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Dissertations / Theses on the topic 'DNA-damaged'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Upton, Amy Louise. "Replication of damaged DNA." Thesis, University of Nottingham, 2009. http://eprints.nottingham.ac.uk/11332/.

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DNA is under constant attack from numerous damaging agents and our cells deal with thousands of lesions every day. With such constant damage it is inevitable that the template will not be completely cleared of lesions before the replication complex arrives. The consequences of the replisome meeting an obstacle will depend upon the nature of the obstacle. I have focussed upon replication in Escherichia coli and the effect of UV-induced lesions, which would block synthesis by the replicative polymerases. It is accepted that a UV lesion in the lagging strand template can be bypassed by the replis

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2

Brown, Stephanie Marie. "Replication of damaged DNA in mammalian cells." Thesis, University of Sussex, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445620.

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Turner, Jayne Susan. "Molecular recognition of damaged DNA using synthetic affinity reagents." Thesis, Open University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418471.

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Potentially carcinogenic DNA damage that may be derived from dietary sources can be quantified using a number of methods. Some of the best of these methods are based on the use of antibodies that have high affinity and specificity for modified DNA bases. However, given the sometimes limited availability of antibodies there is a requirement for novel reagents that mimic the properties of the best antibodies. From a chemical perspective antibodies are `over engineered' and synthetic affinity reagents would recreate the optimal properties of the binding site with respect to selectivity and affini

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4

Zamble, Deborah B. (Deborah Beth) 1971. "The responses of cellular proteins to cisplatin-damaged DNA." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/73344.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1999.<br>Vita.<br>Includes bibliographical references.<br>The anticancer drug cisplatin is a small inorganic molecule that forms several types of covalent adducts on DNA. There is evidence that at least some of the consequences of this genetic damage are mediated by proteins that bind to the cisplatin-DNA cross-links or influence cellular pathways in response to the genotoxic stress. In either case, such factors can regulate the processing of the cisplatin lesions and thereby affect cellular sensitivity to the drug. Ide

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Jung, Yongwon 1977. "Cellular responses against DNA damaged by platinum anticancer drugs." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/33746.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.<br>Vita.<br>Includes bibliographical references.<br>The anticancer activity of platinum-based drugs such as cisplatin, carboplatin, and oxaliplatin is mediated by their ability to attack DNA such that generated adducts trigger numerous cellular responses. A better understanding of these processes is critical for developing more effective therapeutic approaches, which can increase the anti-cancer activity of the drugs while minimizing side effects and extending successful treatment to a wider range of human cancer

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6

Baynton, Kathy J. "Replication of damaged dna in the yeast saccharomyces cerevisiae." Université Louis Pasteur (Strasbourg) (1971-2008), 1998. http://www.theses.fr/1998STR13123.

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Les adduits formes par le cancerogene n-2-acetylaminofluorene (aaf) sur le c8 de la guanine sont tres bloquants lors de la replication et induisent principalement des mutations par decalage du cadre du lecture. Un systeme a ete developpe permettant de mesurer le niveau de synthese en face d'une lesion aaf (stl), une des strategies de tolerance des lesions dans la levure saccharomyces cerevisiae. Des vecteurs heteroduplexes contenant sur un brin la sequence cible (5'-ggg#a#a#f-3' ou 5'-ggcg#a#a#fcc-3') et sur le brin complementaire une heterologie de sequence constituee d'une boucle de 4 nucleo

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7

Hatton, David H. "DNA repair and replication of ultraviolet-damaged human melanoma cell lines." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386324.

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8

Zainol, Murizal. "Assessment of phytochemicals in preventing oxidatively damaged DNA in bladder cancer." Thesis, University of Leicester, 2011. http://hdl.handle.net/2381/10158.

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Oxidatively damaged DNA is thought to be important in both the initiation and development of bladder carcinoma. Phytochemical compounds are thought to promote optimal health, partly via their antioxidant effects in protecting cellular components against damaging free radicals. The objective of this study is to assess the effect of a standardised bilberry extract, mirtoselect, on the level of endogenous and induced oxidatively damaged DNA in bladder cancer cells, as assessed by the Comet assay. The Comet assay, also known as single-cell gel electrophoresis, represents a simple method for measur

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9

Hall, Ashley. "ASSESSMENT AND IN VITRO REPAIR OF DAMAGED DNA TEMPLATES FROM FORENSIC STAINS." Doctoral diss., University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2621.

