Relevant bibliographies by topics / DNA fingerprinting of fungi / Journal articles
To see the other types of publications on this topic, follow the link: DNA fingerprinting of fungi.

Journal articles on the topic 'DNA fingerprinting of fungi'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'DNA fingerprinting of fungi.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Soll, David R. "The Ins and Outs of DNA Fingerprinting the Infectious Fungi." Clinical Microbiology Reviews 13, no. 2 (April 1, 2000): 332–70. http://dx.doi.org/10.1128/cmr.13.2.332.

Full text
Abstract:
SUMMARY DNA fingerprinting methods have evolved as major tools in fungal epidemiology. However, no single method has emerged as the method of choice, and some methods perform better than others at different levels of resolution. In this review, requirements for an effective DNA fingerprinting method are proposed and procedures are described for testing the efficacy of a method. In light of the proposed requirements, the most common methods now being used to DNA fingerprint the infectious fungi are described and assessed. These methods include restriction fragment length polymorphisms (RFLP), R
APA, Harvard, Vancouver, ISO, and other styles
2

DeScenzo, R. A. "Use of (CAT)5as a DNA Fingerprinting Probe for Fungi." Phytopathology 84, no. 5 (1994): 534. http://dx.doi.org/10.1094/phyto-84-534.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Meyer, Wieland, Anke Koch, Claudia Niemann, Birgit Beyermann, J�rg T. Epplen, and Thomas B�rner. "Differentiation of species and strains among filamentous fungi by DNA fingerprinting." Current Genetics 19, no. 3 (March 1991): 239–42. http://dx.doi.org/10.1007/bf00336493.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Lockhart, S. R., C. Pujol, S. Joly, and D. R. Soll. "Development and use of complex probes for DNA fingerprinting the infectious fungi." Medical Mycology 39, no. 1 (January 2001): 1–8. http://dx.doi.org/10.1080/mmy.39.1.1.8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

WATANABE, M., K. LEE, K. GOTO, S. KUMAGAI, Y. SUGITA-KONISHI, and Y. HARA-KUDO. "Rapid and Effective DNA Extraction Method with Bead Grinding for a Large Amount of Fungal DNA." Journal of Food Protection 73, no. 6 (June 1, 2010): 1077–84. http://dx.doi.org/10.4315/0362-028x-73.6.1077.

Full text
Abstract:
To identify a rapid method for extracting a large amount of DNA from fungi associated with food hygiene, extraction methods were compared using fungal pellets formed rapidly in liquid media. Combinations of physical and chemical methods or commercial kits were evaluated with 3 species of yeast, 10 species of ascomycetous molds, and 4 species of zygomycetous molds. Bead grinding was the physical method, followed by chemical methods involving sodium dodecyl sulfate (SDS), cetyl trimethyl ammonium bromide (CTAB), and benzyl chloride and two commercial kits. Quantity was calculated by UV absorbanc
APA, Harvard, Vancouver, ISO, and other styles
6

Chiang, Yu-Chung, Chang-Hung Chou, Shong Huang, and Tzen-Yuh Chiang. "Possible consequences of fungal contamination on the RAPD fingerprinting in Miscanthus (Poaceae)." Australian Journal of Botany 51, no. 2 (2003): 197. http://dx.doi.org/10.1071/bt02021.

Full text
Abstract:
Fungal contamination has been frequently reported in higher plants. In Miscanthus species, a wide range of fungal flora has also been recorded previously, including an investigation based on nrITS amplification. In order to understand the effects of the fungal genomes on the random amplified polymorphic DNA (RAPD) fingerprinting, callus specimens were obtained from the tissue culture of shoot apices of Miscanthus. RAPD fingerprinting with 60 oligoprimers was conducted with genomic DNA extracted from leaf tissue collected in the field and from the greenhouse, as well as callus derived from the
APA, Harvard, Vancouver, ISO, and other styles
7

Becerra-LopezLavalle, L. Augusto, Jennifer A. Saleeba, and Bruce R. Lyon. "Molecular identification of fungi isolated from stem tissue of Upland cotton (Gossypium hirsutum)." Australian Journal of Botany 53, no. 6 (2005): 571. http://dx.doi.org/10.1071/bt04092.

Full text
Abstract:
Molecular techniques such as restriction fragment length polymorphism (RFLP) analysis, random amplification of polymorphic DNA (RAPD) fingerprinting, and DNA sequencing and database comparison, were employed to identify fungi isolated from field-grown cotton plants (Gossypium hirsutum L.). DNA fragments of between 510 and 590 bp, representing the two rDNA (rDNA) internal transcribed spacers (ITS1 and ITS2) and the intervening 5.8S rRNA gene, were amplified from the fungi with eukaryotic consensus primers. Subsequent digestion with the restriction endonucleases AluI, CfoI, HaeIII, HinfI and Hpa
APA, Harvard, Vancouver, ISO, and other styles
8

Oh, S., D. P. Kamdem, D. E. Keathley, and K. H. Han. "Detection and Species Identification of Wood-Decaying Fungi by Hybridization of Immobilized Sequence-Specific Oligonucleotide Probes with PCR-Amplified Fungal Ribosomal DNA Internal Transcribed Spacers." Holzforschung 57, no. 4 (June 26, 2003): 346–52. http://dx.doi.org/10.1515/hf.2003.052.

