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Journal articles on the topic 'DNA extract'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Kubota, Y., and H. Takisawa. "Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts." Journal of Cell Biology 123, no. 6 (1993): 1321–31. http://dx.doi.org/10.1083/jcb.123.6.1321.

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Xenopus egg extracts prepared before and after egg activation retain M- and S-phase specific activity, respectively. Staurosporine, a potent inhibitor of protein kinase, converted M-phase extracts into interphase-like extracts that were capable of forming nuclei upon the addition of sperm DNA. The nuclei formed in the staurosporine treated M-phase extract were incapable of replicating DNA, and they were unable to initiate replication upon the addition of S-phase extracts. Furthermore, replication was inhibited when the staurosporine-treated M-phase extract was added in excess to the staurospor

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2

Skała, Ewa, Przemysław Sitarek, Marek Różalski, et al. "Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots ofRhaponticum carthamoidesagainst Induced Oxidative Stress and DNA Damage in CHO Cells." Oxidative Medicine and Cellular Longevity 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/5753139.

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Rhaponticum carthamoideshas a long tradition of use in Siberian folk medicine. The roots and rhizomes of this species are used in various dietary supplements or nutraceutical preparations to increase energy level or eliminate physical weakness. This is the first report to reveal the protective and DNA repair stimulating abilities ofR. carthamoidesroot extracts in Chinese hamster ovary (CHO) cells exposed to an oxidative agent. Both transformed root extract (TR extract) and extract of soil-grown plant roots (NR extract) may be responsible for stimulating CHO cells to repair oxidatively induced

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Nidugala, Hema, Ashwini Prabhu, Ramakrishna Avadhani, and Ravishankar B. "In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis." Biomedicine 43, no. 4 (2023): 1198–202. http://dx.doi.org/10.51248/.v43i4.2309.

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Introduction and Aim: Cyperus rotundus (L.), commonly known as nutgrass, has been in use in Indian medicine traditional system against several ailments. This investigation was performed to assess the in vitro antineoplastic activity of extracts prepared from water and ethanol with C. rotundus rhizomes. Materials and Methods: Toxic effects induced by the C. rotundus extracts on MCF-7 breast adenocarcinoma cells was evaluated using Sulphorhodamine- B (SRB) Assay, Trypan blue assay, Hoechst nuclear staining assay, Acridine Orange-Ethidium bromide (AO-EB) differential fluorescence staining assay a

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Ibrahim, Farah Wahida, Nurul Amirah Abdul Aziz, Latifah Ibrahim, Nurul Farhana Jufri, and Asmah Hamid. "Zingiber zerumbet (L.) Smith Hexane Crude Extract Caused DNA Damage on Leptospira spp." Sains Malaysiana 50, no. 10 (2021): 3085–94. http://dx.doi.org/10.17576/jsm-2021-5010-21.

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Numerous attempts have been made to control leptospirosis by using chemoprophylaxis, but with limited success. The present study was done to investigate the antileptospiral potential of hexane, ethyl acetate and methanol extracts of Zingiber zerumbet rhizomes. The extracts were assayed for antileptospiral activity using broth microdilution method against Leptospira interrogans (serovar Batavie, Canicola, Australis) and Leptospira biflexa serovar Patoc. The Z. zerumbet hexane extract exhibited antileptospiral activity, with IC50 values of 248 μg/mL against L. interrogans serovar Canicola, IC50

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Neslihan, GULEC, GOK Bahar, INAN Pinar, and ERDUGAN Huseyin. "Determination of antimicrobial activities and DNA protection properties of Scytosiphon lomentaria extracts." GSC Biological and Pharmaceutical Sciences 29, no. 2 (2024): 008–14. https://doi.org/10.5281/zenodo.14738356.

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<em>Scytosiphon lomentaria </em>is a brown algae that has many promising biological activities in medicine as well as being consumed as food in many countries. This study aims to investigate the antimicrobial, antimutagenic and DNA damage protective activity of <em>S. lomentaria</em> by obtaining hexane, methanol and water extracts. To evaluate the antimicrobial effect of the extracts, minimum inhibitory concentration (MIC) tests were performed on both yeasts and bacteria. The antimutagenic activities of the extracts were investigated by the Ames test, and their protective effec

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Kaygısız, Mehmet, and Eda Sönmez Gürer. "Determination of the antimicrobial, antioxidant activities and effects on oxidative DNA damage of extracts from three different Salvia species grown in Turkey." Prospects in Pharmaceutical Sciences 23, no. 1 (2025): 1–8. https://doi.org/10.56782/pps.300.

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Salvia officinalis L., known as medicinal sage, Salvia triloba L., known as Anatolian sage, and Salvia sclarea L., known as clary sage, belong to the Lamiaceae family and are species that typically grow in the Mediterranean region. This study aims to investigate the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) values, antioxidant and antimicrobial activities, total flavonoid and total phenolic content, and protective effects against oxidative DNA damage of extracts obtained from the aerial parts of three sage species — S. officinalis, S. triloba, and

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Ward, Peter, and R. Michael Linden. "A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication." Journal of Virology 74, no. 2 (2000): 744–54. http://dx.doi.org/10.1128/jvi.74.2.744-754.2000.

