Log in

Relevant bibliographies by topics / DNA hydrolysis

Contents

Journal articles
Dissertations / Theses
Books
Book chapters
Conference papers

Academic literature on the topic 'DNA hydrolysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'DNA hydrolysis.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "DNA hydrolysis"

1

Fekry, Mostafa I., and Kent S. Gates. "DNA-catalyzed hydrolysis of DNA phosphodiesters." Nature Chemical Biology 5, no. 10 (October 2009): 710–11. http://dx.doi.org/10.1038/nchembio.224.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Franklin, Sonya J. "Lanthanide-mediated DNA hydrolysis." Current Opinion in Chemical Biology 5, no. 2 (April 2001): 201–8. http://dx.doi.org/10.1016/s1367-5931(00)00191-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Zhou, Cong, Joshua L. Avins, Paul C. Klauser, Benjamin M. Brandsen, Yujeong Lee, and Scott K. Silverman. "DNA-Catalyzed Amide Hydrolysis." Journal of the American Chemical Society 138, no. 7 (February 15, 2016): 2106–9. http://dx.doi.org/10.1021/jacs.5b12647.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Chandra, Madhavaiah, Amit Sachdeva, and Scott K. Silverman. "DNA-catalyzed sequence-specific hydrolysis of DNA." Nature Chemical Biology 5, no. 10 (August 16, 2009): 718–20. http://dx.doi.org/10.1038/nchembio.201.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Ott, R., and R. Krämer. "DNA hydrolysis by inorganic catalysts." Applied Microbiology and Biotechnology 52, no. 6 (November 22, 1999): 761–67. http://dx.doi.org/10.1007/s002530051588.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Zilhadia, Zilhadia, Afifah Nurul Izzah, and Ofa Suzanti Betha. "Perbandingan Metode SYBR Green dan Hydrolysis Probe dalam Analisis DNA Gelatin Sapi dan Gelatin Babi Menggunakan Real Time Polymerase Chain Reaction." Jurnal Sains Farmasi & Klinis 4, no. 1 (December 15, 2017): 16. http://dx.doi.org/10.29208/jsfk.2017.4.1.194.

Full text

Abstract:

Pemanfaatan gelatin secara luas menimbulkan kontroversi dan kekhawatiran bagi masyarakat muslim karena pada umumnya gelatin terbuat dari kulit babi dan sapi. Salah satu teknik analisis yang dapat membedakan gelatin sapi dan gelatin babi adalah Real Time Polymerase Chain Reaction (PCR). Real Time PCR merupakan metode analisis berbasis DNA yang handal, efektif, dan terpecaya. Dalam analisis kualitatif dan kuantitatif, Real Time PCR membutuhkan pewarna fluoresens. Pewarna fluoresens yang umum digunakan adalah SYBR green dan hydrolysis probe. Telah dilakukan perbandingan antara metode SYBR green dan hydrolysis probe dalam analisis DNA gelatin menggunakan Real Time PCR. DNA pada gelatin diisolasi menggunakan kit komersial. Isolat DNA gelatin sapi dan DNA gelatin babi didapatkan sebanyak 19,38 ng/μl dan 13,63 ng/μl dengan kemurnian 1,566 dan 1,573. Isolat DNA yang dianalisis dengan metode SYBR green menggunakan suhu annealing 65o C untuk primer sapi dan suhu annealing 60o C untuk primer babi. Isolat DNA yang dianalisis dengan metode hydrolysis probe menggunakan suhu annealing 60o C untuk primer babi dan primer sapi. Hasil analisis dari kedua metode menunjukkan bahwa metode hydrolysis probe lebih spesifik dalam mengidentifikasi DNA pada gelatin dibandingkan menggunakan metode SYBR green.

APA, Harvard, Vancouver, ISO, and other styles

7

Koehler, D. R., and P. C. Hanawalt. "Digestion of damaged DNA by the T7 DNA polymerase-exonuclease." Biochemical Journal 293, no. 2 (July 15, 1993): 451–53. http://dx.doi.org/10.1042/bj2930451.

Full text

Abstract:

We have investigated the 3′-5′-exonuclease activity of phage T7 DNA polymerase for its usefulness as an approach for the detection of lesions in DNA. Unlike the T4 DNA polymerase-exonuclease, which is commonly used to map the position and frequency of lesions in very small DNA fragments, T7 DNA polymerase-exonuclease is able to hydrolyse almost completely the large fragments from KpnI-restricted mammalian DNA. However, we found that the exonuclease was also able to hydrolyse DNA containing several kinds of lesions: cyclobutane pyrimidine dimers, thymine glycols, and mono-adducts of 4′-hydroxymethyl-4,5′,8-trimethylpsoralen and 5′-methyl-isopsoralen. Modifications of the reaction conditions did not significantly alter the extent of hydrolysis. These properties distinguish the T7 DNA polymerase-exonuclease from the T4 DNA polymerase-exonuclease and make the T7 DNA polymerase-exonuclease unsuitable for detecting several types of lesions in DNA.