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DNA extracted from biological stains is often intractable to analysis. This may due to a number of factors including a low copy number (LCN) of starting molecules, the presence of soluble inhibitors or damaged DNA templates. Remedies may be available to the forensic scientist to deal with LCN templates and soluble inhibitors but none presently exist for damaged DNA. In fact, only recently has the biochemical nature, the extent of DNA damage in physiological stains and the point at which the damage inflicted upon a particular sample precludes the ability to obtain a genetic profile for purposes

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10

Hurley, Eldon Kenneth Jr. "Photolyase: Its Damaged DNA Substrate and Amino Acid Radical Formation During Photorepair." Thesis, Virginia Tech, 2005. http://hdl.handle.net/10919/31084.

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Ultraviolet light damages genomic material by inducing the formation of covalent bonds between adjacent pyrimidines. Cis-syn cyclobutane pyrimidine dimers (CPD)constitute the most abundant primary lesion in DNA. Photolyase, a light-activated enzyme, catalytically repairs these lesions. Although many steps in the photolyase-mediated repair process have been mapped, details of the mechanism remain cryptic. Difference FT-IR spectroscopy was employed to obtain new mechanistic information about photorepair. Purified oligonucleotides, containing a central diuracil, dithymidine, or cyclobutane thymid

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11

Frey, Alexander. "The role of histone variant H3.3 in the replication of damaged DNA." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708717.

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12

MICLOT, Tom. "Modeling the influence of DNA lesion on the regulation of gene expression." Doctoral thesis, Università degli Studi di Palermo, 2022. https://hdl.handle.net/10447/573632.

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Nucleic acids are organic macromolecules that result from the polymerization of nucleotides. These molecules are generally considered as the support of the genetic information. Two families of nucleic acids are currently known: DNA and RNA. From a structural point of view, the most popular form is the double helix of DNA. However, other forms exist and among them are the G-quadruplex. This is a folding of the DNA, or RNA, in an area rich in guanines. These form quadruplex of guanines, which are stacked on top of each other and are stabilized by a central cation. G-quadruplex structures

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13

Godfrey, D. B. "Cloning of a gene involved in replication of DNA on a damaged template." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259596.

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14

Protopopova, Marina. "Modulation of activity of the tumour suppressor p53 by small molecules and damaged DNA /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-926-9/.

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15

Pryor, John Michael. "Studies of the Rev1 protein and its role in the replication of damaged DNA." Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/5044.

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Sites of unrepaired DNA damage block DNA synthesis by replicative polymerases and interrupt replication fork progression. This is a problem because stalled replication forks can collapse leading to DNA double-stranded breaks, chromosome instability, and eventually cell death. The translesion DNA synthesis (TLS) pathway rescues stalled replication forks by using polymerases that are specialized for replicating damaged DNA, called non-classical polymerases. The use of TLS is risky however, because it can result in replication errors leading to mutations and eventually carcinogenesis. Genetic stu

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16

Oksenych, Valentyn. "Molecular mechanisms of recruitment of transcription/repair factor TFIIH to the sites of damaged DNA." Strasbourg, 2009. https://publication-theses.unistra.fr/public/theses_doctorat/2009/OKSENYCH_Valentyn_2009.pdf.

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Bafail, Duaa. "The Effect of Damaged Bases on The End Joining of DNA Double Strand Break Ends." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3516.

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DNA double strand breaks (DSBs ) are extremely toxic to cells because they can lead to genomic rearrangements and even cell death. Two main pathways can repair DSBs: the homologous recombination repair (HRR) pathway and the non-homologous end-joining (NHEJ) pathway. NHEJ is the primary repair pathway in mammalian cells. HRR repairs single strand breaks (SSBs) or DSBs, mostly during late S phase and G2 phase of the cell cycle, by using an undamaged copy of the DNA sequence, and is therefore largely error-free, while the NHEJ pathway repairs DSBs without the requirement for sequence homology, ma

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Roth, Heide Marie [Verfasser], and Caroline [Akademischer Betreuer] Kisker. "Nucleotide Excision Repair : From Recognition to Incision of damaged DNA / Heide Marie Roth. Betreuer: Caroline Kisker." Würzburg : Universitätsbibliothek der Universität Würzburg, 2012. http://d-nb.info/1021645842/34.