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ABSTRACT Assays have been described in which duplex adeno-associated virus (AAV) DNA can be replicated in HeLa cell extracts with exogenous AAV Rep protein. These assays appear to mimic the AAV DNA replication that occurs in the cell, including the ability of extracts from adenovirus (Ad)-infected cells to replicate duplex AAV DNA templates more efficiently than extracts from uninfected cells can. We showed previously that the Ad-infected extract was able to support a more processive replication than the uninfected extract. When the Ad single-stranded DNA binding protein (Ad-DBP) was added to

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Rajkumar, Venkatadri, Gunjan Guha, and Rangasamy Ashok Kumar. "Antioxidant and Anti-Cancer Potentials ofRheum emodiRhizome Extracts." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–9. http://dx.doi.org/10.1093/ecam/neq048.

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The objective of this study was to determine antioxidant and cytotoxic efficacies of methanolic and aqueous extracts ofRheum emodiWall. ex Meissn. rhizome. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, inhibitory effect on lipid peroxidation and Fe3+reducing antioxidant property have been used to investigate antioxidant properties of the extracts. Cytotoxicity of the extracts was tested on MDA-MB-435S and Hep3B cell lines. Both extracts displayed extensive cytotoxicity to the tested cell lines. The extracts were studied for their ability to protect pBR322 DNA

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Rajesh, Kowti, Joshi Vedamurthy, Kumar Mani Rupesh, et al. "UV rays induced DNA damage: Protection by polyphenols enriched extract of mint leaves." World Journal of Advanced Research and Reviews 8, no. 2 (2020): 152–57. https://doi.org/10.5281/zenodo.4318600.

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DNA of live cells is damaged when exposed to harmful Ultraviolet rays.  If the cells are exposed to UV rays exposure time more duration of time, more thymine dimers are formed in the DNA and the greater the risk of an incorrect repair or a missed dimer.  To analyze the effect of UV rays Calf thymus DNA and prevention by polyphenol enriched extract of Mint leaves and also its non toxic nature. The above study was done by using submarine gel electrophoresis where UV rays are used to damage the DNA and BHA (400µM) used as positive control.   The polyphenol enriched extra

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SHAPIRO, DAVID J., PHILLIP A. SHARP, WALTER W. WAHLI, and MARTHA J. KELLER. "A High-Efficiency HeLa Cell Nuclear Transcription Extract." DNA 7, no. 1 (1988): 47–55. http://dx.doi.org/10.1089/dna.1988.7.47.

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Singh, Nitika, Vivek Kumar Gupta, Pawan K. Doharey, Neeharika Srivastava, Abhishek Kumar, and Bechan Sharma. "A Study on Redox Potential of Phytochemicals and their Impact on DNA." Journal of DNA And RNA Research 1, no. 2 (2020): 10–22. http://dx.doi.org/10.14302/issn.2575-7881.jdrr-20-3267.

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The phytochemicals are endowed with antioxidant activities because of the presence of plentiful polyphenols and many other phytochemicals. However, some recent reports have suggested that phytochemicals from certain plant species exhibit DNA damaging properties mainly due to presence of alkaloids. In the present study, pBR322, Salmonella typhi DNA, insect DNA and human DNA were treated with hexanolic extract of Argemone mexicana and Thevesia peruviana leaves to assess their DNA damaging abilities. Another set of experiments was carried out using the methanolic extracts of Citrus lemon leaves t

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Akeem, Akinboro, Rufai Muhamed Akinlabi, Ogunbameru Aanuoluwapo Dorcas, Oladosu Bolaji Esther, and Taiwo Modinat Opeyemi. "Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells." Asian Journal of Medical and Biological Research 7, no. 3 (2021): 249–59. http://dx.doi.org/10.3329/ajmbr.v7i3.56135.

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Toxicological evaluation of herbal medicines is necessary because of possible adverse effects that may be associated with their consumption. This study screened antimalarial herbal recipe (containing leaves of Azadirachta indica and stem-bark of Alstonia boonei) and its individual plant’s extract for DNA and chromosomes mutation potentials following the DNA fragmentation and Allium cepa assays. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) activity of the recipe and each extract was determined. The kinds of phytochemicals present in them were determined using the FTIR te

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Danjolli-Hashani, Dua, and Sebnem Selen Isbilir. "Effect of Olea europaea L. (Domat and Gemlik var.) leaves extract against in vitro oxidative DNA damage." Herba Polonica 70, no. 2 (2024): 9–20. http://dx.doi.org/10.5604/01.3001.0054.6433.