Full text
Abstract:
SummaryWe developed an effective detection method for wood-decaying fungi by hybridization of immobilized Sequence-Specific Oligonucleotide Probes with florescent-labeled PCR-amplified fungal rDNA internal transcribed spacer sequences. This method takes advantage of both the sequence specificity of Southern blot hybridization and the sensitivity of the previously reported PCR-based fungal species identification methods. Bothin vitrocultured fungal strains and naturally decaying wood samples were used to demonstrate that this method is robust and practical for detection of incipient wood-decayi
APA, Harvard, Vancouver, ISO, and other styles
9

Gadkar, Vijay, Alok Adholeya, and T. Satyanarayana. "Randomly amplified polymorphic DNA using the M13 core sequence of the vesicular–arbuscular mycorrhizal fungi Gigaspora margarita and Gigaspora gigantea." Canadian Journal of Microbiology 43, no. 8 (August 1, 1997): 795–98. http://dx.doi.org/10.1139/m97-115.

Full text
Abstract:
Vesicular–arbuscular mycorrhizal (VAM) fungi are obligate symbionts, and a primary benefit provided to the host is the alleviation of stress. The recalcitrance of these fungi to grow in pure culture has spurred researchers to develop an alternative form of cultivation, namely the root organ culture (ROC). This synthetic form of production is new and efforts were made to use randomly amplified polymorphic DNA with the M13 minisatellite sequence as the polymerase chain reaction primer to look into polymorphism, if any, in the spores of Gigaspora margarita produced both in vitro and in situ (soil
APA, Harvard, Vancouver, ISO, and other styles
10

Witfeld, Frederick, Dominik Begerow, and Marco Alexandre Guerreiro. "Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil." Mycological Progress 20, no. 3 (March 2021): 325–39. http://dx.doi.org/10.1007/s11557-021-01674-z.

Full text
Abstract:
AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and
APA, Harvard, Vancouver, ISO, and other styles
11

Gonçalves, Micael F. M., Ana C. Esteves, and Artur Alves. "Revealing the hidden diversity of marine fungi in Portugal with the description of two novel species, Neoascochyta fuci sp. nov. and Paraconiothyrium salinum sp. nov." International Journal of Systematic and Evolutionary Microbiology 70, no. 10 (October 1, 2020): 5337–54. http://dx.doi.org/10.1099/ijsem.0.004410.

Full text
Abstract:
Fungi are ubiquitous organisms with a wide distribution in almost all ecosystems, including marine environments. Coastal and estuarine ecosystems remain poorly unexplored as fungal habitats, potentially harbouring a hidden diversity with important ecological roles. During an extensive survey of marine fungi in coastal and estuarine Portuguese environments, a collection of 612 isolates was obtained from water, algae, sponges and driftwood. From these, 282 representative isolates were selected through microsatellite-primed PCR (MSP-PCR) fingerprinting analysis, which were identified based on DNA
APA, Harvard, Vancouver, ISO, and other styles
12

Kunová, Simona, Eva Ivanišová, Jana Žiarovská, Lucia Zamiešková, Soňa Felšöciová, Anka Trajkovska Petkoska, Daniela Nikolovska Nedelkoska, and Miroslava Kačániová. "Differences between microbiota, phytochemical, antioxidant profile and dna fingerprinting of cabernet sauvignon grape from Slovakia and Macedonia." Potravinarstvo Slovak Journal of Food Sciences 14 (October 28, 2020): 945–53. http://dx.doi.org/10.5219/1353.

Full text
Abstract:
This study aimed to evaluate the microbiota, phytochemical, antioxidant profile and DNA fingerprinting of Cabernet Sauvignon grapes from Slovakia and R. North Macedonia. There were used two samples of grape berries (one sample from Slovakia and one from Macedonia). Each sample was analyzed in triplicate. The bacteria were cultivated on Plate count agar (PCA), microscopic filamentous fungi were cultivated on Malt extract agar (MEA). MALDI-TOF MS Biotyper mass spectrometry was used for the identification of microorganisms (bacteria and yeasts) and microscopic filamentous fungi with manuals. DPPH
APA, Harvard, Vancouver, ISO, and other styles
13

White, John, Jeppe Nielsen, and Anne Madsen. "Potential Respiratory Deposition and Species Composition of Airborne Culturable, Viable, and Non-Viable Fungi during Occupancy in a Pig Farm." Atmosphere 11, no. 6 (June 16, 2020): 639. http://dx.doi.org/10.3390/atmos11060639.

Full text
Abstract:
Fungal species composition and site of deposition within the airways affects whether diseases develop and where they may arise. The aim of this study is to obtain knowledge regarding the potential deposition of airborne culturable, viable, and non-viable fungi in the airways of pig farm workers, and how this composition changes over multiple sampling days. Airborne fungi were sampled using impactors and subsequently analyzed using amplicon sequencing and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) fingerprinting. The geometric mean aerodynamic di
APA, Harvard, Vancouver, ISO, and other styles
14

Kageyama, Stacie A., Nancy Ritchie Posavatz, Kirk E. Waterstripe, Sarah J. Jones, Peter J. Bottomley, Kermit Cromack, and David D. Myrold. "Fungal and bacterial communities across meadow–forest ecotones in the western Cascades of Oregon." Canadian Journal of Forest Research 38, no. 5 (May 2008): 1053–60. http://dx.doi.org/10.1139/x07-221.

Full text
Abstract:
Meadows are natural dynamic features of forested mountain landscapes of the Pacific Northwest. Proportions of meadows and forests change with environmental conditions and disturbance history. We investigated the belowground microbial communities associated with these two vegetation types and how they change across the meadow–forest transition at two sites in Oregon. Soils were sampled along replicate transects extending from meadow into forest. We quantified total bacterial and fungal biomass using direct microscopy and described the composition of bacterial and fungal communities using a DNA-
APA, Harvard, Vancouver, ISO, and other styles
15

Okuda, Toru, Mieko Yanagisawa, Fumihiro Fujimori, Yuri Nishizuka, Yuki Takehana, and Masato Sugiyama. "New isolation methods and polymerase chain reaction strain discrimination techniques for natural products screening programs." Canadian Journal of Botany 73, S1 (December 31, 1995): 946–54. http://dx.doi.org/10.1139/b95-343.

