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Dissertations / Theses on the topic 'DNA aptamers'

Author: Grafiati

Published: 4 June 2021

Last updated: 28 January 2023

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1

Mejri, Nawel. "Development of biosensors based on DNA aptamers for direct mycotoxins detection." Thesis, Perpignan, 2016. http://www.theses.fr/2016PERP0010.

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Le travail réalisé au cours de cette thèse a porté sur le développement de biocapteurs électrochimiques d’affinité, sensibles et sélectifs, pour la détection de l’ochratoxine A (OTA) et l’aflatoxine M1 (AFM1). Les biocapteurs développés reposent sur l’association de différents nanomatériaux pour une meilleure performance analytique. Pour construire notre transducteur, nous avons associé le polypyrrole à des dendrimères poly(amido-amine) PAMAM, ce qui a permis d’avoir de très bon rendements grâce au propriétés électriques du polypyrrole et à l’augmentation de la surface active due à la structur

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2

Marquardt, Janice Dionne. "Force interaction characterization between thrombin and DNA aptamers." [Ames, Iowa : Iowa State University], 2008.

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3

Bayrac, Abdullah Tahir. "In Vitro Selection Of Dna Aptamers To Glioblastoma Multiforme." Phd thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613769/index.pdf.

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Aptamer probes for specific recognition of glioblastoma multiforme were generated using a repetitive and broad cell-SELEX-based procedure without negative selection. The 454 sequencing technology was used to monitor SELEX, and bioinformatics tools were used to identify aptamers from high throughput data. A group of aptamers were generated that can bind to target cells specifically with dissociation constants (K d ) in the nanomolar range. Selected aptamers showed high affinity to different types of glioblastoma cell lines, while showing little or

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4

Kärkkäinen, Riikka M. "Production of DNA aptamers with specificity for bacterial food pathogens." Thesis, University of Chester, 2012. http://hdl.handle.net/10034/620695.

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Aptamers are biomolecular ligands composed of nucleic acids. They can be selected to bind specifically to a range of target molecules and subsequently exploited in a fashion analogous to more traditional biomolecules such as antibodies. In this study a method for selecting new aptamers which specifically bind whole live bacterial cells is described. A non-pathogenic strain of Escherichia coli K12 was used to develop the method. A DNA library containing 100 bases long random nucleotide sequences was produced and the aptamer selection process was repeated nine times. An enzyme-linked technique w

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5

Joseph, Diego F., Jose A. Nakamoto, Ruiz Oscar Andree Garcia, et al. "DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum." Public Library of Science, 2019. http://hdl.handle.net/10757/655484.

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Rapid Diagnostic Tests (RDTs) for malaria are restricted to a few biomarkers and antibody-mediated detection. However, the expression of commonly used biomarkers varies geographically and the sensibility of immunodetection can be affected by batch-to-batch differences or limited thermal stability. In this study we aimed to overcome these limitations by identifying a potential biomarker and by developing molecular sensors based on aptamer technology. Using gene expression databases, ribosome profiling analysis, and structural modeling, we find that the High Mobility Group Box 1 protein (HMGB1)

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6

Lu, Chenze. "Nano-assemblages d'ADN induites par des cibles - Détection de petites cibles par formation de réseaux d'ADN." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAV066/document.

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La détection de petites molécules contribue au développement de nombreux domaines tels que la sécurité alimentaire, la sécurité intérieure, le diagnostic, le contrôle de l'environnement, etc. Cependant, la petite taille de ces cibles et leur faible concentration rendent difficile leur détection. Pour pallier à cela, des biocapteurs avec des sondes appropriées et des stratégies d'amplification du signal sont nécessaires. Parmi les éléments de reconnaissance couramment utilisés, les aptamères présentent l'avantage d'une synthèse aisée et de grandes possibilités de modification, ainsi qu'une déna

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7

Chumphukam, Orada. "Proximity dependent ligation selection : a new approach to generating DNA aptamers." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/24836.