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Reactive oxygen species formed in living organisms from various factors, cause to DNA damage, cytotoxicity and cell death. This study aims to investigate protective effect of two varieties of olive leaves extract against oxidative DNA damage induced by Fenton reaction. Gemlik variety (black olive) extract was found to have a slightly stronger protective effect than Domat (green olive) variety extract against oxidative DNA damage. Gemlik variety extract until did not show toxic effect on healthy liver cell line (AML12), it had shown cytotoxic effect on liver cancer cell line (Hep3B). However, D

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Sivakumari, K. "PHYTOCHEMICAL PROFILING, ANTICANCER AND APOPTOTIC ACTIVITY OF GRAVIOLA (ANNONA MURICATA) FRUIT EXTRACT AGAINST HUMAN HEPATOCELLULAR CARCINOMA (HepG-2) CELLS." International Journal of Zoology and Applied Biosciences 5, no. 1 (2020): 32–47. https://doi.org/10.5281/zenodo.3735733.

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To examine the phyto-constituents present in A. muricata fruit pulp, and to assess the anticancer potential followed by apoptotic studies on HepG-2 cell line. Aqueous, Chloroform, Ethyl acetate, Hexane and Methanol extracts of dragon fruit pulp were analysed by qualitative phytochemical profiling and anti-proliferation activity. Further, the apoptotic study of methanol extract treated HepG-2 cells by nuclear staining using AO, DNA fragmentation, ATR and GC-MS spectral analysis for methanol extract was carried out. The preliminary phytochemical screening showed the presence of acids, alkaloids,

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Stillman, B. W., and Y. Gluzman. "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells." Molecular and Cellular Biology 5, no. 8 (1985): 2051–60. http://dx.doi.org/10.1128/mcb.5.8.2051-2060.1985.

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Soluble extracts prepared from the nucleus and cytoplasm of human 293 cells are capable of efficient replication and supercoiling of added DNA templates that contain the origin of simian virus 40 replication. Extracts prepared from human HeLa cells are less active than similarly prepared extracts from 293 cells for initiation and elongation of nascent DNA strands. DNA synthesis is dependent on addition of purified simian virus 40 tumor (T) antigen, which is isolated by immunoaffinity chromatography of extracts from cells infected with an adenovirus modified to produce large quantities of this

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Stillman, B. W., and Y. Gluzman. "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells." Molecular and Cellular Biology 5, no. 8 (1985): 2051–60. http://dx.doi.org/10.1128/mcb.5.8.2051.

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Soluble extracts prepared from the nucleus and cytoplasm of human 293 cells are capable of efficient replication and supercoiling of added DNA templates that contain the origin of simian virus 40 replication. Extracts prepared from human HeLa cells are less active than similarly prepared extracts from 293 cells for initiation and elongation of nascent DNA strands. DNA synthesis is dependent on addition of purified simian virus 40 tumor (T) antigen, which is isolated by immunoaffinity chromatography of extracts from cells infected with an adenovirus modified to produce large quantities of this

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Cox, L. S., and G. H. Leno. "Extracts from eggs and oocytes of Xenopus laevis differ in their capacities for nuclear assembly and DNA replication." Journal of Cell Science 97, no. 1 (1990): 177–84. http://dx.doi.org/10.1242/jcs.97.1.177.

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We describe a cell-free extract derived from the oocytes of Xenopus laevis. The oocyte extract is capable of decondensing sperm chromatin and of replicating single-stranded DNA in a semiconservative, aphidicolin-sensitive manner. In addition, oocyte extract supports the elongation phase of DNA synthesis in nuclei that have been preinitiated for replication. All of these properties are shared by previously described egg extracts. However, oocyte extracts differ from egg extracts in two important ways. First, they cannot support nuclear assembly, as visualised by phase-contrast, fluorescence and

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Sp, Soul Shekhar, Manjulatha K, and Satyanarayan Nd. "ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD." Asian Journal of Pharmaceutical and Clinical Research 10, no. 9 (2017): 197. http://dx.doi.org/10.22159/ajpcr.2017.v10i9.19548.

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Objective: Cancer has become the major disease by uncontrolled cell growth effecting large population on the globe. This study, deals with antiproliferative activity of different solvent extracts, viz., n-hexane (S1), dichloromethane (DCM) (S2), and methanol (S3) of Acacia farnesiana pod on four cancer cell lines, viz., chronic myelogenous leukemia (K562), breast cancer (MCF-7), hepatocellular carcinoma (HePG2), colorectal adenocarcinoma (Colo 205), and DNA cleavage activity of the extracts on CT-DNA.Methods: The antiproliferative study was performed by MTT assay and DNA cleavage studies of th

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Neslihan GULEC, Bahar GOK, Pinar INAN, and Huseyin ERDUGAN. "Determination of antimicrobial activities and DNA protection properties of Scytosiphon lomentaria extracts." GSC Biological and Pharmaceutical Sciences 29, no. 2 (2024): 008–14. http://dx.doi.org/10.30574/gscbps.2024.29.2.0399.

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Scytosiphon lomentaria is a brown algae that has many promising biological activities in medicine as well as being consumed as food in many countries. This study aims to investigate the antimicrobial, antimutagenic and DNA damage protective activity of S. lomentaria by obtaining hexane, methanol and water extracts. To evaluate the antimicrobial effect of the extracts, minimum inhibitory concentration (MIC) tests were performed on both yeasts and bacteria. The antimutagenic activities of the extracts were investigated by the Ames test, and their protective effects against oxidative DNA damage w

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Lerksuthirat, Tassanee, Rakkreat Wikiniyadhanee, Sermsiri Chitphuk, et al. "DNA Repair Biosensor-Identified DNA Damage Activities of Endophyte Extracts from Garcinia cowa." Biomolecules 10, no. 12 (2020): 1680. http://dx.doi.org/10.3390/biom10121680.