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19

Abdullah, Ummi. "Biochemical studies on the role of the XPF-ERCC1 endonuclease in repairing damaged DNA replication forks." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:e42b78ee-667a-4545-9ddd-27be5aea99b4.

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The human XPF (ERCC4) and ERCC1 proteins form a heterodimeric endonuclease that plays a critical role in maintaining genome stability. Mutations in the XPF gene cause several heritable disorders including Fanconi anemia (FA). This highlights the importance of DNA repair processes controlled by XPF-ERCC1, including the repair at replication forks stalled by DNA interstrand crosslinks (ICLs), for human health. Unrepaired ICLs, formed by by-products of cellular metabolism, are believed to be responsible for the genomic instability, cellular attrition and cancer predisposition seen in FA patients.

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20

Treiber, Daniel Kelly 1965. "The role of damaged DNA recognition proteins in the genotoxicities of ultraviolet light and the anticancer drug cisplatin." Thesis, Massachusetts Institute of Technology, 1993. http://hdl.handle.net/1721.1/37508.

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21

Klotz, Rémi. "Rôle de la protéine Damaged DNA Binding 2 dans la réponse des cellules tumorales mammaires aux agents thérapeutiques." Thesis, Université de Lorraine, 2014. http://www.theses.fr/2014LORR0134/document.

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Le laboratoire a récemment identifié la protéine Damaged-DNA-Binding 2 (DDB2), connue à l’origine pour son rôle dans la réparation de l’ADN comme une protéine impliquée dans la tumorigenèse mammaire. En effet, nous avons montré son rôle dans la croissance et la progression des tumeurs mammaires via la régulation transcriptionnelle de gènes cibles. Dans ce travail, nous avons montré que la surexpression de DDB2 augmente la sensibilité des cellules tumorales MDA-MB231 et SKBr3 traitées à la doxorubicine et au 5-fluorouracile (5-FU). Inversement, l’inhibition de l’expression de DDB2 dans les cell

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22

Kattan, Zilal. "Rôle de la superoxyde dismutase à manganèse et de la protéine damaged DNA binding 2 dans la croissance tumorale mammaire." Thesis, Nancy 1, 2009. http://www.theses.fr/2009NAN10040/document.

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Récemment, notre laboratoire a démontré pour la première fois, que la protéine Damaged DNA Binding 2 (DDB2) possédait une activité régulatrice négative de l’expression basale de la superoxyde dismutase mitochondriale (SOD Mn) en se fixant sur un élément de réponse dans la région promotrice de son gène. Cette protéine était connue jusque là pour sa participation dans le système de réparation de l’ADN par excision de nucléotides. L’objectif de ce travail a été de définir précisément l’implication de ces deux protéines dans la croissance des cellules d’adénocarcinome mammaire, en développant des

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23

Singatulina, Anastasiya. "The link between PARP1-mediated signaling and RNA-binding protein FUS in response to DNA damage PARP-1 Activation Directs FUS to DNA Damage Sites to Form PARG-Reversible Compartments Enriched in Damaged DNA." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLE042.

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La stabilité du génome est contrôlée par une machinerie complexe qui répare les dommages causés à l'ADN, le principal responsable du cancer et des maladies associées au vieillissement. En effet, Les cellules humaines sont exposées à différentes sources de stress oxydatif qui entraînent des dommages de l'ADN, notamment les cassures simple brin et les cassures double brin. Les sources de cassures de l’ADN peuvent être exogènes (lumière ultraviolette, pollution, rayonnements ionisants) ou endogènes (métabolisme cellulaire et inflammation). Après un stress génotoxique, les cellules de mammifères d

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Kattan, Zilal Becuwe Philippe. "Rôle de la superoxyde dismutase à manganèse et de la protéine damaged DNA binding 2 dans la croissance tumorale mammaire." S. l. : Nancy 1, 2009. http://www.scd.uhp-nancy.fr/docnum/SCD_T_2009_0040_KATTAN.pdf.

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25

Bignon, Emmanuelle. "Theoretical approach of complex DNA lesions : from formation to repair." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1085/document.