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Nucleic acid aptamers are a group of molecules which emerge from large random sequence pools through in vitro selection, a process called Systematic Evolution of Ligands by EXponential enrichment (SELEX). These recognition molecules are a potential alternative to antibodies and offer a greater thermal stability, robustness and chemical versatility. The first part of this thesis describes the selection of an aptamer against acetylcholinesterase (AChE) using conventional in vitro selection process. The pool enrichment was observed during 15 rounds of selection and a chosen aptamer, R15/19, was c

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8

Lin, Ying [Verfasser]. "Isolation and characterization of DNA aptamers for zinc finger proteins / Ying Lin." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1023581051/34.

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9

Bunka, David Harry John. "Isolation and characterisation of RNA aptamers against DNA binding domains and amyloid." Thesis, University of Leeds, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275670.

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10

Shahidi, Hamedani Nasim [Verfasser]. "Functional Modulation of Activated Protein C using DNA-Aptamers / Nasim Shahidi Hamedani." Bonn : Universitäts- und Landesbibliothek Bonn, 2017. http://d-nb.info/1130704696/34.

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11

Shum, Ka-to, and 沈家滔. "A comparative study of G-quadruplex aptamers against multiple protein targets." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45816232.

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12

Baldock, Brandi. "Impact of Ligand Shell Architecture on Structure and Reactivity of DNA Aptamer-Linked Gold Nanoparticle Assemblies." Thesis, University of Oregon, 2016. http://hdl.handle.net/1794/20400.

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DNA-functionalized gold nanoparticles (DNA-NPs) have enormous potential as building blocks for materials due to their ability to specifically recognize and respond to target molecules and surfaces. The ability of DNA aptamers to adopt different conformations and bind either complementary DNA sequences or analyte molecules allows them to mediate nanoparticle assembly or disassembly, generating selective colorimetric responses. Aptamer-mediated nanoparticle assembly and disassembly is sensitive to the nanoparticle ligand shell composition and structure, yet these topics have not been extensi

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13

Susevski, Vanessa. "Development of DNA Aptamers Targeting Breast Cancer Derived Extracellular Vesicles for Biomarker Discovery." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41035.

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Detection of cancer at the early stages greatly increases the chance for successful treatment and favourable prognosis for patients. However, a liquid-based biopsy has yet to be developed for most cancers. Extracellular vesicles (EVs) are an attractive candidate for early cancer detection since their surface proteome mirrors the cell of origin. Thus, there is a need for the development of reliable probes that can detect cancer derived EVs. In this thesis, the VBS-1 aptamer was developed to selectively bind to triple-negative breast cancer cell line derived EVs. Initially, several EV isolation

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14

Sefah, Kwame. "Development of deoxyribonucleic acid (DNA) aptamers as effective molecular probes for cancer study." [Gainesville, Fla.] : University of Florida, 2009. http://purl.fcla.edu/fcla/etd/UFE0041196.

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15

Tapp, Maeling Janelle Nicole. "Competition-induced selection of ligands for the screening of DNA aptamers for gold substrates." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54851.

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This dissertation presents the development of an alternative aptamer screening process, Competition-Induced Selection of Ligands (CISL), and its use in screening for ssDNA aptamers for gold substrates. Gold substrates are presented as the nonnucleotide target for implementing CISL as a novel aptamer screening approach. Chapter 1 provides an overview of the in vitro selection of oligonucleotide aptamers, the polymerase chain reaction that is a key step in the aptamer screening process, the synthesis and properties of gold nanoparticles and the biomolecule-mediated formation of inorganic nanopar

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16

Martin, Darius Riziki. "The identification of aptamers against serum biomarkers of human tuberculosis." University of the Western Cape, 2018. http://hdl.handle.net/11394/6778.