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Recent developments in chemotherapy focus on target-specific mechanisms, which occur only in cancer cells and minimize the effects on normal cells. DNA damage and repair pathways are a promising target in the treatment of cancer. In order to identify novel compounds targeting DNA repair pathways, two key proteins, 53BP1 and RAD54L, were tagged with fluorescent proteins as indicators for two major double strand break (DSB) repair pathways: non-homologous end-joining (NHEJ) and homologous recombination (HR). The engineered biosensor cells exhibited the same DNA repair properties as the wild type

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Maikai, Victor Ambrose, Beatty Viv Maikai, and Patricia Ishyaku Kobo. "In VitroEffect of Aqueous Extract and Fraction IV Portion ofXimenia americanaStem Bark onTrypanosoma congolenseDNA." Journal of Parasitology Research 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/904318.

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Trypanosomosis is a debilitating disease affecting mainly livestock and humans in tropical Africa. Chemically synthesized drugs and medicinal plants have been used in the treatment and control of this disease. In this study, thein vitroeffect of aqueous extracts and fraction IV extract ofXimenia americanastem bark onTrypanosoma congolenseDNA was investigated. The extracts were incubated with the parasitesin vitroat 300 mg/mL aqueous extract and 25 mg/mL fraction IV portion for 30, 60, and 120 mins. The DNA of the trypanosomes was isolated and digested using ECOR1 enzyme and subsequently PCR wa

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Meziti, Hicham, Hamama Bouriche, Seoussen Kada, Ibrahim Demirtas, Murat Kizil, and Abderrahmane Senator. "Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts." Journal of Pharmacy & Pharmacognosy Research 7, no. 1 (2019): 260–72. http://dx.doi.org/10.56499/jppres19.569_7.4.260.

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Context: Quercus ilex and Pinus halepensis are largely used in Algerian folk medicine as a remedy for different health problems. Aims: To determine the phytochemical composition as well as to evaluate the antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex root bark extract (QIE) and Pinus halepensis young ovulate cones extract (PHE). Methods: Methanolic extracts were prepared by maceration. Phenolic compounds and flavonoids were quantified spectrophotometrically and identified by HPLC analysis. In vitro antioxidant activity of the extracts was assessed by determining the DP

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Kamaliah, Kamaliah. "PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA." BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan 5, no. 1 (2017): 60. http://dx.doi.org/10.22373/biotik.v5i1.2975.

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DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Acehâ€™s cow and Maduraâ€™s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolati

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Agripino, Débora Gomes, Marcos Enoque Leite Lima, Marcelo Rogério da Silva, et al. "Screening of Brazilian plants for antimicrobial and dnadamaging activities: I. Atlantic rain forest . Ecological station juréia-itatins." Biota Neotropica 4, no. 2 (2004): 1–15. http://dx.doi.org/10.1590/s1676-06032004000200009.

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I. Atlantic Rain Forest . Ecological Station Juréia-Itatins) Crude extracts from 88 plant species occurring in São Paulo State (Brazil), collected in an Atlantic Forest region, have been screened for antimicrobial and DNA-damaging activities. Of the 114 extracts assayed for antibacterial activity, only the extracts from leaves and stems of Aspidosperma ramiflorum (Apocynaceae) showed a slight activity against Escherichia coli. In the antifungal assay with Candida albicans, no active extract was observed, while in the bioautography assay with Cladosporium sphaerospermum and C. cladosporioides 1

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Topcu, Zeki, Bintug Ozturk, Ozlem Kucukoglu, and Emrah Kilinc. "Flavonoids in Helichrysum pamphylicum Inhibit Mammalian Type I DNA Topoisomerase." Zeitschrift für Naturforschung C 63, no. 1-2 (2008): 69–74. http://dx.doi.org/10.1515/znc-2008-1-213.

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DNA topoisomerases are important targets for cancer chemotherapy. We investigated the effects of a methanolic extract of Helichrysum pamphylicum on mammalian DNA topoisomerase I via in vitro plasmid supercoil relaxation assays. The extracts manifested a considerable inhibition of the enzyme’s activity in a dose-dependent manner. We also performed a HPLC analysis to identify the flavonoid content of the H. pamphylicum extract and tested the identified flavonoids; luteolin, luteolin-4-glucoside, naringenin, helichrysinA and isoquercitrin, on DNA topoisomerase I activity. The measurement of the t

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PEQUENO, Maitê Alves, Marissa Rodrigues SILVESTRE, Isabela AMÊNDOLA, Célia Regina Gonçalves e. SILVA, Mariella Vieira Pereira LEÃO, and Silvana Soleo Ferreira dos SANTOS. "Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study." RGO - Revista Gaúcha de Odontologia 66, no. 2 (2018): 122–28. http://dx.doi.org/10.1590/1981-863720180002000033328.

