Academic literature on the topic 'DNA-based approach'

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Journal articles on the topic "DNA-based approach"

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Gullaiya, Jyoti, Naresh Kumar, and Neeharika Srivastava. "Effect of formalin fixation on DNA: A Time-Based Approach." Indian Journal of Forensic Medicine and Pathology 14, no. 4 (2021): 825–30. http://dx.doi.org/10.21088/ijfmp.0974.3383.14421.7.

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Introduction:Tissue preservation is important. From it forensic scientist may extract sufficient DNA for profiling. The most commonly used preservative found in the literature is formalin. However, it causes severe side-effects on its users as well as the environment; we need to find its replacement. Method:This study examined whether formalin could preserve soft tissues (fresh) stored at 4°C for 100 days and how DNA can be extracted from it. Qualification and Quantification of the presevred samples were done. Result:The results revealed that tissues preserved in formalin failed to generate su
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Ameyaw, Y., and I. Kyere. "Analogy-based Instructional Approach a Panacea to Students' Performance in Studying Deoxyribonucleic Acid (DNA) Concepts." International Journal of Sciences Volume 7, no. 2018-05 (2018): 7–13. https://doi.org/10.5281/zenodo.3350311.

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The purpose of this study is to investigate the use of concept map to enhance academic performance of students in DNA concepts. Quasi experimental design was used for the study. Thirty-nine (39) students from Boakye Tromo Senior High/Technical School, Duayaw Nkwanta in the Brong Ahafo Region of Ghana were purposively selected and used for the study. Molecular Concept Achievement Test (MCAT) was developed, and used for both pre-test and post-test. The reliability coefficient of MCAT was established at 0.87 using Kudder-Richardson formula 20 (KR 20). All the thirty nine (39) students were taught
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SMITH, LLOYD M., ROBERT M. CORN, ANNE E. CONDON, et al. "A Surface-Based Approach to DNA Computation." Journal of Computational Biology 5, no. 2 (1998): 255–67. http://dx.doi.org/10.1089/cmb.1998.5.255.

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Roy, Pradipta, Swati Sinha, and Debashis De. "Algorithmic Approach for DNA Based Multiplexer Design." Journal of Bioinformatics and Intelligent Control 3, no. 3 (2014): 179–85. http://dx.doi.org/10.1166/jbic.2014.1084.

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Nykypanchuk, Dmytro, Mathew M. Maye, Daniel van der Lelie, and Oleg Gang. "DNA-Based Approach for Interparticle Interaction Control." Langmuir 23, no. 11 (2007): 6305–14. http://dx.doi.org/10.1021/la0637566.

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Zhu, Enqiang, Xianhang Luo, Chanjuan Liu, and Congzhou Chen. "An Operational DNA Strand Displacement Encryption Approach." Nanomaterials 12, no. 5 (2022): 877. http://dx.doi.org/10.3390/nano12050877.

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DeoxyriboNucleic Acid (DNA) encryption is a new encryption method that appeared along with the research of DNA nanotechnology in recent years. Due to the complexity of biology in DNA nanotechnology, DNA encryption brings in an additional difficulty in deciphering and, thus, can enhance information security. As a new approach in DNA nanotechnology, DNA strand displacement has particular advantages such as being enzyme free and self-assembly. However, the existing research on DNA-strand-displacement-based encryption has mostly stayed at a theoretical or simulation stage. To this end, this paper
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Kaufmann, Beate, Or Willinger, Nanami Kikuchi, et al. "An Oligo-Library-Based Approach for Mapping DNA–DNA Triplex Interactions In Vitro." ACS Synthetic Biology 10, no. 8 (2021): 1808–20. http://dx.doi.org/10.1021/acssynbio.1c00122.

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Karakose, Mehmet, and Ugur Cigdem. "QPSO-Based Adaptive DNA Computing Algorithm." Scientific World Journal 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/160687.

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DNA (deoxyribonucleic acid) computing that is a new computation model based on DNA molecules for information storage has been increasingly used for optimization and data analysis in recent years. However, DNA computing algorithm has some limitations in terms of convergence speed, adaptability, and effectiveness. In this paper, a new approach for improvement of DNA computing is proposed. This new approach aims to perform DNA computing algorithm with adaptive parameters towards the desired goal using quantum-behaved particle swarm optimization (QPSO). Some contributions provided by the proposed
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Schön, Isa, and Koen Martens. "Phylogenetic Reconstructions of Ostracodes – A Molecular Approach." Paleontological Society Papers 9 (November 2003): 71–88. http://dx.doi.org/10.1017/s1089332600002151.