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>Magister Scientiae - MSc<br>Tuberculosis (TB) is a global health problem and rated as the second leading cause of death after HIV/AIDS. Transmission of TB from one person to the next is very rapid in crowded communities. Therefore, it is crucial to identify people who are infected as quickly as possible not only to provide treatment but also to prevent the spread of the disease. Current TB diagnostic tests such as the culture and sputum smear tests are time-consuming, while rapid tests make use of antibodies that are costly and have low sensitivity and stability. Great improvement has been ob

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17

Dunaway, Adam Blake. "Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets." Thesis, Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/54310.

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Deoxyribonucleic acid (DNA) aptamers are oligonucleotides with high specificity and affinity for non-nucleotide targets ranging from molecular species to cellular proteins. Their high affinity, rapid synthesis, and the ease with which they can be chemically modified to include convenient chemical groups (e.g. amine group on 5’ end) make them excellent adaptable ligands for use in colloidal drug delivery vehicles for both uptake and release of therapeutic agents. This work uses pre-identified aptamers for vascular endothelial growth factor (VEGF) to investigate the design of one such vehicle fo

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18

Ouellet, Eric. "Advanced technologies for improved discovery of DNA aptamers and characterization of biologic affinity reagents." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/54150.

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Biological reagents that recognize target molecules with high affinity and specificity are widely used as capture agents, diagnostic reagents, and therapeutics. Through their ability to adopt structures that confer binding affinity for a target, aptamers represent one major class of such reagents. However, their use is limited by the general inability of current selection methods to reliably discover high-quality aptamers. Inefficiencies in their selection are due in part to a lack of fundamental understanding of the mechanisms underpinning each step in the screening process. This thesis

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19

Gowland, Robert Michael Nicholas. "Directed molecular evolution of novel DNA aptamers raised against an antibiotic resistant Escherichia coli." Thesis, University of Portsmouth, 2015. https://researchportal.port.ac.uk/portal/en/theses/directed-molecular-evolution-of-novel-dna-aptamers-raised-against-an-antibiotic-resistant-escherichia-coli(abc4373e-0b8d-43f4-a106-bef9cd1f04cd).html.

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Antimicrobial resistance presents a serious, worldwide threat to public health. New tools are required to combat the evolving problem. Here we report the systematic evolution of nucleic acid ligands to live Escherichia coli cells. Two SELEX methods were used to generate aptamers to the HB101: pAT153 strain of antibiotic resistant bacterial cells. These different Cell-SELEX methods were compared to analyse how DNA adapts to selective evolutionary pressure. The first method was performed using asymmetric PCR as the mechanism of single strand regeneration, with target cells taken from bacterial c

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20

Makwana, Vaidehi. "Development of novel antitumour therapeutics based on DNA aptamers against the MUC1/Y protein." Thesis, Open University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580135.

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Alterations in the expression of vital proteins of the cellular signalling pathways are at the forefront of molecular abnormalities established in cancer. Therefore, to understand the fundamental mechanisms of cancer initiation and progression, characterisation of tumour markers, such as key proteins like MUCIN that are highly expressed in malignant cells, is crucially important. Rapid drug discovery is a pressing need in the pharmaceutical industry. Agents that are able to bind tightly and selectively to the surface of diseased cells would greatly benefit both disease diagnosis and treatment.

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Zamorskii, I. I. "Antithrombin DNA aptamers as a renoprotective agents against the rhabdomyolysis-induced acute kidney injury." Thesis, БДМУ, 2020. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/18254.

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22

Slater, Nataliya. "In vitro selection of DNA aptamers that neutralise autoantibodies to cytosolic 5’-nucleotidase-1A." Thesis, Slater, Nataliya (2019) In vitro selection of DNA aptamers that neutralise autoantibodies to cytosolic 5’-nucleotidase-1A. Honours thesis, Murdoch University, 2019. https://researchrepository.murdoch.edu.au/id/eprint/54117/.