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Ce travail de thèse vise à étudier l'endommagement de l'ADN, de la formation de lésions à leur réparation par des méthodes de modélisation moléculaire. Plusieurs projets ont pris forme dans ce contexte, lesquels peuvent être classés en trois grandes catégories. D'un côté, nous nous sommes intéressés la formation de lésions induites par des agents mutagènes. Nous avons étudié les mécanismes de formation de la 8-oxo-7,8-dihydroguanine (8oxoG), mais aussi le caractère de photosensibilisateur endogène de la pyrimidine 6-4 pyrimidone (6-4PP), et la photosensibilisation de l'ADN par deux anti-inflam

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Lourdin, Morgane. "Etudes biochimiques et structurales de la réparation des lésions multiples de l'ADN." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENV007/document.

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27

Barbieux, Claire. "Rôles de la protéine Damaged-DNA Binding 2 sur l’adhérence, les propriétés nanomécaniques et la voie du TGFß1 dans les cellules tumorales mammaires." Thesis, Université de Lorraine, 2015. http://www.theses.fr/2015LORR0337/document.

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La compréhension des mécanismes à l’origine de la progression métastatique des tumeurs mammaires reste une préoccupation constante en cancérologie et nécessite la découverte de nouveaux marqueurs prédictifs. Dans ce sens, le laboratoire a montré que la protéine DDB2 (Damaged-DNA Binding 2) était impliquée dans la tumorigenèse mammaire, en favorisant la prolifération et en réduisant le pouvoir invasif des cellules tumorales. Les propriétés invasives des cellules étant étroitement liées à leurs capacités d’adhérence, nous nous sommes intéressés au rôle de DDB2 dans l’adhérence des cellules tumor

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Ganpudi, Ashwin. "The role of Arabidopsis Damaged DNA Binding Protein 1B (DDB1B) and genetic interactions with DDB1A, DDB2, De-etiolated1 (DET1) and Constitutive Photomorphogenic 1 (COP1)." Intech, 2011. http://hdl.handle.net/1993/4994.

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Damaged DNA Binding Protein 1 (DDB1) – CULLIN4 E3 ubiquitin ligase complexes have been implicated in a variety of biological processes in a range of organisms. Uniquely, Arabidopsis thaliana encodes two homologs of DDB1, DDB1A and DDB1B. In this study we utilize a viable partial loss of function allele of DDB1B, ddb1b-2, to examine genetic interactions with DDB1A, DET1 and COP1. While the ddb1b-2 ddb1a double mutant is lethal, ddb1a ddb1b-2/+ and ddb1b-2 ddb1a/+ heterozygotes do not exhibit any developmental phenotypes. These heterozygotes do however exhibit decreased UV tolerance. In addition

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Minig, Vanessa. "Etude du mécanisme de régulation du gène et de l'importance biologique de la superoxyde dismutase à manganèse dans la croissance tumorale mammaire." Thesis, Nancy 1, 2009. http://www.theses.fr/2009NAN10032/document.

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La superoxyde dismutase à Manganèse (SOD Mn ou SOD2) est une enzyme importante dans la défense antioxydante, qui semble jouer un rôle mal défini dans le développement des tumeurs selon l’expression constitutive de son gène. Cependant, les mécanismes de régulation de cette expression constitutive sont mal connus, en particulier dans les cellules tumorales mammaires. Ce travail a reposé sur la mise en évidence préalable d’une protéine, appelée la Damaged DNA Binding 2 (DDB2) protein, se fixant spécifiquement sur la région promotrice du gène SOD2. La DDB2 est connue pour sa participation dans la

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Ennen, Marie. "Mise en évidence d'une relation entre la protéine Damaged DNA-Binding 2 et le facteur de transcription NF-kB : conséquences sur les capacités migratrices et invasives des tumeurs mammaires." Phd thesis, Université de Lorraine, 2012. http://tel.archives-ouvertes.fr/tel-00878985.