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Molecular work on ostracodes has thus far either used allozyme-based or DNA-based techniques. The application of allozyme-based methods has provided numerous data on population genetics and reproductive modes in ostracodes. With this technique, it has also been possible to construct phylogenies, although these have been restricted to distance-based methods. With the usage of DNA-based methods, a new era in ostracode research has begun. It is now possible to obtain accurate estimates for genetic diversities at very fine scales, even within individuals. The DNA-based approach is also very useful
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Gupta, Ritu, and Anchal Jain. "A New Image Encryption Algorithm based on DNA Approach." International Journal of Computer Applications 85, no. 18 (2014): 27–31. http://dx.doi.org/10.5120/14942-3440.

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Dissertations / Theses on the topic "DNA-based approach"

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Wong, Winston. "DNA logic-A novel approach to semiconductor based genetics." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/6407.

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In the coming years, genetic test results will be increasingly used as indicators that influence medical decision-making. With chronic disease on the rise and the continuing global spread of infectious disease, novel instruments able to detect relevant mutations in a point-of-care setting are being developed to facilitate this increased demand in personalized health care. However, diagnosis for such demand often requires laboratory facilities and skilled personnel, meaning that diagnostic tests are restricted by time and access. This thesis presents a novel configuration for Ion sensitive Fiel
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Cangiano, daniela. "Detection of KCNJ5 mutations in the APA tissues and cell-free DNA with a novel Taqman-based approach." Doctoral thesis, Università degli studi di Padova, 2015. http://hdl.handle.net/11577/3424711.

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Background: Primary aldosteronism (PA) is the most common endocrine form of secondary arterial hypertension with an estimated prevalence of ~ 11.2% in patients referred to specialized centers and 4% in primary care, but as high as 50% in patients with resistant hypertension. The two main forms of PA are: aldosterone-producing adenoma (APA), surgically curable with the removal of adenoma that is able to overproduce aldosterone, and bilateral adrenocortical hyperplasia (BAH), which needs drug therapy. About ~ 30% APAs have somatic mutations in KCNJ5 gene that encodes for the potassium channel (K
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Nell, Erica-Mari. "A pathway-based approach investigating DNA sequence variants to implicate the inflammatory pathway in the predisposition to Achilles tendinopathy." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10807.

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Includes abstract.<br>Includes bibliographical references (leaves 159-179).<br>The aims of this dissertation were therefore (i) to follow a pathway-based approach investigating genes encoding proteins involved in the ECM degradation and apoptosis signalling cascade for associations with AT and (ii) to identify a polygenic risk model, comprised of several genetic markers within genes encoding proteins involved in the inflammatory pathway, to predict risk of AT.
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Untiveros, Katherine Lozano. "Electrochemical approach and development of an eletrochemical biosensor based on hairpin-DNA modified gold electrode for detection of DNA damage for a new acridine-thiophene cancer drug." Universidade Federal de Alagoas, 2017. http://www.repositorio.ufal.br/handle/riufal/2120.

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The interaction of drugs with DNA is a significant feature in pharmacology and plays a vital role in the designing of more efficient and specifically targeted drugs.The concept of hybridization of two bioactive molecules often leads to increased activity due to synergistic effects of anticancer drugs have been studied.Two important pharmacophores: Acridine and thiophene were widely studied as antitumor, antiparasitic and antibacterial agents. We hypothesize that a conjugate comprised two pharmacophores with different mechanisms of antiproliferative action can result in enhanced DNA damage. El
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Rykalina, Vera N. [Verfasser]. "Accurate assessment of TN5-based DNA fragmentation efficiency and evaluation of tagmentation sites indexing approach for contiguity-preserving sequencing / Vera N. Rykalina." Berlin : Freie Universität Berlin, 2017. http://d-nb.info/1137206713/34.

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Fusaro, Jonathan L. "Estimating Baseline Population Parameters of Urban and Wildland Black Bear Populations Using a DNA-Based Capture -Mark-Recapture Approach in Mono County, California." DigitalCommons@USU, 2014. https://digitalcommons.usu.edu/etd/3706.

