferasi limfosit dilakukan dengan menambahkan MTT. Indeks proliferasilimfosit adalah rasio antara absorban pembanding dan absorban dengan stimulasi pada λ 560 nm. Terdapat perbedaan bermakna indeksuji proliferasi limfosit pascastimulasi CFP-10 antara pasien TB paru aktif dan orang sehat (p=0,019) dan di pasien TB aktif, TB latendan orang sehat (p=0,0356). Antigen CFP 10 mampu merangsang respons imun protektif pada pasien TB aktif, TB laten dan orangsehat. Aktivitas imunogenik antigen CFP-10 dapat dipengaruhi oleh status imun seseorang dan kemampuan antigen CFP menginduksirespons imun protektif.

APA, Harvard, Vancouver, ISO, and other styles

46

Mao, Chih-Ping, Chien-Fu Hung, Tae Heung Kang, Liangmei He, Ya-Chea Tsai, Chao-Yi Wu, and T. C. Wu. "Combined Administration with DNA Encoding Vesicular Stomatitis Virus G Protein Enhances DNA Vaccine Potency." Journal of Virology 84, no. 5 (December 16, 2009): 2331–39. http://dx.doi.org/10.1128/jvi.01954-09.

Full text

Abstract:

ABSTRACT DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8+ T-cell responses. We report here a novel concept for DNA vaccine design that exploits the unique and powerful ability of viral fusogenic membrane glycoproteins (FMGs) to couple concentrated antigen transfer to dendritic cells (DCs) with local induction of the acute inflammatory response. Intramuscular administration into mice by electroporation technology of a plasmid containing the FMG gene from vesicular stomatitis virus (VSV-G)—together with DNA encoding the E7 protein of human papillomavirus type 16, a model cervical cancer antigen—elicited robust E7-specific CD8+ T-cell responses, as well as therapeutic control of E7-expressing tumors. This effect could potentially be mediated through the immunogenic form of cellular fusion and necrosis induced by VSV-G, which in a concerted fashion provokes leukocyte infiltration into the inoculation site, enhances cross-presentation of antigen to DCs, and stimulates them to mature efficiently. Thus, the incorporation of FMGs into DNA vaccines holds promise for the successful control of viral infections and cancers in the clinic.

APA, Harvard, Vancouver, ISO, and other styles

47

Umeki, Yuka, Masaaki Saito, Yuki Takahashi, Yoshinobu Takakura, and Makiya Nishikawa. "Retardation of Antigen Release from DNA Hydrogel Using Cholesterol-Modified DNA for Increased Antigen-Specific Immune Response." Advanced Healthcare Materials 6, no. 20 (July 20, 2017): 1700355. http://dx.doi.org/10.1002/adhm.201700355.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Maulani, Tuti Rostianti, Hadi Susilo, Marlinda Indriati, and A. Suhaemi. "Deteksi Cemaran DNA Babi Dengan RT-PCR Pada Sosis Tanpa Logo Halal MUI Dari Empat Kecamatan di Kabupaten Pandeglang Banten." Gorontalo Agriculture Technology Journal 3, no. 2 (October 24, 2020): 72. http://dx.doi.org/10.32662/gatj.v3i2.1171.

Full text

Abstract:

Sosis merupakan produk olahan daging yang cukup digemari di masyarakat khususnya di Kabupaten Pandeglang Provinsi Banten. Pentingnya sertifikat halal atau adanya logo halal MUI (Majelis Ulama Indonesia) untuk produk olahan daging membuat masyarakat muslim yakin untuk mengkonsumsinya. Tujuan dari penelitian ini adalah identifikasi cemaran DNA babi pada produk sosis yang beredar di 4 kecamatan di Wilayah Kabupaten Pandeglang tanpa logo halal MUI menggunakan RT-PCR (Real Time- Polymerase Chain Reaction) dan Pork Detection Kit. Metode dalam penelitan ini diawali dengan menggunakan Pork Detection Kit untuk pengujian antigen babi sebagai kontrol positif dan daging sapi sebagai kontrol negatif yang akan digunakan untuk running RT- PCR. Hasil running RT- PCR terhadap sampel DNA sosis di wilayah 4 (empat) kecamatan di Kabupaten Pandeglang (Majasari, Picung, Munjul, Cimanuk) tanpa label halal MUI menunjukkan trend DNA kontrol negatif pada kurva amplifaksi RT- PCR. Hal ini teridentifikasi bahwa sampel DNA sosis tanpa label halal MUI di 4 kecamatan di Kabupaten Pandeglang tidak tercemar DNA babi

APA, Harvard, Vancouver, ISO, and other styles

49

Rubiera Cancelas, Carla. "Esclavitud femenina en la Roma antigua. Entre la reproducción biológica y la maternidad." Dialogues d'histoire ancienne 41/2, no. 2 (2015): 151. http://dx.doi.org/10.3917/dha.412.0151.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Collins, K. L., and T. J. Kelly. "Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase alpha-primase." Molecular and Cellular Biology 11, no. 4 (April 1991): 2108–15. http://dx.doi.org/10.1128/mcb.11.4.2108.

Full text

Abstract:

Studies of simian virus 40 (SV40) DNA replication in a reconstituted cell-free system have established that T antigen and two cellular replication proteins, replication protein A (RP-A) and DNA polymerase alpha-primase complex, are necessary and sufficient for initiation of DNA synthesis on duplex templates containing the SV40 origin of DNA replication. To better understand the mechanism of initiation of DNA synthesis, we analyzed the functional interactions of T antigen, RP-A, and DNA polymerase alpha-primase on model single-stranded DNA templates. Purified DNA polymerase alpha-primase was capable of initiating DNA synthesis de novo on unprimed single-stranded DNA templates. This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain. We observed that the synthesis of ribonucleotide primers by DNA polymerase alpha-primase is dramatically stimulated by SV40 T antigen. The presence of T antigen also increased the average length of the DNA product synthesized on primed and unprimed single-stranded DNA templates. These stimulatory effects of T antigen required direct contact with DNA polymerase alpha-primase complex and were most marked at low template and polymerase concentrations. We also observed that the single-stranded DNA binding protein, RP-A, strongly inhibits the primase activity of DNA polymerase alpha-primase, probably by blocking access of the enzyme to the template. T antigen partially reversed the inhibition caused by RP-A. Our data support a model in which DNA priming is mediated by a complex between T antigen and DNA polymerase alpha-primase with the template, while RP-A acts to suppress nonspecific priming events.

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!