Relevant bibliographies by topics / DNA-free gene editing

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters

Academic literature on the topic 'DNA-free gene editing'

Author: Grafiati
Published: 11 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "DNA-free gene editing"

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Haas, Amanda. "DNA-Free CRISPR-Cas9 Gene Editing." Genetic Engineering & Biotechnology News 36, no. 17 (2016): 16–17. http://dx.doi.org/10.1089/gen.36.17.07.

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Zhang, Yi, and Caixia Gao. "Recent advances in DNA-free editing and precise base editing in plants." Emerging Topics in Life Sciences 1, no. 2 (2017): 161–68. http://dx.doi.org/10.1042/etls20170021.

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Genome-editing technologies based on the CRISPR (clustered regularly interspaced short palindromic repeat) system have been widely used in plants to investigate gene function and improve crop traits. The recently developed DNA-free delivery methods and precise base-editing systems provide new opportunities for plant genome engineering. In this review, we describe the novel DNA-free genome-editing methods in plants. These methods reduce off-target effects and may alleviate regulatory concern about genetically modified plants. We also review applications of base-editing systems, which are highly
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He, Yubing, Michael Mudgett, and Yunde Zhao. "Advances in gene editing without residual transgenes in plants." Plant Physiology 188, no. 4 (2021): 1757–68. http://dx.doi.org/10.1093/plphys/kiab574.

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Abstract Transgene residuals in edited plants affect genetic analysis, pose off-target risks, and cause regulatory concerns. Several strategies have been developed to efficiently edit target genes without leaving any transgenes in plants. Some approaches directly address this issue by editing plant genomes with DNA-free reagents. On the other hand, DNA-based techniques require another step for ensuring plants are transgene-free. Fluorescent markers, pigments, and chemical treatments have all been employed as tools to distinguish transgenic plants from transgene-free plants quickly and easily.
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Tsanova, Tsveta, Lidia Stefanova, Lora Topalova, Atanas Atanasov, and Ivelin Pantchev. "DNA-free gene editing in plants: a brief overview." Biotechnology & Biotechnological Equipment 35, no. 1 (2020): 131–38. http://dx.doi.org/10.1080/13102818.2020.1858159.

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Brandt, Camilla Blunk, Sofie Vestergaard Fonager, János Haskó, et al. "HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes." Biomolecules 13, no. 1 (2022): 23. http://dx.doi.org/10.3390/biom13010023.

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Primary endothelial cells (ECs), especially human umbilical vein endothelial cells (HUVECs), are broadly used in vascular biology. Gene editing of primary endothelial cells is known to be challenging, due to the low DNA transfection efficiency and the limited proliferation capacity of ECs. We report the establishment of a highly efficient and selection-free CRISPR gene editing approach for primary endothelial cells (HUVECs) with ribonucleoprotein (RNP) complex. We first optimized an efficient and cost-effective protocol for messenger RNA (mRNA) delivery into primary HUVECs by nucleofection. Ne
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Nasri, Masoud, Perihan Mir, Benjamin Dannenmann, et al. "Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response." Blood Advances 3, no. 1 (2019): 63–71. http://dx.doi.org/10.1182/bloodadvances.2017015511.

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Abstract CRISPR/Cas9-mediated gene editing of stem cells and primary cell types has several limitations for clinical applications. The direct delivery of ribonucleoprotein (RNP) complexes consisting of Cas9 nuclease and guide RNA (gRNA) has improved DNA- and virus-free gene modifications, but it does not enable the essential enrichment of the gene-edited cells. Here, we established a protocol for the fluorescent labeling and delivery of CRISPR/Cas9–gRNA RNP in primary human hematopoietic stem and progenitor cells (HSPCs) and induced pluripotent stem cells (iPSCs). As a proof of principle for g
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Mushtaq, Muntazir, Aejaz Ahmad Dar, Milan Skalicky, et al. "CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges." Genes 12, no. 6 (2021): 797. http://dx.doi.org/10.3390/genes12060797.

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Genome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified organism (GMO), are of special interest. The recent and rapid developments in genome-editing technology have given rise to hopes to achieve global food security in a sustainable manner. We here discuss recent developments in CRISPR-based genome-editing tools for crop improvement concerning adaptation, opportunities, and challenges. Some of the nota
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Wang, Chengkun, Yuanhao Qu, Jason K. W. Cheng, et al. "dCas9-based gene editing for cleavage-free genomic knock-in of long sequences." Nature Cell Biology 24, no. 2 (2022): 268–78. http://dx.doi.org/10.1038/s41556-021-00836-1.

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AbstractGene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene editing could cause DNA damage and trigger DNA repair processes that are often error-prone. Such unwanted mutations and safety concerns can be exacerbated when altering long sequences. Here we couple microbial single-strand annealing proteins (SSAPs) with catalytically inactive dCas9 for gene editing. This cleavage-free gene editor, dCas9–SSAP, promotes the knock-in of long sequences in mammalian cells. The dCas9–SSAP editor has low on-target errors and minimal off-target effects, showing h
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Badhan, Sapna, Andrew S. Ball, and Nitin Mantri. "First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts." International Journal of Molecular Sciences 22, no. 1 (2021): 396. http://dx.doi.org/10.3390/ijms22010396.

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The current genome editing system Clustered Regularly Interspaced Short Palindromic Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and simplicity in several plant-based applications. Together with the availability of a vast amount of genome data and transcriptome data, CRISPR/Cas9 presents a massive opportunity for plant breeders and researchers. The successful delivery of ribonucleoproteins (RNPs), which are composed of Cas9 enzyme and a synthetically designed single guide RNA (sgRNA) and are used in combination with various transformation methods or lately av
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Ariga, Hirotaka, Seiichi Toki, and Kazuhiro Ishibashi. "Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants." Plant and Cell Physiology 61, no. 11 (2020): 1946–53. http://dx.doi.org/10.1093/pcp/pcaa123.