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Prior to European settlement, black bear (Ursus americanus) were far less abundant in the state of California. Estimates from statewide harvest data indicate the California black bear population has tripled in the last 3 decades. Bears inhabit areas they formally never occurred (e.g., urban environments) and populations that were at historically low densities are now at high densities. Though harvest data are useful and widely used as an index for black bear population size and population demographics statewide, it lacks the ability to produce precise estimates of abundance and density at loca
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Reichart, Thomas. "DNA microarray based gene expression profiling in human hepatocyte cells to serve as a basis for dynamic modelling of the human liver a systems biology approach /." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-35867.

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Lehm, Manuel [Verfasser], and Martin [Akademischer Betreuer] Dichgans. "From phenotype to function via mass spec-based proteomics : an LC-MS/MS DNA-protein pulldown approach applied to functional stroke genetics / Manuel Lehm ; Betreuer: Martin Dichgans." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1202011497/34.

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Pendleton, Carly R. "A simulation-based approach for evaluating gene expression analyses /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd1753.pdf.

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CORTIMIGLIA, CLAUDIA. "DNA-BASED APPROACHES FOR MICROBIAL FOOD SAFETY AND QUALITY." Doctoral thesis, Università Cattolica del Sacro Cuore, 2022. http://hdl.handle.net/10280/120587.

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La contaminazione degli alimenti rappresenta un interesse primario per i consumatori e per produttori coinvolti nella filiera alimentare. Il monitoraggio continuo degli alimenti è quindi fondamentale per prevenire non solo la proliferazione di agenti patogeni, ma anche i microrganismi in grado di produrre un deterioramento influenzando la qualità dei prodotti finali. Da questo punto di vista, l'obiettivo della tesi è di aumentare le conoscenze sulla rilevazione e la caratterizzazione dei batteri di interesse alimentare, utilizzando metodi basati sul DNA, in particolare l'analisi del genoma, e
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Books on the topic "DNA-based approach"

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Brinkman, Todd J. Assessing population trends of deer in southeast Alaska using a DNA-based approach: A general guide, version 1.0. Alaska Dept. of Fish and Game, Division of Wildlife Conservation, 2010.

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Straalen, Nico, and Dick Roelofs. Human Evolution and Development. Amsterdam University Press, 2019. http://dx.doi.org/10.5117/9789463729208.

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Our understanding of human evolution is proceeding at an unprecedented rate over the last years due to spectacular fossil finds, reconstructions based on genome comparison, ancient DNA sequencing and new insights into developmental genetics. This book takes an integrative approach in which the development of the human embryo, the evolutionary history of our body, the structure of human populations, their dispersal over the world and their cultures are examined by integrating paleoanthropology, developmental biology, comparative zoology, population genetics and phylogenetic reconstruction. The
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Denver, Dee. The Dharma in DNA. Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780197604588.001.0001.

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The Dharma in DNA has three objectives: (1) to share the rich but underappreciated history of biology–Buddhism intersections and surprising harmonies between the two traditions, (2) to evaluate Buddhist teachings from a scientific perspective using DNA as the focus of study, and (3) to propose a new approach to science, Bodhi science, as an ethical and operational framework for conducting Buddhist wisdom–guided science and preventing pseudoscience. An interwoven side project examines the life journey of the author, a professor of genetics and father in a transracial adoptive family, who questi
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Emmert-Streib, Frank, and Matthias Dehmer. Analysis of Microarray Data: A Network-Based Approach. Wiley & Sons, Incorporated, John, 2008.

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(Editor), Matthias Dehmer, and Frank Emmert-Streib (Editor), eds. Analysis of Microarray Data: A Network-Based Approach. Wiley-VCH, 2008.

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Emmert-Streib, Frank, and Matthias Dehmer. Analysis of Microarray Data: A Network-Based Approach. Wiley & Sons, Limited, John, 2008.

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Evolution: Possible or impossible? : Molecular biology and the laws of chance in nontechnical language : a new approach to the subject, based on exciting recent discoveries involving proteins and DNA, the "golden molecule" of heredity. Probability Research in Molecular Biology, 2002.

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Coleman, Andrew. Microscopy-based approaches to the use of radiation-induced nuclear foci as biomarkers of DNA repair. 2006.

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Bensimon, David, Vincent Croquette, Jean-François Allemand, Xavier Michalet, and Terence Strick. Single-Molecule Studies of Nucleic Acids and Their Proteins. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198530923.001.0001.