APA, Harvard, Vancouver, ISO, and other styles

8

Jackson, A. P., and A. Maxwell. "Identifying the catalytic residue of the ATPase reaction of DNA gyrase." Proceedings of the National Academy of Sciences 90, no. 23 (December 1, 1993): 11232–36. http://dx.doi.org/10.1073/pnas.90.23.11232.

Full text

Abstract:

We propose a mechanism for the hydrolysis of ATP by the DNA gyrase B protein in which Glu42 acts as a general base and His38 has a role in aligning and polarizing the glutamate residue. We have tested this mechanism by site-directed mutagenesis, converting Glu42 to Ala, Asp, and Gln, and His38 to Ala. In the presence of wild-type A protein, B proteins bearing the mutations Ala42 and Gln42 show no detectable supercoiling or ATPase activities, while Asp42 and Ala38 proteins have reduced activities. In the DNA cleavage and relaxation reactions of gyrase, which do not require ATP hydrolysis, wild-type and mutant proteins have similar activities. When the 43-kDa N-terminal fragment of the gyrase B protein (which hydrolyzes ATP) contained the mutations Ala42 or Gln42, ATP was bound but not hydrolyzed, supporting the idea that Glu42 is involved in hydrolysis but not nucleotide binding.

APA, Harvard, Vancouver, ISO, and other styles

9

Unciuleac, Mihaela-Carmen, Aviv Meir, Chaoyou Xue, Garrett M. Warren, Eric C. Greene, and Stewart Shuman. "Clutch mechanism of chemomechanical coupling in a DNA resecting motor nuclease." Proceedings of the National Academy of Sciences 118, no. 11 (March 8, 2021): e2023955118. http://dx.doi.org/10.1073/pnas.2023955118.

Full text

Abstract:

Mycobacterial AdnAB is a heterodimeric helicase–nuclease that initiates homologous recombination by resecting DNA double-strand breaks (DSBs). The N-terminal motor domain of the AdnB subunit hydrolyzes ATP to drive rapid and processive 3′ to 5′ translocation of AdnAB on the tracking DNA strand. ATP hydrolysis is mechanically productive when oscillating protein domain motions synchronized with the ATPase cycle propel the DNA tracking strand forward by a single-nucleotide step, in what is thought to entail a pawl-and-ratchet–like fashion. By gauging the effects of alanine mutations of the 16 amino acids at the AdnB–DNA interface on DNA-dependent ATP hydrolysis, DNA translocation, and DSB resection in ensemble and single-molecule assays, we gained key insights into which DNA contacts couple ATP hydrolysis to motor activity. The results implicate AdnB Trp325, which intercalates into the tracking strand and stacks on a nucleobase, as the singular essential constituent of the ratchet pawl, without which ATP hydrolysis on ssDNA is mechanically futile. Loss of Thr663 and Thr118 contacts with tracking strand phosphates and of His665 with a nucleobase drastically slows the AdnAB motor during DSB resection. Our findings for AdnAB prompt us to analogize its mechanism to that of an automobile clutch.

APA, Harvard, Vancouver, ISO, and other styles

10

Singh, Reema, Sumudu R. Perera, George S. Katselis, Paulos Chumala, Irene Martin, Anthony Kusalik, Kristen M. Mitzel, and Jo-Anne R. Dillon. "A β-lactamase-producing plasmid from Neisseria gonorrhoeae carrying a unique 6 bp deletion in blaTEM-1 encoding a truncated 24 kDa TEM-1 penicillinase that hydrolyses ampicillin slowly." Journal of Antimicrobial Chemotherapy 74, no. 10 (July 23, 2019): 2904–12. http://dx.doi.org/10.1093/jac/dkz306.

Full text

Abstract:

AbstractBackgroundSeven structurally related β-lactamase-producing plasmids have been characterized in penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates. We characterized a variant (i.e. pJRD20, Canada type) of the Africa-type (pJD5) plasmid isolated from N. gonorrhoeae strain 8903.ObjectivesTo compare the DNA sequence of pJRD20 with that of pJD5 and pJD4 (Asia-type) and their TEM-1 β-lactamases.MethodsN. gonorrhoeae 8903 was identified as part of the Gonococcal Antimicrobial Surveillance Program in Canada. β-Lactamase production was assessed using nitrocefin. MICs were determined by agar dilution and Etest methods (CLSI). The DNA sequences of pJRD20, pJD5 and pJD4 were assembled and annotated. The structure of TEM-1 and its penicillin-binding properties were determined by in silico molecular modelling and docking. TEM-1 proteins were characterized by western blot, mass spectrometry and ampicillin hydrolysis assays.ResultsN. gonorrhoeae 8903 exhibited intermediate susceptibility to penicillin with slow β-lactamase activity (i.e. 35 min to hydrolyse nitrocefin). Except for a novel 6 bp deletion starting at the G of the ATG start codon of blaTEM-1, the DNA sequence of pJRD20 was identical to that of pJD5. The TEM-1 β-lactamase produced by pJRD20 is 24 kDa and hydrolyses ampicillin only after several hours.ConclusionsThis unusual PPNG isolate might have been characterized as a non-PPNG owing to its low MIC of penicillin and its very slow hydrolysis of nitrocefin. Given the unusual nature of its TEM-1 β-lactamase, laboratories might consider extending the duration of nitrocefin hydrolysis assays.

APA, Harvard, Vancouver, ISO, and other styles

More sources

Dissertations / Theses on the topic "DNA hydrolysis"

1

Evans, Steven John. "Structure, function and mechanism of action of bovine pancreatic deoxyribonuclease I : role of amino acid residues involved in phosphate contacts." Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321857.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Przybylski, Jennifer L., and University of Lethbridge Faculty of Arts and Science. "Computational modeling of the hydrolysis of 2'-deoxyribonucleic acids." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009, 2009. http://hdl.handle.net/10133/1292.

Full text

Abstract:

The mechanism for the hydrolysis of 2′-deoxyribonucleosides is examined using computational chemistry techniques. Initially, a model capable of accurately predicting the mechanism and activation barrier for the uncatalyzed hydrolysis of 2′-deoxyuridine is designed. It is found that the smallest model includes both explicit and implicit solvation during the optimization step. Next, this hybrid solvation model is applied to four natural nucleosides, namely 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine and thymidine. The hybrid model correctly predicts the trend in activation Gibbs energies for the pyrimidines and purines, separately. Finally, the concepts developed during the generation of the uncatalyzed hydrolysis model are applied to the mechanism of action of a glycosylase enzyme, namely human uracil DNA glycosylase. A hybrid ONIOM approach is utilized to study the experimentally proposed two-step mechanism. Results regarding the protonation state of His148 are inconclusive, and future directions are proposed.
xiii, [131] leaves : ill. (some col.) ; 29 cm

APA, Harvard, Vancouver, ISO, and other styles

3

Eissa, Omaima Abdel-Latif Elkotb. "Cloning of a novel operon containing genes for 4-#alpha#-glucanotransferase, maltodextrin phosphorylase, and a regulatory protein from Clostridium butyricum." Thesis, University of Southampton, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296246.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Marrone, April. "THE BIOCHEMICAL REACTIONS OF DRY STATE DNA." Doctoral diss., University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3622.

Full text

Abstract:

The biochemistry of dry state DNA is of interest to the fields of forensics, ancient DNA, and DNA storage. The exact chemical nature of the degradation of the DNA molecule in the dry state has not been studied prior. If determined what chemical changes the DNA molecule undergoes, to what degree and in what time frame then protocols can be implemented to bypass the impact of this damage or to repair it when necessary. It is suspected that similar reactions occur to the dry state DNA molecule as does to the hydrated molecule. It cannot be assumed, however that these types of chemical processes occur to the same extent and at the same rates. In general the generic process of hydrolysis encompasses two important reactions, that of deamination and of base loss from the 2’-deoxyribose backbone. Base loss is believed to ultimately lead to chain scission. It is also suspect that reactive oxygen species (ROS) have an important role in the chemistry associated with DNA. Species such as hydroxyl radicals (OH·) and singlet oxygen (¹O₂) can lead to strand scissions and chemically modified bases. Throughout this project various techniques were used to determine damage to DNA and its molecular constituents under conditions leading to hydrolytic and oxidative damage. Novel techniques used in this study include ion-pairing chromatography and denaturing HPLC (DHPLC) to measure glycosidic bond cleavage and strand breaks. The extent to which the macromolecule haemoglobin (Hb) can lead to oxidative damage of DNA in dried blood stains by acting as a Fenton chemistry catalyst was evaluated. Additionally the enzymatic activity of the extracellular nuclease from Alteromonas espejiana, BAL 31 was studied as it pertains to the degradation of single-stranded short homopolymeric oligonucleotides. This study serves as the basis for future, more in depth experimentation into the more specific nature of dry state DNA biochemistry. It was found that to a large extent the same degradation reactions (base hydrolysis, base modifications, and strand breaks) do occur in the dry state as in the hydrated state when heat and UV radiation are used as energy sources. Reaction rates indicate that base hydrolysis and deamination occur much more slowly, yet have the same energies of activation in both states. Single strand breaks of dry state duplex DNA occur with a half life of 24 ± 2 days and appears to occur in a mechanistic manner which could be of interest when attempting to repair such damage. In addition, base loss alone does not correlate with the extent of single strand breaks detected. Thermodynamic data can lead to the conclusion that DNA degradation in both dry and hydrated states is not a spontaneous process. It is also concluded that though the Hb molecule undergoes oxidative changes over time, these changes do not impact its ability to become a more aggressive Fenton reagent. However, the presence of Hb in the vicinity of DNA does create the opportunity for OH· induced damage to the deoxyribose sugar, and most likely the DNA bases themselves. This study also reveals that the general purpose BAL 31 nuclease commonly used in molecular genetics exhibits a hithertofore non-characterized degree of substrate specificity with respect to single-stranded DNA oligomers. Specifically, BAL 31 nuclease activity was found to be affected by the presence of guanine in ssDNA oligomers.
Ph.D.
Department of Chemistry
Sciences
Chemistry PhD

APA, Harvard, Vancouver, ISO, and other styles

5

Bhat, Anayat [Verfasser]. "Live Cell Fluorescence Imaging of Nucleotide Dynamics : ATP Hydrolysis and DNA Damage Response / Anayat Bhat." Konstanz : KOPS Universität Konstanz, 2021. http://d-nb.info/1229351094/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Marin, Córdoba Roberto. "Chromium carcinogenesis characterization of DNA damaging intermediates by EPR ³¹P NMR, HPLC, ESI-MS and magnetic susceptibility /." Ohio : Ohio University, 2010. http://www.ohiolink.edu/etd/view.cgi?ohiou1261417590.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Moysan-Le, Meur Annie. "Caracterisation de dosage des produits de photoaddition de psoralene dans l'adn "in vitro" et dans l'adn cellulaire." Paris 6, 1987. http://www.theses.fr/1987PA066021.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Marin, Cordoba Roberto. "Chromium Carcinogenesis: Characterization of DNA damaging Intermediates by EPR ³¹P NMR, HPLC, ESI-MS and Magnetic Susceptibility." Ohio University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1261417590.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Taori, Vijay P. "Poly(glycoamidoamine)s: Understanding their Structure and Structure-Bioactivity Relationships." Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/77982.

Full text

Abstract:

In order to achieve efficient therapeutic effect, it is important to understand the structure of biomaterials that are used in the therapeutic delivery system. This dissertation is dedicated towards understanding the hydrolysis pattern of plasmid DNA (pDNA) delivery vehicles comprised of poly(glycoamidoamine)s (PGAAs) under physiological conditions and effects of subtle changes in the chemical structure of the PGAAs on its biological performance. The unusual hydrolysis of the tartarate and galactarate based PGAAs was investigated by studying the hydrolysis of small model molecules which mimic the repeat unit of the respective polymers. In the case of galactarate and tartarate based molecules with terminal amines showed faster hydrolysis of the amide bonds. In addition for the tartarate based compounds, it was also found that it is necessary to have terminal amine functionality for the intramolecular hydrolysis to occur. The model compounds consists of two amide bonds and were designed symmetric, however amide bond on only one side of the tartarate moiety show underwent hydrolysis. Further studies show that one side of the amine assists the hydrolysis of the amide bond on the other side of the tartarate moiety. The degradation of poly(L-tartaramidopentaethylenetetramine) (T4) was also used to study the sustained release of pDNA from the layer-by-layer constructs of T4/pDNA. The thickness of the constructs was characterized by ellipsometry while the UV-visible spectroscopy was used to characterize the loading capacity of the constructs for pDNA. The indirect sustained release of pDNA under the physiological conditions with respect to time was characterized by the cellular uptake studies in HeLa cells. The increase in the uptake of the Cy5 labeled pDNA was seen at extended period of eleven days. The integrity of the sustained released pDNA for the transgene expression was characterized with an assay to see the expression of the green fluorescent protein (GFP) from the T4/GFP-pDNA layer-by-layer constructs. PGAAs show a very efficient delivery of the pDNA in a non-toxic manner. The chemical structure of the polymer can dictate the binding with pDNA and also the release of the pDNA form the polymer-pDNA complexes. In order to better understand the fundamentals of the nucleic acid delivery and to better design the nucleic acid delivery vehicles, subtle changes in the chemical structure of the PGAAs were designed and studied for the biological activity. The effect of charge type was investigated by designing and synthesizing guanidine based polymer series analogues to galactarate and tartarate based PGAAs (G1 and T1) which incorporate secondary amines as the charge type on the polymer backbone. The guanidine based polymer series, poly(glycoamidoguanidine)s (PGAGs), show very non toxic behavior in HeLa cells at all the different polymer to pDNA ratio (N/P ratio) studied. Interestingly PGAGs are the only non-toxic guanidine containing polymers which are reported in the literature to the date. The cellular uptake of pDNA assisted from the PGAGs is a little higher than PGAAs compared although both the series of polymers show similar transgene expression. The transgene expression in case of PGAGs also imply the release of the polymer-pDNA complexes from the endosome. In another study of structure-bioactivity relationship based on the degree of polymerization (DP) of poly(galactaramidopentaethylenetetramine) (G4), it was found that the increase in the DP of G4 increases the toxicity of the polymers in the HeLa cells.
Ph. D.

APA, Harvard, Vancouver, ISO, and other styles

10

Incardona, Marie-Françoise. "Mesure de lésions de type oxydatif de l'ADN dans les fluides biologiques et dans l'ADN isolé." Grenoble 1, 1994. http://www.theses.fr/1994GRE10162.

Full text

Abstract:

Divers facteurs (stress oxydant, exposition a divers rayonnements) peuvent engendrer des modifications des bases de l'adn. La mise au point de methodes d'analyse suffisamment fiables et sensibles nous a permis de detecter et de doser plusieurs dommages oxydatifs dans un fluide biologique (urine humaine) et dans l'adn isole. Pour cela, deux techniques ont ete respectivement mises en uvre: la chromatographie gazeuse couplee a la spectrometrie de masse (cg/sm) et la chromatographie liquide a haute performance associee a une detection electrochimique (clhp/ec). La mise au point de methodes de detection de dommages oxydatifs dans l'urine par cg/sm a ete appliquee a la 5-hydroxymethyluracile, la 5-hydroxyuracile et a leurs nucleosides. Ce travail a implique, la synthese d'etalons internes enrichis isotopiquement (#2h, #1#5n, #1#8o) pour une mesure quantitative des lesions, la mise au point de la technique d'extraction du defaut et enfin l'optimisation des parametres de cg/sm pour l'obtention d'une reponse optimale. Un deuxieme volet du travail a concerne le developpement d'une nouvelle methode d'analyse de bases modifiees utilisant une colonne chromatographique a phase normale (nh#2) couplee a un detecteur electrochimique. Celle-ci nous a permis de detecter plusieurs molecules hydrophiles (5-hydroxyuracile, 5-hydroxycytosine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine et 4,6-diamino-5-formamidopyrimidine) jusqu'a present difficiles a mesurer dans les conditions usuelles (colonne c18). Nous avons applique cet outil a la recherche et au dosage de la 5-hydroxyuracile et de la 5-hydroxycytosine dans l'adn soumis a deux conditions de stress oxydant (radiations ionisantes et photooxydation sensibilisee). La recherche de ces bases oxydees dans l'adn a ete facilitee par une nouvelle methode d'hydrolyse

APA, Harvard, Vancouver, ISO, and other styles

More sources

Books on the topic "DNA hydrolysis"

1

Estes, Patricia S. Cardiovascular and respiratory responses of the ghost shrimp, Callianassa californiensis Dana, to the pesticide carbaryl and its hydrolytic product 1-naphthol. 1986.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

2

1938-, Lifshitz Fima, and Mead Johnson & Company. Nutritional Division., eds. Nutrition for special needs in infancy: Protein hydrolysates. New York: M. Dekker, 1985.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "DNA hydrolysis"

1

Williams, N. H. "DNA Hydrolysis: Mechanism and Reactivity." In Artificial Nucleases, 3–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18510-6_2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Testillano, P. S., M. C. Risueño, M. A. Ollacarizqueta, and C. J. Tandler. "Selective Staining of DNA at the Ultrastructural Level After Alkaline Hydrolysis." In Nuclear Structure and Function, 477–81. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0667-2_98.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Komiyama, Makoto, Naoya Takeda, and Makoto Irisawa. "Synergism of Two Metal Ions for the Hydrolysis of DNA and RNA." In DNA and RNA Cleavers and Chemotherapy of Cancer and Viral Diseases, 321–35. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-0251-0_22.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Guengerich, Frederick Peter. "Hydrolytic, Deamination, and Rearrangement Reactions of DNA Adducts." In Molecular Life Sciences, 535–38. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4614-1531-2_376.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Guengerich, F. Peter. "Hydrolytic, Deamination, and Rearrangement Reactions of DNA Adducts." In Molecular Life Sciences, 1–4. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6436-5_376-1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Orebaugh, Clinton D., Scott A. Lujan, Adam B. Burkholder, Anders R. Clausen, and Thomas A. Kunkel. "Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing." In Methods in Molecular Biology, 329–45. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7306-4_23.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Lindsley, Janet E. "Type II DNA topoisomerases: Coupling directional DNA transport to ATP hydrolysis." In Energy Coupling and Molecular Motors, 371—IX. Elsevier, 2003. http://dx.doi.org/10.1016/s1874-6047(04)80009-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Bernstein, Harris, and Carol Bernstein. "Origin of DNA Repair in the RNA World." In DNA Repair [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.93822.

Full text

Abstract:

The early history of life on Earth likely included a stage in which life existed as self-replicating protocells with single-stranded RNA (ssRNA) genomes. In this RNA world, genome damage from a variety of sources (spontaneous hydrolysis, UV, etc.) would have been a problem for survival. Selection pressure for dealing with genome damage would have led to adaptive strategies for mitigating the damage. In today’s world, RNA viruses with ssRNA genomes are common, and these viruses similarly need to cope with genome damage. Thus ssRNA viruses can serve as models for understanding the early evolution of genome repair. As the ssRNA protocells in the early RNA world evolved, the RNA genome likely gave rise, through a series of evolutionary stages, to the double-stranded DNA (dsDNA) genome. In ssRNA to dsDNA evolution, genome repair processes also likely evolved to accommodate this transition. Some of the basic features of ssRNA genome repair appear to have been retained in descendants with dsDNA genomes. In particular, a type of strand-switching recombination occurs when ssRNA replication is blocked by a damage in the template strand. Elements of this process appear to have a central role in recombinational repair processes during meiosis and mitosis of descendant dsDNA organisms.

APA, Harvard, Vancouver, ISO, and other styles

9

Crain, Pamela F. "[42] Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry." In Mass Spectrometry, 782–90. Elsevier, 1990. http://dx.doi.org/10.1016/0076-6879(90)93450-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Frey, Perry A., and Adrian D. Hegeman. "Glycosyl Group Transferases." In Enzymatic Reaction Mechanisms. Oxford University Press, 2007. http://dx.doi.org/10.1093/oso/9780195122589.003.0016.

Full text

Abstract:

Glycosyl group transfer underlies the biosynthesis and breakdown of all nucleotides, polysaccharides, glycoproteins, glycolipids, and glycosylated nucleic acids, as well as certain DNA repair processes. Glycosyl transfer consists of the transfer of the anomeric carbon of a sugar derivative from one acceptor to another, as in, which describes the transfer of a generic pyranosyl ring between nucleophilic atoms :X and :Y of acceptor molecules. The stereochemistry at the anomeric carbon is not specified in eq. 12-1, but the leaving group occupies the axial position in an α-anomer or the equatorial position in a β-anomer. The overall transfer can proceed with either retention or inversion of configuration. In biochemistry, the acceptor atoms can be oxygen, nitrogen, sulfur, or in the biosynthesis of C-nucleosides even carbon. The great majority of biological glycosyl transfer reactions involve transfer between oxygen atoms of different acceptor molecules. Enzymes catalyzing glycosyl transfer are broadly grouped according to whether the acceptor :Y–R2 in is water or another molecule. In the actions of glycosidases, the acceptor is water, and glycosyl transfer results in hydrolysis of a glycoside, a practically irreversible process in dilute aqueous solutions. In the action of glycosyltransferases, the acceptors are molecules with hydroxyl, amide, amine, sulfhydryl, or phosphate groups. The simplest nonenzymatic glycosyl transfer reaction is the hydrolysis of a glycoside, and early studies revealed the fundamental fact that glycosides are much less reactive toward hydrolysis in basic solutions than in acidic solutions. This fact underlies much that is known about the mechanism of glycosyl transfer; that is, the anomeric carbon of a glycoside is remarkably unreactive toward direct nucleophilic attack, but it becomes reactive when one of the oxygens is protonated by an acid, as illustrated in fig. 12-1 for the acid-catalyzed hydrolysis of a generic glycoside. The reaction by both mechanisms in fig. 12-1 proceeds by pre-equilibrium protonation of the glycoside to form oxonium ion intermediates, which are subject to hydrolysis by water. The two mechanisms in fig. 12-1 are of interest. The mechanism proceeding through exocyclic cleavage of the glycoside has historically been regarded as the more likely, and for this reason, the route through endocyclic cleavage has received little consideration.

APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "DNA hydrolysis"

1

GANDELMAN, O., LC TISI, PJ WHITE, JAH MURRAY, and DJ SQUIRRELL. "BIOLUMINESCENT DETECTION OF RNA HYDROLYSIS PROBES IN DNA TESTING." In Proceedings of the 13th International Symposium. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702203_0126.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Kurita, Hirofumi, Ken-ichi Inaishi, Ken Torii, Madoka Urisu, Michihiko Nakano, Shinji Katsura, and Akira Mizuno. "Real-time direct observation of single-molecule DNA hydrolysis by exonucleaseIII." In 2006 IEEE International Symposium on Micro-NanoMechatronics and Human Science. IEEE, 2006. http://dx.doi.org/10.1109/mhs.2006.320277.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Kurita, Hirofumi, Ken Torii, Hachiro Yasuda, Kazunori Takashima, Shinji Katsura, and Akira Mizuno. "Single-molecule Observation of DNA Hydrolysis by ExonucleaseIII; Effect of Physical Form of DNA on Exonuclease Reaction." In 2007 International Symposium on Micro-NanoMechatronics and Human Science. IEEE, 2007. http://dx.doi.org/10.1109/mhs.2007.4420842.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Krasnoshtanova, Alla, and Elisaveta Borovkova. "OBTAINING NUCLEIC ACID PREPARATIONS AND THEIR HYDROLYSATES FROM BIOMASS OF METHANE-OXIDIZING BACTERIA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/14.

Full text

Abstract:

"Due to the unfavourable environmental, social and economic situation, the need for the treatment of oncological diseases and diseases associated with impaired activity of the immune system is increasing. A lot of these drugs are made on the basis of nucleic acid components, the industrial production of which is practically non-existent in Russia. Therefore, a task of current interest is to develop the basis of the technology for obtaining components of nucleic acids, which can be widely used in medicine as immunomodulatory, wound-healing, antiviral, and diagnostic medicine, as well as for cancer treatment. Most of the described in literature methods of isolating nucleic acid components from plant, animal and microbial raw materials are based on the use of toxic and expensive organic solvents, that’s why it is impossible to apply these methods outside of laboratory conditions. The most promising source of raw materials for nucleic acids is the biomass of microorganisms (yeast and bacteria) from biomass, since the use of such source makes it possible to quickly obtain a large enough amount of biomass, and, consequently, a larger amount of nucleic acids. This allows obtaining DNA in addition to RNA. RNA and DNA substances can be used to obtain nucleosides and nitrogenous bases, which are also widely used in medicine. The purpose of these studies was to select the conditions for the extraction of RNA and DNA from the biomass of methane-oxidizing bacteria in one technological cycle, as well as to compare the efficiency of alkaline and acid hydrolysis of microbial RNA and DNA. The need for a two-stage extraction of nucleic acids from the biomass of methane-oxidizing bacteria in order to separately extract RNA and DNA was Substantiated. It was ascertained that at the first stage of extraction at a temperature of 90 ° C, pH 9.0 for 90 min, at least 85% of RNA is extracted. After the separation of the extract by centrifugation, the partially denuclearized biomass must be re-processed under the same conditions in order to extract DNA by at least 83%. The modes of concentration of RNA and DNA solutions by ultrafiltration were selected. It was found that in order to achieve effective deposition of nucleic acids at the isoelectric point, the concentration of the RNA solution must be carried out on the UPM-10 membrane at the concentration degree of 7, and the DNA solution on the UPM-100 membrane at the concentration degree 6. The dynamics of decomposition of nucleic-protein complexes in the medium of monoammonium phosphate was investigated. It was shown that the transition of NA into solution by at least 80% is achieved at a monoammonium phosphate concentration of 1.7 M, a temperature of 55 ° C for 90 min. The use of 5-fold washing of oligonucleotide substances with acidified water (pH 2.0) to remove excess mineral impurities was substantiated. А comparative assessment of acid and alkaline hydrolysis of RNA and DNA was carried out in order to obtain derivatives of nucleic acids."

APA, Harvard, Vancouver, ISO, and other styles

5

Liao, Jung-Chi, and George Oster. "The Engines of Biomolecular Motors." In ASME 2004 3rd Integrated Nanosystems Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/nano2004-46094.

Full text

Abstract:

The majority of biomolecular motors are powered by nucleoside triphosphate (NTP), especially adenosine triphosphate (ATP). These motors consist of a β-sheet with highly conserved motifs and the nucleotide binding domain around it. The highly conserved protein folds are the engines of these motors, which convert the energy of NTP hydrolysis cycle to mechanical work. Although functions of molecular motors are widely diverse, (including cargo movement, DNA unwinding, protein degradation, ion pumping, etc), the nucleotide binding domains are very similar. In the binding site, NTP undergoes a hydrolysis cycle E+NTP⇄E·NTP⇄E•NTP⇄E•NDP•Pi⇄E•NDP+Pi⇄E+NDP+Pi where E is the enzyme (motor protein), the small dot represents the docking of NTP, and the large dot represents the tightly-bound states. The hydrogen bond network formed in the NTP binding step, as shown in Figure 1 [1], deforms the β-sheet and adjacent structures. The local deformation propagates to conformational changes of functional residues to do mechanical work or to change the affinity to the substrate [2]. For multimeric motor proteins, we must also consider the stress paths among subunits which control the sequence and the activity of the protein. Stress trajectories emanating from a binding site either passes through a circumferential stress loop or a stress loop through the substrate.

APA, Harvard, Vancouver, ISO, and other styles

6

Homes, W. E., H. R. Lijnen, L. Nelles, C. Kluft, and D. Collen. "AN ALANINE INSERTION IN α2-ANTIPLASMIN ‘ENSCHEDE’ ABOLISHES ITS PLASM IN INHIBITORY ACTIVITY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642897.

Full text

Abstract:

Congenital deficiencies of the fibrinolytic inhibitor α2antiplasmin (α2AP) may result in bleeding disorders. An abnormal a AP (α2AP‘Enschede’) is known. 2 siblings with 3% functional activity and normal antigen level have parents with 50% activity and normal antigen. The protein interacts normally with the lysine-binding site(s) of plasmin(ogen) but does not inhibit plasmin irreversibly. α2AP Enschede is a plasmin substrate that like the normal protein releases a M 8,000 peptide upon reaction with plasmin. In the present study, Southern blot analysis, using an α2AP cDNA probe showed a restriction fragment length polymorphism within a small genomic DNA fragment of the Enschede family members. Cloning and sequencing of these fragments revealed a GCG inframe insertion that results in an alanine addition between amino acids 353 and 357, 7-10 positions NH -terminal to the reactive site PI residue, Arg364. This area is homologous to the A4 B-sheet of reactive site cleaved a -antitrypsin. Clones from each individual confirm the parents as true heterozygotes and the children as true homozygotes. A cloned genomic DNA sequence containing the insertion (V ) was exchanged for the normal sequence in a eukaryotic a AP expression plasmid. Recombinant α2AP‘Enschede’ (ra AfVAla) purified from the conditioned media of transfected Chinese Hamster Ovary Cells is analogous to plasma a α2AP‘Enschede’ with respect to interactions with plasmin and plasminogen. Preliminary analysis of the released Mr 8,000 recombinant peptide shows that its NH -terminus is the same as the peptide cleaved from normal a AP. Although ra α2APVAla does not inhibit plasmin irreversibly it does, however, act as a competitive inhibitor of hydrolysis of the chromogenic substrate S-2251 by plasmin.The K for this interaction is 25 nM. Thus, α2APAla retains a high affinity for the active center of plasmin. In conclusion, an Ala insertion near the reactive site of α2AP must have resulted in a structural perturbation that has abolished the plasmin inhibitory activity of a α2AP‘Enschede’. This variant may provide a model for further investigation of structure-function relationships in the serpins which determine the relative inhibitor vs. substrate properties.

APA, Harvard, Vancouver, ISO, and other styles

7

Maria Ferreira Jardim Da Silveira, Jose, and Pedro Lucas Chagas MendonÇa. "Patents Trajectories Analysis: Enzymatic Hydrolysis and Bioethanol." In XXIII Congresso de Iniciação Científica da Unicamp. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.19146/pibic-2015-37737.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Reis Pedrosa, Nicolas, Aline Carvalho da Costa, and Daniele Longo Machado. "Sugarcane bagasse hydrolysis at high solids concentration." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-52036.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Lucas Chagas MendonÇa, Pedro, and Jose Maria Ferreira Jardim Da Silveira. "Patents Trajectories Analysis: Enzymatic Hydrolysis and Bioethanol." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-50630.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Sato, Letícia, Paulo José Samenho Moran, Lucidio Cristovão Fardelone, and José Augusto Rosário Rodrigues. "Screening of Lipases for Enantioselective Hydrolysis of 1-butiryloxyarylphosphonates." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-51226.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!