Full text
Abstract:
A new isolation method of fungi was established by using the Percoll density gradient centrifugation. We found that the difference in the specific gravity allowed us to separate mixtures of fungal spores of Aspergillus, Fusarium, Listeromyces, Penicillium, and Trichoderma. When this technique was applied to soil samples, a wider variety of fungi were isolated compared with those obtained from an ordinary serial dilution method. For screening purposes, it is necessary to eliminate redundant strains from the strains initially isolated. For the efficient discrimination of strains, the random ampl
APA, Harvard, Vancouver, ISO, and other styles
16

Prat, Chantal, Olaya Ruiz-Rueda, Rosalia Trias, Enriqueta Anticó, Dimitra Capone, Mark Sefton, and Lluís Bañeras. "Molecular Fingerprinting by PCR-Denaturing Gradient Gel Electrophoresis Reveals Differences in the Levels of Microbial Diversity for Musty-Earthy Tainted Corks." Applied and Environmental Microbiology 75, no. 7 (February 5, 2009): 1922–31. http://dx.doi.org/10.1128/aem.02758-08.

Full text
Abstract:
ABSTRACT The microbial community structure of cork with marked musty-earthy aromas was analyzed using denaturing gradient gel electrophoresis of amplified ribosomal DNA. Cork stoppers and discs were used for DNA extraction and were analyzed by using selective primers for bacteria and fungi. Stoppers clearly differed from discs harboring a different fungal community. Moreover, musty-earthy samples of both types were shown to have a specific microbiota. The fungi Penicillium glabrum and Neurospora spp. were present in all samples and were assumed to make only a small contribution to off-odor dev
APA, Harvard, Vancouver, ISO, and other styles
17

McDonald, B. A., R. E. Pettway, R. S. Chen, J. M. Boeger, and J. P. Martinez. "The population genetics of Septoria tritici (teleomorph Mycosphaerella graminicola)." Canadian Journal of Botany 73, S1 (December 31, 1995): 292–301. http://dx.doi.org/10.1139/b95-259.

Full text
Abstract:
The DNA-based markers of molecular genetics were combined with the analytical tools of population genetics to learn about the population biology of the wheat pathogen Mycosphaerella graminicola. DNA-based genetic markers, including restriction fragment length polymorphisms in nuclear and mitochondrial DNA, DNA fingerprints, and electrophoretic karyotypes were used in combination to show that the amount and distribution of genetic variation within and among field populations of M. graminicola is similar around the world. Measures of gametic disequilibrium suggested that the sexual stage of repr
APA, Harvard, Vancouver, ISO, and other styles
18

Verbruggen, Erik, Eiko E. Kuramae, Remy Hillekens, Mattias de Hollander, E. Toby Kiers, Wilfred F. M. Röling, George A. Kowalchuk, and Marcel G. A. van der Heijden. "Testing Potential Effects of Maize Expressing the Bacillus thuringiensis Cry1Ab Endotoxin (Bt Maize) on Mycorrhizal Fungal Communities via DNA- and RNA-Based Pyrosequencing and Molecular Fingerprinting." Applied and Environmental Microbiology 78, no. 20 (August 10, 2012): 7384–92. http://dx.doi.org/10.1128/aem.01372-12.

Full text
Abstract:
ABSTRACTThe cultivation of genetically modified (GM) crops has increased significantly over the last decades. However, concerns have been raised that some GM traits may negatively affect beneficial soil biota, such as arbuscular mycorrhizal fungi (AMF), potentially leading to alterations in soil functioning. Here, we test two maize varieties expressing theBacillus thuringiensisCry1Ab endotoxin (Bt maize) for their effects on soil AM fungal communities. We target both fungal DNA and RNA, which is new for AM fungi, and we use two strategies as an inclusive and robust way of detecting community d
APA, Harvard, Vancouver, ISO, and other styles
19

Trigiano, R. N., G. Caetano-Anollés, B. J. Bassam, and M. T. Windham. "309 GENOMIC ANALYSIS OF TWO FUNGI CAUSING ANTHRACNOSE OF DOGWOOD (CORNUS SPECIES) IN THE EASTERN UNITED STATES." HortScience 29, no. 5 (May 1994): 474e—474. http://dx.doi.org/10.21273/hortsci.29.5.474e.

Full text
Abstract:
DNA Amplification Fingerprinting (DAF) was used to characterize ten isolates of Discula destructiva Redlin and three isolates of an undescribed species of Discula, the causal organisms of dogwood (Cornus species) anthracnose. Isolates were obtained throughout the disease range in the eastern United States and DAF profiles generated with ten arbitrary oligonucleotide primers. Very few polymorphic loci (27/298) were detected between isolates of D. destructiva; whereas, a greater number were observed between and among the isolates of Discula species. Relationships among and between the two fungal
APA, Harvard, Vancouver, ISO, and other styles
20

Valinsky, Lea, Gianluca Della Vedova, Tao Jiang, and James Borneman. "Oligonucleotide Fingerprinting of rRNA Genes for Analysis of Fungal Community Composition." Applied and Environmental Microbiology 68, no. 12 (December 2002): 5999–6004. http://dx.doi.org/10.1128/aem.68.12.5999-6004.2002.