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Background. Inclusion Body Myositis (IBM) is the most commonly diagnosed inflammatory muscle disease associated with aging. It’s characterised by degeneration of skeletal muscles that inevitably leads to the loss of mobility. The mechanisms that drive IBM pathogenicity remain poorly understood. However, studies reveal that on average 50% of IBM sera contains circulating autoantibodies targeting cytosolic 5’-nucleotidase 1A (cN1A) – a metabolic enzyme that is highly expressed in skeletal muscles. Accumulating evidence suggests that anti-cN1A antibodies may play a role in IBM pathogenesis making

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23

Miller, Emily W. "Generation of modified DNA aptamers toward a complex whole cell target and investigations into improving selection methodology with modified DNA." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/54278.

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Since the discovery was made that DNA is capable of functions beyond genetic storage and propagation, much work has been done to explore the non-canonical abilities of this molecule. Two functionalities of DNA are of particular interest. The first is the ability of DNA to act as a catalyst, and the second is the ability of the molecule to bind with high affinity to various biological and non-biological surfaces. Although numerous DNA catalysts and binders (termed “aptamers”) have been identified using the five nucleoside triphosphates found in nature, the functionality of the molecule can be e

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Tucker, Wesley Owen. "Towards specific DNA aptamers which bind and inhibit WWP1 HECT ubiquitin ligase in the osteoblast." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/205640.

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DNA aptamers have been studied since their inception in 1990, but have only targeted membrane and serum proteins in therapeutics. Their potential as inhibitors of protein function is hampered by their inability to efficiently enter cells in order to function. Surmounting this hurdle is worthwhile since the inhibition of protein-protein interactions is not achievable by small molecule pharmaceuticals alone. Herein we target an intracellular ubiquitin ligase WWP1, which is known to complex with Schnurri3 and polyubiquitinate Runx2, thus targeting it for proteosomal destruction. Since Runx2 is

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Amos-Brown, Bianca. "Isolation and characterisation of novel DNA aptamers against Mycobacterium tuberculosis biomarkers: new tools for tuberculosis diagnostics." Doctoral thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29452.

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Tuberculosis is a curable disease with an average treatment success rate of 86 %. Despite this, there were an estimated 1.5 million deaths due to tuberculosis in 2013, most of which occurred in low and middle income countries. In order to overcome tuberculosis in developing countries innovation in diagnostics is key to administering treatment. While detection of whole mycobacteria has been favoured in the past to diagnose tuberculosis, culturing mycobacteria is costly and microscopy is often not sensitive enough due to low bacterial loads. Detection of Mycobacterium tuberculosis biomarkers in

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26

Wang, Yan [Verfasser]. "In vitro selection and characterization of DNA aptamers against a multiple myeloma monoclonal protein / Yan Wang." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1023496372/34.

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27

Liu, Ying. "Development of ss DNA aptamers for c-Myc:Max by SELEX (Systematic Evolution of Ligands by Exponential Enrichment)." [Ames, Iowa : Iowa State University], 2007.

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28

Stephens, Matthew Jon Craig. "DNA aptamers that selectively label eukaryotic cells depending on the expression of the cell surface protein, P2X7." Thesis, University of Portsmouth, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.618284.

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The ATP gated cation channel P2X7, is a cell surface protein whose function and activation is linked with several human diseases. The aim of this project was to raise DNA Aptamers targeting specifically the extracellular domain of the P2X7 protein with the hope to use these new ligands as a method for the study of the biological functions of the P2X7 protein and its potential as a therapeutic target. The strategy involved isolating Aptamers that would bind to human em~ryonic kidney cells transformed with a construct which forced the cells to express native mouse P2X7 on its cell surface (POSIT

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29

Ric, Audrey Marie Amélie. "Caractérisation d'aptamères par électrophorèse capillaire couplée au séquençage haut-débit Illumina." Thesis, Toulouse 3, 2017. http://www.theses.fr/2017TOU30388/document.