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La protéine Damaged DNA-Binding 2 (DDB2) est connue pour son rôle dans la réparation de l'ADN lésé par les UV. Cependant, le laboratoire a montré que cette protéine est surexprimée naturellement dans les cellules tumorales mammaires non métastatiques et active leur prolifération, en favorisant leur entrée en phase de transition G1/S du cycle cellulaire. Il a été montré que cette nouvelle activité biologique de DDB2 dépend de sa capacité à intervenir dans la transcription de gènes cibles, comme celui codant l'enzyme anti-oxydante, la superoxyde dismutase à manganèse (SOD Mn). Sur la base que DD

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Drouot, Guillaume. "Recherche de partenaires potentiels de la protéine Damaged-DNA Binding 2 dans la régulation de l’expression génique : le cas des heterogeneous ribonucleoprotein K et J dans la régulation du gène NFKBIA." Thesis, Université de Lorraine, 2018. http://www.theses.fr/2018LORR0152/document.

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Le laboratoire a identifié récemment la protéine DDB2 comme ayant une activité dans la régulation de l’expression de gènes cibles tels que SOD2, BCL2 et NFKBIA, conférant ainsi à cette protéine un rôle dans le contrôle de la progression métastatique et dans la réponse thérapeutique des cellules tumorales mammaires. Cependant, l’activité réelle de DDB2 dans la transcription génique reste à définir, car sa structure ne permet pas d’expliquer son influence sur l’expression de ses gènes cibles. Cette activité peut être soit inhibitrice comme pour SOD2 et BCL2, soit activatrice comme NFKBIA qui cod

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Chan, Chen Hui. "Theoretical Modeling from Functionalized Gold Nanoparticles to Repair of Lesions in DNA for cancer radiotherapy." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSEN020.

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Le potentiel des nanoparticules d'or (AuNPs) pour améliorer l’efficacité de la radiothérapie est démontré par de nombreuses études expérimentales in vivo et in vitro. Ces particules métalliques augmentent significativement l’effet de la radiosensibilisation. La réaction en jeu est la radiolyse de l’eau: une fois excitées par un rayon X, elles génèrent des espèces réactives oxygénées qui amplifient les dégâts d’ADN et mènent à une plus grande destruction des cellules cancéreuses. Cependant, pour une efficacité thérapeutique plus optimale, plusieurs propriétés des AuNPs doivent être prises en co

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Cornu, Florian. "Validation in silico de la valeur prédictive du gène codant la protéine DDB2 (Damaged-DNA Binding 2) et caractérisation in vitro des effets de complexes organométalliques dans des modèles de cancers du sein." Thesis, Université de Lorraine, 2020. http://www.theses.fr/2020LORR0146.

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Le cancer du sein, malgré de nettes améliorations dans son diagnostic et sa prise en charge thérapeutique, reste la 1ère cause de décès chez les femmes. Différents paramètres permettent d’expliquer cette forte mortalité, tels que le manque actuel de biomarqueurs permettant de prédire la progression de la maladie. Des données préliminaires acquises par le laboratoire ont permis d’identifier la protéine DDB2 (Damaged-DNA binding 2) comme potentiel candidat pour la prédiction de la progression du cancer du sein. Une analyse in silico de bases de données transcriptomiques a permis de valider le rô

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Sun, Nian Kang, and 孫念康. "Functional studies on damaged-DNA recognition proteins." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/90720017755692168577.

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博士<br>國立清華大學<br>生命科學系<br>90<br>Damaged DNA-binding (DDB) activity comprises two major protein components, damaged DNA-binding protein 1 (DDB1) and 2 (DDB2). Those are implicated in the repair of ultraviolet (UV) radiation—induced DNA damage. To assess the functional correlation between DDBs and UV-damaged-DNA recognition activity, we identified UV-damaged-DNA recognition activities in rodent cell lines. There is a cell type-dependent expression of DDB1 and DDB2. Rodent cells had less abundant DDBs and lower UV-damaged-DNA recognition activity than did human tumor cells. Interestingly, the prof

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Ottoni, C., Hannah E. C. Koon, M. J. Collins, K. E. H. Penkman, O. Rickards, and O. E. Craig. "Preservation of ancient DNA in thermally damaged archaeological bone." 2009. http://hdl.handle.net/10454/6120.

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Evolutionary biologists are increasingly relying on ancient DNA from archaeological animal bones to study processes such as domestication and population dispersals. As many animal bones found on archaeological sites are likely to have been cooked, the potential for DNA preservation must be carefully considered to maximise the chance of amplification success. Here, we assess the preservation of mitochondrial DNA in a medieval cattle bone assemblage from Coppergate, York, UK. These bones have variable degrees of thermal alterations to bone collagen fibrils, indicative of cooking. Our results sho

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Kuo, Weihong. "The ING1b protein facilitates accessibility to UV-damaged nucleosomal DNA." Thesis, 2006. http://hdl.handle.net/2429/18330.