Full text
Abstract:
ABSTRACT Thorough assessments of fungal diversity are currently hindered by technological limitations. Here we describe a new method for identifying fungi, oligonucleotide fingerprinting of rRNA genes (OFRG). ORFG sorts arrayed rRNA gene (ribosomal DNA [rDNA]) clones into taxonomic clusters through a series of hybridization experiments, each using a single oligonucleotide probe. A simulated annealing algorithm was used to design an OFRG probe set for fungal rDNA. Analysis of 1,536 fungal rDNA clones derived from soil generated 455 clusters. A pairwise sequence analysis showed that clones with
APA, Harvard, Vancouver, ISO, and other styles
21

Gryta, Agata, and Magdalena Frąc. "Methodological Aspects of Multiplex Terminal Restriction Fragment Length Polymorphism-Technique to Describe the Genetic Diversity of Soil Bacteria, Archaea and Fungi." Sensors 20, no. 11 (June 9, 2020): 3292. http://dx.doi.org/10.3390/s20113292.

Full text
Abstract:
The molecular fingerprinting methods used to evaluate soil microbial diversity could also be used as effective biosensors for the purposes of monitoring ecological soil status. The biodiversity of microorganisms is a relevant index of soil activity and there is a necessity to develop tools to generate reliable results for an emerging approach in the field of environmental control using microbial diversity biosensors. This work reports a method under development for determining soil microbial diversity using high efficiency Multiplex PCR-Terminal Restriction Fragment Length Polymorphism (M-T-RF
APA, Harvard, Vancouver, ISO, and other styles
22

Campbell, Michael, Michael Campbell, Rachael Adams, Emily Dobry, Kara Dobson, Kara Dobson, Veronica Stefanick, and Jessica Till. "The Sprout Regulating Compound 1,4-Dimethylnaphthalene Exhibits Fungistatic Activity." Journal of Agronomy Research 1, no. 3 (January 10, 2019): 27–34. http://dx.doi.org/10.14302/issn.2639-3166.jar-18-2502.

Full text
Abstract:
The compound 1,4-dimethylnaphthalene, originally isolated from dormant potatoes, is currently in use as a commercial sprout inhibitor. Growers and processors report a reduction in fungal infections in potatoes treated with DMN resulting in increased yields. To assess the effects of DMN on fungal growth a culture of Fusarium oxysporum was isolated from potato tubers and identified via DNA fingerprinting using the 18ITS ribosomal region. Growth of F. oxysporum was inhibited by 31% after four days of exposure to DMN but overall rate of spore germination was not affected by DMN treatment. The grow
APA, Harvard, Vancouver, ISO, and other styles
23

Moon, Christina D., Brian A. Tapper, and Barry Scott. "Identification of Epichloë Endophytes In Planta by a Microsatellite-Based PCR Fingerprinting Assay with Automated Analysis." Applied and Environmental Microbiology 65, no. 3 (March 1, 1999): 1268–79. http://dx.doi.org/10.1128/aem.65.3.1268-1279.1999.

Full text
Abstract:
ABSTRACT Epichloë endophytes are a group of filamentous fungi that include both sexual (Epichloë) and asexual (Neotyphodium) species. As a group they are genetically diverse and form both antagonistic and mutualistic associations with temperate grasses. We report here on the development of a microsatellite-based PCR system for fingerprinting this group of fungi with template isolated from either culture or infected plant material. M13mp19 partial genomic libraries were constructed for size-fractionated genomic DNA from two endophyte strains. These libraries were screened with a mixture of DI
APA, Harvard, Vancouver, ISO, and other styles
24

Sudini, H., C. R. Arias, M. R. Liles, K. L. Bowen, and R. N. Huettel. "Comparison of Soil Fungal Community Structure in Different Peanut Rotation Sequences Using Ribosomal Intergenic Spacer Analysis in Relation to Aflatoxin-Producing Fungi." Phytopathology® 101, no. 1 (January 2011): 52–57. http://dx.doi.org/10.1094/phyto-03-10-0072.

Full text
Abstract:
The present study focuses on determining soil fungal community structure in different peanut-cropping sequences by using a high-resolution DNA fingerprinting technique: ribosomal intergenic spacer analysis (RISA). This study was initiated to determine fungal community profiles in four peanut-cropping sequences (continuous peanut, 4 years of continuous bahiagrass followed by peanut, peanut-corn-cotton, and peanut-cotton rotations), with a special focus to evaluate whether the profiles under investigation may have also indicated microbial differences that could affect Aspergillus flavus populati
APA, Harvard, Vancouver, ISO, and other styles
25

Meyer, Wieland, and Thomas G. Mitchell. "Polymerase chain reaction fingerprinting in fungi using single primers specific to minisatellites and simple repetitive DNA sequences: Strain variation inCryptococcus neoformans." Electrophoresis 16, no. 1 (1995): 1648–56. http://dx.doi.org/10.1002/elps.11501601273.

Full text
APA, Harvard, Vancouver, ISO, and other styles
26

Ahmad, Rana Zaheer, Fuad Ameen, Rida Khalid, Mousa A. Alghuthaymi, Reem Alsalmi, and Chunjie Li. "A Brief History of Endophyte Detection Techniques in Grasses." Sustainable Agriculture Research 8, no. 3 (July 27, 2019): 66. http://dx.doi.org/10.5539/sar.v8n3p66.

Full text
Abstract:
Endophytes are the plant mutualists that live asymptomatically inside plant tissue and are found in nearly whole plant kingdom. Endophytic fungi receive shelter and nutrition from host plants and in return provide great advantages to the host. Grasses are a useful forage species and are of great agricultural and socio-economic value. The presence of endophytes in these grasses provide protection, persistence and improved yield against herbivores, insects, pathogens, drought and several other biotic and abiotic stresses. This review summarizes traditional and modern molecular techniques to iden
APA, Harvard, Vancouver, ISO, and other styles
27

Rajala, Tiina, Mikko Peltoniemi, Taina Pennanen, and Raisa Mäkipää. "Relationship between wood-inhabiting fungi determined by molecular analysis (denaturing gradient gel electrophoresis) and quality of decaying logs." Canadian Journal of Forest Research 40, no. 12 (December 2010): 2384–97. http://dx.doi.org/10.1139/x10-176.