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This book presents a comprehensive overview of the foundations of single-molecule studies, based on manipulation of the molecules and observation of these with fluorescent probes. It first discusses the forces present at the single-molecule scale, the methods to manipulate them, and their pros and cons. It goes on to present an introduction to single-molecule fluorescent studies based on a quantum description of absorption and emission of radiation due to Einstein. Various considerations in the study of single molecules are introduced (including signal to noise, non-radiative decay, triplet st
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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Environmental DNA. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.001.0001.

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Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a par
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Book chapters on the topic "DNA-based approach"

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Hartemink, Alexander, Tarjei Mikkelsen, and David Gifford. "Simulating biological reactions: A modular approach." In DNA Based Computers V. American Mathematical Society, 2000. http://dx.doi.org/10.1090/dimacs/054/10.

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Liu, Qinghua, Zhen Guo, Zhengdong Fei, et al. "A surface-based approach to DNA computation." In DNA Based Computers II. American Mathematical Society, 1998. http://dx.doi.org/10.1090/dimacs/044/10.

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Chandran, Harish, Nikhil Gopalkrishnan, Bernard Yurke, and John Reif. "Meta-DNA: A DNA-Based Approach to Synthetic Biology." In A Systems Theoretic Approach to Systems and Synthetic Biology II: Analysis and Design of Cellular Systems. Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9047-5_7.

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Majumdar, Abhishek, Tanusree Podder, Atanu Majumder, Nirmalya Kar, and Meenakshi Sharma. "DNA-Based Cryptographic Approach Toward Information Security." In Advances in Intelligent Systems and Computing. Springer India, 2014. http://dx.doi.org/10.1007/978-81-322-2012-1_22.

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Costa, Diana, Artur J. M. Valente, and João Queiroz. "DNA-Based Hydrogels: An Approach for Multifunctional Bioapplications." In Gels Horizons: From Science to Smart Materials. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6077-9_13.

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Ekta and Ajit Singh. "A Key Based Spiral Approach for DNA Cryptography." In Advances in Intelligent Systems and Computing. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8536-9_22.

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Kobayashi, Hiroko, Anthony A. Horner, Elena Martin-Orozco, and Eyal Raz. "Pre-priming: a novel approach to DNA-based vaccination and immunomodulation." In Immunostimulatory DNA Sequences. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-56866-4_9.

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Ramampiaro, Heri, and Aleksander Grande. "DNA Sequence Search Using Content-Based Image Search Approach." In Advances in Intelligent and Soft Computing. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-19914-1_26.

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Romano, Flavio, and Lorenzo Rovigatti. "A Nucleotide-Level Computational Approach to DNA-Based Materials." In Design of Self-Assembling Materials. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-71578-0_3.

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Verma, Kartik, Butta Singh, Satveer Kour, et al. "Chaotic Maps and DNA Encoding-Based Image Encryption Approach." In Lecture Notes in Electrical Engineering. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-4391-2_11.

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Conference papers on the topic "DNA-based approach"

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Sreeram, Anusha, and Raju Bhukya. "Enhancing DNA Sequence Analysis for Disease Diagnosis and Prediction Using Parallelised Hash-Based Approach." In 2025 2nd International Conference on Computational Intelligence, Communication Technology and Networking (CICTN). IEEE, 2025. https://doi.org/10.1109/cictn64563.2025.10932374.

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Shaltout, Nawal, Ahmed A. Abd El-Latif, Waleed Al-Adrousy, Mohamed Hammad, Abdelhamied A. Ateya, and Samir Elmougy. "A Novel Image Encryption and Decoding Approach Based on DNA Coding and Arnold's Transformation for IoT Applications." In 2024 International Telecommunications Conference (ITC-Egypt). IEEE, 2024. http://dx.doi.org/10.1109/itc-egypt61547.2024.10620507.

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Croese, Elsemiek, Paul Hennessey, Herman de Vries, et al. "Oil & Gas Water Injection Treatment System Case Study: Field Trial Comparing Biocide/UV Disinfection and New Monitoring Techniques for Mitigating MIC and Reservoir Souring." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09114.

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Abstract Microbial Influenced Corrosion (MIC) is difficult to control. Therefore, biocides are often added as a preventive measure to exterminate microbial activity and thereby also mitigate MIC. Biocides have the disadvantage that they have to be added continuously and can be hazardous to employees and the environment. UV treatment provides an alternative to biocide, but the effectiveness of UV treatment compared to biocide treatment has to be proven before implementation in a specific system. The effectiveness of biocide treatment is often measured using culturing methods, however, one of th
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Jack, T. R., G. Voordouw, A. Agrawal, J. K. Voordouw, and T. Place. "Microbial Communities Associated with External Corrosion on an Operating Pipeline System." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-3911.