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ABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of co

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Bassa, Obed Yakubu, Sayyadi Abdulkadir, Rabiatu Bako Suleiman, Sarah Ufedu Adejoh, Zainab Julde Rilwanu, and Anisa Garba Abdulkarim. "<i>In vitro</i> protective effect of <i>Telfairai occidentalis</i> (fluted pumpkin) leaves against oxidative DNA damage induced by reactive oxygen species." Science World Journal 19, no. 4 (2025): 1096–98. https://doi.org/10.4314/swj.v19i4.27.

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Oxidative DNA damage is an inevitable consequence of cellular metabolism leading to reactive products that cause cellular damage. Telfairai occidentalis is a tropical vine grown in West Africa as a vegetable and for its edible seeds. This study aimed to evaluate the in vitro protective effect of T. occidentalis leaves extract against DNA damage induced by Fenton’s system. T. occidentalis leaves were extracted using three solvents; water, n-butanol (n-BuOH) and ethyl acetate (EtOAc). pVax plasmid DNA (476 ng) was incubated with Fenton’s system (FeSO4/H2O2) in the presence/varying concentrations

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Silva, Amélia M., Sandra C. Silva, Jorge P. Soares, et al. "Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage." Plants 8, no. 12 (2019): 556. http://dx.doi.org/10.3390/plants8120556.

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Ginkgo biloba L. leaf extracts and herbal infusions are used worldwide due to the health benefits that are attributed to its use, including anti-neoplastic, anti-aging, neuro-protection, antioxidant and others. The aim of this study was to evaluate the effect of an aqueous Ginkgo biloba extract on HepG2 cell viability, genotoxicity and DNA protection against paraquat-induced oxidative damage. Exposure to paraquat (PQ), over 24 h incubation at 1.0 and 1.5 µM, did not significantly reduce cell viability but induced concentration and time-dependent oxidative DNA damage. Ginkgo biloba leaf extract

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Kwon, Yun-Suk, So-Young Chun, Min-Kyoung Kim, Hong-Yan Nan, ChuHee Lee, and Soyoung Kim. "Mistletoe Extract Targets the STAT3-FOXM1 Pathway to Induce Apoptosis and Inhibits Metastasis in Breast Cancer Cells." American Journal of Chinese Medicine 49, no. 02 (2021): 487–504. http://dx.doi.org/10.1142/s0192415x21500221.

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Mistletoe extracts (Viscum album L.) have been widely used as complementary and alternative medicines for the treatment of cancer, and their cytotoxic effects have been reported on various types of cancer. However, the molecular targets of mistletoe extracts have not been well studied. Herein, we investigated molecules associated with the in vitro and in vivo anticancer effects of mistletoe extract using 4T1 murine breast cancer cells. Mistletoe extract induced apoptosis and inhibited the signal transducer and activator of transcription3 (STAT3) phosphorylation. This inhibition was accompanied

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UWAYA, O. D. "FREE RADICAL SCAVENGING AND ANTIOXIDANT CAPACITY OF LEAF OF Combretum platypterum (Welw) Hutch and Dalzie." GVU Journal of Science, Health and Technology 9, no. 1 (2024): 53–61. https://doi.org/10.5281/zenodo.13334657.

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An antioxidant is a molecule capable of preventing or inhibiting the oxidation of other molecules. Itends these reactions by removing free radical intermediates and inhibiting other oxidation reactions. Combretumplatypterum is a plant that belongs to the family Combretaceae. Despite being used in ethnomedicine, thereis no report of the in vitro antioxidant and free radical scavenging activities of its leaves. This study is aimedat testing the in vitro antioxidant and free radical scavenging activities of Combretum platypterum leaves. Invitro antioxidants such as ascorbic acid, carotenoids, and

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Bhandari, Deepika. "Touch DNA: Revolutionizing Evidentiary DNA Forensics." International Journal of Forensic Sciences 8, no. 3 (2023): 1–8. http://dx.doi.org/10.23880/ijfsc-16000314.

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Touch DNA is an advanced technique widely employed in modern criminal justice systems in many developed countries. It aims to extract genetic information from biological substances, specifically the cells shed from the outermost layer of skin, that are left behind on touched objects. This method involves recovering trace amounts of DNA from the biological cells released during contact, even though the quantity is usually very low. The recovered DNA is further analyzed to generate a person's DNA profile. Since dead cells are not really visible to the naked eye, successfully locating and recover

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Matsumoto, Y., and D. F. Bogenhagen. "Repair of a synthetic abasic site involves concerted reactions of DNA synthesis followed by excision and ligation." Molecular and Cellular Biology 11, no. 9 (1991): 4441–47. http://dx.doi.org/10.1128/mcb.11.9.4441-4447.1991.