Full text
Abstract:
We investigated the fungal communities inhabiting decaying logs in a seminatural boreal forest stand in relation to host tree species, stage of decay, density, diameter, moisture, C to N ratio, Klason lignin content, and water- and ethanol-soluble extractives. Communities were profiled using denaturing gradient gel electrophoresis fingerprinting of the rDNA ITS1 region coupled with sequencing of fungal DNA extracted directly from the wood. In addition, polypore fruit bodies were inventoried. Logs from different tree species had different fungal communities and different physicochemical propert
APA, Harvard, Vancouver, ISO, and other styles
28

MacNeil, L., T. Kauri, and W. Robertson. "Molecular techniques and their potential application in monitoring the microbiological quality of indoor air." Canadian Journal of Microbiology 41, no. 8 (August 1, 1995): 657–65. http://dx.doi.org/10.1139/m95-091.

Full text
Abstract:
Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This review discusses the shortcomings of current methods of identification and detection and focuses on the potential for molecular techniques in this emerging field. Probe techniques, restriction endonuclease analysis, karyotyping, and DNA and polymerase chain reaction fingerprinting
APA, Harvard, Vancouver, ISO, and other styles
29

Pagano, M. C. "Rhizobia associated with neotropical tree Centrolobium tomentosum used in riparian restoration." Plant, Soil and Environment 54, No. 11 (December 2, 2008): 498–508. http://dx.doi.org/10.17221/436-pse.

Full text
Abstract:
<I>Centrolobium tomentosum</I> is a tropical legume tree indicated for functional and structural restoration of riparian areas. This study was conducted to characterize the rhizobia isolated from nodules of <I>C. tomentosum in situ</I> and to determine their capacity of renodulation, in an experimental area of land rehabilitation in the Rio Doce valley. Nodulation potential to inoculation with 2 selected fast-growing <I>Rhizobium</I> strains separately and a mixed inoculum of arbuscular mycorrhizal fungi was evaluated by the use of antibiotics resistance. Fl
APA, Harvard, Vancouver, ISO, and other styles
30

Wang, Xiaojing, Xinxin Wang, and Gu Feng. "Optimised nitrogen fertiliser management achieved higher diversity of arbuscular mycorrhiza fungi and high-yielding maize (Zea mays L.)." Crop and Pasture Science 66, no. 7 (2015): 706. http://dx.doi.org/10.1071/cp14160.

Full text
Abstract:
The integrated soil–crop system management (ISSM) approach can potentially mitigate the loss of biodiversity in agricultural landscapes, ensuring crop yield with lower nitrogen (N) fertiliser input and minimised environmental pollution. The aim of this study was to test the hypotheses that overuse of N fertiliser could reduce the biodiversity of arbuscular mycorrhizal fungi (AMF) and that ISSM could help to maintain higher AMF biodiversity than the conventionally managed system in maize (Zea mays L.). The AMF community composition under three different treatments (conventionally managed, N-opt
APA, Harvard, Vancouver, ISO, and other styles
31

Andrade, Orlando, Gastón Muñoz, Rafael Galdames, Paola Durán, and Rodrigo Honorato. "Characterization, In Vitro Culture, and Molecular Analysis of Thecaphora solani, the Causal Agent of Potato Smut." Phytopathology® 94, no. 8 (August 2004): 875–82. http://dx.doi.org/10.1094/phyto.2004.94.8.875.

Full text
Abstract:
The fungus Thecaphora solani (syn.: Angiosorus solani), the causal agent of potato smut, was cultivated in vitro for the first time. Teliospores obtained from galls of infected potato plants were used to inoculate commonly used solid and liquid media. The teliospores produced two kinds of vegetative tissue depending on the nutrient status of the media. A very slow radial-growing, hyaline, and septate mycelium, as usually seen in most of the in vitro-cultivated filamentous fungi, was obtained in wateragar medium after 30 to 40 days. On the other hand, a white, sponge-like mycelial mass was obta
APA, Harvard, Vancouver, ISO, and other styles
32

Goodwin, Stephen B., Jessica R. Cavaletto, Cees Waalwijk, and Gert H. J. Kema. "DNA Fingerprint Probe from Mycosphaerella graminicola Identifies an Active Transposable Element." Phytopathology® 91, no. 12 (December 2001): 1181–88. http://dx.doi.org/10.1094/phyto.2001.91.12.1181.

Full text
Abstract:
DNA fingerprinting has been used extensively to characterize populations of Mycosphaerella graminicola, the Septoria tritici blotch pathogen of wheat. The highly polymorphic DNA fingerprints of Mycosphaerella graminicola were assumed to reflect the action of transposable elements. However, there was no direct evidence to support that conclusion. To test the transposable element hypothesis, the DNA fingerprint probe pSTL70 was sequenced, along with three other clones from a subgenomic library that hybridized with pSTL70. Analysis of these sequences revealed that pSTL70 contains the 3′ end of a
APA, Harvard, Vancouver, ISO, and other styles
33

Pancher, Michael, Marco Ceol, Paola Elisa Corneo, Claudia Maria Oliveira Longa, Sohail Yousaf, Ilaria Pertot, and Andrea Campisano. "Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management." Applied and Environmental Microbiology 78, no. 12 (April 6, 2012): 4308–17. http://dx.doi.org/10.1128/aem.07655-11.