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Les aptamères sont des oligomères d'ADN ou d'ARN simple brin qui, en se repliant sous forme de structures tridimensionnelles peuvent avoir des interactions fortes et spécifiques envers un certain nombre de cibles. L'objectif de cette thèse a été de compléter les études existantes sur l'utilisation de l'électrophorèse capillaire (CE) et les aptamères afin de mettre au point une méthode de sélection d'aptamères par CE couplée à la fluorescence induite par laser et le séquençage haut-débit Illumina. Dans un premier temps, nous avons mis au point une méthode de détection et de séparation par élect

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Orabi, Ahmed El-Sayed Abd El-Halem. "In vitro selection and characterization of single stranded DNA aptamers inhibiting the hepatitis B virus capsid-envelope interaction." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-159725.

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31

Zara, Lorena. "Développement de biocapteurs à base d'aptamères pour la détection de petites molécules Engineering Light-Up Aptamers for the Detection of RNA Hairpins through Kissing Interaction Anti-Pesticide DNA Aptamers Fail to Recognize their Targets with Asserted Micromolar Dissociation Constants A malachite green light-up aptasensor 1 for the detection of 2 theophylline." Thesis, Université Grenoble Alpes, 2020. http://www.theses.fr/2020GRALV033.

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De nos jours, il est urgent de créer des biocapteurs peu coûteux, efficaces et simples à utiliser, capables de quantifier et de détecter rapidement des petites molécules (masse molaire <1000 g / mol) telles que les pesticides, les toxines, les antibiotiques ou les médicaments, pour protéger, à la fois la santé humaine et l’environnement. Les aptamères sont des oligonucléotides en série ARN ou ADN présentant une affinité et une spécificité élevées pour leur cible. Ils peuvent être utilisés comme éléments de reconnaissance moléculaire dans des biocapteurs, appelés aptacapteurs. Ces derniers o

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Olowu, Rasaq Adewale. "Impedimetric and electrode kinetic dynamics of DNA aptamer nanobiosensors for estrogeneous endocrine disruptors." University of the Western Cape, 2011. http://hdl.handle.net/11394/8455.

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Philosophiae Doctor - PhD<br>In this work, DNA aptamer biosensor systems were developed for the detection of l7p-estradiol - an estrogeneous endocrine disrupting chemical (EDC). Endocrine disrupting chemicals are group of compounds that impact negatively on the endocrine system of humans and wildlife. High concentrations of l7p-estradiol in water or food chain disrupts the physiology of the endocrine system of various animal species, leading to feminisation in fish and stimulates the proliferation of cancer cells in humans. Aptasensor systems for the determination of l7pestradiol were prepared

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Wellhausen, Jeffrey Daniel. "Design of functional RNAs through combinatorial selections and characterization of a fluorescent cytosine analogue in DNA /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/8507.

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34

Dücker, Christina [Verfasser]. "Proteolytische Aktivität von APC bei der Inaktivierung verschiedener Faktor VIII-Präparate und bei der Faktor VIIIa-Inaktivierung in Gegenwart eines APC-DNA-Aptamers / Christina Dücker." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/1027815820/34.

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Orabi, Ahmed El-Sayed Abd El-Halem [Verfasser], and Gerd [Akademischer Betreuer] Sutter. "In vitro selection and characterization of single stranded DNA aptamers inhibiting the hepatitis B virus capsid-envelope interaction / Ahmed El-Sayed Abd El-Halem Orabi. Betreuer: Gerd Sutter." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://d-nb.info/1038152380/34.

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36

Nery, Arthur Andrade. "Purificação de células troco de lipoaspirado humano por aptâmeros de DNA, seguida da caracterização dos fenótipos obtidos da diferenciação neuronal." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-02102014-083926/.