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Abstract Genome editing technology is important for plant science and crop breeding. Genome-edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA, which is problematic for the production of genome-edited transgene-free plants for vegetative propagation or highly heterozygous hybrid cultivars. Here, we describe a method for highly efficient targeted mutagenesis in Nicotiana benthamiana through the expression of Cas9 and single-guide (sg)RNA using a potato virus X (PVX) vector. Following Agrobacterium-mediated introduction of virus vector cDNA, >60% of shoot
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Dissertations / Theses on the topic "DNA-free gene editing"

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Najafi. "DNA-free Gene editing in Vitis vinifera L.; Knockout green fluorescent protein gene in Sultana grape by direct delivery of RNPs into protoplast." Doctoral thesis, 2022. http://hdl.handle.net/11562/1068065.

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Global agricultural productivity, farm incomes, and food security will all be impacted by climate change. Grape (Vitis vinifera L.) is one of the world's most commercially significant fruit crops, and it is extensively cultivated for fruits, juice, and, most importantly, wine. Scientific evidence sharply states that climate change represents a dominant challenge for viticulture in the upcoming decades. Agriculture and farming around the world are highly depended on crops that produce food and fiber for humans, either directly or indirectly through livestock. Modern technology has improved agri
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Books on the topic "DNA-free gene editing"

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Nettleton, Claire Correo, Ellen K. Levy, Molly Duggins, et al., eds. Art and Biotechnology. Bloomsbury Publishing Plc, 2024. http://dx.doi.org/10.5040/9781350376069.

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This interdisciplinary anthology examines the relationship between developments in biotechnology and both artistic and literary innovation, focussing in particular on how newfound molecular technologies and knowledge regimes, such as CRISPR gene editing, alter conceptions of what it means to be human. The book presents 21 essays, split across five parts, from a coterie of artists, scientists, and theorists, which examine the symbiotic relationship between humans, animals, and viruses as well as the impossibility of germ-free existence. The essays in this volume are urgent in their topicality,
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Book chapters on the topic "DNA-free gene editing"

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Bruegmann, Tobias, Alexander Fendel, Virginia Zahn, and Matthias Fladung. "Genome Editing in Forest Trees." In A Roadmap for Plant Genome Editing. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-46150-7_20.

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AbstractSince the first CRISPR/Cas-mediated genome editing of poplar in 2015, an increasing number of tree species are being genome-edited. Although the availability of genome sequences, tissue culture and transformation systems are limiting factors, research is ongoing on advanced methods such as DNA-free genome editing and gene targeting approaches in addition to the optimisation of single gene knockouts. These can be used to address ambitious issues and perform genome editing more accurately, which has implications for the legal assessment of edited trees. Once technically established, CRIS
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Śmiech, Magdalena, Paweł Leszczyński, Effi Haque, and Hiroaki Taniguchi. "Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detection." In Springer Protocols Handbooks. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0616-2_10.

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Yaseen, Saba, Azara Yasmeen, Naila Safdar, and Zulqurnain Khan. "Genome Editing and its Applications in Plants." In Medicinal Plants: Microbial Interactions, Molecular Techniques and Therapeutic Trends. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815136838123010012.

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Regularly interspaced short palindromic repeats/cas9 system (CRISPR-Cas) is a well-developed and frequently used genome editing system, which comprises a Cas9 nuclease and a single-guided RNA (that is an RNA-guided technique). Cas9 recognizes and cuts a specific DNA sequence by base-pairing with it, generating double-strand breakage (DSBs) that initiate cellular DNA repair mechanisms that result in alterations in the DSB regions or adjacent. CRISPR/Cas9 technology has transformed genetic modifications since its inception, and it is now routinely used to improve the genomics of large numbers of
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S. Elton, Terry, Md Ismail Hossain, Jessika Carvajal-Moreno, Xinyi Wang, Dalton J. Skaggs, and Jack C. Yalowich. "Maximizing the Efficacy of CRISPR/Cas Homology-Directed Repair Gene Targeting." In CRISPR Technology - Recent Advances [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.109051.

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Clustered regularly interspaced short palindromic repeats/CRISPR-associated system (CRISPR/Cas) is a powerful gene editing tool that can introduce double-strand breaks (DSBs) at precise target sites in genomic DNA. In mammalian cells, the CRISPR/Cas-generated DSBs can be repaired by either template-free error-prone end joining (e.g., non-homologous end joining/microhomology-mediated end joining [NHEJ]/[MMEJ]) or templated error-free homology-directed repair (HDR) pathways. CRISPR/Cas with NHEJ/MMEJ DNA repair results in various length insertions/deletion mutations (indels), which can cause fra
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Kumari, Esha, Sneha Kumari, Sai Santosh Das, and Laxmi Kanta Panigrahi. "CURRENT STATUS AND FUTURE PROSPECTS OF GENETICALLY MODIFIED CROP PLANTS IN INDIA." In Futuristic Trends in Biotechnology Volume 3 Book 4. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bjbt4p2ch9.

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By introducing advantageous foreign genes or blocking the expression of endogenous gene(s) in agricultural plants, genetic engineering and plant transformation have dramatically enhanced harvests. Herbicide tolerance, insect resistance, abiotic stress tolerance, disease resistance, and nutritional enhancement are all positive features that crops with genetic modification may have. 32 crops with more than 525 distinct transgenic events have been approved for production worldwide as of this writing. It has been demonstrated that transgenic technology increases agricultural yields, lowers pestici
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