Full text
Abstract:
ABSTRACTWe studied the distribution of fungal endophytes of grapevine (Vitis viniferaL.) plants in a subalpine area of northern Italy, where viticulture is of high economic relevance. We adopted both cultivation-based and cultivation-independent approaches to address how various anthropic and nonanthropic factors shape microbial communities. Grapevine stems were harvested from several locations considering organic and integrated pest management (IPM) and from the cultivars Merlot and Chardonnay. Cultivable fungi were isolated and identified by internal-transcribed-spacer sequence analysis, usi
APA, Harvard, Vancouver, ISO, and other styles
34

Himmelreich, Uwe, Ray L. Somorjai, Brion Dolenko, Ok Cha Lee, Heide-Marie Daniel, Ronan Murray, Carolyn E. Mountford, and Tania C. Sorrell. "Rapid Identification of Candida Species by Using Nuclear Magnetic Resonance Spectroscopy and a Statistical Classification Strategy." Applied and Environmental Microbiology 69, no. 8 (August 2003): 4566–74. http://dx.doi.org/10.1128/aem.69.8.4566-4574.2003.

Full text
Abstract:
ABSTRACT Nuclear magnetic resonance (NMR) spectra were acquired from suspensions of clinically important yeast species of the genus Candida to characterize the relationship between metabolite profiles and species identification. Major metabolites were identified by using two-dimensional correlation NMR spectroscopy. One-dimensional proton NMR spectra were analyzed by using a staged statistical classification strategy. Analysis of NMR spectra from 442 isolates of Candida albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis resulted in rapid, accurate identification when compared
APA, Harvard, Vancouver, ISO, and other styles
35

Iquebal, Mir Asif, Sarika Jaiswal, Vineet Kumar Mishra, Rahul Singh Jasrotia, Ulavappa B. Angadi, Bhim Pratap Singh, Ajit Kumar Passari, et al. "Fungal Genomic Resources for Strain Identification and Diversity Analysis of 1900 Fungal Species." Journal of Fungi 7, no. 4 (April 12, 2021): 288. http://dx.doi.org/10.3390/jof7040288.

Full text
Abstract:
Identification and diversity analysis of fungi is greatly challenging. Though internal transcribed spacer (ITS), region-based DNA fingerprinting works as a “gold standard” for most of the fungal species group, it cannot differentiate between all the groups and cryptic species. Therefore, it is of paramount importance to find an alternative approach for strain differentiation. Availability of whole genome sequence data of nearly 2000 fungal species are a promising solution to such requirement. We present whole genome sequence-based world’s largest microsatellite database, FungSatDB having >1
APA, Harvard, Vancouver, ISO, and other styles
36

Buchan, Alison, Steven Y. Newell, Melissa Butler, Erin J. Biers, James T. Hollibaugh, and Mary Ann Moran. "Dynamics of Bacterial and Fungal Communities on Decaying Salt Marsh Grass." Applied and Environmental Microbiology 69, no. 11 (November 2003): 6676–87. http://dx.doi.org/10.1128/aem.69.11.6676-6687.2003.

Full text
Abstract:
ABSTRACT Both bacteria and fungi play critical roles in decomposition processes in many natural environments, yet only rarely have they been studied as an integrated microbial community. Here we describe the bacterial and fungal assemblages associated with two decomposition stages of Spartina alterniflora detritus in a productive southeastern U.S. salt marsh. 16S rRNA genes and 18S-to-28S internal transcribed spacer (ITS) regions were used to target the bacterial and ascomycete fungal communities, respectively, based on DNA sequence analysis of isolates and environmental clones and by using co
APA, Harvard, Vancouver, ISO, and other styles
37

Chou, Chang-Hung, Yu-Chung Chiang, and Tzen-Yuh Chiang. "Genetic variability and phytogeography of Miscanthus sinensis var. condensatus, an apomictic grass, based on RAPD fingerprints." Canadian Journal of Botany 78, no. 10 (October 1, 2000): 1262–68. http://dx.doi.org/10.1139/b00-102.

Full text
Abstract:
DNA fingerprinting using random amplified polymorphic DNA (RAPD) markers was employed to investigate the genetic variation within and among populations of Miscanthus Anderss. sinensis var. condensatus (Hack.) Makino, an apomictic grass distributed along the coasts of Taiwan and Ryukyu Islands. A total of 250 plants from three Taiwanese populations (Southeast Coast, Orchid Islet, and Green Islet) and two populations from Ryukyu (Ishigaki and Amami-O-Shima Islets) were sampled. The amplified products of 40 random primers showed monomorphic banding patterns within all populations as well as among
APA, Harvard, Vancouver, ISO, and other styles
38

Turzhanova, Ainur, Oxana N. Khapilina, Asem Tumenbayeva, Vladislav Shevtsov, Olesya Raiser, and Ruslan Kalendar. "Genetic diversity of Alternaria species associated with black point in wheat grains." PeerJ 8 (May 5, 2020): e9097. http://dx.doi.org/10.7717/peerj.9097.

Full text
Abstract:
The genus Alternaria is a widely distributed major plant pathogen that can act as a saprophyte in plant debris. Fungi of this genus frequently infect cereal crops and cause such diseases as black point and wheat leaf blight, which decrease the yield and quality of cereal products. A total of 25 Alternaria sp. isolates were collected from germ grains of various wheat cultivars from different geographic regions in Kazakhstan. We investigated the genetic relationships of the main Alternaria species related to black point disease of wheat in Kazakhstan, using the inter-primer binding site (iPBS) D
APA, Harvard, Vancouver, ISO, and other styles
39

Prince, James P., and Steven D. Tanksley. "Restriction fragment length polymorphisms in plant breeding and genetics." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 99, no. 3-4 (1992): 23–29. http://dx.doi.org/10.1017/s0269727000005479.