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Abstract Emerging molecular methods based on the analysis of DNA extracted from field samples provide a new opportunity to characterize microbial populations associated with corrosion problems in the oil and gas industry. This paper reports the application of these methods to samples obtained from the excavation of external corrosion sites under disbonded coating on an operating transmission pipeline. Insights into the nature of the microbial communities associated with corrosion products, trapped water, coating and soil will be presented along with an assessment of the potential of this appro
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Larsen, Jan, Ketil Sørensen, Bo Højris, and Torben Lund Skovhus. "Significance of Troublesome Sulfate-Reducing Prokaryotes (SRP) in Oil Field Systems." In CORROSION 2009. NACE International, 2009. https://doi.org/10.5006/c2009-09389.

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Abstract Sulfide generation by sulfate-reducing prokaryotes (SRP) is the major cause of reservoir souring and microbiologically influenced corrosion (MIC). The monitoring of SRP in oil fields is typically carried out by cultivation based methods. It is widely accepted that the cultivation approach grossly underestimates population sizes by several orders of magnitude due to the majority of SRP in oil field samples being not readily viable in selective culture media. In this paper molecular microbiology methods (MMM) are used to unveil the true distribution of SRP in oil field systems. These te
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Subasini, C. A., Sarvesh N. R, and Vasanthakumar D. "Advancing Precision Medicine: Machine Learning Approaches for DNA-based Disease Identification." In 2025 International Conference on Intelligent Computing and Control Systems (ICICCS). IEEE, 2025. https://doi.org/10.1109/iciccs65191.2025.10985020.

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Geurkink, Bert, Sabine Doddema, Herman de Vries, Gert Jan Euverink, and Elsemiek Croese. "Value of Next Generation Sequencing as Monitoring Tool for Microbial Corrosion a Practical Case from Bioprophyling to Tailor Made MMM Analysis." In CORROSION 2016. NACE International, 2016. https://doi.org/10.5006/c2016-07764.

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Abstract Microbiologically Influenced Corrosion (MIC) can occur unexpectedly in any kind of system in which metal surfaces are involved, leading to integrity problems and economic loss. To manage and control MIC we need a clearer understanding of the microbial communities attached to the metal surfaces and how they influence the chemical processes involved in corrosion. There are several methods (e.g. biocide dosing, coating of materials, removing water) for controlling MIC, but the effectiveness of these methods depends on the type of microorganisms present and the prevailing conditions. A th
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Chung, C. L., K. Nguyen, S. Lyonnais, et al. "DNA Linked To Single Wall Carbon Nanotubes: Covalent Versus Non-Covalent Approach." In DNA-BASED NANODEVICES: International Symposium on DNA-Based Nanodevices. AIP, 2008. http://dx.doi.org/10.1063/1.3012293.

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Wolff, A., W. Fritzsche, M. Kittler, et al. "A Novel Approach To Self-organized Pattern Formation Of Biomolecules At Silicon Surfaces." In DNA-BASED NANOSCALE INTEGRATION: International Symposium on DNA-Based Nanoscale Integration. AIP, 2006. http://dx.doi.org/10.1063/1.2360584.

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Beegom, S. Bismi, and Sangeetha Jose. "An enhanced cryptographic model based on DNA approach." In 2017 International Conference of Electronics, Communication and Aerospace Technology (ICECA). IEEE, 2017. http://dx.doi.org/10.1109/iceca.2017.8212824.

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Reports on the topic "DNA-based approach"

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Weil, Clifford F., Anne B. Britt, and Avraham Levy. Nonhomologous DNA End-Joining in Plants: Genes and Mechanisms. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585194.bard.

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Repair of DNA breaks is an essential function in plant cells as well as a crucial step in addition of modified DNA to plant cells. In addition, our inability to introduce modified DNA to its appropriate locus in the plant genome remains an important hurdle in genetically engineering crop species.We have taken a combined forward and reverse genetics approach to examining DNA double strand break repair in plants, focusing primarily on nonhomologous DNA end-joining. The forward approach utilizes a gamma-plantlet assay (miniature plants that are metabolically active but do not undergo cell divisio
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Seale, Maria, Natàlia Garcia-Reyero, R. Salter, and Alicia Ruvinsky. An epigenetic modeling approach for adaptive prognostics of engineered systems. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41282.