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ING1b is the most studied ING-family member protein and perhaps the most ubiquitous and highly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bulky nucleic acid photoproducts. We provide evidence that ING1b mediates nucleotide excision repair by facilitating access to damaged nucleosomal DNA. Our data suggest that this enhancement effect precedes the actions of damage recognition factor XPC, implicating a role of ING1b i

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Roth, Heide Marie. "Nucleotide Excision Repair: From Recognition to Incision of damaged DNA." Doctoral thesis, 2011. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-57098.

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The Nucleotide Excision Repair (NER) pathway is able to remove a vast diversity of structurally unrelated DNA lesions and is the only repair mechanism in humans responsible for the excision of UV induced DNA damages. The NER mechanism raises two fundamental questions: 1) How is DNA damage recognition achieved discriminating damaged from non damaged DNA? 2) How is DNA incision regulated preventing endonucleases to cleave DNA non specifically but induce and ensure dual incision of damaged DNA? Thus, the aim of this work was to investigate the mechanisms leading from recognition to incision of da

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Ho, Jhon-Chung, and 何炯昌. "Purification of UV-damaged-DNA Binding Proteins from Unicellular Algae Chlorella pyrenoidosa." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/97468926260343466979.

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Zhang, Zi-Wei, and 張自偉. "Nucleatide excision repair of UV-damaged DNA in chlorella prrengidosa cell-free extractsz." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/89598139618395959494.

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Al, Khateeb Wesam. "The role of DET1 and damaged DNA binding proteins (DDB1 & DDB2) in Arabidopsis DNA repair and light signaling." 2008. http://hdl.handle.net/1993/21130.

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Lysetska, Marina [Verfasser]. "Intact and damaged DNA and their interaction with DNA-binding proteins : a single molecule approach / submitted by Marina Lysetska." 2005. http://d-nb.info/975952560/34.

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SAN, PIETRO DAVID. "An Assessment of in vitro DNA Repair Mechanisms as Related to Damaged Forensic Specimens." Doctoral thesis, 2016. http://hdl.handle.net/11562/941658.

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I campioni forensi sottoposti ad indagini per il DNA sono spesso danneggiati. Per questo motivo sono stati sviluppati cocktail enzimatici che rispecchiano il meccanismo in vivo di riparazione del DNA. E' stato sviluppato un cocktail di questo tipo al fine di sviluppare un kit per la riparazione delle regioni di interesse al fine di ottenere soddisfacenti profili STR. Campioni di DNA sono stati estratti da cellule della mucosa buccale e danneggiati mediante varie esposizioni a raggi UV. Dagli stessi sono quindi stati ottenui i profili che sono stati confrontati con quelli ottenuti da campioni

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Paul, Vikram, and 維克朗. "Detrimental Effects of Mercury(II) and Arsenite on DNA Mismatch and UV-damaged-DNA Binding Activities in Zebrafish (Danio rerio) Embryos." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/5b42at.

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碩士<br>國立臺灣海洋大學<br>生命科學暨生物科技學系<br>104<br>ABSTRACT DNA Mismatch repair (MMR) and Nucleotide Excision Repair (NER) are the two DNA repair pathways that respectively corrects base mispairs generated during replication and removes helix distorting lesions such as UV- induced dipyrimidine photoproducts. Arsenic (As), Mercury (Hg) and Cadmium (Cd) are well known genotoxicants present in the aquatic environment. Inhibition of DNA damage repair has been proposed as one of the mechanisms of metal-induced genotoxicity. Because the potentials of Cd and Hg (II) to inhibit MMR in zebrafish embryos by dist

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Sabatinos, Sarah Anne. "Investigation of the function of UV damaged DNA binding protein 1 (DDB1) in mammalian systems." 2005. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=370980&T=F.

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Bokelmann, Lukas. "Development of methods for the characterization and retrieval of damaged DNA from ancient biological material." 2020. https://ul.qucosa.de/id/qucosa%3A74736.