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A synthetic analog of an abasic site in DNA is efficiently repaired by a short-patch repair mechanism in soluble extracts of Xenopus laevis oocytes (Y. Matsumoto and D. F. Bogenhagen, Mol. Cell. Biol. 9:3750-3757, 1989). We present a detailed analysis of the repair mechanism, using extracts depleted of endogenous nucleotide pools. ATP was required for repair with a sharp optimal concentration of 5 mM. The initial rate of repair was increased by preincubation of the DNA in the extract in the presence of ATP. During this preincubation, the DNA was cleaved on the 5' side of the lesion by a class

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Matsumoto, Y., and D. F. Bogenhagen. "Repair of a synthetic abasic site involves concerted reactions of DNA synthesis followed by excision and ligation." Molecular and Cellular Biology 11, no. 9 (1991): 4441–47. http://dx.doi.org/10.1128/mcb.11.9.4441.

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A synthetic analog of an abasic site in DNA is efficiently repaired by a short-patch repair mechanism in soluble extracts of Xenopus laevis oocytes (Y. Matsumoto and D. F. Bogenhagen, Mol. Cell. Biol. 9:3750-3757, 1989). We present a detailed analysis of the repair mechanism, using extracts depleted of endogenous nucleotide pools. ATP was required for repair with a sharp optimal concentration of 5 mM. The initial rate of repair was increased by preincubation of the DNA in the extract in the presence of ATP. During this preincubation, the DNA was cleaved on the 5' side of the lesion by a class

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Debabrata, Routh Prasanta Mondal Indranil Chatterjee Bishan Sarkar Suchetan Sarkar Anurag Kayal. "Checking The Antimicrobial Activity of The Seed Extract of Nigella sativa Extract." International Journal of Pharmaceutical Sciences 2, no. 7 (2024): 1161–76. https://doi.org/10.5281/zenodo.12746381.

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Black cumin, scientifically known as Nigella sativa, has garnered attention for its potential antimicrobial properties. Our Study suggests that its active compounds, such as thymoquinone, thymol, and carvacrol, exhibit significant antimicrobial activity against a wide range of pathogens. Also other Studies have shown that black cumin extracts possess antibacterial effects against various bacteria, including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Additionally, it has demonstrated antifungal properties against Candida albicans and Aspergillus species. The mechanism

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Gull, Iram, Attia Javed, Muhammad Shahbaz Aslam, Roohi Mushtaq, and Muhammad Amin Athar. "Use ofMoringa oleiferaFlower Pod Extract as Natural Preservative and Development of SCAR Marker for Its DNA Based Identification." BioMed Research International 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/7584318.

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The use ofMoringa oleiferaas natural food preservative has been evaluated in the present study. In addition, for quality assurance, the study has also been focused on the shelf life of product to authenticate the identification of plant by development of DNA based marker. Among the different extracts prepared from flower pods ofMoringa oleifera, methanol and aqueous extract exhibited high antibacterial and antioxidant activity, respectively. The high phenolic contents (53.5±0.169 mg GAE/g) and flavonoid contents (10.9±0.094 mg QE/g) were also recorded in methanol and aqueous extract, respectiv

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Faller, Adam C., Subramanyam Ragupathy, Dhivya Shanmughanandhan, et al. "DNA Quality and Quantity Analysis of Camellia sinensis Through Processing from Fresh Leaves to a Green Tea Extract." Journal of AOAC International 102, no. 6 (2019): 1798–807. http://dx.doi.org/10.5740/jaoacint.18-0318.

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Background: Although there has been some success using DNA barcoding to authenticate raw natural health product (NHP) botanical ingredients, there are many gaps in our understanding of DNA degradation, which may explain low PCR and sequencing success in processed NHPs. Objective: In this study, we measured multiple DNA variables after each step in the processing of a green tea extract in order to document DNA quality and quantity. Methods: We sampled plant material after each step of green tea extract processing: five steps at a Chinese tea farm (n = 10) and five at an NHP processing facility

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Faller, Adam C., Subramanyam Ragupathy, Dhivya Shanmughanandhan, et al. "DNA Quality and Quantity Analysis of Camellia sinensis Through Processing from Fresh Leaves to a Green Tea Extract." Journal of AOAC INTERNATIONAL 102, no. 6 (2019): 1798–807. http://dx.doi.org/10.1093/jaoac/102.6.1798.

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Abstract Background: Although there has been some success using DNA barcoding to authenticate raw natural health product (NHP) botanical ingredients, there are many gaps in our understanding of DNA degradation, which may explain low PCR and sequencing success in processed NHPs. Objective: In this study, we measured multiple DNA variables after each step in the processing of a green tea extract in order to document DNA quality and quantity. Methods: We sampled plant material after each step of green tea extract processing: five steps at a Chinese tea farm (n = 10) and five at an NHP processing

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Russo, A., F. Bonina, R. Acquaviva, et al. "Red Orange Extract: Effect on DNA Cleavage." Journal of Food Science 67, no. 8 (2002): 2814–18. http://dx.doi.org/10.1111/j.1365-2621.2002.tb08821.x.

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Jothy, Subramanion L., Yeng Chen, Jagat R. Kanwar, and Sreenivasan Sasidharan. "Evaluation of the Genotoxic Potential againstH2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract ofPolyalthia longifoliaLeaf." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/925380.