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Células tronco mesenquimais de tecido adiposo, são uma promissora ferramenta para aplicações clínicas em terapias celular e regenerativa, em vista da facilidade de sua extração e da maior quantidade de células por unidade de massa de tecido quando comparado a outras fontes clássicas de células mesenquimais como medula óssea. O protocolo clássico de extração e purificação dessas células, depende de sua adesão em plástico e xeno-materiais demandando muito tempo para ser utilizado por médicos para auxiliar pacientes em procedimentos de emergência. Estas células são capazes se diferenciar em diver

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Cisse, Cheickna. "Etude structurale des aptamères peptidiques anti-Fur et de leur interaction avec leur cible." Phd thesis, Université de Grenoble, 2012. http://tel.archives-ouvertes.fr/tel-00846781.

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Fur (Ferric Uptake Regulator) est un régulateur transcriptionnel spécifique des bactéries qui intervient dans le contrôle de l'homéostasie du fer, ce qui en fait une cible antibactérienne intéressante. Avant mon arrivée au laboratoire, quatre inhibiteurs interagissant spécifiquement avec Fur avaient été isolés. La partie active de ces inhibiteurs consiste en des peptides de 13 acides aminés. Au cours de cette thèse, j'ai utilisé une double-approche : théorique et expérimentale pour étudier l'interaction de ces peptides avec Fur afin de comprendre le mécanisme d'inhibition. J'ai synthétisé plus

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Yangyuoru, Philip. "Investigation of the Formation of some Biologically Relevant Small Molecules Using Laser Tweezers and Capillary Electrophoresis." Kent State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=kent1406541346.

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Piccolo, Stefano. "Biophysical characterization of aptamer-ligand interactions by native mass spectrometry." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0276.

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Les aptamères sont des acides nucléiques capables de se lier sélectivement à un ligand ou à une famille de molécules. Les aptamères sont la partie sensible des riboswitches, qui sont des segments régulateurs de l'ARN messager impliqués dans l'expression génétique. Les aptamères ont aussi des applications prometteuses comme sondes artificielles et capteurs Pour ces technologies, il est crucial de comprendre comment la liaison se produit, de la quantifier, et de comprendre comment les changements conformationnels sont induits par les ligands. Les objectifs de cette thèse sont d'explorer l'applic

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Catlin, Diane M. "DNA Aptamer Confirmation and Utilization for the Cyanotoxin, Cylindrospermopsin." FIU Digital Commons, 2016. http://digitalcommons.fiu.edu/etd/2552.

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Cyanotoxins are posing an increasing threat to the health of humans and wildlife. Cylindrospermopsin is a cyanotoxin that occurs in warm climates and is harmful when ingested. The toxic effects of CYN can affect multiple organ systems. The effects, coupled with the evidence of a mass contamination of a water supply in Australia, prove that CYN needs to be investigated further. Aptamers have become a desirable method for detection of CYN as a result of an aptamer’s high specificity and the ability to scale up experiments. Aptamers have been designed to bind with a variety of targets, including

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Pla, Blasco Luis. "New nanostructured supports with signal amplification features for the detection of molecules and biomolecules of interest." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/166500.

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[ES] La presente tesis doctoral titulada "New nanostructured suports with signal amplification features for the detection of molecules and biomolecules of interest" se centra en el diseño y preparación de nuevos materiales híbridos orgánicos-inorgánicos constituidos por puertas moleculares soportadas sobre alúmina mesoporosa con el objetivo de desarrollar nuevos sistemas sensores con aplicaciones potenciales en el campo de la diagnosis y del control alimentario. En el primer capítulo de la tesis se introducen los conceptos en los que están basados los estudios realizados y los materiales prep

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42

Zhang, Haiyan. "Development of a novel, functional quantum dot-DNA/aptamer sensing technology." Thesis, University of Leeds, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658610.