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Development of methods for the characterization and retrieval of damaged DNA from ancient biological material Over course of the last decade, the field of ancient DNA has been transformed by the advent of highthroughput sequencing. In specimens with exceptional DNA preservation, this allowed whole nuclear genomes of extinct organisms to be sequenced and analyzed. To deal with material with suboptimal DNA preservation and high levels of external modern DNA contamination as is common in intensively handled museum specimens, significant challenges remain. In this thesis I developed laboratory an

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Hsieh, Feng-Ju, and 謝豐如. "Recognition and incision of UV-damaged DNA by vitellogenin1-like proteins in zebrafish (Danio rerio) early embryos." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/99556250569472509731.

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碩士<br>國立臺灣海洋大學<br>生物科技研究所<br>94<br>ABSTRACT Dipyrimidine photoproducts induced by UV irradiation on DNA are primarily removed from UV-damaged DNA by nucleotide excision repair (NER). NER proteins homologous to the human XPA were not detected in the extracts of zebrafish (Danio rerio) embryos, yet these extracts were previously found to contain two 30 to 35 kDa UV-damaged-DNA binding proteins homologous to the 150 kDa vitellogenin1 (Vg1). The objectives of this research were to examine if multiple Vg1-like UV-binding factors existed in zebrafish embryos and whether these factors were involved i

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"Photophysical Properties and Applications of Fluorescent Probes in Studying DNA Conformation and Dynamics." Doctoral diss., 2015. http://hdl.handle.net/2286/R.I.29730.

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abstract: Fluorescence spectroscopy is a popular technique that has been particularly useful in probing biological systems, especially with the invention of single molecule fluorescence. For example, Förster resonance energy transfer (FRET) is one tool that has been helpful in probing distances and conformational changes in biomolecules. In this work, important properties necessary in the quantification of FRET were investigated while FRET was also applied to gain insight into the dynamics of biological molecules. In particular, dynamics of damaged DNA was investigated. While damages in DNA ar

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Huang, Hui-Ying, and 黃惠英. "influence of ATP on the binding of zebrafish(Danio rerio)vitellogenin1-like proteins to UV-damaged DNA and the action mechanism." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/00805333113191883959.

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碩士<br>國立臺灣海洋大學<br>生物科技研究所<br>95<br>Abstract In most organisms, cyclobutane pyrimidine dimmers (CPDs) and (6-4) photoproducts (6-4 PPs) produced after UV irradiation are removed from damaged DNA primarily by nucleotide excision repair (NER) that introduces a dual incision at both ends of the lesion after an ATP-dependent DNA zebrafish (Danio rerio) vitellogenin 1 (zfVg1) had been identified as the components of a UV-damaged-DNA binding activity highly expressed in zebrafish embryos at 12 hr post fertilization (hpf) (Lai et al., 2006). Because of the requirement of ATP for DNA unwinding during N

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Wei, Chen Meng, and 陳孟偉. "Employing MTT assay to analyze the surviving fraction of DNA-damaged cell irradiated by microwave and Extremely Low Frequency electromagnetic field." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/60626082322041533311.

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碩士<br>國立清華大學<br>原子科學系<br>88<br>Abstract All functions in biological system are almost accomplished by transduction of electromagnetic signal; the binding energy among these biological molecules is larger than that of microwaves and extremely low frequency(ELF) electromagnetic(EM) fields. The energy of EM waves is incapable of breaking the bindings, but affect the electrons within the molecules for their polarization and drift, etc. So the cell properties may be influenced by the EM waves. In this dissertation, we utilized the free radicals that were generated by from Co-6

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Tung, Hsiu-Hui, and 董秀蕙. "Functional studies of the human damaged-DNA binding protein 2 (DDB2) and its associated protein B cell early response gene (BERG36)." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/58008185362829764486.

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碩士<br>國立成功大學<br>分子醫學研究所<br>95<br>DDB2 is an essential subunit of the damaged-DNA binding protein (DDB), which is involved in nucleotide excision repair (NER) in human cells. DDB2, together with DDB1, comprises the UV-DDB complex, which recognizes DNA lesions caused by UV irradiation. Mutations in the human DDB2 gene give rise to xeroderma pigmentosum group E (XPE), a disease characterized by increased skin tumorigenesis in response to UV-irradiation. Similarly to XPE patients, the DDB2-deficient mice developed spontaneous tumors. DDB2 is also shown to involve in p53-mediated apoptosis. Therefo

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