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Medicinal plants have been used in medicoculturally diverse countries around the world, where it is a part of a time-honoured tradition that is respected even today.Polyalthia longifolialeaf extract has been previously reported as an efficient antioxidantin vitro. Hence, the genotoxic effects ofP. longifolialeaf were investigated by using plasmid relation, comet, andAllium cepaassay. In the presence of ∙OH radicals, the DNA in supercoil was start nicked into open circular form, which is the product of the single-stranded cleavage of supercoil DNA and quantified as fragmented separate bands on

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Ikhane, Albert Olufemi, Foluso Oluwagbemiga Osunsanmi, Rebamang Anthony Mosa, and Andrew Rowland Opoku. "Antibacterial Potential of Crude Extracts from Cylindrospermum alatosporum NR125682 and Loriellopsis cavernicola NR117881." Microorganisms 13, no. 1 (2025): 211. https://doi.org/10.3390/microorganisms13010211.

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The challenges of antimicrobial resistance (AMR) to human health have pushed for the discovery of a new antibiotics agent from natural products. Cyanobacteria are oxygen-producing photosynthetic prokaryotes found in a variety of water habitats. Secondary metabolites are produced by cyanobacteria to survive extreme environmental stress factors, including microbial competition. This study presents the antibacterial activity and mechanism of the crude extracts from Cylindrospermum alatosporum NR125682 (A) and Loriellopsis cavernicola NR117881 (B) isolated from freshwater. The cyanobacteria were i

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Kaewkod, Thida, Puangphaka Kumseewai, Sureeporn Suriyaprom, Varachaya Intachaisri, Nitsanat Cheepchirasuk, and Yingmanee Tragoolpua. "Potential therapeutic agents of Bombyx mori silk cocoon extracts from agricultural product for inhibition of skin pathogenic bacteria and free radicals." PeerJ 12 (June 17, 2024): e17490. http://dx.doi.org/10.7717/peerj.17490.

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Background Pathogenic bacteria are the cause of most skin diseases, but issues such as resistance and environmental degradation drive the need to research alternative treatments. It is reported that silk cocoon extract possesses antioxidant properties. During silk processing, the degumming of silk cocoons creates a byproduct that contains natural active substances. These substances were found to have inhibitory effects on bacterial growth, DNA synthesis, the pathogenesis of hemolysis, and biofilm formation. Thus, silk cocoon extracts can be used in therapeutic applications for the prevention a

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K., Sai Pavithra a. *. Jeyanthi Annadurai b. R. Ragunathan c. "ANTICANCER ACTIVITY AND DNA FRAGMENTATION ACTIVITY OF A. PALLENS EXTRACTS." Journal of Pharma Research 8, no. 5 (2019): 260–65. https://doi.org/10.5281/zenodo.2678006.

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<strong><em>ABSTRACT</em></strong> <strong><em>Objective</em></strong><em>: The present study is to evaluate the anticancer activity of the leaf extracts of A. Pallens. <strong>Methods</strong>: Water, methanol and chloroform extracts were analyzed for their potent anticancer activity. <strong>Results</strong>: The chloroform extract seems to be very good when compared to the water and methanol extracts. These may be attributed to the compounds present in A. Pallens. <strong>Conclusion</strong>: The anticancer property may be due to the presence of many natural compounds. </em> <strong><em>KEY

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Prajeetha, Gayathri Sanalkumar, Arya Krishnan Kalarikkal, Soni Kallamparambil Balakrishnan, Swapna Alex, Ancy Joseph, and Sindura Kunhikrishnan Premakumari. "Curcuma caesia rhizome extract shows selective cytotoxicity and antiproliferative effect against lung and breast cancer cell lines." Journal of Phytopharmacology 14, no. 2 (2025): 87–95. https://doi.org/10.31254/phyto.2025.14203.

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Background: Black turmeric (Curcuma caesia Roxb.) exhibits various pharmacological properties, including anticancer, antifungal, and antibacterial activities. Objective: This study evaluates the cytotoxic and antiproliferative potential of crude methanolic rhizome extracts of black turmeric in selected cell lines. Materials and methods: The crude methanolic extract was prepared by the Soxhlet extraction method. Short-term cytotoxicity was determined by Trypan blue exclusion method in murine cancer cells (DLA and EAC), whereas in vitro cytotoxicity was determined by MTT assay in human cancer ce

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Riffiani, Rini, Nunik Sulistinah, and Bambang Sunarko. "COMPARISON OF THREE DNA ISOLATION AND PURIFICATION METHODS OF BACTERIAL DNA." KnE Life Sciences 2, no. 1 (2015): 491. http://dx.doi.org/10.18502/kls.v2i1.199.

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<p>The objective of this study was to compare the efficiency of three methods of nucleic acids extraction from gram-positive bacteria by evaluating the quantity and purity of DNA extracts. Nucleic acids extraction of gram-positive bacteria is normally hampered by a thick and resistant cell wall. Gram positive bacteria usually have a thick cell wall consisting mainly of many layers of peptidoglycan, which is not easily destroyed. This paper compares the different procedures based on mechanical and enzymatic cell breakage to extract DNA from Rhodoccocus pyridinivorans using GES method, Ult

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Čermáková, Eliška, Kamila Zdeňková, Kateřina Demnerová, and Jaroslava Ovesná. "Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas." Czech Journal of Food Sciences 39, No. 5 (2021): 410–17. http://dx.doi.org/10.17221/24/2021-cjfs.