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Aptamers are short single-stranded DNA or RNA oligonucleotides artificially selected against specific targets. They exhibit high target binding affinity and exquisite specificity, making them very useful in developing biosensors for a wide range of targets, such as proteins, peptides, amino acids, drugs, metal ions and even whole cells. While fluorescent semiconductor nanocrystals, also known as quantum dots (QDs), have unique size-dependent, bright and extremely photo-stable fluorescence that make them as excellent fluorescent labels for biological imaging, sensing, cell tracking/trafficking

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Berg, Katharina [Verfasser], and Ulrich [Akademischer Betreuer] Hahn. "IDA - ein Integrin-spezifisches DNA-Aptamer / Katharina Berg. Betreuer: Ulrich Hahn." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2016. http://d-nb.info/1097561682/34.

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Berg, Katharina Verfasser], and Ulrich [Akademischer Betreuer] [Hahn. "IDA - ein Integrin-spezifisches DNA-Aptamer / Katharina Berg. Betreuer: Ulrich Hahn." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2016. http://d-nb.info/1097561682/34.

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Faryammanesh, Rassa [Verfasser], and Ulrich [Akademischer Betreuer] Hahn. "Selektion und Charakterisierung Selektin-spezifischer DNA-Aptamere / Rassa Faryammanesh. Betreuer: Ulrich Hahn." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2014. http://d-nb.info/105299668X/34.

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Darnaud, Marion. "Creation of a DNA aptamer biosensor for the detection of heart failture." Thesis, University of Strathclyde, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501885.

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Ali, Shujaat. "Sélection des aptamères de l'ADN contre les biomarqueurs du mélanome." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0154.

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Le mélanome est un cancer qui représente la grande majorité de la morbidité et de la mortalité causées par tout cancer de la peau en raison de son implication dans les métastases. Parmi plusieurs biomarqueurs rapportés, les récepteurs du domaine de la discoïdine (DDR) et la métalloprotéinase Matrix (MMP) sont également considérés comme des acteurs potentiels du mélanome. Des études récentes démontrent que les DDR et les MMP sont surexprimés dans les mélanomes de mauvais pronostic, ce qui nécessite le recours à des sondes théranostiques à biomarqueurs spécifiques. De nombreux progrès ont été ré

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Mir, Llorente Mònica. "Oligonucleotide Based-Biosensors for Label-Free Electrochemical Protein and DNA Detection." Doctoral thesis, Universitat Rovira i Virgili, 2006. http://hdl.handle.net/10803/8542.

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In the last years, DNA arrays have attracted increasing attention among medical diagnosis and analytical chemists. The broad range of application that has been found for DNA arrays makes them an important analytical tool. DNA arrays are relevant for the diagnosis of genetic diseases, detection of infectious agents, study of genetic predisposition, development of a personalised medicine, detection of differential genetic expression, forensic science, drug screening, food safety and environmental monitoring.<br/>Despite the great promise of DNA arrays in health care and their success in medica

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Nadal, Polo Pedro. "Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/84036.

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Lupin has recently been added to the list of allergens requiring mandatory advisory labelling on foodstuffs sold in the European Union, and since December 2008 all products containing even trace amounts of lupin must be labelled correctly. Lupin globulins consist of two major globulins called α-conglutin (11S and “legumin-like”) and β-conglutin (7S and “vicilin-like”), and another additional two globulins, γ-conglutin and δ-conglutin, which are present in lower amounts. β-conglutin is the only conglutin currently included in the list of the International Union of Immunological Societies (IUIS)

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Peng, Chunte Sam. "Two-dimensional infrared spectroscopy of nucleic acids : application to tautomerism and DNA aptamer unfolding dynamics." Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/91113.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2014.<br>Cataloged from PDF version of thesis. Vita.<br>Includes bibliographical references.<br>The structural dynamics of nucleic acids are intimately related to their biological functions; however, our ability to study these molecular dynamics has been largely impeded by the lack of techniques that possess both high time resolution and structural sensitivity. The motivation for the work in this thesis was to develop and apply two-dimensional infrared spectroscopy (2D IR) as a new experimental tool to investigate

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