Full text
Abstract:
SynopsisThe usefulness of restriction fragment length polymorphisms (RFLPs) in plant breeding and genetics is discussed, with particular emphasis on tagging genes, map-based cloning, the assessment of genetic variability and distances, and comparative genome mapping.The Department of Plant Breeding and Biometry has currently established tight linkages between RFLPs and more than 20 genes of economic importance. Approximately half of these genes confer resistance to major pathogens including nematodes, bacteria, fungi, and viruses. Other genes tagged are involved in various aspects of crop qual
APA, Harvard, Vancouver, ISO, and other styles
40

Somai, Benesh M., Ralph A. Dean, Mark W. Farnham, Thomas A. Zitter, and Anthony P. Keinath. "Internal Transcribed Spacer Regions 1 and 2 and Random Amplified Polymorphic DNA Analysis of Didymella bryoniae and Related Phoma Species Isolated from Cucurbits." Phytopathology® 92, no. 9 (September 2002): 997–1004. http://dx.doi.org/10.1094/phyto.2002.92.9.997.

Full text
Abstract:
Didymella bryoniae (anamorph Phoma cucurbitacearum) is the causal agent of gummy stem blight, although other Phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. The molecular and phylogenetic relationships between D. bryoniae and these Phoma species are unknown. Isolates of D. bryoniae and Phoma obtained from cucurbits grown at various geographical locations in the United States were subjected to random amplified polymorphic DNA (RAPD) analysis and internal transcribed spacer (ITS) sequence analysis (ITS-1 and ITS-2) to determine the molecular and phylogen
APA, Harvard, Vancouver, ISO, and other styles
41

Trung, Trịnh Thành, Đinh Thị Tuyết Vân, Nguyễn Phương Liên, Đào Thị Lương, and Dương Văn Hợp Dương Văn Hợp Dương Văn Hợp. "Potential application on preparation for bio-fertilizer using Bacillus velezensis strains isolated from various regions in Vietnam." Vietnam Journal of Biotechnology 15, no. 1 (April 20, 2018): 169–79. http://dx.doi.org/10.15625/1811-4989/15/1/12332.

Full text
Abstract:
Bacillus velezensis is a species belonging to the bacterial group of B. subtilis species complex. Due to various beneficial traits to plants, the bacterium has been received considerable attention for application in disease control and crop productivity. In this study, we isolated 15 B. velezensis strains from various soils collected in regions of Hoang Lien, Cuc Phuong, Bach Ma, Chu Yang Sin and Con Dao. All of the strains demonstrated antagonistic activity against phythopathogenic fungi of Fusarium oxysporum, Sclerotium hydrophilum, Rhizoctonia solani and Phytophthora capsici and rice blight
APA, Harvard, Vancouver, ISO, and other styles
42

SENGUPTA, RAJIB, DHUNDY R. BASTOLA, and HESHAM H. ALI. "CLASSIFICATION AND IDENTIFICATION OF FUNGAL SEQUENCES USING CHARACTERISTIC RESTRICTION ENDONUCLEASE CUT ORDER." Journal of Bioinformatics and Computational Biology 08, no. 02 (April 2010): 181–98. http://dx.doi.org/10.1142/s0219720010004616.

Full text
Abstract:
Restriction Fragment Length Polymorphism (RFLP) is a powerful molecular tool that is extensively used in the molecular fingerprinting and epidemiological studies of microorganisms. In a wet-lab setting, the DNA is cut with one or more restriction enzymes and subjected to gel electrophoresis to obtain signature fragment patterns, which is utilized in the classification and identification of organisms. This wet-lab approach may not be practical when the experimental data set includes a large number of genetic sequences and a wide pool of restriction enzymes to choose from. In this study, we intr
APA, Harvard, Vancouver, ISO, and other styles
43

Köhl, J., B. H. Groenenboom-de Haas, P. Kastelein, V. Rossi, and C. Waalwijk. "Quantitative Detection of Pear-Pathogenic Stemphylium vesicarium in Orchards." Phytopathology® 99, no. 12 (December 2009): 1377–86. http://dx.doi.org/10.1094/phyto-99-12-1377.

Full text
Abstract:
Isolates of Stemphylium vesicarium causing brown spot of pear can be distinguished from nonpathogenic isolates of S. vesicarium from pear or from other hosts on the basis of distinctive amplified fragment length polymorphism fingerprinting profiles. DNA fragments specific for isolates pathogenic to pear were identified and a quantitative polymerase chain reaction (PCR) was developed on the sequence from one of these specific DNA loci. This TaqMan PCR has a high sensitivity with a dynamic range for reliable quantification between 1 ng and 100 fg of DNA. The method detected pear-pathogenic isola
APA, Harvard, Vancouver, ISO, and other styles
44

Rovná, Katarína, Eva Ivanišová, Jana Žiarovská, Peter Ferus, Margarita Terentjeva, Przemysław Łukasz Kowalczewski, and Miroslava Kačániová. "Characterization of Rosa canina Fruits Collected in Urban Areas of Slovakia. Genome Size, iPBS Profiles and Antioxidant and Antimicrobial Activities." Molecules 25, no. 8 (April 19, 2020): 1888. http://dx.doi.org/10.3390/molecules25081888.

Full text
Abstract:
The studies of plant bacterial endophytes, colonizing the plant tissues without any signs of diseases, are essential for understanding of ecological interactions. The aim of our study is to detect microbiological contamination and to assess the antimicrobial, antioxidant activity, total phenolic, carotenoid content, genome size, and ploidy of non-cultivated Rosa canina sampled from urban areas. Samples of Rosa canina fruits were collected in three locations in Slovakia. The highest total viable count and the Enterobacteriaceae count in fruits were 4.32 log CFU/g and 4.29 log CFU/g, respectivel
APA, Harvard, Vancouver, ISO, and other styles
45

Knapp, B. A., J. Seeber, S. M. Podmirseg, E. Meyer, and H. Insam. "Application of denaturing gradient gel electrophoresis for analysing the gut microflora ofLumbricus rubellusHoffmeister under different feeding conditions." Bulletin of Entomological Research 98, no. 3 (April 28, 2008): 271–79. http://dx.doi.org/10.1017/s0007485308006056.