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Prognostics and health management (PHM) frameworks are widely used in engineered systems, such as manufacturing equipment, aircraft, and vehicles, to improve reliability, maintainability, and safety. Prognostic information for impending failures and remaining useful life is essential to inform decision-making by enabling cost versus risk estimates of maintenance actions. These estimates are generally provided by physics-based or data-driven models developed on historical information. Although current models provide some predictive capabilities, the ability to represent individualized dynamic f
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Paterson, Andrew H., Yehoshua Saranga, and Dan Yakir. Improving Productivity of Cotton (Gossypsum spp.) in Arid Region Agriculture: An Integrated Physiological/Genetic Approach. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7573066.bard.

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Objectives: We seek to establish the basis for improving cotton productivity under arid conditions, by studying the water use efficiency - evaporative cooling interrelationship. Specifically, we will test the hypothesis that cotton productivity under arid conditions can be improved by combining high seasonal WUE with efficient evaporative cooling, evaluate whether high WUE and/or evaporative cooling are based on specific physiological factors such as diurnal flexibility in stomatal conductance, stomatal density, photosynthetic capacity, chlorophyll fluorescence, and plant water status. Genes i
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Palmer, Guy, Varda Shkap, Wendy Brown, and Thea Molad. Control of bovine anaplasmosis: cytokine enhancement of vaccine efficacy. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7695879.bard.

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Anaplasmosis an arthropod-born disease of cattle caused by the rickettsia Anaplasma marginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently development of a safe, effective vaccine is a high priority. In this collaborative project we focused
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Burns, Malcom, and Gavin Nixon. Literature review on analytical methods for the detection of precision bred products. Food Standards Agency, 2023. http://dx.doi.org/10.46756/sci.fsa.ney927.

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The Genetic Technology (Precision Breeding) Act (England) aims to develop a science-based process for the regulation and authorisation of precision bred organisms (PBOs). PBOs are created by genetic technologies but exhibit changes which could have occurred through traditional processes. This current review, commissioned by the Food Standards Agency (FSA), aims to clarify existing terminologies, explore viable methods for the detection, identification, and quantification of products of precision breeding techniques, address and identify potential solutions to the analytical challenges presente
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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate
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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of
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Joel, Daniel M., Steven J. Knapp, and Yaakov Tadmor. Genomic Approaches for Understanding Virulence and Resistance in the Sunflower-Orobanche Host-Parasite Interaction. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592655.bard.

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Oroginal Objectives: (i) identify DNA markers linked to the avirulence (Avr) locus and locate the Avr locus through genetic mapping with an inter-race Orobanche cumana population; (ii) develop high-throughput fingerprint DNA markers for genotypingO. cumana races; (iii) identify nucleotide binding domain leucine rich repeat (NB-LRR) genes encoding R proteins conferring resistance to O. cumana in sunflower; (iv) increase the resolution of the chromosomal segment harboring Or₅ and related R genes through genetic and physical mapping in previously and newly developed mapping populations of sunflow
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Hedrick, Ronald, and Herve Bercovier. Characterization and Control of KHV, A New Herpes Viral Pathogen of Koi and Common Carp. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7695871.bard.

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In this project we proposed to characterize the virus genome and the structural virion polypeptides to allow development of improved diagnostic approaches and potential vaccination strategies. These goals have been mostly achieved and the corresponding data were published in three papers (see below) and three more manuscripts are in preparation. The virion polypeptides of KHV strains isolated from USA (KHV-U) and Israel (KHV-I) were found to be identical. Purified viral DNA analyzed with a total of 5 restriction enzymes demonstrated no fragment length polymorphism between KHV-I and KHV-U but b
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Katz, Aron, Mark Johnson, and Jinelle Sperry. Environmental DNA sampling for at-risk and invasive species management on military ranges : guidelines and protocols for installation biologists and land managers. Engineer Research and Development Center (U.S.), 2024. http://dx.doi.org/10.21079/11681/48275.

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Environmental DNA (eDNA) analysis, or the detection of trace DNA shed by organisms into their environment, has the potential to transform Army capabilities for threatened and endangered species (TES) and invasive species management by providing a rapid, noninvasive, and cost-effective option for monitoring wildlife. Despite these benefits, eDNA analysis is underutilized on military installations as limited access to guidance materials, protocols, training opportunities, and support from eDNA scientists makes it difficult for installation biologists and military land managers to design and exec
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