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The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality. Some biological materials contain compounds which inhibit the functioning of DNA polymerase and thus need to be removed as part of the DNA extraction procedure. The aim of the present experiments was to optimise the process of DNA isolation from various types of black, fruit and herbal teas. A comparison was made between two cetyltrimethylammonium bromide (CTAB)-based protocols and two commercially available DNA purification kits. The

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Wang, Z., X. Wu, and E. C. Friedberg. "DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae." Molecular and Cellular Biology 13, no. 2 (1993): 1051–58. http://dx.doi.org/10.1128/mcb.13.2.1051-1058.1993.

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Base excision repair is an important mechanism for correcting DNA damage produced by many physical and chemical agents. We have examined the effects of the REV3 gene and the DNA polymerase genes POL1, POL2, and POL3 of Saccharomyces cerevisiae on DNA repair synthesis is nuclear extracts. Deletional inactivation of REV3 did not affect repair synthesis in the base excision repair pathway. Repair synthesis in nuclear extracts of pol1, pol2, and pol3 temperature-sensitive mutants was normal at permissive temperatures. However, repair synthesis in pol2 nuclear extracts was defective at the restrict

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Wang, Z., X. Wu, and E. C. Friedberg. "DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae." Molecular and Cellular Biology 13, no. 2 (1993): 1051–58. http://dx.doi.org/10.1128/mcb.13.2.1051.

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Base excision repair is an important mechanism for correcting DNA damage produced by many physical and chemical agents. We have examined the effects of the REV3 gene and the DNA polymerase genes POL1, POL2, and POL3 of Saccharomyces cerevisiae on DNA repair synthesis is nuclear extracts. Deletional inactivation of REV3 did not affect repair synthesis in the base excision repair pathway. Repair synthesis in nuclear extracts of pol1, pol2, and pol3 temperature-sensitive mutants was normal at permissive temperatures. However, repair synthesis in pol2 nuclear extracts was defective at the restrict

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Gul-e-Saba Chaudhry, Nur Khairina Ahmed Sohimi, Habsah Mohamad, et al. "Induction of apoptosis by selected Xylocarpus sp.,fractions in the human cervical cancer cell line, HeLa." International Journal of Research in Pharmaceutical Sciences 11, no. 2 (2020): 2332–39. http://dx.doi.org/10.26452/ijrps.v11i2.2210.

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Cancer is unchecked growth of normal cells. The screening of new potential agents is vital to develop effective cytotoxic drugs with reduced side effects. Thecytotoxic effects, mode of cell death and screening of phytochemicals which induced cell death by the mangrove plant Xylocarpus granatum extract on the human hepatocellular carcinoma cell line was studied. The crude methanol extract of Xylocarpusgranatum(bark) partitioning produced the high yield in diethyl ether (54.08%)followed by butanol (37.12%) and aqueous (3.21%).The Xylocarpus granatum bark extract produced a cytotoxic effect with

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Akinboro, A., M. A. Rufai, R. A. Adeagbo, et al. "Evaluation of an Antimalarial Herbal Recipe and its Individual Plant Extracts for DNA Fragmentation and Clastogenicity Potential in Swiss Albino Mice." Nigerian Journal of Basic and Applied Sciences 29, no. 2 (2022): 89–101. http://dx.doi.org/10.4314/njbas.v29i2.9.

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In this study, water extracts of leaves of Azadirachta indica and stem bark of Alstonia boonei, and their recipe were evaluated at 25.0%, 50.0% and 100.0% following DNA fragmentation and micronucleus assays in Swiss albino mice. Distilled water and 0.05% sodium azide served as negative and positive controls, respectively. The levels of superoxide dismutase, catalase and malondialdehyde in the treated mice were determined. The FTIR technique was adopted to identify the functional groups of phytochemicals present, and the proximate constituents were also determined. The individual extracts of A.

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Donkor, Addai-Mensah, Martin Ntiamoah Donkor, Benjamin Ahenkorah, Kwadwo Asante Asare-Konadu, Emmanuel Asiedu, and Richard Mosobil. "Physiological Alterations due to Hepatotoxicity and the Protective Role of Cleome viscosa Linn Seed Extract in Experimental Animals." Scientific World Journal 2022 (December 29, 2022): 1–11. http://dx.doi.org/10.1155/2022/6132201.

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Liver ailment is a key public health menace, principally in developing nations. Quite a lot of medicinal florae have been identified to have liver shielding activities. The current study was designed to assess in vitro antioxidant and in vivo hepatoprotective activities of seed extracts of Cleome viscosa Linn. (Capparaceae). Phytochemical screening of C. viscosa seed ethanol extract was carried out. Free radical scavenging activity of crude seed extract of the plant was conducted using the DPPH assay method. DNA damage protection potential of the crude seed extract was carried out using extrac

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