Full text
Abstract:
AbstractThe earthworm,Lumbricus rubellus, plays an essential role in soil ecosystems as it affects organic matter decomposition and nutrient cycling. By ingesting a mixture of organic and mineral material, a variety of bacteria and fungi are carried to the intestinal tract of the earthworm. To get a better understanding of the interactions betweenL. rubellusand the microorganisms ingested, this study tried to reveal if the diet affects the composition of the gut microflora ofL. rubellusor if its intestinal tract hosts an indigenous, species-specific microbiota. A feeding experiment withL. rube
APA, Harvard, Vancouver, ISO, and other styles
46

Brown, Eric, and Emma Allen-Vercoe. "Analysis of the fungal, archaeal and bacteriophage diversity in the human distal gut." SURG Journal 4, no. 2 (March 11, 2011): 75–82. http://dx.doi.org/10.21083/surg.v4i2.1331.

Full text
Abstract:
The composition and role of bacteria in the human gut has been studied intensely and is a burgeoning field of scientific research. However, there is a relative lack of research on other microorganisms which compose our gut flora such as bacteriophage, archaea and fungi. The aim of our study was to begin to fill this gap. The archaeal, fungal and bacteriophage diversity in the gut was analyzed using a PCR-DGGE fingerprinting method on fecal samples from 3 healthy donors. These samples were inoculated into chemostats and the microbes were grown in continuous culture to model the interactions of
APA, Harvard, Vancouver, ISO, and other styles
47

du PLESSIS, ERIKA M., FRANCOIS DUVENAGE, and LISE KORSTEN. "Determining the Potential Link between Irrigation Water Quality and the Microbiological Quality of Onions by Phenotypic and Genotypic Characterization of Escherichia coli Isolates." Journal of Food Protection 78, no. 4 (April 1, 2015): 643–51. http://dx.doi.org/10.4315/0362-028x.jfp-14-486.

Full text
Abstract:
The potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in South Africa have increasingly been reported to be contaminated with enteric bacterial pathogens. A microbiological analysis was performed of a selected river in Limpopo Province, South Africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial farm. Counts of Escherichia coli, coliforms, aerobic bacteria, fungi, and yeasts and the prevalence o
APA, Harvard, Vancouver, ISO, and other styles
48

Vohník, Martin. "Bioerosion and fungal colonization of the invasive foraminiferan <i>Amphistegina lobifera</i> in a Mediterranean seagrass meadow." Biogeosciences 18, no. 8 (April 30, 2021): 2777–90. http://dx.doi.org/10.5194/bg-18-2777-2021.

Full text
Abstract:
Abstract. Foraminiferans are diverse micro- to macroscopic protists abundant especially in (sub)tropical seas, often forming characteristic benthic communities known as “living sands”. Numerous species have migrated through the Suez Canal to the Mediterranean and one of them, i.e., Amphistegina lobifera, turned invasive, gradually outcompeting the indigenous species. At some places, A. lobifera creates thick seabed sediments, thus becoming an important environmental engineer. However, little is known about the turnover of its shells in the invaded ecosystems. Using vital staining, stereomicros
APA, Harvard, Vancouver, ISO, and other styles
49

ben Omar, Nabil, and Fr�d�ric Ampe. "Microbial Community Dynamics during Production of the Mexican Fermented Maize Dough Pozol." Applied and Environmental Microbiology 66, no. 9 (September 1, 2000): 3664–73. http://dx.doi.org/10.1128/aem.66.9.3664-3673.2000.

Full text
Abstract:
ABSTRACT The dynamics of the microbial community responsible for the traditional fermentation of maize in the production of Mexican pozol was investigated by using a polyphasic approach combining (i) microbial enumerations with culture media, (ii) denaturing gradient gel electrophoresis (DGGE) fingerprinting of total community DNA with bacterial and eukaryotic primers and sequencing of partial 16S ribosomal DNA (rDNA) genes, (iii) quantification of rRNAs from dominant microbial taxa by using phylogenetic oligonucleotide probes, and (iv) analysis of sugars and fermentation products. AStreptococ
APA, Harvard, Vancouver, ISO, and other styles
50

de Boer, Wietse, Johan H. J. Leveau, George A. Kowalchuk, Paulien J. A. Klein Gunnewiek, Edwin C. A. Abeln, Marian J. Figge, Klaas Sjollema, Jaap D. Janse, and Johannes A. van Veen. "Collimonas fungivorans gen. nov., sp. nov., a chitinolytic soil bacterium with the ability to grow on living fungal hyphae." International Journal of Systematic and Evolutionary Microbiology 54, no. 3 (May 1, 2004): 857–64. http://dx.doi.org/10.1099/ijs.0.02920-0.

Full text
Abstract:
A polyphasic approach was used to describe the phylogenetic position of 22 chitinolytic bacterial isolates that were able to grow at the expense of intact, living hyphae of several soil fungi. These isolates, which were found in slightly acidic dune soils in the Netherlands, were strictly aerobic, Gram-negative rods. Cells grown in liquid cultures were flagellated and possessed pili. A wide range of sugars, alcohols, organic acids and amino acids could be metabolized, whereas several di- and trisaccharides could not be used as substrates. The major cellular fatty acids were C16 : 0, C16 : 1 ω7
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the bibliographies on the topic 'DNA fingerprinting of fungi' for other source types:
Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography